Rw3G026330

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002412
Physical Location & Seq
Reverse (-)
36041491 .. 36050693
9203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G026330.1

Sequence Viewer

Length: 720 bp
ATGTATCATCTTGGAACGGTGCTACACCTCCAAGGAAAAGAAAAGGACTCTGAGGCCCTCGTTCAAGATTCCATTCGAATACTTGAGGATGGTGGTCAAGGAGAGTCGGTTATGTGCTTAAAGAGAATGCGATATCTTGCTCAGATATATCTGAAATCCAATCGATTTGAGGAGGCCGAGAAGGTACAAAGAAAGATCCTGCATATAATGGAAATAACAAAGGGATGGAATGCATTGGATACAGCAATAACAGCTGAAGGGCTCGCACTGATCCTCCAATCCACTGGGAGCTTAAAGGAAGCTGAAGAGCTTCTTGAAAGATGTCTTGATGCCCGAAAAGCCTTACTTCCTCAAGATCACGTTCAGATTGGTGTGAACTTGCTTCATATTGCTAGAGTGGCAATGCTCAATTCCGACCAACTGAAGAATATTGACTATCCAAAAGCAATTTCTGAGCTTGATAAGGCCAAAGAAGTTTTACATGAGTCCATGAGGATAGCACAATGTTCCTTGGATAAATTGATGAAGCAAAATAAGAAGATTCGTAGTAATGGAGCACCAGGTGAAATTGGAAAGAATGGTCAGGTGCCATTAGTCATATTGTTGCAAGCTTCTGATGCTCTTGGCCTTTTGGAGATCACAAAGAAACAATTACAAGGGTCACAGAGTTTGTTCTTGGGGTGGCATAGGAGATCTGTGAAGCTGACTAAAGTTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

239

Amino Acids

26.8

Weight (kDa)

8.86

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_MalT PF17874 3 - 131 1.7e-06 MalT-like TPR region
TPR_12 PF13424 45 - 113 8.3e-10 Tetratricopeptide repeat
TPR_10 PF13374 81 - 120 4.8e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 586
AclWI GGATC 2 cut(s) 190, 265
AcuI CTGAAG 3 cut(s) 276, 324, 443
AdeI CACNNNGTG 1 cut(s) 563
AfaI GTAC 1 cut(s) 186
AgsI TTSAA 3 cut(s) 65, 317, 716
AjnI CCWGG 1 cut(s) 559
AjuI GAANNNNNNNTTGG 2 cut(s) 433, 465
AluBI AGCT 7 cut(s) 254, 291, 302, 310, 457, 611, 703
AluI AGCT 7 cut(s) 254, 291, 302, 310, 457, 611, 703
Alw21I GWGCWC 1 cut(s) 559
AlwI GGATC 2 cut(s) 190, 265
AoxI GGCC 4 cut(s) 54, 174, 465, 625
Asp700I GAANNNNTTC 1 cut(s) 309
AspS9I GGNCC 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 575
AsuII TTCGAA 1 cut(s) 76
BanI GGYRCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 559
BccI CCATC 2 cut(s) 83, 219
BciT130I CCWGG 1 cut(s) 561
BciVI GTATCC 1 cut(s) 232
BfaI CTAG 1 cut(s) 393
BfuI GTATCC 1 cut(s) 232
BglII AGATCT 1 cut(s) 692
Bme1390I CCNGG 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 588
BmrFI CCNGG 1 cut(s) 561
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 2 cut(s) 319, 607
BmuI ACTGGG 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 76
BpuEI CTTGAG 2 cut(s) 104, 336
Bsa29I ATCGAT 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 31, 510
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 258, 288
Bse1I ACTGG 1 cut(s) 289
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 1 cut(s) 561
BseCI ATCGAT 1 cut(s) 163
BseDI CCNNGG 2 cut(s) 31, 510
BseGI GGATG 2 cut(s) 94, 230
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 42, 155, 444
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 185
BshFI GGCC 4 cut(s) 56, 176, 467, 627
BshNI GGYRCC 1 cut(s) 586
BshVI ATCGAT 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 559
BsmI GAATGC 2 cut(s) 132, 235
BsnI GGCC 4 cut(s) 56, 176, 467, 627
Bsp119I TTCGAA 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 264, 559
Bsp143I GATC 5 cut(s) 195, 270, 355, 636, 692
BspANI GGCC 4 cut(s) 56, 176, 467, 627
BspCNI CTCAG 3 cut(s) 43, 154, 445
BspDI ATCGAT 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 588
BspPI GGATC 2 cut(s) 190, 265
BspQI GCTCTTC 1 cut(s) 300
BspT104I TTCGAA 1 cut(s) 76
BspT107I GGYRCC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 2 cut(s) 31, 510
BssMI GATC 5 cut(s) 195, 270, 355, 636, 692
BssT1I CCWWGG 2 cut(s) 31, 510
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 300
BstBI TTCGAA 1 cut(s) 76
BstC8I GCNNGC 2 cut(s) 264, 609
BstDEI CTNAG 3 cut(s) 51, 141, 453
BstF5I GGATG 2 cut(s) 94, 230
BstKTI GATC 5 cut(s) 198, 273, 358, 639, 695
BstMBI GATC 5 cut(s) 195, 270, 355, 636, 692
BstMWI GCNNNNNNNGC 4 cut(s) 251, 338, 398, 617
BstNI CCWGG 1 cut(s) 561
BstSCI CCNGG 1 cut(s) 559
BstX2I RGATCY 2 cut(s) 195, 692
BstXI CCANNNNNNTGG 1 cut(s) 284
BstYI RGATCY 2 cut(s) 195, 692
Bsu15I ATCGAT 1 cut(s) 163
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 4 cut(s) 56, 176, 467, 627
BsuTUI ATCGAT 1 cut(s) 163
BtsCI GGATG 2 cut(s) 94, 230
BtsIMutI CAGTG 2 cut(s) 266, 282
Cac8I GCNNGC 2 cut(s) 264, 609
Cfr13I GGNCC 1 cut(s) 55
ClaI ATCGAT 1 cut(s) 163
CsiI ACCWGGT 1 cut(s) 559
Csp6I GTAC 1 cut(s) 185
CviAII CATG 2 cut(s) 482, 490
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 3 cut(s) 51, 141, 453
DpnI GATC 5 cut(s) 197, 272, 357, 638, 694
DpnII GATC 5 cut(s) 195, 270, 355, 636, 692
DraIII CACNNNGTG 1 cut(s) 563
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco130I CCWWGG 2 cut(s) 31, 510
Eco24I GRGCYC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 134
Eco57I CTGAAG 3 cut(s) 276, 324, 443
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 559
EcoRV GATATC 1 cut(s) 134
EcoT14I CCWWGG 2 cut(s) 31, 510
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 2 cut(s) 31, 510
FaeI CATG 2 cut(s) 485, 493
FaiI YATR 9 cut(s) 113, 148, 204, 206, 387, 483, 491, 599, 687
FalI AAGNNNNNCTT 4 cut(s) 297, 329, 330, 362
FatI CATG 2 cut(s) 481, 489
FokI GGATG 2 cut(s) 101, 237
FriOI GRGCYC 1 cut(s) 264
FspBI CTAG 1 cut(s) 393
HaeIII GGCC 4 cut(s) 56, 176, 467, 627
Hin1II CATG 2 cut(s) 485, 493
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 5 cut(s) 47, 68, 104, 485, 541
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 7 cut(s) 52, 144, 153, 366, 415, 454, 616
Hpy188III TCNNGA 4 cut(s) 65, 314, 326, 353
Hpy8I GTNNAC 1 cut(s) 376
HpyAV CCTTC 2 cut(s) 175, 251
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 3 cut(s) 202, 233, 607
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 338, 398, 617
HpyF3I CTNAG 3 cut(s) 51, 141, 453
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 2 cut(s) 485, 493
Kzo9I GATC 5 cut(s) 195, 270, 355, 636, 692
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 2 cut(s) 288, 554
LpnPI CCDG 5 cut(s) 212, 270, 546, 569, 573
LweI GCATC 2 cut(s) 319, 607
MabI ACCWGGT 1 cut(s) 559
MaeI CTAG 1 cut(s) 393
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 660
MalI GATC 5 cut(s) 197, 272, 357, 638, 694
MboI GATC 5 cut(s) 195, 270, 355, 636, 692
MboII GAAGA 3 cut(s) 317, 436, 550
MflI RGATCY 2 cut(s) 195, 692
MhlI GDGCHC 2 cut(s) 264, 559
MluCI AATT 5 cut(s) 409, 447, 518, 567, 650
MlyI GAGTC 3 cut(s) 41, 113, 494
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 9 cut(s) 38, 46, 68, 79, 163, 166, 284, 360, 486
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 2 cut(s) 119, 293
MspA1I CMGCKG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 561
Mva1269I GAATGC 2 cut(s) 132, 235
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 4 cut(s) 251, 338, 398, 617
NdeII GATC 5 cut(s) 195, 270, 355, 636, 692
NlaIII CATG 2 cut(s) 485, 493
NlaIV GGNNCC 1 cut(s) 588
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 660
NsiI ATGCAT 1 cut(s) 235
NspV TTCGAA 1 cut(s) 76
PciSI GCTCTTC 1 cut(s) 300
PctI GAATGC 2 cut(s) 132, 235
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 2 cut(s) 68, 541
PleI GAGTC 3 cut(s) 41, 112, 493
PpsI GAGTC 3 cut(s) 41, 112, 493
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 1 cut(s) 588
PspPI GGNCC 1 cut(s) 55
PsuI RGATCY 2 cut(s) 195, 692
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 2 cut(s) 119, 293
Sau3AI GATC 5 cut(s) 195, 270, 355, 636, 692
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 3 cut(s) 41, 113, 494
ScrFI CCNGG 1 cut(s) 561
SduI GDGCHC 2 cut(s) 264, 559
SexAI ACCWGGT 1 cut(s) 559
SfaNI GCATC 2 cut(s) 319, 607
SfuI TTCGAA 1 cut(s) 76
SmlI CTYRAG 2 cut(s) 83, 351
SmoI CTYRAG 2 cut(s) 83, 351
Sse9I AATT 5 cut(s) 409, 447, 518, 567, 650
SspI AATATT 1 cut(s) 430
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 559
StyI CCWWGG 2 cut(s) 31, 510
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 76, 163
TasI AATT 5 cut(s) 409, 447, 518, 567, 650
TfiI GAWTC 2 cut(s) 68, 541
Tru1I TTAA 2 cut(s) 119, 293
Tru9I TTAA 2 cut(s) 119, 293
TscAI CASTG 2 cut(s) 273, 289
TseFI GTSAC 1 cut(s) 660
Tsp45I GTSAC 1 cut(s) 660
TspDTI ATGAA 2 cut(s) 374, 539
TspRI CASTG 2 cut(s) 273, 289
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 1 cut(s) 393
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.