Rorug03G0249100

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002412
Physical Location & Seq
Forward (+)
23435342 .. 23436501
1160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0249100.1

Sequence Viewer

Length: 315 bp
ATGCTCCGCCAGCGCCTCATCCAAACCCTCCGAACCCGCGGCGGCTCCGCCACCGGCCCGAGCCGCTGGGCCAGTCCCGGCCACGAAGAGCATCCCAAAGGCTACCTCTTCAACCAAACCCCTCCACCTCCAGGCCAGTCCCGAAAATGGGAGGATTGGGAGCTCCCCTGCTACGTCACCAGCTTCCTCACCATCGTCATCCTCGGCGTCGGACTCAATGCCAAGCCCGATCTCACCATCGAGACCTGGGCCCACCAGAAGGCCCTCGAGCGCCTCGAGATCGAGCAGGCCGGCCTTCCGGCTGAATCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

104

Amino Acids

11.69

Weight (kDa)

6.29

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 39
AciI CCGC 6 cut(s) 7, 37, 39, 42, 48, 64
AcoI YGGCCR 1 cut(s) 79
AcyI GRCGYC 1 cut(s) 207
AfiI CCNNNNNNNGG 4 cut(s) 131, 147, 148, 259
AgsI TTSAA 1 cut(s) 112
AjnI CCWGG 2 cut(s) 130, 245
AluBI AGCT 2 cut(s) 163, 183
AluI AGCT 2 cut(s) 163, 183
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 236
Ama87I CYCGRG 3 cut(s) 58, 266, 275
AoxI GGCC 8 cut(s) 55, 69, 79, 133, 249, 261, 288, 292
ApaI GGGCCC 1 cut(s) 253
AspLEI GCGC 2 cut(s) 15, 273
AspS9I GGNCC 5 cut(s) 56, 69, 249, 250, 262
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 3 cut(s) 169, 181, 226
AvaI CYCGRG 3 cut(s) 58, 266, 275
BaeGI GKGCMC 1 cut(s) 253
BanII GRGCYC 2 cut(s) 165, 253
Bbv12I GWGCWC 1 cut(s) 165
BccI CCATC 2 cut(s) 200, 245
BciT130I CCWGG 2 cut(s) 132, 247
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 1 cut(s) 236
BfoI RGCGCY 2 cut(s) 16, 274
BisI GCNGC 3 cut(s) 40, 43, 64
BlsI GCNGC 3 cut(s) 41, 44, 65
Bme1390I CCNGG 3 cut(s) 78, 132, 247
BmeT110I CYCGRG 3 cut(s) 58, 266, 275
BmgT120I GGNCC 5 cut(s) 56, 69, 249, 250, 262
BmiI GGNNCC 2 cut(s) 46, 251
BmrFI CCNGG 3 cut(s) 78, 132, 247
BmsI GCATC 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 3 cut(s) 37, 202, 246
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 147, 148, 259
Bse118I RCCGGY 2 cut(s) 53, 290
Bse1I ACTGG 2 cut(s) 72, 136
BseBI CCWGG 2 cut(s) 132, 247
BseDI CCNNGG 3 cut(s) 37, 202, 246
BseGI GGATG 3 cut(s) 18, 91, 198
BseLI CCNNNNNNNGG 4 cut(s) 131, 147, 148, 259
BseNI ACTGG 2 cut(s) 72, 136
BseSI GKGCMC 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 8 cut(s) 57, 71, 81, 135, 251, 263, 290, 294
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 3 cut(s) 58, 266, 275
BsiSI CCGG 4 cut(s) 54, 78, 291, 299
BslFI GGGAC 2 cut(s) 60, 124
BslI CCNNNNNNNGG 4 cut(s) 131, 147, 148, 259
BsmAI GTCTC 1 cut(s) 236
BsmFI GGGAC 2 cut(s) 60, 124
BsnI GGCC 8 cut(s) 57, 71, 81, 135, 251, 263, 290, 294
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 3 cut(s) 58, 266, 275
Bsp120I GGGCCC 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 165, 253
Bsp143I GATC 2 cut(s) 229, 279
BspACI CCGC 6 cut(s) 7, 37, 39, 42, 48, 64
BspANI GGCC 8 cut(s) 57, 71, 81, 135, 251, 263, 290, 294
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 46, 251
BspQI GCTCTTC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 236
BsrFI RCCGGY 2 cut(s) 53, 290
BsrI ACTGG 2 cut(s) 72, 136
BssAI RCCGGY 2 cut(s) 53, 290
BssECI CCNNGG 3 cut(s) 37, 202, 246
BssMI GATC 2 cut(s) 229, 279
BssNI GRCGYC 1 cut(s) 207
Bst2UI CCWGG 2 cut(s) 132, 247
Bst6I CTCTTC 2 cut(s) 81, 113
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 3 cut(s) 11, 288, 292
BstDSI CCRYGG 1 cut(s) 37
BstF5I GGATG 3 cut(s) 18, 91, 198
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 2 cut(s) 16, 274
BstHHI GCGC 2 cut(s) 15, 273
BstKTI GATC 2 cut(s) 232, 282
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 2 cut(s) 229, 279
BstMWI GCNNNNNNNGC 2 cut(s) 10, 63
BstNI CCWGG 2 cut(s) 132, 247
BstSCI CCNGG 3 cut(s) 76, 130, 245
BstSLI GKGCMC 1 cut(s) 253
BstUI CGCG 1 cut(s) 39
BsuRI GGCC 8 cut(s) 57, 71, 81, 135, 251, 263, 290, 294
BtgI CCRYGG 1 cut(s) 37
BtsCI GGATG 3 cut(s) 18, 91, 198
Cac8I GCNNGC 3 cut(s) 11, 288, 292
CfoI GCGC 2 cut(s) 15, 273
Cfr10I RCCGGY 2 cut(s) 53, 290
Cfr13I GGNCC 5 cut(s) 56, 69, 249, 250, 262
Cfr42I CCGCGG 1 cut(s) 40
CseI GACGC 1 cut(s) 196
DpnI GATC 2 cut(s) 231, 281
DpnII GATC 2 cut(s) 229, 279
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 2 cut(s) 81, 113
EarI CTCTTC 2 cut(s) 81, 113
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 163
Eco24I GRGCYC 2 cut(s) 165, 253
Eco31I GGTCTC 1 cut(s) 236
Eco53kI GAGCTC 1 cut(s) 163
Eco88I CYCGRG 3 cut(s) 58, 266, 275
EcoICRI GAGCTC 1 cut(s) 163
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 130, 245
EcoT38I GRGCYC 2 cut(s) 165, 253
FaqI GGGAC 2 cut(s) 60, 124
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 3 cut(s) 40, 43, 64
FokI GGATG 3 cut(s) 5, 78, 185
FriOI GRGCYC 2 cut(s) 165, 253
FseI GGCCGGCC 1 cut(s) 294
Fsp4HI GCNGC 3 cut(s) 40, 43, 64
GlaI GCGC 2 cut(s) 14, 272
GluI GCNGC 3 cut(s) 40, 43, 64
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 1 cut(s) 114
HaeII RGCGCY 2 cut(s) 16, 274
HaeIII GGCC 8 cut(s) 57, 71, 81, 135, 251, 263, 290, 294
HapII CCGG 4 cut(s) 54, 78, 291, 299
HgaI GACGC 1 cut(s) 196
HhaI GCGC 2 cut(s) 15, 273
Hin1I GRCGYC 1 cut(s) 207
Hin6I GCGC 2 cut(s) 13, 271
HinP1I GCGC 2 cut(s) 13, 271
HinfI GANTC 2 cut(s) 213, 305
HpaII CCGG 4 cut(s) 54, 78, 291, 299
HphI GGTGA 3 cut(s) 169, 181, 226
Hpy188I TCNGA 3 cut(s) 32, 212, 310
Hpy188III TCNNGA 3 cut(s) 141, 241, 277
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 2 cut(s) 253, 305
HpyCH4IV ACGT 1 cut(s) 174
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 63
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 207
HspAI GCGC 2 cut(s) 13, 271
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
KspI CCGCGG 1 cut(s) 40
Kzo9I GATC 2 cut(s) 229, 279
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 4 cut(s) 9, 50, 160, 168
LweI GCATC 1 cut(s) 100
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 175
MalI GATC 2 cut(s) 231, 281
MboI GATC 2 cut(s) 229, 279
MboII GAAGA 2 cut(s) 98, 100
MhlI GDGCHC 2 cut(s) 165, 253
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 190
MroNI GCCGGC 1 cut(s) 290
MspA1I CMGCKG 2 cut(s) 39, 66
MspI CCGG 4 cut(s) 54, 78, 291, 299
MspR9I CCNGG 3 cut(s) 78, 132, 247
MvaI CCWGG 2 cut(s) 132, 247
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 2 cut(s) 10, 63
NaeI GCCGGC 1 cut(s) 292
NciI CCSGG 1 cut(s) 78
NdeII GATC 2 cut(s) 229, 279
NgoMIV GCCGGC 1 cut(s) 290
NlaIV GGNNCC 2 cut(s) 46, 251
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 175
PaeR7I CTCGAG 2 cut(s) 266, 275
PciSI GCTCTTC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 292
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 3 cut(s) 41, 44, 65
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
Psp124BI GAGCTC 1 cut(s) 165
Psp6I CCWGG 2 cut(s) 130, 245
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 2 cut(s) 130, 245
PspN4I GGNNCC 2 cut(s) 46, 251
PspOMI GGGCCC 1 cut(s) 249
PspPI GGNCC 5 cut(s) 56, 69, 249, 250, 262
PspXI VCTCGAGB 1 cut(s) 266
RigI GGCCGGCC 1 cut(s) 294
SacI GAGCTC 1 cut(s) 165
SacII CCGCGG 1 cut(s) 40
SapI GCTCTTC 1 cut(s) 81
SatI GCNGC 3 cut(s) 40, 43, 64
Sau3AI GATC 2 cut(s) 229, 279
Sau96I GGNCC 5 cut(s) 56, 69, 249, 250, 262
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 3 cut(s) 78, 132, 247
SduI GDGCHC 2 cut(s) 165, 253
SetI ASST 6 cut(s) 108, 130, 165, 177, 185, 248
SfaNI GCATC 1 cut(s) 100
Sfr274I CTCGAG 2 cut(s) 266, 275
Sfr303I CCGCGG 1 cut(s) 40
SgrBI CCGCGG 1 cut(s) 40
SlaI CTCGAG 2 cut(s) 266, 275
SmlI CTYRAG 2 cut(s) 266, 275
SmoI CTYRAG 2 cut(s) 266, 275
SsiI CCGC 6 cut(s) 7, 37, 39, 42, 48, 64
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 3 cut(s) 76, 130, 245
TaiI ACGT 1 cut(s) 177
TaqI TCGA 4 cut(s) 240, 267, 276, 282
TauI GCSGC 3 cut(s) 42, 45, 66
TfiI GAWTC 1 cut(s) 305
TseFI GTSAC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 175
XhoI CTCGAG 2 cut(s) 266, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.