FvH4_1g30190

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002412
Physical Location & Seq
Forward (+)
23858189 .. 23871813
13625 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g30190.t6

Sequence Viewer

Length: 1608 bp
ATGTCTTGCGTGCGGCAAGCAGTAGCTAAGATGATGAGGATGAATAGCCATACAGTTAACCGTTTGCTCTCTCCTTCTTCAAGTGCCGCTCCTTTCTTCTCCGGCGGTGATACTGGCCGTTGGAATCCTCGTCTCTGGTGGTTTCTATCTGGAAATGCAGCACTTTTTGTTGCCATTAATTCGAACACGGTGTTGGCAAAAGATGCATCACTTGATGAGGCTGAGGGGATGGAATTGCGCAGAATCGAGGGTGATTCTGTCAAATTTAACATACATACCTCTAAGTGGAGAGTTTTTACTGATAAGGGCAGGGAATTCTTCCTTCAGGGGAAACTGGAGGAAGCTGAAAAGCTCTTTCTCGCAGCGTTGCAGGAAGCTAAAGAAGGTTTCGGGAAGAGGGACCCGCATGTTGCATCCGCGAGCAACAATCTGGCAGAGCTCTATAGAGTTAATAAGCTATATCATAAGGCAGAACCTTTGTATTTGGAGGCCATCAGCATACTGGAGGAGTCATTTGGTCCTGAAGATATACGAGTTGGGTCTGCCTTCCATAACATGGGGCAGTTCTATATTGTACAGCGGAAGCTGGATGAAGCCCGTGTTTGCTATGAGCATGCTTTGAAGATTAAACGCCGTGTTCTGGGTCTTAACCATACAGATTATGCAGATACTATGTATCATCTCGGAACGGTTCTACACCTCCAAGGAAATGAAAAGGACTCTGAGGCCCTCGTTCAAGATTCCATTCGAATACTCGAGAATGGTGGTCAAGGAGAGTCAATAATGTGCTTAAAGAGAATGCGATATCTTGCTCAGATATATCTGAAATCCAAACGGTTTGAGGAGGCCGAGAAGGTACAAAGAAAGATACTGCATGTAATGGAACTAACAAAGGGATGGAATGTACTGGACACAACAATAACTGCTGAGGGGCTCGCACTGATTCTCCAATCCACTGGGAGCTTAAATGAAGCTGAAGAGCTTTTTGAAAGATGTCTTGATGCTCGAAAAGCCTTACTTCCACGAGATCACATTCAGATTGGTGTGAACTTGCTTCATATTGCTAGAGTGGCAATGCTTAATTCTGACCAACTGAAGAATTTTGACTATCCAAAAGCAATTTCTGAGCTTGATAAGGCCAATGAAGCGTTACACGAGTCCATAAGGATAGCACAATGTTGCTTGGATAAATTGAAAAAGCAAAATAAGAAGATTCACAATTTTGGAGCAACAGGTGAAATTGGAAAGAATGCTCAGGTGCCGTTGGTCATATTGTTGGAAGCTTCTGATGCTCTTGGCCTTTTAGAGATTACTAAGAAACAATTACAAGGATCCAAGCAGTCTGAACGTGCACCTAATATTGAAGCTGAGAATGCACTATTTAAAGGTGTATCCGCTTATAAAGAGTTTGTAACTGAGAGATCAATCACTGAGTCTCCTGAAATGAAGGTCGAGTATCTTAAGTGTTTGAAGCATCTTTTGAGCTTGAATAGTGATGGCACTACAGAAAGAACACAGCAATCTACAGAAGCCAATTTGCAAGATCTGAGAGACGAAATTAAGCGCCTTGAAGTTGAACTTTCTCCTCATAGGAAACACAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

536

Amino Acids

60.56

Weight (kDa)

7.66

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 107 - 170 9.3e-09 Tetratricopeptide repeat
TPR_12 PF13424 137 - 188 5.3e-07 Tetratricopeptide repeat
TPR_12 PF13424 180 - 253 2.6e-14 Tetratricopeptide repeat
TPR_10 PF13374 181 - 220 1.5e-07 Tetratricopeptide repeat
TPR_10 PF13374 284 - 319 1e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1401
Acc16I TGCGCA 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 1258
AccB7I CCANNNNNTGG 1 cut(s) 556
AccBSI CCGCTC 1 cut(s) 89
AccII CGCG 1 cut(s) 419
AciI CCGC 7 cut(s) 13, 87, 105, 404, 417, 580, 1395
AclWI GGATC 2 cut(s) 1326, 1339
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 4 cut(s) 263, 314, 1099, 1602
AcuI CTGAAG 4 cut(s) 308, 543, 996, 1115
AfaI GTAC 3 cut(s) 576, 858, 906
AfiI CCNNNNNNNGG 3 cut(s) 285, 556, 640
AflII CTTAAG 1 cut(s) 1460
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 1105, 1137
Alw21I GWGCWC 2 cut(s) 441, 1354
Alw26I GTCTC 3 cut(s) 137, 1440, 1545
Alw44I GTGCAC 1 cut(s) 1350
AlwI GGATC 2 cut(s) 1326, 1339
Ama87I CYCGRG 1 cut(s) 755
AoxI GGCC 6 cut(s) 115, 489, 726, 846, 1137, 1297
ApaLI GTGCAC 1 cut(s) 1350
ApeKI GCWGC 2 cut(s) 158, 362
ApoI RAATTY 4 cut(s) 263, 314, 1099, 1602
AseI ATTAAT 1 cut(s) 177
AspLEI GCGC 2 cut(s) 240, 1566
AspS9I GGNCC 3 cut(s) 400, 518, 727
AsuHPI GGTGA 3 cut(s) 119, 263, 1247
AsuII TTCGAA 2 cut(s) 182, 748
AvaI CYCGRG 1 cut(s) 755
AvaII GGWCC 2 cut(s) 400, 518
BaeGI GKGCMC 1 cut(s) 1354
BamHI GGATCC 1 cut(s) 1331
BanI GGYRCC 1 cut(s) 1258
BanII GRGCYC 2 cut(s) 441, 936
BauI CACGAG 2 cut(s) 1023, 1154
Bbv12I GWGCWC 2 cut(s) 441, 1354
BbvCI CCTCAGC 2 cut(s) 222, 927
BbvI GCAGC 2 cut(s) 170, 374
BccI CCATC 4 cut(s) 223, 500, 891, 1490
BceAI ACGGC 3 cut(s) 102, 618, 1246
BciVI GTATCC 1 cut(s) 1402
BcoDI GTCTC 3 cut(s) 137, 1440, 1545
BfaI CTAG 1 cut(s) 1065
BfmI CTRYAG 3 cut(s) 442, 1503, 1524
BfoI RGCGCY 1 cut(s) 1567
BfrI CTTAAG 1 cut(s) 1460
BfuI GTATCC 1 cut(s) 1402
BglII AGATCT 1 cut(s) 1543
BisI GCNGC 4 cut(s) 14, 87, 159, 363
BlsI GCNGC 4 cut(s) 15, 88, 160, 364
Bme18I GGWCC 2 cut(s) 400, 518
BmeT110I CYCGRG 1 cut(s) 755
BmgT120I GGNCC 3 cut(s) 400, 518, 727
BmiI GGNNCC 4 cut(s) 401, 402, 1260, 1333
BmrI ACTGGG 1 cut(s) 966
BmsI GCATC 6 cut(s) 193, 215, 422, 991, 1279, 1483
BmuI ACTGGG 1 cut(s) 966
BpmI CTGGAG 2 cut(s) 356, 524
Bpu10I CCTNAGC 3 cut(s) 222, 927, 1254
Bpu14I TTCGAA 2 cut(s) 182, 748
BsaJI CCNNGG 1 cut(s) 703
BsaXI ACNNNNNCTCC 4 cut(s) 930, 960, 1420, 1450
Bsc4I CCNNNNNNNGG 3 cut(s) 285, 556, 640
Bse1I ACTGG 5 cut(s) 118, 339, 507, 912, 961
Bse3DI GCAATG 1 cut(s) 1080
BseDI CCNNGG 1 cut(s) 703
BseGI GGATG 5 cut(s) 45, 234, 413, 595, 902
BseLI CCNNNNNNNGG 3 cut(s) 285, 556, 640
BseMI GCAATG 1 cut(s) 1080
BseNI ACTGG 5 cut(s) 118, 339, 507, 912, 961
BseRI GAGGAG 3 cut(s) 521, 857, 1575
BseSI GKGCMC 1 cut(s) 1354
BseXI GCAGC 2 cut(s) 170, 374
Bsh1236I CGCG 1 cut(s) 419
BshFI GGCC 6 cut(s) 117, 491, 728, 848, 1139, 1299
BshNI GGYRCC 1 cut(s) 1258
BsiHKAI GWGCWC 2 cut(s) 441, 1354
BsiHKCI CYCGRG 1 cut(s) 755
BsiSI CCGG 1 cut(s) 102
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 3 cut(s) 285, 556, 640
BsmAI GTCTC 3 cut(s) 137, 1440, 1545
BsmBI CGTCTC 2 cut(s) 137, 1545
BsmFI GGGAC 1 cut(s) 413
BsmI GAATGC 3 cut(s) 804, 1255, 1378
BsnI GGCC 6 cut(s) 117, 491, 728, 848, 1139, 1299
BsoBI CYCGRG 1 cut(s) 755
Bsp119I TTCGAA 2 cut(s) 182, 748
Bsp1286I GDGCHC 3 cut(s) 441, 936, 1354
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 4 cut(s) 1027, 1331, 1421, 1543
BspACI CCGC 7 cut(s) 13, 87, 105, 404, 417, 580, 1395
BspANI GGCC 6 cut(s) 117, 491, 728, 848, 1139, 1299
BspFNI CGCG 1 cut(s) 419
BspLI GGNNCC 4 cut(s) 401, 402, 1260, 1333
BspPI GGATC 2 cut(s) 1326, 1339
BspQI GCTCTTC 1 cut(s) 972
BspT104I TTCGAA 2 cut(s) 182, 748
BspT107I GGYRCC 1 cut(s) 1258
BspTI CTTAAG 1 cut(s) 1460
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 1080
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 5 cut(s) 118, 339, 507, 912, 961
BssECI CCNNGG 1 cut(s) 703
BssMI GATC 4 cut(s) 1027, 1331, 1421, 1543
BssSI CACGAG 2 cut(s) 1023, 1154
BssT1I CCWWGG 1 cut(s) 703
Bst2BI CACGAG 2 cut(s) 1023, 1154
Bst4CI ACNGT 5 cut(s) 55, 62, 190, 691, 837
Bst6I CTCTTC 2 cut(s) 389, 972
BstAFI CTTAAG 1 cut(s) 1460
BstAPI GCANNNNNTGC 1 cut(s) 203
BstAUI TGTACA 1 cut(s) 574
BstBI TTCGAA 2 cut(s) 182, 748
BstC8I GCNNGC 5 cut(s) 11, 18, 421, 615, 936
BstF5I GGATG 5 cut(s) 45, 234, 413, 595, 902
BstFNI CGCG 1 cut(s) 419
BstH2I RGCGCY 1 cut(s) 1567
BstHHI GCGC 2 cut(s) 240, 1566
BstKTI GATC 4 cut(s) 1030, 1334, 1424, 1546
BstMAI GTCTC 3 cut(s) 137, 1440, 1545
BstMBI GATC 4 cut(s) 1027, 1331, 1421, 1543
BstMWI GCNNNNNNNGC 6 cut(s) 203, 1010, 1070, 1145, 1289, 1373
BstNSI RCATGY 3 cut(s) 410, 617, 878
BstSFI CTRYAG 3 cut(s) 442, 1503, 1524
BstSLI GKGCMC 1 cut(s) 1354
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 2 cut(s) 170, 374
BstX2I RGATCY 2 cut(s) 1331, 1543
BstXI CCANNNNNNTGG 1 cut(s) 956
BstYI RGATCY 2 cut(s) 1331, 1543
BsuI GTATCC 1 cut(s) 1402
BsuRI GGCC 6 cut(s) 117, 491, 728, 848, 1139, 1299
BtsCI GGATG 5 cut(s) 45, 234, 413, 595, 902
BtsIMutI CAGTG 3 cut(s) 938, 954, 1428
Cac8I GCNNGC 5 cut(s) 11, 18, 421, 615, 936
CfoI GCGC 2 cut(s) 240, 1566
Cfr13I GGNCC 3 cut(s) 400, 518, 727
Csp6I GTAC 3 cut(s) 575, 857, 905
CviAII CATG 4 cut(s) 407, 556, 614, 875
CviQI GTAC 3 cut(s) 575, 857, 905
DpnI GATC 4 cut(s) 1029, 1333, 1423, 1545
DpnII GATC 4 cut(s) 1027, 1331, 1421, 1543
DraI TTTAAA 1 cut(s) 1384
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 2 cut(s) 389, 972
EarI CTCTTC 2 cut(s) 389, 972
Ecl136II GAGCTC 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 703
Eco24I GRGCYC 2 cut(s) 441, 936
Eco32I GATATC 1 cut(s) 806
Eco47I GGWCC 2 cut(s) 400, 518
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 4 cut(s) 308, 543, 996, 1115
Eco88I CYCGRG 1 cut(s) 755
EcoICRI GAGCTC 1 cut(s) 439
EcoO109I RGGNCCY 2 cut(s) 400, 727
EcoRI GAATTC 1 cut(s) 314
EcoRV GATATC 1 cut(s) 806
EcoT14I CCWWGG 1 cut(s) 703
EcoT22I ATGCAT 1 cut(s) 208
EcoT38I GRGCYC 2 cut(s) 441, 936
ErhI CCWWGG 1 cut(s) 703
Esp3I CGTCTC 2 cut(s) 137, 1545
FaeI CATG 4 cut(s) 410, 559, 617, 878
FalI AAGNNNNNCTT 4 cut(s) 1002, 1034, 1563, 1595
FaqI GGGAC 1 cut(s) 413
FatI CATG 4 cut(s) 406, 555, 613, 874
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 4 cut(s) 14, 87, 159, 363
FokI GGATG 5 cut(s) 52, 241, 400, 602, 909
FriOI GRGCYC 2 cut(s) 441, 936
Fsp4HI GCNGC 4 cut(s) 14, 87, 159, 363
FspBI CTAG 1 cut(s) 1065
FspI TGCGCA 1 cut(s) 239
GlaI GCGC 2 cut(s) 239, 1565
GluI GCNGC 4 cut(s) 14, 87, 159, 363
GsuI CTGGAG 2 cut(s) 356, 524
HaeII RGCGCY 1 cut(s) 1567
HaeIII GGCC 6 cut(s) 117, 491, 728, 848, 1139, 1299
HapII CCGG 1 cut(s) 102
HhaI GCGC 2 cut(s) 240, 1566
Hin1II CATG 4 cut(s) 410, 559, 617, 878
Hin6I GCGC 2 cut(s) 238, 1564
HinP1I GCGC 2 cut(s) 238, 1564
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 1 cut(s) 1281
HpaI GTTAAC 1 cut(s) 58
HpaII CCGG 1 cut(s) 102
HphI GGTGA 3 cut(s) 119, 263, 1247
Hpy166II GTNNAC 3 cut(s) 58, 1048, 1352
Hpy188III TCNNGA 7 cut(s) 150, 391, 521, 737, 757, 998, 1439
Hpy8I GTNNAC 3 cut(s) 58, 1048, 1352
HpyAV CCTTC 6 cut(s) 84, 332, 377, 556, 847, 1441
HpyCH4III ACNGT 5 cut(s) 55, 62, 190, 691, 837
HpyCH4IV ACGT 1 cut(s) 1348
HpyCH4V TGCA 9 cut(s) 158, 206, 370, 413, 665, 874, 1352, 1376, 1540
HpyF10VI GCNNNNNNNGC 6 cut(s) 203, 1010, 1070, 1145, 1289, 1373
HpySE526I ACGT 1 cut(s) 1348
Hsp92II CATG 4 cut(s) 410, 559, 617, 878
HspAI GCGC 2 cut(s) 238, 1564
KflI GGGWCCC 1 cut(s) 400
KspAI GTTAAC 1 cut(s) 58
Kzo9I GATC 4 cut(s) 1027, 1331, 1421, 1543
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 3 cut(s) 94, 960, 1226
Lsp1109I GCAGC 2 cut(s) 170, 374
LweI GCATC 6 cut(s) 193, 215, 422, 991, 1279, 1483
MaeI CTAG 1 cut(s) 1065
MaeII ACGT 1 cut(s) 1348
MaeIII GTNAC 2 cut(s) 1149, 1411
MalI GATC 4 cut(s) 1029, 1333, 1423, 1545
MbiI CCGCTC 1 cut(s) 89
MboI GATC 4 cut(s) 1027, 1331, 1421, 1543
MboII GAAGA 9 cut(s) 69, 88, 310, 406, 536, 634, 989, 1108, 1222
MflI RGATCY 2 cut(s) 1331, 1543
MhlI GDGCHC 3 cut(s) 441, 936, 1354
MlyI GAGTC 5 cut(s) 518, 713, 785, 1166, 1442
MmeI TCCRAC 2 cut(s) 101, 1257
Mph1103I ATGCAT 1 cut(s) 208
MspA1I CMGCKG 1 cut(s) 580
MspCI CTTAAG 1 cut(s) 1460
MspI CCGG 1 cut(s) 102
Mva1269I GAATGC 3 cut(s) 804, 1255, 1378
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 6 cut(s) 203, 1010, 1070, 1145, 1289, 1373
NdeII GATC 4 cut(s) 1027, 1331, 1421, 1543
NlaIII CATG 4 cut(s) 410, 559, 617, 878
NlaIV GGNNCC 4 cut(s) 401, 402, 1260, 1333
NmeAIII GCCGAG 1 cut(s) 874
NsbI TGCGCA 1 cut(s) 239
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 3 cut(s) 410, 617, 878
NspV TTCGAA 2 cut(s) 182, 748
PaeI GCATGC 1 cut(s) 617
PaeR7I CTCGAG 1 cut(s) 755
PciSI GCTCTTC 1 cut(s) 972
PctI GAATGC 3 cut(s) 804, 1255, 1378
PfeI GAWTC 6 cut(s) 124, 243, 254, 740, 943, 1213
PflMI CCANNNNNTGG 1 cut(s) 556
PkrI GCNGC 4 cut(s) 15, 88, 160, 364
PleI GAGTC 5 cut(s) 517, 713, 784, 1165, 1441
PpsI GAGTC 5 cut(s) 517, 713, 784, 1165, 1441
PpuMI RGGWCCY 1 cut(s) 400
PshBI ATTAAT 1 cut(s) 177
PsiI TTATAA 1 cut(s) 1401
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 400
PspN4I GGNNCC 4 cut(s) 401, 402, 1260, 1333
PspPI GGNCC 3 cut(s) 400, 518, 727
PspPPI RGGWCCY 1 cut(s) 400
PsuI RGATCY 2 cut(s) 1331, 1543
RsaI GTAC 3 cut(s) 576, 858, 906
RsaNI GTAC 3 cut(s) 575, 857, 905
SacI GAGCTC 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 972
SatI GCNGC 4 cut(s) 14, 87, 159, 363
Sau3AI GATC 4 cut(s) 1027, 1331, 1421, 1543
Sau96I GGNCC 3 cut(s) 400, 518, 727
SchI GAGTC 5 cut(s) 518, 713, 785, 1166, 1442
SduI GDGCHC 3 cut(s) 441, 936, 1354
SfaNI GCATC 6 cut(s) 193, 215, 422, 991, 1279, 1483
SfcI CTRYAG 3 cut(s) 442, 1503, 1524
Sfr274I CTCGAG 1 cut(s) 755
SfuI TTCGAA 2 cut(s) 182, 748
SinI GGWCC 2 cut(s) 400, 518
SlaI CTCGAG 1 cut(s) 755
SmlI CTYRAG 2 cut(s) 755, 1460
SmoI CTYRAG 2 cut(s) 755, 1460
SphI GCATGC 1 cut(s) 617
SsiI CCGC 7 cut(s) 13, 87, 105, 404, 417, 580, 1395
SspI AATATT 1 cut(s) 1360
SspMI CTAG 1 cut(s) 1065
SstI GAGCTC 1 cut(s) 441
StyI CCWWGG 1 cut(s) 703
TaaI ACNGT 5 cut(s) 55, 62, 190, 691, 837
TaiI ACGT 1 cut(s) 1351
TaqI TCGA 6 cut(s) 182, 246, 748, 756, 1006, 1452
TatI WGTACW 2 cut(s) 574, 904
TauI GCSGC 2 cut(s) 16, 89
TfiI GAWTC 6 cut(s) 124, 243, 254, 740, 943, 1213
TscAI CASTG 3 cut(s) 945, 961, 1435
TseI GCWGC 2 cut(s) 158, 362
TspDTI ATGAA 7 cut(s) 56, 606, 726, 984, 1046, 1158, 1460
TspRI CASTG 3 cut(s) 945, 961, 1435
Van91I CCANNNNNTGG 1 cut(s) 556
Vha464I CTTAAG 1 cut(s) 1460
VneI GTGCAC 1 cut(s) 1350
VpaK11BI GGWCC 2 cut(s) 400, 518
VspI ATTAAT 1 cut(s) 177
XapI RAATTY 4 cut(s) 263, 314, 1099, 1602
XceI RCATGY 3 cut(s) 410, 617, 878
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XhoI CTCGAG 1 cut(s) 755
XspI CTAG 1 cut(s) 1065
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.