Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0001965

Physical Location & Seq

Genomic Coordinates Reverse (-)

47326150 .. 47336874

Gene Structure 10725 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014106

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2382 bp

ATGGGAGCTAGATCAGCTTTCAACTGGTTTCTGCTTTACATGGTTTTCTCGTCTCCATGTCTTCAGCTGACACAGTGCAATGTTACCTATGATAGGGAGGCTCTTCTCATCAATGGACAGAGGATCGGAGAATTCTCTTTTCTGGTTCCATACACTATCCTAGGAATGTGGGAAGATCTTATACTGAAGGCAAAAGATGGTGGCTTGGATGTCATAGACACCTATGTGTTTTGGAATCTTCATGAGCCTTCTCCGGGCAATTACACTTTTGAGGAGAGGTATGATTTGGTTCGGTTCATAAAGCTGGTTCAAACAGCAGGGCTCTATGTGCTTCTTCGCATTGGACCTTATATATGTGCAGAATGGAATTTTGGGGGGTTTCCAGTTTGGCTCAAGTATGTTCCAGGCATTAGCTTCAGGACAAATAATGAGCCTTTCAAGGCAGCAATGCAGACGTTTACTCAGAAAATTGTCCAATTGATGAAGGATGAGGAATTATTTGAGTCTCAAGGTGCCGGTCATGCATACATGACATGGGCTGCAGAGATGGCTGTTGGCATGGATACTGGGGTCCCATGGGTCATGTGCAAGGAAGATGATGCCCCAGACCCTGTGATAAACACTTGCAATGGGTTTTATTGTGACAATTTTTCTCCAAACAAGCCATACAAGCCCACGATCTGGACTGAGGCTTGGACTGGCTGGTTTACACAATTTGGTGGCCCAAATTACAAGCGACCTGTTGAAGATTTGGCATTTGCAGTTGCTCGATTTGTTCAGAACGGAGGCTCCTTCATAAACTATTACATGTACCATGGAGGAACTAACTTTGGAAGAACTGCTGGAGGCCCTTTTATTATTACCAGCTATGACTACGATGCCCCCATTGATGAATATGGCTTGATCAGGCAACCCAAGTATGGTCACTTAATGGAACTCCATAAAGCTGTTAAGTTATGTGAGAAAGCTCTGCTAAATGCTGATCCGACTGTTACATCCTTAGGGAGATATCAACAGTCACATGTATTCTCTTCAAAGCCGGGAGGTTGTGCAGCATTTCTCTCAAACTACAATGCAAACTCAGCTGCAACGGTGACTTTCAATGACCGAAACTATGTGCTCCCTGCTTGGTCTATCAGCGTCCTTCCAGACTGCGAAAATGTTGTATTCAACACTGCCAAAGTGGGAGTTCAGACATCCCAAAAACAATTTTTTCCCACTAACTCTGAGTTGTGGTCATGGGAAACCTTCAGTGAAGACACTGCTTCATGGACTGGTGATACAACTCTCACAGTCATTGGTTTCTTAGATCAGTTGAACATCACTAGAGACTCCAGTGATTATCTATGGTACACCACCAGTGTCGACATAAGTCCATCTGAATCATTTCTGCGTGGAGGTAAGCATCCAATTCTCACTGTGCAGTCTGCTGGGAATGCTATGCATGTCTTTATCAATGACCAGCTCTCAGGATCAACTTATGGGACTCTACAAGACCAGAGATTCACTTTTACAGGAGATGTAAACAAGCATGCTGGTGTGAATAAAATTTCACTTCTAAGTGTAGCTATTGGATTACCGGTTAGAAGCAGAGATTTATCAGGGCAAACATGGTCATACAAGGTTGGGCTAAAAGGAGAATCCATGAATCTCGGTTCTCCAACTTCCACCTCTGCAGTGAATTGGACGAGGGTGTCTATAGGGACACAGAATCAACAGCCACTGACTTGGTATAAGGCCAATTTTGATGCACCTGAAGGGGATGAGCCTTTGGCTTTGGATATGAGGAGCATGGGGAAGGGTCAAGTATGGATCAATGGGGAGAGTATTGGAAGATACTGGATTATTCATGCTAATGGTAACTGCAGCGCGTGCAGTTATTCTGGTACATTCAGGATTAACAAGTGCCAATTTGGTTGTCTCCAACCAACTCAACAATGGTATCATGTTCCTCGGTCTTGGCTCAAGCCATCTCAAAATCTGTTGGTTGTTTTTGAGGAGCTTGCTGGCGATGTTTCAAAGATTGACCTTGTGAAAACATTCGTGAGGACCATCTGTGCCAAGATTTCTAAAAGTTACCCACTCGTTAGGGAATTTGACACCAAGAGCCAAGGCAAATTGAAAGAGCAAAAGAAGCCTAAACTTAGCCTAGAGTGTAAAAAGCCTAAACTTAGCCTAGAGTGTAAAAATGGGCACTCTATTTCTGCTATTAAATTTTCAAGCTTCGGTACTCCATCCGAAAGCTGTGGAAACTATAAGCATTGTACCTGTCACGCTCCAAACTCTACCCCTGTGCTAAATAAGGTACCCTCGCACTGCTACTGTTTTGCTTCAGTTCAGGGTCAAAGTATTTATCCAAATTCAAAATTAATAAAATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0009505 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

794

Amino Acids

88.78

Weight (kDa)

7.72

Isoelectric Point (pI)

36.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 171	3.2e-49	Glycosyl hydrolases family 35
Glyco_hydro_35	PF01301	173 - 316	1.5e-44	Glycosyl hydrolases family 35
GHD	PF17834	339 - 395	7.5e-24	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	448 - 503	4.4e-06	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	575 - 660	5.7e-28	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36360 AT4G36360
fragaria_vesca	FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_2g09820 FvH4_2g09820
malus_domestica	MD02G1137500.v1.1 MD10G1067800.v1.1 MD15G1251100.v1.1
prunus_persica	Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.8G089500_v2.0.a1 Prupe.8G089500_v2.0.a1
pyrus_communis	pycom02g10880 pycom10g05360 pycom15g22140
rosa_chinensis	RchiOBHm_Chr2g0100171 RchiOBHm_Chr6g0266171
rosa_laevigata	RLG00000014106 RLG00000014109 RLG00000016943
rosa_multiflora	Rmu_sc0002547.1_g000001
rosa_roxburghii	Rroxscaffold_174G00435340 Rroxscaffold_2G00142500 Rroxscaffold_7G00201780 Rroxscaffold_7G00201840
rosa_rugosa	Rorug02G0086200 Rorug06G0024500 Rorug06G0024900 Rorug06G0025000
rosa_samantha	Rh2AG135300 Rh2CG140500 Rh2DG140700 Rh6BG145900 Rh6CG142300 Rh6DG130400
rosa_wichuraiana	Rw2G010560 Rw6G012540 Rw6G012580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1693
Acc65I	GGTACC	1 cut(s)	2305
AccB1I	GGYRCC	2 cut(s)	512, 2305
AccB7I	CCANNNNNTGG	1 cut(s)	681
AccI	GTMKAC	1 cut(s)	1365
AccII	CGCG	1 cut(s)	1871
AclWI	GGATC	4 cut(s)	131, 977, 1480, 1820
AcsI	RAATTY	6 cut(s)	131, 367, 1548, 2093, 2213, 2359
AcuI	CTGAAG	6 cut(s)	47, 206, 400, 1234, 1776, 2316
AfaI	GTAC	6 cut(s)	812, 1352, 1888, 2230, 2266, 2307
AfiI	CCNNNNNNNGG	4 cut(s)	93, 254, 681, 920
AflIII	ACRYGT	2 cut(s)	807, 1021
AgeI	ACCGGT	1 cut(s)	1579
AjnI	CCWGG	1 cut(s)	403
AjuI	GAANNNNNNNTTGG	2 cut(s)	354, 386
AleI	CACNNNNGTG	1 cut(s)	224
AloI	GAACNNNNNNTCC	2 cut(s)	773, 805
Alw21I	GWGCWC	1 cut(s)	1122
Alw26I	GTCTC	4 cut(s)	57, 510, 1323, 1925
AlwI	GGATC	4 cut(s)	131, 977, 1480, 1820
AlwNI	CAGNNNCTG	2 cut(s)	611, 1723
AoxI	GGCC	3 cut(s)	721, 847, 1737
ApeKI	GCWGC	5 cut(s)	443, 539, 1052, 1085, 1866
ApoI	RAATTY	6 cut(s)	131, 367, 1548, 2093, 2213, 2359
AseI	ATTAAT	1 cut(s)	2369
AsiGI	ACCGGT	1 cut(s)	1579
Asp700I	GAANNNNTTC	2 cut(s)	1386, 2039
Asp718I	GGTACC	1 cut(s)	2305
AspA2I	CCTAGG	1 cut(s)	160
AspLEI	GCGC	1 cut(s)	1871
AspS9I	GGNCC	5 cut(s)	344, 571, 722, 848, 2049
AsuC2I	CCSGG	2 cut(s)	255, 1041
AsuHPI	GGTGA	2 cut(s)	1105, 1289
AvaII	GGWCC	3 cut(s)	344, 571, 2049
AvrII	CCTAGG	1 cut(s)	160
AxyI	CCTNAGG	1 cut(s)	1000
BaeGI	GKGCMC	1 cut(s)	2196
BaeI	ACNNNNGTAYC	4 cut(s)	555, 588, 1925, 1958
BanI	GGYRCC	2 cut(s)	512, 2305
BanII	GRGCYC	1 cut(s)	324
BarI	GAAGNNNNNNTAC	2 cut(s)	165, 197
BbsI	GAAGAC	2 cut(s)	53, 1263
Bbv12I	GWGCWC	1 cut(s)	1122
BbvI	GCAGC	5 cut(s)	455, 526, 1064, 1072, 1878
BccI	CCATC	6 cut(s)	191, 541, 1384, 1978, 2060, 2242
BciT130I	CCWGG	1 cut(s)	405
BciVI	GTATCC	1 cut(s)	556
BclI	TGATCA	1 cut(s)	903
BcnI	CCSGG	2 cut(s)	255, 1041
BcoDI	GTCTC	4 cut(s)	57, 510, 1323, 1925
BfaI	CTAG	5 cut(s)	9, 161, 1326, 2150, 2177
BfmI	CTRYAG	4 cut(s)	540, 1674, 1698, 1864
BfuI	GTATCC	1 cut(s)	556
BglII	AGATCT	1 cut(s)	175
BisI	GCNGC	5 cut(s)	444, 540, 1053, 1086, 1867
BlnI	CCTAGG	1 cut(s)	160
BlsI	GCNGC	5 cut(s)	445, 541, 1054, 1087, 1868
Bme1390I	CCNGG	3 cut(s)	255, 405, 1041
Bme18I	GGWCC	3 cut(s)	344, 571, 2049
BmgT120I	GGNCC	5 cut(s)	344, 571, 722, 848, 2049
BmiI	GGNNCC	6 cut(s)	147, 514, 572, 573, 790, 2307
BmrFI	CCNGG	3 cut(s)	255, 405, 1041
BmrI	ACTGGG	1 cut(s)	576
BmsI	GCATC	4 cut(s)	589, 868, 1414, 1738
BmuI	ACTGGG	1 cut(s)	576
BoxI	GACNNNNGTC	1 cut(s)	1371
BpiI	GAAGAC	2 cut(s)	53, 1263
BpmI	CTGGAG	2 cut(s)	864, 1318
BpuEI	CTTGAG	3 cut(s)	377, 492, 1949
BpuMI	CCSGG	2 cut(s)	255, 1041
BsaJI	CCNNGG	5 cut(s)	160, 575, 814, 1952, 2110
BsaWI	WCCGGW	1 cut(s)	1579
BsaXI	ACNNNNNCTCC	2 cut(s)	773, 803
Bsc4I	CCNNNNNNNGG	4 cut(s)	93, 254, 681, 920
Bse118I	RCCGGY	2 cut(s)	515, 1579
Bse1I	ACTGG	8 cut(s)	29, 383, 571, 703, 1279, 1335, 1359, 1844
Bse21I	CCTNAGG	1 cut(s)	1000
Bse3DI	GCAATG	3 cut(s)	85, 453, 634
BseBI	CCWGG	1 cut(s)	405
BseDI	CCNNGG	5 cut(s)	160, 575, 814, 1952, 2110
BseGI	GGATG	7 cut(s)	214, 493, 995, 1196, 1405, 1768, 2234
BseLI	CCNNNNNNNGG	4 cut(s)	93, 254, 681, 920
BseMI	GCAATG	3 cut(s)	85, 453, 634
BseMII	CTCAG	5 cut(s)	476, 678, 1095, 1218, 1482
BseNI	ACTGG	8 cut(s)	29, 383, 571, 703, 1279, 1335, 1359, 1844
BseRI	GAGGAG	3 cut(s)	287, 1801, 2012
BseSI	GKGCMC	1 cut(s)	2196
BseXI	GCAGC	5 cut(s)	455, 526, 1064, 1072, 1878
BseYI	CCCAGC	1 cut(s)	1430
BsgI	GTGCAG	4 cut(s)	378, 1071, 1442, 1894
Bsh1236I	CGCG	1 cut(s)	1871
BshFI	GGCC	3 cut(s)	723, 849, 1739
BshNI	GGYRCC	2 cut(s)	512, 2305
BshTI	ACCGGT	1 cut(s)	1579
BsiHKAI	GWGCWC	1 cut(s)	1122
BsiSI	CCGG	4 cut(s)	254, 516, 1040, 1580
BslFI	GGGAC	3 cut(s)	557, 1498, 1717
BslI	CCNNNNNNNGG	4 cut(s)	93, 254, 681, 920
BsmAI	GTCTC	4 cut(s)	57, 510, 1323, 1925
BsmBI	CGTCTC	1 cut(s)	57
BsmFI	GGGAC	3 cut(s)	557, 1498, 1717
BsmI	GAATGC	1 cut(s)	1441
BsnI	GGCC	3 cut(s)	723, 849, 1739
Bsp1286I	GDGCHC	3 cut(s)	324, 1122, 2196
Bsp143I	GATC	9 cut(s)	11, 123, 175, 678, 903, 982, 1309, 1472, 1812
Bsp19I	CCATGG	2 cut(s)	575, 814
BspANI	GGCC	3 cut(s)	723, 849, 1739
BspCNI	CTCAG	5 cut(s)	475, 679, 1094, 1219, 1481
BspFNI	CGCG	1 cut(s)	1871
BspHI	TCATGA	1 cut(s)	241
BspLI	GGNNCC	6 cut(s)	147, 514, 572, 573, 790, 2307
BspMAI	CTGCAG	3 cut(s)	544, 1678, 1868
BspPI	GGATC	4 cut(s)	131, 977, 1480, 1820
BspQI	GCTCTTC	1 cut(s)	108
BspT107I	GGYRCC	2 cut(s)	512, 2305
BsrDI	GCAATG	3 cut(s)	85, 453, 634
BsrFI	RCCGGY	2 cut(s)	515, 1579
BsrI	ACTGG	8 cut(s)	29, 383, 571, 703, 1279, 1335, 1359, 1844
BssAI	RCCGGY	2 cut(s)	515, 1579
BssECI	CCNNGG	5 cut(s)	160, 575, 814, 1952, 2110
BssMI	GATC	9 cut(s)	11, 123, 175, 678, 903, 982, 1309, 1472, 1812
BssT1I	CCWWGG	4 cut(s)	160, 575, 814, 2110
Bst2UI	CCWGG	1 cut(s)	405
Bst4CI	ACNGT	7 cut(s)	75, 991, 1017, 1093, 1294, 1420, 2324
Bst6I	CTCTTC	2 cut(s)	108, 1036
BstAPI	GCANNNNNTGC	1 cut(s)	1872
BstC8I	GCNNGC	4 cut(s)	1533, 1873, 2004, 2008
BstDSI	CCRYGG	2 cut(s)	575, 814
BstENI	CCTNNNNNAGG	1 cut(s)	91
BstF5I	GGATG	7 cut(s)	214, 493, 995, 1196, 1405, 1768, 2234
BstFNI	CGCG	1 cut(s)	1871
BstHHI	GCGC	1 cut(s)	1871
BstKTI	GATC	9 cut(s)	14, 126, 178, 681, 906, 985, 1312, 1475, 1815
BstMAI	GTCTC	4 cut(s)	57, 510, 1323, 1925
BstMBI	GATC	9 cut(s)	11, 123, 175, 678, 903, 982, 1309, 1472, 1812
BstMWI	GCNNNNNNNGC	9 cut(s)	14, 328, 521, 548, 670, 1082, 1436, 1872, 2134
BstNI	CCWGG	1 cut(s)	405
BstNSI	RCATGY	4 cut(s)	811, 1025, 1448, 1535
BstPAI	GACNNNNGTC	1 cut(s)	1371
BstSCI	CCNGG	3 cut(s)	253, 403, 1039
BstSFI	CTRYAG	4 cut(s)	540, 1674, 1698, 1864
BstSLI	GKGCMC	1 cut(s)	2196
BstUI	CGCG	1 cut(s)	1871
BstV1I	GCAGC	5 cut(s)	455, 526, 1064, 1072, 1878
BstV2I	GAAGAC	2 cut(s)	53, 1263
BstX2I	RGATCY	1 cut(s)	175
BstXI	CCANNNNNNTGG	1 cut(s)	1728
BstYI	RGATCY	1 cut(s)	175
Bsu36I	CCTNAGG	1 cut(s)	1000
BsuI	GTATCC	1 cut(s)	556
BsuRI	GGCC	3 cut(s)	723, 849, 1739
BtgI	CCRYGG	2 cut(s)	575, 814
BtgZI	GCGATG	1 cut(s)	2025
BtsCI	GGATG	7 cut(s)	214, 493, 995, 1196, 1405, 1768, 2234
BtsI	GCAGTG	4 cut(s)	1173, 1260, 1683, 2314
Cac8I	GCNNGC	4 cut(s)	1533, 1873, 2004, 2008
CaiI	CAGNNNCTG	2 cut(s)	611, 1723
CciI	TCATGA	1 cut(s)	241
CfoI	GCGC	1 cut(s)	1871
Cfr10I	RCCGGY	2 cut(s)	515, 1579
Cfr13I	GGNCC	5 cut(s)	344, 571, 722, 848, 2049
CseI	GACGC	1 cut(s)	1129
Csp6I	GTAC	6 cut(s)	811, 1351, 1887, 2229, 2265, 2306
CspAI	ACCGGT	1 cut(s)	1579
CviQI	GTAC	6 cut(s)	811, 1351, 1887, 2229, 2265, 2306
DpnI	GATC	9 cut(s)	13, 125, 177, 680, 905, 984, 1311, 1474, 1814
DpnII	GATC	9 cut(s)	11, 123, 175, 678, 903, 982, 1309, 1472, 1812
DrdI	GACNNNNNNGTC	1 cut(s)	1693
DseDI	GACNNNNNNGTC	1 cut(s)	1693
Eam1104I	CTCTTC	2 cut(s)	108, 1036
EarI	CTCTTC	2 cut(s)	108, 1036
Eco130I	CCWWGG	4 cut(s)	160, 575, 814, 2110
Eco24I	GRGCYC	1 cut(s)	324
Eco32I	GATATC	1 cut(s)	1010
Eco47I	GGWCC	3 cut(s)	344, 571, 2049
Eco57I	CTGAAG	6 cut(s)	47, 206, 400, 1234, 1776, 2316
Eco81I	CCTNAGG	1 cut(s)	1000
EcoNI	CCTNNNNNAGG	1 cut(s)	91
EcoO109I	RGGNCCY	2 cut(s)	571, 848
EcoRI	GAATTC	1 cut(s)	131
EcoRII	CCWGG	1 cut(s)	403
EcoRV	GATATC	1 cut(s)	1010
EcoT14I	CCWWGG	4 cut(s)	160, 575, 814, 2110
EcoT22I	ATGCAT	2 cut(s)	526, 1446
EcoT38I	GRGCYC	1 cut(s)	324
ErhI	CCWWGG	4 cut(s)	160, 575, 814, 2110
Esp3I	CGTCTC	1 cut(s)	57
FaqI	GGGAC	3 cut(s)	557, 1498, 1717
FbaI	TGATCA	1 cut(s)	903
FblI	GTMKAC	1 cut(s)	1365
Fnu4HI	GCNGC	5 cut(s)	444, 540, 1053, 1086, 1867
FokI	GGATG	7 cut(s)	221, 500, 982, 1183, 1392, 1775, 2221
FriOI	GRGCYC	1 cut(s)	324
Fsp4HI	GCNGC	5 cut(s)	444, 540, 1053, 1086, 1867
FspBI	CTAG	5 cut(s)	9, 161, 1326, 2150, 2177
GlaI	GCGC	1 cut(s)	1870
GluI	GCNGC	5 cut(s)	444, 540, 1053, 1086, 1867
GsaI	CCCAGC	1 cut(s)	1434
GsuI	CTGGAG	2 cut(s)	864, 1318
HaeIII	GGCC	3 cut(s)	723, 849, 1739
HapII	CCGG	4 cut(s)	254, 516, 1040, 1580
HgaI	GACGC	1 cut(s)	1129
HhaI	GCGC	1 cut(s)	1871
Hin6I	GCGC	1 cut(s)	1869
HinP1I	GCGC	1 cut(s)	1869
HincII	GTYRAC	1 cut(s)	1366
HindII	GTYRAC	1 cut(s)	1366
HindIII	AAGCTT	1 cut(s)	2221
HinfI	GANTC	9 cut(s)	235, 503, 1331, 1382, 1486, 1503, 1640, 1648, 1711
HpaII	CCGG	4 cut(s)	254, 516, 1040, 1580
HphI	GGTGA	2 cut(s)	1105, 1289
Hpy166II	GTNNAC	5 cut(s)	459, 708, 1353, 1366, 1525
Hpy188I	TCNGA	8 cut(s)	128, 465, 780, 987, 1194, 1228, 1381, 2239
Hpy188III	TCNNGA	7 cut(s)	242, 418, 682, 1148, 1470, 1894, 2044
Hpy8I	GTNNAC	5 cut(s)	459, 708, 1353, 1366, 1525
HpyAV	CCTTC	8 cut(s)	181, 258, 478, 802, 1154, 1258, 1751, 1792
HpyCH4III	ACNGT	7 cut(s)	75, 991, 1017, 1093, 1294, 1420, 2324
HpyCH4IV	ACGT	1 cut(s)	455
HpyF10VI	GCNNNNNNNGC	9 cut(s)	14, 328, 521, 548, 670, 1082, 1436, 1872, 2134
HpySE526I	ACGT	1 cut(s)	455
HspAI	GCGC	1 cut(s)	1869
KflI	GGGWCCC	1 cut(s)	571
KpnI	GGTACC	1 cut(s)	2309
Ksp22I	TGATCA	1 cut(s)	903
Kzo9I	GATC	9 cut(s)	11, 123, 175, 678, 903, 982, 1309, 1472, 1812
LguI	GCTCTTC	1 cut(s)	108
LmnI	GCTCC	6 cut(s)	5, 794, 1125, 1788, 1999, 2281
Lsp1109I	GCAGC	5 cut(s)	455, 526, 1064, 1072, 1878
LweI	GCATC	4 cut(s)	589, 868, 1414, 1738
MaeI	CTAG	5 cut(s)	9, 161, 1326, 2150, 2177
MaeII	ACGT	1 cut(s)	455
MaeIII	GTNAC	9 cut(s)	82, 641, 923, 991, 1017, 1093, 1859, 2075, 2270
MalI	GATC	9 cut(s)	13, 125, 177, 680, 905, 984, 1311, 1474, 1814
MboI	GATC	9 cut(s)	11, 123, 175, 678, 903, 982, 1309, 1472, 1812
MfeI	CAATTG	1 cut(s)	476
MflI	RGATCY	1 cut(s)	175
MhlI	GDGCHC	3 cut(s)	324, 1122, 2196
MlyI	GAGTC	3 cut(s)	512, 1325, 1480
MmeI	TCCRAC	3 cut(s)	1010, 1685, 1948
Mph1103I	ATGCAT	2 cut(s)	526, 1446
MroXI	GAANNNNTTC	2 cut(s)	1386, 2039
MseI	TTAA	5 cut(s)	929, 951, 1899, 2211, 2369
MslI	CAYNNNNRTG	3 cut(s)	224, 1536, 1854
MspA1I	CMGCKG	2 cut(s)	67, 1085
MspI	CCGG	4 cut(s)	254, 516, 1040, 1580
MspR9I	CCNGG	3 cut(s)	255, 405, 1041
MunI	CAATTG	1 cut(s)	476
Mva1269I	GAATGC	1 cut(s)	1441
MvaI	CCWGG	1 cut(s)	405
MvnI	CGCG	1 cut(s)	1871
MwoI	GCNNNNNNNGC	9 cut(s)	14, 328, 521, 548, 670, 1082, 1436, 1872, 2134
NciI	CCSGG	2 cut(s)	255, 1041
NcoI	CCATGG	2 cut(s)	575, 814
NdeII	GATC	9 cut(s)	11, 123, 175, 678, 903, 982, 1309, 1472, 1812
NlaIV	GGNNCC	6 cut(s)	147, 514, 572, 573, 790, 2307
NmuCI	GTSAC	5 cut(s)	641, 923, 1017, 1093, 2270
NsiI	ATGCAT	2 cut(s)	526, 1446
NspI	RCATGY	4 cut(s)	811, 1025, 1448, 1535
OliI	CACNNNNGTG	1 cut(s)	224
PaeI	GCATGC	1 cut(s)	1535
PagI	TCATGA	1 cut(s)	241
PciI	ACATGT	2 cut(s)	807, 1021
PciSI	GCTCTTC	1 cut(s)	108
PctI	GAATGC	1 cut(s)	1441
PdmI	GAANNNNTTC	2 cut(s)	1386, 2039
PfeI	GAWTC	6 cut(s)	235, 1382, 1503, 1640, 1648, 1711
PflMI	CCANNNNNTGG	1 cut(s)	681
PinAI	ACCGGT	1 cut(s)	1579
PkrI	GCNGC	5 cut(s)	445, 541, 1054, 1087, 1868
PleI	GAGTC	3 cut(s)	511, 1325, 1480
PpsI	GAGTC	3 cut(s)	511, 1325, 1480
PpuMI	RGGWCCY	1 cut(s)	571
PscI	ACATGT	2 cut(s)	807, 1021
PshAI	GACNNNNGTC	1 cut(s)	1371
PshBI	ATTAAT	1 cut(s)	2369
Psp5II	RGGWCCY	1 cut(s)	571
Psp6I	CCWGG	1 cut(s)	403
PspFI	CCCAGC	1 cut(s)	1430
PspGI	CCWGG	1 cut(s)	403
PspN4I	GGNNCC	6 cut(s)	147, 514, 572, 573, 790, 2307
PspPI	GGNCC	5 cut(s)	344, 571, 722, 848, 2049
PspPPI	RGGWCCY	1 cut(s)	571
PstI	CTGCAG	3 cut(s)	544, 1678, 1868
PstNI	CAGNNNCTG	2 cut(s)	611, 1723
PsuI	RGATCY	1 cut(s)	175
PvuII	CAGCTG	2 cut(s)	67, 1085
RsaI	GTAC	6 cut(s)	812, 1352, 1888, 2230, 2266, 2307
RsaNI	GTAC	6 cut(s)	811, 1351, 1887, 2229, 2265, 2306
RseI	CAYNNNNRTG	3 cut(s)	224, 1536, 1854
SalI	GTCGAC	1 cut(s)	1364
SapI	GCTCTTC	1 cut(s)	108
SaqAI	TTAA	5 cut(s)	929, 951, 1899, 2211, 2369
SatI	GCNGC	5 cut(s)	444, 540, 1053, 1086, 1867
Sau3AI	GATC	9 cut(s)	11, 123, 175, 678, 903, 982, 1309, 1472, 1812
Sau96I	GGNCC	5 cut(s)	344, 571, 722, 848, 2049
SchI	GAGTC	3 cut(s)	512, 1325, 1480
ScrFI	CCNGG	3 cut(s)	255, 405, 1041
SduI	GDGCHC	3 cut(s)	324, 1122, 2196
SfaNI	GCATC	4 cut(s)	589, 868, 1414, 1738
SfcI	CTRYAG	4 cut(s)	540, 1674, 1698, 1864
SinI	GGWCC	3 cut(s)	344, 571, 2049
SmiMI	CAYNNNNRTG	3 cut(s)	224, 1536, 1854
SmlI	CTYRAG	3 cut(s)	392, 507, 1964
SmoI	CTYRAG	3 cut(s)	392, 507, 1964
SphI	GCATGC	1 cut(s)	1535
SspMI	CTAG	5 cut(s)	9, 161, 1326, 2150, 2177
StyD4I	CCNGG	3 cut(s)	253, 403, 1039
StyI	CCWWGG	4 cut(s)	160, 575, 814, 2110
TaaI	ACNGT	7 cut(s)	75, 991, 1017, 1093, 1294, 1420, 2324
TaiI	ACGT	1 cut(s)	458
TaqI	TCGA	2 cut(s)	769, 1365
TaqII	GACCGA	2 cut(s)	1122, 1944
TfiI	GAWTC	6 cut(s)	235, 1382, 1503, 1640, 1648, 1711
Tru1I	TTAA	5 cut(s)	929, 951, 1899, 2211, 2369
Tru9I	TTAA	5 cut(s)	929, 951, 1899, 2211, 2369
TseFI	GTSAC	5 cut(s)	641, 923, 1017, 1093, 2270
TseI	GCWGC	5 cut(s)	443, 539, 1052, 1085, 1866
Tsp45I	GTSAC	5 cut(s)	641, 923, 1017, 1093, 2270
TspDTI	ATGAA	8 cut(s)	230, 286, 497, 784, 906, 1257, 1661, 1838
TspGWI	ACGGA	1 cut(s)	798
Van91I	CCANNNNNTGG	1 cut(s)	681
VpaK11BI	GGWCC	3 cut(s)	344, 571, 2049
VspI	ATTAAT	1 cut(s)	2369
XagI	CCTNNNNNAGG	1 cut(s)	91
XapI	RAATTY	6 cut(s)	131, 367, 1548, 2093, 2213, 2359
XceI	RCATGY	4 cut(s)	811, 1025, 1448, 1535
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1935
XmaJI	CCTAGG	1 cut(s)	160
XmiI	GTMKAC	1 cut(s)	1365
XmnI	GAANNNNTTC	2 cut(s)	1386, 2039
XspI	CTAG	5 cut(s)	9, 161, 1326, 2150, 2177
Zsp2I	ATGCAT	2 cut(s)	526, 1446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.