Rh3AG298500

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002412
Physical Location & Seq
Reverse (-)
36486421 .. 36499491
13071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG298500.1

Sequence Viewer

Length: 1677 bp
ATGTCTTGTGTGCGGCGAGCGGTAGCTAAGGTGGTGAAGGAGAAGACGCATATAGTTAACCGTTTTCTCTCTCCTTCTTCTTCAACTGCCGGAACCGGAACCATGACTACTGCTCCGTTCTTCTCCGGCGGTTCTGGCGGTTACAAGTTTTCTGATCAGCATTGGAGGATGGACCCTCGGCTCTGGTTTTTTCTATCTGGAAATGCAGCAATTATTCTTGCCATAAAGTCCAACACCGTCTTGGCAAAAGATGCATCACTTGATAATGAGGCACAGGGGATGGAAGGGGATGACACAATGCAATTACGCAGAGTTGAGGATGCTTCTGTCAAATCTAACATACATACCTCTAAGTGGAGAGTTTTTACAGATAAAGGAAGGGAATTCTTCCTTCAGGGAAAAGTGGAGGAAGCTGAAAAGCTCTTTCTCGCAGCTTTACAGGAAGCTGAAGAAGGTTTTGGGAAGAGGGATCCGCATGTTGCATCTGCAAGCAATAATTTAGCAGAGCTCTATAGAGTTAATAAGCTATATCATAAGGCAGAGCCATTGTATTTGGAGGCCATCAGCATACTGGAGGAGTCATTTGGTCCTGAAGATATACGAGTTGGGTCTGCCTTTCATAACCTGGGTCAGTTCTATATTGTACAGCGGAAGCTGGATGAAGCCCGTGTTTGCTATGAGCGTGCTTTGAAGATTAAACGCCTTGTTCTGGGTCTTAACCATACAGGTTATGCAGATATGATGTATCATCTTGGAACGGTGCTACACCTCCAAGGAAAAGAAAAGGACTCTGAGGCCCTCGTTCAAGATTCCATTCGAATACTTGAGGATGGTGGTCAAGGAGAGTCGGTTATGTGCTTAAAGAGAATGCGATATCTTGCTCAGATATATCTGAAATCCAATCGATTTGAGGAGGCCGAGAAGGTACAAAGAAAGATCCTGCATATAATGGAAATAACAAAGGGATGGAATGCATTGGATACAGCAATAACAGCTGAAGGGCTCGCACTGATCCTCCAATCCACTGGGAGCTTAAAGGAAGCTGAAGAGCTTCTTGAAAGATGTCTTGATGCTCGAAAAGCCTTACTTCCTCAAGATCACGTTCAGATTGGTGTGAACTTGCTTCATATTGCTAGAGTGGCAATGCTCAATTCCGACCAACTGAAGAATATTGACTATCCAAAAGCAATTTCTGAGCTTGATAAGGCCAAAGAAGTTTTACATGAGTCCATGAGGATAGCACAATGTTCCTTGGATAAATTGATGAAGCAAAATAAGAAGATTCGTAGTAATGGAGCACCAGGTGAAATTGGAAAGAATGGTCAGGTGCCATTAGTCATATTGTTGCAAGCTTCTGATGCTCTTGGCCTTTTGGAGATCACAAAGAAACAATTACAAGGGTCAAAGCAGTCTGAGCATGCACCTAATATTGAAGCTGAGAATGCACTATTTAAATGTATATCTGCTTACAAAGAGTTCGTAACTGAGAGATCAATCCCTGAGTCTCCTGAAACAAAGGCCGAGTATCTTAAATGTTTGAAGCATCTTTTGAGCTTGAATAGTGATAGCACTACAGAAAGAACACAGCACTCTACTGGAGTCAATTTGCAAGATCTGAGAGACGAAATTAAGCGTCTTGAAGTTGAACTTTCTCCTCATAGGAAACGCAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

558

Amino Acids

62.68

Weight (kDa)

8.26

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_NPHP3 PF24885 120 - 234 1.2e-11 Nephrocystin-3 TPR domain
TPR_12 PF13424 130 - 193 1.2e-08 Tetratricopeptide repeat
TPR_12 PF13424 160 - 211 1.7e-07 Tetratricopeptide repeat
TPR_12 PF13424 203 - 276 7.9e-13 Tetratricopeptide repeat
TPR_10 PF13374 204 - 241 1.8e-06 Tetratricopeptide repeat
TPR_MalT PF17874 249 - 377 4.3e-06 MalT-like TPR region
TPR_12 PF13424 292 - 360 3.8e-09 Tetratricopeptide repeat
TPR_10 PF13374 328 - 367 1.6e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1327
AccBSI CCGCTC 1 cut(s) 20
AciI CCGC 6 cut(s) 13, 20, 129, 138, 473, 649
AclWI GGATC 4 cut(s) 464, 477, 931, 1006
AcsI RAATTY 2 cut(s) 383, 1671
AcuI CTGAAG 6 cut(s) 377, 468, 612, 1017, 1065, 1184
AdeI CACNNNGTG 1 cut(s) 1304
AfaI GTAC 2 cut(s) 645, 927
AfiI CCNNNNNNNGG 2 cut(s) 354, 709
AgsI TTSAA 9 cut(s) 84, 691, 806, 1058, 1433, 1540, 1558, 1640, 1646
AjnI CCWGG 2 cut(s) 624, 1300
AjuI GAANNNNNNNTTGG 4 cut(s) 441, 473, 1174, 1206
Alw21I GWGCWC 2 cut(s) 510, 1300
Alw26I GTCTC 2 cut(s) 1509, 1614
AlwI GGATC 4 cut(s) 464, 477, 931, 1006
AoxI GGCC 6 cut(s) 558, 795, 915, 1206, 1366, 1518
ApeKI GCWGC 2 cut(s) 206, 431
ApoI RAATTY 2 cut(s) 383, 1671
Asp700I GAANNNNTTC 1 cut(s) 1050
AspS9I GGNCC 3 cut(s) 172, 587, 796
AsuHPI GGTGA 2 cut(s) 46, 1316
AsuII TTCGAA 1 cut(s) 817
AvaII GGWCC 2 cut(s) 172, 587
BamHI GGATCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 1327
BanII GRGCYC 2 cut(s) 510, 1005
BbsI GAAGAC 1 cut(s) 50
Bbv12I GWGCWC 2 cut(s) 510, 1300
BbvI GCAGC 2 cut(s) 218, 443
BccI CCATC 5 cut(s) 163, 274, 569, 824, 960
BciT130I CCWGG 2 cut(s) 626, 1302
BciVI GTATCC 1 cut(s) 973
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 1509, 1614
BfaI CTAG 1 cut(s) 1134
BfmI CTRYAG 2 cut(s) 511, 1572
BfuI GTATCC 1 cut(s) 973
BglII AGATCT 1 cut(s) 1612
BisI GCNGC 3 cut(s) 14, 207, 432
BlsI GCNGC 3 cut(s) 15, 208, 433
Bme1390I CCNGG 2 cut(s) 626, 1302
Bme18I GGWCC 2 cut(s) 172, 587
BmgT120I GGNCC 3 cut(s) 172, 587, 796
BmiI GGNNCC 5 cut(s) 94, 100, 174, 471, 1329
BmrFI CCNGG 2 cut(s) 626, 1302
BmrI ACTGGG 1 cut(s) 1035
BmsI GCATC 7 cut(s) 241, 263, 310, 491, 1060, 1348, 1552
BmuI ACTGGG 1 cut(s) 1035
BpiI GAAGAC 1 cut(s) 50
BpmI CTGGAG 2 cut(s) 593, 1617
Bpu10I CCTNAGC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 817
BpuEI CTTGAG 2 cut(s) 845, 1077
Bsa29I ATCGAT 1 cut(s) 904
BsaJI CCNNGG 4 cut(s) 176, 625, 772, 1251
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 6 cut(s) 97, 127, 834, 864, 999, 1029
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 709
Bse1I ACTGG 3 cut(s) 576, 1030, 1600
Bse3DI GCAATG 1 cut(s) 1149
BseBI CCWGG 2 cut(s) 626, 1302
BseCI ATCGAT 1 cut(s) 904
BseDI CCNNGG 4 cut(s) 176, 625, 772, 1251
BseGI GGATG 7 cut(s) 174, 285, 295, 325, 664, 835, 971
BseLI CCNNNNNNNGG 2 cut(s) 354, 709
BseMI GCAATG 1 cut(s) 1149
BseMII CTCAG 8 cut(s) 783, 896, 1185, 1404, 1428, 1476, 1491, 1607
BseNI ACTGG 3 cut(s) 576, 1030, 1600
BseRI GAGGAG 3 cut(s) 590, 926, 1644
BseXI GCAGC 2 cut(s) 218, 443
BshFI GGCC 6 cut(s) 560, 797, 917, 1208, 1368, 1520
BshNI GGYRCC 1 cut(s) 1327
BshVI ATCGAT 1 cut(s) 904
BsiHKAI GWGCWC 2 cut(s) 510, 1300
BsiSI CCGG 3 cut(s) 90, 96, 126
BslI CCNNNNNNNGG 2 cut(s) 354, 709
BsmAI GTCTC 2 cut(s) 1509, 1614
BsmBI CGTCTC 1 cut(s) 1614
BsmI GAATGC 3 cut(s) 873, 976, 1447
BsnI GGCC 6 cut(s) 560, 797, 917, 1208, 1368, 1520
Bsp119I TTCGAA 1 cut(s) 817
Bsp1286I GDGCHC 3 cut(s) 510, 1005, 1300
Bsp1407I TGTACA 1 cut(s) 643
Bsp143I GATC 8 cut(s) 154, 469, 936, 1011, 1096, 1377, 1490, 1612
BspACI CCGC 6 cut(s) 13, 20, 129, 138, 473, 649
BspANI GGCC 6 cut(s) 560, 797, 917, 1208, 1368, 1520
BspCNI CTCAG 8 cut(s) 784, 895, 1186, 1405, 1429, 1477, 1492, 1608
BspDI ATCGAT 1 cut(s) 904
BspLI GGNNCC 5 cut(s) 94, 100, 174, 471, 1329
BspPI GGATC 4 cut(s) 464, 477, 931, 1006
BspQI GCTCTTC 1 cut(s) 1041
BspT104I TTCGAA 1 cut(s) 817
BspT107I GGYRCC 1 cut(s) 1327
BsrBI CCGCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 1149
BsrGI TGTACA 1 cut(s) 643
BsrI ACTGG 3 cut(s) 576, 1030, 1600
BssECI CCNNGG 4 cut(s) 176, 625, 772, 1251
BssMI GATC 8 cut(s) 154, 469, 936, 1011, 1096, 1377, 1490, 1612
BssT1I CCWWGG 2 cut(s) 772, 1251
Bst2UI CCWGG 2 cut(s) 626, 1302
Bst4CI ACNGT 3 cut(s) 62, 238, 760
Bst6I CTCTTC 2 cut(s) 458, 1041
BstAPI GCANNNNNTGC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 643
BstBI TTCGAA 1 cut(s) 817
BstC8I GCNNGC 6 cut(s) 18, 490, 684, 1005, 1350, 1419
BstF5I GGATG 7 cut(s) 174, 285, 295, 325, 664, 835, 971
BstKTI GATC 8 cut(s) 157, 472, 939, 1014, 1099, 1380, 1493, 1615
BstMAI GTCTC 2 cut(s) 1509, 1614
BstMBI GATC 8 cut(s) 154, 469, 936, 1011, 1096, 1377, 1490, 1612
BstMWI GCNNNNNNNGC 8 cut(s) 135, 251, 992, 1079, 1139, 1358, 1414, 1442
BstNI CCWGG 2 cut(s) 626, 1302
BstNSI RCATGY 2 cut(s) 479, 1421
BstSCI CCNGG 2 cut(s) 624, 1300
BstSFI CTRYAG 2 cut(s) 511, 1572
BstV1I GCAGC 2 cut(s) 218, 443
BstV2I GAAGAC 1 cut(s) 50
BstX2I RGATCY 3 cut(s) 469, 936, 1612
BstXI CCANNNNNNTGG 1 cut(s) 1025
BstYI RGATCY 3 cut(s) 469, 936, 1612
Bsu15I ATCGAT 1 cut(s) 904
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 6 cut(s) 560, 797, 917, 1208, 1368, 1520
BsuTUI ATCGAT 1 cut(s) 904
BtsCI GGATG 7 cut(s) 174, 285, 295, 325, 664, 835, 971
BtsIMutI CAGTG 2 cut(s) 1007, 1023
Cac8I GCNNGC 6 cut(s) 18, 490, 684, 1005, 1350, 1419
Cfr13I GGNCC 3 cut(s) 172, 587, 796
ClaI ATCGAT 1 cut(s) 904
CseI GACGC 2 cut(s) 55, 1622
CsiI ACCWGGT 1 cut(s) 1300
Csp6I GTAC 2 cut(s) 644, 926
CviAII CATG 5 cut(s) 103, 476, 1223, 1231, 1418
CviQI GTAC 2 cut(s) 644, 926
DpnI GATC 8 cut(s) 156, 471, 938, 1013, 1098, 1379, 1492, 1614
DpnII GATC 8 cut(s) 154, 469, 936, 1011, 1096, 1377, 1490, 1612
DraI TTTAAA 1 cut(s) 1453
DraIII CACNNNGTG 1 cut(s) 1304
Eam1104I CTCTTC 2 cut(s) 458, 1041
EarI CTCTTC 2 cut(s) 458, 1041
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 2 cut(s) 772, 1251
Eco24I GRGCYC 2 cut(s) 510, 1005
Eco32I GATATC 1 cut(s) 875
Eco47I GGWCC 2 cut(s) 172, 587
Eco53kI GAGCTC 1 cut(s) 508
Eco57I CTGAAG 6 cut(s) 377, 468, 612, 1017, 1065, 1184
EcoICRI GAGCTC 1 cut(s) 508
EcoO109I RGGNCCY 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 383
EcoRII CCWGG 2 cut(s) 624, 1300
EcoRV GATATC 1 cut(s) 875
EcoT14I CCWWGG 2 cut(s) 772, 1251
EcoT22I ATGCAT 2 cut(s) 256, 976
EcoT38I GRGCYC 2 cut(s) 510, 1005
ErhI CCWWGG 2 cut(s) 772, 1251
Esp3I CGTCTC 1 cut(s) 1614
FaeI CATG 5 cut(s) 106, 479, 1226, 1234, 1421
FalI AAGNNNNNCTT 6 cut(s) 1038, 1070, 1071, 1103, 1632, 1664
FatI CATG 5 cut(s) 102, 475, 1222, 1230, 1417
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 3 cut(s) 14, 207, 432
FokI GGATG 7 cut(s) 181, 292, 302, 332, 671, 842, 978
FriOI GRGCYC 2 cut(s) 510, 1005
Fsp4HI GCNGC 3 cut(s) 14, 207, 432
FspBI CTAG 1 cut(s) 1134
GluI GCNGC 3 cut(s) 14, 207, 432
GsuI CTGGAG 2 cut(s) 593, 1617
HaeIII GGCC 6 cut(s) 560, 797, 917, 1208, 1368, 1520
HapII CCGG 3 cut(s) 90, 96, 126
HgaI GACGC 2 cut(s) 55, 1622
Hin1II CATG 5 cut(s) 106, 479, 1226, 1234, 1421
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 1 cut(s) 1350
HinfI GANTC 8 cut(s) 578, 788, 809, 845, 1226, 1282, 1502, 1599
HpaI GTTAAC 1 cut(s) 58
HpaII CCGG 3 cut(s) 90, 96, 126
HphI GGTGA 2 cut(s) 46, 1316
Hpy166II GTNNAC 2 cut(s) 58, 1117
Hpy188III TCNNGA 8 cut(s) 198, 590, 806, 1055, 1067, 1094, 1508, 1637
Hpy8I GTNNAC 2 cut(s) 58, 1117
HpyAV CCTTC 8 cut(s) 31, 84, 278, 372, 401, 446, 916, 992
HpyCH4III ACNGT 3 cut(s) 62, 238, 760
HpyCH4IV ACGT 1 cut(s) 1101
HpyF10VI GCNNNNNNNGC 8 cut(s) 135, 251, 992, 1079, 1139, 1358, 1414, 1442
HpySE526I ACGT 1 cut(s) 1101
Hsp92II CATG 5 cut(s) 106, 479, 1226, 1234, 1421
Ksp22I TGATCA 1 cut(s) 154
KspAI GTTAAC 1 cut(s) 58
Kzo9I GATC 8 cut(s) 154, 469, 936, 1011, 1096, 1377, 1490, 1612
LguI GCTCTTC 1 cut(s) 1041
LmnI GCTCC 3 cut(s) 118, 1029, 1295
Lsp1109I GCAGC 2 cut(s) 218, 443
LweI GCATC 7 cut(s) 241, 263, 310, 491, 1060, 1348, 1552
MabI ACCWGGT 1 cut(s) 1300
MaeI CTAG 1 cut(s) 1134
MaeII ACGT 1 cut(s) 1101
MaeIII GTNAC 2 cut(s) 140, 1480
MalI GATC 8 cut(s) 156, 471, 938, 1013, 1098, 1379, 1492, 1614
MbiI CCGCTC 1 cut(s) 20
MboI GATC 8 cut(s) 154, 469, 936, 1011, 1096, 1377, 1490, 1612
MflI RGATCY 3 cut(s) 469, 936, 1612
MhlI GDGCHC 3 cut(s) 510, 1005, 1300
MlyI GAGTC 6 cut(s) 587, 782, 854, 1235, 1511, 1608
MmeI TCCRAC 2 cut(s) 255, 1179
Mph1103I ATGCAT 2 cut(s) 256, 976
MroXI GAANNNNTTC 1 cut(s) 1050
MseI TTAA 9 cut(s) 57, 519, 696, 717, 860, 1034, 1452, 1530, 1629
MspA1I CMGCKG 2 cut(s) 649, 995
MspI CCGG 3 cut(s) 90, 96, 126
MspR9I CCNGG 2 cut(s) 626, 1302
Mva1269I GAATGC 3 cut(s) 873, 976, 1447
MvaI CCWGG 2 cut(s) 626, 1302
MwoI GCNNNNNNNGC 8 cut(s) 135, 251, 992, 1079, 1139, 1358, 1414, 1442
NdeII GATC 8 cut(s) 154, 469, 936, 1011, 1096, 1377, 1490, 1612
NlaIII CATG 5 cut(s) 106, 479, 1226, 1234, 1421
NlaIV GGNNCC 5 cut(s) 94, 100, 174, 471, 1329
NmeAIII GCCGAG 3 cut(s) 157, 943, 1546
NsiI ATGCAT 2 cut(s) 256, 976
NspI RCATGY 2 cut(s) 479, 1421
NspV TTCGAA 1 cut(s) 817
PaeI GCATGC 1 cut(s) 1421
PciSI GCTCTTC 1 cut(s) 1041
PctI GAATGC 3 cut(s) 873, 976, 1447
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 2 cut(s) 809, 1282
PkrI GCNGC 3 cut(s) 15, 208, 433
PleI GAGTC 6 cut(s) 586, 782, 853, 1234, 1510, 1607
PpsI GAGTC 6 cut(s) 586, 782, 853, 1234, 1510, 1607
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 2 cut(s) 624, 1300
PspGI CCWGG 2 cut(s) 624, 1300
PspN4I GGNNCC 5 cut(s) 94, 100, 174, 471, 1329
PspPI GGNCC 3 cut(s) 172, 587, 796
PsrI GAACNNNNNNTAC 2 cut(s) 91, 123
PsuI RGATCY 3 cut(s) 469, 936, 1612
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 2 cut(s) 645, 927
RsaNI GTAC 2 cut(s) 644, 926
SacI GAGCTC 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 1041
SaqAI TTAA 9 cut(s) 57, 519, 696, 717, 860, 1034, 1452, 1530, 1629
SatI GCNGC 3 cut(s) 14, 207, 432
Sau3AI GATC 8 cut(s) 154, 469, 936, 1011, 1096, 1377, 1490, 1612
Sau96I GGNCC 3 cut(s) 172, 587, 796
SchI GAGTC 6 cut(s) 587, 782, 854, 1235, 1511, 1608
ScrFI CCNGG 2 cut(s) 626, 1302
SduI GDGCHC 3 cut(s) 510, 1005, 1300
SexAI ACCWGGT 1 cut(s) 1300
SfaNI GCATC 7 cut(s) 241, 263, 310, 491, 1060, 1348, 1552
SfcI CTRYAG 2 cut(s) 511, 1572
SfuI TTCGAA 1 cut(s) 817
SinI GGWCC 2 cut(s) 172, 587
SmiI ATTTAAAT 1 cut(s) 1453
SmlI CTYRAG 2 cut(s) 824, 1092
SmoI CTYRAG 2 cut(s) 824, 1092
SphI GCATGC 1 cut(s) 1421
SsiI CCGC 6 cut(s) 13, 20, 129, 138, 473, 649
SspI AATATT 2 cut(s) 1171, 1429
SspMI CTAG 1 cut(s) 1134
SstI GAGCTC 1 cut(s) 510
StyD4I CCNGG 2 cut(s) 624, 1300
StyI CCWWGG 2 cut(s) 772, 1251
SwaI ATTTAAAT 1 cut(s) 1453
TaaI ACNGT 3 cut(s) 62, 238, 760
TaiI ACGT 1 cut(s) 1104
TaqI TCGA 3 cut(s) 817, 904, 1075
TatI WGTACW 1 cut(s) 643
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 2 cut(s) 809, 1282
Tru1I TTAA 9 cut(s) 57, 519, 696, 717, 860, 1034, 1452, 1530, 1629
Tru9I TTAA 9 cut(s) 57, 519, 696, 717, 860, 1034, 1452, 1530, 1629
TscAI CASTG 2 cut(s) 1014, 1030
TseI GCWGC 2 cut(s) 206, 431
TspDTI ATGAA 4 cut(s) 608, 675, 1115, 1280
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 2 cut(s) 1014, 1030
VpaK11BI GGWCC 2 cut(s) 172, 587
XapI RAATTY 2 cut(s) 383, 1671
XceI RCATGY 2 cut(s) 479, 1421
XcmI CCANNNNNNNNNTGG 2 cut(s) 238, 568
XmnI GAANNNNTTC 1 cut(s) 1050
XspI CTAG 1 cut(s) 1134
Zsp2I ATGCAT 2 cut(s) 256, 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.