Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005400

Physical Location & Seq

Genomic Coordinates Forward (+)

34421149 .. 34429456

Gene Structure 8308 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1285000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3057 bp

ATGGCGGTGGCGCCTCTTCCTCTGGTGAAACATCATCAGCTGTCACCTCTGTGCTCCAAGTTTTCCCACCCCCTCAACCTTAAAAACCGGCAGCTGGGTTCCTCCTTTTTCGGCGGAAGAACCTTCCAAATGCCCGAAACATCGAGAATGGTGTGCCGAAGAAGACGAAGAATGCAAGCAGTGGCTTCGTTGGGAGGTTTATTAGGTGGGATTTTCAAAGGAAGCGACACTGGTGAGTCCACCAGGCAGCAGTATGCTCCCACCGTGTCTGTCATCAATGGCTTGGAAGCTCAGATGTCTGCGCTCTCCGATTCGGAGCTGAGGGAGAAGACGCGCCTCTTTCAAGAACGCGCCAAGCAGGGAGAATCTTTGGATTCCCTTTTACCTGAAGCATTTGCCGTCGTAAGGGAGGCTTCGAAGAGGGTTTTAGGCCTTCGTCCCTTTGATGTCCAACTCATAGGTGGCATGGTTCTTCATAAGGGGGAAATAGCTGAAATGAGAACTGGAGAAGGAAAAACCCTTGTTGCTATCTTACCAGCATATTTAAATGCATTAATTGGAAAAGGAGTTCATGTTGTTACTGTCAACGATTATCTGGCACGGCGGGATTGTGAGTGGGTTGGCCAGGTTCCTCGTTTCCTTGGATTGAAGGTTGGCCTAATCCAACAGAATATGACAAGTGAGCAACGAAGGGAGAATTACTTATGCGACATCACATATGTTACCAACAGTGAGCTTGGTTTTGATTACTTGAGAGATAATCTGGCCACGGAAAAGATTAGTGTTGAGGAGCTTGTCTTGAGGAATTTCAATTACTGTGTAATTGACGAGGTTGATTCCATCCTTATTGATGAAGCAAGGACACCTCTCATTATATCTGGACCTGCAGAAAAACCTAGCGATCGGTATTATAAAGCTGCAAAGATAGCTGCAGTATTTGAGCGTGAAATACATTACACTGTGGATGAAAAGATGAAAACAGTTCTGCTCACAGAACAGGGTTATGAAGATTCGGAAGAGATTCTGGGTGTAAAAGATCTATATGATCCCCGAGAACAGTGGGCATCTTATGTTCTGAATGCAATAAAAGCGAAAGAACTCTTTCTTAGAGATGTAAACTATATCATCCGTGGAAAAGAGGTTCTTATTGTTGATGAGTTTACAGGTCGAGTTATGCAGGGGAGGCGCTGGAGTGATGGACTTCACCAAGCAGTTGAAGCAAAAGAAGGTCTGCCCATTCAAAATGAAACTGTGACACTGGCTTCAATTAGCTACCAAAATTTCTTTCTCCAGTTCCCAAAACTTTGTGGGATGACTGGCACTGCAGCAACTGAAAGCACAGAATTTGAAAGTATATACAAGCTTAAAGTTACAATTGTTCCAACAAACAAGCCTATGATTAGAAAGGATGAATCTGATGTAGTCTTCAGGGCAACTAAAGGAAAATGGCGGGCTGTTGTAGTAGAGATTTCTAGGATGAACAAGACAGGTCGTCCTGTACTTGTTGGTACAACTAGTGTTGAGCAGAGTGACTCATTGTCTGAGCAATTGCAAGAAGTTGGAATTCCCCATGAGGTTCTCAATGCAAAGCCAGAGAATGTGGAGAGGGAAGCAGAAATTGTGGCACAAAGTGGTCGTCTAGGGGCAGTGACTATTGCGACCAATATGGCAGGTCGAGGAACTGACATTATCCTTGGTGGGAATGCTGAATTTATGGCAAGGCTGAAGCTACGGGAGATGCTGATGCCAAGAGTTGTTAAACTAACAGAAGGTGGTTATGTGTCAGTGAAAAAACTTCCCCCAAAGAAATCATGGAAGGTTAATGAAAACCTATTTCCATGCAAACTATCCAATGAAAAGACCAAGTTGGCTGAGGAAGCTGTAAAGTTAGCTGTTGAAACGTGGGGTCAGAGGTCATTAAATGAGCTTGAAGCAGAGGAGCGTTTATCTTATTCTTGTGAAAAGGGCCCTGCTGAGGACCAAGTCATAGCCAAACTGCGGAGTGCATTTCTAGAAATTGTTAAAGAATATAAGGTCTACACAGAGGAAGAAAGAAAGAAGGTTGTGTCATCTGGCGGGCTTCATGTCGTGGGGACAGAACGCCATGAATCTCGTCGAATCGATAATCAGCTTCGTGGTCGAACTGGAAGGCAGGGGGATCCTGGAAGTTCTCGGTTCTTCTTAAGTCTTGAAGACAACATCTTTCGTATATTTGGTGGAGACCGTATTCAGGGTTTAATGAGAGCTTTCAGAGTTGAGGACCTGCCAATTGAGTCCAAGATGCTAACCAAAGCACTAGATGAAGCTCAACGGAAAGTGGAAAATTATTTCTTTGATATCCGAAAGCAATTATTTGAGTTTGATGAAGTGTTAAACAGCCAAAGAGACCGTGTCTATACGGAAAGAAGACGAGCCCTTGAATCTGACAATCTCCAATCACTTATTATTGAATATGCTGAACTAACAATGGATGATATCTTGGAGGCAAATATTGGGTCAGATGCTTCAAAAGAAAGTTGGGATCTTGAGAAGCTCATCAAGAAACTTCAACAATATTGCTACCTATTGAATGATTTGACACCGGACTTGCTGAGGAGCAATTGTTCAAGTTATGAGGATTTGCAGGAGTACCTTCGTCTTCGTGGTCGTGAAGCATATTTGCAGAAAAGGGATATTATTGAGAGCAAAGCACCAGGCTTGATGAAGGATGCTGAAAGGTTCCTAGTTTTAAGCAATATTGATCGCCTGTGGAAGGAGCACTTACAAGCACTTAAGTTTGTTCAACAGGCTGTTGGTTTACGTGGATACGCACAACGGGATCCACTTATTGAATACAAGCTTGAAGGCTATAATCTTTTCTTAGAGATGATGGCACAGATAAGAAGAAATGTTATATATTCCATATACCAGTTTCAACCGGTGATGGTAAAGAAGGATGGAGACCAAAGAGAGAATAACAATTCAGTTGTCACAAATGGTAGGAGGAGTAATAATAACCCTGATCCGTTGAGCTCTGTCGATCCTTCAGCAGCTGCGACCCCCCAATCTAGTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071806 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

1019

Amino Acids

115.51

Weight (kDa)

6.9

Isoelectric Point (pI)

45.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	79 - 450	6.5e-123	SecA DEAD-like domain
DEAD	PF00270	163 - 283	4.6e-07	DEAD/DEAH box helicase
SecA_PP_bind	PF01043	300 - 406	1.1e-37	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	465 - 754	2.7e-82	SecA P-loop domain
SecA_SW	PF07516	756 - 972	1.4e-56	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01800 AT4G01800 AT4G01800
fragaria_vesca	FvH4_1g26240
malus_domestica	MD17G1285000.v1.1
prunus_persica	Prupe.3G002700_v2.0.a1
pyrus_communis	pycom09g19850 pycom17g28080 pycom17g28090
rosa_chinensis	RchiOBHm_Chr3g0485781
rosa_laevigata	RLG00000023125
rosa_multiflora	Rmu_co8471925.1_g000001 Rmu_sc0012870.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123840
rosa_rugosa	Rorug03G0221400
rosa_samantha	Rh1AG180100 Rh1CG167100 Rh3AG270700 Rh3BG306200 Rh3BG306300 Rh3CG304600 Rh3DG300700 Rh4AG241300 Rh4AG241400 Rh4BG244600 Rh4BG244700 Rh4CG255400 Rh7CG119700
rosa_wichuraiana	Rw3G024110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	912
Acc36I	ACCTGC	3 cut(s)	892, 1661, 2271
AccB1I	GGYRCC	1 cut(s)	10
AccI	GTMKAC	1 cut(s)	2037
AccII	CGCG	2 cut(s)	334, 351
AciI	CCGC	6 cut(s)	5, 114, 604, 1450, 1999, 2076
AclWI	GGATC	8 cut(s)	1040, 2153, 2166, 2529, 2813, 2826, 2996, 3014
AcoI	YGGCCR	2 cut(s)	622, 765
AcsI	RAATTY	5 cut(s)	805, 1279, 1343, 1563, 1709
AcuI	CTGAAG	4 cut(s)	408, 1411, 1745, 3009
AcyI	GRCGYC	1 cut(s)	11
AdeI	CACNNNGTG	1 cut(s)	1631
AfaI	GTAC	3 cut(s)	1500, 1510, 2630
AfiI	CCNNNNNNNGG	6 cut(s)	94, 405, 1975, 1998, 2230, 2752
AflII	CTTAAG	2 cut(s)	2182, 2771
AgeI	ACCGGT	1 cut(s)	2917
AhdI	GACNNNNNGTC	2 cut(s)	1491, 1537
AhlI	ACTAGT	1 cut(s)	1514
AjnI	CCWGG	4 cut(s)	242, 624, 2161, 2692
AjuI	GAANNNNNNNTTGG	2 cut(s)	1269, 1301
AleI	CACNNNNGTG	1 cut(s)	49
AloI	GAACNNNNNNTCC	6 cut(s)	552, 584, 1125, 1157, 2587, 2619
Alw21I	GWGCWC	3 cut(s)	56, 2760, 3014
Alw26I	GTCTC	3 cut(s)	2214, 2379, 2934
AlwI	GGATC	8 cut(s)	1040, 2153, 2166, 2529, 2813, 2826, 2996, 3014
AlwNI	CAGNNNCTG	2 cut(s)	1331, 3032
Ama87I	CYCGRG	1 cut(s)	1050
AoxI	GGCC	5 cut(s)	430, 622, 655, 765, 1966
ApaI	GGGCCC	1 cut(s)	1970
ApeKI	GCWGC	7 cut(s)	91, 247, 917, 929, 1325, 3029, 3032
ApoI	RAATTY	5 cut(s)	805, 1279, 1343, 1563, 1709
ArsI	GACNNNNNNTTYG	4 cut(s)	1621, 1653, 2480, 2512
AseI	ATTAAT	1 cut(s)	554
AsiGI	ACCGGT	1 cut(s)	2917
Asp700I	GAANNNNTTC	2 cut(s)	1020, 1101
AspLEI	GCGC	5 cut(s)	13, 304, 336, 353, 1188
AspS9I	GGNCC	5 cut(s)	881, 1966, 1967, 1978, 2260
AsuHPI	GGTGA	5 cut(s)	36, 37, 245, 1196, 2932
AsuII	TTCGAA	1 cut(s)	416
AvaI	CYCGRG	1 cut(s)	1050
AvaII	GGWCC	3 cut(s)	881, 1978, 2260
BaeGI	GKGCMC	1 cut(s)	1970
BalI	TGGCCA	2 cut(s)	624, 767
BamHI	GGATCC	2 cut(s)	2158, 2818
BanI	GGYRCC	1 cut(s)	10
BanII	GRGCYC	3 cut(s)	1970, 2416, 3014
BbsI	GAAGAC	6 cut(s)	169, 335, 1417, 2199, 2413, 2630
Bbv12I	GWGCWC	3 cut(s)	56, 2760, 3014
BbvCI	CCTCAGC	4 cut(s)	320, 1872, 1974, 2591
BbvI	GCAGC	7 cut(s)	103, 259, 904, 916, 1337, 3019, 3041
BccI	CCATC	5 cut(s)	848, 1190, 2863, 2917, 2930
BceAI	ACGGC	2 cut(s)	383, 617
BcgI	CGANNNNNNTGC	2 cut(s)	168, 202
BciT130I	CCWGG	4 cut(s)	244, 626, 2163, 2694
BciVI	GTATCC	1 cut(s)	2798
BcoDI	GTCTC	3 cut(s)	2214, 2379, 2934
BcuI	ACTAGT	1 cut(s)	1514
BfaI	CTAG	8 cut(s)	897, 1473, 1515, 1640, 2012, 2297, 2723, 3048
BfmI	CTRYAG	3 cut(s)	885, 930, 1323
BfoI	RGCGCY	2 cut(s)	14, 1189
BfrI	CTTAAG	2 cut(s)	2182, 2771
BfuAI	ACCTGC	3 cut(s)	892, 1661, 2271
BfuI	GTATCC	1 cut(s)	2798
BglII	AGATCT	1 cut(s)	1036
BisI	GCNGC	7 cut(s)	92, 248, 918, 930, 1326, 3030, 3033
BlsI	GCNGC	7 cut(s)	93, 249, 919, 931, 1327, 3031, 3034
Bme1390I	CCNGG	4 cut(s)	244, 626, 2163, 2694
Bme18I	GGWCC	3 cut(s)	881, 1978, 2260
BmeRI	GACNNNNNGTC	2 cut(s)	1491, 1537
BmeT110I	CYCGRG	1 cut(s)	1050
BmgT120I	GGNCC	5 cut(s)	881, 1966, 1967, 1978, 2260
BmiI	GGNNCC	7 cut(s)	12, 100, 630, 1968, 2160, 2720, 2820
BmrFI	CCNGG	4 cut(s)	244, 626, 2163, 2694
BmsI	GCATC	6 cut(s)	1073, 1728, 1734, 2271, 2491, 2698
BpiI	GAAGAC	6 cut(s)	169, 335, 1417, 2199, 2413, 2630
BpmI	CTGGAG	3 cut(s)	525, 1210, 1274
Bpu10I	CCTNAGC	4 cut(s)	320, 1872, 1974, 2591
Bpu14I	TTCGAA	1 cut(s)	416
BpuEI	CTTGAG	3 cut(s)	772, 820, 2546
Bsa29I	ATCGAT	1 cut(s)	2121
BsaAI	YACGTR	1 cut(s)	2801
BsaHI	GRCGYC	1 cut(s)	11
BsaI	GGTCTC	3 cut(s)	2214, 2379, 2934
BsaJI	CCNNGG	4 cut(s)	640, 768, 1129, 1693
BsaWI	WCCGGW	2 cut(s)	2581, 2917
BsaXI	ACNNNNNCTCC	4 cut(s)	558, 588, 2587, 2617
Bsc4I	CCNNNNNNNGG	6 cut(s)	94, 405, 1975, 1998, 2230, 2752
Bse118I	RCCGGY	2 cut(s)	87, 2917
Bse1I	ACTGG	7 cut(s)	235, 508, 1263, 1291, 1321, 2149, 2908
BseBI	CCWGG	4 cut(s)	244, 626, 2163, 2694
BseCI	ATCGAT	1 cut(s)	2121
BseDI	CCNNGG	4 cut(s)	640, 768, 1129, 1693
BseGI	GGATG	9 cut(s)	840, 970, 1125, 1317, 1414, 1482, 2476, 2713, 2941
BseLI	CCNNNNNNNGG	6 cut(s)	94, 405, 1975, 1998, 2230, 2752
BseMII	CTCAG	6 cut(s)	305, 311, 1533, 1863, 1965, 2582
BseNI	ACTGG	7 cut(s)	235, 508, 1263, 1291, 1321, 2149, 2908
BseRI	GAGGAG	4 cut(s)	803, 1952, 2608, 2998
BseSI	GKGCMC	1 cut(s)	1970
BseXI	GCAGC	7 cut(s)	103, 259, 904, 916, 1337, 3019, 3041
BseYI	CCCAGC	1 cut(s)	94
Bsh1236I	CGCG	2 cut(s)	334, 351
Bsh1285I	CGRYCG	1 cut(s)	904
BshFI	GGCC	5 cut(s)	432, 624, 657, 767, 1968
BshNI	GGYRCC	1 cut(s)	10
BshTI	ACCGGT	1 cut(s)	2917
BshVI	ATCGAT	1 cut(s)	2121
BsiEI	CGRYCG	1 cut(s)	904
BsiHKAI	GWGCWC	3 cut(s)	56, 2760, 3014
BsiHKCI	CYCGRG	1 cut(s)	1050
BsiSI	CCGG	3 cut(s)	88, 2582, 2918
BslFI	GGGAC	2 cut(s)	423, 2107
BslI	CCNNNNNNNGG	6 cut(s)	94, 405, 1975, 1998, 2230, 2752
BsmAI	GTCTC	3 cut(s)	2214, 2379, 2934
BsmFI	GGGAC	2 cut(s)	423, 2107
BsmI	GAATGC	3 cut(s)	177, 1084, 1708
BsnI	GGCC	5 cut(s)	432, 624, 657, 767, 1968
Bso31I	GGTCTC	3 cut(s)	2214, 2379, 2934
BsoBI	CYCGRG	1 cut(s)	1050
Bsp119I	TTCGAA	1 cut(s)	416
Bsp120I	GGGCCC	1 cut(s)	1966
Bsp1286I	GDGCHC	5 cut(s)	56, 1970, 2416, 2760, 3014
Bsp143I	GATC	9 cut(s)	901, 1036, 1045, 2158, 2521, 2740, 2818, 3001, 3019
BspACI	CCGC	6 cut(s)	5, 114, 604, 1450, 1999, 2076
BspANI	GGCC	5 cut(s)	432, 624, 657, 767, 1968
BspCNI	CTCAG	6 cut(s)	304, 312, 1534, 1864, 1966, 2583
BspDI	ATCGAT	1 cut(s)	2121
BspFNI	CGCG	2 cut(s)	334, 351
BspLI	GGNNCC	7 cut(s)	12, 100, 630, 1968, 2160, 2720, 2820
BspMAI	CTGCAG	3 cut(s)	889, 934, 1327
BspMI	ACCTGC	3 cut(s)	892, 1661, 2271
BspPI	GGATC	8 cut(s)	1040, 2153, 2166, 2529, 2813, 2826, 2996, 3014
BspT104I	TTCGAA	1 cut(s)	416
BspT107I	GGYRCC	1 cut(s)	10
BspTI	CTTAAG	2 cut(s)	2182, 2771
BspTNI	GGTCTC	3 cut(s)	2214, 2379, 2934
BsrFI	RCCGGY	2 cut(s)	87, 2917
BsrI	ACTGG	7 cut(s)	235, 508, 1263, 1291, 1321, 2149, 2908
BssAI	RCCGGY	2 cut(s)	87, 2917
BssECI	CCNNGG	4 cut(s)	640, 768, 1129, 1693
BssMI	GATC	9 cut(s)	901, 1036, 1045, 2158, 2521, 2740, 2818, 3001, 3019
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	2 cut(s)	640, 1693
Bst2UI	CCWGG	4 cut(s)	244, 626, 2163, 2694
Bst6I	CTCTTC	3 cut(s)	21, 413, 1011
BstACI	GRCGYC	1 cut(s)	11
BstAFI	CTTAAG	2 cut(s)	2182, 2771
BstBAI	YACGTR	1 cut(s)	2801
BstBI	TTCGAA	1 cut(s)	416
BstC8I	GCNNGC	3 cut(s)	177, 1452, 2078
BstDEI	CTNAG	8 cut(s)	291, 320, 1106, 1542, 1872, 1974, 2591, 2860
BstDSI	CCRYGG	2 cut(s)	768, 1129
BstENI	CCTNNNNNAGG	1 cut(s)	2750
BstF5I	GGATG	9 cut(s)	840, 970, 1125, 1317, 1414, 1482, 2476, 2713, 2941
BstFNI	CGCG	2 cut(s)	334, 351
BstH2I	RGCGCY	2 cut(s)	14, 1189
BstHHI	GCGC	5 cut(s)	13, 304, 336, 353, 1188
BstKTI	GATC	9 cut(s)	904, 1039, 1048, 2161, 2524, 2743, 2821, 3004, 3022
BstMAI	GTCTC	3 cut(s)	2214, 2379, 2934
BstMBI	GATC	9 cut(s)	901, 1036, 1045, 2158, 2521, 2740, 2818, 3001, 3019
BstMCI	CGRYCG	1 cut(s)	904
BstMWI	GCNNNNNNNGC	5 cut(s)	926, 1088, 1183, 1217, 1877
BstNI	CCWGG	4 cut(s)	244, 626, 2163, 2694
BstSCI	CCNGG	4 cut(s)	242, 624, 2161, 2692
BstSFI	CTRYAG	3 cut(s)	885, 930, 1323
BstSLI	GKGCMC	1 cut(s)	1970
BstUI	CGCG	2 cut(s)	334, 351
BstV1I	GCAGC	7 cut(s)	103, 259, 904, 916, 1337, 3019, 3041
BstV2I	GAAGAC	6 cut(s)	169, 335, 1417, 2199, 2413, 2630
BstX2I	RGATCY	4 cut(s)	1036, 2158, 2521, 2818
BstYI	RGATCY	4 cut(s)	1036, 2158, 2521, 2818
Bsu15I	ATCGAT	1 cut(s)	2121
BsuI	GTATCC	1 cut(s)	2798
BsuRI	GGCC	5 cut(s)	432, 624, 657, 767, 1968
BsuTUI	ATCGAT	1 cut(s)	2121
BtgI	CCRYGG	2 cut(s)	768, 1129
BtsCI	GGATG	9 cut(s)	840, 970, 1125, 1317, 1414, 1482, 2476, 2713, 2941
BtsI	GCAGTG	3 cut(s)	186, 1320, 1653
BtsIMutI	CAGTG	9 cut(s)	186, 228, 736, 957, 1064, 1256, 1320, 1653, 1791
BveI	ACCTGC	3 cut(s)	892, 1661, 2271
Cac8I	GCNNGC	3 cut(s)	177, 1452, 2078
CaiI	CAGNNNCTG	2 cut(s)	1331, 3032
CfoI	GCGC	5 cut(s)	13, 304, 336, 353, 1188
Cfr10I	RCCGGY	2 cut(s)	87, 2917
Cfr13I	GGNCC	5 cut(s)	881, 1966, 1967, 1978, 2260
ClaI	ATCGAT	1 cut(s)	2121
CseI	GACGC	1 cut(s)	340
Csp6I	GTAC	3 cut(s)	1499, 1509, 2629
CspAI	ACCGGT	1 cut(s)	2917
CviAII	CATG	7 cut(s)	466, 572, 1571, 1812, 1839, 2084, 2105
CviQI	GTAC	3 cut(s)	1499, 1509, 2629
DdeI	CTNAG	8 cut(s)	291, 320, 1106, 1542, 1872, 1974, 2591, 2860
DinI	GGCGCC	1 cut(s)	12
DpnI	GATC	9 cut(s)	903, 1038, 1047, 2160, 2523, 2742, 2820, 3003, 3021
DpnII	GATC	9 cut(s)	901, 1036, 1045, 2158, 2521, 2740, 2818, 3001, 3019
DraI	TTTAAA	1 cut(s)	546
DraIII	CACNNNGTG	1 cut(s)	1631
DriI	GACNNNNNGTC	2 cut(s)	1491, 1537
EaeI	YGGCCR	2 cut(s)	622, 765
Eam1104I	CTCTTC	3 cut(s)	21, 413, 1011
Eam1105I	GACNNNNNGTC	2 cut(s)	1491, 1537
EarI	CTCTTC	3 cut(s)	21, 413, 1011
EciI	GGCGGA	1 cut(s)	129
Ecl136II	GAGCTC	1 cut(s)	3012
Eco130I	CCWWGG	2 cut(s)	640, 1693
Eco147I	AGGCCT	1 cut(s)	432
Eco24I	GRGCYC	3 cut(s)	1970, 2416, 3014
Eco31I	GGTCTC	3 cut(s)	2214, 2379, 2934
Eco32I	GATATC	2 cut(s)	2338, 2476
Eco47I	GGWCC	3 cut(s)	881, 1978, 2260
Eco53kI	GAGCTC	1 cut(s)	3012
Eco57I	CTGAAG	4 cut(s)	408, 1411, 1745, 3009
Eco88I	CYCGRG	1 cut(s)	1050
EcoICRI	GAGCTC	1 cut(s)	3012
EcoNI	CCTNNNNNAGG	1 cut(s)	2750
EcoO109I	RGGNCCY	3 cut(s)	1966, 1967, 2260
EcoRI	GAATTC	1 cut(s)	1563
EcoRII	CCWGG	4 cut(s)	242, 624, 2161, 2692
EcoRV	GATATC	2 cut(s)	2338, 2476
EcoT14I	CCWWGG	2 cut(s)	640, 1693
EcoT22I	ATGCAT	1 cut(s)	553
EcoT38I	GRGCYC	3 cut(s)	1970, 2416, 3014
EgeI	GGCGCC	1 cut(s)	12
EheI	GGCGCC	1 cut(s)	12
ErhI	CCWWGG	2 cut(s)	640, 1693
FaeI	CATG	7 cut(s)	469, 575, 1574, 1815, 1842, 2087, 2108
FalI	AAGNNNNNCTT	6 cut(s)	397, 429, 1128, 1160, 2744, 2776
FaqI	GGGAC	2 cut(s)	423, 2107
FatI	CATG	7 cut(s)	465, 571, 1570, 1811, 1838, 2083, 2104
FauI	CCCGC	3 cut(s)	597, 1443, 2069
FauNDI	CATATG	1 cut(s)	718
FblI	GTMKAC	1 cut(s)	2037
Fnu4HI	GCNGC	7 cut(s)	92, 248, 918, 930, 1326, 3030, 3033
FokI	GGATG	9 cut(s)	827, 977, 1112, 1324, 1421, 1489, 2483, 2720, 2948
FriOI	GRGCYC	3 cut(s)	1970, 2416, 3014
Fsp4HI	GCNGC	7 cut(s)	92, 248, 918, 930, 1326, 3030, 3033
FspBI	CTAG	8 cut(s)	897, 1473, 1515, 1640, 2012, 2297, 2723, 3048
GlaI	GCGC	5 cut(s)	12, 303, 335, 352, 1187
GluI	GCNGC	7 cut(s)	92, 248, 918, 930, 1326, 3030, 3033
GsaI	CCCAGC	1 cut(s)	98
GsuI	CTGGAG	3 cut(s)	525, 1210, 1274
HaeII	RGCGCY	2 cut(s)	14, 1189
HaeIII	GGCC	5 cut(s)	432, 624, 657, 767, 1968
HapII	CCGG	3 cut(s)	88, 2582, 2918
HgaI	GACGC	1 cut(s)	340
HhaI	GCGC	5 cut(s)	13, 304, 336, 353, 1188
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	7 cut(s)	469, 575, 1574, 1815, 1842, 2087, 2108
Hin6I	GCGC	5 cut(s)	11, 302, 334, 351, 1186
HinP1I	GCGC	5 cut(s)	11, 302, 334, 351, 1186
HincII	GTYRAC	1 cut(s)	586
HindII	GTYRAC	1 cut(s)	586
HindIII	AAGCTT	2 cut(s)	1361, 2837
HpaII	CCGG	3 cut(s)	88, 2582, 2918
HphI	GGTGA	5 cut(s)	36, 37, 245, 1196, 2932
Hpy166II	GTNNAC	6 cut(s)	240, 586, 1117, 1161, 2038, 2798
Hpy188III	TCNNGA	9 cut(s)	144, 344, 799, 879, 2012, 2189, 2525, 2539, 2648
Hpy8I	GTNNAC	6 cut(s)	240, 586, 1117, 1161, 2038, 2798
Hpy99I	CGWCG	2 cut(s)	404, 2118
HpyCH4IV	ACGT	2 cut(s)	1901, 2800
HpyF10VI	GCNNNNNNNGC	5 cut(s)	926, 1088, 1183, 1217, 1877
HpyF3I	CTNAG	8 cut(s)	291, 320, 1106, 1542, 1872, 1974, 2591, 2860
HpySE526I	ACGT	2 cut(s)	1901, 2800
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	7 cut(s)	469, 575, 1574, 1815, 1842, 2087, 2108
HspAI	GCGC	5 cut(s)	11, 302, 334, 351, 1186
KasI	GGCGCC	1 cut(s)	10
Kzo9I	GATC	9 cut(s)	901, 1036, 1045, 2158, 2521, 2740, 2818, 3001, 3019
LmnI	GCTCC	7 cut(s)	59, 262, 316, 790, 1939, 2595, 2755
Lsp1109I	GCAGC	7 cut(s)	103, 259, 904, 916, 1337, 3019, 3041
LweI	GCATC	6 cut(s)	1073, 1728, 1734, 2271, 2491, 2698
MaeI	CTAG	8 cut(s)	897, 1473, 1515, 1640, 2012, 2297, 2723, 3048
MaeII	ACGT	2 cut(s)	1901, 2800
MaeIII	GTNAC	8 cut(s)	42, 577, 721, 1252, 1369, 1529, 1648, 2968
MalI	GATC	9 cut(s)	903, 1038, 1047, 2160, 2523, 2742, 2820, 3003, 3021
MboI	GATC	9 cut(s)	901, 1036, 1045, 2158, 2521, 2740, 2818, 3001, 3019
MfeI	CAATTG	4 cut(s)	1374, 1547, 2268, 2599
MflI	RGATCY	4 cut(s)	1036, 2158, 2521, 2818
MhlI	GDGCHC	5 cut(s)	56, 1970, 2416, 2760, 3014
MlsI	TGGCCA	2 cut(s)	624, 767
MluNI	TGGCCA	2 cut(s)	624, 767
Mly113I	GGCGCC	1 cut(s)	11
MlyI	GAGTC	3 cut(s)	245, 1526, 2282
MmeI	TCCRAC	4 cut(s)	475, 688, 1406, 1540
Mox20I	TGGCCA	2 cut(s)	624, 767
Mph1103I	ATGCAT	1 cut(s)	553
MroXI	GAANNNNTTC	2 cut(s)	1020, 1101
MscI	TGGCCA	2 cut(s)	624, 767
MslI	CAYNNNNRTG	1 cut(s)	49
Msp20I	TGGCCA	2 cut(s)	624, 767
MspA1I	CMGCKG	3 cut(s)	40, 94, 3032
MspCI	CTTAAG	2 cut(s)	2182, 2771
MspI	CCGG	3 cut(s)	88, 2582, 2918
MspR9I	CCNGG	4 cut(s)	244, 626, 2163, 2694
MunI	CAATTG	4 cut(s)	1374, 1547, 2268, 2599
Mva1269I	GAATGC	3 cut(s)	177, 1084, 1708
MvaI	CCWGG	4 cut(s)	244, 626, 2163, 2694
MvnI	CGCG	2 cut(s)	334, 351
MwoI	GCNNNNNNNGC	5 cut(s)	926, 1088, 1183, 1217, 1877
NarI	GGCGCC	1 cut(s)	11
NdeI	CATATG	1 cut(s)	718
NdeII	GATC	9 cut(s)	901, 1036, 1045, 2158, 2521, 2740, 2818, 3001, 3019
NlaIII	CATG	7 cut(s)	469, 575, 1574, 1815, 1842, 2087, 2108
NlaIV	GGNNCC	7 cut(s)	12, 100, 630, 1968, 2160, 2720, 2820
NmuCI	GTSAC	5 cut(s)	42, 1252, 1529, 1648, 2968
NsiI	ATGCAT	1 cut(s)	553
NspV	TTCGAA	1 cut(s)	416
OliI	CACNNNNGTG	1 cut(s)	49
PceI	AGGCCT	1 cut(s)	432
PctI	GAATGC	3 cut(s)	177, 1084, 1708
PdmI	GAANNNNTTC	2 cut(s)	1020, 1101
PflFI	GACNNNGTC	2 cut(s)	1982, 2390
PfoI	TCCNGGA	1 cut(s)	2161
PinAI	ACCGGT	1 cut(s)	2917
PkrI	GCNGC	7 cut(s)	93, 249, 919, 931, 1327, 3031, 3034
Ple19I	CGATCG	1 cut(s)	904
PleI	GAGTC	3 cut(s)	244, 1526, 2281
PluTI	GGCGCC	1 cut(s)	14
PpsI	GAGTC	3 cut(s)	244, 1526, 2281
Ppu21I	YACGTR	1 cut(s)	2801
PpuMI	RGGWCCY	1 cut(s)	2260
PshBI	ATTAAT	1 cut(s)	554
PsiI	TTATAA	1 cut(s)	912
Psp124BI	GAGCTC	1 cut(s)	3014
Psp5II	RGGWCCY	1 cut(s)	2260
Psp6I	CCWGG	4 cut(s)	242, 624, 2161, 2692
PspFI	CCCAGC	1 cut(s)	94
PspGI	CCWGG	4 cut(s)	242, 624, 2161, 2692
PspN4I	GGNNCC	7 cut(s)	12, 100, 630, 1968, 2160, 2720, 2820
PspOMI	GGGCCC	1 cut(s)	1966
PspPI	GGNCC	5 cut(s)	881, 1966, 1967, 1978, 2260
PspPPI	RGGWCCY	1 cut(s)	2260
PstI	CTGCAG	3 cut(s)	889, 934, 1327
PstNI	CAGNNNCTG	2 cut(s)	1331, 3032
PsuI	RGATCY	4 cut(s)	1036, 2158, 2521, 2818
PsyI	GACNNNGTC	2 cut(s)	1982, 2390
PvuI	CGATCG	1 cut(s)	904
PvuII	CAGCTG	3 cut(s)	40, 94, 3032
RsaI	GTAC	3 cut(s)	1500, 1510, 2630
RsaNI	GTAC	3 cut(s)	1499, 1509, 2629
RseI	CAYNNNNRTG	1 cut(s)	49
SacI	GAGCTC	1 cut(s)	3014
SatI	GCNGC	7 cut(s)	92, 248, 918, 930, 1326, 3030, 3033
Sau3AI	GATC	9 cut(s)	901, 1036, 1045, 2158, 2521, 2740, 2818, 3001, 3019
Sau96I	GGNCC	5 cut(s)	881, 1966, 1967, 1978, 2260
SchI	GAGTC	3 cut(s)	245, 1526, 2282
ScrFI	CCNGG	4 cut(s)	244, 626, 2163, 2694
SduI	GDGCHC	5 cut(s)	56, 1970, 2416, 2760, 3014
SfaNI	GCATC	6 cut(s)	1073, 1728, 1734, 2271, 2491, 2698
SfcI	CTRYAG	3 cut(s)	885, 930, 1323
SfoI	GGCGCC	1 cut(s)	12
SfuI	TTCGAA	1 cut(s)	416
SinI	GGWCC	3 cut(s)	881, 1978, 2260
SmiI	ATTTAAAT	1 cut(s)	546
SmiMI	CAYNNNNRTG	1 cut(s)	49
SmlI	CTYRAG	5 cut(s)	751, 799, 2182, 2525, 2771
SmoI	CTYRAG	5 cut(s)	751, 799, 2182, 2525, 2771
SpeI	ACTAGT	1 cut(s)	1514
SseBI	AGGCCT	1 cut(s)	432
SsiI	CCGC	6 cut(s)	5, 114, 604, 1450, 1999, 2076
SspDI	GGCGCC	1 cut(s)	10
SspI	AATATT	3 cut(s)	2491, 2555, 2737
SspMI	CTAG	8 cut(s)	897, 1473, 1515, 1640, 2012, 2297, 2723, 3048
SstI	GAGCTC	1 cut(s)	3014
StuI	AGGCCT	1 cut(s)	432
StyD4I	CCNGG	4 cut(s)	242, 624, 2161, 2692
StyI	CCWWGG	2 cut(s)	640, 1693
SwaI	ATTTAAAT	1 cut(s)	546
TaiI	ACGT	2 cut(s)	1904, 2803
TaqI	TCGA	8 cut(s)	143, 416, 1168, 1675, 2116, 2121, 2140, 3018
TatI	WGTACW	1 cut(s)	1498
TscAI	CASTG	9 cut(s)	186, 235, 736, 964, 1064, 1263, 1327, 1653, 1791
TseFI	GTSAC	5 cut(s)	42, 1252, 1529, 1648, 2968
TseI	GCWGC	7 cut(s)	91, 247, 917, 929, 1325, 3029, 3032
Tsp45I	GTSAC	5 cut(s)	42, 1252, 1529, 1648, 2968
TspGWI	ACGGA	5 cut(s)	785, 1118, 2326, 2414, 2994
TspRI	CASTG	9 cut(s)	186, 235, 736, 964, 1064, 1263, 1327, 1653, 1791
Tth111I	GACNNNGTC	2 cut(s)	1982, 2390
Vha464I	CTTAAG	2 cut(s)	2182, 2771
VpaK11BI	GGWCC	3 cut(s)	881, 1978, 2260
VspI	ATTAAT	1 cut(s)	554
XagI	CCTNNNNNAGG	1 cut(s)	2750
XapI	RAATTY	5 cut(s)	805, 1279, 1343, 1563, 1709
XbaI	TCTAGA	1 cut(s)	2011
XcmI	CCANNNNNNNNNTGG	2 cut(s)	458, 1809
XmiI	GTMKAC	1 cut(s)	2037
XmnI	GAANNNNTTC	2 cut(s)	1020, 1101
XspI	CTAG	8 cut(s)	897, 1473, 1515, 1640, 2012, 2297, 2723, 3048
Zsp2I	ATGCAT	1 cut(s)	553

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.