Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0005400

Physical Location & Seq

Genomic Coordinates Reverse (-)

31674711 .. 31681265

Gene Structure 6555 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG300700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGGCTCTGGTGAAGCCCCATTCCCTCTCACCTCTCTCTTCCAACTCCCGCCATGCCCTCCCCCTCAACAACTCCCACTTCCGCACTTCTTTCTTCGCCGGAAAACCCTTCCGACTGCCGGAAACCTCACGGATTTCGAGCCGGAGACGCCGGCGAGTGCAGGCAGTGGCTTCCCTCGGAGGTTTGTTGGGCGGGATTTTCAAAGGGACTGACACCGGTGAGTCCACCAGGCAGCAGTACGCTCAGACTGTGTCTCTGATCAACGGCTTGGAAGCTCAGATGTCTAAGCTTTCCGATTCCGAGCTCAGGGAGAAGACTCTGCAGTTTCAACAACGCGCCAAGCAGGGCGAGTCCTTGGACTCTCTTTTACCCGAAGCATTTGCTGTGATCAGAGAGGCTTCGAGGAGGGTTTTAGGCCTTCGTCCCTTTGACGTCCAACTCATAGGCGGCATAGTTCTTCACAAGGGGGAAATAGCTGAAATGAGGACTGGAGAAGGAAAGACCCTTGTTGCTATTTTACCAGCATATTTGAATGCATTAACTGGAAAGGGAGTTCATGTTGTCACCGTCAATGATTATCTGGCCCGACGTGATTGTGAGTGGGTTGGTCAAGTTCCTCGTTTCCTTGGATTGAAGGTTGGCCTAATCCAACAAAATATGACAAGTGAACAAAGAAGGGAGAACTACTTATGTGACGTCACATATGTCACAAACAGCGAGCTTGGTTTTGATTTCCTGAGAGATAATCTGGCCACGGAAAGTAATTCTTGCTATATTTTCTTAACTAATAGTGTTGAGGAGCTTGTCTTGAGGAATTTCAATTACTGTGTAATTGATGAGGTTGATTCCATCCTTATTGATGAAGCAAGAACACCTCTCATTATATCTGGACCTGCAGAAAAACCTAGTGATAGGTATTATAAAGCTGCAAAGATGGCTTCAGTGTTTGAGCGAGATATACATTATACTGTGGATGAAAAGCAGAAAACAGTTCTGCTTTCAGAACAAGGATATGAAGATGCTGAAGAGATTCTAGGTGTCAAAGATTTATATGATCCCCGCGAACAGTGGGCATCTTATGTTCTGAATGCCGTGAAAGCTAAAGAACTGTTTCTTAGAGATGTAAATTATATAATTCGTGGCAAAGAGGTTCTTATCGTTGATGAGTTTACAGGACGAGTTATGCAGGGGAGGCGGTGGAGTGATGGACTTCACCAAGCAGTTGAAGCAAAAGAAGGTCTGCCGATTCAAAACGAAACTGTAACTCTGGCATCTATTAGCTACCAAAACTTCTTTCTCCAGTTTCCAAAACTTTGTGGAATGACTGGAACTGCAGCAACTGAAAGCACAGAATTTGAAAGCATATACAAGCTTAAAGTTACAATTGTCCCCACAAACAAGCCTATGATAAGAAAGGATGAGTCTGATGTAGTTTTCAGAGCAGCTACAGGTAAATGGCGGGCGGTTGTAGTAGAGATTTCTAGAATGCACAAAACAGGTCGCCCTGTACTTGTTGGCACAACTAGTGTTGAGCAGAGTGACTCATTGTCTGAGCAGTTGCAAGAAGTTGGTATTCCCCATGAGGTTCTCAACGCAAAACCAGAGAATGTGGAGAGGGAAGCAGAAATTGTGGCACAAAGCGGTCGTCTAGGGGCAGTTACTATTGCAACCAATATGGCAGGTCGAGGAACAGACATAATCCTTGGTGGGAATGCAGAATTTATGGCGAGGTTGAAGCTGCGTGAGATGCTTATGCCAAGAGTTGTAAAACTAACTGAAGGAGGTTATGTCTCAGTGAAAAAACTTCCGCCAAAAAAGTCCTGGAAGGTTAATGAAAAGCTATTTCCATGCAAGCTGTCCAATGAAAAGACCAAGTTAGCTGAGGAAGCTGTGAATTTAGCTGTTGAAACTTGGGGTCAGAGATCATTGACGGAGCTTGAAGCAGAGGAGCGTCTTTCTTATTCTTGCGAAAAGGGACCTGCTTTGGATGAAGTGATAGCCAAACTGCGGAGTGCTTTTTTAGAAATCATGAAAGAATATAAGGGCTACACAGAGGAAGAAAGGAAGAAGGTTGTGTCAGCTGGTGGGCTTCATGTTGTCGGGACAGAACGCCATGAATCTCGCCGAGTTGATAATCAGTTACGTGGTCGAACTGGACGGCAGGGGGATCCTGGAAGTTCACGCTTCTTCTTAAGTCTTGAAGATAACATCTTTCGTATATTTGGTGGAGATCGTATTCAGGGTTTAATGAGAGCTTTTAGAGTTGAGGACTTGCCAATTGAGTCCAAGATGCTGACCAAAGCACTAGACGAAGCTCAGCGGAAAGTGGAAAACTATTTTTTCGATATTCGAAAGCAATTGTTTGAATTTGATGAAGTCCTGAACAGCCAAAGAGACCGTGTGTATACGGAAAGAAGGAGAGCCCTTGAATCTGACAATCTGCAATCACTTATAATTGAATATGCTGAACTAACAATGGATGATATCTTAGAGGCAAATATTGGTTCAGAGTCTTCAAAAGAAAGCTGGGATCTTGATAAGCTCATCAAGAAACTTCAACAGATGGGGACCAAAGAGAGAATAAGAAATCAGGAAAACTTGTCACTAATGGTAGCGGTAATGGTAACCCCAATTCAGTTGGCAGTGTTGAGTCTTCTTCAGCAGCTGCCACCCCCCAATCTAGTGCATAAACCTGACCGGATCGTTCCAGCTGAAGTTCAACAGGAAGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071806 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

911

Amino Acids

102.82

Weight (kDa)

6.43

Isoelectric Point (pI)

45.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	74 - 453	8e-123	SecA DEAD-like domain
DEAD	PF00270	158 - 286	2.7e-06	DEAD/DEAH box helicase
SecA_PP_bind	PF01043	303 - 409	8.9e-38	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	468 - 757	1.3e-82	SecA P-loop domain
SecA_SW	PF07516	759 - 857	2.3e-27	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01800 AT4G01800 AT4G01800
fragaria_vesca	FvH4_1g26240
malus_domestica	MD17G1285000.v1.1
prunus_persica	Prupe.3G002700_v2.0.a1
pyrus_communis	pycom09g19850 pycom17g28080 pycom17g28090
rosa_chinensis	RchiOBHm_Chr3g0485781
rosa_laevigata	RLG00000023125
rosa_multiflora	Rmu_co8471925.1_g000001 Rmu_sc0012870.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123840
rosa_rugosa	Rorug03G0221400
rosa_samantha	Rh1AG180100 Rh1CG167100 Rh3AG270700 Rh3BG306200 Rh3BG306300 Rh3CG304600 Rh3DG300700 Rh4AG241300 Rh4AG241400 Rh4BG244600 Rh4BG244700 Rh4CG255400 Rh7CG119700
rosa_wichuraiana	Rw3G024110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	921, 2453
AatII	GACGTC	2 cut(s)	435, 699
Acc36I	ACCTGC	3 cut(s)	901, 1670, 1987
AccI	GTMKAC	1 cut(s)	2405
AccII	CGCG	2 cut(s)	336, 1062
AclWI	GGATC	5 cut(s)	1049, 2162, 2175, 2538, 2708
AcoI	YGGCCR	1 cut(s)	750
AcsI	RAATTY	5 cut(s)	814, 1352, 1718, 1894, 2366
AcuI	CTGAAG	5 cut(s)	924, 1044, 1797, 2642, 2733
AcyI	GRCGYC	3 cut(s)	148, 432, 696
AfaI	GTAC	2 cut(s)	239, 1509
AfiI	CCNNNNNNNGG	3 cut(s)	118, 306, 2007
AflII	CTTAAG	1 cut(s)	2191
AgeI	ACCGGT	1 cut(s)	215
AhdI	GACNNNNNGTC	1 cut(s)	1546
AhlI	ACTAGT	1 cut(s)	1523
AjiI	CACGTC	1 cut(s)	590
AjnI	CCWGG	3 cut(s)	227, 1820, 2170
AjuI	GAANNNNNNNTTGG	2 cut(s)	1278, 1310
AloI	GAACNNNNNNTCC	2 cut(s)	537, 569
Alw21I	GWGCWC	1 cut(s)	306
Alw26I	GTCTC	4 cut(s)	139, 258, 1795, 2388
AlwI	GGATC	5 cut(s)	1049, 2162, 2175, 2538, 2708
AlwNI	CAGNNNCTG	2 cut(s)	1340, 2665
AoxI	GGCC	4 cut(s)	415, 582, 640, 750
ApeKI	GCWGC	7 cut(s)	232, 926, 1334, 1442, 1738, 2662, 2665
ApoI	RAATTY	5 cut(s)	814, 1352, 1718, 1894, 2366
ArsI	GACNNNNNNTTYG	2 cut(s)	1630, 1662
AsiGI	ACCGGT	1 cut(s)	215
Asp700I	GAANNNNTTC	2 cut(s)	1029, 1110
AspLEI	GCGC	1 cut(s)	338
AspS9I	GGNCC	4 cut(s)	583, 890, 1976, 2568
AsuHPI	GGTGA	5 cut(s)	21, 22, 230, 556, 1205
AsuII	TTCGAA	1 cut(s)	2350
AvaII	GGWCC	3 cut(s)	890, 1976, 2568
BalI	TGGCCA	1 cut(s)	752
BamHI	GGATCC	1 cut(s)	2167
BanII	GRGCYC	2 cut(s)	306, 2425
BbsI	GAAGAC	3 cut(s)	320, 2505, 2645
Bbv12I	GWGCWC	1 cut(s)	306
BbvCI	CCTCAGC	1 cut(s)	1881
BbvI	GCAGC	7 cut(s)	244, 913, 1346, 1454, 1725, 2652, 2674
BccI	CCATC	4 cut(s)	857, 928, 1199, 2557
BceAI	ACGGC	3 cut(s)	280, 1076, 2174
BcgI	CGANNNNNNTGC	2 cut(s)	362, 396
BciT130I	CCWGG	3 cut(s)	229, 1822, 2172
BclI	TGATCA	2 cut(s)	258, 387
BcoDI	GTCTC	4 cut(s)	139, 258, 1795, 2388
BcuI	ACTAGT	1 cut(s)	1523
BfaI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 2681
BfmI	CTRYAG	4 cut(s)	320, 894, 1332, 1446
BfrI	CTTAAG	1 cut(s)	2191
BfuAI	ACCTGC	3 cut(s)	901, 1670, 1987
BisI	GCNGC	8 cut(s)	233, 448, 927, 1335, 1443, 1739, 2663, 2666
BlpI	GCTNAGC	1 cut(s)	2316
BlsI	GCNGC	8 cut(s)	234, 449, 928, 1336, 1444, 1740, 2664, 2667
Bme1390I	CCNGG	3 cut(s)	229, 1822, 2172
Bme18I	GGWCC	3 cut(s)	890, 1976, 2568
BmeRI	GACNNNNNGTC	1 cut(s)	1546
BmgBI	CACGTC	1 cut(s)	590
BmgT120I	GGNCC	4 cut(s)	583, 890, 1976, 2568
BmiI	GGNNCC	3 cut(s)	1977, 2169, 2569
BmrFI	CCNGG	3 cut(s)	229, 1822, 2172
BmsI	GCATC	5 cut(s)	1009, 1082, 1280, 1737, 2280
BpiI	GAAGAC	3 cut(s)	320, 2505, 2645
BpmI	CTGGAG	2 cut(s)	510, 1283
Bpu10I	CCTNAGC	2 cut(s)	305, 1881
Bpu1102I	GCTNAGC	1 cut(s)	2316
Bpu14I	TTCGAA	1 cut(s)	2350
BpuEI	CTTGAG	1 cut(s)	829
BsaAI	YACGTR	1 cut(s)	2144
BsaHI	GRCGYC	3 cut(s)	148, 432, 696
BsaI	GGTCTC	1 cut(s)	2388
BsaJI	CCNNGG	5 cut(s)	175, 354, 625, 753, 1702
BsaWI	WCCGGW	2 cut(s)	215, 2697
BsaXI	ACNNNNNCTCC	4 cut(s)	543, 573, 1773, 1803
Bsc4I	CCNNNNNNNGG	3 cut(s)	118, 306, 2007
Bse118I	RCCGGY	2 cut(s)	150, 215
Bse1I	ACTGG	5 cut(s)	493, 547, 1300, 1330, 2158
BseBI	CCWGG	3 cut(s)	229, 1822, 2172
BseDI	CCNNGG	5 cut(s)	175, 354, 625, 753, 1702
BseGI	GGATG	5 cut(s)	849, 979, 1423, 1993, 2485
BseLI	CCNNNNNNNGG	3 cut(s)	118, 306, 2007
BseMII	CTCAG	8 cut(s)	257, 290, 319, 728, 1542, 1806, 1872, 2330
BseNI	ACTGG	5 cut(s)	493, 547, 1300, 1330, 2158
BseRI	GAGGAG	3 cut(s)	418, 812, 1961
BseXI	GCAGC	7 cut(s)	244, 913, 1346, 1454, 1725, 2652, 2674
BseYI	CCCAGC	1 cut(s)	2526
BsgI	GTGCAG	1 cut(s)	179
Bsh1236I	CGCG	2 cut(s)	336, 1062
Bsh1285I	CGRYCG	1 cut(s)	1645
BshFI	GGCC	4 cut(s)	417, 584, 642, 752
BshTI	ACCGGT	1 cut(s)	215
BsiEI	CGRYCG	1 cut(s)	1645
BsiHKAI	GWGCWC	1 cut(s)	306
BsiSI	CCGG	6 cut(s)	99, 119, 142, 151, 216, 2698
BslFI	GGGAC	6 cut(s)	220, 408, 1373, 1989, 2116, 2581
BslI	CCNNNNNNNGG	3 cut(s)	118, 306, 2007
BsmAI	GTCTC	4 cut(s)	139, 258, 1795, 2388
BsmBI	CGTCTC	1 cut(s)	139
BsmFI	GGGAC	6 cut(s)	220, 408, 1373, 1989, 2116, 2581
BsmI	GAATGC	4 cut(s)	538, 1093, 1491, 1717
BsnI	GGCC	4 cut(s)	417, 584, 642, 752
Bso31I	GGTCTC	1 cut(s)	2388
Bsp119I	TTCGAA	1 cut(s)	2350
Bsp1286I	GDGCHC	2 cut(s)	306, 2425
Bsp143I	GATC	8 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2700
Bsp1720I	GCTNAGC	1 cut(s)	2316
BspANI	GGCC	4 cut(s)	417, 584, 642, 752
BspCNI	CTCAG	8 cut(s)	256, 289, 318, 729, 1543, 1805, 1873, 2329
BspFNI	CGCG	2 cut(s)	336, 1062
BspHI	TCATGA	1 cut(s)	2028
BspLI	GGNNCC	3 cut(s)	1977, 2169, 2569
BspMAI	CTGCAG	3 cut(s)	324, 898, 1336
BspMI	ACCTGC	3 cut(s)	901, 1670, 1987
BspPI	GGATC	5 cut(s)	1049, 2162, 2175, 2538, 2708
BspT104I	TTCGAA	1 cut(s)	2350
BspTI	CTTAAG	1 cut(s)	2191
BspTNI	GGTCTC	1 cut(s)	2388
BsrFI	RCCGGY	2 cut(s)	150, 215
BsrI	ACTGG	5 cut(s)	493, 547, 1300, 1330, 2158
BssAI	RCCGGY	2 cut(s)	150, 215
BssECI	CCNNGG	5 cut(s)	175, 354, 625, 753, 1702
BssMI	GATC	8 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2700
BssNAI	GTATAC	1 cut(s)	2406
BssNI	GRCGYC	3 cut(s)	148, 432, 696
BssT1I	CCWWGG	3 cut(s)	354, 625, 1702
Bst1107I	GTATAC	1 cut(s)	2406
Bst2UI	CCWGG	3 cut(s)	229, 1822, 2172
Bst4CI	ACNGT	9 cut(s)	250, 568, 827, 970, 991, 1068, 1110, 1261, 2399
Bst6I	CTCTTC	3 cut(s)	43, 1020, 2721
BstACI	GRCGYC	3 cut(s)	148, 432, 696
BstAFI	CTTAAG	1 cut(s)	2191
BstBAI	YACGTR	1 cut(s)	2144
BstBI	TTCGAA	1 cut(s)	2350
BstC8I	GCNNGC	5 cut(s)	152, 162, 719, 1461, 1853
BstDSI	CCRYGG	1 cut(s)	753
BstEII	GGTNACC	1 cut(s)	2623
BstF5I	GGATG	5 cut(s)	849, 979, 1423, 1993, 2485
BstFNI	CGCG	2 cut(s)	336, 1062
BstHHI	GCGC	1 cut(s)	338
BstKTI	GATC	8 cut(s)	261, 390, 1057, 1925, 2170, 2233, 2533, 2703
BstMAI	GTCTC	4 cut(s)	139, 258, 1795, 2388
BstMBI	GATC	8 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2700
BstMCI	CGRYCG	1 cut(s)	1645
BstMWI	GCNNNNNNNGC	7 cut(s)	147, 935, 1097, 1192, 1226, 1747, 1886
BstNI	CCWGG	3 cut(s)	229, 1822, 2172
BstPI	GGTNACC	1 cut(s)	2623
BstSCI	CCNGG	3 cut(s)	227, 1820, 2170
BstSFI	CTRYAG	4 cut(s)	320, 894, 1332, 1446
BstUI	CGCG	2 cut(s)	336, 1062
BstV1I	GCAGC	7 cut(s)	244, 913, 1346, 1454, 1725, 2652, 2674
BstV2I	GAAGAC	3 cut(s)	320, 2505, 2645
BstX2I	RGATCY	2 cut(s)	2167, 2530
BstYI	RGATCY	2 cut(s)	2167, 2530
BstZ17I	GTATAC	1 cut(s)	2406
BsuRI	GGCC	4 cut(s)	417, 584, 642, 752
BtgI	CCRYGG	1 cut(s)	753
BtrI	CACGTC	1 cut(s)	590
BtsCI	GGATG	5 cut(s)	849, 979, 1423, 1993, 2485
BtsI	GCAGTG	2 cut(s)	171, 2649
BtsIMutI	CAGTG	5 cut(s)	171, 948, 1073, 1800, 2649
BveI	ACCTGC	3 cut(s)	901, 1670, 1987
Cac8I	GCNNGC	5 cut(s)	152, 162, 719, 1461, 1853
CaiI	CAGNNNCTG	2 cut(s)	1340, 2665
CciI	TCATGA	1 cut(s)	2028
CfoI	GCGC	1 cut(s)	338
Cfr10I	RCCGGY	2 cut(s)	150, 215
Cfr13I	GGNCC	4 cut(s)	583, 890, 1976, 2568
CseI	GACGC	2 cut(s)	156, 1940
Csp6I	GTAC	2 cut(s)	238, 1508
CspAI	ACCGGT	1 cut(s)	215
CviAII	CATG	7 cut(s)	53, 557, 1580, 1848, 2029, 2093, 2114
CviQI	GTAC	2 cut(s)	238, 1508
DpnI	GATC	8 cut(s)	260, 389, 1056, 1924, 2169, 2232, 2532, 2702
DpnII	GATC	8 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2700
DriI	GACNNNNNGTC	1 cut(s)	1546
EaeI	YGGCCR	1 cut(s)	750
Eam1104I	CTCTTC	3 cut(s)	43, 1020, 2721
Eam1105I	GACNNNNNGTC	1 cut(s)	1546
EarI	CTCTTC	3 cut(s)	43, 1020, 2721
EciI	GGCGGA	1 cut(s)	1797
Ecl136II	GAGCTC	1 cut(s)	304
Eco130I	CCWWGG	3 cut(s)	354, 625, 1702
Eco147I	AGGCCT	1 cut(s)	417
Eco24I	GRGCYC	2 cut(s)	306, 2425
Eco31I	GGTCTC	1 cut(s)	2388
Eco32I	GATATC	1 cut(s)	2485
Eco47I	GGWCC	3 cut(s)	890, 1976, 2568
Eco53kI	GAGCTC	1 cut(s)	304
Eco57I	CTGAAG	5 cut(s)	924, 1044, 1797, 2642, 2733
Eco91I	GGTNACC	1 cut(s)	2623
EcoICRI	GAGCTC	1 cut(s)	304
EcoO109I	RGGNCCY	1 cut(s)	1976
EcoO65I	GGTNACC	1 cut(s)	2623
EcoRII	CCWGG	3 cut(s)	227, 1820, 2170
EcoRV	GATATC	1 cut(s)	2485
EcoT14I	CCWWGG	3 cut(s)	354, 625, 1702
EcoT22I	ATGCAT	1 cut(s)	538
EcoT38I	GRGCYC	2 cut(s)	306, 2425
ErhI	CCWWGG	3 cut(s)	354, 625, 1702
Esp3I	CGTCTC	1 cut(s)	139
FaeI	CATG	7 cut(s)	56, 560, 1583, 1851, 2032, 2096, 2117
FalI	AAGNNNNNCTT	4 cut(s)	751, 783, 1137, 1169
FaqI	GGGAC	6 cut(s)	220, 408, 1373, 1989, 2116, 2581
FatI	CATG	7 cut(s)	52, 556, 1579, 1847, 2028, 2092, 2113
FauI	CCCGC	4 cut(s)	56, 185, 1067, 1452
FauNDI	CATATG	1 cut(s)	703
FbaI	TGATCA	2 cut(s)	258, 387
FblI	GTMKAC	1 cut(s)	2405
Fnu4HI	GCNGC	8 cut(s)	233, 448, 927, 1335, 1443, 1739, 2663, 2666
FokI	GGATG	5 cut(s)	836, 986, 1430, 2000, 2492
FriOI	GRGCYC	2 cut(s)	306, 2425
Fsp4HI	GCNGC	8 cut(s)	233, 448, 927, 1335, 1443, 1739, 2663, 2666
FspBI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 2681
GlaI	GCGC	1 cut(s)	337
GluI	GCNGC	8 cut(s)	233, 448, 927, 1335, 1443, 1739, 2663, 2666
GsaI	CCCAGC	1 cut(s)	2530
GsuI	CTGGAG	2 cut(s)	510, 1283
HaeIII	GGCC	4 cut(s)	417, 584, 642, 752
HapII	CCGG	6 cut(s)	99, 119, 142, 151, 216, 2698
HgaI	GACGC	2 cut(s)	156, 1940
HhaI	GCGC	1 cut(s)	338
Hin1I	GRCGYC	3 cut(s)	148, 432, 696
Hin1II	CATG	7 cut(s)	56, 560, 1583, 1851, 2032, 2096, 2117
Hin6I	GCGC	1 cut(s)	336
HinP1I	GCGC	1 cut(s)	336
HindIII	AAGCTT	2 cut(s)	287, 1370
HpaII	CCGG	6 cut(s)	99, 119, 142, 151, 216, 2698
HphI	GGTGA	5 cut(s)	21, 22, 230, 556, 1205
Hpy166II	GTNNAC	5 cut(s)	225, 668, 1170, 2180, 2406
Hpy8I	GTNNAC	5 cut(s)	225, 668, 1170, 2180, 2406
Hpy99I	CGWCG	1 cut(s)	591
HpyCH4III	ACNGT	9 cut(s)	250, 568, 827, 970, 991, 1068, 1110, 1261, 2399
HpyCH4IV	ACGT	4 cut(s)	432, 589, 696, 2143
HpyF10VI	GCNNNNNNNGC	7 cut(s)	147, 935, 1097, 1192, 1226, 1747, 1886
HpySE526I	ACGT	4 cut(s)	432, 589, 696, 2143
Hsp92I	GRCGYC	3 cut(s)	148, 432, 696
Hsp92II	CATG	7 cut(s)	56, 560, 1583, 1851, 2032, 2096, 2117
HspAI	GCGC	1 cut(s)	336
KroI	GCCGGC	1 cut(s)	150
KroNI	GCCGGC	1 cut(s)	152
Ksp22I	TGATCA	2 cut(s)	258, 387
Kzo9I	GATC	8 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2700
LmnI	GCTCC	3 cut(s)	799, 1933, 1948
Lsp1109I	GCAGC	7 cut(s)	244, 913, 1346, 1454, 1725, 2652, 2674
LweI	GCATC	5 cut(s)	1009, 1082, 1280, 1737, 2280
MaeI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 2681
MaeII	ACGT	4 cut(s)	432, 589, 696, 2143
MalI	GATC	8 cut(s)	260, 389, 1056, 1924, 2169, 2232, 2532, 2702
MboI	GATC	8 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2700
MfeI	CAATTG	3 cut(s)	1383, 2277, 2357
MflI	RGATCY	2 cut(s)	2167, 2530
MhlI	GDGCHC	2 cut(s)	306, 2425
MlsI	TGGCCA	1 cut(s)	752
MluNI	TGGCCA	1 cut(s)	752
MlyI	GAGTC	9 cut(s)	230, 310, 353, 359, 1430, 1535, 2291, 2519, 2659
MmeI	TCCRAC	4 cut(s)	66, 136, 460, 673
Mox20I	TGGCCA	1 cut(s)	752
Mph1103I	ATGCAT	1 cut(s)	538
MreI	CGCCGGCG	1 cut(s)	150
MroNI	GCCGGC	1 cut(s)	150
MroXI	GAANNNNTTC	2 cut(s)	1029, 1110
MscI	TGGCCA	1 cut(s)	752
MseI	TTAA	6 cut(s)	539, 782, 1374, 1830, 2192, 2246
Msp20I	TGGCCA	1 cut(s)	752
MspA1I	CMGCKG	4 cut(s)	2081, 2320, 2665, 2711
MspCI	CTTAAG	1 cut(s)	2191
MspI	CCGG	6 cut(s)	99, 119, 142, 151, 216, 2698
MspR9I	CCNGG	3 cut(s)	229, 1822, 2172
MunI	CAATTG	3 cut(s)	1383, 2277, 2357
Mva1269I	GAATGC	4 cut(s)	538, 1093, 1491, 1717
MvaI	CCWGG	3 cut(s)	229, 1822, 2172
MvnI	CGCG	2 cut(s)	336, 1062
MwoI	GCNNNNNNNGC	7 cut(s)	147, 935, 1097, 1192, 1226, 1747, 1886
NaeI	GCCGGC	1 cut(s)	152
NdeI	CATATG	1 cut(s)	703
NdeII	GATC	8 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2700
NgoMIV	GCCGGC	1 cut(s)	150
NlaIII	CATG	7 cut(s)	56, 560, 1583, 1851, 2032, 2096, 2117
NlaIV	GGNNCC	3 cut(s)	1977, 2169, 2569
NmeAIII	GCCGAG	1 cut(s)	2150
NmuCI	GTSAC	6 cut(s)	562, 692, 697, 706, 1538, 2601
NsiI	ATGCAT	1 cut(s)	538
NspV	TTCGAA	1 cut(s)	2350
PagI	TCATGA	1 cut(s)	2028
PceI	AGGCCT	1 cut(s)	417
PctI	GAATGC	4 cut(s)	538, 1093, 1491, 1717
PdiI	GCCGGC	1 cut(s)	152
PdmI	GAANNNNTTC	2 cut(s)	1029, 1110
PfeI	GAWTC	6 cut(s)	296, 845, 1030, 1246, 2117, 2429
PflFI	GACNNNGTC	1 cut(s)	250
PfoI	TCCNGGA	2 cut(s)	1820, 2170
PinAI	ACCGGT	1 cut(s)	215
PkrI	GCNGC	8 cut(s)	234, 449, 928, 1336, 1444, 1740, 2664, 2667
PleI	GAGTC	9 cut(s)	229, 310, 353, 358, 1429, 1535, 2290, 2518, 2658
PpsI	GAGTC	9 cut(s)	229, 310, 353, 358, 1429, 1535, 2290, 2518, 2658
Ppu21I	YACGTR	1 cut(s)	2144
PpuMI	RGGWCCY	1 cut(s)	1976
PsiI	TTATAA	2 cut(s)	921, 2453
Psp124BI	GAGCTC	1 cut(s)	306
Psp5II	RGGWCCY	1 cut(s)	1976
Psp6I	CCWGG	3 cut(s)	227, 1820, 2170
PspEI	GGTNACC	1 cut(s)	2623
PspFI	CCCAGC	1 cut(s)	2526
PspGI	CCWGG	3 cut(s)	227, 1820, 2170
PspN4I	GGNNCC	3 cut(s)	1977, 2169, 2569
PspPI	GGNCC	4 cut(s)	583, 890, 1976, 2568
PspPPI	RGGWCCY	1 cut(s)	1976
PstI	CTGCAG	3 cut(s)	324, 898, 1336
PstNI	CAGNNNCTG	2 cut(s)	1340, 2665
PsuI	RGATCY	2 cut(s)	2167, 2530
PsyI	GACNNNGTC	1 cut(s)	250
PvuII	CAGCTG	3 cut(s)	2081, 2665, 2711
RsaI	GTAC	2 cut(s)	239, 1509
RsaNI	GTAC	2 cut(s)	238, 1508
SacI	GAGCTC	1 cut(s)	306
SaqAI	TTAA	6 cut(s)	539, 782, 1374, 1830, 2192, 2246
SatI	GCNGC	8 cut(s)	233, 448, 927, 1335, 1443, 1739, 2663, 2666
Sau3AI	GATC	8 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2700
Sau96I	GGNCC	4 cut(s)	583, 890, 1976, 2568
SchI	GAGTC	9 cut(s)	230, 310, 353, 359, 1430, 1535, 2291, 2519, 2659
ScrFI	CCNGG	3 cut(s)	229, 1822, 2172
SduI	GDGCHC	2 cut(s)	306, 2425
SfaNI	GCATC	5 cut(s)	1009, 1082, 1280, 1737, 2280
SfcI	CTRYAG	4 cut(s)	320, 894, 1332, 1446
SfuI	TTCGAA	1 cut(s)	2350
SgrAI	CRCCGGYG	2 cut(s)	150, 215
SinI	GGWCC	3 cut(s)	890, 1976, 2568
SmlI	CTYRAG	2 cut(s)	808, 2191
SmoI	CTYRAG	2 cut(s)	808, 2191
SpeI	ACTAGT	1 cut(s)	1523
SseBI	AGGCCT	1 cut(s)	417
SspI	AATATT	1 cut(s)	2500
SspMI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 2681
SstI	GAGCTC	1 cut(s)	306
StuI	AGGCCT	1 cut(s)	417
StyD4I	CCNGG	3 cut(s)	227, 1820, 2170
StyI	CCWWGG	3 cut(s)	354, 625, 1702
TaaI	ACNGT	9 cut(s)	250, 568, 827, 970, 991, 1068, 1110, 1261, 2399
TaiI	ACGT	4 cut(s)	435, 592, 699, 2146
TaqI	TCGA	6 cut(s)	137, 401, 1684, 2149, 2343, 2350
TatI	WGTACW	1 cut(s)	1507
TauI	GCSGC	1 cut(s)	450
TfiI	GAWTC	6 cut(s)	296, 845, 1030, 1246, 2117, 2429
Tru1I	TTAA	6 cut(s)	539, 782, 1374, 1830, 2192, 2246
Tru9I	TTAA	6 cut(s)	539, 782, 1374, 1830, 2192, 2246
TscAI	CASTG	5 cut(s)	171, 948, 1073, 1800, 2649
TseFI	GTSAC	6 cut(s)	562, 692, 697, 706, 1538, 2601
TseI	GCWGC	7 cut(s)	232, 926, 1334, 1442, 1738, 2662, 2665
Tsp45I	GTSAC	6 cut(s)	562, 692, 697, 706, 1538, 2601
TspGWI	ACGGA	4 cut(s)	145, 770, 1946, 2423
TspRI	CASTG	5 cut(s)	171, 948, 1073, 1800, 2649
Tth111I	GACNNNGTC	1 cut(s)	250
Vha464I	CTTAAG	1 cut(s)	2191
VpaK11BI	GGWCC	3 cut(s)	890, 1976, 2568
XapI	RAATTY	5 cut(s)	814, 1352, 1718, 1894, 2366
XbaI	TCTAGA	1 cut(s)	1481
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1818
XmiI	GTMKAC	1 cut(s)	2405
XmnI	GAANNNNTTC	2 cut(s)	1029, 1110
XspI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 2681
ZraI	GACGTC	2 cut(s)	433, 697
Zsp2I	ATGCAT	1 cut(s)	538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.