pycom17g26710

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002412
Physical Location & Seq
Forward (+)
24999694 .. 25008364
8671 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g26710.12

Sequence Viewer

Length: 1608 bp
ATGTCTCTGCGACAAGCAGCAGCTAACATGATCAAGCGGCCTTTCCGTTTGGCCTCTCAAACTTTCTCTTCAACTTGTGGACGAGCAGCCACTGCTATTCCACTCGCCAGCCGTGCCAAATTTGATCACGGTTCTAAGGATTGTAGTTGGAGGATGAATTACAGCCACAGGGACCCTCGTCTGTGGCTTTTTGTATCCGGCAATGCAGCTATTTTTCTTGGGATACATTCCAATACTGTCTTAGCAAAAGATGAATCAGTTGATCCTGATAATGGCACGGAAGGCGCTGAAGCCGTGGGATTGCGCAAGATTGAGGACGGTTCTGTGATATCAAATATACATACTTCTAAGTGGAGAATTTTTACTGATAAAGGGAGGGATTTTTTCCTTCAGGGAAAGTTGGAGGAAGCTGAAAAGCTTTTCCTCTCAGCTTTACAAGAAGCGAAAGAAGGCTTTGGGGAAAGGGAGCCACATGTTGCATCTGCATGCAACAATCTAGCAGAGCTCTATAGGGTTAATAAGGCATATAATAAAGCAGAGCCTTTGTACTTGGAAGCTATCAGCATACTGGAGGAATCATTTGGTCCTGAAGATATAAGAGTTGGGGTTGCCTTTCACAACCTTGGCCAGTTCTATATTGCCCAACGGAAGCTTGAAGAAGCTCGCACTTGCTATGAGCGTGCTTTGAAGATTAAGGGCCGCGTTTTGGGTCTTAACCATTCAGATTATGCAGATACTATGTATCATCTTGGAACGGTGCTACACCTCCAAGGAAAAGAAAAGGATTCTGAGGCCCTTATTCAGGATTCAATCAGAATACTTGAGGATGGTGGCCTAGGGGAGTCAATGGTGTGTGTCAAGAGAATGCGATCTCTTGCTCAGATGTATACGAAATCCAATCGTTTTGCAGAGGCTGAGAACGTACAAAGAAAGATTCTACATATAATGGAATTAACAAAGGGATGGAATTCAATGGAAACAGTTATAACAGCTGAGGGGCTCGCACTGATCCTACAATCCGCTGGGATCTTAAAGGATGCTGAAGAGCTTCTTGAAAGATGTCTTGATGCTCGGAAAACCTTACTTCCTGAAGATCATATCCAGATTGGTGCAAATATGCTTCACATTGCTAGAGTGGCAATGCTCAATTCCAACCAACTAAAGACGATGGACACTTCTAAAGCAATTGCTGAGCTTGACAAGGCCAGAGATAATTTAAATGAGTCCATAAGGATAGCACGGTGTTCTTTGGATAAATCAATCAAGAAGAATCGAAAGATGAAAAGTGATATTGGAAGGAGTTGTCGTGTGCCGTTGGTCATAATGTTGCAAGCATTTGACGCTCTTAGCCTTGTGGCGATCGCTAAGCAAGAATTACAGGAATCAAAGCAGGATGAGTGTTCACCTAACGTCGAAGCTGAGGATGCACTATTTAGATGCATCTCTGCTTACAAAGAGTTTGTAACTGAGAAGTCAATTGCTGAGTTACCTGAAGTAAAGGCAGAGTATCTTAAATGTTTGAAGCATCTTATAAGCTTAAATGGTGGTAGCAGTACCCAAACGTCACAGCAATCTACAAGAGTCAATTTGCAACGATCTGGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

536

Amino Acids

59.84

Weight (kDa)

8.17

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 986, 1532
Acc16I TGCGCA 1 cut(s) 305
AccI GTMKAC 1 cut(s) 887
AccII CGCG 1 cut(s) 702
AciI CCGC 3 cut(s) 37, 700, 1020
AclWI GGATC 3 cut(s) 257, 1003, 1034
AcoI YGGCCR 1 cut(s) 625
AcsI RAATTY 3 cut(s) 119, 357, 967
AcuI CTGAAG 6 cut(s) 309, 374, 609, 1062, 1110, 1512
AfaI GTAC 3 cut(s) 548, 924, 1555
AfiI CCNNNNNNNGG 3 cut(s) 272, 706, 802
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 7 cut(s) 72, 656, 688, 810, 972, 1055, 1522
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 3 cut(s) 257, 1003, 1034
AlwNI CAGNNNCTG 2 cut(s) 92, 914
AoxI GGCC 7 cut(s) 38, 51, 625, 697, 792, 832, 1203
ApeKI GCWGC 4 cut(s) 17, 20, 86, 206
ApoI RAATTY 3 cut(s) 119, 357, 967
AsiSI GCGATCGC 1 cut(s) 1362
Asp700I GAANNNNTTC 1 cut(s) 1047
AspA2I CCTAGG 1 cut(s) 835
AspLEI GCGC 2 cut(s) 287, 306
AspS9I GGNCC 4 cut(s) 172, 584, 697, 793
AsuHPI GGTGA 1 cut(s) 1395
AvaII GGWCC 2 cut(s) 172, 584
AvrII CCTAGG 1 cut(s) 835
BalI TGGCCA 1 cut(s) 627
BanII GRGCYC 2 cut(s) 507, 1002
Bbv12I GWGCWC 1 cut(s) 507
BbvCI CCTCAGC 2 cut(s) 993, 1419
BbvI GCAGC 4 cut(s) 29, 32, 98, 218
BccI CCATC 3 cut(s) 821, 957, 1162
BceAI ACGGC 3 cut(s) 96, 278, 1297
BciVI GTATCC 2 cut(s) 205, 216
BclI TGATCA 2 cut(s) 30, 124
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 3 cut(s) 497, 836, 1131
BfmI CTRYAG 1 cut(s) 508
BfoI RGCGCY 1 cut(s) 288
BfuI GTATCC 2 cut(s) 205, 216
BisI GCNGC 6 cut(s) 18, 21, 38, 87, 207, 700
BlnI CCTAGG 1 cut(s) 835
BlpI GCTNAGC 2 cut(s) 1191, 1365
BlsI GCNGC 6 cut(s) 19, 22, 39, 88, 208, 701
Bme18I GGWCC 2 cut(s) 172, 584
BmgT120I GGNCC 4 cut(s) 172, 584, 697, 793
BmiI GGNNCC 3 cut(s) 173, 174, 468
BmsI GCATC 7 cut(s) 488, 1027, 1057, 1414, 1427, 1449, 1534
BoxI GACNNNNGTC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 590
Bpu10I CCTNAGC 2 cut(s) 993, 1419
Bpu1102I GCTNAGC 2 cut(s) 1191, 1365
BpuEI CTTGAG 1 cut(s) 842
BsaJI CCNNGG 4 cut(s) 294, 622, 769, 835
BsaXI ACNNNNNCTCC 2 cut(s) 1291, 1321
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 706, 802
Bse1I ACTGG 2 cut(s) 573, 628
Bse3DI GCAATG 3 cut(s) 208, 1125, 1146
BseDI CCNNGG 4 cut(s) 294, 622, 769, 835
BseGI GGATG 6 cut(s) 159, 832, 968, 1042, 1399, 1429
BseLI CCNNNNNNNGG 3 cut(s) 272, 706, 802
BseMI GCAATG 3 cut(s) 208, 1125, 1146
BseMII CTCAG 9 cut(s) 441, 780, 893, 906, 984, 1182, 1410, 1458, 1473
BseNI ACTGG 2 cut(s) 573, 628
BseXI GCAGC 4 cut(s) 29, 32, 98, 218
BseYI CCCAGC 1 cut(s) 1022
Bsh1236I CGCG 1 cut(s) 702
Bsh1285I CGRYCG 1 cut(s) 1362
BshFI GGCC 7 cut(s) 40, 53, 627, 699, 794, 834, 1205
BsiEI CGRYCG 1 cut(s) 1362
BsiHKAI GWGCWC 1 cut(s) 507
BsiSI CCGG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 3 cut(s) 272, 706, 802
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 870
BsnI GGCC 7 cut(s) 40, 53, 627, 699, 794, 834, 1205
Bsp1286I GDGCHC 2 cut(s) 507, 1002
Bsp143I GATC 9 cut(s) 30, 124, 262, 869, 1008, 1026, 1093, 1359, 1595
Bsp1720I GCTNAGC 2 cut(s) 1191, 1365
BspACI CCGC 3 cut(s) 37, 700, 1020
BspANI GGCC 7 cut(s) 40, 53, 627, 699, 794, 834, 1205
BspCNI CTCAG 9 cut(s) 440, 781, 892, 907, 985, 1183, 1411, 1459, 1474
BspFNI CGCG 1 cut(s) 702
BspLI GGNNCC 3 cut(s) 173, 174, 468
BspPI GGATC 3 cut(s) 257, 1003, 1034
BspQI GCTCTTC 1 cut(s) 1038
BsrDI GCAATG 3 cut(s) 208, 1125, 1146
BsrI ACTGG 2 cut(s) 573, 628
BssECI CCNNGG 4 cut(s) 294, 622, 769, 835
BssMI GATC 9 cut(s) 30, 124, 262, 869, 1008, 1026, 1093, 1359, 1595
BssNAI GTATAC 1 cut(s) 888
BssT1I CCWWGG 3 cut(s) 622, 769, 835
Bst1107I GTATAC 1 cut(s) 888
Bst4CI ACNGT 6 cut(s) 131, 238, 320, 757, 982, 1242
Bst6I CTCTTC 2 cut(s) 73, 1038
BstAPI GCANNNNNTGC 1 cut(s) 92
BstC8I GCNNGC 6 cut(s) 109, 487, 664, 681, 1002, 1332
BstDSI CCRYGG 1 cut(s) 294
BstENI CCTNNNNNAGG 1 cut(s) 800
BstF5I GGATG 6 cut(s) 159, 832, 968, 1042, 1399, 1429
BstFNI CGCG 1 cut(s) 702
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 2 cut(s) 287, 306
BstKTI GATC 9 cut(s) 33, 127, 265, 872, 1011, 1029, 1096, 1362, 1598
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 9 cut(s) 30, 124, 262, 869, 1008, 1026, 1093, 1359, 1595
BstMCI CGRYCG 1 cut(s) 1362
BstMWI GCNNNNNNNGC 6 cut(s) 92, 113, 282, 1136, 1340, 1424
BstNSI RCATGY 2 cut(s) 476, 489
BstPAI GACNNNNGTC 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 508
BstUI CGCG 1 cut(s) 702
BstV1I GCAGC 4 cut(s) 29, 32, 98, 218
BstX2I RGATCY 1 cut(s) 1026
BstYI RGATCY 1 cut(s) 1026
BstZ17I GTATAC 1 cut(s) 888
BsuI GTATCC 2 cut(s) 205, 216
BsuRI GGCC 7 cut(s) 40, 53, 627, 699, 794, 834, 1205
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 6 cut(s) 159, 832, 968, 1042, 1399, 1429
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 2 cut(s) 90, 1004
Cac8I GCNNGC 6 cut(s) 109, 487, 664, 681, 1002, 1332
CaiI CAGNNNCTG 2 cut(s) 92, 914
CfoI GCGC 2 cut(s) 287, 306
Cfr13I GGNCC 4 cut(s) 172, 584, 697, 793
CseI GACGC 1 cut(s) 1349
Csp6I GTAC 3 cut(s) 547, 923, 1554
CviAII CATG 3 cut(s) 28, 473, 486
CviQI GTAC 3 cut(s) 547, 923, 1554
DpnI GATC 9 cut(s) 32, 126, 264, 871, 1010, 1028, 1095, 1361, 1597
DpnII GATC 9 cut(s) 30, 124, 262, 869, 1008, 1026, 1093, 1359, 1595
DraI TTTAAA 1 cut(s) 1218
EaeI YGGCCR 1 cut(s) 625
Eam1104I CTCTTC 2 cut(s) 73, 1038
EarI CTCTTC 2 cut(s) 73, 1038
Ecl136II GAGCTC 1 cut(s) 505
Eco130I CCWWGG 3 cut(s) 622, 769, 835
Eco24I GRGCYC 2 cut(s) 507, 1002
Eco32I GATATC 1 cut(s) 330
Eco47I GGWCC 2 cut(s) 172, 584
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 6 cut(s) 309, 374, 609, 1062, 1110, 1512
EcoICRI GAGCTC 1 cut(s) 505
EcoNI CCTNNNNNAGG 1 cut(s) 800
EcoO109I RGGNCCY 2 cut(s) 172, 793
EcoRI GAATTC 1 cut(s) 967
EcoRV GATATC 1 cut(s) 330
EcoT14I CCWWGG 3 cut(s) 622, 769, 835
EcoT22I ATGCAT 1 cut(s) 1442
EcoT38I GRGCYC 2 cut(s) 507, 1002
ErhI CCWWGG 3 cut(s) 622, 769, 835
FaeI CATG 3 cut(s) 31, 476, 489
FalI AAGNNNNNCTT 2 cut(s) 1035, 1067
FaqI GGGAC 1 cut(s) 185
FatI CATG 3 cut(s) 27, 472, 485
FbaI TGATCA 2 cut(s) 30, 124
FblI GTMKAC 1 cut(s) 887
Fnu4HI GCNGC 6 cut(s) 18, 21, 38, 87, 207, 700
FokI GGATG 6 cut(s) 166, 839, 975, 1049, 1406, 1436
FriOI GRGCYC 2 cut(s) 507, 1002
Fsp4HI GCNGC 6 cut(s) 18, 21, 38, 87, 207, 700
FspBI CTAG 3 cut(s) 497, 836, 1131
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 2 cut(s) 286, 305
GluI GCNGC 6 cut(s) 18, 21, 38, 87, 207, 700
GsaI CCCAGC 1 cut(s) 1026
GsuI CTGGAG 1 cut(s) 590
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 7 cut(s) 40, 53, 627, 699, 794, 834, 1205
HapII CCGG 1 cut(s) 198
HgaI GACGC 1 cut(s) 1349
HhaI GCGC 2 cut(s) 287, 306
Hin1II CATG 3 cut(s) 31, 476, 489
Hin6I GCGC 2 cut(s) 285, 304
HinP1I GCGC 2 cut(s) 285, 304
HindIII AAGCTT 3 cut(s) 416, 650, 1534
HpaII CCGG 1 cut(s) 198
HphI GGTGA 1 cut(s) 1395
Hpy166II GTNNAC 3 cut(s) 80, 888, 1403
Hpy188I TCNGA 5 cut(s) 724, 790, 815, 882, 1074
Hpy188III TCNNGA 9 cut(s) 266, 587, 803, 859, 1052, 1064, 1088, 1102, 1264
Hpy8I GTNNAC 3 cut(s) 80, 888, 1403
Hpy99I CGWCG 1 cut(s) 1415
HpyAV CCTTC 4 cut(s) 275, 398, 443, 1290
HpyCH4III ACNGT 6 cut(s) 131, 238, 320, 757, 982, 1242
HpyCH4IV ACGT 3 cut(s) 921, 1410, 1562
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 113, 282, 1136, 1340, 1424
HpySE526I ACGT 3 cut(s) 921, 1410, 1562
Hsp92II CATG 3 cut(s) 31, 476, 489
HspAI GCGC 2 cut(s) 285, 304
KflI GGGWCCC 1 cut(s) 172
Ksp22I TGATCA 2 cut(s) 30, 124
Kzo9I GATC 9 cut(s) 30, 124, 262, 869, 1008, 1026, 1093, 1359, 1595
LguI GCTCTTC 1 cut(s) 1038
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 4 cut(s) 29, 32, 98, 218
LweI GCATC 7 cut(s) 488, 1027, 1057, 1414, 1427, 1449, 1534
MaeI CTAG 3 cut(s) 497, 836, 1131
MaeII ACGT 3 cut(s) 921, 1410, 1562
MaeIII GTNAC 3 cut(s) 1462, 1485, 1563
MalI GATC 9 cut(s) 32, 126, 264, 871, 1010, 1028, 1095, 1361, 1597
MboI GATC 9 cut(s) 30, 124, 262, 869, 1008, 1026, 1093, 1359, 1595
MboII GAAGA 7 cut(s) 60, 602, 668, 700, 1055, 1103, 1279
MfeI CAATTG 2 cut(s) 1185, 1476
MflI RGATCY 1 cut(s) 1026
MhlI GDGCHC 2 cut(s) 507, 1002
MlsI TGGCCA 1 cut(s) 627
MluNI TGGCCA 1 cut(s) 627
MlyI GAGTC 3 cut(s) 851, 1232, 1590
MmeI TCCRAC 3 cut(s) 128, 381, 1176
Mox20I TGGCCA 1 cut(s) 627
Mph1103I ATGCAT 1 cut(s) 1442
MroXI GAANNNNTTC 1 cut(s) 1047
MscI TGGCCA 1 cut(s) 627
MseI TTAA 8 cut(s) 516, 693, 714, 953, 1031, 1217, 1512, 1538
MslI CAYNNNNRTG 1 cut(s) 484
Msp20I TGGCCA 1 cut(s) 627
MspA1I CMGCKG 2 cut(s) 992, 1022
MspI CCGG 1 cut(s) 198
MunI CAATTG 2 cut(s) 1185, 1476
Mva1269I GAATGC 1 cut(s) 870
MvnI CGCG 1 cut(s) 702
MwoI GCNNNNNNNGC 6 cut(s) 92, 113, 282, 1136, 1340, 1424
NdeII GATC 9 cut(s) 30, 124, 262, 869, 1008, 1026, 1093, 1359, 1595
NlaIII CATG 3 cut(s) 31, 476, 489
NlaIV GGNNCC 3 cut(s) 173, 174, 468
NmuCI GTSAC 1 cut(s) 1563
NsbI TGCGCA 1 cut(s) 305
NsiI ATGCAT 1 cut(s) 1442
NspI RCATGY 2 cut(s) 476, 489
PaeI GCATGC 1 cut(s) 489
PciI ACATGT 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 1038
PctI GAATGC 1 cut(s) 870
PdmI GAANNNNTTC 1 cut(s) 1047
PfeI GAWTC 7 cut(s) 254, 575, 785, 806, 934, 1270, 1382
PkrI GCNGC 6 cut(s) 19, 22, 39, 88, 208, 701
Ple19I CGATCG 1 cut(s) 1362
PleI GAGTC 3 cut(s) 850, 1231, 1589
PpsI GAGTC 3 cut(s) 850, 1231, 1589
PpuMI RGGWCCY 1 cut(s) 172
PscI ACATGT 1 cut(s) 472
PshAI GACNNNNGTC 1 cut(s) 177
PsiI TTATAA 2 cut(s) 986, 1532
Psp124BI GAGCTC 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 1022
PspN4I GGNNCC 3 cut(s) 173, 174, 468
PspPI GGNCC 4 cut(s) 172, 584, 697, 793
PspPPI RGGWCCY 1 cut(s) 172
PstNI CAGNNNCTG 2 cut(s) 92, 914
PsuI RGATCY 1 cut(s) 1026
PvuI CGATCG 1 cut(s) 1362
PvuII CAGCTG 1 cut(s) 992
RgaI GCGATCGC 1 cut(s) 1362
RsaI GTAC 3 cut(s) 548, 924, 1555
RsaNI GTAC 3 cut(s) 547, 923, 1554
RseI CAYNNNNRTG 1 cut(s) 484
SacI GAGCTC 1 cut(s) 507
SapI GCTCTTC 1 cut(s) 1038
SaqAI TTAA 8 cut(s) 516, 693, 714, 953, 1031, 1217, 1512, 1538
SatI GCNGC 6 cut(s) 18, 21, 38, 87, 207, 700
Sau3AI GATC 9 cut(s) 30, 124, 262, 869, 1008, 1026, 1093, 1359, 1595
Sau96I GGNCC 4 cut(s) 172, 584, 697, 793
SchI GAGTC 3 cut(s) 851, 1232, 1590
SduI GDGCHC 2 cut(s) 507, 1002
SfaAI GCGATCGC 1 cut(s) 1362
SfaNI GCATC 7 cut(s) 488, 1027, 1057, 1414, 1427, 1449, 1534
SfcI CTRYAG 1 cut(s) 508
SgfI GCGATCGC 1 cut(s) 1362
SinI GGWCC 2 cut(s) 172, 584
SmiI ATTTAAAT 1 cut(s) 1218
SmiMI CAYNNNNRTG 1 cut(s) 484
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
SphI GCATGC 1 cut(s) 489
SsiI CCGC 3 cut(s) 37, 700, 1020
SspMI CTAG 3 cut(s) 497, 836, 1131
SstI GAGCTC 1 cut(s) 507
StyI CCWWGG 3 cut(s) 622, 769, 835
SwaI ATTTAAAT 1 cut(s) 1218
TaaI ACNGT 6 cut(s) 131, 238, 320, 757, 982, 1242
TaiI ACGT 3 cut(s) 924, 1413, 1565
TaqI TCGA 2 cut(s) 1273, 1413
TatI WGTACW 1 cut(s) 546
TauI GCSGC 2 cut(s) 40, 702
TfiI GAWTC 7 cut(s) 254, 575, 785, 806, 934, 1270, 1382
Tru1I TTAA 8 cut(s) 516, 693, 714, 953, 1031, 1217, 1512, 1538
Tru9I TTAA 8 cut(s) 516, 693, 714, 953, 1031, 1217, 1512, 1538
TscAI CASTG 2 cut(s) 97, 1011
TseFI GTSAC 1 cut(s) 1563
TseI GCWGC 4 cut(s) 17, 20, 86, 206
Tsp45I GTSAC 1 cut(s) 1563
TspDTI ATGAA 3 cut(s) 170, 267, 1295
TspGWI ACGGA 3 cut(s) 35, 293, 661
TspRI CASTG 2 cut(s) 97, 1011
VpaK11BI GGWCC 2 cut(s) 172, 584
XagI CCTNNNNNAGG 1 cut(s) 800
XapI RAATTY 3 cut(s) 119, 357, 967
XceI RCATGY 2 cut(s) 476, 489
XmaJI CCTAGG 1 cut(s) 835
XmiI GTMKAC 1 cut(s) 887
XmnI GAANNNNTTC 1 cut(s) 1047
XspI CTAG 3 cut(s) 497, 836, 1131
Zsp2I ATGCAT 1 cut(s) 1442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.