RchiOBHm_Chr3g0491391

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002412
Physical Location & Seq
Reverse (-)
38133691 .. 38146935
13245 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45438

Sequence Viewer

Length: 1530 bp
ATGGACCCTCGGCTCTGGTTTTTTCTATCTGGAAATGCAGCAATTATTCTTGCCATAAAGTCCAACACCGTCTTGGCAAAAGATGCATCACTTGATAATGAGGCACAGGGGATGGAAGGGGATGACACAATGCAATTACGCAGAGTTGAGGATGCTTCTGTCAAATCTAACATACATACCTCTAAGTGGAGAGTTTTTACAGATAAAGGAAGGGAATTCTTCCTTCAGGCAAGCATCCTATGCCTATGTGGAAAAGTGGAGGAAGCTGAAAAGCTCTTTCTCGCAGCTTTACAGGAAGCTGAAGAAGGTTTTGGGAAGAGGGATCCGCATGTTGCATCTGCAAGCAATAATTTAGCAGAGCTCTATAGAGTTAATAAGCTATATCATAAGGCAGAGCCATTGTATTTGGAGGCCATCAGCATACTGGAGGAGTCATTTGGTCCTGAAGATATACGAGTTGGGTCTGCCTTTCATAACCTGGGTCAGTTCTATATTGTACAGCGGAAGCTGGATGAAGCCCGTGTTTGCTATGAGCGTGCTTTGAAGATTAAACGCCTTGTTCTGGGTCTTAACCATACAGGTTATGCAGATATGATGTATCATCTTGGAACGGTGCTACACCTCCAAGGAAAAGAAAAGGACTCTGAGGCCCTCGTTCAAGATTCCATTCGAATACTTGAGGATGGTGGTCAAGGAGAGTCGGTTATGTGCTTAAAGAGAATGCGATATCTTGCTCAGATATATCTGAAATCCAATCGATTTGAGGAGGCCGAGAAGGTACAAAGAAAGATCCTGCATATAATGGAAATAACAAAGGGATGGAATGCATTGGATACAGCAATAACAGCTGAAGGGCTCGCACTGATCCTCCAATCCACTGGGAGCTTAAAGGAAGCTGAAGAGCTTCTTGAAAGATGTCTTGATGCTCGAAAAGCCTTACTTCCTCAAGATCACGTTCAGATTGGTGTGAACTTGCTTCATATTGCTAGAGTGGCAATGCTCAATTCCGACCAACTGAAGAATATTGACTATCCAAAAGCAATTTCTGAGCTTGATAAGGCCAAAGAAGTTTTACATGAGTCCATGAGGATAGCACAATGTTCCTTGGATAAATTGATGAAGCAAAATAAGAAGATTCGTAGTAATGGAGCACCAGGTGAAATTGGAAAGAATGGTCAGGTGCCATTAGTCATATTGTTGCAAGCTTCTGATGCTCTTGGCCTTTTGGAGATCACAAAGAAACAATTACAAGGGTCAAAGCAGTCTGAGCATGCACCTAATATTGAAGCTGAGAATGCACTATTTAAATGTATATCTGCTTACAAAGAGTTCGTAACTGAGAGATCAATCCCTGAGTCTCCTGAAACAAAGGCCGAGTATCTTAAATGTTTGAAGCATCTTTTGAGCTTGAATAGTGATAGCACTACAGAAAGAACACAGCACTCTACTGGAGTCAATTTGCAAGATCTGAGAGACGAAATTAAGCGTCTTGAAGTTGAACTTTCTCCTCATAGGAAACGCAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

509

Amino Acids

57.34

Weight (kDa)

6.6

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 77 - 144 1.1e-09 Tetratricopeptide repeat
TPR_NPHP3 PF24885 87 - 185 2.4e-11 Nephrocystin-3 TPR domain
TPR_12 PF13424 111 - 162 1.3e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 153 - 269 4.4e-08 Nephrocystin-3 TPR domain
TPR_12 PF13424 154 - 227 6.9e-13 Tetratricopeptide repeat
TPR_10 PF13374 155 - 192 1.6e-06 Tetratricopeptide repeat
TPR_MalT PF17874 200 - 328 3.8e-06 MalT-like TPR region
TPR_12 PF13424 243 - 311 3.3e-09 Tetratricopeptide repeat
TPR_10 PF13374 279 - 318 1.4e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1180
AciI CCGC 2 cut(s) 326, 502
AclWI GGATC 4 cut(s) 317, 330, 784, 859
AcsI RAATTY 2 cut(s) 215, 1524
AcuI CTGAAG 6 cut(s) 209, 321, 465, 870, 918, 1037
AdeI CACNNNGTG 1 cut(s) 1157
AfaI GTAC 2 cut(s) 498, 780
AfiI CCNNNNNNNGG 2 cut(s) 186, 562
AgsI TTSAA 8 cut(s) 544, 659, 911, 1286, 1393, 1411, 1493, 1499
AjnI CCWGG 2 cut(s) 477, 1153
AjuI GAANNNNNNNTTGG 4 cut(s) 294, 326, 1027, 1059
Alw21I GWGCWC 2 cut(s) 363, 1153
Alw26I GTCTC 2 cut(s) 1362, 1467
AlwI GGATC 4 cut(s) 317, 330, 784, 859
AoxI GGCC 6 cut(s) 411, 648, 768, 1059, 1219, 1371
ApeKI GCWGC 2 cut(s) 38, 284
ApoI RAATTY 2 cut(s) 215, 1524
Asp700I GAANNNNTTC 1 cut(s) 903
AspS9I GGNCC 3 cut(s) 4, 440, 649
AsuHPI GGTGA 1 cut(s) 1169
AsuII TTCGAA 1 cut(s) 670
AvaII GGWCC 2 cut(s) 4, 440
BamHI GGATCC 1 cut(s) 322
BanI GGYRCC 1 cut(s) 1180
BanII GRGCYC 2 cut(s) 363, 858
Bbv12I GWGCWC 2 cut(s) 363, 1153
BbvI GCAGC 2 cut(s) 50, 296
BccI CCATC 4 cut(s) 106, 422, 677, 813
BciT130I CCWGG 2 cut(s) 479, 1155
BciVI GTATCC 1 cut(s) 826
BcoDI GTCTC 2 cut(s) 1362, 1467
BfaI CTAG 1 cut(s) 987
BfmI CTRYAG 2 cut(s) 364, 1425
BfuI GTATCC 1 cut(s) 826
BglII AGATCT 1 cut(s) 1465
BisI GCNGC 2 cut(s) 39, 285
BlsI GCNGC 2 cut(s) 40, 286
Bme1390I CCNGG 2 cut(s) 479, 1155
Bme18I GGWCC 2 cut(s) 4, 440
BmgT120I GGNCC 3 cut(s) 4, 440, 649
BmiI GGNNCC 3 cut(s) 6, 324, 1182
BmrFI CCNGG 2 cut(s) 479, 1155
BmrI ACTGGG 1 cut(s) 888
BmsI GCATC 8 cut(s) 73, 95, 142, 243, 344, 913, 1201, 1405
BmuI ACTGGG 1 cut(s) 888
BpmI CTGGAG 2 cut(s) 446, 1470
Bpu14I TTCGAA 1 cut(s) 670
BpuEI CTTGAG 2 cut(s) 698, 930
Bsa29I ATCGAT 1 cut(s) 757
BsaJI CCNNGG 4 cut(s) 8, 478, 625, 1104
BsaXI ACNNNNNCTCC 4 cut(s) 687, 717, 852, 882
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 562
Bse1I ACTGG 3 cut(s) 429, 883, 1453
Bse3DI GCAATG 1 cut(s) 1002
BseBI CCWGG 2 cut(s) 479, 1155
BseCI ATCGAT 1 cut(s) 757
BseDI CCNNGG 4 cut(s) 8, 478, 625, 1104
BseGI GGATG 7 cut(s) 117, 127, 157, 234, 517, 688, 824
BseLI CCNNNNNNNGG 2 cut(s) 186, 562
BseMI GCAATG 1 cut(s) 1002
BseMII CTCAG 8 cut(s) 636, 749, 1038, 1257, 1281, 1329, 1344, 1460
BseNI ACTGG 3 cut(s) 429, 883, 1453
BseRI GAGGAG 3 cut(s) 443, 779, 1497
BseXI GCAGC 2 cut(s) 50, 296
BshFI GGCC 6 cut(s) 413, 650, 770, 1061, 1221, 1373
BshNI GGYRCC 1 cut(s) 1180
BshVI ATCGAT 1 cut(s) 757
BsiHKAI GWGCWC 2 cut(s) 363, 1153
BslI CCNNNNNNNGG 2 cut(s) 186, 562
BsmAI GTCTC 2 cut(s) 1362, 1467
BsmBI CGTCTC 1 cut(s) 1467
BsmI GAATGC 3 cut(s) 726, 829, 1300
BsnI GGCC 6 cut(s) 413, 650, 770, 1061, 1221, 1373
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 363, 858, 1153
Bsp1407I TGTACA 1 cut(s) 496
Bsp143I GATC 7 cut(s) 322, 789, 864, 949, 1230, 1343, 1465
BspACI CCGC 2 cut(s) 326, 502
BspANI GGCC 6 cut(s) 413, 650, 770, 1061, 1221, 1373
BspCNI CTCAG 8 cut(s) 637, 748, 1039, 1258, 1282, 1330, 1345, 1461
BspDI ATCGAT 1 cut(s) 757
BspLI GGNNCC 3 cut(s) 6, 324, 1182
BspPI GGATC 4 cut(s) 317, 330, 784, 859
BspQI GCTCTTC 1 cut(s) 894
BspT104I TTCGAA 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 1180
BsrDI GCAATG 1 cut(s) 1002
BsrGI TGTACA 1 cut(s) 496
BsrI ACTGG 3 cut(s) 429, 883, 1453
BssECI CCNNGG 4 cut(s) 8, 478, 625, 1104
BssMI GATC 7 cut(s) 322, 789, 864, 949, 1230, 1343, 1465
BssT1I CCWWGG 2 cut(s) 625, 1104
Bst2UI CCWGG 2 cut(s) 479, 1155
Bst4CI ACNGT 2 cut(s) 70, 613
Bst6I CTCTTC 2 cut(s) 311, 894
BstAPI GCANNNNNTGC 2 cut(s) 83, 240
BstAUI TGTACA 1 cut(s) 496
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 6 cut(s) 232, 343, 537, 858, 1203, 1272
BstDEI CTNAG 9 cut(s) 183, 645, 735, 1047, 1266, 1290, 1338, 1353, 1469
BstF5I GGATG 7 cut(s) 117, 127, 157, 234, 517, 688, 824
BstKTI GATC 7 cut(s) 325, 792, 867, 952, 1233, 1346, 1468
BstMAI GTCTC 2 cut(s) 1362, 1467
BstMBI GATC 7 cut(s) 322, 789, 864, 949, 1230, 1343, 1465
BstMWI GCNNNNNNNGC 8 cut(s) 83, 240, 845, 932, 992, 1211, 1267, 1295
BstNI CCWGG 2 cut(s) 479, 1155
BstNSI RCATGY 2 cut(s) 332, 1274
BstSCI CCNGG 2 cut(s) 477, 1153
BstSFI CTRYAG 2 cut(s) 364, 1425
BstV1I GCAGC 2 cut(s) 50, 296
BstX2I RGATCY 3 cut(s) 322, 789, 1465
BstXI CCANNNNNNTGG 1 cut(s) 878
BstYI RGATCY 3 cut(s) 322, 789, 1465
Bsu15I ATCGAT 1 cut(s) 757
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 6 cut(s) 413, 650, 770, 1061, 1221, 1373
BsuTUI ATCGAT 1 cut(s) 757
BtsCI GGATG 7 cut(s) 117, 127, 157, 234, 517, 688, 824
BtsIMutI CAGTG 2 cut(s) 860, 876
Cac8I GCNNGC 6 cut(s) 232, 343, 537, 858, 1203, 1272
Cfr13I GGNCC 3 cut(s) 4, 440, 649
ClaI ATCGAT 1 cut(s) 757
CseI GACGC 1 cut(s) 1475
CsiI ACCWGGT 1 cut(s) 1153
Csp6I GTAC 2 cut(s) 497, 779
CviAII CATG 4 cut(s) 329, 1076, 1084, 1271
CviQI GTAC 2 cut(s) 497, 779
DdeI CTNAG 9 cut(s) 183, 645, 735, 1047, 1266, 1290, 1338, 1353, 1469
DpnI GATC 7 cut(s) 324, 791, 866, 951, 1232, 1345, 1467
DpnII GATC 7 cut(s) 322, 789, 864, 949, 1230, 1343, 1465
DraI TTTAAA 1 cut(s) 1306
DraIII CACNNNGTG 1 cut(s) 1157
Eam1104I CTCTTC 2 cut(s) 311, 894
EarI CTCTTC 2 cut(s) 311, 894
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 2 cut(s) 625, 1104
Eco24I GRGCYC 2 cut(s) 363, 858
Eco32I GATATC 1 cut(s) 728
Eco47I GGWCC 2 cut(s) 4, 440
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 6 cut(s) 209, 321, 465, 870, 918, 1037
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 649
EcoRI GAATTC 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 477, 1153
EcoRV GATATC 1 cut(s) 728
EcoT14I CCWWGG 2 cut(s) 625, 1104
EcoT22I ATGCAT 2 cut(s) 88, 829
EcoT38I GRGCYC 2 cut(s) 363, 858
ErhI CCWWGG 2 cut(s) 625, 1104
Esp3I CGTCTC 1 cut(s) 1467
FaeI CATG 4 cut(s) 332, 1079, 1087, 1274
FalI AAGNNNNNCTT 6 cut(s) 891, 923, 924, 956, 1485, 1517
FatI CATG 4 cut(s) 328, 1075, 1083, 1270
Fnu4HI GCNGC 2 cut(s) 39, 285
FokI GGATG 7 cut(s) 124, 134, 164, 221, 524, 695, 831
FriOI GRGCYC 2 cut(s) 363, 858
Fsp4HI GCNGC 2 cut(s) 39, 285
FspBI CTAG 1 cut(s) 987
GluI GCNGC 2 cut(s) 39, 285
GsuI CTGGAG 2 cut(s) 446, 1470
HaeIII GGCC 6 cut(s) 413, 650, 770, 1061, 1221, 1373
HgaI GACGC 1 cut(s) 1475
Hin1II CATG 4 cut(s) 332, 1079, 1087, 1274
HindIII AAGCTT 1 cut(s) 1203
HinfI GANTC 8 cut(s) 431, 641, 662, 698, 1079, 1135, 1355, 1452
HphI GGTGA 1 cut(s) 1169
Hpy166II GTNNAC 1 cut(s) 970
Hpy188I TCNGA 9 cut(s) 646, 738, 747, 960, 1009, 1048, 1210, 1267, 1470
Hpy188III TCNNGA 8 cut(s) 30, 443, 659, 908, 920, 947, 1361, 1490
Hpy8I GTNNAC 1 cut(s) 970
HpyAV CCTTC 6 cut(s) 110, 204, 233, 299, 769, 845
HpyCH4III ACNGT 2 cut(s) 70, 613
HpyCH4IV ACGT 1 cut(s) 954
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 240, 845, 932, 992, 1211, 1267, 1295
HpyF3I CTNAG 9 cut(s) 183, 645, 735, 1047, 1266, 1290, 1338, 1353, 1469
HpySE526I ACGT 1 cut(s) 954
Hsp92II CATG 4 cut(s) 332, 1079, 1087, 1274
Kzo9I GATC 7 cut(s) 322, 789, 864, 949, 1230, 1343, 1465
LguI GCTCTTC 1 cut(s) 894
LmnI GCTCC 2 cut(s) 882, 1148
Lsp1109I GCAGC 2 cut(s) 50, 296
LweI GCATC 8 cut(s) 73, 95, 142, 243, 344, 913, 1201, 1405
MabI ACCWGGT 1 cut(s) 1153
MaeI CTAG 1 cut(s) 987
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 1 cut(s) 1333
MalI GATC 7 cut(s) 324, 791, 866, 951, 1232, 1345, 1467
MboI GATC 7 cut(s) 322, 789, 864, 949, 1230, 1343, 1465
MboII GAAGA 8 cut(s) 211, 314, 328, 458, 556, 911, 1030, 1144
MflI RGATCY 3 cut(s) 322, 789, 1465
MhlI GDGCHC 3 cut(s) 363, 858, 1153
MlyI GAGTC 6 cut(s) 440, 635, 707, 1088, 1364, 1461
MmeI TCCRAC 2 cut(s) 87, 1032
Mph1103I ATGCAT 2 cut(s) 88, 829
MroXI GAANNNNTTC 1 cut(s) 903
MseI TTAA 8 cut(s) 372, 549, 570, 713, 887, 1305, 1383, 1482
MspA1I CMGCKG 2 cut(s) 502, 848
MspR9I CCNGG 2 cut(s) 479, 1155
Mva1269I GAATGC 3 cut(s) 726, 829, 1300
MvaI CCWGG 2 cut(s) 479, 1155
MwoI GCNNNNNNNGC 8 cut(s) 83, 240, 845, 932, 992, 1211, 1267, 1295
NdeII GATC 7 cut(s) 322, 789, 864, 949, 1230, 1343, 1465
NlaIII CATG 4 cut(s) 332, 1079, 1087, 1274
NlaIV GGNNCC 3 cut(s) 6, 324, 1182
NmeAIII GCCGAG 2 cut(s) 796, 1399
NsiI ATGCAT 2 cut(s) 88, 829
NspI RCATGY 2 cut(s) 332, 1274
NspV TTCGAA 1 cut(s) 670
PaeI GCATGC 1 cut(s) 1274
PciSI GCTCTTC 1 cut(s) 894
PctI GAATGC 3 cut(s) 726, 829, 1300
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 2 cut(s) 662, 1135
PkrI GCNGC 2 cut(s) 40, 286
PleI GAGTC 6 cut(s) 439, 635, 706, 1087, 1363, 1460
PpsI GAGTC 6 cut(s) 439, 635, 706, 1087, 1363, 1460
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 477, 1153
PspGI CCWGG 2 cut(s) 477, 1153
PspN4I GGNNCC 3 cut(s) 6, 324, 1182
PspPI GGNCC 3 cut(s) 4, 440, 649
PsuI RGATCY 3 cut(s) 322, 789, 1465
PvuII CAGCTG 1 cut(s) 848
RsaI GTAC 2 cut(s) 498, 780
RsaNI GTAC 2 cut(s) 497, 779
SacI GAGCTC 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 8 cut(s) 372, 549, 570, 713, 887, 1305, 1383, 1482
SatI GCNGC 2 cut(s) 39, 285
Sau3AI GATC 7 cut(s) 322, 789, 864, 949, 1230, 1343, 1465
Sau96I GGNCC 3 cut(s) 4, 440, 649
SchI GAGTC 6 cut(s) 440, 635, 707, 1088, 1364, 1461
ScrFI CCNGG 2 cut(s) 479, 1155
SduI GDGCHC 3 cut(s) 363, 858, 1153
SexAI ACCWGGT 1 cut(s) 1153
SfaNI GCATC 8 cut(s) 73, 95, 142, 243, 344, 913, 1201, 1405
SfcI CTRYAG 2 cut(s) 364, 1425
SfuI TTCGAA 1 cut(s) 670
SinI GGWCC 2 cut(s) 4, 440
SmiI ATTTAAAT 1 cut(s) 1306
SmlI CTYRAG 2 cut(s) 677, 945
SmoI CTYRAG 2 cut(s) 677, 945
SphI GCATGC 1 cut(s) 1274
SsiI CCGC 2 cut(s) 326, 502
SspI AATATT 2 cut(s) 1024, 1282
SspMI CTAG 1 cut(s) 987
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 477, 1153
StyI CCWWGG 2 cut(s) 625, 1104
SwaI ATTTAAAT 1 cut(s) 1306
TaaI ACNGT 2 cut(s) 70, 613
TaiI ACGT 1 cut(s) 957
TaqI TCGA 3 cut(s) 670, 757, 928
TatI WGTACW 1 cut(s) 496
TfiI GAWTC 2 cut(s) 662, 1135
Tru1I TTAA 8 cut(s) 372, 549, 570, 713, 887, 1305, 1383, 1482
Tru9I TTAA 8 cut(s) 372, 549, 570, 713, 887, 1305, 1383, 1482
TscAI CASTG 2 cut(s) 867, 883
TseI GCWGC 2 cut(s) 38, 284
TspDTI ATGAA 4 cut(s) 461, 528, 968, 1133
TspRI CASTG 2 cut(s) 867, 883
VpaK11BI GGWCC 2 cut(s) 4, 440
XapI RAATTY 2 cut(s) 215, 1524
XceI RCATGY 2 cut(s) 332, 1274
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 421
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 1 cut(s) 987
Zsp2I ATGCAT 2 cut(s) 88, 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.