Rh3BG334300

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002412
Physical Location & Seq
Reverse (-)
35424603 .. 35437942
13340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG334300.1

Sequence Viewer

Length: 1608 bp
ATGTCGTGTGTGCGGCGAGCGGCAGCTAAGGTGGTGAAGGAGAAGAGGCATATAATTAACCGTTTTCTCTCTCCTTCTTCTTCAACTGCCGGAACCGGAACCAGAACCGAAACCATCCCTACTGCTCCGTTCTTCTCCGGCGGTACTGGTACGGAATCAATCGCCAAAACAAACAAACAAACAAGTAAGCTAAATCCGCCTAATTCAACTGACTCACTAGTGTTGCTCGGTTCCAAGTTTTCTGATCAGCATTGGAGGATCGACCCTCGTCTCTGGTTTTTTCTATCTGGAAATGCAGCAATTATTCTTGCCATAAATTCCAACACCGTCTTGGCAAAAGATGCATCACTTGATAATGAGGTGCAGGGGATGGAAGGGGATGACACAATGCAATTACGCAGAGTTGAGGATGCTTCTGTCAAATCTAACATACATACCTCTAAGTGGAGAGTTTTTACAGATAAAGGGAGGGAATTCTTCCTTCAGGGAAAAGTGGAGGAAGCTGAAAAGCTCTTTCTCGCGGCTTTACAGGAAGCTAAAGAAGGTTTTGGGAAGAGGGATCCGCATGTTGCATCTGCAAGCAACAATTTAGCAGAGCTCTATAGAGTTAATAAGCTATATCATAAGGCAGAGCCATTGTATTTGGAGGCCATCAGCATACTGGAGGAGTCATTTGGTCCTGAAGATATACGAGTTGGGTCTGCCTTTCATAATCTGGGTCAGTTCTATATTGTACAGCGGAAGCTGGATGAAGCCCGTGTTTGCTATGAGCGTGCTTTGAAGATTAAACGCCGTGTTCTGGGTCTTAACCATACGGGTTATGCAGATACTATGTATCATCTTGGAACGGTGCTACACCTCCAAGGAAAAGAAAAGGACTCTGAGGCCCTCGTTCAAGATTCCATTCGAATACTTGAGGATGGTGGTCAAGGAGAGTCGGTTATGTGCTTAAAGAGAATGCGATATCTTGCTCAGATTTATCTGAAATCCAATCGATTTGAGGAGGCCGAGAAGGTACAAAGAAAGATCTTGCATATAATGGAAATAACAAAGGGATGGAATGCATTGGATACAGCAATAACAGCTGAAGGGCTCGCACTGATCCTCCAATCCACTGGGAGCTTAAAGGAAGCTGAAGAGCTTCTTGAAAGATGTCTTGATGCTCGAAAAGCCTTACTTCCTCAAGATCACATTCAGATTGGTGTGAACTTGCTTCATATTGCTAGAGTGGCAATGCTCAATTCTGACCAACTGAAGAATATTGACTATCCAAAAGCAATTTCTGAGCTTGATAAGGCCAAAGAAGTTTTACATGAGTCCATGAGGATAGCACAGTGTTCCTTGGATAAATTGATGAAGCAAAATAAGAAGATTCGTAGTAATGGAGCACCAGGTGAAATGGGAAAGAATGGTCAGGTGCCGTTAGTCATATTGTTGCAAGCTTCTGATGCTCTTGGCCTTTTGGAGATCACAAAGAAACAATTAAAAGGGTCAAAGTCTGAGCATGCACCTAATATCGAAGCTGAGAATGCACTATTTAAATGTATATCTGCTTACAGAGAGGTCCCAATAAATTTTTGGAAAGTAGTCGATCTTTTTGGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

535

Amino Acids

59.85

Weight (kDa)

8.83

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 160 - 223 1.8e-08 Tetratricopeptide repeat
TPR_NPHP3 PF24885 184 - 266 1.4e-11 Nephrocystin-3 TPR domain
TPR_12 PF13424 190 - 241 1.6e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 232 - 348 1.1e-08 Nephrocystin-3 TPR domain
TPR_12 PF13424 233 - 306 1.4e-14 Tetratricopeptide repeat
TPR_10 PF13374 234 - 271 4e-08 Tetratricopeptide repeat
TPR_MalT PF17874 278 - 395 3.1e-06 MalT-like TPR region
TPR_12 PF13424 322 - 390 3.6e-09 Tetratricopeptide repeat
TPR_10 PF13374 358 - 398 1.2e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1417
AccBSI CCGCTC 1 cut(s) 20
AccII CGCG 1 cut(s) 521
AciI CCGC 7 cut(s) 13, 20, 141, 197, 521, 563, 739
AclWI GGATC 4 cut(s) 266, 554, 567, 1096
AcsI RAATTY 3 cut(s) 316, 473, 1573
AcuI CTGAAG 5 cut(s) 467, 702, 1107, 1155, 1274
AdeI CACNNNGTG 1 cut(s) 1394
AfaI GTAC 5 cut(s) 145, 151, 735, 1017, 1604
AfiI CCNNNNNNNGG 2 cut(s) 444, 799
AgsI TTSAA 5 cut(s) 84, 207, 781, 896, 1148
AhlI ACTAGT 1 cut(s) 217
AjnI CCWGG 1 cut(s) 1390
AjuI GAANNNNNNNTTGG 2 cut(s) 1264, 1296
Alw21I GWGCWC 2 cut(s) 600, 1390
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 4 cut(s) 266, 554, 567, 1096
AoxI GGCC 5 cut(s) 648, 885, 1005, 1296, 1456
ApeKI GCWGC 2 cut(s) 23, 296
ApoI RAATTY 3 cut(s) 316, 473, 1573
Asp700I GAANNNNTTC 1 cut(s) 1140
AspS9I GGNCC 3 cut(s) 677, 886, 1564
AsuHPI GGTGA 2 cut(s) 46, 1406
AsuII TTCGAA 1 cut(s) 907
AvaII GGWCC 2 cut(s) 677, 1564
BamHI GGATCC 1 cut(s) 559
BanI GGYRCC 1 cut(s) 1417
BanII GRGCYC 2 cut(s) 600, 1095
Bbv12I GWGCWC 2 cut(s) 600, 1390
BbvI GCAGC 2 cut(s) 35, 308
BccI CCATC 5 cut(s) 122, 364, 659, 914, 1050
BceAI ACGGC 2 cut(s) 777, 1405
BciT130I CCWGG 1 cut(s) 1392
BciVI GTATCC 1 cut(s) 1063
BclI TGATCA 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 275
BcuI ACTAGT 1 cut(s) 217
BfaI CTAG 2 cut(s) 218, 1224
BfmI CTRYAG 1 cut(s) 601
BfuI GTATCC 1 cut(s) 1063
BglII AGATCT 1 cut(s) 1026
BisI GCNGC 5 cut(s) 14, 21, 24, 297, 522
BlsI GCNGC 5 cut(s) 15, 22, 25, 298, 523
Bme1390I CCNGG 1 cut(s) 1392
Bme18I GGWCC 2 cut(s) 677, 1564
BmgT120I GGNCC 3 cut(s) 677, 886, 1564
BmiI GGNNCC 6 cut(s) 94, 100, 232, 561, 1419, 1566
BmrFI CCNGG 1 cut(s) 1392
BmrI ACTGGG 1 cut(s) 1125
BmsI GCATC 6 cut(s) 331, 353, 400, 581, 1150, 1438
BmuI ACTGGG 1 cut(s) 1125
BoxI GACNNNNGTC 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 683
Bpu10I CCTNAGC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 907
BpuEI CTTGAG 2 cut(s) 935, 1167
Bsa29I ATCGAT 1 cut(s) 994
BsaJI CCNNGG 2 cut(s) 862, 1341
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 4 cut(s) 924, 954, 1089, 1119
Bsc4I CCNNNNNNNGG 2 cut(s) 444, 799
Bse1I ACTGG 3 cut(s) 151, 666, 1120
Bse3DI GCAATG 1 cut(s) 1239
BseBI CCWGG 1 cut(s) 1392
BseCI ATCGAT 1 cut(s) 994
BseDI CCNNGG 2 cut(s) 862, 1341
BseGI GGATG 7 cut(s) 114, 375, 385, 415, 754, 925, 1061
BseLI CCNNNNNNNGG 2 cut(s) 444, 799
BseMI GCAATG 1 cut(s) 1239
BseMII CTCAG 5 cut(s) 873, 986, 1275, 1491, 1515
BseNI ACTGG 3 cut(s) 151, 666, 1120
BseRI GAGGAG 2 cut(s) 680, 1016
BseXI GCAGC 2 cut(s) 35, 308
BsgI GTGCAG 1 cut(s) 383
Bsh1236I CGCG 1 cut(s) 521
BshFI GGCC 5 cut(s) 650, 887, 1007, 1298, 1458
BshNI GGYRCC 1 cut(s) 1417
BshVI ATCGAT 1 cut(s) 994
BsiHKAI GWGCWC 2 cut(s) 600, 1390
BsiSI CCGG 3 cut(s) 90, 96, 138
BslFI GGGAC 1 cut(s) 1550
BslI CCNNNNNNNGG 2 cut(s) 444, 799
BsmAI GTCTC 1 cut(s) 275
BsmBI CGTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 1550
BsmI GAATGC 3 cut(s) 963, 1066, 1534
BsnI GGCC 5 cut(s) 650, 887, 1007, 1298, 1458
Bsp119I TTCGAA 1 cut(s) 907
Bsp1286I GDGCHC 3 cut(s) 600, 1095, 1390
Bsp1407I TGTACA 1 cut(s) 733
Bsp143I GATC 8 cut(s) 244, 258, 559, 1026, 1101, 1186, 1467, 1591
BspACI CCGC 7 cut(s) 13, 20, 141, 197, 521, 563, 739
BspANI GGCC 5 cut(s) 650, 887, 1007, 1298, 1458
BspCNI CTCAG 5 cut(s) 874, 985, 1276, 1492, 1516
BspDI ATCGAT 1 cut(s) 994
BspFNI CGCG 1 cut(s) 521
BspLI GGNNCC 6 cut(s) 94, 100, 232, 561, 1419, 1566
BspPI GGATC 4 cut(s) 266, 554, 567, 1096
BspQI GCTCTTC 1 cut(s) 1131
BspT104I TTCGAA 1 cut(s) 907
BspT107I GGYRCC 1 cut(s) 1417
BsrBI CCGCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 1239
BsrGI TGTACA 1 cut(s) 733
BsrI ACTGG 3 cut(s) 151, 666, 1120
BssECI CCNNGG 2 cut(s) 862, 1341
BssMI GATC 8 cut(s) 244, 258, 559, 1026, 1101, 1186, 1467, 1591
BssT1I CCWWGG 2 cut(s) 862, 1341
Bst2UI CCWGG 1 cut(s) 1392
Bst4CI ACNGT 4 cut(s) 62, 328, 850, 1335
Bst6I CTCTTC 3 cut(s) 38, 548, 1131
BstAPI GCANNNNNTGC 1 cut(s) 341
BstAUI TGTACA 1 cut(s) 733
BstBI TTCGAA 1 cut(s) 907
BstC8I GCNNGC 6 cut(s) 18, 580, 774, 1095, 1440, 1506
BstDEI CTNAG 7 cut(s) 27, 441, 882, 972, 1284, 1500, 1524
BstF5I GGATG 7 cut(s) 114, 375, 385, 415, 754, 925, 1061
BstFNI CGCG 1 cut(s) 521
BstKTI GATC 8 cut(s) 247, 261, 562, 1029, 1104, 1189, 1470, 1594
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 8 cut(s) 244, 258, 559, 1026, 1101, 1186, 1467, 1591
BstMWI GCNNNNNNNGC 7 cut(s) 196, 341, 1082, 1169, 1229, 1448, 1529
BstNI CCWGG 1 cut(s) 1392
BstNSI RCATGY 2 cut(s) 569, 1508
BstPAI GACNNNNGTC 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 1390
BstSFI CTRYAG 1 cut(s) 601
BstUI CGCG 1 cut(s) 521
BstV1I GCAGC 2 cut(s) 35, 308
BstX2I RGATCY 2 cut(s) 559, 1026
BstXI CCANNNNNNTGG 1 cut(s) 1115
BstYI RGATCY 2 cut(s) 559, 1026
Bsu15I ATCGAT 1 cut(s) 994
BsuI GTATCC 1 cut(s) 1063
BsuRI GGCC 5 cut(s) 650, 887, 1007, 1298, 1458
BsuTUI ATCGAT 1 cut(s) 994
BtsCI GGATG 7 cut(s) 114, 375, 385, 415, 754, 925, 1061
BtsIMutI CAGTG 3 cut(s) 1097, 1113, 1340
Cac8I GCNNGC 6 cut(s) 18, 580, 774, 1095, 1440, 1506
Cfr13I GGNCC 3 cut(s) 677, 886, 1564
ClaI ATCGAT 1 cut(s) 994
CsiI ACCWGGT 1 cut(s) 1390
Csp6I GTAC 5 cut(s) 144, 150, 734, 1016, 1603
CviAII CATG 4 cut(s) 566, 1313, 1321, 1505
CviQI GTAC 5 cut(s) 144, 150, 734, 1016, 1603
DdeI CTNAG 7 cut(s) 27, 441, 882, 972, 1284, 1500, 1524
DpnI GATC 8 cut(s) 246, 260, 561, 1028, 1103, 1188, 1469, 1593
DpnII GATC 8 cut(s) 244, 258, 559, 1026, 1101, 1186, 1467, 1591
DraI TTTAAA 1 cut(s) 1540
DraIII CACNNNGTG 1 cut(s) 1394
Eam1104I CTCTTC 3 cut(s) 38, 548, 1131
EarI CTCTTC 3 cut(s) 38, 548, 1131
EciI GGCGGA 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 2 cut(s) 862, 1341
Eco24I GRGCYC 2 cut(s) 600, 1095
Eco32I GATATC 1 cut(s) 965
Eco47I GGWCC 2 cut(s) 677, 1564
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 5 cut(s) 467, 702, 1107, 1155, 1274
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 2 cut(s) 886, 1564
EcoRI GAATTC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 1390
EcoRV GATATC 1 cut(s) 965
EcoT14I CCWWGG 2 cut(s) 862, 1341
EcoT22I ATGCAT 2 cut(s) 346, 1066
EcoT38I GRGCYC 2 cut(s) 600, 1095
ErhI CCWWGG 2 cut(s) 862, 1341
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 4 cut(s) 569, 1316, 1324, 1508
FalI AAGNNNNNCTT 4 cut(s) 1128, 1160, 1161, 1193
FaqI GGGAC 1 cut(s) 1550
FatI CATG 4 cut(s) 565, 1312, 1320, 1504
FbaI TGATCA 1 cut(s) 244
Fnu4HI GCNGC 5 cut(s) 14, 21, 24, 297, 522
FokI GGATG 7 cut(s) 101, 382, 392, 422, 761, 932, 1068
FriOI GRGCYC 2 cut(s) 600, 1095
Fsp4HI GCNGC 5 cut(s) 14, 21, 24, 297, 522
FspBI CTAG 2 cut(s) 218, 1224
GluI GCNGC 5 cut(s) 14, 21, 24, 297, 522
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 5 cut(s) 650, 887, 1007, 1298, 1458
HapII CCGG 3 cut(s) 90, 96, 138
Hin1II CATG 4 cut(s) 569, 1316, 1324, 1508
HindIII AAGCTT 1 cut(s) 1440
HinfI GANTC 8 cut(s) 155, 212, 668, 878, 899, 935, 1316, 1372
HpaII CCGG 3 cut(s) 90, 96, 138
HphI GGTGA 2 cut(s) 46, 1406
Hpy166II GTNNAC 1 cut(s) 1207
Hpy188I TCNGA 9 cut(s) 244, 883, 975, 984, 1197, 1246, 1285, 1447, 1501
Hpy188III TCNNGA 6 cut(s) 288, 680, 896, 1145, 1157, 1184
Hpy8I GTNNAC 1 cut(s) 1207
HpyAV CCTTC 7 cut(s) 31, 84, 368, 491, 536, 1006, 1082
HpyCH4III ACNGT 4 cut(s) 62, 328, 850, 1335
HpyF10VI GCNNNNNNNGC 7 cut(s) 196, 341, 1082, 1169, 1229, 1448, 1529
HpyF3I CTNAG 7 cut(s) 27, 441, 882, 972, 1284, 1500, 1524
Hsp92II CATG 4 cut(s) 569, 1316, 1324, 1508
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 8 cut(s) 244, 258, 559, 1026, 1101, 1186, 1467, 1591
LguI GCTCTTC 1 cut(s) 1131
LmnI GCTCC 3 cut(s) 130, 1119, 1385
Lsp1109I GCAGC 2 cut(s) 35, 308
LweI GCATC 6 cut(s) 331, 353, 400, 581, 1150, 1438
MabI ACCWGGT 1 cut(s) 1390
MaeI CTAG 2 cut(s) 218, 1224
MalI GATC 8 cut(s) 246, 260, 561, 1028, 1103, 1188, 1469, 1593
MbiI CCGCTC 1 cut(s) 20
MboI GATC 8 cut(s) 244, 258, 559, 1026, 1101, 1186, 1467, 1591
MflI RGATCY 2 cut(s) 559, 1026
MhlI GDGCHC 3 cut(s) 600, 1095, 1390
MlyI GAGTC 5 cut(s) 206, 677, 872, 944, 1325
MmeI TCCRAC 1 cut(s) 345
Mph1103I ATGCAT 2 cut(s) 346, 1066
MroXI GAANNNNTTC 1 cut(s) 1140
MseI TTAA 8 cut(s) 57, 609, 786, 807, 950, 1124, 1484, 1539
MspA1I CMGCKG 2 cut(s) 739, 1085
MspI CCGG 3 cut(s) 90, 96, 138
MspR9I CCNGG 1 cut(s) 1392
Mva1269I GAATGC 3 cut(s) 963, 1066, 1534
MvaI CCWGG 1 cut(s) 1392
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 7 cut(s) 196, 341, 1082, 1169, 1229, 1448, 1529
NdeII GATC 8 cut(s) 244, 258, 559, 1026, 1101, 1186, 1467, 1591
NlaIII CATG 4 cut(s) 569, 1316, 1324, 1508
NlaIV GGNNCC 6 cut(s) 94, 100, 232, 561, 1419, 1566
NmeAIII GCCGAG 1 cut(s) 1033
NsiI ATGCAT 2 cut(s) 346, 1066
NspI RCATGY 2 cut(s) 569, 1508
NspV TTCGAA 1 cut(s) 907
PaeI GCATGC 1 cut(s) 1508
PciSI GCTCTTC 1 cut(s) 1131
PctI GAATGC 3 cut(s) 963, 1066, 1534
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 3 cut(s) 155, 899, 1372
PkrI GCNGC 5 cut(s) 15, 22, 25, 298, 523
PleI GAGTC 5 cut(s) 206, 676, 872, 943, 1324
PpsI GAGTC 5 cut(s) 206, 676, 872, 943, 1324
PpuMI RGGWCCY 1 cut(s) 1564
PshAI GACNNNNGTC 1 cut(s) 267
Psp124BI GAGCTC 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 1564
Psp6I CCWGG 1 cut(s) 1390
PspGI CCWGG 1 cut(s) 1390
PspN4I GGNNCC 6 cut(s) 94, 100, 232, 561, 1419, 1566
PspPI GGNCC 3 cut(s) 677, 886, 1564
PspPPI RGGWCCY 1 cut(s) 1564
PsuI RGATCY 2 cut(s) 559, 1026
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 5 cut(s) 145, 151, 735, 1017, 1604
RsaNI GTAC 5 cut(s) 144, 150, 734, 1016, 1603
SacI GAGCTC 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 1131
SaqAI TTAA 8 cut(s) 57, 609, 786, 807, 950, 1124, 1484, 1539
SatI GCNGC 5 cut(s) 14, 21, 24, 297, 522
Sau3AI GATC 8 cut(s) 244, 258, 559, 1026, 1101, 1186, 1467, 1591
Sau96I GGNCC 3 cut(s) 677, 886, 1564
SchI GAGTC 5 cut(s) 206, 677, 872, 944, 1325
ScrFI CCNGG 1 cut(s) 1392
SduI GDGCHC 3 cut(s) 600, 1095, 1390
SexAI ACCWGGT 1 cut(s) 1390
SfaNI GCATC 6 cut(s) 331, 353, 400, 581, 1150, 1438
SfcI CTRYAG 1 cut(s) 601
SfuI TTCGAA 1 cut(s) 907
SinI GGWCC 2 cut(s) 677, 1564
SmiI ATTTAAAT 1 cut(s) 1540
SmlI CTYRAG 2 cut(s) 914, 1182
SmoI CTYRAG 2 cut(s) 914, 1182
SpeI ACTAGT 1 cut(s) 217
SphI GCATGC 1 cut(s) 1508
SsiI CCGC 7 cut(s) 13, 20, 141, 197, 521, 563, 739
SspI AATATT 1 cut(s) 1261
SspMI CTAG 2 cut(s) 218, 1224
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 1 cut(s) 1390
StyI CCWWGG 2 cut(s) 862, 1341
SwaI ATTTAAAT 1 cut(s) 1540
TaaI ACNGT 4 cut(s) 62, 328, 850, 1335
TaqI TCGA 6 cut(s) 261, 907, 994, 1165, 1518, 1590
TatI WGTACW 1 cut(s) 733
TauI GCSGC 3 cut(s) 16, 23, 524
TfiI GAWTC 3 cut(s) 155, 899, 1372
Tru1I TTAA 8 cut(s) 57, 609, 786, 807, 950, 1124, 1484, 1539
Tru9I TTAA 8 cut(s) 57, 609, 786, 807, 950, 1124, 1484, 1539
TscAI CASTG 3 cut(s) 1104, 1120, 1340
TseI GCWGC 2 cut(s) 23, 296
TspDTI ATGAA 4 cut(s) 698, 765, 1205, 1370
TspGWI ACGGA 2 cut(s) 117, 167
TspRI CASTG 3 cut(s) 1104, 1120, 1340
VpaK11BI GGWCC 2 cut(s) 677, 1564
XapI RAATTY 3 cut(s) 316, 473, 1573
XceI RCATGY 2 cut(s) 569, 1508
XcmI CCANNNNNNNNNTGG 3 cut(s) 328, 658, 1575
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 2 cut(s) 218, 1224
Zsp2I ATGCAT 2 cut(s) 346, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.