RLG00000022845

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002412
Physical Location & Seq
Forward (+)
13191606 .. 13204512
12907 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022845

Sequence Viewer

Length: 1683 bp
ATGTCTTGTGTGCGGCGAGCGGTAGCTAAGGTGGTGAAGGAGAAGAGGCATATAGTTAACCGTTTGCTCTCTCCTTCTTCTTCAACTTCCGGAACCAGAACCGGAACCATCCCTACTGCTCCGTTCTACTCTGGCGGTACTGGCGGTTACAAGTTTTCTTATCAGCATTGGAGGATCGACCCTCGGCTCTGGTTTTTTCTATCTGGAAATGCAGCAATTATTCTTGCCATAAAGTCCAACACCGTCTTGGCAAAAGATGCATCACTTGATAATGAGGCACAGGGGATGGAAGGGGATGACACAATGCAAATACGCAGAGTTGAGGATGCTTCTGTCAAATCTAACATACATACCTCTAAGTGGAGAGTTTTTACAGATAAAGGGAGGGAATTCTTCCTTAAGGGAAAAGTGGAGGAAGCTGAAAAGCTCTTTCTCGCGGCTTTACAGGAAGCTAAAGAAGGTTTTGGGAAGAGGGATCCACATGTTGCATCTGCAAGCAATAATTTAGCAGAGCTCTATAGAGTTAATAAGCTATATAATAAGGCAGAGCCCTTGTATTTGGAGGCCATCAGCATACTGGAGGAGTCATTTGGTCCTGAAGATATACGAGTTGGGTCTGCCTTTCATAACCTGGGTCAGTTCTATATTGTACAGCGGAAGCTGGATGAAGCCCGTGTTTGCTATGAGCGTGCTTTGAAGATTAAACGCCATGTTCTGGGTCTTAACCATACAGGTTATGCAGACACCATGTATCATCTTGGAACGGTGCTACACCTCCAAGGAAGAGAAAAGGAATCTGAGGCCCTCATTCAAGATTCCATTCGAATACTTGAGGATGGTGGTCAAGGAGAGTCGGTTATGTGCTTAAAGAGAATGCGATATCTTGCTCAGATATATCTGAAATCCAATCGATTTGAGGAGGCCGAGAAGGTACAAAGAAAGATCCTGCATATAATGGAAATAACAAAGGGATGGAATGCATTGGATACAGCAATAACAGCTGAAGGGCTCGCACTGATCCTCCAATCCACTGGGAGCTTAAAGGAAGCTGAAGAGCTTCTTGAAAGATGTCTTGATGCTCGAAAAGCCTTACTTCCTCAAGATCACATTCAGATTGGTGTGAACTTGCTTCATATTGCTAGAGTGGCAATGCTCAATTCCGACCAACTGAAGAATATTGACTATCCAAAAGCAATTTCTGAGCTTGATAAGGCCGAAGAAGTTTTACATGAGTCCATGAGGATAGCACAATGTTCCTTGGATAAATTGGTGAAACAAAATAAGAAGATTCGTAGTAATGGAGCACCAGGTGAAATTGGAAAGAATGGTCAGGTGCCATTAGTCATATTGTTGCAAGCTTCTGATGCTCTTGGCCTTTTGGAGATCACAAAGAAACAATTACAAGGGTCAAAGCAGTCTGAGCATGCACCTAATATCGAAGCTGAGAATGCACTATTTAAATGTATATCTGCTTACAAAGAGTTCGTAACTGAGAGATCAATCCCTGAGTCTCCTGAAACAAAGGCCGAGTATCTTAAATGCTTGAAGCATCTTTTGAGCTTGAATAGTGATAGCACTACAGAAAGAACACAGCACTCTACTGGAGTCAATTTGCAAGATCTGAGAGACGAAATTAAGCGTCTTGAAGTTGAACTTTCTCCTCATAGGAAAAGAAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

561

Amino Acids

63.03

Weight (kDa)

8.77

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 133 - 195 6.8e-08 Tetratricopeptide repeat
TPR_NPHP3 PF24885 135 - 237 1.1e-11 Nephrocystin-3 TPR domain
TPR_12 PF13424 162 - 213 2.3e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 204 - 320 2.2e-08 Nephrocystin-3 TPR domain
TPR_12 PF13424 205 - 278 4.3e-14 Tetratricopeptide repeat
TPR_10 PF13374 206 - 243 3.7e-07 Tetratricopeptide repeat
TPR_MalT PF17874 250 - 380 4.1e-06 MalT-like TPR region
TPR_12 PF13424 294 - 362 3.8e-09 Tetratricopeptide repeat
TPR_10 PF13374 330 - 370 1.3e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1333
AccB7I CCANNNNNTGG 1 cut(s) 715
AccBSI CCGCTC 1 cut(s) 20
AccII CGCG 1 cut(s) 437
AccIII TCCGGA 1 cut(s) 89
AciI CCGC 6 cut(s) 13, 20, 135, 144, 437, 655
AclWI GGATC 5 cut(s) 182, 470, 483, 937, 1012
AcsI RAATTY 2 cut(s) 389, 1677
AcuI CTGAAG 4 cut(s) 618, 1023, 1071, 1190
AdeI CACNNNGTG 1 cut(s) 1310
AfaI GTAC 3 cut(s) 139, 651, 933
AfiI CCNNNNNNNGG 2 cut(s) 360, 715
AflII CTTAAG 1 cut(s) 398
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 8 cut(s) 84, 697, 812, 1064, 1546, 1564, 1646, 1652
AjnI CCWGG 2 cut(s) 630, 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 1180, 1212
Alw21I GWGCWC 2 cut(s) 516, 1306
Alw26I GTCTC 2 cut(s) 1515, 1620
AlwI GGATC 5 cut(s) 182, 470, 483, 937, 1012
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 6 cut(s) 564, 801, 921, 1212, 1372, 1524
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 2 cut(s) 389, 1677
Asp700I GAANNNNTTC 1 cut(s) 1056
AspS9I GGNCC 2 cut(s) 593, 802
AsuHPI GGTGA 3 cut(s) 46, 1282, 1322
AsuII TTCGAA 1 cut(s) 823
AvaII GGWCC 1 cut(s) 593
BamHI GGATCC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 1333
BanII GRGCYC 3 cut(s) 516, 552, 1011
BarI GAAGNNNNNNTAC 2 cut(s) 1209, 1241
Bbv12I GWGCWC 2 cut(s) 516, 1306
BbvI GCAGC 1 cut(s) 224
BccI CCATC 5 cut(s) 116, 280, 575, 830, 966
BciT130I CCWGG 2 cut(s) 632, 1308
BciVI GTATCC 1 cut(s) 979
BcoDI GTCTC 2 cut(s) 1515, 1620
BfaI CTAG 1 cut(s) 1140
BfmI CTRYAG 2 cut(s) 517, 1578
BfrI CTTAAG 1 cut(s) 398
BfuI GTATCC 1 cut(s) 979
BglII AGATCT 1 cut(s) 1618
BisI GCNGC 3 cut(s) 14, 213, 438
BlsI GCNGC 3 cut(s) 15, 214, 439
Bme1390I CCNGG 2 cut(s) 632, 1308
Bme18I GGWCC 1 cut(s) 593
BmgT120I GGNCC 2 cut(s) 593, 802
BmiI GGNNCC 4 cut(s) 94, 106, 477, 1335
BmrFI CCNGG 2 cut(s) 632, 1308
BmrI ACTGGG 1 cut(s) 1041
BmsI GCATC 7 cut(s) 247, 269, 316, 497, 1066, 1354, 1558
BmuI ACTGGG 1 cut(s) 1041
BpmI CTGGAG 2 cut(s) 599, 1623
Bpu10I CCTNAGC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 823
BpuEI CTTGAG 2 cut(s) 851, 1083
Bsa29I ATCGAT 1 cut(s) 910
BsaJI CCNNGG 4 cut(s) 182, 631, 778, 1257
BsaWI WCCGGW 2 cut(s) 89, 101
BsaXI ACNNNNNCTCC 4 cut(s) 840, 870, 1005, 1035
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 715
Bse1I ACTGG 4 cut(s) 145, 582, 1036, 1606
Bse3DI GCAATG 1 cut(s) 1155
BseAI TCCGGA 1 cut(s) 89
BseBI CCWGG 2 cut(s) 632, 1308
BseCI ATCGAT 1 cut(s) 910
BseDI CCNNGG 4 cut(s) 182, 631, 778, 1257
BseGI GGATG 7 cut(s) 108, 291, 301, 331, 670, 841, 977
BseLI CCNNNNNNNGG 2 cut(s) 360, 715
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 8 cut(s) 789, 902, 1191, 1410, 1434, 1482, 1497, 1613
BseNI ACTGG 4 cut(s) 145, 582, 1036, 1606
BseRI GAGGAG 3 cut(s) 596, 932, 1650
BseXI GCAGC 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 437
BshFI GGCC 6 cut(s) 566, 803, 923, 1214, 1374, 1526
BshNI GGYRCC 1 cut(s) 1333
BshVI ATCGAT 1 cut(s) 910
BsiHKAI GWGCWC 2 cut(s) 516, 1306
BsiSI CCGG 2 cut(s) 90, 102
BslI CCNNNNNNNGG 2 cut(s) 360, 715
BsmAI GTCTC 2 cut(s) 1515, 1620
BsmBI CGTCTC 1 cut(s) 1620
BsmI GAATGC 3 cut(s) 879, 982, 1453
BsnI GGCC 6 cut(s) 566, 803, 923, 1214, 1374, 1526
Bsp119I TTCGAA 1 cut(s) 823
Bsp1286I GDGCHC 4 cut(s) 516, 552, 1011, 1306
Bsp13I TCCGGA 1 cut(s) 89
Bsp1407I TGTACA 1 cut(s) 649
Bsp143I GATC 8 cut(s) 174, 475, 942, 1017, 1102, 1383, 1496, 1618
BspACI CCGC 6 cut(s) 13, 20, 135, 144, 437, 655
BspANI GGCC 6 cut(s) 566, 803, 923, 1214, 1374, 1526
BspCNI CTCAG 8 cut(s) 790, 901, 1192, 1411, 1435, 1483, 1498, 1614
BspDI ATCGAT 1 cut(s) 910
BspEI TCCGGA 1 cut(s) 89
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 4 cut(s) 94, 106, 477, 1335
BspPI GGATC 5 cut(s) 182, 470, 483, 937, 1012
BspQI GCTCTTC 1 cut(s) 1047
BspT104I TTCGAA 1 cut(s) 823
BspT107I GGYRCC 1 cut(s) 1333
BspTI CTTAAG 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 1155
BsrGI TGTACA 1 cut(s) 649
BsrI ACTGG 4 cut(s) 145, 582, 1036, 1606
BssECI CCNNGG 4 cut(s) 182, 631, 778, 1257
BssMI GATC 8 cut(s) 174, 475, 942, 1017, 1102, 1383, 1496, 1618
BssT1I CCWWGG 2 cut(s) 778, 1257
Bst2UI CCWGG 2 cut(s) 632, 1308
Bst4CI ACNGT 3 cut(s) 62, 244, 766
Bst6I CTCTTC 4 cut(s) 38, 464, 778, 1047
BstAFI CTTAAG 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 257
BstAUI TGTACA 1 cut(s) 649
BstBI TTCGAA 1 cut(s) 823
BstC8I GCNNGC 6 cut(s) 18, 496, 690, 1011, 1356, 1425
BstF5I GGATG 7 cut(s) 108, 291, 301, 331, 670, 841, 977
BstFNI CGCG 1 cut(s) 437
BstKTI GATC 8 cut(s) 177, 478, 945, 1020, 1105, 1386, 1499, 1621
BstMAI GTCTC 2 cut(s) 1515, 1620
BstMBI GATC 8 cut(s) 174, 475, 942, 1017, 1102, 1383, 1496, 1618
BstMWI GCNNNNNNNGC 8 cut(s) 141, 257, 998, 1085, 1145, 1364, 1420, 1448
BstNI CCWGG 2 cut(s) 632, 1308
BstNSI RCATGY 2 cut(s) 485, 1427
BstSCI CCNGG 2 cut(s) 630, 1306
BstSFI CTRYAG 2 cut(s) 517, 1578
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 1 cut(s) 224
BstX2I RGATCY 3 cut(s) 475, 942, 1618
BstXI CCANNNNNNTGG 1 cut(s) 1031
BstYI RGATCY 3 cut(s) 475, 942, 1618
Bsu15I ATCGAT 1 cut(s) 910
BsuI GTATCC 1 cut(s) 979
BsuRI GGCC 6 cut(s) 566, 803, 923, 1214, 1374, 1526
BsuTUI ATCGAT 1 cut(s) 910
BtsCI GGATG 7 cut(s) 108, 291, 301, 331, 670, 841, 977
BtsIMutI CAGTG 2 cut(s) 1013, 1029
Cac8I GCNNGC 6 cut(s) 18, 496, 690, 1011, 1356, 1425
Cfr13I GGNCC 2 cut(s) 593, 802
ClaI ATCGAT 1 cut(s) 910
CseI GACGC 1 cut(s) 1628
CsiI ACCWGGT 1 cut(s) 1306
Csp6I GTAC 3 cut(s) 138, 650, 932
CviAII CATG 6 cut(s) 482, 710, 748, 1229, 1237, 1424
CviQI GTAC 3 cut(s) 138, 650, 932
DpnI GATC 8 cut(s) 176, 477, 944, 1019, 1104, 1385, 1498, 1620
DpnII GATC 8 cut(s) 174, 475, 942, 1017, 1102, 1383, 1496, 1618
DraI TTTAAA 1 cut(s) 1459
DraIII CACNNNGTG 1 cut(s) 1310
Eam1104I CTCTTC 4 cut(s) 38, 464, 778, 1047
EarI CTCTTC 4 cut(s) 38, 464, 778, 1047
Ecl136II GAGCTC 1 cut(s) 514
Eco130I CCWWGG 2 cut(s) 778, 1257
Eco24I GRGCYC 3 cut(s) 516, 552, 1011
Eco32I GATATC 1 cut(s) 881
Eco47I GGWCC 1 cut(s) 593
Eco53kI GAGCTC 1 cut(s) 514
Eco57I CTGAAG 4 cut(s) 618, 1023, 1071, 1190
EcoICRI GAGCTC 1 cut(s) 514
EcoO109I RGGNCCY 1 cut(s) 802
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 2 cut(s) 630, 1306
EcoRV GATATC 1 cut(s) 881
EcoT14I CCWWGG 2 cut(s) 778, 1257
EcoT22I ATGCAT 2 cut(s) 262, 982
EcoT38I GRGCYC 3 cut(s) 516, 552, 1011
ErhI CCWWGG 2 cut(s) 778, 1257
Esp3I CGTCTC 1 cut(s) 1620
FaeI CATG 6 cut(s) 485, 713, 751, 1232, 1240, 1427
FalI AAGNNNNNCTT 6 cut(s) 1044, 1076, 1077, 1109, 1638, 1670
FatI CATG 6 cut(s) 481, 709, 747, 1228, 1236, 1423
Fnu4HI GCNGC 3 cut(s) 14, 213, 438
FokI GGATG 7 cut(s) 95, 298, 308, 338, 677, 848, 984
FriOI GRGCYC 3 cut(s) 516, 552, 1011
Fsp4HI GCNGC 3 cut(s) 14, 213, 438
FspBI CTAG 1 cut(s) 1140
GluI GCNGC 3 cut(s) 14, 213, 438
GsuI CTGGAG 2 cut(s) 599, 1623
HaeIII GGCC 6 cut(s) 566, 803, 923, 1214, 1374, 1526
HapII CCGG 2 cut(s) 90, 102
HgaI GACGC 1 cut(s) 1628
Hin1II CATG 6 cut(s) 485, 713, 751, 1232, 1240, 1427
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 1 cut(s) 1356
HinfI GANTC 8 cut(s) 584, 794, 815, 851, 1232, 1288, 1508, 1605
HpaI GTTAAC 1 cut(s) 58
HpaII CCGG 2 cut(s) 90, 102
HphI GGTGA 3 cut(s) 46, 1282, 1322
Hpy166II GTNNAC 2 cut(s) 58, 1123
Hpy188I TCNGA 9 cut(s) 799, 891, 900, 1113, 1162, 1201, 1363, 1420, 1623
Hpy188III TCNNGA 9 cut(s) 90, 204, 596, 812, 1061, 1073, 1100, 1514, 1643
Hpy8I GTNNAC 2 cut(s) 58, 1123
HpyAV CCTTC 6 cut(s) 31, 84, 284, 452, 922, 998
HpyCH4III ACNGT 3 cut(s) 62, 244, 766
HpyF10VI GCNNNNNNNGC 8 cut(s) 141, 257, 998, 1085, 1145, 1364, 1420, 1448
Hsp92II CATG 6 cut(s) 485, 713, 751, 1232, 1240, 1427
Kpn2I TCCGGA 1 cut(s) 89
KspAI GTTAAC 1 cut(s) 58
Kzo9I GATC 8 cut(s) 174, 475, 942, 1017, 1102, 1383, 1496, 1618
LguI GCTCTTC 1 cut(s) 1047
LmnI GCTCC 3 cut(s) 124, 1035, 1301
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 7 cut(s) 247, 269, 316, 497, 1066, 1354, 1558
MabI ACCWGGT 1 cut(s) 1306
MaeI CTAG 1 cut(s) 1140
MaeIII GTNAC 2 cut(s) 146, 1486
MalI GATC 8 cut(s) 176, 477, 944, 1019, 1104, 1385, 1498, 1620
MbiI CCGCTC 1 cut(s) 20
MboI GATC 8 cut(s) 174, 475, 942, 1017, 1102, 1383, 1496, 1618
MflI RGATCY 3 cut(s) 475, 942, 1618
MhlI GDGCHC 4 cut(s) 516, 552, 1011, 1306
MlyI GAGTC 5 cut(s) 593, 860, 1241, 1517, 1614
MmeI TCCRAC 2 cut(s) 261, 1185
Mph1103I ATGCAT 2 cut(s) 262, 982
MroI TCCGGA 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 1056
MspA1I CMGCKG 2 cut(s) 655, 1001
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 2 cut(s) 90, 102
MspR9I CCNGG 2 cut(s) 632, 1308
Mva1269I GAATGC 3 cut(s) 879, 982, 1453
MvaI CCWGG 2 cut(s) 632, 1308
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 8 cut(s) 141, 257, 998, 1085, 1145, 1364, 1420, 1448
NdeII GATC 8 cut(s) 174, 475, 942, 1017, 1102, 1383, 1496, 1618
NlaIII CATG 6 cut(s) 485, 713, 751, 1232, 1240, 1427
NlaIV GGNNCC 4 cut(s) 94, 106, 477, 1335
NmeAIII GCCGAG 3 cut(s) 163, 949, 1552
NsiI ATGCAT 2 cut(s) 262, 982
NspI RCATGY 2 cut(s) 485, 1427
NspV TTCGAA 1 cut(s) 823
PaeI GCATGC 1 cut(s) 1427
PciI ACATGT 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 1047
PctI GAATGC 3 cut(s) 879, 982, 1453
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 3 cut(s) 794, 815, 1288
PflMI CCANNNNNTGG 1 cut(s) 715
PkrI GCNGC 3 cut(s) 15, 214, 439
PleI GAGTC 5 cut(s) 592, 859, 1240, 1516, 1613
PpsI GAGTC 5 cut(s) 592, 859, 1240, 1516, 1613
PscI ACATGT 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 516
Psp6I CCWGG 2 cut(s) 630, 1306
PspGI CCWGG 2 cut(s) 630, 1306
PspN4I GGNNCC 4 cut(s) 94, 106, 477, 1335
PspPI GGNCC 2 cut(s) 593, 802
PsrI GAACNNNNNNTAC 2 cut(s) 97, 129
PsuI RGATCY 3 cut(s) 475, 942, 1618
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 3 cut(s) 139, 651, 933
RsaNI GTAC 3 cut(s) 138, 650, 932
SacI GAGCTC 1 cut(s) 516
SapI GCTCTTC 1 cut(s) 1047
SatI GCNGC 3 cut(s) 14, 213, 438
Sau3AI GATC 8 cut(s) 174, 475, 942, 1017, 1102, 1383, 1496, 1618
Sau96I GGNCC 2 cut(s) 593, 802
SchI GAGTC 5 cut(s) 593, 860, 1241, 1517, 1614
ScrFI CCNGG 2 cut(s) 632, 1308
SduI GDGCHC 4 cut(s) 516, 552, 1011, 1306
SexAI ACCWGGT 1 cut(s) 1306
SfaNI GCATC 7 cut(s) 247, 269, 316, 497, 1066, 1354, 1558
SfcI CTRYAG 2 cut(s) 517, 1578
SfuI TTCGAA 1 cut(s) 823
SinI GGWCC 1 cut(s) 593
SmiI ATTTAAAT 1 cut(s) 1459
SmlI CTYRAG 3 cut(s) 398, 830, 1098
SmoI CTYRAG 3 cut(s) 398, 830, 1098
SphI GCATGC 1 cut(s) 1427
SsiI CCGC 6 cut(s) 13, 20, 135, 144, 437, 655
SspI AATATT 1 cut(s) 1177
SspMI CTAG 1 cut(s) 1140
SstI GAGCTC 1 cut(s) 516
StyD4I CCNGG 2 cut(s) 630, 1306
StyI CCWWGG 2 cut(s) 778, 1257
SwaI ATTTAAAT 1 cut(s) 1459
TaaI ACNGT 3 cut(s) 62, 244, 766
TaqI TCGA 5 cut(s) 177, 823, 910, 1081, 1437
TatI WGTACW 1 cut(s) 649
TauI GCSGC 2 cut(s) 16, 440
TfiI GAWTC 3 cut(s) 794, 815, 1288
TscAI CASTG 2 cut(s) 1020, 1036
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 3 cut(s) 614, 681, 1121
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 2 cut(s) 1020, 1036
Van91I CCANNNNNTGG 1 cut(s) 715
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 2 cut(s) 389, 1677
XceI RCATGY 2 cut(s) 485, 1427
XcmI CCANNNNNNNNNTGG 2 cut(s) 244, 574
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 1 cut(s) 1140
Zsp2I ATGCAT 2 cut(s) 262, 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.