Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0005400

Physical Location & Seq

Genomic Coordinates Reverse (-)

30717974 .. 30724490

Gene Structure 6517 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G024110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3075 bp

ATGGCTCTGGTGAAGCCCCATTCCCTCTCACCTCTCTCTTCCAACTCCCGCCATGCCCTCCCCCTCAACAACTCCCATTTCCGCACTTCTTTCTTCGCCGGAAAACCCTTCCGACTGCGGGAAACCACACGGATTTCGAGCCGGAGACGCCGGCGAGTGCAGGCAGTGGCTTCCCTCGGAGGTTTGCTGGGCGGGATTTTCAAAGGGACTGATACCGGTGAGTCCACCAGGCAGCAGTACGCGCAGACTGTGTCTCTGATCAACGGCTTGGAAGCTCAGATGTCTAAGCTTTCCGACTCCGAGCTCAGGGAGAAGACTCTGCAGTTTCAACAACGCGCCAAGCAGGGCGAGTCCTTGGACTCTCTTTTACCCGAAGCATTTGCTGTGATCAGAGAGGCTTCGAGGAGGGTTTTAGGCCTTCGTCCCTTTGACGTCCAACTCATAGGCGGCATAGTTCTTCACAAGGGGGAAATAGCTGAAATGAGGACCGGAGAAGGAAAGACTCTTGTTGCTATATTACCAGCTTATTTGAATGCATTAACTGGAAAGGGAGTTCATGTTGTCACCGTCAATGATTATCTGGCCCGACGTGATTGTGAGTGGGTTGGTCAAGTTCCTCGTTTCCTTGGATTGAAGGTTGGCCTAATCCAACGTATAAATATGACAAGTGAACAAAGAAGGGAGAACTACTTATGTGACATCACATATGTCACAAACAGCGAGCTTGGTTTTGATTTCCTGAGAGATAATCTGGCCACGGAAAGTAATTCTTGCTTTATTTTCTTTACTAATAGTGTTGAGGAGCTTGTCTTGAGGAATTTCAATTACTGTGTTATTGATGAGGTTGATTCCATCCTTATTGATGAAGCAAGAACACCTCTCATTATATCTGGACCTGCAGAAAAACCTAGTGATAGGTATTATAAAGCTGCAAAGATGGCTTCAGTGTTTGAGCGAGATATACATTATACTGTGGATGAAAAGCAGAAAACAGTTCTGCTTTCAGAACAAGGATATGAAGATGCTGAAGAGATTCTAGGTGTCAAAGATTTATATGATCCCCGCGAACAGTGGGCATCTTATGTTCTGAATGCCGTGAAAGCAAAAGAACTGTTTCTTAGAGATGTAAATTATATAATTCGTGGAAAAGAGGTTCTTATCGTTGATGAGTTTACAGGACGAGTTATGCAGGGGAGGCGGTGGAGTGATGGACTTCACCAAGCAGTTGAAGCAAAAGAAGGTCTGCCGATTCAAAACGAAACTGTAACTCTGGCATCTATTAGCTACCAAAACTTCTTTCTCCAGTTTCCAAAACTTTGTGGAATGACTGGAACTGCAGCAACTGAAAGCACAGAATTTGAAAGCATATACAAGCTTAAAGTTACAATTGTCCCCACAAACAAGCCTATGATACGAAAGGATGAGTCTGATGTAGTTTTCAGGGCAGCTACAGGTAAATGGCGGGCAGTTGTAGTAGAGATTTCTAGAATGCACAAAACAGGTCGCCCTGTACTTGTTGGCACAACTAGTGTTGAGCAGAGTGACTCATTGTCTGAGCAGTTGCAAGAAGTTGGCATTCCCCATGAGGTTCTCAACGCAAAACCAGAGAATGTGGAGAGGGAAGCAGAAATTGTGGCACAAAGCGGTCGTCTAGGGGCAGTTACTATTGCAACCAATATGGCAGGTCGAGGAACAGACATAATCCTTGGTGGGAATGCAGAATTTATGGCAAGGTTGAAGCTGCGTGAGATGCTTATGCCAAGAGTTGTAAAACTAACTGAAGGAGGTTATGTCTCAGTGAAAAAACTTCCGCCGAAAAAGTCCTGGAAGGTTAATGAAAAGCTATTTCCATGCAAACTGTCCAATGAAAAGACCAAGTTAGCTGAGGAAGCTGTGAATTTAGCTGTTGAAACTTGGGGTCAGAGATCATTGACGGAGCTTGAAGCAGAGGAGCGTCTTTCTTATTCTTGCGAAAAGGGACCTGCTTTGGATGAAGTGATAGCCAAACTGCGGAGTGCTTTTTTAGAAATCATGAAAGAATATAAGGGGTACACAGAGGAAGAAAGGAAGAAGGTTGTGTCTGCTGGTGGGCTTCATGTTGTCGGGACAGAACGCCATGAATCTCGCCGAGTTGATAATCAGTTACGTGGTCGAACTGGACGGCAGGGGGATCCTGGAAGTTCACGCTTCTTCTTAAGTCTTGAAGATAACATCTTTCGTATATTTGGTGGAGATCGTATTCAGGGTTTAATGAGAGCTTTTAGAGTCGAGGACTTGCCAATTGAGTCCAAGATGCTGACCAAAGCACTAGATGAAGCTCAGCGGAAAGTGGAAAACTATTTTTTCGATATTCGAAAGCAGTTGTTTGAATTTGATGAAGTCCTAAACAGCCAAAGAGACCGTGTGTATATGGAAAGAAGGAGAGCCCTTGAATCTGACAATCTGCAATCACTTATAATTGAATATGCTGAACTAACAATGGATGATATCTTAGAGGCAAATATTGGTTCAGAGTCTTCAAAAGAAAGCTGGGATCTTGATAAGCTCATCAAGAAACTTCAACAATATTGCTATCTATTGAATGATTTGACGCCGGATGTGCTGAGGAGCGAATGTTCAAGTTATGAAGATTTGCAGGACTACCTCCGTCTTCGAGGTCGTGAAGCATATTTGCAGAAAAGGACTATTATTGAGAGCCAGGCACCGGGCTTGATGAAGGATGCTGAACGGTTCTTAGTTTTGAACAATATTGACCGATTGTGGAAGGAGCACTTACAAGCGCTCAAGTTCGTTCAGCAAGCTGTTGGTTTGCGTGGGTACGCACAACGGGATCCACTTATTGAATATAAGCTTGAAGGCTACAATCTTTTCTTGGACATGATGGCACAGATAAGAAGAAATGTTATATATTCCATATACCAGTTTCAACCTGTGATGGTAAAGAAGGATGGGGACCAAAGAGAGAATAAGAAATCAGGAAAACTTGTCACTAATGGTAGCGGTAATGGTAACCCCAATTCAGTTGGCGGTGTTGAGTCTTCTTCAGCAGCTGCCACCCCCCAATCTAGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071806 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

1024

Amino Acids

115.92

Weight (kDa)

7.54

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	74 - 454	8.8e-121	SecA DEAD-like domain
DEAD	PF00270	158 - 287	6.4e-06	DEAD/DEAH box helicase
SecA_PP_bind	PF01043	304 - 410	1e-37	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	469 - 758	1.6e-82	SecA P-loop domain
SecA_SW	PF07516	760 - 976	8.2e-56	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01800 AT4G01800 AT4G01800
fragaria_vesca	FvH4_1g26240
malus_domestica	MD17G1285000.v1.1
prunus_persica	Prupe.3G002700_v2.0.a1
pyrus_communis	pycom09g19850 pycom17g28080 pycom17g28090
rosa_chinensis	RchiOBHm_Chr3g0485781
rosa_laevigata	RLG00000023125
rosa_multiflora	Rmu_co8471925.1_g000001 Rmu_sc0012870.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123840
rosa_rugosa	Rorug03G0221400
rosa_samantha	Rh1AG180100 Rh1CG167100 Rh3AG270700 Rh3BG306200 Rh3BG306300 Rh3CG304600 Rh3DG300700 Rh4AG241300 Rh4AG241400 Rh4BG244600 Rh4BG244700 Rh4CG255400 Rh7CG119700
rosa_wichuraiana	Rw3G024110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	924, 2456
AatII	GACGTC	1 cut(s)	435
Acc36I	ACCTGC	3 cut(s)	904, 1673, 1990
AccB1I	GGYRCC	1 cut(s)	2701
AccII	CGCG	3 cut(s)	242, 336, 1065
AclWI	GGATC	6 cut(s)	1052, 2165, 2178, 2541, 2825, 2838
AcoI	YGGCCR	1 cut(s)	753
AcsI	RAATTY	5 cut(s)	817, 1355, 1721, 1897, 2369
AcuI	CTGAAG	4 cut(s)	927, 1047, 1800, 3027
AcyI	GRCGYC	3 cut(s)	148, 432, 2591
AfaI	GTAC	4 cut(s)	239, 1512, 2051, 2819
AfeI	AGCGCT	1 cut(s)	2781
AfiI	CCNNNNNNNGG	4 cut(s)	118, 306, 2010, 2704
AflII	CTTAAG	1 cut(s)	2194
AgeI	ACCGGT	1 cut(s)	215
AhdI	GACNNNNNGTC	1 cut(s)	1549
AhlI	ACTAGT	1 cut(s)	1526
AjiI	CACGTC	1 cut(s)	590
AjnI	CCWGG	4 cut(s)	227, 1823, 2173, 2697
AjuI	GAANNNNNNNTTGG	2 cut(s)	1281, 1313
AloI	GAACNNNNNNTCC	6 cut(s)	537, 569, 1137, 1169, 2599, 2631
Alw21I	GWGCWC	2 cut(s)	306, 2772
Alw26I	GTCTC	4 cut(s)	139, 258, 1798, 2391
AlwI	GGATC	6 cut(s)	1052, 2165, 2178, 2541, 2825, 2838
AlwNI	CAGNNNCTG	2 cut(s)	1343, 3050
Aor51HI	AGCGCT	1 cut(s)	2781
AoxI	GGCC	4 cut(s)	415, 582, 640, 753
ApeKI	GCWGC	7 cut(s)	232, 929, 1337, 1445, 1741, 3047, 3050
ApoI	RAATTY	5 cut(s)	817, 1355, 1721, 1897, 2369
ArsI	GACNNNNNNTTYG	2 cut(s)	1633, 1665
AsiGI	ACCGGT	1 cut(s)	215
Asp700I	GAANNNNTTC	2 cut(s)	1032, 1113
AspLEI	GCGC	3 cut(s)	244, 338, 2782
AspS9I	GGNCC	5 cut(s)	486, 583, 893, 1979, 2953
AsuC2I	CCSGG	1 cut(s)	2706
AsuHPI	GGTGA	5 cut(s)	21, 22, 230, 556, 1208
AsuII	TTCGAA	1 cut(s)	2353
AvaII	GGWCC	4 cut(s)	486, 893, 1979, 2953
BalI	TGGCCA	1 cut(s)	755
BamHI	GGATCC	2 cut(s)	2170, 2830
BanI	GGYRCC	1 cut(s)	2701
BanII	GRGCYC	2 cut(s)	306, 2428
BbsI	GAAGAC	4 cut(s)	320, 2508, 2642, 3030
Bbv12I	GWGCWC	2 cut(s)	306, 2772
BbvCI	CCTCAGC	2 cut(s)	1884, 2603
BbvI	GCAGC	7 cut(s)	244, 916, 1349, 1457, 1728, 3037, 3059
BccI	CCATC	6 cut(s)	860, 931, 1202, 2875, 2929, 2942
BceAI	ACGGC	3 cut(s)	280, 1079, 2177
BcgI	CGANNNNNNTGC	2 cut(s)	362, 396
BciT130I	CCWGG	4 cut(s)	229, 1825, 2175, 2699
BclI	TGATCA	2 cut(s)	258, 387
BcnI	CCSGG	1 cut(s)	2706
BcoDI	GTCTC	4 cut(s)	139, 258, 1798, 2391
BcuI	ACTAGT	1 cut(s)	1526
BfaI	CTAG	7 cut(s)	909, 1037, 1485, 1527, 1652, 2309, 3066
BfmI	CTRYAG	4 cut(s)	320, 897, 1335, 1449
BfoI	RGCGCY	1 cut(s)	2783
BfrI	CTTAAG	1 cut(s)	2194
BfuAI	ACCTGC	3 cut(s)	904, 1673, 1990
BisI	GCNGC	8 cut(s)	233, 448, 930, 1338, 1446, 1742, 3048, 3051
BlpI	GCTNAGC	1 cut(s)	2319
BlsI	GCNGC	8 cut(s)	234, 449, 931, 1339, 1447, 1743, 3049, 3052
Bme1390I	CCNGG	5 cut(s)	229, 1825, 2175, 2699, 2706
Bme18I	GGWCC	4 cut(s)	486, 893, 1979, 2953
BmeRI	GACNNNNNGTC	1 cut(s)	1549
BmgBI	CACGTC	1 cut(s)	590
BmgT120I	GGNCC	5 cut(s)	486, 583, 893, 1979, 2953
BmiI	GGNNCC	5 cut(s)	1980, 2172, 2703, 2832, 2954
BmrFI	CCNGG	5 cut(s)	229, 1825, 2175, 2699, 2706
BmsI	GCATC	6 cut(s)	1012, 1085, 1283, 1740, 2283, 2710
BpiI	GAAGAC	4 cut(s)	320, 2508, 2642, 3030
BpmI	CTGGAG	1 cut(s)	1286
Bpu10I	CCTNAGC	3 cut(s)	305, 1884, 2603
Bpu1102I	GCTNAGC	1 cut(s)	2319
Bpu14I	TTCGAA	1 cut(s)	2353
BpuEI	CTTGAG	2 cut(s)	832, 2768
BpuMI	CCSGG	1 cut(s)	2706
BsaAI	YACGTR	1 cut(s)	2147
BsaHI	GRCGYC	3 cut(s)	148, 432, 2591
BsaI	GGTCTC	1 cut(s)	2391
BsaJI	CCNNGG	5 cut(s)	175, 354, 625, 756, 1705
BsaWI	WCCGGW	2 cut(s)	215, 488
BsaXI	ACNNNNNCTCC	6 cut(s)	543, 573, 1776, 1806, 2599, 2629
Bsc4I	CCNNNNNNNGG	4 cut(s)	118, 306, 2010, 2704
Bse118I	RCCGGY	2 cut(s)	150, 215
Bse1I	ACTGG	5 cut(s)	547, 1303, 1333, 2161, 2920
BseBI	CCWGG	4 cut(s)	229, 1825, 2175, 2699
BseDI	CCNNGG	5 cut(s)	175, 354, 625, 756, 1705
BseGI	GGATG	8 cut(s)	852, 982, 1426, 1996, 2488, 2602, 2725, 2953
BseLI	CCNNNNNNNGG	4 cut(s)	118, 306, 2010, 2704
BseMII	CTCAG	8 cut(s)	290, 319, 731, 1545, 1809, 1875, 2333, 2594
BseNI	ACTGG	5 cut(s)	547, 1303, 1333, 2161, 2920
BseRI	GAGGAG	4 cut(s)	418, 815, 1964, 2620
BseXI	GCAGC	7 cut(s)	244, 916, 1349, 1457, 1728, 3037, 3059
BseYI	CCCAGC	2 cut(s)	187, 2529
BsgI	GTGCAG	1 cut(s)	179
Bsh1236I	CGCG	3 cut(s)	242, 336, 1065
Bsh1285I	CGRYCG	1 cut(s)	1648
BshFI	GGCC	4 cut(s)	417, 584, 642, 755
BshNI	GGYRCC	1 cut(s)	2701
BshTI	ACCGGT	1 cut(s)	215
BsiEI	CGRYCG	1 cut(s)	1648
BsiHKAI	GWGCWC	2 cut(s)	306, 2772
BsiSI	CCGG	7 cut(s)	99, 142, 151, 216, 489, 2594, 2705
BslFI	GGGAC	6 cut(s)	220, 408, 1376, 1992, 2119, 2966
BslI	CCNNNNNNNGG	4 cut(s)	118, 306, 2010, 2704
BsmAI	GTCTC	4 cut(s)	139, 258, 1798, 2391
BsmBI	CGTCTC	1 cut(s)	139
BsmFI	GGGAC	6 cut(s)	220, 408, 1376, 1992, 2119, 2966
BsmI	GAATGC	5 cut(s)	538, 1096, 1494, 1575, 1720
BsnI	GGCC	4 cut(s)	417, 584, 642, 755
Bso31I	GGTCTC	1 cut(s)	2391
Bsp119I	TTCGAA	1 cut(s)	2353
Bsp1286I	GDGCHC	3 cut(s)	306, 2428, 2772
Bsp143I	GATC	8 cut(s)	258, 387, 1057, 1925, 2170, 2233, 2533, 2830
Bsp1720I	GCTNAGC	1 cut(s)	2319
BspANI	GGCC	4 cut(s)	417, 584, 642, 755
BspCNI	CTCAG	8 cut(s)	289, 318, 732, 1546, 1808, 1876, 2332, 2595
BspFNI	CGCG	3 cut(s)	242, 336, 1065
BspHI	TCATGA	1 cut(s)	2031
BspLI	GGNNCC	5 cut(s)	1980, 2172, 2703, 2832, 2954
BspMAI	CTGCAG	3 cut(s)	324, 901, 1339
BspMI	ACCTGC	3 cut(s)	904, 1673, 1990
BspPI	GGATC	6 cut(s)	1052, 2165, 2178, 2541, 2825, 2838
BspT104I	TTCGAA	1 cut(s)	2353
BspT107I	GGYRCC	1 cut(s)	2701
BspTI	CTTAAG	1 cut(s)	2194
BspTNI	GGTCTC	1 cut(s)	2391
BsrFI	RCCGGY	2 cut(s)	150, 215
BsrI	ACTGG	5 cut(s)	547, 1303, 1333, 2161, 2920
BssAI	RCCGGY	2 cut(s)	150, 215
BssECI	CCNNGG	5 cut(s)	175, 354, 625, 756, 1705
BssMI	GATC	8 cut(s)	258, 387, 1057, 1925, 2170, 2233, 2533, 2830
BssNI	GRCGYC	3 cut(s)	148, 432, 2591
BssT1I	CCWWGG	3 cut(s)	354, 625, 1705
Bst2UI	CCWGG	4 cut(s)	229, 1825, 2175, 2699
Bst6I	CTCTTC	2 cut(s)	43, 1023
BstACI	GRCGYC	3 cut(s)	148, 432, 2591
BstAFI	CTTAAG	1 cut(s)	2194
BstBAI	YACGTR	1 cut(s)	2147
BstBI	TTCGAA	1 cut(s)	2353
BstC8I	GCNNGC	5 cut(s)	152, 162, 722, 1464, 2799
BstDSI	CCRYGG	1 cut(s)	756
BstEII	GGTNACC	1 cut(s)	3008
BstF5I	GGATG	8 cut(s)	852, 982, 1426, 1996, 2488, 2602, 2725, 2953
BstFNI	CGCG	3 cut(s)	242, 336, 1065
BstH2I	RGCGCY	1 cut(s)	2783
BstHHI	GCGC	3 cut(s)	244, 338, 2782
BstKTI	GATC	8 cut(s)	261, 390, 1060, 1928, 2173, 2236, 2536, 2833
BstMAI	GTCTC	4 cut(s)	139, 258, 1798, 2391
BstMBI	GATC	8 cut(s)	258, 387, 1057, 1925, 2170, 2233, 2533, 2830
BstMCI	CGRYCG	1 cut(s)	1648
BstMWI	GCNNNNNNNGC	9 cut(s)	147, 241, 938, 1100, 1195, 1229, 1750, 1889, 2599
BstNI	CCWGG	4 cut(s)	229, 1825, 2175, 2699
BstPI	GGTNACC	1 cut(s)	3008
BstSCI	CCNGG	5 cut(s)	227, 1823, 2173, 2697, 2704
BstSFI	CTRYAG	4 cut(s)	320, 897, 1335, 1449
BstUI	CGCG	3 cut(s)	242, 336, 1065
BstV1I	GCAGC	7 cut(s)	244, 916, 1349, 1457, 1728, 3037, 3059
BstV2I	GAAGAC	4 cut(s)	320, 2508, 2642, 3030
BstX2I	RGATCY	3 cut(s)	2170, 2533, 2830
BstYI	RGATCY	3 cut(s)	2170, 2533, 2830
BsuRI	GGCC	4 cut(s)	417, 584, 642, 755
BtgI	CCRYGG	1 cut(s)	756
BtrI	CACGTC	1 cut(s)	590
BtsCI	GGATG	8 cut(s)	852, 982, 1426, 1996, 2488, 2602, 2725, 2953
BtsI	GCAGTG	1 cut(s)	171
BtsIMutI	CAGTG	4 cut(s)	171, 951, 1076, 1803
BveI	ACCTGC	3 cut(s)	904, 1673, 1990
Cac8I	GCNNGC	5 cut(s)	152, 162, 722, 1464, 2799
CaiI	CAGNNNCTG	2 cut(s)	1343, 3050
CciI	TCATGA	1 cut(s)	2031
CfoI	GCGC	3 cut(s)	244, 338, 2782
Cfr10I	RCCGGY	2 cut(s)	150, 215
Cfr13I	GGNCC	5 cut(s)	486, 583, 893, 1979, 2953
CseI	GACGC	3 cut(s)	156, 1943, 2599
Csp6I	GTAC	4 cut(s)	238, 1511, 2050, 2818
CspAI	ACCGGT	1 cut(s)	215
CviAII	CATG	8 cut(s)	53, 557, 1583, 1851, 2032, 2096, 2117, 2878
CviQI	GTAC	4 cut(s)	238, 1511, 2050, 2818
DpnI	GATC	8 cut(s)	260, 389, 1059, 1927, 2172, 2235, 2535, 2832
DpnII	GATC	8 cut(s)	258, 387, 1057, 1925, 2170, 2233, 2533, 2830
DriI	GACNNNNNGTC	1 cut(s)	1549
EaeI	YGGCCR	1 cut(s)	753
Eam1104I	CTCTTC	2 cut(s)	43, 1023
Eam1105I	GACNNNNNGTC	1 cut(s)	1549
EarI	CTCTTC	2 cut(s)	43, 1023
EciI	GGCGGA	1 cut(s)	1800
Ecl136II	GAGCTC	1 cut(s)	304
Eco130I	CCWWGG	3 cut(s)	354, 625, 1705
Eco147I	AGGCCT	1 cut(s)	417
Eco24I	GRGCYC	2 cut(s)	306, 2428
Eco31I	GGTCTC	1 cut(s)	2391
Eco32I	GATATC	1 cut(s)	2488
Eco47I	GGWCC	4 cut(s)	486, 893, 1979, 2953
Eco47III	AGCGCT	1 cut(s)	2781
Eco53kI	GAGCTC	1 cut(s)	304
Eco57I	CTGAAG	4 cut(s)	927, 1047, 1800, 3027
Eco91I	GGTNACC	1 cut(s)	3008
EcoICRI	GAGCTC	1 cut(s)	304
EcoO109I	RGGNCCY	1 cut(s)	1979
EcoO65I	GGTNACC	1 cut(s)	3008
EcoRII	CCWGG	4 cut(s)	227, 1823, 2173, 2697
EcoRV	GATATC	1 cut(s)	2488
EcoT14I	CCWWGG	3 cut(s)	354, 625, 1705
EcoT22I	ATGCAT	1 cut(s)	538
EcoT38I	GRGCYC	2 cut(s)	306, 2428
ErhI	CCWWGG	3 cut(s)	354, 625, 1705
Esp3I	CGTCTC	1 cut(s)	139
FaeI	CATG	8 cut(s)	56, 560, 1586, 1854, 2035, 2099, 2120, 2881
FalI	AAGNNNNNCTT	6 cut(s)	754, 786, 1140, 1172, 2756, 2788
FaqI	GGGAC	6 cut(s)	220, 408, 1376, 1992, 2119, 2966
FatI	CATG	8 cut(s)	52, 556, 1582, 1850, 2031, 2095, 2116, 2877
FauI	CCCGC	5 cut(s)	56, 111, 185, 1070, 1455
FauNDI	CATATG	1 cut(s)	706
FbaI	TGATCA	2 cut(s)	258, 387
Fnu4HI	GCNGC	8 cut(s)	233, 448, 930, 1338, 1446, 1742, 3048, 3051
FokI	GGATG	8 cut(s)	839, 989, 1433, 2003, 2495, 2609, 2732, 2960
FriOI	GRGCYC	2 cut(s)	306, 2428
Fsp4HI	GCNGC	8 cut(s)	233, 448, 930, 1338, 1446, 1742, 3048, 3051
FspBI	CTAG	7 cut(s)	909, 1037, 1485, 1527, 1652, 2309, 3066
GlaI	GCGC	3 cut(s)	243, 337, 2781
GluI	GCNGC	8 cut(s)	233, 448, 930, 1338, 1446, 1742, 3048, 3051
GsaI	CCCAGC	2 cut(s)	191, 2533
GsuI	CTGGAG	1 cut(s)	1286
HaeII	RGCGCY	1 cut(s)	2783
HaeIII	GGCC	4 cut(s)	417, 584, 642, 755
HapII	CCGG	7 cut(s)	99, 142, 151, 216, 489, 2594, 2705
HgaI	GACGC	3 cut(s)	156, 1943, 2599
HhaI	GCGC	3 cut(s)	244, 338, 2782
Hin1I	GRCGYC	3 cut(s)	148, 432, 2591
Hin1II	CATG	8 cut(s)	56, 560, 1586, 1854, 2035, 2099, 2120, 2881
Hin6I	GCGC	3 cut(s)	242, 336, 2780
HinP1I	GCGC	3 cut(s)	242, 336, 2780
HindIII	AAGCTT	3 cut(s)	287, 1373, 2849
HpaII	CCGG	7 cut(s)	99, 142, 151, 216, 489, 2594, 2705
HphI	GGTGA	5 cut(s)	21, 22, 230, 556, 1208
Hpy166II	GTNNAC	5 cut(s)	225, 671, 1173, 2052, 2183
Hpy8I	GTNNAC	5 cut(s)	225, 671, 1173, 2052, 2183
Hpy99I	CGWCG	1 cut(s)	591
HpyCH4IV	ACGT	4 cut(s)	432, 589, 652, 2146
HpyF10VI	GCNNNNNNNGC	9 cut(s)	147, 241, 938, 1100, 1195, 1229, 1750, 1889, 2599
HpySE526I	ACGT	4 cut(s)	432, 589, 652, 2146
Hsp92I	GRCGYC	3 cut(s)	148, 432, 2591
Hsp92II	CATG	8 cut(s)	56, 560, 1586, 1854, 2035, 2099, 2120, 2881
HspAI	GCGC	3 cut(s)	242, 336, 2780
KroI	GCCGGC	1 cut(s)	150
KroNI	GCCGGC	1 cut(s)	152
Ksp22I	TGATCA	2 cut(s)	258, 387
Kzo9I	GATC	8 cut(s)	258, 387, 1057, 1925, 2170, 2233, 2533, 2830
LmnI	GCTCC	5 cut(s)	802, 1936, 1951, 2607, 2767
Lsp1109I	GCAGC	7 cut(s)	244, 916, 1349, 1457, 1728, 3037, 3059
LweI	GCATC	6 cut(s)	1012, 1085, 1283, 1740, 2283, 2710
MaeI	CTAG	7 cut(s)	909, 1037, 1485, 1527, 1652, 2309, 3066
MaeII	ACGT	4 cut(s)	432, 589, 652, 2146
MalI	GATC	8 cut(s)	260, 389, 1059, 1927, 2172, 2235, 2535, 2832
MboI	GATC	8 cut(s)	258, 387, 1057, 1925, 2170, 2233, 2533, 2830
MfeI	CAATTG	2 cut(s)	1386, 2280
MflI	RGATCY	3 cut(s)	2170, 2533, 2830
MhlI	GDGCHC	3 cut(s)	306, 2428, 2772
MlsI	TGGCCA	1 cut(s)	755
MluNI	TGGCCA	1 cut(s)	755
MmeI	TCCRAC	5 cut(s)	66, 136, 318, 460, 673
Mox20I	TGGCCA	1 cut(s)	755
Mph1103I	ATGCAT	1 cut(s)	538
MreI	CGCCGGCG	1 cut(s)	150
MroNI	GCCGGC	1 cut(s)	150
MroXI	GAANNNNTTC	2 cut(s)	1032, 1113
MscI	TGGCCA	1 cut(s)	755
MseI	TTAA	5 cut(s)	539, 1377, 1833, 2195, 2249
Msp20I	TGGCCA	1 cut(s)	755
MspA1I	CMGCKG	2 cut(s)	2323, 3050
MspCI	CTTAAG	1 cut(s)	2194
MspI	CCGG	7 cut(s)	99, 142, 151, 216, 489, 2594, 2705
MspR9I	CCNGG	5 cut(s)	229, 1825, 2175, 2699, 2706
MunI	CAATTG	2 cut(s)	1386, 2280
Mva1269I	GAATGC	5 cut(s)	538, 1096, 1494, 1575, 1720
MvaI	CCWGG	4 cut(s)	229, 1825, 2175, 2699
MvnI	CGCG	3 cut(s)	242, 336, 1065
MwoI	GCNNNNNNNGC	9 cut(s)	147, 241, 938, 1100, 1195, 1229, 1750, 1889, 2599
NaeI	GCCGGC	1 cut(s)	152
NciI	CCSGG	1 cut(s)	2706
NdeI	CATATG	1 cut(s)	706
NdeII	GATC	8 cut(s)	258, 387, 1057, 1925, 2170, 2233, 2533, 2830
NgoMIV	GCCGGC	1 cut(s)	150
NlaIII	CATG	8 cut(s)	56, 560, 1586, 1854, 2035, 2099, 2120, 2881
NlaIV	GGNNCC	5 cut(s)	1980, 2172, 2703, 2832, 2954
NmeAIII	GCCGAG	1 cut(s)	2153
NmuCI	GTSAC	5 cut(s)	562, 695, 709, 1541, 2986
NsiI	ATGCAT	1 cut(s)	538
NspV	TTCGAA	1 cut(s)	2353
PagI	TCATGA	1 cut(s)	2031
PceI	AGGCCT	1 cut(s)	417
PctI	GAATGC	5 cut(s)	538, 1096, 1494, 1575, 1720
PdiI	GCCGGC	1 cut(s)	152
PdmI	GAANNNNTTC	2 cut(s)	1032, 1113
PfeI	GAWTC	5 cut(s)	848, 1033, 1249, 2120, 2432
PflFI	GACNNNGTC	1 cut(s)	250
PfoI	TCCNGGA	2 cut(s)	1823, 2173
PinAI	ACCGGT	1 cut(s)	215
PkrI	GCNGC	8 cut(s)	234, 449, 931, 1339, 1447, 1743, 3049, 3052
Ppu21I	YACGTR	1 cut(s)	2147
PpuMI	RGGWCCY	1 cut(s)	1979
PsiI	TTATAA	2 cut(s)	924, 2456
Psp124BI	GAGCTC	1 cut(s)	306
Psp5II	RGGWCCY	1 cut(s)	1979
Psp6I	CCWGG	4 cut(s)	227, 1823, 2173, 2697
PspEI	GGTNACC	1 cut(s)	3008
PspFI	CCCAGC	2 cut(s)	187, 2529
PspGI	CCWGG	4 cut(s)	227, 1823, 2173, 2697
PspN4I	GGNNCC	5 cut(s)	1980, 2172, 2703, 2832, 2954
PspPI	GGNCC	5 cut(s)	486, 583, 893, 1979, 2953
PspPPI	RGGWCCY	1 cut(s)	1979
PstI	CTGCAG	3 cut(s)	324, 901, 1339
PstNI	CAGNNNCTG	2 cut(s)	1343, 3050
PsuI	RGATCY	3 cut(s)	2170, 2533, 2830
PsyI	GACNNNGTC	1 cut(s)	250
PvuII	CAGCTG	1 cut(s)	3050
RsaI	GTAC	4 cut(s)	239, 1512, 2051, 2819
RsaNI	GTAC	4 cut(s)	238, 1511, 2050, 2818
SacI	GAGCTC	1 cut(s)	306
SaqAI	TTAA	5 cut(s)	539, 1377, 1833, 2195, 2249
SatI	GCNGC	8 cut(s)	233, 448, 930, 1338, 1446, 1742, 3048, 3051
Sau3AI	GATC	8 cut(s)	258, 387, 1057, 1925, 2170, 2233, 2533, 2830
Sau96I	GGNCC	5 cut(s)	486, 583, 893, 1979, 2953
ScrFI	CCNGG	5 cut(s)	229, 1825, 2175, 2699, 2706
SduI	GDGCHC	3 cut(s)	306, 2428, 2772
SfaNI	GCATC	6 cut(s)	1012, 1085, 1283, 1740, 2283, 2710
SfcI	CTRYAG	4 cut(s)	320, 897, 1335, 1449
SfuI	TTCGAA	1 cut(s)	2353
SgrAI	CRCCGGYG	1 cut(s)	150
SinI	GGWCC	4 cut(s)	486, 893, 1979, 2953
SmlI	CTYRAG	3 cut(s)	811, 2194, 2783
SmoI	CTYRAG	3 cut(s)	811, 2194, 2783
SpeI	ACTAGT	1 cut(s)	1526
SseBI	AGGCCT	1 cut(s)	417
SspI	AATATT	3 cut(s)	2503, 2567, 2749
SspMI	CTAG	7 cut(s)	909, 1037, 1485, 1527, 1652, 2309, 3066
SstI	GAGCTC	1 cut(s)	306
StuI	AGGCCT	1 cut(s)	417
StyD4I	CCNGG	5 cut(s)	227, 1823, 2173, 2697, 2704
StyI	CCWWGG	3 cut(s)	354, 625, 1705
TaiI	ACGT	4 cut(s)	435, 592, 655, 2149
TaqI	TCGA	8 cut(s)	137, 401, 1687, 2152, 2268, 2346, 2353, 2653
TaqII	GACCGA	1 cut(s)	2769
TatI	WGTACW	1 cut(s)	1510
TauI	GCSGC	1 cut(s)	450
TfiI	GAWTC	5 cut(s)	848, 1033, 1249, 2120, 2432
Tru1I	TTAA	5 cut(s)	539, 1377, 1833, 2195, 2249
Tru9I	TTAA	5 cut(s)	539, 1377, 1833, 2195, 2249
TscAI	CASTG	4 cut(s)	171, 951, 1076, 1803
TseFI	GTSAC	5 cut(s)	562, 695, 709, 1541, 2986
TseI	GCWGC	7 cut(s)	232, 929, 1337, 1445, 1741, 3047, 3050
Tsp45I	GTSAC	5 cut(s)	562, 695, 709, 1541, 2986
TspGWI	ACGGA	4 cut(s)	145, 773, 1949, 2636
TspRI	CASTG	4 cut(s)	171, 951, 1076, 1803
Tth111I	GACNNNGTC	1 cut(s)	250
Vha464I	CTTAAG	1 cut(s)	2194
VpaK11BI	GGWCC	4 cut(s)	486, 893, 1979, 2953
XapI	RAATTY	5 cut(s)	817, 1355, 1721, 1897, 2369
XbaI	TCTAGA	1 cut(s)	1484
XmnI	GAANNNNTTC	2 cut(s)	1032, 1113
XspI	CTAG	7 cut(s)	909, 1037, 1485, 1527, 1652, 2309, 3066
ZraI	GACGTC	1 cut(s)	433
Zsp2I	ATGCAT	1 cut(s)	538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.