Rroxscaffold_7G00196910

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002412
Physical Location & Seq
Reverse (-)
40411887 .. 40430290
18404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00196910.1

Sequence Viewer

Length: 1611 bp
ATGGCAGTGACGATCCACCAGCTGAGGAGGAAGAGAAGGAAGATGACGAACAGTTCAGTTGCAATTCCAGCTTTAATTCAATTCTGCAGTTCCAGCAATTCAGTTCTGCATTCCAGCTTTAATTCAATTCTGCAGTTCCAGCAATTCAGTTCTGCAGTTCCAGCTTCAATTCTGCAATTCTGCAGTTCAGTTCTGCAATTCCAGCTCGGCTATTTTCGACTTGGTGATGCAGTGATTAGGTCTAAAAGGTATGTTGAACCGGGATTAGAGGTGAGTAAACCTCACATGCCCAAAGTTCGGGGTTGGACCAGAAGGGGTCGACGGGAGTTTTTGCGAGAAAAGAAAGATGATGATTGTGCATATTTGTCATTGGGTATGCGTGAGTTTGAAATCGGTTGGAGTCAACGAAATCCGACCGTTGGATCTTCGTATAATTGCAAATCAGAGTTAAGGAAGCTGTCAAAAGCTTACCGGGAATTGATCTTGAAGTATTTGGAGCGAGTACGAAGCTTGGAGGAGTTAAGCCCAAAACAATATGAAGTAGTTGGGTCTGCCTTTCATAATCTGGGTCAGTTCTATATTGTACAGCGGAAGCTGGATGAAGCCCGTGTTTGCTATGAGCGTGCTTTGAAGATTAAACGCCGTGTTCTGGGTCTTAACCATACGAGTTATGCAGATACTATGTATCATCTTGGAACGGTGCTACACCTCCAAGGAAAAGAAAAGGACTCTGAGGCCCTTGTTCAAGATTTCATTCGAATACTTGAGGATGGTGGTCAAGGAGAATCGGTTATGTGCTTAAAGAGAATGCTATATCTTGCTCAGATATATCTGAAATCCAATCGATTTGAGGAGGCCGAGAAGGTACAAAGAAAGATCCTGCATATAATGGAAATAACAAAGGGATGGAATGCATTGGATACAGCAATAACAGCTGAAGGGCTCGCACTGATCCTCCAATCCACTGGGAGCTTAAAGGAAGCTGAAGAGCTTCTTGAAAGATGTCTTGATGCTCGAAAAACCTTACTTCCTCAAGATCACATTCAGATTGGTGTGAACTTGCTTCATATTGCTAGAATGGCAATGCTCAATTCCGACCAACTGAAGAATATTGACTATCCAAAAGCAAATTCTGAGCTTGATAAGGCCAAAGAAGTTTTACATGAGTCCATGAGGATAGCACAGTGTTCCTTGGATAAATTGATGAAGCAAAATAAGAAGATTCATAGTAATGGAGCACCAGGTGAATTGGGAAAGAATGGTCAGGTGCCATTAGTCATATTGTTGCAAGTTTCTGATGCCCTTGGCCTTTTGGAGATCACAAAGAAACAATTAAAAAGGTCAAAGCAGTCTGAGCGTGCACCTAACATCGAAGCTGAGAATGCACTATTTAAATGTATATCTGCTTACAGAGAGAATCGGAGCGAGAGAGCGAGAGATCGTGCGGATCGGAGTGCTGTTGTTGGATGTTGGATTAGGTGTGTGAAGTCGCGTATCGTCTGGCTGAAAGACGTTAAACTCAAGCGCTGCTTAGGAGGCAACCCAATTCAGAAGCAGGTACATCGAGGGGAAATAACCAACGTGGTAGCCGCTACAAGTCGAAGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

536

Amino Acids

61.2

Weight (kDa)

9.71

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_NPHP3 PF24885 159 - 298 4.5e-11 Nephrocystin-3 TPR domain
TPR_MalT PF17874 171 - 335 5.8e-09 MalT-like TPR region
TPR_12 PF13424 184 - 256 7e-13 Tetratricopeptide repeat
TPR_10 PF13374 184 - 221 3.6e-08 Tetratricopeptide repeat
TPR_12 PF13424 271 - 340 3e-09 Tetratricopeptide repeat
TPR_10 PF13374 308 - 348 1.7e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1546
AccB1I GGYRCC 1 cut(s) 1267
AccI GTMKAC 1 cut(s) 319
AccII CGCG 1 cut(s) 1492
AciI CCGC 3 cut(s) 589, 1445, 1590
AclWI GGATC 5 cut(s) 7, 430, 871, 946, 1455
AcsI RAATTY 1 cut(s) 1129
AcuI CTGAAG 3 cut(s) 957, 1005, 1124
AdeI CACNNNGTG 1 cut(s) 1244
AfaI GTAC 4 cut(s) 504, 585, 867, 1560
AfeI AGCGCT 1 cut(s) 1526
AfiI CCNNNNNNNGG 3 cut(s) 297, 419, 649
AgsI TTSAA 9 cut(s) 80, 126, 168, 257, 389, 487, 631, 746, 998
AjnI CCWGG 1 cut(s) 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 1114, 1146
Alw21I GWGCWC 2 cut(s) 1240, 1363
Alw44I GTGCAC 1 cut(s) 1359
AlwI GGATC 5 cut(s) 7, 430, 871, 946, 1455
Aor51HI AGCGCT 1 cut(s) 1526
AoxI GGCC 4 cut(s) 735, 855, 1146, 1306
ApaLI GTGCAC 1 cut(s) 1359
ApeKI GCWGC 1 cut(s) 1527
ApoI RAATTY 1 cut(s) 1129
Asp700I GAANNNNTTC 1 cut(s) 990
AspLEI GCGC 1 cut(s) 1527
AspS9I GGNCC 2 cut(s) 306, 736
AsuC2I CCSGG 2 cut(s) 261, 473
AsuHPI GGTGA 3 cut(s) 236, 283, 1256
AsuII TTCGAA 1 cut(s) 757
AvaII GGWCC 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 1363
BanI GGYRCC 1 cut(s) 1267
BanII GRGCYC 1 cut(s) 945
Bbv12I GWGCWC 2 cut(s) 1240, 1363
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 1514
BccI CCATC 2 cut(s) 764, 900
BceAI ACGGC 1 cut(s) 627
BcgI CGANNNNNNTGC 2 cut(s) 1544, 1578
BciT130I CCWGG 1 cut(s) 1242
BciVI GTATCC 1 cut(s) 913
BcnI CCSGG 2 cut(s) 261, 473
BfaI CTAG 1 cut(s) 1074
BfmI CTRYAG 4 cut(s) 85, 131, 153, 181
BfoI RGCGCY 1 cut(s) 1528
BfuAI ACCTGC 1 cut(s) 1546
BfuI GTATCC 1 cut(s) 913
BisI GCNGC 2 cut(s) 1528, 1590
BlsI GCNGC 2 cut(s) 1529, 1591
Bme1390I CCNGG 3 cut(s) 261, 473, 1242
Bme18I GGWCC 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 306, 736
BmiI GGNNCC 1 cut(s) 1269
BmrFI CCNGG 3 cut(s) 261, 473, 1242
BmrI ACTGGG 1 cut(s) 975
BmsI GCATC 3 cut(s) 217, 1000, 1288
BmuI ACTGGG 1 cut(s) 975
BplI GAGNNNNNCTC 2 cut(s) 265, 297
Bpu10I CCTNAGC 2 cut(s) 23, 1531
Bpu14I TTCGAA 1 cut(s) 757
BpuEI CTTGAG 3 cut(s) 785, 1017, 1505
BpuMI CCSGG 2 cut(s) 261, 473
Bsa29I ATCGAT 1 cut(s) 844
BsaJI CCNNGG 3 cut(s) 712, 1191, 1303
BsaXI ACNNNNNCTCC 4 cut(s) 774, 804, 939, 969
Bsc4I CCNNNNNNNGG 3 cut(s) 297, 419, 649
Bse1I ACTGG 1 cut(s) 970
Bse3DI GCAATG 1 cut(s) 1089
BseBI CCWGG 1 cut(s) 1242
BseCI ATCGAT 1 cut(s) 844
BseDI CCNNGG 3 cut(s) 712, 1191, 1303
BseGI GGATG 4 cut(s) 604, 775, 911, 1472
BseLI CCNNNNNNNGG 3 cut(s) 297, 419, 649
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 6 cut(s) 14, 723, 836, 1125, 1344, 1368
BseNI ACTGG 1 cut(s) 970
BseRI GAGGAG 3 cut(s) 40, 530, 866
BseSI GKGCMC 1 cut(s) 1363
BseXI GCAGC 1 cut(s) 1514
Bsh1236I CGCG 1 cut(s) 1492
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 4 cut(s) 737, 857, 1148, 1308
BshNI GGYRCC 1 cut(s) 1267
BshVI ATCGAT 1 cut(s) 844
BsiEI CGRYCG 1 cut(s) 417
BsiHKAI GWGCWC 2 cut(s) 1240, 1363
BsiSI CCGG 2 cut(s) 260, 472
BslI CCNNNNNNNGG 3 cut(s) 297, 419, 649
BsmI GAATGC 4 cut(s) 109, 813, 916, 1387
BsnI GGCC 4 cut(s) 737, 857, 1148, 1308
Bsp119I TTCGAA 1 cut(s) 757
Bsp1286I GDGCHC 3 cut(s) 945, 1240, 1363
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 9 cut(s) 12, 422, 480, 876, 951, 1036, 1317, 1438, 1447
BspACI CCGC 3 cut(s) 589, 1445, 1590
BspANI GGCC 4 cut(s) 737, 857, 1148, 1308
BspCNI CTCAG 6 cut(s) 15, 724, 835, 1126, 1345, 1369
BspDI ATCGAT 1 cut(s) 844
BspFNI CGCG 1 cut(s) 1492
BspLI GGNNCC 1 cut(s) 1269
BspMAI CTGCAG 4 cut(s) 89, 135, 157, 185
BspMI ACCTGC 1 cut(s) 1546
BspPI GGATC 5 cut(s) 7, 430, 871, 946, 1455
BspQI GCTCTTC 1 cut(s) 981
BspT104I TTCGAA 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 1267
BsrDI GCAATG 1 cut(s) 1089
BsrGI TGTACA 1 cut(s) 583
BsrI ACTGG 1 cut(s) 970
BssECI CCNNGG 3 cut(s) 712, 1191, 1303
BssMI GATC 9 cut(s) 12, 422, 480, 876, 951, 1036, 1317, 1438, 1447
BssT1I CCWWGG 3 cut(s) 712, 1191, 1303
Bst2UI CCWGG 1 cut(s) 1242
Bst4CI ACNGT 4 cut(s) 53, 418, 700, 1185
Bst6I CTCTTC 2 cut(s) 26, 981
BstAUI TGTACA 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 757
BstC8I GCNNGC 3 cut(s) 624, 945, 1359
BstDEI CTNAG 7 cut(s) 23, 732, 822, 1134, 1353, 1377, 1531
BstF5I GGATG 4 cut(s) 604, 775, 911, 1472
BstFNI CGCG 1 cut(s) 1492
BstH2I RGCGCY 1 cut(s) 1528
BstHHI GCGC 1 cut(s) 1527
BstKTI GATC 9 cut(s) 15, 425, 483, 879, 954, 1039, 1320, 1441, 1450
BstMBI GATC 9 cut(s) 12, 422, 480, 876, 951, 1036, 1317, 1438, 1447
BstMCI CGRYCG 1 cut(s) 417
BstNI CCWGG 1 cut(s) 1242
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 3 cut(s) 259, 471, 1240
BstSFI CTRYAG 4 cut(s) 85, 131, 153, 181
BstSLI GKGCMC 1 cut(s) 1363
BstUI CGCG 1 cut(s) 1492
BstV1I GCAGC 1 cut(s) 1514
BstX2I RGATCY 2 cut(s) 422, 876
BstXI CCANNNNNNTGG 1 cut(s) 965
BstYI RGATCY 2 cut(s) 422, 876
Bsu15I ATCGAT 1 cut(s) 844
BsuI GTATCC 1 cut(s) 913
BsuRI GGCC 4 cut(s) 737, 857, 1148, 1308
BsuTUI ATCGAT 1 cut(s) 844
BtsCI GGATG 4 cut(s) 604, 775, 911, 1472
BtsI GCAGTG 2 cut(s) 12, 237
BtsIMutI CAGTG 5 cut(s) 12, 237, 947, 963, 1190
BveI ACCTGC 1 cut(s) 1546
Cac8I GCNNGC 3 cut(s) 624, 945, 1359
CfoI GCGC 1 cut(s) 1527
Cfr13I GGNCC 2 cut(s) 306, 736
ClaI ATCGAT 1 cut(s) 844
CsiI ACCWGGT 1 cut(s) 1240
Csp6I GTAC 4 cut(s) 503, 584, 866, 1559
CviAII CATG 3 cut(s) 286, 1163, 1171
CviQI GTAC 4 cut(s) 503, 584, 866, 1559
DdeI CTNAG 7 cut(s) 23, 732, 822, 1134, 1353, 1377, 1531
DpnI GATC 9 cut(s) 14, 424, 482, 878, 953, 1038, 1319, 1440, 1449
DpnII GATC 9 cut(s) 12, 422, 480, 876, 951, 1036, 1317, 1438, 1447
DraI TTTAAA 1 cut(s) 1393
DraIII CACNNNGTG 1 cut(s) 1244
Eam1104I CTCTTC 2 cut(s) 26, 981
EarI CTCTTC 2 cut(s) 26, 981
Eco130I CCWWGG 3 cut(s) 712, 1191, 1303
Eco24I GRGCYC 1 cut(s) 945
Eco47I GGWCC 1 cut(s) 306
Eco47III AGCGCT 1 cut(s) 1526
Eco57I CTGAAG 3 cut(s) 957, 1005, 1124
EcoO109I RGGNCCY 1 cut(s) 736
EcoRII CCWGG 1 cut(s) 1240
EcoT14I CCWWGG 3 cut(s) 712, 1191, 1303
EcoT22I ATGCAT 1 cut(s) 916
EcoT38I GRGCYC 1 cut(s) 945
ErhI CCWWGG 3 cut(s) 712, 1191, 1303
FaeI CATG 3 cut(s) 289, 1166, 1174
FalI AAGNNNNNCTT 4 cut(s) 978, 1010, 1514, 1546
FatI CATG 3 cut(s) 285, 1162, 1170
FblI GTMKAC 1 cut(s) 319
Fnu4HI GCNGC 2 cut(s) 1528, 1590
FokI GGATG 4 cut(s) 611, 782, 918, 1479
FriOI GRGCYC 1 cut(s) 945
Fsp4HI GCNGC 2 cut(s) 1528, 1590
FspBI CTAG 1 cut(s) 1074
GlaI GCGC 1 cut(s) 1526
GluI GCNGC 2 cut(s) 1528, 1590
HaeII RGCGCY 1 cut(s) 1528
HaeIII GGCC 4 cut(s) 737, 857, 1148, 1308
HapII CCGG 2 cut(s) 260, 472
HhaI GCGC 1 cut(s) 1527
Hin1II CATG 3 cut(s) 289, 1166, 1174
Hin6I GCGC 1 cut(s) 1525
HinP1I GCGC 1 cut(s) 1525
HincII GTYRAC 2 cut(s) 320, 404
HindII GTYRAC 2 cut(s) 320, 404
HindIII AAGCTT 2 cut(s) 465, 508
HinfI GANTC 6 cut(s) 400, 728, 785, 1166, 1222, 1417
HpaII CCGG 2 cut(s) 260, 472
HphI GGTGA 3 cut(s) 236, 283, 1256
Hpy166II GTNNAC 5 cut(s) 278, 320, 404, 1057, 1361
Hpy188III TCNNGA 5 cut(s) 484, 746, 995, 1007, 1034
Hpy8I GTNNAC 5 cut(s) 278, 320, 404, 1057, 1361
Hpy99I CGWCG 1 cut(s) 324
HpyAV CCTTC 4 cut(s) 30, 306, 856, 932
HpyCH4III ACNGT 4 cut(s) 53, 418, 700, 1185
HpyCH4IV ACGT 2 cut(s) 1512, 1581
HpyF3I CTNAG 7 cut(s) 23, 732, 822, 1134, 1353, 1377, 1531
HpySE526I ACGT 2 cut(s) 1512, 1581
Hsp92II CATG 3 cut(s) 289, 1166, 1174
HspAI GCGC 1 cut(s) 1525
Kzo9I GATC 9 cut(s) 12, 422, 480, 876, 951, 1036, 1317, 1438, 1447
LguI GCTCTTC 1 cut(s) 981
LmnI GCTCC 4 cut(s) 496, 969, 1235, 1422
Lsp1109I GCAGC 1 cut(s) 1514
LweI GCATC 3 cut(s) 217, 1000, 1288
MabI ACCWGGT 1 cut(s) 1240
MaeI CTAG 1 cut(s) 1074
MaeII ACGT 2 cut(s) 1512, 1581
MaeIII GTNAC 1 cut(s) 7
MalI GATC 9 cut(s) 14, 424, 482, 878, 953, 1038, 1319, 1440, 1449
MboI GATC 9 cut(s) 12, 422, 480, 876, 951, 1036, 1317, 1438, 1447
MboII GAAGA 7 cut(s) 43, 52, 417, 643, 998, 1117, 1231
MflI RGATCY 2 cut(s) 422, 876
MhlI GDGCHC 3 cut(s) 945, 1240, 1363
MlyI GAGTC 3 cut(s) 409, 722, 1175
MmeI TCCRAC 7 cut(s) 284, 377, 400, 437, 1119, 1444, 1451
Mph1103I ATGCAT 1 cut(s) 916
MroXI GAANNNNTTC 1 cut(s) 990
MslI CAYNNNNRTG 1 cut(s) 1230
MspA1I CMGCKG 3 cut(s) 22, 589, 935
MspI CCGG 2 cut(s) 260, 472
MspR9I CCNGG 3 cut(s) 261, 473, 1242
Mva1269I GAATGC 4 cut(s) 109, 813, 916, 1387
MvaI CCWGG 1 cut(s) 1242
MvnI CGCG 1 cut(s) 1492
NciI CCSGG 2 cut(s) 261, 473
NdeII GATC 9 cut(s) 12, 422, 480, 876, 951, 1036, 1317, 1438, 1447
NlaIII CATG 3 cut(s) 289, 1166, 1174
NlaIV GGNNCC 1 cut(s) 1269
NmeAIII GCCGAG 2 cut(s) 186, 883
NmuCI GTSAC 1 cut(s) 7
NsiI ATGCAT 1 cut(s) 916
NspI RCATGY 1 cut(s) 289
NspV TTCGAA 1 cut(s) 757
PciSI GCTCTTC 1 cut(s) 981
PctI GAATGC 4 cut(s) 109, 813, 916, 1387
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 3 cut(s) 785, 1222, 1417
PkrI GCNGC 2 cut(s) 1529, 1591
PleI GAGTC 3 cut(s) 408, 722, 1174
PpsI GAGTC 3 cut(s) 408, 722, 1174
Psp6I CCWGG 1 cut(s) 1240
PspGI CCWGG 1 cut(s) 1240
PspN4I GGNNCC 1 cut(s) 1269
PspPI GGNCC 2 cut(s) 306, 736
PstI CTGCAG 4 cut(s) 89, 135, 157, 185
PsuI RGATCY 2 cut(s) 422, 876
PvuII CAGCTG 2 cut(s) 22, 935
RsaI GTAC 4 cut(s) 504, 585, 867, 1560
RsaNI GTAC 4 cut(s) 503, 584, 866, 1559
RseI CAYNNNNRTG 1 cut(s) 1230
SalI GTCGAC 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 981
SatI GCNGC 2 cut(s) 1528, 1590
Sau3AI GATC 9 cut(s) 12, 422, 480, 876, 951, 1036, 1317, 1438, 1447
Sau96I GGNCC 2 cut(s) 306, 736
SchI GAGTC 3 cut(s) 409, 722, 1175
ScrFI CCNGG 3 cut(s) 261, 473, 1242
SduI GDGCHC 3 cut(s) 945, 1240, 1363
SexAI ACCWGGT 1 cut(s) 1240
SfaNI GCATC 3 cut(s) 217, 1000, 1288
SfcI CTRYAG 4 cut(s) 85, 131, 153, 181
SfuI TTCGAA 1 cut(s) 757
SinI GGWCC 1 cut(s) 306
SmiI ATTTAAAT 1 cut(s) 1393
SmiMI CAYNNNNRTG 1 cut(s) 1230
SmlI CTYRAG 3 cut(s) 764, 1032, 1520
SmoI CTYRAG 3 cut(s) 764, 1032, 1520
SsiI CCGC 3 cut(s) 589, 1445, 1590
SspI AATATT 1 cut(s) 1111
SspMI CTAG 1 cut(s) 1074
StyD4I CCNGG 3 cut(s) 259, 471, 1240
StyI CCWWGG 3 cut(s) 712, 1191, 1303
SwaI ATTTAAAT 1 cut(s) 1393
TaaI ACNGT 4 cut(s) 53, 418, 700, 1185
TaiI ACGT 2 cut(s) 1515, 1584
TaqI TCGA 8 cut(s) 217, 319, 757, 844, 1015, 1371, 1564, 1600
TatI WGTACW 1 cut(s) 583
TauI GCSGC 1 cut(s) 1592
TfiI GAWTC 3 cut(s) 785, 1222, 1417
TscAI CASTG 5 cut(s) 12, 237, 954, 970, 1190
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 1 cut(s) 1527
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 7 cut(s) 548, 552, 615, 742, 1055, 1214, 1220
TspRI CASTG 5 cut(s) 12, 237, 954, 970, 1190
VneI GTGCAC 1 cut(s) 1359
VpaK11BI GGWCC 1 cut(s) 306
XapI RAATTY 1 cut(s) 1129
XceI RCATGY 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 319
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 1 cut(s) 1074
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.