Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000174

Orthogroup ID

OG0001965

Physical Location & Seq

Genomic Coordinates Forward (+)

413880 .. 420263

Gene Structure 6384 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_174G00435340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGGAGCTAGCTCAGCTTTGAGCTGGGTTCTGCTCTGCTTAGTTGTGTTGTCTCTACGTTTTCAGCTGATACTGTGCAATGTTAGCTATGACAGGAAGGCTCTTATCATTAATGGGCAGAGGAGAATTCTCTTTTCTGGGTCCATACACTATCCTAGAAGTACTCCTGAGATGTGGGAAGGTCTTATAGAGAAGGCCAAAGATGGTGGGTTGGATGTTATAGACACCTATGTGTTTTGGAATCTCCACGAGCCTTCTCCTGGCAATTACAATTTTGAAGGGAGAAATGATTTGGTTCGCTTCATTAAGTTAGTCCAAGAGGCACGGCTCTATGTGCATCTTCGCATTGGGCCGTACATTTGCTCAGAATGGAATTTTGGGGGATTTCCAGTTTGGCTCAAGTATGTTCCAGGCATTAGCTTCAGAACAGATAATGAGCCTTTCAAGTCAGCAATGAAAAAATTTACCAAGAAGATTGTTCAATTGATGATGGATGAAAAGCTATTTGAGTCCCAAGGTGGCCCCATCATCCTTTCTCAGATTGAGAATGAATATGAGCCAGAAGGCAAGCTTTTTGGTGCTGCTGGTCATGCATACATGACTTGGGCTGCAGAGATGGCTGTTGGAATGGGTACTGGTGTTCCATGGGTCATGTGCAAGGAAGAGGATGCCCCAGACCCAGTGATAAACACTTGCAATGGTTTTTATTGCGACTACTTTTCTCCAAACAAACCATATAAACCCACACTTTGGACTGAGGCTTGGACTGGCTGGTTTACAGAATTTGGTGGCCCAATTTACAAGCGACCTGTTGAAGATTTGGCGTTTGGAGTTGCCCGATTCATTCAGAATGGAGGATCCTTCATAAATTATTACATGTACCATGGAGGAACAAACTTTGGAAGAACTGCAGGAGGTCCATTTATTACTACCAGTTATGACTATGATGCTCCCATTGACGAATATGGCTTGATCAGGCAACCTAAATATGGCCATTTAAAGGAACTCCATAAGGCTGTTAAGTTATGTGAGCAAGCTCTGCTAAACACTGATTCTACTGTTACATCCTTAGGGACTTATGAACAGGCACACGTATTCTCTTCCAAGTCAGGAGATTGTGCAGCTTTTCTCTCAAATTACAATACAAAGTCAGCTGCAACTGTGACTTTCAATCAGATGAACTATCACCTCCCACCTTGGTCCATCAGCATCCTGCCAGATTGTAAAAACGTTGTATACAACACTGCCCAAGTGGGAGTTCAAACATCCCAAAAACAGTTTTTTCCTACTAACTCTAAGTTGCCCTCATGGGAAACCTTCAGTGAAGACATCTCTTCAGTTGCTAGCAATACGAAACTCACAGTAATTGGTCTCTTAGATCAGTTGAACATCACCAGAGATTCCAGTGATTACTTATGGTACACAACCAATATCGACATTAAATCATCTGAATCATTTCTGCGTGGCGGTCAACATCCAACTCTGACCGTGCAATCAGCTGGTGATGCTATGCATGTCTTTATCAATGGGCAGCTCTCAGGATCGGCGTATGGGACTAGGAAAAACAAGAGATTCACTTTTACAGGAAATGTCATCCTGCATGCTGGATTGAATAAAATCTCACTTCTCAGTATAGCTGTTGGATTGCCGAACAATGGTCCTCACTTTGAGACACGGAATGCTGGAGTACTGGGACCAGTTGCTCTACATGGACTAGACCAAGGAAGCAGAGACTTATCATGGCAGAAATGGTCGCACGAGGTGGGCCTAAAAGGAGAAGCCATGAACCTGGGTTCTCCAAGTTCCATCTCGACAGTGGATTGGACGAGGGCGTCCTTAGCGGTGAAGAATCAACAGCCACTGACGTGGTATAAGGTCAATTTTGATGCACCTGAAGGAGATGAACCTTTGGCTTTGGACATGGGAAGTATGGGCAAGGGTCAACTATGGATCAATGGGCAAAGCATCGGAAGATACTGGACTACTTATGCTAACGGGGATTGCAGTGCGTGCAGTTATTCTGGTACCTTCAGGCCTAAGAATAAGTGCCAATATGGTTGTAACCATCCAACTCAACAATGGTACCATGTTCCTAGGTCCTGGTTAAAGCAATCTAAAAACCTATTGGTAGTTTTTGAGGAGATTGGTGGGGATGTATCAAAGATTGCTCTTGTCAAACGTTCAGTGAGCACTGTTTGTGCTGAGGTTTCTGAAAATCACCCACATAATAGGAATTGGCATACTGAGAGCCGTGGCCAACTGGAAGAGCAAAAGAAGCCTATAATGAGCCTACACTGTACGGATGGATACTCCATTTCTGCTATTAAGTTTTCAAGTTTTGGAACTCCATCTGGAAGCTGTGGGAATTTTCAGCACGGTACATGTCACGCTCCAAACTCGAAGGCTGTCATTGAGAAGAAGTGTATAGGTAAGCAGCAATGTTCATTGACCATATCAGATGCCAATTTTGGGATGGATCCATGTCCAAGCATGCTGAAAAAATTATCTGTTGAAGCTGTTTGTGCCCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0009505 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

843

Amino Acids

93.73

Weight (kDa)

7.74

Isoelectric Point (pI)

39.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 339	4.2e-122	Glycosyl hydrolases family 35
GHD	PF17834	362 - 418	4.2e-25	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	471 - 538	1.8e-08	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	621 - 707	5.3e-27	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	764 - 841	7.5e-21	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36360 AT4G36360
fragaria_vesca	FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_2g09820 FvH4_2g09820
malus_domestica	MD02G1137500.v1.1 MD10G1067800.v1.1 MD15G1251100.v1.1
prunus_persica	Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.8G089500_v2.0.a1 Prupe.8G089500_v2.0.a1
pyrus_communis	pycom02g10880 pycom10g05360 pycom15g22140
rosa_chinensis	RchiOBHm_Chr2g0100171 RchiOBHm_Chr6g0266171
rosa_laevigata	RLG00000014106 RLG00000014109 RLG00000016943
rosa_multiflora	Rmu_sc0002547.1_g000001
rosa_roxburghii	Rroxscaffold_174G00435340 Rroxscaffold_2G00142500 Rroxscaffold_7G00201780 Rroxscaffold_7G00201840
rosa_rugosa	Rorug02G0086200 Rorug06G0024500 Rorug06G0024900 Rorug06G0025000
rosa_samantha	Rh2AG135300 Rh2CG140500 Rh2DG140700 Rh6BG145900 Rh6CG142300 Rh6DG130400
rosa_wichuraiana	Rw2G010560 Rw6G012540 Rw6G012580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1831
Acc65I	GGTACC	2 cut(s)	2024, 2082
AccB1I	GGYRCC	2 cut(s)	2024, 2082
AccB7I	CCANNNNNTGG	1 cut(s)	750
AccI	GTMKAC	1 cut(s)	1236
AciI	CCGC	2 cut(s)	1467, 1841
AclI	AACGTT	2 cut(s)	1230, 2179
AclWI	GGATC	6 cut(s)	852, 865, 1549, 1958, 2471, 2484
AcoI	YGGCCR	2 cut(s)	991, 2254
AcsI	RAATTY	5 cut(s)	126, 373, 461, 782, 2365
AcuI	CTGAAG	5 cut(s)	406, 1303, 1320, 1914, 2014
AcyI	GRCGYC	1 cut(s)	1832
AdeI	CACNNNGTG	1 cut(s)	1762
AfiI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1655, 2469
AflIII	ACRYGT	3 cut(s)	876, 1090, 2381
AjiI	CACGTC	1 cut(s)	1866
AjnI	CCWGG	4 cut(s)	259, 409, 1788, 2099
AjuI	GAANNNNNNNTTGG	4 cut(s)	360, 392, 1242, 1274
AleI	CACNNNNGTG	2 cut(s)	230, 1864
Alw21I	GWGCWC	1 cut(s)	2192
Alw26I	GTCTC	4 cut(s)	57, 1376, 1664, 1725
AlwI	GGATC	6 cut(s)	852, 865, 1549, 1958, 2471, 2484
AlwNI	CAGNNNCTG	1 cut(s)	1861
AoxI	GGCC	8 cut(s)	195, 350, 520, 790, 991, 1765, 2033, 2254
ApeKI	GCWGC	6 cut(s)	581, 608, 1121, 1154, 1531, 2434
ApoI	RAATTY	5 cut(s)	126, 373, 461, 782, 2365
AseI	ATTAAT	1 cut(s)	111
Asp700I	GAANNNNTTC	1 cut(s)	1455
Asp718I	GGTACC	2 cut(s)	2024, 2082
AspA2I	CCTAGG	1 cut(s)	2093
AsuHPI	GGTGA	5 cut(s)	1178, 1384, 1514, 1855, 2210
AsuNHI	GCTAGC	2 cut(s)	8, 1343
AvaII	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
AvrII	CCTAGG	1 cut(s)	2093
AxyI	CCTNAGG	1 cut(s)	1069
BaeGI	GKGCMC	1 cut(s)	2527
BaeI	ACNNNNGTAYC	4 cut(s)	624, 657, 2066, 2099
BalI	TGGCCA	2 cut(s)	993, 2256
BamHI	GGATCC	2 cut(s)	857, 2476
BanI	GGYRCC	2 cut(s)	2024, 2082
BauI	CACGAG	2 cut(s)	248, 1757
BbsI	GAAGAC	1 cut(s)	1332
Bbv12I	GWGCWC	1 cut(s)	2192
BbvCI	CCTCAGC	1 cut(s)	2202
BbvI	GCAGC	6 cut(s)	568, 595, 1133, 1141, 1543, 2446
BceAI	ACGGC	3 cut(s)	337, 341, 2235
BciT130I	CCWGG	4 cut(s)	261, 411, 1790, 2101
BciVI	GTATCC	1 cut(s)	2300
BclI	TGATCA	1 cut(s)	972
BcoDI	GTCTC	4 cut(s)	57, 1376, 1664, 1725
BfaI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
BfmI	CTRYAG	2 cut(s)	609, 909
BfuI	GTATCC	1 cut(s)	2300
BisI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 2435
BlnI	CCTAGG	1 cut(s)	2093
BlpI	GCTNAGC	1 cut(s)	13
BlsI	GCNGC	6 cut(s)	583, 610, 1123, 1156, 1533, 2436
BmcAI	AGTACT	2 cut(s)	163, 1689
Bme1390I	CCNGG	4 cut(s)	261, 411, 1790, 2101
Bme18I	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
BmgBI	CACGTC	1 cut(s)	1866
BmiI	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
BmrFI	CCNGG	4 cut(s)	261, 411, 1790, 2101
BmrI	ACTGGG	2 cut(s)	674, 1700
BmsI	GCATC	8 cut(s)	346, 658, 937, 1218, 1495, 1876, 1974, 2449
BmtI	GCTAGC	2 cut(s)	12, 1347
BmuI	ACTGGG	2 cut(s)	674, 1700
BpiI	GAAGAC	1 cut(s)	1332
BpmI	CTGGAG	1 cut(s)	1704
Bpu10I	CCTNAGC	2 cut(s)	1837, 2202
Bpu1102I	GCTNAGC	1 cut(s)	13
BpuEI	CTTGAG	1 cut(s)	383
BsaAI	YACGTR	1 cut(s)	1093
BsaHI	GRCGYC	1 cut(s)	1832
BsaI	GGTCTC	1 cut(s)	1376
BsaJI	CCNNGG	8 cut(s)	514, 644, 883, 1196, 1720, 1789, 2093, 2251
Bsc4I	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1655, 2469
Bse21I	CCTNAGG	1 cut(s)	1069
Bse3DI	GCAATG	4 cut(s)	85, 459, 703, 2444
BseBI	CCWGG	4 cut(s)	261, 411, 1790, 2101
BseDI	CCNNGG	8 cut(s)	514, 644, 883, 1196, 1720, 1789, 2093, 2251
BseLI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1655, 2469
BseMI	GCAATG	4 cut(s)	85, 459, 703, 2444
BseMII	CTCAG	9 cut(s)	27, 159, 378, 551, 747, 1551, 1642, 2193, 2235
BseRI	GAGGAG	2 cut(s)	136, 2153
BseSI	GKGCMC	1 cut(s)	2527
BseXI	GCAGC	6 cut(s)	568, 595, 1133, 1141, 1543, 2446
BseYI	CCCAGC	1 cut(s)	24
BsgI	GTGCAG	2 cut(s)	1140, 2032
BshFI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
BshNI	GGYRCC	2 cut(s)	2024, 2082
BsiHKAI	GWGCWC	1 cut(s)	2192
BslFI	GGGAC	4 cut(s)	496, 1087, 1567, 1707
BslI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1655, 2469
BsmAI	GTCTC	4 cut(s)	57, 1376, 1664, 1725
BsmFI	GGGAC	4 cut(s)	496, 1087, 1567, 1707
BsmI	GAATGC	1 cut(s)	1684
BsnI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
Bso31I	GGTCTC	1 cut(s)	1376
Bsp1286I	GDGCHC	2 cut(s)	2192, 2527
Bsp143I	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
Bsp1720I	GCTNAGC	1 cut(s)	13
Bsp19I	CCATGG	2 cut(s)	644, 883
BspACI	CCGC	2 cut(s)	1467, 1841
BspANI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
BspCNI	CTCAG	9 cut(s)	26, 160, 377, 550, 748, 1550, 1641, 2194, 2236
BspLI	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
BspMAI	CTGCAG	2 cut(s)	613, 913
BspOI	GCTAGC	2 cut(s)	12, 1347
BspPI	GGATC	6 cut(s)	852, 865, 1549, 1958, 2471, 2484
BspQI	GCTCTTC	1 cut(s)	2259
BspT107I	GGYRCC	2 cut(s)	2024, 2082
BspTNI	GGTCTC	1 cut(s)	1376
BsrDI	GCAATG	4 cut(s)	85, 459, 703, 2444
BssECI	CCNNGG	8 cut(s)	514, 644, 883, 1196, 1720, 1789, 2093, 2251
BssMI	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
BssNAI	GTATAC	1 cut(s)	1237
BssNI	GRCGYC	1 cut(s)	1832
BssSI	CACGAG	2 cut(s)	248, 1757
BssT1I	CCWWGG	6 cut(s)	514, 644, 883, 1196, 1720, 2093
Bst1107I	GTATAC	1 cut(s)	1237
Bst2BI	CACGAG	2 cut(s)	248, 1757
Bst2UI	CCWGG	4 cut(s)	261, 411, 1790, 2101
Bst6I	CTCTTC	4 cut(s)	657, 1105, 1339, 2259
BstACI	GRCGYC	1 cut(s)	1832
BstAPI	GCANNNNNTGC	2 cut(s)	1039, 2010
BstBAI	YACGTR	1 cut(s)	1093
BstC8I	GCNNGC	7 cut(s)	10, 569, 1035, 1345, 1602, 2011, 2492
BstDSI	CCRYGG	3 cut(s)	644, 883, 2251
BstKTI	GATC	6 cut(s)	860, 975, 1381, 1544, 1953, 2479
BstMAI	GTCTC	4 cut(s)	57, 1376, 1664, 1725
BstMBI	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
BstNI	CCWGG	4 cut(s)	261, 411, 1790, 2101
BstNSI	RCATGY	5 cut(s)	880, 1517, 1604, 2385, 2494
BstSCI	CCNGG	4 cut(s)	259, 409, 1788, 2099
BstSFI	CTRYAG	2 cut(s)	609, 909
BstSLI	GKGCMC	1 cut(s)	2527
BstV1I	GCAGC	6 cut(s)	568, 595, 1133, 1141, 1543, 2446
BstV2I	GAAGAC	1 cut(s)	1332
BstX2I	RGATCY	2 cut(s)	857, 2476
BstXI	CCANNNNNNTGG	2 cut(s)	1789, 1866
BstYI	RGATCY	2 cut(s)	857, 2476
BstZ17I	GTATAC	1 cut(s)	1237
Bsu36I	CCTNAGG	1 cut(s)	1069
BsuI	GTATCC	1 cut(s)	2300
BsuRI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
BtgI	CCRYGG	3 cut(s)	644, 883, 2251
BtrI	CACGTC	1 cut(s)	1866
BtsI	GCAGTG	2 cut(s)	1242, 2011
Cac8I	GCNNGC	7 cut(s)	10, 569, 1035, 1345, 1602, 2011, 2492
CaiI	CAGNNNCTG	1 cut(s)	1861
CseI	GACGC	1 cut(s)	1821
CspCI	CAANNNNNGTGG	2 cut(s)	187, 222
DpnI	GATC	6 cut(s)	859, 974, 1380, 1543, 1952, 2478
DpnII	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
DraI	TTTAAA	1 cut(s)	999
DraIII	CACNNNGTG	1 cut(s)	1762
DrdI	GACNNNNNNGTC	1 cut(s)	1831
DseDI	GACNNNNNNGTC	1 cut(s)	1831
EaeI	YGGCCR	2 cut(s)	991, 2254
Eam1104I	CTCTTC	4 cut(s)	657, 1105, 1339, 2259
EarI	CTCTTC	4 cut(s)	657, 1105, 1339, 2259
Eco130I	CCWWGG	6 cut(s)	514, 644, 883, 1196, 1720, 2093
Eco147I	AGGCCT	1 cut(s)	2035
Eco31I	GGTCTC	1 cut(s)	1376
Eco47I	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
Eco57I	CTGAAG	5 cut(s)	406, 1303, 1320, 1914, 2014
Eco81I	CCTNAGG	1 cut(s)	1069
EcoO109I	RGGNCCY	1 cut(s)	2097
EcoRI	GAATTC	1 cut(s)	126
EcoRII	CCWGG	4 cut(s)	259, 409, 1788, 2099
EcoT14I	CCWWGG	6 cut(s)	514, 644, 883, 1196, 1720, 2093
EcoT22I	ATGCAT	2 cut(s)	595, 1515
ErhI	CCWWGG	6 cut(s)	514, 644, 883, 1196, 1720, 2093
FalI	AAGNNNNNCTT	2 cut(s)	555, 587
FaqI	GGGAC	4 cut(s)	496, 1087, 1567, 1707
FbaI	TGATCA	1 cut(s)	972
FblI	GTMKAC	1 cut(s)	1236
Fnu4HI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 2435
Fsp4HI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 2435
FspBI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
GluI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 2435
GsaI	CCCAGC	1 cut(s)	28
GsuI	CTGGAG	1 cut(s)	1704
HaeIII	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
HgaI	GACGC	1 cut(s)	1821
Hin1I	GRCGYC	1 cut(s)	1832
HincII	GTYRAC	2 cut(s)	1472, 1943
HindII	GTYRAC	2 cut(s)	1472, 1943
HindIII	AAGCTT	1 cut(s)	569
HinfI	GANTC	8 cut(s)	241, 509, 840, 1052, 1400, 1451, 1572, 1849
HphI	GGTGA	5 cut(s)	1178, 1384, 1514, 1855, 2210
Hpy166II	GTNNAC	5 cut(s)	777, 1237, 1422, 1472, 1943
Hpy188III	TCNNGA	5 cut(s)	167, 1110, 1539, 1810, 2352
Hpy8I	GTNNAC	5 cut(s)	777, 1237, 1422, 1472, 1943
HpyCH4IV	ACGT	5 cut(s)	58, 1092, 1230, 1865, 2179
HpySE526I	ACGT	5 cut(s)	58, 1092, 1230, 1865, 2179
Hsp92I	GRCGYC	1 cut(s)	1832
KpnI	GGTACC	2 cut(s)	2028, 2086
Ksp22I	TGATCA	1 cut(s)	972
Kzo9I	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
LguI	GCTCTTC	1 cut(s)	2259
LmnI	GCTCC	3 cut(s)	5, 955, 2395
Lsp1109I	GCAGC	6 cut(s)	568, 595, 1133, 1141, 1543, 2446
LweI	GCATC	8 cut(s)	346, 658, 937, 1218, 1495, 1876, 1974, 2449
MaeI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
MaeII	ACGT	5 cut(s)	58, 1092, 1230, 1865, 2179
MaeIII	GTNAC	4 cut(s)	1060, 1162, 2060, 2384
MalI	GATC	6 cut(s)	859, 974, 1380, 1543, 1952, 2478
MboI	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
MfeI	CAATTG	1 cut(s)	482
MflI	RGATCY	2 cut(s)	857, 2476
MhlI	GDGCHC	2 cut(s)	2192, 2527
MlsI	TGGCCA	2 cut(s)	993, 2256
MluNI	TGGCCA	2 cut(s)	993, 2256
MlyI	GAGTC	1 cut(s)	518
MmeI	TCCRAC	5 cut(s)	192, 604, 1502, 1621, 2093
Mox20I	TGGCCA	2 cut(s)	993, 2256
Mph1103I	ATGCAT	2 cut(s)	595, 1515
MroXI	GAANNNNTTC	1 cut(s)	1455
MscI	TGGCCA	2 cut(s)	993, 2256
MseI	TTAA	7 cut(s)	111, 306, 998, 1020, 1440, 2105, 2325
MslI	CAYNNNNRTG	2 cut(s)	230, 1864
Msp20I	TGGCCA	2 cut(s)	993, 2256
MspA1I	CMGCKG	3 cut(s)	67, 1154, 1499
MspR9I	CCNGG	4 cut(s)	261, 411, 1790, 2101
MunI	CAATTG	1 cut(s)	482
Mva1269I	GAATGC	1 cut(s)	1684
MvaI	CCWGG	4 cut(s)	261, 411, 1790, 2101
NcoI	CCATGG	2 cut(s)	644, 883
NdeII	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
NheI	GCTAGC	2 cut(s)	8, 1343
NlaIV	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
NmuCI	GTSAC	2 cut(s)	1162, 2384
NsiI	ATGCAT	2 cut(s)	595, 1515
NspI	RCATGY	5 cut(s)	880, 1517, 1604, 2385, 2494
OliI	CACNNNNGTG	2 cut(s)	230, 1864
PaeI	GCATGC	2 cut(s)	1604, 2494
PceI	AGGCCT	1 cut(s)	2035
PciI	ACATGT	2 cut(s)	876, 2381
PciSI	GCTCTTC	1 cut(s)	2259
PctI	GAATGC	1 cut(s)	1684
PdmI	GAANNNNTTC	1 cut(s)	1455
PfeI	GAWTC	7 cut(s)	241, 840, 1052, 1400, 1451, 1572, 1849
PflMI	CCANNNNNTGG	1 cut(s)	750
PkrI	GCNGC	6 cut(s)	583, 610, 1123, 1156, 1533, 2436
PleI	GAGTC	1 cut(s)	517
PpsI	GAGTC	1 cut(s)	517
Ppu21I	YACGTR	1 cut(s)	1093
PpuMI	RGGWCCY	1 cut(s)	2097
PscI	ACATGT	2 cut(s)	876, 2381
PshBI	ATTAAT	1 cut(s)	111
Psp1406I	AACGTT	2 cut(s)	1230, 2179
Psp5II	RGGWCCY	1 cut(s)	2097
Psp6I	CCWGG	4 cut(s)	259, 409, 1788, 2099
PspFI	CCCAGC	1 cut(s)	24
PspGI	CCWGG	4 cut(s)	259, 409, 1788, 2099
PspN4I	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
PspPPI	RGGWCCY	1 cut(s)	2097
PstI	CTGCAG	2 cut(s)	613, 913
PstNI	CAGNNNCTG	1 cut(s)	1861
PsuI	RGATCY	2 cut(s)	857, 2476
PvuII	CAGCTG	3 cut(s)	67, 1154, 1499
RseI	CAYNNNNRTG	2 cut(s)	230, 1864
SapI	GCTCTTC	1 cut(s)	2259
SaqAI	TTAA	7 cut(s)	111, 306, 998, 1020, 1440, 2105, 2325
SatI	GCNGC	6 cut(s)	582, 609, 1122, 1155, 1532, 2435
Sau3AI	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
ScaI	AGTACT	2 cut(s)	163, 1689
SchI	GAGTC	1 cut(s)	518
ScrFI	CCNGG	4 cut(s)	261, 411, 1790, 2101
SduI	GDGCHC	2 cut(s)	2192, 2527
SfaNI	GCATC	8 cut(s)	346, 658, 937, 1218, 1495, 1876, 1974, 2449
SfcI	CTRYAG	2 cut(s)	609, 909
SinI	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
SmiMI	CAYNNNNRTG	2 cut(s)	230, 1864
SmlI	CTYRAG	1 cut(s)	398
SmoI	CTYRAG	1 cut(s)	398
SphI	GCATGC	2 cut(s)	1604, 2494
SseBI	AGGCCT	1 cut(s)	2035
SsiI	CCGC	2 cut(s)	1467, 1841
SspMI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
StuI	AGGCCT	1 cut(s)	2035
StyD4I	CCNGG	4 cut(s)	259, 409, 1788, 2099
StyI	CCWWGG	6 cut(s)	514, 644, 883, 1196, 1720, 2093
TaiI	ACGT	5 cut(s)	61, 1095, 1233, 1868, 2182
TaqI	TCGA	3 cut(s)	1434, 1811, 2399
TatI	WGTACW	2 cut(s)	161, 1687
TfiI	GAWTC	7 cut(s)	241, 840, 1052, 1400, 1451, 1572, 1849
Tru1I	TTAA	7 cut(s)	111, 306, 998, 1020, 1440, 2105, 2325
Tru9I	TTAA	7 cut(s)	111, 306, 998, 1020, 1440, 2105, 2325
TseFI	GTSAC	2 cut(s)	1162, 2384
TseI	GCWGC	6 cut(s)	581, 608, 1121, 1154, 1531, 2434
Tsp45I	GTSAC	2 cut(s)	1162, 2384
TspGWI	ACGGA	2 cut(s)	1690, 2315
Van91I	CCANNNNNTGG	1 cut(s)	750
VpaK11BI	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
VspI	ATTAAT	1 cut(s)	111
XapI	RAATTY	5 cut(s)	126, 373, 461, 782, 2365
XceI	RCATGY	5 cut(s)	880, 1517, 1604, 2385, 2494
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1813, 2076, 2470
XmaJI	CCTAGG	1 cut(s)	2093
XmiI	GTMKAC	1 cut(s)	1236
XmnI	GAANNNNTTC	1 cut(s)	1455
XspI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
ZrmI	AGTACT	2 cut(s)	163, 1689
Zsp2I	ATGCAT	2 cut(s)	595, 1515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.