MD17G1268600.v1.1

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002412
Physical Location & Seq
Forward (+)
32823434 .. 32832868
9435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1268600.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGTCTCTGCGACAAGCAGCAGCTAACATGATCAAGCGGCGTTTCCGTTTGGCCTCCCAAACTTTCTCTTCAACTTGCGGCGGAGCAGCCACTGTTATTCCACTCGCCAGGCGTACCAGATTTGATCACGGTTCTAAGGATTGTAGTTGGAGGATGAATTATAGCCACAGGGACCCTCGTCTGTGGCTTTTTGTATCCGGCAATGCAGCAATTTTTCTTGCGATACATTCCAACACTGTCTTAGCAAAAGATGAATCAGTTGATCCTGATAATGGCACAGAAGGAGCTAAAGCTATGGGATTGCGCAAAATTGAGGACGGTTCTGTGATATCAAATATACATACTTCTAAGTGGAGAATTTTTACTGATAAAGGGAGGGATTTTTTCCTTCAGGCAAGGATTATATGCTATGTGCCTTCACTTGTTTGCTATGGAAAATTGGAGGAAGCAGAAAAGCTTTTCCTCTCAGCTTTACAAGAAGCGAAAGAAGGCTTTGGGGAAAGGGAACCACATGTTGCATCTGCATGCAACAATCTAGCAGAGCTCTATAGGGTTAATAAGGCATATAATAAAGCAGAGCCCTTGTACTTGGAAGCTATCAGCATACTGGAGGAATCATTTGGTCCTGAAGATATAAGAGTTGGGGTTGCCTTTCACAACCTTGGCCAGTTCTACATTGCACAACGGAAGCTTGAAGAAGCTCGCACTTGCTATGAGCGTGCTTTGAAGATTAAGGGCTGCGTTTTGGGTCTTAACCATTCAGATTATGCAGATACTATGTATCATCTTGGAACGGTGCTACACCTCCAAGGAAAAGAAAAGGATTCTGAGGCCCTTATTCAGGATTCGATCAGAATACTTGAGGATGGTGGCCTAGGGGAGTCAATGGTGTGTGTCAAGAGAATGCGATCTCTTGCTCAGATATATACAAAATCCAATCGTTCTGCAGAGGCTGAGAACGTACAAAGAAAGATTCTACATATAATGGAATTAACGAAGGGATGGAATTCAATGGAAACAGTTGTAACAGCTGAGGGGCTCGCACTGATCCTACAATCCGCCGGGAGCTTAAAGGATGCTGAAGAGCTTCTTGAAAGATGTCTTGATGCTCGGAAAACCTTACTTCCTGAAGATCATATCCAGATTGGTGCAAATATGCTTCACATTGCTAGAGTGGCAATGCTCAATTCCAACCAACTAAAGAAGATGGACACTTCTAAAGCAATTTCTGAGCTTGACAAGGCCAGAGATAATTTAAATGAGTCCATAAGGATAGCACGGTGTTCGTTGGATAAATCAATCAAGAAGAATAGAAAGATACAAAGTGATATTGGAAGGAGTTGTCGTGTGCCTTTGGTCATAATGTTGCAAGCATTTGACGCTCTTAGCCTTGTGGCGATCGCTAAGCAAGAATTACAGGAATCAAAGGATGAGTGTTCACCTAACATCGAAGCTGAGGATGCACTATTTAGATGCATCTCTGCTTACAAAGAGTTTGTAACTGAGAAATCGGTTGCTGAGTTTCCTGAAGTGAAGGCAGAGTATCTTAAATGTTTGAAGCATCTTATAAGCTTAAATAGTGGTACCAGTACCCAAAGGTCACAGCAATCTACAAGAGTCAATCTGCAAGATCTGAGAGATGAAATCAAGCATCTTGAACTCGAACTCTCCCCGTCTAGGAAACGTAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

565

Amino Acids

63.45

Weight (kDa)

8.5

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 145 - 205 3.1e-06 Tetratricopeptide repeat
TPR_10 PF13374 172 - 211 3.8e-06 Tetratricopeptide repeat
TPR_12 PF13424 174 - 244 8.6e-13 Tetratricopeptide repeat
TPR_12 PF13424 216 - 288 3.2e-12 Tetratricopeptide repeat
TPR_10 PF13374 217 - 255 1e-05 Tetratricopeptide repeat
TPR_NPHP3 PF24885 217 - 332 4.9e-09 Nephrocystin-3 TPR domain
TPR_12 PF13424 304 - 372 3.9e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1568
Acc16I TGCGCA 1 cut(s) 305
Acc65I GGTACC 1 cut(s) 1583
AccB1I GGYRCC 1 cut(s) 1583
AciI CCGC 4 cut(s) 37, 78, 81, 1059
AclWI GGATC 2 cut(s) 257, 1042
AcoI YGGCCR 1 cut(s) 664
AcsI RAATTY 2 cut(s) 357, 1006
AcuI CTGAAG 5 cut(s) 374, 648, 1101, 1149, 1548
AfaI GTAC 5 cut(s) 115, 587, 963, 1585, 1591
AfiI CCNNNNNNNGG 3 cut(s) 272, 841, 1677
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 7 cut(s) 72, 695, 727, 1011, 1094, 1558, 1658
AjnI CCWGG 1 cut(s) 107
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 257, 1042
AlwNI CAGNNNCTG 2 cut(s) 92, 953
AoxI GGCC 5 cut(s) 51, 664, 831, 871, 1242
ApeKI GCWGC 5 cut(s) 17, 20, 86, 206, 738
ApoI RAATTY 2 cut(s) 357, 1006
AsiSI GCGATCGC 1 cut(s) 1401
Asp700I GAANNNNTTC 1 cut(s) 1086
Asp718I GGTACC 1 cut(s) 1583
AspA2I CCTAGG 1 cut(s) 874
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 3 cut(s) 172, 623, 832
AsuC2I CCSGG 1 cut(s) 1063
AsuHPI GGTGA 1 cut(s) 1431
AvaII GGWCC 2 cut(s) 172, 623
AvrII CCTAGG 1 cut(s) 874
BalI TGGCCA 1 cut(s) 666
BanI GGYRCC 1 cut(s) 1583
BanII GRGCYC 3 cut(s) 546, 582, 1041
Bbv12I GWGCWC 1 cut(s) 546
BbvCI CCTCAGC 2 cut(s) 1032, 1455
BbvI GCAGC 5 cut(s) 29, 32, 98, 218, 725
BccI CCATC 3 cut(s) 860, 996, 1201
BcgI CGANNNNNNTGC 2 cut(s) 1266, 1300
BciT130I CCWGG 1 cut(s) 109
BciVI GTATCC 1 cut(s) 205
BclI TGATCA 2 cut(s) 30, 124
BcnI CCSGG 1 cut(s) 1063
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 4 cut(s) 536, 875, 1170, 1677
BfmI CTRYAG 2 cut(s) 547, 945
BfuI GTATCC 1 cut(s) 205
BglII AGATCT 1 cut(s) 1630
BisI GCNGC 7 cut(s) 18, 21, 38, 79, 87, 207, 739
BlnI CCTAGG 1 cut(s) 874
BlpI GCTNAGC 1 cut(s) 1404
BlsI GCNGC 7 cut(s) 19, 22, 39, 80, 88, 208, 740
Bme1390I CCNGG 2 cut(s) 109, 1063
Bme18I GGWCC 2 cut(s) 172, 623
BmgT120I GGNCC 3 cut(s) 172, 623, 832
BmiI GGNNCC 4 cut(s) 173, 174, 507, 1585
BmrFI CCNGG 2 cut(s) 109, 1063
BmsI GCATC 8 cut(s) 527, 1066, 1096, 1450, 1463, 1485, 1570, 1660
BoxI GACNNNNGTC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 2 cut(s) 1032, 1455
Bpu1102I GCTNAGC 1 cut(s) 1404
BpuEI CTTGAG 1 cut(s) 881
BpuMI CCSGG 1 cut(s) 1063
BsaJI CCNNGG 3 cut(s) 661, 808, 874
BsaXI ACNNNNNCTCC 2 cut(s) 1330, 1360
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 841, 1677
Bse1I ACTGG 3 cut(s) 612, 667, 1587
Bse3DI GCAATG 4 cut(s) 208, 675, 1164, 1185
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 3 cut(s) 661, 808, 874
BseGI GGATG 6 cut(s) 159, 871, 1007, 1081, 1435, 1465
BseLI CCNNNNNNNGG 3 cut(s) 272, 841, 1677
BseMI GCAATG 4 cut(s) 208, 675, 1164, 1185
BseNI ACTGG 3 cut(s) 612, 667, 1587
BseXI GCAGC 5 cut(s) 29, 32, 98, 218, 725
Bsh1285I CGRYCG 1 cut(s) 1401
BshFI GGCC 5 cut(s) 53, 666, 833, 873, 1244
BshNI GGYRCC 1 cut(s) 1583
BsiEI CGRYCG 1 cut(s) 1401
BsiHKAI GWGCWC 1 cut(s) 546
BsiSI CCGG 2 cut(s) 198, 1062
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 3 cut(s) 272, 841, 1677
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 909
BsnI GGCC 5 cut(s) 53, 666, 833, 873, 1244
Bsp1286I GDGCHC 3 cut(s) 546, 582, 1041
Bsp143I GATC 9 cut(s) 30, 124, 262, 849, 908, 1047, 1132, 1398, 1630
Bsp1720I GCTNAGC 1 cut(s) 1404
BspACI CCGC 4 cut(s) 37, 78, 81, 1059
BspANI GGCC 5 cut(s) 53, 666, 833, 873, 1244
BspLI GGNNCC 4 cut(s) 173, 174, 507, 1585
BspMAI CTGCAG 1 cut(s) 949
BspPI GGATC 2 cut(s) 257, 1042
BspQI GCTCTTC 1 cut(s) 1077
BspT107I GGYRCC 1 cut(s) 1583
BsrDI GCAATG 4 cut(s) 208, 675, 1164, 1185
BsrI ACTGG 3 cut(s) 612, 667, 1587
BssECI CCNNGG 3 cut(s) 661, 808, 874
BssMI GATC 9 cut(s) 30, 124, 262, 849, 908, 1047, 1132, 1398, 1630
BssT1I CCWWGG 3 cut(s) 661, 808, 874
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 7 cut(s) 94, 131, 238, 320, 796, 1021, 1281
Bst6I CTCTTC 2 cut(s) 73, 1077
BstC8I GCNNGC 5 cut(s) 526, 703, 720, 1041, 1371
BstENI CCTNNNNNAGG 1 cut(s) 839
BstF5I GGATG 6 cut(s) 159, 871, 1007, 1081, 1435, 1465
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 9 cut(s) 33, 127, 265, 852, 911, 1050, 1135, 1401, 1633
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 9 cut(s) 30, 124, 262, 849, 908, 1047, 1132, 1398, 1630
BstMCI CGRYCG 1 cut(s) 1401
BstMWI GCNNNNNNNGC 3 cut(s) 1175, 1379, 1460
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 2 cut(s) 515, 528
BstPAI GACNNNNGTC 1 cut(s) 177
BstSCI CCNGG 2 cut(s) 107, 1061
BstSFI CTRYAG 2 cut(s) 547, 945
BstV1I GCAGC 5 cut(s) 29, 32, 98, 218, 725
BstX2I RGATCY 1 cut(s) 1630
BstYI RGATCY 1 cut(s) 1630
BsuI GTATCC 1 cut(s) 205
BsuRI GGCC 5 cut(s) 53, 666, 833, 873, 1244
BtsCI GGATG 6 cut(s) 159, 871, 1007, 1081, 1435, 1465
BtsIMutI CAGTG 3 cut(s) 90, 234, 1043
Cac8I GCNNGC 5 cut(s) 526, 703, 720, 1041, 1371
CaiI CAGNNNCTG 2 cut(s) 92, 953
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 3 cut(s) 172, 623, 832
CseI GACGC 1 cut(s) 1388
Csp6I GTAC 5 cut(s) 114, 586, 962, 1584, 1590
CviAII CATG 3 cut(s) 28, 512, 525
CviQI GTAC 5 cut(s) 114, 586, 962, 1584, 1590
DpnI GATC 9 cut(s) 32, 126, 264, 851, 910, 1049, 1134, 1400, 1632
DpnII GATC 9 cut(s) 30, 124, 262, 849, 908, 1047, 1132, 1398, 1630
DraI TTTAAA 1 cut(s) 1257
EaeI YGGCCR 1 cut(s) 664
Eam1104I CTCTTC 2 cut(s) 73, 1077
EarI CTCTTC 2 cut(s) 73, 1077
EciI GGCGGA 2 cut(s) 96, 1048
Ecl136II GAGCTC 1 cut(s) 544
Eco130I CCWWGG 3 cut(s) 661, 808, 874
Eco24I GRGCYC 3 cut(s) 546, 582, 1041
Eco32I GATATC 1 cut(s) 330
Eco47I GGWCC 2 cut(s) 172, 623
Eco53kI GAGCTC 1 cut(s) 544
Eco57I CTGAAG 5 cut(s) 374, 648, 1101, 1149, 1548
EcoICRI GAGCTC 1 cut(s) 544
EcoNI CCTNNNNNAGG 1 cut(s) 839
EcoO109I RGGNCCY 2 cut(s) 172, 832
EcoRI GAATTC 1 cut(s) 1006
EcoRII CCWGG 1 cut(s) 107
EcoRV GATATC 1 cut(s) 330
EcoT14I CCWWGG 3 cut(s) 661, 808, 874
EcoT22I ATGCAT 1 cut(s) 1478
EcoT38I GRGCYC 3 cut(s) 546, 582, 1041
ErhI CCWWGG 3 cut(s) 661, 808, 874
FaeI CATG 3 cut(s) 31, 515, 528
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FaqI GGGAC 1 cut(s) 185
FatI CATG 3 cut(s) 27, 511, 524
FbaI TGATCA 2 cut(s) 30, 124
Fnu4HI GCNGC 7 cut(s) 18, 21, 38, 79, 87, 207, 739
FokI GGATG 6 cut(s) 166, 878, 1014, 1088, 1442, 1472
FriOI GRGCYC 3 cut(s) 546, 582, 1041
Fsp4HI GCNGC 7 cut(s) 18, 21, 38, 79, 87, 207, 739
FspBI CTAG 4 cut(s) 536, 875, 1170, 1677
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 1 cut(s) 305
GluI GCNGC 7 cut(s) 18, 21, 38, 79, 87, 207, 739
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 5 cut(s) 53, 666, 833, 873, 1244
HapII CCGG 2 cut(s) 198, 1062
HgaI GACGC 1 cut(s) 1388
HhaI GCGC 1 cut(s) 306
Hin1II CATG 3 cut(s) 31, 515, 528
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HindIII AAGCTT 3 cut(s) 455, 689, 1570
HinfI GANTC 9 cut(s) 254, 614, 824, 845, 881, 973, 1262, 1421, 1617
HpaII CCGG 2 cut(s) 198, 1062
HphI GGTGA 1 cut(s) 1431
Hpy166II GTNNAC 1 cut(s) 1439
Hpy188I TCNGA 7 cut(s) 763, 829, 854, 921, 1113, 1231, 1635
Hpy8I GTNNAC 1 cut(s) 1439
HpyAV CCTTC 7 cut(s) 275, 398, 426, 482, 991, 1329, 1528
HpyCH4III ACNGT 7 cut(s) 94, 131, 238, 320, 796, 1021, 1281
HpyCH4IV ACGT 2 cut(s) 960, 1684
HpyF10VI GCNNNNNNNGC 3 cut(s) 1175, 1379, 1460
HpySE526I ACGT 2 cut(s) 960, 1684
Hsp92II CATG 3 cut(s) 31, 515, 528
HspAI GCGC 1 cut(s) 304
KflI GGGWCCC 1 cut(s) 172
KpnI GGTACC 1 cut(s) 1587
Ksp22I TGATCA 2 cut(s) 30, 124
Kzo9I GATC 9 cut(s) 30, 124, 262, 849, 908, 1047, 1132, 1398, 1630
LguI GCTCTTC 1 cut(s) 1077
LmnI GCTCC 3 cut(s) 83, 284, 1065
Lsp1109I GCAGC 5 cut(s) 29, 32, 98, 218, 725
LweI GCATC 8 cut(s) 527, 1066, 1096, 1450, 1463, 1485, 1570, 1660
MaeI CTAG 4 cut(s) 536, 875, 1170, 1677
MaeII ACGT 2 cut(s) 960, 1684
MaeIII GTNAC 3 cut(s) 1024, 1498, 1599
MalI GATC 9 cut(s) 32, 126, 264, 851, 910, 1049, 1134, 1400, 1632
MboI GATC 9 cut(s) 30, 124, 262, 849, 908, 1047, 1132, 1398, 1630
MboII GAAGA 8 cut(s) 60, 641, 707, 739, 1094, 1142, 1216, 1318
MflI RGATCY 1 cut(s) 1630
MhlI GDGCHC 3 cut(s) 546, 582, 1041
MlsI TGGCCA 1 cut(s) 666
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 3 cut(s) 890, 1271, 1626
MmeI TCCRAC 4 cut(s) 128, 255, 1215, 1269
Mox20I TGGCCA 1 cut(s) 666
Mph1103I ATGCAT 1 cut(s) 1478
MroXI GAANNNNTTC 1 cut(s) 1086
MscI TGGCCA 1 cut(s) 666
MseI TTAA 8 cut(s) 555, 732, 753, 992, 1070, 1256, 1548, 1574
MslI CAYNNNNRTG 1 cut(s) 523
Msp20I TGGCCA 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 1031
MspI CCGG 2 cut(s) 198, 1062
MspR9I CCNGG 2 cut(s) 109, 1063
Mva1269I GAATGC 1 cut(s) 909
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 3 cut(s) 1175, 1379, 1460
NciI CCSGG 1 cut(s) 1063
NdeII GATC 9 cut(s) 30, 124, 262, 849, 908, 1047, 1132, 1398, 1630
NlaIII CATG 3 cut(s) 31, 515, 528
NlaIV GGNNCC 4 cut(s) 173, 174, 507, 1585
NmuCI GTSAC 1 cut(s) 1599
NsbI TGCGCA 1 cut(s) 305
NsiI ATGCAT 1 cut(s) 1478
NspI RCATGY 2 cut(s) 515, 528
PaeI GCATGC 1 cut(s) 528
PciI ACATGT 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 1077
PctI GAATGC 1 cut(s) 909
PdmI GAANNNNTTC 1 cut(s) 1086
PfeI GAWTC 6 cut(s) 254, 614, 824, 845, 973, 1421
PkrI GCNGC 7 cut(s) 19, 22, 39, 80, 88, 208, 740
Ple19I CGATCG 1 cut(s) 1401
PleI GAGTC 3 cut(s) 889, 1270, 1625
PpsI GAGTC 3 cut(s) 889, 1270, 1625
PpuMI RGGWCCY 1 cut(s) 172
PscI ACATGT 1 cut(s) 511
PshAI GACNNNNGTC 1 cut(s) 177
PsiI TTATAA 1 cut(s) 1568
Psp124BI GAGCTC 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 4 cut(s) 173, 174, 507, 1585
PspPI GGNCC 3 cut(s) 172, 623, 832
PspPPI RGGWCCY 1 cut(s) 172
PstI CTGCAG 1 cut(s) 949
PstNI CAGNNNCTG 2 cut(s) 92, 953
PsuI RGATCY 1 cut(s) 1630
PvuI CGATCG 1 cut(s) 1401
PvuII CAGCTG 1 cut(s) 1031
RgaI GCGATCGC 1 cut(s) 1401
RsaI GTAC 5 cut(s) 115, 587, 963, 1585, 1591
RsaNI GTAC 5 cut(s) 114, 586, 962, 1584, 1590
RseI CAYNNNNRTG 1 cut(s) 523
SacI GAGCTC 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 1077
SaqAI TTAA 8 cut(s) 555, 732, 753, 992, 1070, 1256, 1548, 1574
SatI GCNGC 7 cut(s) 18, 21, 38, 79, 87, 207, 739
Sau3AI GATC 9 cut(s) 30, 124, 262, 849, 908, 1047, 1132, 1398, 1630
Sau96I GGNCC 3 cut(s) 172, 623, 832
SchI GAGTC 3 cut(s) 890, 1271, 1626
ScrFI CCNGG 2 cut(s) 109, 1063
SduI GDGCHC 3 cut(s) 546, 582, 1041
SfaAI GCGATCGC 1 cut(s) 1401
SfaNI GCATC 8 cut(s) 527, 1066, 1096, 1450, 1463, 1485, 1570, 1660
SfcI CTRYAG 2 cut(s) 547, 945
SgfI GCGATCGC 1 cut(s) 1401
SinI GGWCC 2 cut(s) 172, 623
SmiI ATTTAAAT 1 cut(s) 1257
SmiMI CAYNNNNRTG 1 cut(s) 523
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SphI GCATGC 1 cut(s) 528
SsiI CCGC 4 cut(s) 37, 78, 81, 1059
SspMI CTAG 4 cut(s) 536, 875, 1170, 1677
SstI GAGCTC 1 cut(s) 546
StyD4I CCNGG 2 cut(s) 107, 1061
StyI CCWWGG 3 cut(s) 661, 808, 874
SwaI ATTTAAAT 1 cut(s) 1257
TaaI ACNGT 7 cut(s) 94, 131, 238, 320, 796, 1021, 1281
TaiI ACGT 2 cut(s) 963, 1687
TaqI TCGA 3 cut(s) 848, 1449, 1662
TatI WGTACW 1 cut(s) 585
TauI GCSGC 2 cut(s) 40, 81
TfiI GAWTC 6 cut(s) 254, 614, 824, 845, 973, 1421
Tru1I TTAA 8 cut(s) 555, 732, 753, 992, 1070, 1256, 1548, 1574
Tru9I TTAA 8 cut(s) 555, 732, 753, 992, 1070, 1256, 1548, 1574
TscAI CASTG 3 cut(s) 97, 241, 1050
TseFI GTSAC 1 cut(s) 1599
TseI GCWGC 5 cut(s) 17, 20, 86, 206, 738
Tsp45I GTSAC 1 cut(s) 1599
TspDTI ATGAA 3 cut(s) 170, 267, 1656
TspGWI ACGGA 2 cut(s) 35, 700
TspRI CASTG 3 cut(s) 97, 241, 1050
VpaK11BI GGWCC 2 cut(s) 172, 623
XagI CCTNNNNNAGG 1 cut(s) 839
XapI RAATTY 2 cut(s) 357, 1006
XceI RCATGY 2 cut(s) 515, 528
XmaJI CCTAGG 1 cut(s) 874
XmnI GAANNNNTTC 1 cut(s) 1086
XspI CTAG 4 cut(s) 536, 875, 1170, 1677
Zsp2I ATGCAT 1 cut(s) 1478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.