Rroxscaffold_7G00201780

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001965
Physical Location & Seq
Reverse (-)
49573156 .. 49579358
6203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00201780.1

Sequence Viewer

Length: 1572 bp
ATGGAGGAACTAACTTTGGAAGAACTGCTGGAGGCCCTTTTATTATTACCAGCTATGACTACGATGCCCCCATTGATGAATATGTCACATGTATTCTCTTCAAAGCCAGGAGGTTGTGCAGCATTTCTCTCAAACTACAATGCAAACTCAGCTGCAACGGTGACTTTCAATGACCGAAACTATGAGCTCCCTGCTTGGTCTATCAGCGTCCTTCCAGACTGTGAAAATGTTGTATTCAACACTGCCAAAGTAAATGAAATAAACAATGGTCTCTCTGCAATGCTTATTTACTATTTTCCACAAAATTTGTCTGACATCAAAGTGGGAGTTCAGACATCCCAAAAACAATTTTTTCCCACTAACTCTGAGCTGTGGTCATGGGAAACCTTCAGTGAAGACATTGCTTCATGGACTGGTGATACAACTCTCACAGTCATTGGTTTCTTAGATCAGTTGAACATCACTAGAGACTCCAGTGATTATCTATGGTACACTACCAGTGTCGACATAAGTCCATCTGAATTATTTCTGCGTGGAGGTAAGCATCCAATTCTCACTGTGCAGTCTGCTGGGAACGCTATGCATGTCTTTATCAATGACCAGCTCTCAGGATCAACTTATGGGACTCTACAAGACCAGAGATTCACTTTTACAGGAGATGTAAACATGCATGCTGGTGTGAATAAAATTTCACTTCTAAGTGTAGCTGTTGGATTACCGAACAATGGTCCTCACTTTGAGACATGGAACTTGGGAGTACTAGGACCAGTTGTTCTACAAGGATTGGATCAAGGAAGCAGAGATTTATCAGGGCAGACATGGTCATACAAGGTTGGGCTAAAAGGAGAATCCATGAATCTCGGTTCTCCAGCTTCCACCTCTGCAGTGAATTGGACGAGGGTGTCTATAGGGACACAGAATCAACAGCCACTGACTTGGTATAAGGCCAATTTTGATGCACCTGAAGAGGATGAGCCTTTGGCTTTGGATATGGGGAGCATGGGGAAGGGTCAAGTATGGATCAATGGGGAGAATATTGGAAGATACTGGACTATTCATGCTAATGGTAACTGCAGCGCGTGCAGTTATTCTGGTACATTCAGGATTAACAAGTATCATGTTCCTCGGTCTTGGCTCAAGCCATCTCAAAATCTGTTGGTTGTTTTTGAGGAGCTTGCTGGCGATGTTTCAAAGATTGATCTTGTGAAAACATCAGTGAGGACCATCTGTGCCGAGATTTCGGAAAGTTACCCACTCATTAGGGAATTCGACACCAAGAGCCAAGGCAAATTGAAAGAGCAAAAGAAGCCTAAACTTAGCCTAGAGTGTAAAAATGGGCACTCTATTTCGGCTATTAAATTTTCAAGCTTCGGTACTCCATCCGGAAGCTGTGGAAACTATAAGCATTGTACTTGTCACGCTCCAAACTCTACCCCTGTGCTAAATAAGAGGTGCGTAGGTAAGCAGAAGTGTTCAGTGACTGTATCCAATGCAAACTTTGGCATGGACCCTTGCCCAGGCACAGTGAAAAAATTATCTGTGGAAGCTATTTGTGCCCCTATAACTACATGA

Protein Analysis

523

Amino Acids

57.28

Weight (kDa)

5.85

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHD PF17834 29 - 84 2.9e-24 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 161 - 228 2.4e-07 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 311 - 384 8.1e-25 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 441 - 518 1.5e-20 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 901
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 1079
AccIII TCCGGA 1 cut(s) 1382
AclWI GGATC 3 cut(s) 619, 795, 1028
AcsI RAATTY 4 cut(s) 304, 687, 1265, 1358
AcuI CTGAAG 2 cut(s) 373, 984
AfaI GTAC 5 cut(s) 491, 759, 1096, 1375, 1411
AfiI CCNNNNNNNGG 2 cut(s) 725, 1517
AflIII ACRYGT 1 cut(s) 88
AgsI TTSAA 7 cut(s) 102, 169, 238, 457, 1191, 1294, 1365
AjnI CCWGG 2 cut(s) 106, 1516
AloI GAACNNNNNNTCC 2 cut(s) 756, 788
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 3 cut(s) 275, 462, 734
AlwI GGATC 3 cut(s) 619, 795, 1028
AlwNI CAGNNNCTG 2 cut(s) 931, 1481
Aor13HI TCCGGA 1 cut(s) 1382
AoxI GGCC 2 cut(s) 33, 945
ApeKI GCWGC 3 cut(s) 119, 152, 1074
ApoI RAATTY 4 cut(s) 304, 687, 1265, 1358
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 1 cut(s) 1079
AspS9I GGNCC 5 cut(s) 34, 728, 764, 1221, 1507
AsuHPI GGTGA 2 cut(s) 172, 428
AvaII GGWCC 4 cut(s) 728, 764, 1221, 1507
BaeGI GKGCMC 2 cut(s) 1341, 1558
BanII GRGCYC 1 cut(s) 189
BbsI GAAGAC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 3 cut(s) 131, 139, 1086
BccI CCATC 4 cut(s) 523, 1150, 1232, 1387
BciT130I CCWGG 2 cut(s) 108, 1518
BciVI GTATCC 1 cut(s) 1495
BcoDI GTCTC 3 cut(s) 275, 462, 734
BfaI CTAG 3 cut(s) 465, 761, 1322
BfmI CTRYAG 3 cut(s) 882, 906, 1072
BfuI GTATCC 1 cut(s) 1495
BisI GCNGC 3 cut(s) 120, 153, 1075
BlsI GCNGC 3 cut(s) 121, 154, 1076
BmcAI AGTACT 1 cut(s) 759
Bme1390I CCNGG 2 cut(s) 108, 1518
Bme18I GGWCC 4 cut(s) 728, 764, 1221, 1507
BmgT120I GGNCC 5 cut(s) 34, 728, 764, 1221, 1507
BmiI GGNNCC 1 cut(s) 1509
BmrFI CCNGG 2 cut(s) 108, 1518
BmsI GCATC 3 cut(s) 54, 553, 946
BoxI GACNNNNGTC 1 cut(s) 510
BpiI GAAGAC 1 cut(s) 402
BpmI CTGGAG 3 cut(s) 50, 457, 852
BpuEI CTTGAG 1 cut(s) 1121
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 3 cut(s) 1124, 1282, 1516
BsaWI WCCGGW 1 cut(s) 1382
Bsc4I CCNNNNNNNGG 2 cut(s) 725, 1517
Bse1I ACTGG 5 cut(s) 418, 474, 498, 767, 1052
Bse3DI GCAATG 2 cut(s) 285, 399
BseAI TCCGGA 1 cut(s) 1382
BseBI CCWGG 2 cut(s) 108, 1518
BseDI CCNNGG 3 cut(s) 1124, 1282, 1516
BseGI GGATG 4 cut(s) 335, 544, 976, 1379
BseLI CCNNNNNNNGG 2 cut(s) 725, 1517
BseMI GCAATG 2 cut(s) 285, 399
BseMII CTCAG 3 cut(s) 162, 357, 621
BseNI ACTGG 5 cut(s) 418, 474, 498, 767, 1052
BseRI GAGGAG 1 cut(s) 1184
BseSI GKGCMC 2 cut(s) 1341, 1558
BseXI GCAGC 3 cut(s) 131, 139, 1086
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 3 cut(s) 138, 581, 1102
Bsh1236I CGCG 1 cut(s) 1079
BshFI GGCC 2 cut(s) 35, 947
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 1383
BslFI GGGAC 2 cut(s) 637, 925
BslI CCNNNNNNNGG 2 cut(s) 725, 1517
BsmAI GTCTC 3 cut(s) 275, 462, 734
BsmFI GGGAC 2 cut(s) 637, 925
BsnI GGCC 2 cut(s) 35, 947
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 3 cut(s) 189, 1341, 1558
Bsp13I TCCGGA 1 cut(s) 1382
Bsp143I GATC 5 cut(s) 448, 611, 787, 1020, 1198
BspANI GGCC 2 cut(s) 35, 947
BspCNI CTCAG 3 cut(s) 161, 358, 620
BspEI TCCGGA 1 cut(s) 1382
BspFNI CGCG 1 cut(s) 1079
BspLI GGNNCC 1 cut(s) 1509
BspMAI CTGCAG 2 cut(s) 886, 1076
BspPI GGATC 3 cut(s) 619, 795, 1028
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 2 cut(s) 285, 399
BsrI ACTGG 5 cut(s) 418, 474, 498, 767, 1052
BssECI CCNNGG 3 cut(s) 1124, 1282, 1516
BssMI GATC 5 cut(s) 448, 611, 787, 1020, 1198
BssT1I CCWWGG 1 cut(s) 1282
Bst2UI CCWGG 2 cut(s) 108, 1518
Bst4CI ACNGT 6 cut(s) 160, 221, 433, 559, 1483, 1525
Bst6I CTCTTC 2 cut(s) 103, 960
BstAPI GCANNNNNTGC 1 cut(s) 1080
BstC8I GCNNGC 4 cut(s) 672, 1081, 1176, 1180
BstDEI CTNAG 6 cut(s) 148, 366, 445, 607, 698, 1316
BstENI CCTNNNNNAGG 1 cut(s) 1515
BstF5I GGATG 4 cut(s) 335, 544, 976, 1379
BstFNI CGCG 1 cut(s) 1079
BstHHI GCGC 1 cut(s) 1079
BstKTI GATC 5 cut(s) 451, 614, 790, 1023, 1201
BstMAI GTCTC 3 cut(s) 275, 462, 734
BstMBI GATC 5 cut(s) 448, 611, 787, 1020, 1198
BstMWI GCNNNNNNNGC 5 cut(s) 149, 575, 1080, 1306, 1553
BstNI CCWGG 2 cut(s) 108, 1518
BstNSI RCATGY 4 cut(s) 92, 587, 670, 674
BstPAI GACNNNNGTC 1 cut(s) 510
BstSCI CCNGG 2 cut(s) 106, 1516
BstSFI CTRYAG 3 cut(s) 882, 906, 1072
BstSLI GKGCMC 2 cut(s) 1341, 1558
BstUI CGCG 1 cut(s) 1079
BstV1I GCAGC 3 cut(s) 131, 139, 1086
BstV2I GAAGAC 1 cut(s) 402
BstXI CCANNNNNNTGG 1 cut(s) 936
BsuI GTATCC 1 cut(s) 1495
BsuRI GGCC 2 cut(s) 35, 947
BtgZI GCGATG 1 cut(s) 1197
BtsCI GGATG 4 cut(s) 335, 544, 976, 1379
BtsI GCAGTG 2 cut(s) 240, 891
Cac8I GCNNGC 4 cut(s) 672, 1081, 1176, 1180
CaiI CAGNNNCTG 2 cut(s) 931, 1481
CfoI GCGC 1 cut(s) 1079
Cfr13I GGNCC 5 cut(s) 34, 728, 764, 1221, 1507
CseI GACGC 1 cut(s) 196
Csp6I GTAC 5 cut(s) 490, 758, 1095, 1374, 1410
CspCI CAANNNNNGTGG 2 cut(s) 288, 323
CviQI GTAC 5 cut(s) 490, 758, 1095, 1374, 1410
DdeI CTNAG 6 cut(s) 148, 366, 445, 607, 698, 1316
DpnI GATC 5 cut(s) 450, 613, 789, 1022, 1200
DpnII GATC 5 cut(s) 448, 611, 787, 1020, 1198
DrdI GACNNNNNNGTC 1 cut(s) 901
DseDI GACNNNNNNGTC 1 cut(s) 901
Eam1104I CTCTTC 2 cut(s) 103, 960
EarI CTCTTC 2 cut(s) 103, 960
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 1282
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 275
Eco47I GGWCC 4 cut(s) 728, 764, 1221, 1507
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 2 cut(s) 373, 984
EcoICRI GAGCTC 1 cut(s) 187
EcoNI CCTNNNNNAGG 1 cut(s) 1515
EcoO109I RGGNCCY 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 1265
EcoRII CCWGG 2 cut(s) 106, 1516
EcoT14I CCWWGG 1 cut(s) 1282
EcoT22I ATGCAT 2 cut(s) 585, 672
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 1282
FaqI GGGAC 2 cut(s) 637, 925
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 120, 153, 1075
FokI GGATG 4 cut(s) 322, 531, 983, 1366
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 3 cut(s) 120, 153, 1075
FspBI CTAG 3 cut(s) 465, 761, 1322
GlaI GCGC 1 cut(s) 1078
GluI GCNGC 3 cut(s) 120, 153, 1075
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 3 cut(s) 50, 457, 852
HaeIII GGCC 2 cut(s) 35, 947
HapII CCGG 1 cut(s) 1383
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 1079
Hin6I GCGC 1 cut(s) 1077
HinP1I GCGC 1 cut(s) 1077
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 1366
HinfI GANTC 6 cut(s) 470, 625, 642, 848, 856, 919
HpaII CCGG 1 cut(s) 1383
HphI GGTGA 2 cut(s) 172, 428
Hpy166II GTNNAC 3 cut(s) 492, 505, 664
Hpy188I TCNGA 5 cut(s) 313, 333, 367, 520, 1243
Hpy188III TCNNGA 4 cut(s) 215, 609, 1102, 1383
Hpy8I GTNNAC 3 cut(s) 492, 505, 664
HpyAV CCTTC 3 cut(s) 221, 397, 1000
HpyCH4III ACNGT 6 cut(s) 160, 221, 433, 559, 1483, 1525
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 575, 1080, 1306, 1553
HpyF3I CTNAG 6 cut(s) 148, 366, 445, 607, 698, 1316
HspAI GCGC 1 cut(s) 1077
Kpn2I TCCGGA 1 cut(s) 1382
Kzo9I GATC 5 cut(s) 448, 611, 787, 1020, 1198
LmnI GCTCC 4 cut(s) 192, 996, 1171, 1426
Lsp1109I GCAGC 3 cut(s) 131, 139, 1086
LweI GCATC 3 cut(s) 54, 553, 946
MaeI CTAG 3 cut(s) 465, 761, 1322
MaeIII GTNAC 6 cut(s) 84, 160, 1067, 1247, 1415, 1477
MalI GATC 5 cut(s) 450, 613, 789, 1022, 1200
MboI GATC 5 cut(s) 448, 611, 787, 1020, 1198
MboII GAAGA 5 cut(s) 32, 90, 407, 977, 1053
MhlI GDGCHC 3 cut(s) 189, 1341, 1558
MlyI GAGTC 2 cut(s) 464, 619
MmeI TCCRAC 1 cut(s) 691
Mph1103I ATGCAT 2 cut(s) 585, 672
MroI TCCGGA 1 cut(s) 1382
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 2 cut(s) 1107, 1356
MslI CAYNNNNRTG 3 cut(s) 320, 675, 1062
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 1 cut(s) 1383
MspR9I CCNGG 2 cut(s) 108, 1518
MvaI CCWGG 2 cut(s) 108, 1518
MvnI CGCG 1 cut(s) 1079
MwoI GCNNNNNNNGC 5 cut(s) 149, 575, 1080, 1306, 1553
NdeII GATC 5 cut(s) 448, 611, 787, 1020, 1198
NlaIV GGNNCC 1 cut(s) 1509
NmeAIII GCCGAG 1 cut(s) 1258
NmuCI GTSAC 4 cut(s) 84, 160, 1415, 1477
NsiI ATGCAT 2 cut(s) 585, 672
NspI RCATGY 4 cut(s) 92, 587, 670, 674
PaeI GCATGC 1 cut(s) 674
PciI ACATGT 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 4 cut(s) 642, 848, 856, 919
PflFI GACNNNGTC 1 cut(s) 820
PkrI GCNGC 3 cut(s) 121, 154, 1076
PleI GAGTC 2 cut(s) 464, 619
PpsI GAGTC 2 cut(s) 464, 619
PscI ACATGT 1 cut(s) 88
PshAI GACNNNNGTC 1 cut(s) 510
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 106, 1516
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 2 cut(s) 106, 1516
PspN4I GGNNCC 1 cut(s) 1509
PspPI GGNCC 5 cut(s) 34, 728, 764, 1221, 1507
PstI CTGCAG 2 cut(s) 886, 1076
PstNI CAGNNNCTG 2 cut(s) 931, 1481
PsyI GACNNNGTC 1 cut(s) 820
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 5 cut(s) 491, 759, 1096, 1375, 1411
RsaNI GTAC 5 cut(s) 490, 758, 1095, 1374, 1410
RseI CAYNNNNRTG 3 cut(s) 320, 675, 1062
SacI GAGCTC 1 cut(s) 189
SalI GTCGAC 1 cut(s) 503
SaqAI TTAA 2 cut(s) 1107, 1356
SatI GCNGC 3 cut(s) 120, 153, 1075
Sau3AI GATC 5 cut(s) 448, 611, 787, 1020, 1198
Sau96I GGNCC 5 cut(s) 34, 728, 764, 1221, 1507
ScaI AGTACT 1 cut(s) 759
SchI GAGTC 2 cut(s) 464, 619
ScrFI CCNGG 2 cut(s) 108, 1518
SduI GDGCHC 3 cut(s) 189, 1341, 1558
SfaNI GCATC 3 cut(s) 54, 553, 946
SfcI CTRYAG 3 cut(s) 882, 906, 1072
SinI GGWCC 4 cut(s) 728, 764, 1221, 1507
SmiMI CAYNNNNRTG 3 cut(s) 320, 675, 1062
SmlI CTYRAG 1 cut(s) 1136
SmoI CTYRAG 1 cut(s) 1136
SphI GCATGC 1 cut(s) 674
SspI AATATT 1 cut(s) 1036
SspMI CTAG 3 cut(s) 465, 761, 1322
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 106, 1516
StyI CCWWGG 1 cut(s) 1282
TaaI ACNGT 6 cut(s) 160, 221, 433, 559, 1483, 1525
TaqI TCGA 2 cut(s) 504, 1269
TaqII GACCGA 2 cut(s) 189, 1116
TatI WGTACW 2 cut(s) 757, 1409
TfiI GAWTC 4 cut(s) 642, 848, 856, 919
Tru1I TTAA 2 cut(s) 1107, 1356
Tru9I TTAA 2 cut(s) 1107, 1356
TseFI GTSAC 4 cut(s) 84, 160, 1415, 1477
TseI GCWGC 3 cut(s) 119, 152, 1074
Tsp45I GTSAC 4 cut(s) 84, 160, 1415, 1477
TspDTI ATGAA 5 cut(s) 92, 270, 396, 869, 1046
Tth111I GACNNNGTC 1 cut(s) 820
VpaK11BI GGWCC 4 cut(s) 728, 764, 1221, 1507
XagI CCTNNNNNAGG 1 cut(s) 1515
XapI RAATTY 4 cut(s) 304, 687, 1265, 1358
XceI RCATGY 4 cut(s) 92, 587, 670, 674
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 3 cut(s) 465, 761, 1322
ZrmI AGTACT 1 cut(s) 759
Zsp2I ATGCAT 2 cut(s) 585, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.