Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0001965

Physical Location & Seq

Genomic Coordinates Forward (+)

11804896 .. 11810597

Gene Structure 5702 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG140700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGTGGGAGGATCTGATACAGAAGGCAAAAGATGGAGGTCTCGATGTGGTTGAAACCTATGTGTTCTGGAATGGCCATGAACCTACCCCTGGCAATTTCAATTTTGAAGGGAGGTATGATCTGGTGAGATTCCTGAAGACCATACAGAAAGCAGGGCTCTATGCTCATCTTCGCATTGGGCCTTATGTTTGTGCTGAGTGGAATTTTGGGGGTTTTCCTGTTTGGCTGAAATATGTTCCGGGCATCAGTTTTAGAACAGACAATGAGCCTTTCAAGAGGGCAATGCAAGGGTTTACTGAGAAGATTGTGGGACTGATGAAGAGTGAAAAATTGTTCGAGTCCCAGGGAGGCCCCATTATTCTATCACAGATTGAGAATGAGTACGGAGTACAGAGCAAGTTATTTGGAGCTGCTGGACATAATTACATGACCTGGGCAGCAGAGATGGCTGTTGGGTTGGGAACTGGGGTCCCATGGGTGATGTGCAAGGAAGAAGATGCTCCAGATCCAGTGATAAACACATGTAATGGTTTCTACTGTGATTCTTTCTCTCCCAACAAACCATACAAGCCTACGATTTGGACAGAGGCCTGGAGTGGCTGGTTTACGGAGTTTGGAGGTCCAATTCACCAACGACCAGTTCAAGATTTAGCATATGCAGTGGCCAGATTCATACAGAACGGAGGATCATTTGTTAATTATTACATGTACCATGGAGGAACCAACTTTGGACGCACAGCAGGGGGACCTTTCGTCACTACCAGCTACGACTATGATGCTCCATTAGATGAATATGGTTTAATCAGGCAGCCAAAGTATGGTCATTTGAAGGAGCTTCATAAGTCTATTAAGATGTGTGAACGGGCTTTAGTTTCAGCTGATCCAATCATCACTTCACTTGGGGACTTTCAACAGGCTCATGTGTATACTTCAGAATCAGGAGATTGTGCAGCATTTCTTTCGAACCACAATTCCAAGTCAGCTGCAAGGGTGATGTTTAATAACATGCACTATAACCTGCCTCCATGGTCAATCAGTATCCTTCCTGATTGCAGAAATGTAGTCTTTAACACTGCAAAGGTTGGAGTTCAAACATCACAAATGCAAATGTTGCCAACAAATGTTGAGACGCTATCATGGGAGACATATGATGAAGATCTTACATCTCTAGATGACAGCTCAACGTTCACTGCTCCAGGTCTGTTGGAACAGATAAATGTTACAAGGGATACTACTGATTACCTGTGGTACATAACTAGCGTTGACATTGGTTCATCTGAATCCTTCCTGCGTGGAGGGGAACTCCCAACTCTTATTGTTCAGTCAACAGGTCATGCTGTGCACATCTTCATTAATGGACAGCTTTCAGGTTCTGCCTTTGGAACAAGGGAGAATAGGAAATTCACATATACCGGCAAAGTCAATCTACGTGCAGGAACCAACAAAATTGCGCTGCTCAGTGTTGCTGTTGGATTGCCAAATGTGGGCGGACACTACGAAGCATGGAATACAGGAATCCTTGGCCCAGTTGCACTTCATGGACTTGACCAGGGAAAATGGGACTTGTCTTGGCAGAAATGGACCTACCAGGTTGGATTGAAAGGAGAAGCCATGAGCCTTGTATCTCCAAATAGTATTTCCTCTGTTGAATGGCTGCAAGCATCATTAGCTGCCCAAAAACAGCAGCCATTGACTTGGCATAAGAGCTTTTTCAATGCACCTGACGGAGATGAGCCATTGGCTTTGGATATGGAGGGAATGGGAAAAGGCCAGATATGGATTAACGGGCAGAGTATTGGAAGATACTGGACTGCATTTGCTAATGGTGATTGCAATGGGTGCAGTTATGCTGGAGGTTTCAGACCTACAAAGTGTCAAACAGGTTGTGGTCAACCCACACAAAGATACTATCATGTGCCTCGGTCTTGGTTAAAGCCAATGCAAAATCTGTTGGTAATTTTTGAGGAACTTGGAGGCGATCCTTCAAGAATTTCTATTGTAAAGAGATCTGTGAGCACTGTCTGTGCTGACGTTGCTGAGTTCCATCCAACAATAAAGAACTGGCACATTGAAAGCTATGGAAAGGTAGAAGAATTTCACAGCCCCAAGGTTCACTTGAAGTGTAACCCAGGCCAGTCCATCTCTTCCATTAAATTCGCTAGTTTTGGCACACCTTTCGGGACCTGTGGAACTTACCAGCAAGGAAGTTGCCATGCTTCGACATCATATTCTGTACTAGAGAAGATGTGCATAGGGAAGCAAAGATGTGCAGTCACCATATCCAACAGTAACTTTGGAGATCCATGTCCGAAGGTATTGAAGCGCCTCTCAGTTGAAGCAGTTTGTGCTCCTACAACTTCAACAACTGCACAACCCAATTGGAGGGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0009505 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

796

Amino Acids

88.17

Weight (kDa)

6.41

Isoelectric Point (pI)

36.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	1 - 282	6.5e-110	Glycosyl hydrolases family 35
GHD	PF17834	305 - 361	2.1e-26	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	414 - 481	2.2e-07	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	564 - 649	1.3e-24	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	706 - 782	5.9e-21	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36360 AT4G36360
fragaria_vesca	FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_2g09820 FvH4_2g09820
malus_domestica	MD02G1137500.v1.1 MD10G1067800.v1.1 MD15G1251100.v1.1
prunus_persica	Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.8G089500_v2.0.a1 Prupe.8G089500_v2.0.a1
pyrus_communis	pycom02g10880 pycom10g05360 pycom15g22140
rosa_chinensis	RchiOBHm_Chr2g0100171 RchiOBHm_Chr6g0266171
rosa_laevigata	RLG00000014106 RLG00000014109 RLG00000016943
rosa_multiflora	Rmu_sc0002547.1_g000001
rosa_roxburghii	Rroxscaffold_174G00435340 Rroxscaffold_2G00142500 Rroxscaffold_7G00201780 Rroxscaffold_7G00201840
rosa_rugosa	Rorug02G0086200 Rorug06G0024500 Rorug06G0024900 Rorug06G0025000
rosa_samantha	Rh2AG135300 Rh2CG140500 Rh2DG140700 Rh6BG145900 Rh6CG142300 Rh6DG130400
rosa_wichuraiana	Rw2G010560 Rw6G012540 Rw6G012580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1026
AccB7I	CCANNNNNTGG	1 cut(s)	818
AccI	GTMKAC	1 cut(s)	926
AciI	CCGC	1 cut(s)	1488
AclI	AACGTT	1 cut(s)	1184
AclWI	GGATC	6 cut(s)	18, 500, 694, 875, 1973, 2294
AcoI	YGGCCR	2 cut(s)	73, 663
AcsI	RAATTY	5 cut(s)	202, 1400, 1989, 2093, 2153
AcuI	CTGAAG	2 cut(s)	155, 915
AfaI	GTAC	5 cut(s)	383, 390, 710, 1250, 2235
AfiI	CCNNNNNNNGG	4 cut(s)	89, 818, 1484, 2382
AflIII	ACRYGT	2 cut(s)	521, 705
AhdI	GACNNNNNGTC	1 cut(s)	752
AjnI	CCWGG	8 cut(s)	88, 342, 431, 590, 1195, 1548, 1587, 2128
AjuI	GAANNNNNNNTTGG	6 cut(s)	624, 656, 877, 909, 1621, 1653
AloI	GAACNNNNNNTCC	2 cut(s)	956, 988
Alw21I	GWGCWC	3 cut(s)	1344, 2018, 2350
Alw26I	GTCTC	3 cut(s)	44, 1121, 1136
Alw44I	GTGCAC	1 cut(s)	1340
AlwI	GGATC	6 cut(s)	18, 500, 694, 875, 1973, 2294
AlwNI	CAGNNNCTG	1 cut(s)	1373
AoxI	GGCC	8 cut(s)	73, 179, 349, 588, 663, 1522, 1768, 2131
ApaLI	GTGCAC	1 cut(s)	1340
ApeKI	GCWGC	9 cut(s)	410, 437, 808, 950, 983, 1453, 1654, 1670, 1684
ApoI	RAATTY	5 cut(s)	202, 1400, 1989, 2093, 2153
AseI	ATTAAT	1 cut(s)	1353
Asp700I	GAANNNNTTC	1 cut(s)	2094
AspLEI	GCGC	2 cut(s)	1453, 2325
AspS9I	GGNCC	8 cut(s)	179, 350, 469, 620, 746, 1523, 1581, 2181
AsuC2I	CCSGG	1 cut(s)	240
AsuHPI	GGTGA	6 cut(s)	136, 490, 620, 1003, 1838, 2266
AsuII	TTCGAA	1 cut(s)	962
AvaII	GGWCC	5 cut(s)	469, 620, 746, 1581, 2181
BaeGI	GKGCMC	1 cut(s)	1344
BalI	TGGCCA	2 cut(s)	75, 665
BanII	GRGCYC	1 cut(s)	159
BbsI	GAAGAC	1 cut(s)	143
Bbv12I	GWGCWC	3 cut(s)	1344, 2018, 2350
BbvI	GCAGC	9 cut(s)	397, 449, 820, 962, 970, 1440, 1641, 1657, 1696
BccI	CCATC	4 cut(s)	26, 439, 2052, 2147
BcgI	CGANNNNNNTGC	6 cut(s)	758, 792, 942, 976, 2158, 2192
BciT130I	CCWGG	8 cut(s)	90, 344, 433, 592, 1197, 1550, 1589, 2130
BciVI	GTATCC	2 cut(s)	1049, 1222
BcnI	CCSGG	1 cut(s)	240
BcoDI	GTCTC	3 cut(s)	44, 1121, 1136
BfaI	CTAG	4 cut(s)	1169, 1257, 2160, 2237
BfoI	RGCGCY	1 cut(s)	2326
BfuAI	ACCTGC	1 cut(s)	1026
BfuI	GTATCC	2 cut(s)	1049, 1222
BglII	AGATCT	2 cut(s)	1156, 2006
BisI	GCNGC	9 cut(s)	411, 438, 809, 951, 984, 1454, 1655, 1671, 1685
BlsI	GCNGC	9 cut(s)	412, 439, 810, 952, 985, 1455, 1656, 1672, 1686
Bme1390I	CCNGG	9 cut(s)	90, 240, 344, 433, 592, 1197, 1550, 1589, 2130
Bme18I	GGWCC	5 cut(s)	469, 620, 746, 1581, 2181
BmeRI	GACNNNNNGTC	1 cut(s)	752
BmgT120I	GGNCC	8 cut(s)	179, 350, 469, 620, 746, 1523, 1581, 2181
BmiI	GGNNCC	7 cut(s)	352, 470, 471, 721, 747, 1438, 2182
BmrFI	CCNGG	9 cut(s)	90, 240, 344, 433, 592, 1197, 1550, 1589, 2130
BmrI	ACTGGG	2 cut(s)	474, 1520
BmsI	GCATC	4 cut(s)	252, 487, 766, 1670
BmuI	ACTGGG	2 cut(s)	474, 1520
BpiI	GAAGAC	1 cut(s)	143
BplI	GAGNNNNNCTC	2 cut(s)	1287, 1319
BpmI	CTGGAG	4 cut(s)	486, 613, 1179, 1872
Bpu14I	TTCGAA	1 cut(s)	962
BpuMI	CCSGG	1 cut(s)	240
BsaAI	YACGTR	1 cut(s)	1430
BsaBI	GATNNNNATC	1 cut(s)	1155
BsaI	GGTCTC	1 cut(s)	44
Bsc4I	CCNNNNNNNGG	4 cut(s)	89, 818, 1484, 2382
Bse118I	RCCGGY	1 cut(s)	1412
Bse1I	ACTGG	7 cut(s)	469, 509, 638, 1526, 1811, 2066, 2134
Bse3DI	GCAATG	2 cut(s)	288, 1840
Bse8I	GATNNNNATC	1 cut(s)	1155
BseBI	CCWGG	8 cut(s)	90, 344, 433, 592, 1197, 1550, 1589, 2130
BseGI	GGATG	1 cut(s)	2044
BseJI	GATNNNNATC	1 cut(s)	1155
BseLI	CCNNNNNNNGG	4 cut(s)	89, 818, 1484, 2382
BseMI	GCAATG	2 cut(s)	288, 1840
BseMII	CTCAG	5 cut(s)	186, 288, 1471, 2028, 2343
BseNI	ACTGG	7 cut(s)	469, 509, 638, 1526, 1811, 2066, 2134
BseSI	GKGCMC	1 cut(s)	1344
BseXI	GCAGC	9 cut(s)	397, 449, 820, 962, 970, 1440, 1641, 1657, 1696
BsgI	GTGCAG	5 cut(s)	969, 1452, 1861, 2289, 2352
BshFI	GGCC	8 cut(s)	75, 181, 351, 590, 665, 1524, 1770, 2133
BsiHKAI	GWGCWC	3 cut(s)	1344, 2018, 2350
BsiSI	CCGG	2 cut(s)	239, 1413
BslFI	GGGAC	7 cut(s)	324, 325, 455, 759, 917, 1574, 2194
BslI	CCNNNNNNNGG	4 cut(s)	89, 818, 1484, 2382
BsmAI	GTCTC	3 cut(s)	44, 1121, 1136
BsmBI	CGTCTC	1 cut(s)	1121
BsmFI	GGGAC	7 cut(s)	324, 325, 455, 759, 917, 1574, 2194
BsnI	GGCC	8 cut(s)	75, 181, 351, 590, 665, 1524, 1770, 2133
Bso31I	GGTCTC	1 cut(s)	44
Bsp119I	TTCGAA	1 cut(s)	962
Bsp1286I	GDGCHC	4 cut(s)	159, 1344, 2018, 2350
Bsp143I	GATC	9 cut(s)	10, 118, 505, 686, 880, 1156, 1978, 2006, 2299
Bsp19I	CCATGG	3 cut(s)	473, 712, 1025
BspACI	CCGC	1 cut(s)	1488
BspANI	GGCC	8 cut(s)	75, 181, 351, 590, 665, 1524, 1770, 2133
BspCNI	CTCAG	5 cut(s)	187, 289, 1470, 2029, 2342
BspLI	GGNNCC	7 cut(s)	352, 470, 471, 721, 747, 1438, 2182
BspMI	ACCTGC	1 cut(s)	1026
BspPI	GGATC	6 cut(s)	18, 500, 694, 875, 1973, 2294
BspT104I	TTCGAA	1 cut(s)	962
BspTNI	GGTCTC	1 cut(s)	44
BsrDI	GCAATG	2 cut(s)	288, 1840
BsrFI	RCCGGY	1 cut(s)	1412
BsrI	ACTGG	7 cut(s)	469, 509, 638, 1526, 1811, 2066, 2134
BssAI	RCCGGY	1 cut(s)	1412
BssMI	GATC	9 cut(s)	10, 118, 505, 686, 880, 1156, 1978, 2006, 2299
BssNAI	GTATAC	1 cut(s)	927
BssT1I	CCWWGG	5 cut(s)	473, 712, 1025, 1519, 2106
Bst1107I	GTATAC	1 cut(s)	927
Bst2UI	CCWGG	8 cut(s)	90, 344, 433, 592, 1197, 1550, 1589, 2130
Bst4CI	ACNGT	3 cut(s)	539, 2020, 2288
Bst6I	CTCTTC	2 cut(s)	314, 2149
BstAPI	GCANNNNNTGC	3 cut(s)	1111, 1839, 2345
BstBAI	YACGTR	1 cut(s)	1430
BstBI	TTCGAA	1 cut(s)	962
BstC8I	GCNNGC	1 cut(s)	1659
BstDEI	CTNAG	5 cut(s)	195, 297, 1457, 2037, 2329
BstDSI	CCRYGG	3 cut(s)	473, 712, 1025
BstF5I	GGATG	1 cut(s)	2044
BstH2I	RGCGCY	1 cut(s)	2326
BstHHI	GCGC	2 cut(s)	1453, 2325
BstKTI	GATC	9 cut(s)	13, 121, 508, 689, 883, 1159, 1981, 2009, 2302
BstMAI	GTCTC	3 cut(s)	44, 1121, 1136
BstMBI	GATC	9 cut(s)	10, 118, 505, 686, 880, 1156, 1978, 2006, 2299
BstMWI	GCNNNNNNNGC	6 cut(s)	446, 1111, 1667, 1839, 2033, 2345
BstNI	CCWGG	8 cut(s)	90, 344, 433, 592, 1197, 1550, 1589, 2130
BstNSI	RCATGY	3 cut(s)	525, 709, 1009
BstSCI	CCNGG	9 cut(s)	88, 238, 342, 431, 590, 1195, 1548, 1587, 2128
BstSLI	GKGCMC	1 cut(s)	1344
BstV1I	GCAGC	9 cut(s)	397, 449, 820, 962, 970, 1440, 1641, 1657, 1696
BstV2I	GAAGAC	1 cut(s)	143
BstX2I	RGATCY	5 cut(s)	10, 505, 1156, 2006, 2299
BstXI	CCANNNNNNTGG	1 cut(s)	1695
BstYI	RGATCY	5 cut(s)	10, 505, 1156, 2006, 2299
BstZ17I	GTATAC	1 cut(s)	927
BsuI	GTATCC	2 cut(s)	1049, 1222
BsuRI	GGCC	8 cut(s)	75, 181, 351, 590, 665, 1524, 1770, 2133
BtgI	CCRYGG	3 cut(s)	473, 712, 1025
BtsCI	GGATG	1 cut(s)	2044
BtsI	GCAGTG	3 cut(s)	666, 1071, 1188
BtsIMutI	CAGTG	6 cut(s)	516, 666, 1071, 1188, 1465, 2016
BveI	ACCTGC	1 cut(s)	1026
Cac8I	GCNNGC	1 cut(s)	1659
CaiI	CAGNNNCTG	1 cut(s)	1373
CfoI	GCGC	2 cut(s)	1453, 2325
Cfr10I	RCCGGY	1 cut(s)	1412
Cfr13I	GGNCC	8 cut(s)	179, 350, 469, 620, 746, 1523, 1581, 2181
CseI	GACGC	2 cut(s)	741, 1138
CsiI	ACCWGGT	1 cut(s)	1587
Csp6I	GTAC	5 cut(s)	382, 389, 709, 1249, 2234
CviQI	GTAC	5 cut(s)	382, 389, 709, 1249, 2234
DdeI	CTNAG	5 cut(s)	195, 297, 1457, 2037, 2329
DpnI	GATC	9 cut(s)	12, 120, 507, 688, 882, 1158, 1980, 2008, 2301
DpnII	GATC	9 cut(s)	10, 118, 505, 686, 880, 1156, 1978, 2006, 2299
DriI	GACNNNNNGTC	1 cut(s)	752
EaeI	YGGCCR	2 cut(s)	73, 663
Eam1104I	CTCTTC	2 cut(s)	314, 2149
Eam1105I	GACNNNNNGTC	1 cut(s)	752
EarI	CTCTTC	2 cut(s)	314, 2149
EciI	GGCGGA	1 cut(s)	1503
Eco130I	CCWWGG	5 cut(s)	473, 712, 1025, 1519, 2106
Eco147I	AGGCCT	1 cut(s)	590
Eco24I	GRGCYC	1 cut(s)	159
Eco31I	GGTCTC	1 cut(s)	44
Eco47I	GGWCC	5 cut(s)	469, 620, 746, 1581, 2181
Eco57I	CTGAAG	2 cut(s)	155, 915
EcoO109I	RGGNCCY	4 cut(s)	350, 469, 746, 2181
EcoRII	CCWGG	8 cut(s)	88, 342, 431, 590, 1195, 1548, 1587, 2128
EcoT14I	CCWWGG	5 cut(s)	473, 712, 1025, 1519, 2106
EcoT38I	GRGCYC	1 cut(s)	159
ErhI	CCWWGG	5 cut(s)	473, 712, 1025, 1519, 2106
Esp3I	CGTCTC	1 cut(s)	1121
FalI	AAGNNNNNCTT	2 cut(s)	2099, 2131
FaqI	GGGAC	7 cut(s)	324, 325, 455, 759, 917, 1574, 2194
FauNDI	CATATG	2 cut(s)	655, 1147
FblI	GTMKAC	1 cut(s)	926
Fnu4HI	GCNGC	9 cut(s)	411, 438, 809, 951, 984, 1454, 1655, 1671, 1685
FokI	GGATG	1 cut(s)	2031
FriOI	GRGCYC	1 cut(s)	159
Fsp4HI	GCNGC	9 cut(s)	411, 438, 809, 951, 984, 1454, 1655, 1671, 1685
FspBI	CTAG	4 cut(s)	1169, 1257, 2160, 2237
GlaI	GCGC	2 cut(s)	1452, 2324
GluI	GCNGC	9 cut(s)	411, 438, 809, 951, 984, 1454, 1655, 1671, 1685
GsuI	CTGGAG	4 cut(s)	486, 613, 1179, 1872
HaeII	RGCGCY	1 cut(s)	2326
HaeIII	GGCC	8 cut(s)	75, 181, 351, 590, 665, 1524, 1770, 2133
HapII	CCGG	2 cut(s)	239, 1413
HgaI	GACGC	2 cut(s)	741, 1138
HhaI	GCGC	2 cut(s)	1453, 2325
Hin6I	GCGC	2 cut(s)	1451, 2323
HinP1I	GCGC	2 cut(s)	1451, 2323
HincII	GTYRAC	3 cut(s)	1264, 1326, 1892
HindII	GTYRAC	3 cut(s)	1264, 1326, 1892
HinfI	GANTC	7 cut(s)	129, 338, 542, 669, 935, 1280, 1515
HpaII	CCGG	2 cut(s)	239, 1413
HphI	GGTGA	6 cut(s)	136, 490, 620, 1003, 1838, 2266
Hpy188I	TCNGA	5 cut(s)	15, 934, 1279, 1862, 2310
HpyAV	CCTTC	7 cut(s)	16, 101, 823, 1052, 1294, 1992, 2305
HpyCH4III	ACNGT	3 cut(s)	539, 2020, 2288
HpyCH4IV	ACGT	3 cut(s)	1184, 1429, 2031
HpyF10VI	GCNNNNNNNGC	6 cut(s)	446, 1111, 1667, 1839, 2033, 2345
HpyF3I	CTNAG	5 cut(s)	195, 297, 1457, 2037, 2329
HpySE526I	ACGT	3 cut(s)	1184, 1429, 2031
HspAI	GCGC	2 cut(s)	1451, 2323
KflI	GGGWCCC	1 cut(s)	469
Kzo9I	GATC	9 cut(s)	10, 118, 505, 686, 880, 1156, 1978, 2006, 2299
LmnI	GCTCC	6 cut(s)	407, 505, 784, 832, 1198, 2353
Lsp1109I	GCAGC	9 cut(s)	397, 449, 820, 962, 970, 1440, 1641, 1657, 1696
LweI	GCATC	4 cut(s)	252, 487, 766, 1670
MabI	ACCWGGT	1 cut(s)	1587
MaeI	CTAG	4 cut(s)	1169, 1257, 2160, 2237
MaeII	ACGT	3 cut(s)	1184, 1429, 2031
MaeIII	GTNAC	5 cut(s)	754, 1219, 2123, 2272, 2288
MalI	GATC	9 cut(s)	12, 120, 507, 688, 882, 1158, 1980, 2008, 2301
MboI	GATC	9 cut(s)	10, 118, 505, 686, 880, 1156, 1978, 2006, 2299
MfeI	CAATTG	1 cut(s)	2377
MflI	RGATCY	5 cut(s)	10, 505, 1156, 2006, 2299
MhlI	GDGCHC	4 cut(s)	159, 1344, 2018, 2350
MlsI	TGGCCA	2 cut(s)	75, 665
MluNI	TGGCCA	2 cut(s)	75, 665
MlyI	GAGTC	1 cut(s)	347
MmeI	TCCRAC	6 cut(s)	1063, 1185, 1450, 1573, 2072, 2307
Mox20I	TGGCCA	2 cut(s)	75, 665
MroXI	GAANNNNTTC	1 cut(s)	2094
MscI	TGGCCA	2 cut(s)	75, 665
MseI	TTAA	9 cut(s)	696, 800, 849, 999, 1068, 1353, 1782, 1931, 2151
Msp20I	TGGCCA	2 cut(s)	75, 665
MspA1I	CMGCKG	2 cut(s)	878, 983
MspI	CCGG	2 cut(s)	239, 1413
MspR9I	CCNGG	9 cut(s)	90, 240, 344, 433, 592, 1197, 1550, 1589, 2130
MunI	CAATTG	1 cut(s)	2377
MvaI	CCWGG	8 cut(s)	90, 344, 433, 592, 1197, 1550, 1589, 2130
MwoI	GCNNNNNNNGC	6 cut(s)	446, 1111, 1667, 1839, 2033, 2345
NciI	CCSGG	1 cut(s)	240
NcoI	CCATGG	3 cut(s)	473, 712, 1025
NdeI	CATATG	2 cut(s)	655, 1147
NdeII	GATC	9 cut(s)	10, 118, 505, 686, 880, 1156, 1978, 2006, 2299
NlaIV	GGNNCC	7 cut(s)	352, 470, 471, 721, 747, 1438, 2182
NmuCI	GTSAC	2 cut(s)	754, 2272
NspI	RCATGY	3 cut(s)	525, 709, 1009
NspV	TTCGAA	1 cut(s)	962
PasI	CCCWGGG	1 cut(s)	343
PceI	AGGCCT	1 cut(s)	590
PciI	ACATGT	2 cut(s)	521, 705
PdmI	GAANNNNTTC	1 cut(s)	2094
PfeI	GAWTC	6 cut(s)	129, 542, 669, 935, 1280, 1515
PflMI	CCANNNNNTGG	1 cut(s)	818
PkrI	GCNGC	9 cut(s)	412, 439, 810, 952, 985, 1455, 1656, 1672, 1686
PleI	GAGTC	1 cut(s)	346
PpsI	GAGTC	1 cut(s)	346
Ppu21I	YACGTR	1 cut(s)	1430
PpuMI	RGGWCCY	3 cut(s)	469, 746, 2181
PscI	ACATGT	2 cut(s)	521, 705
PshBI	ATTAAT	1 cut(s)	1353
Psp1406I	AACGTT	1 cut(s)	1184
Psp5II	RGGWCCY	3 cut(s)	469, 746, 2181
Psp6I	CCWGG	8 cut(s)	88, 342, 431, 590, 1195, 1548, 1587, 2128
PspGI	CCWGG	8 cut(s)	88, 342, 431, 590, 1195, 1548, 1587, 2128
PspN4I	GGNNCC	7 cut(s)	352, 470, 471, 721, 747, 1438, 2182
PspPI	GGNCC	8 cut(s)	179, 350, 469, 620, 746, 1523, 1581, 2181
PspPPI	RGGWCCY	3 cut(s)	469, 746, 2181
PstNI	CAGNNNCTG	1 cut(s)	1373
PsuI	RGATCY	5 cut(s)	10, 505, 1156, 2006, 2299
PvuII	CAGCTG	2 cut(s)	878, 983
RsaI	GTAC	5 cut(s)	383, 390, 710, 1250, 2235
RsaNI	GTAC	5 cut(s)	382, 389, 709, 1249, 2234
SaqAI	TTAA	9 cut(s)	696, 800, 849, 999, 1068, 1353, 1782, 1931, 2151
SatI	GCNGC	9 cut(s)	411, 438, 809, 951, 984, 1454, 1655, 1671, 1685
Sau3AI	GATC	9 cut(s)	10, 118, 505, 686, 880, 1156, 1978, 2006, 2299
Sau96I	GGNCC	8 cut(s)	179, 350, 469, 620, 746, 1523, 1581, 2181
SchI	GAGTC	1 cut(s)	347
ScrFI	CCNGG	9 cut(s)	90, 240, 344, 433, 592, 1197, 1550, 1589, 2130
SduI	GDGCHC	4 cut(s)	159, 1344, 2018, 2350
SexAI	ACCWGGT	1 cut(s)	1587
SfaNI	GCATC	4 cut(s)	252, 487, 766, 1670
SfuI	TTCGAA	1 cut(s)	962
SinI	GGWCC	5 cut(s)	469, 620, 746, 1581, 2181
SseBI	AGGCCT	1 cut(s)	590
SsiI	CCGC	1 cut(s)	1488
SspMI	CTAG	4 cut(s)	1169, 1257, 2160, 2237
StuI	AGGCCT	1 cut(s)	590
StyD4I	CCNGG	9 cut(s)	88, 238, 342, 431, 590, 1195, 1548, 1587, 2128
StyI	CCWWGG	5 cut(s)	473, 712, 1025, 1519, 2106
TaaI	ACNGT	3 cut(s)	539, 2020, 2288
TaiI	ACGT	3 cut(s)	1187, 1432, 2034
TaqI	TCGA	4 cut(s)	42, 336, 962, 2219
TaqII	GACCGA	1 cut(s)	1911
TatI	WGTACW	2 cut(s)	388, 2233
TfiI	GAWTC	6 cut(s)	129, 542, 669, 935, 1280, 1515
Tru1I	TTAA	9 cut(s)	696, 800, 849, 999, 1068, 1353, 1782, 1931, 2151
Tru9I	TTAA	9 cut(s)	696, 800, 849, 999, 1068, 1353, 1782, 1931, 2151
TscAI	CASTG	6 cut(s)	516, 666, 1078, 1195, 1465, 2023
TseFI	GTSAC	2 cut(s)	754, 2272
TseI	GCWGC	9 cut(s)	410, 437, 808, 950, 983, 1453, 1654, 1670, 1684
Tsp45I	GTSAC	2 cut(s)	754, 2272
TspDTI	ATGAA	9 cut(s)	93, 332, 661, 804, 827, 1167, 1263, 1339, 1526
TspGWI	ACGGA	4 cut(s)	399, 623, 696, 1740
TspRI	CASTG	6 cut(s)	516, 666, 1078, 1195, 1465, 2023
Van91I	CCANNNNNTGG	1 cut(s)	818
VneI	GTGCAC	1 cut(s)	1340
VpaK11BI	GGWCC	5 cut(s)	469, 620, 746, 1581, 2181
VspI	ATTAAT	1 cut(s)	1353
XapI	RAATTY	5 cut(s)	202, 1400, 1989, 2093, 2153
XbaI	TCTAGA	1 cut(s)	1168
XceI	RCATGY	3 cut(s)	525, 709, 1009
XmiI	GTMKAC	1 cut(s)	926
XmnI	GAANNNNTTC	1 cut(s)	2094
XspI	CTAG	4 cut(s)	1169, 1257, 2160, 2237

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.