Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0001965

Physical Location & Seq

Genomic Coordinates Forward (+)

8686142 .. 8693316

Gene Structure 7175 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g09820.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGGAGCTAGCTCAGCTTTGAGCTGGGTTCTACTCTGCATAGTGTTGTTGTGTCTGTGCTTTCAGCTGATACAGAGCAATGTTAGCTATGACAGGAAGGCTCTTGTCATTAATGGGCAGAGGAAAATTCTCTTTTCTGGGTCCATACACTATCCTAGAAGTACTCCTGAGATGTGGGAAGGTCTTATAGAGAAGGCCAAAGATGGTGGTTTGGATGTTATAGACACATATGTGTTTTGGAATCTTCATGAGCCTTCTCCTGGCAATTACAATTTTGAAGGGAGAAATGATTTGGTTCGCTTCATTAAGATGGTCCAAGAGGCAAGGCTTTATGTGCATCTTCGCATTGGGCCGTACATTTGTTCAGAATGGAATTTTGGAGGTTTTCCAGTTTGGCTTAAGTATGTTCCAGGCATTAGCTTCAGAACAGATAATGAGCCTTTCAAGTCAGCAATGCAAAAATTTACCAAGAAGATTGTTCAATTGATGAAGCATGAAAAGCTGTTTGAGTCCCAAGGTGGCCCCATTATCCTTTCTCAGATTGAGAACGAATATGAGCCAGAAAGCAAGCTTTTTGGTGCTGCTGGTCATGCATACATGACTTGGGCTGCAGAGATGGCTGTTGGAATGGGTACTGGTGTTCCATGGGTCATGTGCAAGGAAGAGGATGCCCCAGACCCGGTGATAAACACTTGCAATGGTTTCTATTGCGACTACTTTTCTCCAAACAAACCATACAAACCCTCACTTTGGACTGAGGCTTGGACTGGCTGGTTTACAGAATTTGGCGGTCCAATTTATAAGCGACCTGTTGAAGATTTGGCGTTTGGAGTAGCTCGATTCATTCAGAACGGAGGCTCCTTCATAAATTATTACATGTATCATGGTGGAACAAACTTTGGTAGAACTGCAGGAGGTCCATTTATTACTACCAGTTATGACTATGACGCTCCCATTGATGAATATGGCTTGATCAGGCAACCAAAACATGGCCACTTAAAGGAACTCCATAAGGCTGTTAAGTTATGTGAGCAAGCTCTGCTAAATGCTGATTCTATTGTTACATCCTTAGGGAACTATGAACAGGCACACATTTTCTCTTCCAAGTCCGGAGGCTGTGCAGCTTTCCTCTCAAACTACAATACAAAGTCAGCTGCGAGGGTGACTTTCAATAAGATGAACTATCACCTCCCACCTTGGTCCATCAGCATCCTGCCGGATTGTAAGAACGTTGTATACAACACTGCCCAAGTGGGAGTTCAAACATCCCAAAAGCAGTTTTTTCCTACTAACTCTAAGTTGCGCTTGTGGGAAACCTTCAGTGAAGACATTTCTTCAGTTGCTAGTGATACGAAACTGACGGTTATTGGTCTCTTGGATCAGTTGAACATCACCAGAGACTCAAGTGATTACTTATGGTACACAACAAATATCGACATAAAATCATCTGAATCATTTCTCCGTGGCGGTCAACATCCGACTCTGACCGTGCAATCATCTGGGGATGCTATGCATGTGTTTATCAATGGGCAGCTCTCAGGATCGTCATATGGTACTAGGAAAAACAAGAGATTCACTTTCACAGGAAATGTCAGTCTGCATGCTGGAATGAACAAAATATCACTTCTCAGTATAGCTGTTGGATTGCCGAACAATGGTCCTCATTTTGAGACACGAAATGCTGGAGTACTGGGACCAGTTGCTCTACATGGACTAGACCAAGGAAGCAGAGATTTATCATGGCAGAAATGGTCACACAAGGTGGGGCTGATAGGAGAAGCCATGAACCTGGGTTCTCCAAGTTCCATCTCAGCAGTGGATTGGACGAGGGTGAATTTAGCAGCGAAGAATCAACAGCCACTGACATGGTATAAGGTCAACTTTGATGCACCTGAAGGAGATGAACCTTTGGCTTTGGACATGGGAAGTATGGGCAAGGGTCAACTATGGATCAATGGGCAAAGCATCGGAAGATACTGGACGACTTATGCAAACGGTGATTGCAGTGCATGCAGTTATGCTGGTACCTTCAGGCCTAAGAATAAGTGCCAATCTGGTTGTAACCGTCCAACTCAACAATGGTACCATGTTCCTAGGTCCTGGCTAAAGCAATCTAAAAACCTATTGGTAGTATTTGAGGAGATTAGTGGGGATGTATCGAAAATTGCTCTTGTAAAAACTTCAGTGAGCACTGTCTGTGCTGAAGTTTTTGAAAACCACCCATATATTAGGAATTGGCATACTGACAGCGGTGGGCAACTGGAAGAGCAAAAGAAACCTATAATGAGTCTACACTGTACAGAAGGATACTCCATTTCTGCTATTAAGTTCTCAAGTTTTGGAACTCCATCTGGAAGCTGTGGGAATTTTCAGCACGGCACATGTCATGCTCCAAACTCAAAGGCTGTCATTGAGAAGAAGTGTATAGGTAAGCAGAAATGTTCGTTGACCATATCTGATGCAAATTTTGGGATGGATCCATGCCCAAGCATACTGAAAAAATTATCTGTTGAAGCTGTTTGTGCCCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0009505 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

844

Amino Acids

93.66

Weight (kDa)

8.28

Isoelectric Point (pI)

41.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 339	8.2e-122	Glycosyl hydrolases family 35
GHD	PF17834	362 - 418	7.7e-25	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	471 - 538	8.4e-08	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	621 - 707	3.8e-27	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	764 - 841	8.2e-21	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36360 AT4G36360
fragaria_vesca	FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_2g09820 FvH4_2g09820
malus_domestica	MD02G1137500.v1.1 MD10G1067800.v1.1 MD15G1251100.v1.1
prunus_persica	Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.8G089500_v2.0.a1 Prupe.8G089500_v2.0.a1
pyrus_communis	pycom02g10880 pycom10g05360 pycom15g22140
rosa_chinensis	RchiOBHm_Chr2g0100171 RchiOBHm_Chr6g0266171
rosa_laevigata	RLG00000014106 RLG00000014109 RLG00000016943
rosa_multiflora	Rmu_sc0002547.1_g000001
rosa_roxburghii	Rroxscaffold_174G00435340 Rroxscaffold_2G00142500 Rroxscaffold_7G00201780 Rroxscaffold_7G00201840
rosa_rugosa	Rorug02G0086200 Rorug06G0024500 Rorug06G0024900 Rorug06G0025000
rosa_samantha	Rh2AG135300 Rh2CG140500 Rh2DG140700 Rh6BG145900 Rh6CG142300 Rh6DG130400
rosa_wichuraiana	Rw2G010560 Rw6G012540 Rw6G012580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	801
Acc65I	GGTACC	2 cut(s)	2024, 2082
AccB1I	GGYRCC	2 cut(s)	2024, 2082
AccB7I	CCANNNNNTGG	1 cut(s)	989
AccI	GTMKAC	2 cut(s)	1236, 2290
AccIII	TCCGGA	1 cut(s)	1109
AciI	CCGC	3 cut(s)	789, 1467, 2250
AclI	AACGTT	1 cut(s)	1230
AclWI	GGATC	5 cut(s)	1386, 1549, 1958, 2471, 2484
AcoI	YGGCCR	1 cut(s)	991
AcsI	RAATTY	7 cut(s)	126, 373, 461, 782, 1834, 2365, 2464
AcuI	CTGAAG	7 cut(s)	406, 1303, 1320, 1914, 2014, 2166, 2223
AdeI	CACNNNGTG	1 cut(s)	1762
AfaI	GTAC	9 cut(s)	163, 356, 634, 1421, 1555, 1689, 2026, 2084, 2299
AfiI	CCNNNNNNNGG	6 cut(s)	260, 679, 750, 989, 1000, 1655
AflII	CTTAAG	1 cut(s)	398
AflIII	ACRYGT	2 cut(s)	876, 2381
AgsI	TTSAA	9 cut(s)	278, 445, 482, 815, 1171, 1262, 1387, 2213, 2513
AjnI	CCWGG	4 cut(s)	259, 409, 1788, 2099
AjuI	GAANNNNNNNTTGG	4 cut(s)	360, 392, 1242, 1274
AleI	CACNNNNGTG	1 cut(s)	230
AloI	GAACNNNNNNTCC	2 cut(s)	842, 874
Alw21I	GWGCWC	1 cut(s)	2192
Alw26I	GTCTC	3 cut(s)	1376, 1392, 1664
AlwI	GGATC	5 cut(s)	1386, 1549, 1958, 2471, 2484
AlwNI	CAGNNNCTG	1 cut(s)	1861
Aor13HI	TCCGGA	1 cut(s)	1109
AoxI	GGCC	5 cut(s)	195, 350, 520, 991, 2033
ApeKI	GCWGC	6 cut(s)	581, 608, 1121, 1154, 1531, 1841
ApoI	RAATTY	7 cut(s)	126, 373, 461, 782, 1834, 2365, 2464
AseI	ATTAAT	1 cut(s)	111
Asp700I	GAANNNNTTC	1 cut(s)	1455
Asp718I	GGTACC	2 cut(s)	2024, 2082
AspA2I	CCTAGG	1 cut(s)	2093
AspLEI	GCGC	1 cut(s)	1305
AsuC2I	CCSGG	1 cut(s)	680
AsuHPI	GGTGA	6 cut(s)	694, 1174, 1178, 1384, 1843, 2009
AsuNHI	GCTAGC	1 cut(s)	8
AvaII	GGWCC	8 cut(s)	141, 313, 791, 917, 1200, 1658, 1694, 2097
AvrII	CCTAGG	1 cut(s)	2093
AxyI	CCTNAGG	1 cut(s)	1069
BaeGI	GKGCMC	1 cut(s)	2527
BaeI	ACNNNNGTAYC	4 cut(s)	624, 657, 2066, 2099
BalI	TGGCCA	1 cut(s)	993
BamHI	GGATCC	1 cut(s)	2476
BanI	GGYRCC	2 cut(s)	2024, 2082
BbsI	GAAGAC	1 cut(s)	1332
Bbv12I	GWGCWC	1 cut(s)	2192
BbvI	GCAGC	6 cut(s)	568, 595, 1133, 1141, 1543, 1853
BccI	CCATC	7 cut(s)	197, 304, 610, 1211, 1814, 2356, 2467
BceAI	ACGGC	2 cut(s)	337, 2392
BciT130I	CCWGG	4 cut(s)	261, 411, 1790, 2101
BciVI	GTATCC	1 cut(s)	2300
BclI	TGATCA	1 cut(s)	972
BcnI	CCSGG	1 cut(s)	680
BcoDI	GTCTC	3 cut(s)	1376, 1392, 1664
BfaI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
BfmI	CTRYAG	2 cut(s)	609, 909
BfrI	CTTAAG	1 cut(s)	398
BfuI	GTATCC	1 cut(s)	2300
BisI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 1842
BlnI	CCTAGG	1 cut(s)	2093
BlpI	GCTNAGC	1 cut(s)	13
BlsI	GCNGC	6 cut(s)	583, 610, 1123, 1156, 1533, 1843
BmcAI	AGTACT	2 cut(s)	163, 1689
Bme1390I	CCNGG	5 cut(s)	261, 411, 680, 1790, 2101
Bme18I	GGWCC	8 cut(s)	141, 313, 791, 917, 1200, 1658, 1694, 2097
BmiI	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
BmrFI	CCNGG	5 cut(s)	261, 411, 680, 1790, 2101
BmrI	ACTGGG	1 cut(s)	1700
BmsI	GCATC	7 cut(s)	346, 658, 1218, 1495, 1876, 1974, 2449
BmtI	GCTAGC	1 cut(s)	12
BmuI	ACTGGG	1 cut(s)	1700
BpiI	GAAGAC	1 cut(s)	1332
BpmI	CTGGAG	1 cut(s)	1704
Bpu1102I	GCTNAGC	1 cut(s)	13
BpuEI	CTTGAG	2 cut(s)	1387, 2317
BpuMI	CCSGG	1 cut(s)	680
BsaI	GGTCTC	1 cut(s)	1376
BsaJI	CCNNGG	7 cut(s)	514, 644, 1196, 1462, 1720, 1789, 2093
BsaWI	WCCGGW	1 cut(s)	1109
BsaXI	ACNNNNNCTCC	2 cut(s)	842, 872
Bsc4I	CCNNNNNNNGG	6 cut(s)	260, 679, 750, 989, 1000, 1655
Bse1I	ACTGG	8 cut(s)	389, 640, 772, 933, 1695, 1697, 1982, 2265
Bse21I	CCTNAGG	1 cut(s)	1069
Bse3DI	GCAATG	3 cut(s)	85, 459, 703
BseAI	TCCGGA	1 cut(s)	1109
BseBI	CCWGG	4 cut(s)	261, 411, 1790, 2101
BseDI	CCNNGG	7 cut(s)	514, 644, 1196, 1462, 1720, 1789, 2093
BseGI	GGATG	9 cut(s)	220, 673, 1064, 1209, 1265, 1474, 1510, 2158, 2478
BseLI	CCNNNNNNNGG	6 cut(s)	260, 679, 750, 989, 1000, 1655
BseMI	GCAATG	3 cut(s)	85, 459, 703
BseMII	CTCAG	7 cut(s)	27, 159, 551, 747, 1551, 1642, 1824
BseNI	ACTGG	8 cut(s)	389, 640, 772, 933, 1695, 1697, 1982, 2265
BseRI	GAGGAG	1 cut(s)	2153
BseSI	GKGCMC	1 cut(s)	2527
BseXI	GCAGC	6 cut(s)	568, 595, 1133, 1141, 1543, 1853
BseYI	CCCAGC	1 cut(s)	24
BsgI	GTGCAG	1 cut(s)	1140
BshFI	GGCC	5 cut(s)	197, 352, 522, 993, 2035
BshNI	GGYRCC	2 cut(s)	2024, 2082
BsiHKAI	GWGCWC	1 cut(s)	2192
BsiSI	CCGG	3 cut(s)	680, 1110, 1217
BslFI	GGGAC	2 cut(s)	496, 1707
BslI	CCNNNNNNNGG	6 cut(s)	260, 679, 750, 989, 1000, 1655
BsmAI	GTCTC	3 cut(s)	1376, 1392, 1664
BsmFI	GGGAC	2 cut(s)	496, 1707
BsnI	GGCC	5 cut(s)	197, 352, 522, 993, 2035
Bso31I	GGTCTC	1 cut(s)	1376
Bsp1286I	GDGCHC	2 cut(s)	2192, 2527
Bsp13I	TCCGGA	1 cut(s)	1109
Bsp1407I	TGTACA	1 cut(s)	2297
Bsp143I	GATC	5 cut(s)	972, 1378, 1541, 1950, 2476
Bsp1720I	GCTNAGC	1 cut(s)	13
Bsp19I	CCATGG	1 cut(s)	644
BspACI	CCGC	3 cut(s)	789, 1467, 2250
BspANI	GGCC	5 cut(s)	197, 352, 522, 993, 2035
BspCNI	CTCAG	7 cut(s)	26, 160, 550, 748, 1550, 1641, 1823
BspEI	TCCGGA	1 cut(s)	1109
BspHI	TCATGA	1 cut(s)	247
BspLI	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
BspMAI	CTGCAG	2 cut(s)	613, 913
BspOI	GCTAGC	1 cut(s)	12
BspPI	GGATC	5 cut(s)	1386, 1549, 1958, 2471, 2484
BspQI	GCTCTTC	1 cut(s)	2259
BspT107I	GGYRCC	2 cut(s)	2024, 2082
BspTI	CTTAAG	1 cut(s)	398
BspTNI	GGTCTC	1 cut(s)	1376
BsrDI	GCAATG	3 cut(s)	85, 459, 703
BsrGI	TGTACA	1 cut(s)	2297
BsrI	ACTGG	8 cut(s)	389, 640, 772, 933, 1695, 1697, 1982, 2265
BssECI	CCNNGG	7 cut(s)	514, 644, 1196, 1462, 1720, 1789, 2093
BssMI	GATC	5 cut(s)	972, 1378, 1541, 1950, 2476
BssNAI	GTATAC	1 cut(s)	1237
BssT1I	CCWWGG	5 cut(s)	514, 644, 1196, 1720, 2093
Bst1107I	GTATAC	1 cut(s)	1237
Bst2UI	CCWGG	4 cut(s)	261, 411, 1790, 2101
Bst4CI	ACNGT	6 cut(s)	1363, 1489, 1997, 2066, 2194, 2297
Bst6I	CTCTTC	3 cut(s)	657, 1105, 2259
BstAFI	CTTAAG	1 cut(s)	398
BstAPI	GCANNNNNTGC	2 cut(s)	1039, 2010
BstAUI	TGTACA	1 cut(s)	2297
BstC8I	GCNNGC	5 cut(s)	10, 569, 1035, 1602, 2011
BstDSI	CCRYGG	2 cut(s)	644, 1462
BstF5I	GGATG	9 cut(s)	220, 673, 1064, 1209, 1265, 1474, 1510, 2158, 2478
BstHHI	GCGC	1 cut(s)	1305
BstKTI	GATC	5 cut(s)	975, 1381, 1544, 1953, 2479
BstMAI	GTCTC	3 cut(s)	1376, 1392, 1664
BstMBI	GATC	5 cut(s)	972, 1378, 1541, 1950, 2476
BstMWI	GCNNNNNNNGC	9 cut(s)	14, 84, 334, 499, 590, 617, 1039, 2010, 2522
BstNI	CCWGG	4 cut(s)	261, 411, 1790, 2101
BstNSI	RCATGY	5 cut(s)	880, 1517, 1604, 2013, 2385
BstSCI	CCNGG	5 cut(s)	259, 409, 678, 1788, 2099
BstSFI	CTRYAG	2 cut(s)	609, 909
BstSLI	GKGCMC	1 cut(s)	2527
BstV1I	GCAGC	6 cut(s)	568, 595, 1133, 1141, 1543, 1853
BstV2I	GAAGAC	1 cut(s)	1332
BstX2I	RGATCY	1 cut(s)	2476
BstXI	CCANNNNNNTGG	2 cut(s)	1789, 1866
BstYI	RGATCY	1 cut(s)	2476
BstZ17I	GTATAC	1 cut(s)	1237
Bsu36I	CCTNAGG	1 cut(s)	1069
BsuI	GTATCC	1 cut(s)	2300
BsuRI	GGCC	5 cut(s)	197, 352, 522, 993, 2035
BtgI	CCRYGG	2 cut(s)	644, 1462
BtsCI	GGATG	9 cut(s)	220, 673, 1064, 1209, 1265, 1474, 1510, 2158, 2478
BtsI	GCAGTG	3 cut(s)	1242, 1821, 2011
BtsIMutI	CAGTG	8 cut(s)	1242, 1327, 1821, 1859, 2011, 2190, 2190, 2293
Cac8I	GCNNGC	5 cut(s)	10, 569, 1035, 1602, 2011
CaiI	CAGNNNCTG	1 cut(s)	1861
CciI	TCATGA	1 cut(s)	247
CfoI	GCGC	1 cut(s)	1305
CseI	GACGC	1 cut(s)	956
Csp6I	GTAC	9 cut(s)	162, 355, 633, 1420, 1554, 1688, 2025, 2083, 2298
CspCI	CAANNNNNGTGG	2 cut(s)	187, 222
CviQI	GTAC	9 cut(s)	162, 355, 633, 1420, 1554, 1688, 2025, 2083, 2298
DpnI	GATC	5 cut(s)	974, 1380, 1543, 1952, 2478
DpnII	GATC	5 cut(s)	972, 1378, 1541, 1950, 2476
DraIII	CACNNNGTG	1 cut(s)	1762
EaeI	YGGCCR	1 cut(s)	991
Eam1104I	CTCTTC	3 cut(s)	657, 1105, 2259
EarI	CTCTTC	3 cut(s)	657, 1105, 2259
Eco130I	CCWWGG	5 cut(s)	514, 644, 1196, 1720, 2093
Eco147I	AGGCCT	1 cut(s)	2035
Eco31I	GGTCTC	1 cut(s)	1376
Eco47I	GGWCC	8 cut(s)	141, 313, 791, 917, 1200, 1658, 1694, 2097
Eco57I	CTGAAG	7 cut(s)	406, 1303, 1320, 1914, 2014, 2166, 2223
Eco81I	CCTNAGG	1 cut(s)	1069
EcoO109I	RGGNCCY	1 cut(s)	2097
EcoRII	CCWGG	4 cut(s)	259, 409, 1788, 2099
EcoT14I	CCWWGG	5 cut(s)	514, 644, 1196, 1720, 2093
EcoT22I	ATGCAT	2 cut(s)	595, 1515
ErhI	CCWWGG	5 cut(s)	514, 644, 1196, 1720, 2093
FalI	AAGNNNNNCTT	4 cut(s)	1289, 1321, 1865, 1897
FaqI	GGGAC	2 cut(s)	496, 1707
FauNDI	CATATG	2 cut(s)	229, 1549
FbaI	TGATCA	1 cut(s)	972
FblI	GTMKAC	2 cut(s)	1236, 2290
Fnu4HI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 1842
FokI	GGATG	9 cut(s)	227, 680, 1051, 1196, 1252, 1461, 1517, 2165, 2485
Fsp4HI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 1842
FspBI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
GlaI	GCGC	1 cut(s)	1304
GluI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 1842
GsaI	CCCAGC	1 cut(s)	28
GsuI	CTGGAG	1 cut(s)	1704
HaeIII	GGCC	5 cut(s)	197, 352, 522, 993, 2035
HapII	CCGG	3 cut(s)	680, 1110, 1217
HgaI	GACGC	1 cut(s)	956
HhaI	GCGC	1 cut(s)	1305
Hin6I	GCGC	1 cut(s)	1303
HinP1I	GCGC	1 cut(s)	1303
HincII	GTYRAC	4 cut(s)	1472, 1879, 1943, 2448
HindII	GTYRAC	4 cut(s)	1472, 1879, 1943, 2448
HindIII	AAGCTT	1 cut(s)	569
HpaII	CCGG	3 cut(s)	680, 1110, 1217
HphI	GGTGA	6 cut(s)	694, 1174, 1178, 1384, 1843, 2009
Hpy166II	GTNNAC	8 cut(s)	777, 1237, 1422, 1472, 1879, 1943, 2291, 2448
Hpy188I	TCNGA	9 cut(s)	367, 425, 540, 849, 1450, 1479, 1485, 1970, 2458
Hpy188III	TCNNGA	5 cut(s)	167, 248, 1110, 1539, 2352
Hpy8I	GTNNAC	8 cut(s)	777, 1237, 1422, 1472, 1879, 1943, 2291, 2448
HpyCH4III	ACNGT	6 cut(s)	1363, 1489, 1997, 2066, 2194, 2297
HpyCH4IV	ACGT	1 cut(s)	1230
HpyF10VI	GCNNNNNNNGC	9 cut(s)	14, 84, 334, 499, 590, 617, 1039, 2010, 2522
HpySE526I	ACGT	1 cut(s)	1230
HspAI	GCGC	1 cut(s)	1303
Kpn2I	TCCGGA	1 cut(s)	1109
KpnI	GGTACC	2 cut(s)	2028, 2086
Ksp22I	TGATCA	1 cut(s)	972
Kzo9I	GATC	5 cut(s)	972, 1378, 1541, 1950, 2476
LguI	GCTCTTC	1 cut(s)	2259
LmnI	GCTCC	4 cut(s)	5, 863, 955, 2395
Lsp1109I	GCAGC	6 cut(s)	568, 595, 1133, 1141, 1543, 1853
LweI	GCATC	7 cut(s)	346, 658, 1218, 1495, 1876, 1974, 2449
MaeI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
MaeII	ACGT	1 cut(s)	1230
MaeIII	GTNAC	4 cut(s)	1060, 1162, 1752, 2060
MalI	GATC	5 cut(s)	974, 1380, 1543, 1952, 2478
MboI	GATC	5 cut(s)	972, 1378, 1541, 1950, 2476
MfeI	CAATTG	1 cut(s)	482
MflI	RGATCY	1 cut(s)	2476
MhlI	GDGCHC	2 cut(s)	2192, 2527
MlsI	TGGCCA	1 cut(s)	993
MluNI	TGGCCA	1 cut(s)	993
MlyI	GAGTC	4 cut(s)	518, 1394, 1474, 2296
MmeI	TCCRAC	4 cut(s)	604, 1502, 1621, 2093
Mox20I	TGGCCA	1 cut(s)	993
Mph1103I	ATGCAT	2 cut(s)	595, 1515
MroI	TCCGGA	1 cut(s)	1109
MroXI	GAANNNNTTC	1 cut(s)	1455
MscI	TGGCCA	1 cut(s)	993
MseI	TTAA	6 cut(s)	111, 306, 399, 998, 1020, 2325
MslI	CAYNNNNRTG	3 cut(s)	230, 308, 1864
Msp20I	TGGCCA	1 cut(s)	993
MspA1I	CMGCKG	3 cut(s)	67, 1154, 2250
MspCI	CTTAAG	1 cut(s)	398
MspI	CCGG	3 cut(s)	680, 1110, 1217
MspR9I	CCNGG	5 cut(s)	261, 411, 680, 1790, 2101
MunI	CAATTG	1 cut(s)	482
MvaI	CCWGG	4 cut(s)	261, 411, 1790, 2101
MwoI	GCNNNNNNNGC	9 cut(s)	14, 84, 334, 499, 590, 617, 1039, 2010, 2522
NciI	CCSGG	1 cut(s)	680
NcoI	CCATGG	1 cut(s)	644
NdeI	CATATG	2 cut(s)	229, 1549
NdeII	GATC	5 cut(s)	972, 1378, 1541, 1950, 2476
NheI	GCTAGC	1 cut(s)	8
NlaIV	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
NmuCI	GTSAC	2 cut(s)	1162, 1752
NsiI	ATGCAT	2 cut(s)	595, 1515
NspI	RCATGY	5 cut(s)	880, 1517, 1604, 2013, 2385
OliI	CACNNNNGTG	1 cut(s)	230
PaeI	GCATGC	2 cut(s)	1604, 2013
PagI	TCATGA	1 cut(s)	247
PceI	AGGCCT	1 cut(s)	2035
PciI	ACATGT	2 cut(s)	876, 2381
PciSI	GCTCTTC	1 cut(s)	2259
PdmI	GAANNNNTTC	1 cut(s)	1455
PfeI	GAWTC	6 cut(s)	241, 840, 1052, 1451, 1572, 1849
PflMI	CCANNNNNTGG	1 cut(s)	989
PkrI	GCNGC	6 cut(s)	583, 610, 1123, 1156, 1533, 1843
PleI	GAGTC	4 cut(s)	517, 1394, 1474, 2295
PpsI	GAGTC	4 cut(s)	517, 1394, 1474, 2295
PpuMI	RGGWCCY	1 cut(s)	2097
PscI	ACATGT	2 cut(s)	876, 2381
PshBI	ATTAAT	1 cut(s)	111
PsiI	TTATAA	1 cut(s)	801
Psp1406I	AACGTT	1 cut(s)	1230
Psp5II	RGGWCCY	1 cut(s)	2097
Psp6I	CCWGG	4 cut(s)	259, 409, 1788, 2099
PspFI	CCCAGC	1 cut(s)	24
PspGI	CCWGG	4 cut(s)	259, 409, 1788, 2099
PspN4I	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
PspPPI	RGGWCCY	1 cut(s)	2097
PsrI	GAACNNNNNNTAC	2 cut(s)	1220, 1252
PstI	CTGCAG	2 cut(s)	613, 913
PstNI	CAGNNNCTG	1 cut(s)	1861
PsuI	RGATCY	1 cut(s)	2476
PvuII	CAGCTG	2 cut(s)	67, 1154
RsaI	GTAC	9 cut(s)	163, 356, 634, 1421, 1555, 1689, 2026, 2084, 2299
RsaNI	GTAC	9 cut(s)	162, 355, 633, 1420, 1554, 1688, 2025, 2083, 2298
RseI	CAYNNNNRTG	3 cut(s)	230, 308, 1864
SapI	GCTCTTC	1 cut(s)	2259
SaqAI	TTAA	6 cut(s)	111, 306, 399, 998, 1020, 2325
SatI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 1842
Sau3AI	GATC	5 cut(s)	972, 1378, 1541, 1950, 2476
ScaI	AGTACT	2 cut(s)	163, 1689
SchI	GAGTC	4 cut(s)	518, 1394, 1474, 2296
ScrFI	CCNGG	5 cut(s)	261, 411, 680, 1790, 2101
SduI	GDGCHC	2 cut(s)	2192, 2527
SfaNI	GCATC	7 cut(s)	346, 658, 1218, 1495, 1876, 1974, 2449
SfcI	CTRYAG	2 cut(s)	609, 909
SinI	GGWCC	8 cut(s)	141, 313, 791, 917, 1200, 1658, 1694, 2097
SmiMI	CAYNNNNRTG	3 cut(s)	230, 308, 1864
SmlI	CTYRAG	3 cut(s)	398, 1402, 2332
SmoI	CTYRAG	3 cut(s)	398, 1402, 2332
SphI	GCATGC	2 cut(s)	1604, 2013
SseBI	AGGCCT	1 cut(s)	2035
SsiI	CCGC	3 cut(s)	789, 1467, 2250
SspMI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
StuI	AGGCCT	1 cut(s)	2035
StyD4I	CCNGG	5 cut(s)	259, 409, 678, 1788, 2099
StyI	CCWWGG	5 cut(s)	514, 644, 1196, 1720, 2093
TaaI	ACNGT	6 cut(s)	1363, 1489, 1997, 2066, 2194, 2297
TaiI	ACGT	1 cut(s)	1233
TaqI	TCGA	3 cut(s)	838, 1434, 2159
TatI	WGTACW	3 cut(s)	161, 1687, 2297
TfiI	GAWTC	6 cut(s)	241, 840, 1052, 1451, 1572, 1849
Tru1I	TTAA	6 cut(s)	111, 306, 399, 998, 1020, 2325
Tru9I	TTAA	6 cut(s)	111, 306, 399, 998, 1020, 2325
TscAI	CASTG	8 cut(s)	1249, 1327, 1821, 1866, 2011, 2190, 2197, 2300
TseFI	GTSAC	2 cut(s)	1162, 1752
TseI	GCWGC	6 cut(s)	581, 608, 1121, 1154, 1531, 1841
Tsp45I	GTSAC	2 cut(s)	1162, 1752
TspGWI	ACGGA	2 cut(s)	867, 1451
TspRI	CASTG	8 cut(s)	1249, 1327, 1821, 1866, 2011, 2190, 2197, 2300
Van91I	CCANNNNNTGG	1 cut(s)	989
Vha464I	CTTAAG	1 cut(s)	398
VpaK11BI	GGWCC	8 cut(s)	141, 313, 791, 917, 1200, 1658, 1694, 2097
VspI	ATTAAT	1 cut(s)	111
XapI	RAATTY	7 cut(s)	126, 373, 461, 782, 1834, 2365, 2464
XceI	RCATGY	5 cut(s)	880, 1517, 1604, 2013, 2385
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1813, 2076
XmaJI	CCTAGG	1 cut(s)	2093
XmiI	GTMKAC	2 cut(s)	1236, 2290
XmnI	GAANNNNTTC	1 cut(s)	1455
XspI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
ZrmI	AGTACT	2 cut(s)	163, 1689
Zsp2I	ATGCAT	2 cut(s)	595, 1515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.