Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001965

Physical Location & Seq

Genomic Coordinates Forward (+)

11459294 .. 11464276

Gene Structure 4983 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016943

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2562 bp

ATGGAAACCAACTCTGTTTACAAGTTGTGCTTGTTCTTGGGCTTGGTTTGGTTCTTGGGGTTTCAGCTGGTTCAGTGTACTGTGACCTATGACAGGAAGGCCATTGTGATTAATGGGCAGAGGAGAATCCTCATGTCTGGTTCAATACACTACCCCAGAAGCACTCCTGAGATGTGGGAGGATCTGATACAAAAGGCAAAAGATGGAGGTCTTGACGTGGTTGAAACCTATGTGTTCTGGAATGGCCATGAACCTACTCCTGGCAATTTCAATTTTGAAGGGAGGTATGATCTGGTGAGATTCCTGAAGACCATACAGAAAGCAGGGCTCTATGCTCATCTTCGCATTGGGCCTTATGTTTGTGCTGAGTGGAATTTTGGGGGTTTTCCTGTTTGGCTGAAATATGTTCCAGGCATCAGTTTCAGAACAGACAATGAGCCTTTCAAGAGGGCAATGCAAGGGTTTACTGAGAAGATTGTGGGACTGATGAAGAGTGAAAAATTGTTCGAGTCCCAGGGAGGCCCCATTATTCTATCACAGATTGAGAATGAGTACGGAGTACAGAGCAAGTTATTTGGAGCTGCTGGACATAATTACATGACCTGGGCAGCAGAGATGGCTGTTGGGTTGGGAACTGGGGTCCCATGGGTGATGTGCAAGGAAGAAGATGCTCCAGATCCAGTGATAAACACATGTAATGGTTTCTACTGTGATTCTTTCTCTCCCAACAAACCATACAAGCCCACAATTTGGACAGAGGCCTGGAGTGGCTGGTTTACGGAGTTTGGAGGTCCAATTCACCAACGACCAGTTCAAGATTTAGCATATGCAGTGGCCAGATTCATACAGAACGGAGGATCATTTGTTAATTATTACATGTACCATGGAGGAACCAACTTTGGACGCACAGCAGGGGGACCTTTCGTCACTACCAGCTACGACTATGATGCTCCATTAGATGAATATGGTTTAATCAGGCAGCCAAAGTATGGTCATTTGAAGGAGCTTCATAAGGCTATTAAGATGTGTGAACAGGCTTTAGTTTCAGCTGATCCAATCATCACTTCACTCGGGGACTTTCAACAGGCTCATGTGTATACTTCAGAATCAGGAGATTGTGCAGCATTTCTTTCAAACCACAATTCCAAGTCAGCTGCAAGGGTGATGTTTAATAACATGCACTATAACCTGCCTCCATGGTCAATCAGTATCCTTCCTGATTGCAGAAATGTAGTCTTTAACACTGCAAAGGTTGGAGTTCAAACATCACAAATGCAAATGTTGCCAACAAATGTTGAGACGCTATCATGGGAGACGTATGATGAAGATCTTACATCTCTAGATGACAGCTCAACGTTCACTGCTCCAGGTCTGTTGGAACAGATAAATGTTACAAGGGATACTACTGATTACCTGTGGTACATAACTAGCGTTGACATTGGTTCATCTGAATCCTTCCTGCGTGGACGGGAACTCCCAACTCTTATTGTTCAGTCAACAGGTCACGCTGTGCACATCTTCATTAATGGACAGCTTTCAGGTTCTGCCTTTGGAACAAGGGAGAATAGGAAATTCACATATACCGGCAAAGTCAATCTACGTGCAGGAACCAACAAAATTGCGCTGCTCAGTGTTGCTGTTGGATTGCCAAATGTGGGCGGACACTACGAAGCATGGAATACAGGAATCCTTGGCCCAGTTGCACTTCGTGGACTTGACCAGGGAAAATGGGACTTGTCTTGGCAGAAATGGACCTACCAGGTTGGATTGAAAGGAGAAGCCATGAGCCTTGTATCTCTGAACAGTATTTCCTCTGTTGAATGGCTGCAAGCATCATTAGCTGCTCAAAAACAGCAGCCATTGACTTGGCATAAGAGCTTTTTCAATGCACCTGACGGAGATGAGCCATTGGCTTTGGATATGGAGGGAATGGGAAAAGGCCAGATATGGATTAACGGGCAGAGTATTGGAAGATACTGGACTGCATTTGCTAATGGTGATTGCAATGGGTGCAGTTATGCTGGAGGTTTCAGACCTACAAAGTGTCAAACAGGTTGTGGTCAACCCACACAAAGATACTATCATGTGCCTCGGTCTTGGTTAAAGCCAATGCAAAATCTGTTGGTAATTTTTGAGGAACTTGGAGGCGATCCTTCAAGAATTTCTATTGTAAAGAGATCTGTGAGCACTGTCTGCGCTGACGTTGCTGAGTTCCATCCAACAATAAAGAACTGGCACATTGAAAGCTATGGAAAGGTAGAAGATTTTCACAGCCCCAAGGTTCACTTGAAGTGTAACCCAGGCCAGTCCATCTCTTCCATTAAATTCGCTAGTTTTGGCACACCTTTCGGGACCTGTGGAACTTACCAGCAAGGAAGTTGCCATGCTTCGACGTCATATTCTGTACTAGAGAAGAAGTGCATAGGGAAGCAAAGATGTGCAGTCACCATATCCAACAGTAACTTTGGAGATCCATGTCCGAAGGTATTGAAGCGCCTCTCAGTTGAAGCAGTTTGTGCTCCTACAACTTCAACAACTGCACAACCCAATTGGAGGGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0009505 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

854

Amino Acids

94.82

Weight (kDa)

7.04

Isoelectric Point (pI)

36.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 339	1.2e-122	Glycosyl hydrolases family 35
GHD	PF17834	362 - 418	2.2e-26	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	471 - 538	3.4e-07	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	621 - 706	1.5e-24	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	763 - 839	5.7e-21	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36360 AT4G36360
fragaria_vesca	FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_2g09820 FvH4_2g09820
malus_domestica	MD02G1137500.v1.1 MD10G1067800.v1.1 MD15G1251100.v1.1
prunus_persica	Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.8G089500_v2.0.a1 Prupe.8G089500_v2.0.a1
pyrus_communis	pycom02g10880 pycom10g05360 pycom15g22140
rosa_chinensis	RchiOBHm_Chr2g0100171 RchiOBHm_Chr6g0266171
rosa_laevigata	RLG00000014106 RLG00000014109 RLG00000016943
rosa_multiflora	Rmu_sc0002547.1_g000001
rosa_roxburghii	Rroxscaffold_174G00435340 Rroxscaffold_2G00142500 Rroxscaffold_7G00201780 Rroxscaffold_7G00201840
rosa_rugosa	Rorug02G0086200 Rorug06G0024500 Rorug06G0024900 Rorug06G0025000
rosa_samantha	Rh2AG135300 Rh2CG140500 Rh2DG140700 Rh6BG145900 Rh6CG142300 Rh6DG130400
rosa_wichuraiana	Rw2G010560 Rw6G012540 Rw6G012580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2396
Acc36I	ACCTGC	1 cut(s)	1197
AccB7I	CCANNNNNTGG	2 cut(s)	750, 989
AccI	GTMKAC	1 cut(s)	1097
AciI	CCGC	1 cut(s)	1659
AclI	AACGTT	1 cut(s)	1355
AclWI	GGATC	6 cut(s)	189, 671, 865, 1046, 2144, 2465
AcoI	YGGCCR	2 cut(s)	244, 834
AcsI	RAATTY	4 cut(s)	373, 1571, 2160, 2324
AcuI	CTGAAG	2 cut(s)	326, 1086
AcyI	GRCGYC	1 cut(s)	2393
AdeI	CACNNNGTG	2 cut(s)	1510, 1709
AfaI	GTAC	6 cut(s)	79, 554, 561, 881, 1421, 2406
AfiI	CCNNNNNNNGG	6 cut(s)	93, 260, 750, 989, 1655, 2553
AflIII	ACRYGT	2 cut(s)	692, 876
AhdI	GACNNNNNGTC	1 cut(s)	923
AjiI	CACGTC	1 cut(s)	217
AjnI	CCWGG	9 cut(s)	259, 409, 513, 602, 761, 1366, 1719, 1758, 2299
AjuI	GAANNNNNNNTTGG	4 cut(s)	795, 827, 1048, 1080
AloI	GAACNNNNNNTCC	2 cut(s)	1793, 1825
Alw21I	GWGCWC	3 cut(s)	1515, 2189, 2521
Alw26I	GTCTC	2 cut(s)	1292, 1307
Alw44I	GTGCAC	1 cut(s)	1511
AlwI	GGATC	6 cut(s)	189, 671, 865, 1046, 2144, 2465
AlwNI	CAGNNNCTG	1 cut(s)	1544
Ama87I	CYCGRG	1 cut(s)	1070
AoxI	GGCC	9 cut(s)	99, 244, 350, 520, 759, 834, 1693, 1939, 2302
ApaLI	GTGCAC	1 cut(s)	1511
ApeKI	GCWGC	9 cut(s)	581, 608, 979, 1121, 1154, 1624, 1825, 1841, 1855
ApoI	RAATTY	4 cut(s)	373, 1571, 2160, 2324
AseI	ATTAAT	2 cut(s)	111, 1524
Asp700I	GAANNNNTTC	1 cut(s)	2265
AspLEI	GCGC	3 cut(s)	1624, 2198, 2496
AspS9I	GGNCC	8 cut(s)	350, 521, 640, 791, 917, 1694, 1752, 2352
AsuHPI	GGTGA	6 cut(s)	307, 661, 791, 1174, 2009, 2437
AvaI	CYCGRG	1 cut(s)	1070
AvaII	GGWCC	5 cut(s)	640, 791, 917, 1752, 2352
BaeGI	GKGCMC	1 cut(s)	1515
BalI	TGGCCA	2 cut(s)	246, 836
BanII	GRGCYC	1 cut(s)	330
BbsI	GAAGAC	1 cut(s)	314
Bbv12I	GWGCWC	3 cut(s)	1515, 2189, 2521
BbvI	GCAGC	9 cut(s)	568, 620, 991, 1133, 1141, 1611, 1812, 1828, 1867
BccI	CCATC	4 cut(s)	197, 610, 2223, 2318
BcgI	CGANNNNNNTGC	4 cut(s)	929, 963, 2329, 2363
BciT130I	CCWGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
BciVI	GTATCC	2 cut(s)	1220, 1393
BcoDI	GTCTC	2 cut(s)	1292, 1307
BfaI	CTAG	4 cut(s)	1340, 1428, 2331, 2408
BfoI	RGCGCY	1 cut(s)	2497
BfuAI	ACCTGC	1 cut(s)	1197
BfuI	GTATCC	2 cut(s)	1220, 1393
BglII	AGATCT	2 cut(s)	1327, 2177
BisI	GCNGC	9 cut(s)	582, 609, 980, 1122, 1155, 1625, 1826, 1842, 1856
BlsI	GCNGC	9 cut(s)	583, 610, 981, 1123, 1156, 1626, 1827, 1843, 1857
Bme1390I	CCNGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
Bme18I	GGWCC	5 cut(s)	640, 791, 917, 1752, 2352
BmeRI	GACNNNNNGTC	1 cut(s)	923
BmeT110I	CYCGRG	1 cut(s)	1070
BmgBI	CACGTC	1 cut(s)	217
BmgT120I	GGNCC	8 cut(s)	350, 521, 640, 791, 917, 1694, 1752, 2352
BmiI	GGNNCC	7 cut(s)	523, 641, 642, 892, 918, 1609, 2353
BmrFI	CCNGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
BmrI	ACTGGG	2 cut(s)	645, 1691
BmsI	GCATC	4 cut(s)	423, 658, 937, 1841
BmuI	ACTGGG	2 cut(s)	645, 1691
BpiI	GAAGAC	1 cut(s)	314
BpmI	CTGGAG	4 cut(s)	657, 784, 1350, 2043
BsaAI	YACGTR	1 cut(s)	1601
BsaBI	GATNNNNATC	1 cut(s)	1326
BsaHI	GRCGYC	1 cut(s)	2393
BsaXI	ACNNNNNCTCC	2 cut(s)	1458, 1488
Bsc4I	CCNNNNNNNGG	6 cut(s)	93, 260, 750, 989, 1655, 2553
Bse118I	RCCGGY	1 cut(s)	1583
Bse1I	ACTGG	7 cut(s)	640, 680, 809, 1697, 1982, 2237, 2305
Bse3DI	GCAATG	2 cut(s)	459, 2011
Bse8I	GATNNNNATC	1 cut(s)	1326
BseBI	CCWGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
BseGI	GGATG	1 cut(s)	2215
BseJI	GATNNNNATC	1 cut(s)	1326
BseLI	CCNNNNNNNGG	6 cut(s)	93, 260, 750, 989, 1655, 2553
BseMI	GCAATG	2 cut(s)	459, 2011
BseMII	CTCAG	6 cut(s)	159, 357, 459, 1642, 2199, 2514
BseNI	ACTGG	7 cut(s)	640, 680, 809, 1697, 1982, 2237, 2305
BseRI	GAGGAG	1 cut(s)	136
BseSI	GKGCMC	1 cut(s)	1515
BseXI	GCAGC	9 cut(s)	568, 620, 991, 1133, 1141, 1611, 1812, 1828, 1867
BsgI	GTGCAG	5 cut(s)	1140, 1623, 2032, 2460, 2523
BshFI	GGCC	9 cut(s)	101, 246, 352, 522, 761, 836, 1695, 1941, 2304
BsiHKAI	GWGCWC	3 cut(s)	1515, 2189, 2521
BsiHKCI	CYCGRG	1 cut(s)	1070
BsiSI	CCGG	1 cut(s)	1584
BslFI	GGGAC	7 cut(s)	495, 496, 626, 930, 1088, 1745, 2365
BslI	CCNNNNNNNGG	6 cut(s)	93, 260, 750, 989, 1655, 2553
BsmAI	GTCTC	2 cut(s)	1292, 1307
BsmBI	CGTCTC	2 cut(s)	1292, 1307
BsmFI	GGGAC	7 cut(s)	495, 496, 626, 930, 1088, 1745, 2365
BsnI	GGCC	9 cut(s)	101, 246, 352, 522, 761, 836, 1695, 1941, 2304
BsoBI	CYCGRG	1 cut(s)	1070
Bsp1286I	GDGCHC	4 cut(s)	330, 1515, 2189, 2521
Bsp143I	GATC	9 cut(s)	181, 289, 676, 857, 1051, 1327, 2149, 2177, 2470
Bsp19I	CCATGG	3 cut(s)	644, 883, 1196
BspACI	CCGC	1 cut(s)	1659
BspANI	GGCC	9 cut(s)	101, 246, 352, 522, 761, 836, 1695, 1941, 2304
BspCNI	CTCAG	6 cut(s)	160, 358, 460, 1641, 2200, 2513
BspLI	GGNNCC	7 cut(s)	523, 641, 642, 892, 918, 1609, 2353
BspMI	ACCTGC	1 cut(s)	1197
BspPI	GGATC	6 cut(s)	189, 671, 865, 1046, 2144, 2465
BsrDI	GCAATG	2 cut(s)	459, 2011
BsrFI	RCCGGY	1 cut(s)	1583
BsrI	ACTGG	7 cut(s)	640, 680, 809, 1697, 1982, 2237, 2305
BssAI	RCCGGY	1 cut(s)	1583
BssMI	GATC	9 cut(s)	181, 289, 676, 857, 1051, 1327, 2149, 2177, 2470
BssNAI	GTATAC	1 cut(s)	1098
BssNI	GRCGYC	1 cut(s)	2393
BssT1I	CCWWGG	5 cut(s)	644, 883, 1196, 1690, 2277
Bst1107I	GTATAC	1 cut(s)	1098
Bst2UI	CCWGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
Bst4CI	ACNGT	5 cut(s)	82, 710, 1805, 2191, 2459
Bst6I	CTCTTC	2 cut(s)	485, 2320
BstACI	GRCGYC	1 cut(s)	2393
BstAPI	GCANNNNNTGC	4 cut(s)	1282, 2010, 2193, 2516
BstBAI	YACGTR	1 cut(s)	1601
BstC8I	GCNNGC	1 cut(s)	1830
BstDEI	CTNAG	6 cut(s)	168, 366, 468, 1628, 2208, 2500
BstDSI	CCRYGG	3 cut(s)	644, 883, 1196
BstENI	CCTNNNNNAGG	1 cut(s)	91
BstF5I	GGATG	1 cut(s)	2215
BstH2I	RGCGCY	1 cut(s)	2497
BstHHI	GCGC	3 cut(s)	1624, 2198, 2496
BstKTI	GATC	9 cut(s)	184, 292, 679, 860, 1054, 1330, 2152, 2180, 2473
BstMAI	GTCTC	2 cut(s)	1292, 1307
BstMBI	GATC	9 cut(s)	181, 289, 676, 857, 1051, 1327, 2149, 2177, 2470
BstMWI	GCNNNNNNNGC	7 cut(s)	617, 1282, 1838, 2010, 2193, 2204, 2516
BstNI	CCWGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
BstNSI	RCATGY	3 cut(s)	696, 880, 1180
BstSCI	CCNGG	9 cut(s)	259, 409, 513, 602, 761, 1366, 1719, 1758, 2299
BstSLI	GKGCMC	1 cut(s)	1515
BstV1I	GCAGC	9 cut(s)	568, 620, 991, 1133, 1141, 1611, 1812, 1828, 1867
BstV2I	GAAGAC	1 cut(s)	314
BstX2I	RGATCY	5 cut(s)	181, 676, 1327, 2177, 2470
BstXI	CCANNNNNNTGG	1 cut(s)	1866
BstYI	RGATCY	5 cut(s)	181, 676, 1327, 2177, 2470
BstZ17I	GTATAC	1 cut(s)	1098
BsuI	GTATCC	2 cut(s)	1220, 1393
BsuRI	GGCC	9 cut(s)	101, 246, 352, 522, 761, 836, 1695, 1941, 2304
BtgI	CCRYGG	3 cut(s)	644, 883, 1196
BtrI	CACGTC	1 cut(s)	217
BtsCI	GGATG	1 cut(s)	2215
BtsI	GCAGTG	3 cut(s)	837, 1242, 1359
BtsIMutI	CAGTG	7 cut(s)	80, 687, 837, 1242, 1359, 1636, 2187
BveI	ACCTGC	1 cut(s)	1197
Cac8I	GCNNGC	1 cut(s)	1830
CaiI	CAGNNNCTG	1 cut(s)	1544
CfoI	GCGC	3 cut(s)	1624, 2198, 2496
Cfr10I	RCCGGY	1 cut(s)	1583
Cfr13I	GGNCC	8 cut(s)	350, 521, 640, 791, 917, 1694, 1752, 2352
CseI	GACGC	2 cut(s)	912, 1309
CsiI	ACCWGGT	1 cut(s)	1758
Csp6I	GTAC	6 cut(s)	78, 553, 560, 880, 1420, 2405
CviQI	GTAC	6 cut(s)	78, 553, 560, 880, 1420, 2405
DdeI	CTNAG	6 cut(s)	168, 366, 468, 1628, 2208, 2500
DpnI	GATC	9 cut(s)	183, 291, 678, 859, 1053, 1329, 2151, 2179, 2472
DpnII	GATC	9 cut(s)	181, 289, 676, 857, 1051, 1327, 2149, 2177, 2470
DraIII	CACNNNGTG	2 cut(s)	1510, 1709
DriI	GACNNNNNGTC	1 cut(s)	923
EaeI	YGGCCR	2 cut(s)	244, 834
Eam1104I	CTCTTC	2 cut(s)	485, 2320
Eam1105I	GACNNNNNGTC	1 cut(s)	923
EarI	CTCTTC	2 cut(s)	485, 2320
EciI	GGCGGA	1 cut(s)	1674
Eco130I	CCWWGG	5 cut(s)	644, 883, 1196, 1690, 2277
Eco147I	AGGCCT	1 cut(s)	761
Eco24I	GRGCYC	1 cut(s)	330
Eco47I	GGWCC	5 cut(s)	640, 791, 917, 1752, 2352
Eco57I	CTGAAG	2 cut(s)	326, 1086
Eco88I	CYCGRG	1 cut(s)	1070
EcoNI	CCTNNNNNAGG	1 cut(s)	91
EcoO109I	RGGNCCY	4 cut(s)	521, 640, 917, 2352
EcoRII	CCWGG	9 cut(s)	259, 409, 513, 602, 761, 1366, 1719, 1758, 2299
EcoT14I	CCWWGG	5 cut(s)	644, 883, 1196, 1690, 2277
EcoT38I	GRGCYC	1 cut(s)	330
ErhI	CCWWGG	5 cut(s)	644, 883, 1196, 1690, 2277
Esp3I	CGTCTC	2 cut(s)	1292, 1307
FalI	AAGNNNNNCTT	4 cut(s)	14, 46, 2270, 2302
FaqI	GGGAC	7 cut(s)	495, 496, 626, 930, 1088, 1745, 2365
FauNDI	CATATG	1 cut(s)	826
FblI	GTMKAC	1 cut(s)	1097
Fnu4HI	GCNGC	9 cut(s)	582, 609, 980, 1122, 1155, 1625, 1826, 1842, 1856
FokI	GGATG	1 cut(s)	2202
FriOI	GRGCYC	1 cut(s)	330
Fsp4HI	GCNGC	9 cut(s)	582, 609, 980, 1122, 1155, 1625, 1826, 1842, 1856
FspBI	CTAG	4 cut(s)	1340, 1428, 2331, 2408
GlaI	GCGC	3 cut(s)	1623, 2197, 2495
GluI	GCNGC	9 cut(s)	582, 609, 980, 1122, 1155, 1625, 1826, 1842, 1856
GsuI	CTGGAG	4 cut(s)	657, 784, 1350, 2043
HaeII	RGCGCY	1 cut(s)	2497
HaeIII	GGCC	9 cut(s)	101, 246, 352, 522, 761, 836, 1695, 1941, 2304
HapII	CCGG	1 cut(s)	1584
HgaI	GACGC	2 cut(s)	912, 1309
HhaI	GCGC	3 cut(s)	1624, 2198, 2496
Hin1I	GRCGYC	1 cut(s)	2393
Hin6I	GCGC	3 cut(s)	1622, 2196, 2494
HinP1I	GCGC	3 cut(s)	1622, 2196, 2494
HincII	GTYRAC	3 cut(s)	1435, 1497, 2063
HindII	GTYRAC	3 cut(s)	1435, 1497, 2063
HinfI	GANTC	8 cut(s)	126, 300, 509, 713, 840, 1106, 1451, 1686
HpaII	CCGG	1 cut(s)	1584
HphI	GGTGA	6 cut(s)	307, 661, 791, 1174, 2009, 2437
Hpy188I	TCNGA	7 cut(s)	186, 425, 1105, 1450, 1800, 2033, 2481
Hpy99I	CGWCG	1 cut(s)	2395
HpyAV	CCTTC	7 cut(s)	91, 272, 994, 1223, 1465, 2163, 2476
HpyCH4III	ACNGT	5 cut(s)	82, 710, 1805, 2191, 2459
HpyCH4IV	ACGT	6 cut(s)	216, 1316, 1355, 1600, 2202, 2393
HpyF10VI	GCNNNNNNNGC	7 cut(s)	617, 1282, 1838, 2010, 2193, 2204, 2516
HpyF3I	CTNAG	6 cut(s)	168, 366, 468, 1628, 2208, 2500
HpySE526I	ACGT	6 cut(s)	216, 1316, 1355, 1600, 2202, 2393
Hsp92I	GRCGYC	1 cut(s)	2393
HspAI	GCGC	3 cut(s)	1622, 2196, 2494
KflI	GGGWCCC	1 cut(s)	640
Kzo9I	GATC	9 cut(s)	181, 289, 676, 857, 1051, 1327, 2149, 2177, 2470
LmnI	GCTCC	6 cut(s)	578, 676, 955, 1003, 1369, 2524
Lsp1109I	GCAGC	9 cut(s)	568, 620, 991, 1133, 1141, 1611, 1812, 1828, 1867
LweI	GCATC	4 cut(s)	423, 658, 937, 1841
MabI	ACCWGGT	1 cut(s)	1758
MaeI	CTAG	4 cut(s)	1340, 1428, 2331, 2408
MaeII	ACGT	6 cut(s)	216, 1316, 1355, 1600, 2202, 2393
MaeIII	GTNAC	7 cut(s)	82, 925, 1390, 1502, 2294, 2443, 2459
MalI	GATC	9 cut(s)	183, 291, 678, 859, 1053, 1329, 2151, 2179, 2472
MboI	GATC	9 cut(s)	181, 289, 676, 857, 1051, 1327, 2149, 2177, 2470
MfeI	CAATTG	1 cut(s)	2548
MflI	RGATCY	5 cut(s)	181, 676, 1327, 2177, 2470
MhlI	GDGCHC	4 cut(s)	330, 1515, 2189, 2521
MlsI	TGGCCA	2 cut(s)	246, 836
MluNI	TGGCCA	2 cut(s)	246, 836
MlyI	GAGTC	1 cut(s)	518
MmeI	TCCRAC	6 cut(s)	1234, 1356, 1621, 1744, 2243, 2478
Mox20I	TGGCCA	2 cut(s)	246, 836
MroXI	GAANNNNTTC	1 cut(s)	2265
MscI	TGGCCA	2 cut(s)	246, 836
Msp20I	TGGCCA	2 cut(s)	246, 836
MspA1I	CMGCKG	3 cut(s)	67, 1049, 1154
MspI	CCGG	1 cut(s)	1584
MspR9I	CCNGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
MunI	CAATTG	1 cut(s)	2548
MvaI	CCWGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
MwoI	GCNNNNNNNGC	7 cut(s)	617, 1282, 1838, 2010, 2193, 2204, 2516
NcoI	CCATGG	3 cut(s)	644, 883, 1196
NdeI	CATATG	1 cut(s)	826
NdeII	GATC	9 cut(s)	181, 289, 676, 857, 1051, 1327, 2149, 2177, 2470
NlaIV	GGNNCC	7 cut(s)	523, 641, 642, 892, 918, 1609, 2353
NmuCI	GTSAC	4 cut(s)	82, 925, 1502, 2443
NspI	RCATGY	3 cut(s)	696, 880, 1180
PasI	CCCWGGG	1 cut(s)	514
PceI	AGGCCT	1 cut(s)	761
PciI	ACATGT	2 cut(s)	692, 876
PdmI	GAANNNNTTC	1 cut(s)	2265
PfeI	GAWTC	7 cut(s)	126, 300, 713, 840, 1106, 1451, 1686
PflMI	CCANNNNNTGG	2 cut(s)	750, 989
PkrI	GCNGC	9 cut(s)	583, 610, 981, 1123, 1156, 1626, 1827, 1843, 1857
PleI	GAGTC	1 cut(s)	517
PpsI	GAGTC	1 cut(s)	517
Ppu21I	YACGTR	1 cut(s)	1601
PpuMI	RGGWCCY	3 cut(s)	640, 917, 2352
PscI	ACATGT	2 cut(s)	692, 876
PshBI	ATTAAT	2 cut(s)	111, 1524
Psp1406I	AACGTT	1 cut(s)	1355
Psp5II	RGGWCCY	3 cut(s)	640, 917, 2352
Psp6I	CCWGG	9 cut(s)	259, 409, 513, 602, 761, 1366, 1719, 1758, 2299
PspGI	CCWGG	9 cut(s)	259, 409, 513, 602, 761, 1366, 1719, 1758, 2299
PspN4I	GGNNCC	7 cut(s)	523, 641, 642, 892, 918, 1609, 2353
PspPI	GGNCC	8 cut(s)	350, 521, 640, 791, 917, 1694, 1752, 2352
PspPPI	RGGWCCY	3 cut(s)	640, 917, 2352
PstNI	CAGNNNCTG	1 cut(s)	1544
PsuI	RGATCY	5 cut(s)	181, 676, 1327, 2177, 2470
PvuII	CAGCTG	3 cut(s)	67, 1049, 1154
RsaI	GTAC	6 cut(s)	79, 554, 561, 881, 1421, 2406
RsaNI	GTAC	6 cut(s)	78, 553, 560, 880, 1420, 2405
SatI	GCNGC	9 cut(s)	582, 609, 980, 1122, 1155, 1625, 1826, 1842, 1856
Sau3AI	GATC	9 cut(s)	181, 289, 676, 857, 1051, 1327, 2149, 2177, 2470
Sau96I	GGNCC	8 cut(s)	350, 521, 640, 791, 917, 1694, 1752, 2352
SchI	GAGTC	1 cut(s)	518
ScrFI	CCNGG	9 cut(s)	261, 411, 515, 604, 763, 1368, 1721, 1760, 2301
SduI	GDGCHC	4 cut(s)	330, 1515, 2189, 2521
SexAI	ACCWGGT	1 cut(s)	1758
SfaNI	GCATC	4 cut(s)	423, 658, 937, 1841
SinI	GGWCC	5 cut(s)	640, 791, 917, 1752, 2352
SseBI	AGGCCT	1 cut(s)	761
SsiI	CCGC	1 cut(s)	1659
SspMI	CTAG	4 cut(s)	1340, 1428, 2331, 2408
StuI	AGGCCT	1 cut(s)	761
StyD4I	CCNGG	9 cut(s)	259, 409, 513, 602, 761, 1366, 1719, 1758, 2299
StyI	CCWWGG	5 cut(s)	644, 883, 1196, 1690, 2277
TaaI	ACNGT	5 cut(s)	82, 710, 1805, 2191, 2459
TaiI	ACGT	6 cut(s)	219, 1319, 1358, 1603, 2205, 2396
TaqI	TCGA	2 cut(s)	507, 2390
TaqII	GACCGA	1 cut(s)	2082
TatI	WGTACW	3 cut(s)	77, 559, 2404
TfiI	GAWTC	7 cut(s)	126, 300, 713, 840, 1106, 1451, 1686
TscAI	CASTG	7 cut(s)	80, 687, 837, 1249, 1366, 1636, 2194
TseFI	GTSAC	4 cut(s)	82, 925, 1502, 2443
TseI	GCWGC	9 cut(s)	581, 608, 979, 1121, 1154, 1624, 1825, 1841, 1855
Tsp45I	GTSAC	4 cut(s)	82, 925, 1502, 2443
TspDTI	ATGAA	8 cut(s)	264, 503, 832, 975, 998, 1338, 1434, 1510
TspGWI	ACGGA	4 cut(s)	570, 794, 867, 1911
TspRI	CASTG	7 cut(s)	80, 687, 837, 1249, 1366, 1636, 2194
Van91I	CCANNNNNTGG	2 cut(s)	750, 989
VneI	GTGCAC	1 cut(s)	1511
VpaK11BI	GGWCC	5 cut(s)	640, 791, 917, 1752, 2352
VspI	ATTAAT	2 cut(s)	111, 1524
XagI	CCTNNNNNAGG	1 cut(s)	91
XapI	RAATTY	4 cut(s)	373, 1571, 2160, 2324
XbaI	TCTAGA	1 cut(s)	1339
XceI	RCATGY	3 cut(s)	696, 880, 1180
XmiI	GTMKAC	1 cut(s)	1097
XmnI	GAANNNNTTC	1 cut(s)	2265
XspI	CTAG	4 cut(s)	1340, 1428, 2331, 2408
ZraI	GACGTC	1 cut(s)	2394

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.