Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0005400

Physical Location & Seq

Genomic Coordinates Forward (+)

18525753 .. 18532314

Gene Structure 6562 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023125

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3093 bp

ATGGCTCTGGTGAAGCCCCATTCCCTCTCACCTCTCTCTTCCAACTCCCGCCATGCCCTCCCCCTCAACAACAACTCCCACTTCCGCACTTCTTTCTTCGCCGGAAAACCCTTCCGACTGCCGGAAACCTCACGGATTTCGAGCCGGAGACGCCGGCGAGTGCAGGCAGTGGCTTCGCTGGGAGGTTTGTTGGGCGGGATTTTCAAAGGGACTGACACCGGTGAGTCCACCAGGCAGCAGTACGCTCAGACTGTGTCTCTGATCAACGGCTTGGAAGCTCAGATGTCTAAGCTTTCCGATTCCGAGCTCAGGGAGAAGACTCTGCAGTTTCAACAACGTGCCAAGCAGGGCGAGTCCTTGGACTCTCTTTTACCTGAAGCATTTGCTGTGATCAGAGAGGCTTCGAGGAGGGTTTTAGGCCTTCGTCCCTTTGACGTCCAACTCATAGGCGGCATAGTTCTTCACAAGGGGGAAATAGCTGAAATGAGGACTGGAGAAGGAAAGACTCTTGTTGCTATATTACCAGCGTATTTGAATGCATTAACTGGAAAGGGAGTTCATGTTGTCACTGTCAATGATTATCTGGCCCGACGTGATTGTGAGTGGGTTGGTCAAGTTCCTCGTTTCCTTGGATTGAAGGTTGGCCTAATCCAACAGAATATGACAAGTGAACAAAGAAGGGAGAACTACTTATGTGACATCACATATGTCACAAACAGCGAGCTTGGTTTTGATTTTCTGAGAGATAATCTGGCCACGGAAAAGTTTTCTTACCTAAAATTATTCTACCTCAATTCTTGGCATATACAGAGTGTTGAGGAGCTTGTCTTGAGGAATTTCAATTACTGTGTAATTGATGAGGTTGATTCCATCCTTATTGATGAAGCAAGAACACCTCTTATCATATCTGGACCTGCAGAAAAACCCAGCGATAGGTATTATAAAGCTGCAAAGATGGCTTCAGTGTTTGAGCGAGATATACATTATACTGTGGATGAAAAGCAGAAAACAGTTCTGCTTTCAGAACAAGGATATGAAGATGCTGAAGAGATTCTAGGTGTCAAAGATTTATATGATCCCCGCGAACAGTGGGCATCTTATGTTCTGAATGCCGTGAAAGCAAAAGAACTGTTTCTTAGAGATGTAAATTATATAATTCGTGGCAAAGAGGTTCTTATTGTTGATGAGTTTACAGGACGAGTTATGCAGGGGAGGCGGTGGAGTGATGGACTTCACCAAGCAGTTGAAGCAAAAGAAGGTCTGCCGATTCAAAATGAAACTGTAACTCTGGCATCTATTAGCTACCAAAACTTCTTTCTCCAGTTTCCAAAACTTTGTGGAATGACTGGAACTGCAGCAACTGAAAGCACAGAATTTGAAAGCATATACAAGCTTAAAGTTACAATTGTCCCCACAAACAAGCCTATGATAAGAAAGGATGAGTCTGATGTAGTTTTCAGGGCAGCTACAGGTAAATGGCGGGCAGTTGTAGTAGAGATTTCTAGAATGCACAAAACAGGTCGCCCTGTACTTGTTGGCACAACTAGTGTTGAGCAGAGTGACTCATTGTCTGAGCAGTTGCAAGAAGTTGGCATTCCCCATGAGGTTCTCAACGCAAAACCAGAGAATGTGGAGAGGGAAGCAGAAATTGTGGCACAAAGCGGTCGTCTAGGGGCAGTTACTATTGCAACCAATATGGCAGGTCGAGGAACAGACATAATCCTTGGTGGGAATGCAGAATTTATGGCGAGGTTGAAGCTGCGTGAGATGCTTATGCCAAGAGTTGTAAAACTAACTGAAGGAGGTTATGTCTCAGTGAAAAAACTTCCGCTGAAAAAGTCCTGGAAGGTTAATGAAAAGCTATTTCCATGCAAACTGTCCAATGAAAAGACCAAGTTAGCTGAGGAAGCTGTGAATTTAGCTGTTGAAACTTGGGGTCAGAGATCATTGACGGAGCTTGAAGCAGAGGAGCGTCTTTCTTATTCTTGCGAAAAGGGACCTGCTTTGGATGAAGTGATAGCCAAACTGCGGAGTGCTTTTTTAGAAATCATGAAAGAATATAAGGGCTACACAGAGGAAGAAAGGAAGAAGGTTGTGTCAGCTGGTGGGCTTCATGTTGTCGGGACAGAACGCCATGAATCTCGCCGAATTGATAATCAGTTACGTGGTCGAACTGGACGGCAGGGGGATCCTGGAAGTTCGCGCTTCTTCTTGAGTCTTGAAGATAACATCTTTCGTATATTTGGTGGAGATCGTATTCAGGGTTTAATGAGAGCTTTTAGAGTTGAGGACTTGCCAATTGAGTCCAAGATGCTGACCAAAGCACTAGATGAAGCTCAGCGGAAAGTGGAAAACTATTTTTTCGATATTCGAAAGCAATTGTTTGAATTTGATGAAGTCCTAAACAGCCAAAGAGACCGTGTGTATACAGAAAGAAGGAGAGCCCTTGAATCTGACAATCTGCAATCACTTATAATTGAATATGCTGAACTAACAATGGATGATATCTTAGAGGCAAATATTGGTTCAGAGTCTTCAAAAGAAAGCTGGGATCTTGATAAGCTCATCAAAAAACTTCAACAATATTGCTATCTATTGAATGATTTGACGCCCGATGTGCTGAGGAGCGAATGTTCAAGTTATGAAGATTTGCAGGACTACCTCCGTCTTCGAGGCCGTGAAGCATATTTGCAGAAAAGGACCATTATCGAGAGCCAGGCACCTGGCTTGATGAAGGATGCTGAACGGTTCTTAGTTTTGAACAATATTGACCGATTGTGGAAGGAGCACTTACAAGCACTCAAGTTCGTTCAGCAAGCTGTTGGTTTGCGTGGGTATGCACAACGGGATCCACTTATTGAATATAAGCTTGAAGGCTACAATCTTTTCTTGGACATGATGGCACAGATAAGAAGAAATGTTATATATTCCATATACCAGTTTCAACCTGTGATGGTAAAGAAAGATGGGGACCAAAGAGAGAATAAGAAATCAGGAAAACTTGTCACTAATGGTAGCGGTAATGGTAACCCCAATTCAGTTGGCAGTGTTGAGTCTTCTTCAGCAGCTGCCACCCCCCAATCTAGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071806 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

1031

Amino Acids

116.75

Weight (kDa)

7.56

Isoelectric Point (pI)

47.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	75 - 460	4.2e-122	SecA DEAD-like domain
DEAD	PF00270	159 - 293	9.9e-06	DEAD/DEAH box helicase
SecA_PP_bind	PF01043	310 - 416	1.1e-37	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	475 - 764	7.9e-83	SecA P-loop domain
SecA_SW	PF07516	766 - 982	2.9e-56	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01800 AT4G01800 AT4G01800
fragaria_vesca	FvH4_1g26240
malus_domestica	MD17G1285000.v1.1
prunus_persica	Prupe.3G002700_v2.0.a1
pyrus_communis	pycom09g19850 pycom17g28080 pycom17g28090
rosa_chinensis	RchiOBHm_Chr3g0485781
rosa_laevigata	RLG00000023125
rosa_multiflora	Rmu_co8471925.1_g000001 Rmu_sc0012870.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123840
rosa_rugosa	Rorug03G0221400
rosa_samantha	Rh1AG180100 Rh1CG167100 Rh3AG270700 Rh3BG306200 Rh3BG306300 Rh3CG304600 Rh3DG300700 Rh4AG241300 Rh4AG241400 Rh4BG244600 Rh4BG244700 Rh4CG255400 Rh7CG119700
rosa_wichuraiana	Rw3G024110

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	942, 2474
AatII	GACGTC	1 cut(s)	438
Acc36I	ACCTGC	3 cut(s)	922, 1691, 2008
AccB1I	GGYRCC	1 cut(s)	2719
AccI	GTMKAC	1 cut(s)	2426
AccII	CGCG	2 cut(s)	1083, 2203
AclWI	GGATC	6 cut(s)	1070, 2183, 2196, 2559, 2843, 2856
AcoI	YGGCCR	1 cut(s)	753
AcsI	RAATTY	5 cut(s)	835, 1373, 1739, 1915, 2387
AcuI	CTGAAG	5 cut(s)	396, 945, 1065, 1818, 3045
AcyI	GRCGYC	3 cut(s)	151, 435, 2609
AfaI	GTAC	2 cut(s)	242, 1530
AfiI	CCNNNNNNNGG	4 cut(s)	121, 309, 933, 2028
AgeI	ACCGGT	1 cut(s)	218
AhdI	GACNNNNNGTC	1 cut(s)	1567
AhlI	ACTAGT	1 cut(s)	1544
AjiI	CACGTC	1 cut(s)	593
AjnI	CCWGG	5 cut(s)	230, 1841, 2191, 2715, 2722
AjuI	GAANNNNNNNTTGG	2 cut(s)	1299, 1331
AloI	GAACNNNNNNTCC	4 cut(s)	540, 572, 2617, 2649
Alw21I	GWGCWC	2 cut(s)	309, 2790
Alw26I	GTCTC	4 cut(s)	142, 261, 1816, 2409
AlwI	GGATC	6 cut(s)	1070, 2183, 2196, 2559, 2843, 2856
AlwNI	CAGNNNCTG	2 cut(s)	1361, 3068
AoxI	GGCC	5 cut(s)	418, 585, 643, 753, 2674
ApeKI	GCWGC	7 cut(s)	235, 947, 1355, 1463, 1759, 3065, 3068
ApoI	RAATTY	5 cut(s)	835, 1373, 1739, 1915, 2387
ArsI	GACNNNNNNTTYG	2 cut(s)	1651, 1683
AsiGI	ACCGGT	1 cut(s)	218
Asp700I	GAANNNNTTC	2 cut(s)	1050, 1131
AspLEI	GCGC	1 cut(s)	2205
AspS9I	GGNCC	5 cut(s)	586, 911, 1997, 2700, 2971
AsuHPI	GGTGA	4 cut(s)	21, 22, 233, 1226
AsuII	TTCGAA	1 cut(s)	2371
AvaII	GGWCC	4 cut(s)	911, 1997, 2700, 2971
BalI	TGGCCA	1 cut(s)	755
BamHI	GGATCC	2 cut(s)	2188, 2848
BanI	GGYRCC	1 cut(s)	2719
BanII	GRGCYC	2 cut(s)	309, 2446
BbsI	GAAGAC	4 cut(s)	323, 2526, 2660, 3048
Bbv12I	GWGCWC	2 cut(s)	309, 2790
BbvCI	CCTCAGC	2 cut(s)	1902, 2621
BbvI	GCAGC	7 cut(s)	247, 934, 1367, 1475, 1746, 3055, 3077
BccI	CCATC	6 cut(s)	878, 949, 1220, 2893, 2947, 2960
BceAI	ACGGC	4 cut(s)	283, 1097, 2195, 2661
BcgI	CGANNNNNNTGC	2 cut(s)	156, 190
BciT130I	CCWGG	5 cut(s)	232, 1843, 2193, 2717, 2724
BclI	TGATCA	2 cut(s)	261, 390
BcoDI	GTCTC	4 cut(s)	142, 261, 1816, 2409
BcuI	ACTAGT	1 cut(s)	1544
BfaI	CTAG	6 cut(s)	1055, 1503, 1545, 1670, 2327, 3084
BfmI	CTRYAG	4 cut(s)	323, 915, 1353, 1467
BfuAI	ACCTGC	3 cut(s)	922, 1691, 2008
BisI	GCNGC	8 cut(s)	236, 451, 948, 1356, 1464, 1760, 3066, 3069
BlpI	GCTNAGC	1 cut(s)	2337
BlsI	GCNGC	8 cut(s)	237, 452, 949, 1357, 1465, 1761, 3067, 3070
Bme1390I	CCNGG	5 cut(s)	232, 1843, 2193, 2717, 2724
Bme18I	GGWCC	4 cut(s)	911, 1997, 2700, 2971
BmeRI	GACNNNNNGTC	1 cut(s)	1567
BmgBI	CACGTC	1 cut(s)	593
BmgT120I	GGNCC	5 cut(s)	586, 911, 1997, 2700, 2971
BmiI	GGNNCC	5 cut(s)	1998, 2190, 2721, 2850, 2972
BmrFI	CCNGG	5 cut(s)	232, 1843, 2193, 2717, 2724
BmsI	GCATC	6 cut(s)	1030, 1103, 1301, 1758, 2301, 2728
BpiI	GAAGAC	4 cut(s)	323, 2526, 2660, 3048
BpmI	CTGGAG	2 cut(s)	513, 1304
Bpu10I	CCTNAGC	3 cut(s)	308, 1902, 2621
Bpu1102I	GCTNAGC	1 cut(s)	2337
Bpu14I	TTCGAA	1 cut(s)	2371
BpuEI	CTTGAG	3 cut(s)	850, 2233, 2786
BsaAI	YACGTR	1 cut(s)	2165
BsaHI	GRCGYC	3 cut(s)	151, 435, 2609
BsaI	GGTCTC	1 cut(s)	2409
BsaJI	CCNNGG	4 cut(s)	357, 628, 756, 1723
BsaWI	WCCGGW	1 cut(s)	218
BsaXI	ACNNNNNCTCC	8 cut(s)	59, 89, 546, 576, 1794, 1824, 2617, 2647
Bsc4I	CCNNNNNNNGG	4 cut(s)	121, 309, 933, 2028
Bse118I	RCCGGY	2 cut(s)	153, 218
Bse1I	ACTGG	6 cut(s)	496, 550, 1321, 1351, 2179, 2938
BseBI	CCWGG	5 cut(s)	232, 1843, 2193, 2717, 2724
BseDI	CCNNGG	4 cut(s)	357, 628, 756, 1723
BseGI	GGATG	6 cut(s)	870, 1000, 1444, 2014, 2506, 2743
BseLI	CCNNNNNNNGG	4 cut(s)	121, 309, 933, 2028
BseMII	CTCAG	9 cut(s)	260, 293, 322, 731, 1563, 1827, 1893, 2351, 2612
BseNI	ACTGG	6 cut(s)	496, 550, 1321, 1351, 2179, 2938
BseRI	GAGGAG	4 cut(s)	421, 833, 1982, 2638
BseXI	GCAGC	7 cut(s)	247, 934, 1367, 1475, 1746, 3055, 3077
BseYI	CCCAGC	3 cut(s)	178, 926, 2547
BsgI	GTGCAG	1 cut(s)	182
Bsh1236I	CGCG	2 cut(s)	1083, 2203
Bsh1285I	CGRYCG	1 cut(s)	1666
BshFI	GGCC	5 cut(s)	420, 587, 645, 755, 2676
BshNI	GGYRCC	1 cut(s)	2719
BshTI	ACCGGT	1 cut(s)	218
BsiEI	CGRYCG	1 cut(s)	1666
BsiHKAI	GWGCWC	2 cut(s)	309, 2790
BsiSI	CCGG	5 cut(s)	102, 122, 145, 154, 219
BslFI	GGGAC	6 cut(s)	223, 411, 1394, 2010, 2137, 2984
BslI	CCNNNNNNNGG	4 cut(s)	121, 309, 933, 2028
BsmAI	GTCTC	4 cut(s)	142, 261, 1816, 2409
BsmBI	CGTCTC	1 cut(s)	142
BsmFI	GGGAC	6 cut(s)	223, 411, 1394, 2010, 2137, 2984
BsmI	GAATGC	5 cut(s)	541, 1114, 1512, 1593, 1738
BsnI	GGCC	5 cut(s)	420, 587, 645, 755, 2676
Bso31I	GGTCTC	1 cut(s)	2409
Bsp119I	TTCGAA	1 cut(s)	2371
Bsp1286I	GDGCHC	3 cut(s)	309, 2446, 2790
Bsp143I	GATC	8 cut(s)	261, 390, 1075, 1943, 2188, 2251, 2551, 2848
Bsp1720I	GCTNAGC	1 cut(s)	2337
BspANI	GGCC	5 cut(s)	420, 587, 645, 755, 2676
BspCNI	CTCAG	9 cut(s)	259, 292, 321, 732, 1564, 1826, 1894, 2350, 2613
BspFNI	CGCG	2 cut(s)	1083, 2203
BspHI	TCATGA	1 cut(s)	2049
BspLI	GGNNCC	5 cut(s)	1998, 2190, 2721, 2850, 2972
BspMAI	CTGCAG	3 cut(s)	327, 919, 1357
BspMI	ACCTGC	3 cut(s)	922, 1691, 2008
BspPI	GGATC	6 cut(s)	1070, 2183, 2196, 2559, 2843, 2856
BspT104I	TTCGAA	1 cut(s)	2371
BspT107I	GGYRCC	1 cut(s)	2719
BspTNI	GGTCTC	1 cut(s)	2409
BsrFI	RCCGGY	2 cut(s)	153, 218
BsrI	ACTGG	6 cut(s)	496, 550, 1321, 1351, 2179, 2938
BssAI	RCCGGY	2 cut(s)	153, 218
BssECI	CCNNGG	4 cut(s)	357, 628, 756, 1723
BssMI	GATC	8 cut(s)	261, 390, 1075, 1943, 2188, 2251, 2551, 2848
BssNAI	GTATAC	1 cut(s)	2427
BssNI	GRCGYC	3 cut(s)	151, 435, 2609
BssT1I	CCWWGG	3 cut(s)	357, 628, 1723
Bst1107I	GTATAC	1 cut(s)	2427
Bst2UI	CCWGG	5 cut(s)	232, 1843, 2193, 2717, 2724
Bst6I	CTCTTC	2 cut(s)	43, 1041
BstACI	GRCGYC	3 cut(s)	151, 435, 2609
BstBAI	YACGTR	1 cut(s)	2165
BstBI	TTCGAA	1 cut(s)	2371
BstC8I	GCNNGC	5 cut(s)	155, 165, 722, 1482, 2817
BstDSI	CCRYGG	1 cut(s)	756
BstEII	GGTNACC	1 cut(s)	3026
BstF5I	GGATG	6 cut(s)	870, 1000, 1444, 2014, 2506, 2743
BstFNI	CGCG	2 cut(s)	1083, 2203
BstHHI	GCGC	1 cut(s)	2205
BstKTI	GATC	8 cut(s)	264, 393, 1078, 1946, 2191, 2254, 2554, 2851
BstMAI	GTCTC	4 cut(s)	142, 261, 1816, 2409
BstMBI	GATC	8 cut(s)	261, 390, 1075, 1943, 2188, 2251, 2551, 2848
BstMCI	CGRYCG	1 cut(s)	1666
BstMWI	GCNNNNNNNGC	8 cut(s)	150, 956, 1118, 1213, 1247, 1768, 1907, 2617
BstNI	CCWGG	5 cut(s)	232, 1843, 2193, 2717, 2724
BstPI	GGTNACC	1 cut(s)	3026
BstSCI	CCNGG	5 cut(s)	230, 1841, 2191, 2715, 2722
BstSFI	CTRYAG	4 cut(s)	323, 915, 1353, 1467
BstUI	CGCG	2 cut(s)	1083, 2203
BstV1I	GCAGC	7 cut(s)	247, 934, 1367, 1475, 1746, 3055, 3077
BstV2I	GAAGAC	4 cut(s)	323, 2526, 2660, 3048
BstX2I	RGATCY	3 cut(s)	2188, 2551, 2848
BstXI	CCANNNNNNTGG	1 cut(s)	2723
BstYI	RGATCY	3 cut(s)	2188, 2551, 2848
BstZ17I	GTATAC	1 cut(s)	2427
BsuRI	GGCC	5 cut(s)	420, 587, 645, 755, 2676
BtgI	CCRYGG	1 cut(s)	756
BtrI	CACGTC	1 cut(s)	593
BtsCI	GGATG	6 cut(s)	870, 1000, 1444, 2014, 2506, 2743
BtsI	GCAGTG	2 cut(s)	174, 3052
BtsIMutI	CAGTG	6 cut(s)	174, 567, 969, 1094, 1821, 3052
BveI	ACCTGC	3 cut(s)	922, 1691, 2008
Cac8I	GCNNGC	5 cut(s)	155, 165, 722, 1482, 2817
CaiI	CAGNNNCTG	2 cut(s)	1361, 3068
CciI	TCATGA	1 cut(s)	2049
CfoI	GCGC	1 cut(s)	2205
Cfr10I	RCCGGY	2 cut(s)	153, 218
Cfr13I	GGNCC	5 cut(s)	586, 911, 1997, 2700, 2971
CseI	GACGC	3 cut(s)	159, 1961, 2617
Csp6I	GTAC	2 cut(s)	241, 1529
CspAI	ACCGGT	1 cut(s)	218
CviAII	CATG	8 cut(s)	53, 560, 1601, 1869, 2050, 2114, 2135, 2896
CviQI	GTAC	2 cut(s)	241, 1529
DpnI	GATC	8 cut(s)	263, 392, 1077, 1945, 2190, 2253, 2553, 2850
DpnII	GATC	8 cut(s)	261, 390, 1075, 1943, 2188, 2251, 2551, 2848
DriI	GACNNNNNGTC	1 cut(s)	1567
EaeI	YGGCCR	1 cut(s)	753
Eam1104I	CTCTTC	2 cut(s)	43, 1041
Eam1105I	GACNNNNNGTC	1 cut(s)	1567
EarI	CTCTTC	2 cut(s)	43, 1041
Ecl136II	GAGCTC	1 cut(s)	307
Eco130I	CCWWGG	3 cut(s)	357, 628, 1723
Eco147I	AGGCCT	1 cut(s)	420
Eco24I	GRGCYC	2 cut(s)	309, 2446
Eco31I	GGTCTC	1 cut(s)	2409
Eco32I	GATATC	1 cut(s)	2506
Eco47I	GGWCC	4 cut(s)	911, 1997, 2700, 2971
Eco53kI	GAGCTC	1 cut(s)	307
Eco57I	CTGAAG	5 cut(s)	396, 945, 1065, 1818, 3045
Eco91I	GGTNACC	1 cut(s)	3026
EcoICRI	GAGCTC	1 cut(s)	307
EcoO109I	RGGNCCY	1 cut(s)	1997
EcoO65I	GGTNACC	1 cut(s)	3026
EcoRII	CCWGG	5 cut(s)	230, 1841, 2191, 2715, 2722
EcoRV	GATATC	1 cut(s)	2506
EcoT14I	CCWWGG	3 cut(s)	357, 628, 1723
EcoT22I	ATGCAT	1 cut(s)	541
EcoT38I	GRGCYC	2 cut(s)	309, 2446
ErhI	CCWWGG	3 cut(s)	357, 628, 1723
Esp3I	CGTCTC	1 cut(s)	142
FaeI	CATG	8 cut(s)	56, 563, 1604, 1872, 2053, 2117, 2138, 2899
FalI	AAGNNNNNCTT	4 cut(s)	1158, 1190, 2774, 2806
FaqI	GGGAC	6 cut(s)	223, 411, 1394, 2010, 2137, 2984
FatI	CATG	8 cut(s)	52, 559, 1600, 1868, 2049, 2113, 2134, 2895
FauI	CCCGC	4 cut(s)	56, 188, 1088, 1473
FauNDI	CATATG	1 cut(s)	706
FbaI	TGATCA	2 cut(s)	261, 390
FblI	GTMKAC	1 cut(s)	2426
Fnu4HI	GCNGC	8 cut(s)	236, 451, 948, 1356, 1464, 1760, 3066, 3069
FokI	GGATG	6 cut(s)	857, 1007, 1451, 2021, 2513, 2750
FriOI	GRGCYC	2 cut(s)	309, 2446
Fsp4HI	GCNGC	8 cut(s)	236, 451, 948, 1356, 1464, 1760, 3066, 3069
FspBI	CTAG	6 cut(s)	1055, 1503, 1545, 1670, 2327, 3084
GlaI	GCGC	1 cut(s)	2204
GluI	GCNGC	8 cut(s)	236, 451, 948, 1356, 1464, 1760, 3066, 3069
GsaI	CCCAGC	3 cut(s)	182, 930, 2551
GsuI	CTGGAG	2 cut(s)	513, 1304
HaeIII	GGCC	5 cut(s)	420, 587, 645, 755, 2676
HapII	CCGG	5 cut(s)	102, 122, 145, 154, 219
HgaI	GACGC	3 cut(s)	159, 1961, 2617
HhaI	GCGC	1 cut(s)	2205
Hin1I	GRCGYC	3 cut(s)	151, 435, 2609
Hin1II	CATG	8 cut(s)	56, 563, 1604, 1872, 2053, 2117, 2138, 2899
Hin6I	GCGC	1 cut(s)	2203
HinP1I	GCGC	1 cut(s)	2203
HindIII	AAGCTT	3 cut(s)	290, 1391, 2867
HpaII	CCGG	5 cut(s)	102, 122, 145, 154, 219
HphI	GGTGA	4 cut(s)	21, 22, 233, 1226
Hpy166II	GTNNAC	4 cut(s)	228, 671, 1191, 2427
Hpy8I	GTNNAC	4 cut(s)	228, 671, 1191, 2427
Hpy99I	CGWCG	1 cut(s)	594
HpyCH4IV	ACGT	4 cut(s)	337, 435, 592, 2164
HpyF10VI	GCNNNNNNNGC	8 cut(s)	150, 956, 1118, 1213, 1247, 1768, 1907, 2617
HpySE526I	ACGT	4 cut(s)	337, 435, 592, 2164
Hsp92I	GRCGYC	3 cut(s)	151, 435, 2609
Hsp92II	CATG	8 cut(s)	56, 563, 1604, 1872, 2053, 2117, 2138, 2899
HspAI	GCGC	1 cut(s)	2203
KroI	GCCGGC	1 cut(s)	153
KroNI	GCCGGC	1 cut(s)	155
Ksp22I	TGATCA	2 cut(s)	261, 390
Kzo9I	GATC	8 cut(s)	261, 390, 1075, 1943, 2188, 2251, 2551, 2848
LmnI	GCTCC	5 cut(s)	820, 1954, 1969, 2625, 2785
Lsp1109I	GCAGC	7 cut(s)	247, 934, 1367, 1475, 1746, 3055, 3077
LweI	GCATC	6 cut(s)	1030, 1103, 1301, 1758, 2301, 2728
MaeI	CTAG	6 cut(s)	1055, 1503, 1545, 1670, 2327, 3084
MaeII	ACGT	4 cut(s)	337, 435, 592, 2164
MalI	GATC	8 cut(s)	263, 392, 1077, 1945, 2190, 2253, 2553, 2850
MboI	GATC	8 cut(s)	261, 390, 1075, 1943, 2188, 2251, 2551, 2848
MfeI	CAATTG	3 cut(s)	1404, 2298, 2378
MflI	RGATCY	3 cut(s)	2188, 2551, 2848
MhlI	GDGCHC	3 cut(s)	309, 2446, 2790
MlsI	TGGCCA	1 cut(s)	755
MluNI	TGGCCA	1 cut(s)	755
MmeI	TCCRAC	4 cut(s)	66, 139, 463, 676
Mox20I	TGGCCA	1 cut(s)	755
Mph1103I	ATGCAT	1 cut(s)	541
MreI	CGCCGGCG	1 cut(s)	153
MroNI	GCCGGC	1 cut(s)	153
MroXI	GAANNNNTTC	2 cut(s)	1050, 1131
MscI	TGGCCA	1 cut(s)	755
MseI	TTAA	4 cut(s)	542, 1395, 1851, 2267
Msp20I	TGGCCA	1 cut(s)	755
MspA1I	CMGCKG	4 cut(s)	1831, 2102, 2341, 3068
MspI	CCGG	5 cut(s)	102, 122, 145, 154, 219
MspR9I	CCNGG	5 cut(s)	232, 1843, 2193, 2717, 2724
MunI	CAATTG	3 cut(s)	1404, 2298, 2378
Mva1269I	GAATGC	5 cut(s)	541, 1114, 1512, 1593, 1738
MvaI	CCWGG	5 cut(s)	232, 1843, 2193, 2717, 2724
MvnI	CGCG	2 cut(s)	1083, 2203
MwoI	GCNNNNNNNGC	8 cut(s)	150, 956, 1118, 1213, 1247, 1768, 1907, 2617
NaeI	GCCGGC	1 cut(s)	155
NdeI	CATATG	1 cut(s)	706
NdeII	GATC	8 cut(s)	261, 390, 1075, 1943, 2188, 2251, 2551, 2848
NgoMIV	GCCGGC	1 cut(s)	153
NlaIII	CATG	8 cut(s)	56, 563, 1604, 1872, 2053, 2117, 2138, 2899
NlaIV	GGNNCC	5 cut(s)	1998, 2190, 2721, 2850, 2972
NmuCI	GTSAC	5 cut(s)	565, 695, 709, 1559, 3004
NsiI	ATGCAT	1 cut(s)	541
NspV	TTCGAA	1 cut(s)	2371
PagI	TCATGA	1 cut(s)	2049
PceI	AGGCCT	1 cut(s)	420
PctI	GAATGC	5 cut(s)	541, 1114, 1512, 1593, 1738
PdiI	GCCGGC	1 cut(s)	155
PdmI	GAANNNNTTC	2 cut(s)	1050, 1131
PfeI	GAWTC	6 cut(s)	299, 866, 1051, 1267, 2138, 2450
PflFI	GACNNNGTC	1 cut(s)	253
PfoI	TCCNGGA	2 cut(s)	1841, 2191
PinAI	ACCGGT	1 cut(s)	218
PkrI	GCNGC	8 cut(s)	237, 452, 949, 1357, 1465, 1761, 3067, 3070
Ppu21I	YACGTR	1 cut(s)	2165
PpuMI	RGGWCCY	1 cut(s)	1997
PsiI	TTATAA	2 cut(s)	942, 2474
Psp124BI	GAGCTC	1 cut(s)	309
Psp5II	RGGWCCY	1 cut(s)	1997
Psp6I	CCWGG	5 cut(s)	230, 1841, 2191, 2715, 2722
PspEI	GGTNACC	1 cut(s)	3026
PspFI	CCCAGC	3 cut(s)	178, 926, 2547
PspGI	CCWGG	5 cut(s)	230, 1841, 2191, 2715, 2722
PspN4I	GGNNCC	5 cut(s)	1998, 2190, 2721, 2850, 2972
PspPI	GGNCC	5 cut(s)	586, 911, 1997, 2700, 2971
PspPPI	RGGWCCY	1 cut(s)	1997
PstI	CTGCAG	3 cut(s)	327, 919, 1357
PstNI	CAGNNNCTG	2 cut(s)	1361, 3068
PsuI	RGATCY	3 cut(s)	2188, 2551, 2848
PsyI	GACNNNGTC	1 cut(s)	253
PvuII	CAGCTG	2 cut(s)	2102, 3068
RsaI	GTAC	2 cut(s)	242, 1530
RsaNI	GTAC	2 cut(s)	241, 1529
SacI	GAGCTC	1 cut(s)	309
SaqAI	TTAA	4 cut(s)	542, 1395, 1851, 2267
SatI	GCNGC	8 cut(s)	236, 451, 948, 1356, 1464, 1760, 3066, 3069
Sau3AI	GATC	8 cut(s)	261, 390, 1075, 1943, 2188, 2251, 2551, 2848
Sau96I	GGNCC	5 cut(s)	586, 911, 1997, 2700, 2971
ScrFI	CCNGG	5 cut(s)	232, 1843, 2193, 2717, 2724
SduI	GDGCHC	3 cut(s)	309, 2446, 2790
SfaNI	GCATC	6 cut(s)	1030, 1103, 1301, 1758, 2301, 2728
SfcI	CTRYAG	4 cut(s)	323, 915, 1353, 1467
SfuI	TTCGAA	1 cut(s)	2371
SgrAI	CRCCGGYG	2 cut(s)	153, 218
SinI	GGWCC	4 cut(s)	911, 1997, 2700, 2971
SmlI	CTYRAG	3 cut(s)	829, 2212, 2801
SmoI	CTYRAG	3 cut(s)	829, 2212, 2801
SpeI	ACTAGT	1 cut(s)	1544
SseBI	AGGCCT	1 cut(s)	420
SspI	AATATT	3 cut(s)	2521, 2585, 2767
SspMI	CTAG	6 cut(s)	1055, 1503, 1545, 1670, 2327, 3084
SstI	GAGCTC	1 cut(s)	309
StuI	AGGCCT	1 cut(s)	420
StyD4I	CCNGG	5 cut(s)	230, 1841, 2191, 2715, 2722
StyI	CCWWGG	3 cut(s)	357, 628, 1723
TaiI	ACGT	4 cut(s)	340, 438, 595, 2167
TaqI	TCGA	8 cut(s)	140, 404, 1705, 2170, 2364, 2371, 2671, 2709
TaqII	GACCGA	1 cut(s)	2787
TatI	WGTACW	1 cut(s)	1528
TauI	GCSGC	1 cut(s)	453
TfiI	GAWTC	6 cut(s)	299, 866, 1051, 1267, 2138, 2450
Tru1I	TTAA	4 cut(s)	542, 1395, 1851, 2267
Tru9I	TTAA	4 cut(s)	542, 1395, 1851, 2267
TscAI	CASTG	6 cut(s)	174, 574, 969, 1094, 1821, 3052
TseFI	GTSAC	5 cut(s)	565, 695, 709, 1559, 3004
TseI	GCWGC	7 cut(s)	235, 947, 1355, 1463, 1759, 3065, 3068
Tsp45I	GTSAC	5 cut(s)	565, 695, 709, 1559, 3004
TspGWI	ACGGA	4 cut(s)	148, 773, 1967, 2654
TspRI	CASTG	6 cut(s)	174, 574, 969, 1094, 1821, 3052
Tth111I	GACNNNGTC	1 cut(s)	253
VpaK11BI	GGWCC	4 cut(s)	911, 1997, 2700, 2971
XapI	RAATTY	5 cut(s)	835, 1373, 1739, 1915, 2387
XbaI	TCTAGA	1 cut(s)	1502
XmiI	GTMKAC	1 cut(s)	2426
XmnI	GAANNNNTTC	2 cut(s)	1050, 1131
XspI	CTAG	6 cut(s)	1055, 1503, 1545, 1670, 2327, 3084
ZraI	GACGTC	1 cut(s)	436
Zsp2I	ATGCAT	1 cut(s)	541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.