Rmu_sc0006034.1_g000001

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006034.1
OG0002412
Physical Location & Seq
Reverse (-)
1 .. 8963
8963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006034.1_g000001.1.cds

Sequence Viewer

Length: 885 bp
atgtcttgtgtgcggcgagcggtagctaaggtggtgaaggagaagacgcatatagttaaccgttttctctctccttcttcttcaactgcgggaaccggaaccatgactactgctccgttcttctccggcggtactggcggttacaagttttctgatcagcattggaggatggaccctcggctctggttttttctatctggaaatgcagcaattattcttgccataaattccaacaccgtcttggcaaaagatgcatcacttgataatgaggcacaggggatggaaggggatgacacaatgcaattacgcagagttgaggatgcttctgtcaaatctaacatacatacctctaagtggagagtttttacagataaagggagggaattcttccttcaggcaagcatcctatgcctatgtggaaaagtggaggaagctgaaaagctctttctcgcagctttacaggaagctgaagaaggttttgggaagagggatccgcatgttgcatctgcaagcaataatttaggatggaatgcattggatacagcaataacagctgaagggctcgcactgatcctccaatccactgggagcttaaaggaagctgaagagcttcttgaaagatgtcttgatgctcgaaaagccttacttcctcaagatcacgttcagattggtgtgaacttgcttcatattgctagagtggcaatgctcaattccgaccaactgaagaatattgactatccaaaagcaatttctgagcttgataaggccaaagaagttttacatgagtccatgaggatagcacaatgttccttggataaattgatgaagcaaaataagaagattcgtagtaatggagcaccaggtgaaattggaaagaatggtcag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

295

Amino Acids

32.37

Weight (kDa)

7.67

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 20
AciI CCGC 6 cut(s) 13, 20, 89, 129, 138, 494
AclWI GGATC 3 cut(s) 485, 498, 565
AcsI RAATTY 2 cut(s) 226, 383
AcuI CTGAAG 5 cut(s) 377, 489, 576, 624, 743
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 1 cut(s) 354
AgsI TTSAA 2 cut(s) 84, 617
AjnI CCWGG 1 cut(s) 859
AjuI GAANNNNNNNTTGG 4 cut(s) 462, 494, 733, 765
Alw21I GWGCWC 1 cut(s) 859
AlwI GGATC 3 cut(s) 485, 498, 565
AoxI GGCC 1 cut(s) 765
ApeKI GCWGC 2 cut(s) 206, 452
ApoI RAATTY 2 cut(s) 226, 383
Asp700I GAANNNNTTC 1 cut(s) 609
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 46, 875
AvaII GGWCC 1 cut(s) 172
BamHI GGATCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 564
BbsI GAAGAC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 859
BbvI GCAGC 2 cut(s) 218, 464
BccI CCATC 3 cut(s) 163, 274, 519
BciT130I CCWGG 1 cut(s) 861
BciVI GTATCC 1 cut(s) 532
BclI TGATCA 1 cut(s) 154
BfaI CTAG 1 cut(s) 693
BfuI GTATCC 1 cut(s) 532
BisI GCNGC 3 cut(s) 14, 207, 453
BlsI GCNGC 3 cut(s) 15, 208, 454
Bme1390I CCNGG 1 cut(s) 861
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 4 cut(s) 94, 100, 174, 492
BmrFI CCNGG 1 cut(s) 861
BmrI ACTGGG 1 cut(s) 594
BmsI GCATC 6 cut(s) 241, 263, 310, 411, 512, 619
BmuI ACTGGG 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 636
BsaJI CCNNGG 2 cut(s) 176, 810
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 558, 588
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse1I ACTGG 2 cut(s) 139, 589
Bse3DI GCAATG 1 cut(s) 708
BseBI CCWGG 1 cut(s) 861
BseDI CCNNGG 2 cut(s) 176, 810
BseGI GGATG 6 cut(s) 174, 285, 295, 325, 402, 530
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 1 cut(s) 744
BseNI ACTGG 2 cut(s) 139, 589
BseXI GCAGC 2 cut(s) 218, 464
BshFI GGCC 1 cut(s) 767
BsiHKAI GWGCWC 1 cut(s) 859
BsiSI CCGG 2 cut(s) 96, 126
BslI CCNNNNNNNGG 1 cut(s) 354
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 564, 859
Bsp143I GATC 4 cut(s) 154, 490, 570, 655
BspACI CCGC 6 cut(s) 13, 20, 89, 129, 138, 494
BspANI GGCC 1 cut(s) 767
BspCNI CTCAG 1 cut(s) 745
BspLI GGNNCC 4 cut(s) 94, 100, 174, 492
BspPI GGATC 3 cut(s) 485, 498, 565
BspQI GCTCTTC 1 cut(s) 600
BsrBI CCGCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 708
BsrI ACTGG 2 cut(s) 139, 589
BssECI CCNNGG 2 cut(s) 176, 810
BssMI GATC 4 cut(s) 154, 490, 570, 655
BssT1I CCWWGG 1 cut(s) 810
Bst2UI CCWGG 1 cut(s) 861
Bst4CI ACNGT 2 cut(s) 62, 238
Bst6I CTCTTC 2 cut(s) 479, 600
BstAPI GCANNNNNTGC 2 cut(s) 251, 408
BstC8I GCNNGC 4 cut(s) 18, 400, 511, 564
BstDEI CTNAG 3 cut(s) 27, 351, 753
BstF5I GGATG 6 cut(s) 174, 285, 295, 325, 402, 530
BstKTI GATC 4 cut(s) 157, 493, 573, 658
BstMBI GATC 4 cut(s) 154, 490, 570, 655
BstMWI GCNNNNNNNGC 6 cut(s) 135, 251, 408, 551, 638, 698
BstNI CCWGG 1 cut(s) 861
BstNSI RCATGY 1 cut(s) 500
BstSCI CCNGG 1 cut(s) 859
BstV1I GCAGC 2 cut(s) 218, 464
BstV2I GAAGAC 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 490
BstXI CCANNNNNNTGG 1 cut(s) 584
BstYI RGATCY 1 cut(s) 490
BsuI GTATCC 1 cut(s) 532
BsuRI GGCC 1 cut(s) 767
BtsCI GGATG 6 cut(s) 174, 285, 295, 325, 402, 530
BtsIMutI CAGTG 2 cut(s) 566, 582
Cac8I GCNNGC 4 cut(s) 18, 400, 511, 564
Cfr13I GGNCC 1 cut(s) 172
CseI GACGC 1 cut(s) 55
CsiI ACCWGGT 1 cut(s) 859
Csp6I GTAC 1 cut(s) 132
CviAII CATG 4 cut(s) 103, 497, 782, 790
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 3 cut(s) 27, 351, 753
DpnI GATC 4 cut(s) 156, 492, 572, 657
DpnII GATC 4 cut(s) 154, 490, 570, 655
DraIII CACNNNGTG 1 cut(s) 863
Eam1104I CTCTTC 2 cut(s) 479, 600
EarI CTCTTC 2 cut(s) 479, 600
Eco130I CCWWGG 1 cut(s) 810
Eco24I GRGCYC 1 cut(s) 564
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 5 cut(s) 377, 489, 576, 624, 743
EcoRI GAATTC 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 859
EcoT14I CCWWGG 1 cut(s) 810
EcoT22I ATGCAT 2 cut(s) 256, 535
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 810
FaeI CATG 4 cut(s) 106, 500, 785, 793
FalI AAGNNNNNCTT 4 cut(s) 597, 629, 630, 662
FatI CATG 4 cut(s) 102, 496, 781, 789
FauI CCCGC 1 cut(s) 82
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 3 cut(s) 14, 207, 453
FokI GGATG 6 cut(s) 181, 292, 302, 332, 389, 537
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 3 cut(s) 14, 207, 453
FspBI CTAG 1 cut(s) 693
GluI GCNGC 3 cut(s) 14, 207, 453
HaeIII GGCC 1 cut(s) 767
HapII CCGG 2 cut(s) 96, 126
HgaI GACGC 1 cut(s) 55
Hin1II CATG 4 cut(s) 106, 500, 785, 793
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 2 cut(s) 785, 841
HpaI GTTAAC 1 cut(s) 58
HpaII CCGG 2 cut(s) 96, 126
HphI GGTGA 2 cut(s) 46, 875
Hpy166II GTNNAC 2 cut(s) 58, 676
Hpy188I TCNGA 4 cut(s) 154, 666, 715, 754
Hpy188III TCNNGA 4 cut(s) 198, 614, 626, 653
Hpy8I GTNNAC 2 cut(s) 58, 676
HpyAV CCTTC 6 cut(s) 31, 84, 278, 401, 467, 551
HpyCH4III ACNGT 2 cut(s) 62, 238
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 6 cut(s) 206, 254, 301, 503, 509, 533
HpyF10VI GCNNNNNNNGC 6 cut(s) 135, 251, 408, 551, 638, 698
HpyF3I CTNAG 3 cut(s) 27, 351, 753
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 4 cut(s) 106, 500, 785, 793
Ksp22I TGATCA 1 cut(s) 154
KspAI GTTAAC 1 cut(s) 58
Kzo9I GATC 4 cut(s) 154, 490, 570, 655
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 3 cut(s) 118, 588, 854
Lsp1109I GCAGC 2 cut(s) 218, 464
LweI GCATC 6 cut(s) 241, 263, 310, 411, 512, 619
MabI ACCWGGT 1 cut(s) 859
MaeI CTAG 1 cut(s) 693
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 1 cut(s) 140
MalI GATC 4 cut(s) 156, 492, 572, 657
MbiI CCGCTC 1 cut(s) 20
MboI GATC 4 cut(s) 154, 490, 570, 655
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 2 cut(s) 564, 859
MluCI AATT 9 cut(s) 210, 226, 302, 383, 517, 709, 747, 818, 867
MlyI GAGTC 1 cut(s) 794
MmeI TCCRAC 2 cut(s) 255, 738
Mph1103I ATGCAT 2 cut(s) 256, 535
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 2 cut(s) 57, 593
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 2 cut(s) 96, 126
MspR9I CCNGG 1 cut(s) 861
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 1 cut(s) 861
MwoI GCNNNNNNNGC 6 cut(s) 135, 251, 408, 551, 638, 698
NdeII GATC 4 cut(s) 154, 490, 570, 655
NlaIII CATG 4 cut(s) 106, 500, 785, 793
NlaIV GGNNCC 4 cut(s) 94, 100, 174, 492
NmeAIII GCCGAG 1 cut(s) 157
NsiI ATGCAT 2 cut(s) 256, 535
NspI RCATGY 1 cut(s) 500
PciSI GCTCTTC 1 cut(s) 600
PctI GAATGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 1 cut(s) 841
PkrI GCNGC 3 cut(s) 15, 208, 454
PleI GAGTC 1 cut(s) 793
PpsI GAGTC 1 cut(s) 793
Psp6I CCWGG 1 cut(s) 859
PspGI CCWGG 1 cut(s) 859
PspN4I GGNNCC 4 cut(s) 94, 100, 174, 492
PspPI GGNCC 1 cut(s) 172
PsrI GAACNNNNNNTAC 2 cut(s) 91, 123
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 2 cut(s) 57, 593
SatI GCNGC 3 cut(s) 14, 207, 453
Sau3AI GATC 4 cut(s) 154, 490, 570, 655
Sau96I GGNCC 1 cut(s) 172
SchI GAGTC 1 cut(s) 794
ScrFI CCNGG 1 cut(s) 861
SduI GDGCHC 2 cut(s) 564, 859
SexAI ACCWGGT 1 cut(s) 859
SfaNI GCATC 6 cut(s) 241, 263, 310, 411, 512, 619
SinI GGWCC 1 cut(s) 172
SmlI CTYRAG 1 cut(s) 651
SmoI CTYRAG 1 cut(s) 651
Sse9I AATT 9 cut(s) 210, 226, 302, 383, 517, 709, 747, 818, 867
SsiI CCGC 6 cut(s) 13, 20, 89, 129, 138, 494
SspI AATATT 1 cut(s) 730
SspMI CTAG 1 cut(s) 693
StyD4I CCNGG 1 cut(s) 859
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 2 cut(s) 62, 238
TaiI ACGT 1 cut(s) 663
TaqI TCGA 1 cut(s) 634
TasI AATT 9 cut(s) 210, 226, 302, 383, 517, 709, 747, 818, 867
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 1 cut(s) 841
Tru1I TTAA 2 cut(s) 57, 593
Tru9I TTAA 2 cut(s) 57, 593
TscAI CASTG 2 cut(s) 573, 589
TseI GCWGC 2 cut(s) 206, 452
TspDTI ATGAA 2 cut(s) 674, 839
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 2 cut(s) 573, 589
VpaK11BI GGWCC 1 cut(s) 172
XapI RAATTY 2 cut(s) 226, 383
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 1 cut(s) 693
Zsp2I ATGCAT 2 cut(s) 256, 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.