MD10G1067800.v1.1

Galactose binding lectin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001965
Physical Location & Seq
Reverse (-)
9328615 .. 9334732
6118 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1067800.v1.1.491

Sequence Viewer

Length: 2547 bp
ATGGGGGCTAATAGCTCAGCTTTGAGCTGGGTTCTGCTCTGCTGCTGCATAGTGTGGTCTTCTGTGTATGTTGAGCAGACAGAGTGCAATGTTGTGTTTGATAGGAAGGCTCTTGTCATTGATGGGCAGAGGAGACTTCTCTTCTCTGGCTCCATTCACTATCCCAGAAGTACTCCTGAGATGTGGGAAGGTCTTATACAGAAGGCCAAAGATGGTGGCTTGGATGCCATTGACACCTATGTCTTCTGGAATCTTCATGAACCTTCTCCTGGCAATTATAATTTTGAGGGGAGGAACGATTTGGTTCGGTTCATAAAGACGGTTCACAAGGCAGGGCTCTATGTTCATCTTCGGATTGGTCCTTATATTTGTTCAGAATGGAATTTTGGGGGGTTTCCAGTTTGGCTGAAGTTTGTTCCAGGCATTAGCTTCAGAACAGATAATGAGCCTTTTAAGTCAGCAATGCAAAAATTTACACAGAAAGTTGTCCAGTTGATGAAGAACGAAAAGTTGTTTGAGTCACAAGGTGGCCCCATTATCCTTTCTCAGATTGAGAACGAATACGAGCCAGAAAGCAAGGCGTTTGGGGCTTCTGGTTATGCATACATGACCTGGGCTGCAAAGATGGCTGTTGGAATGGGTACCGGGGTCCCATGGGTCATGTGCAAGGAAGATGATGCCCCTGACCCAGTGATAAACACTTGCAATGGTTTTTATTGCGATTATTTTTCTCCCAACAAACCATACAAACCCACAATGTGGACTGAAGCTTGGAGTGGCTGGTTCACAGAATTTGGTGGCCCAATTTACCAGCGGCCTGTTGAAGATTTGGCATTTGCAGTTGCCAGATTCATCCAAAAAGGAGGCTCCTTCATAAATTATTACATGTACCATGGAGGGACCAACTTTGGAAGAACTGCTGGAGGCCCCTTTATAACTACCAGCTATGACTATGATGCTCCCATCGACGAATACGGCTTGATCAGGCAACCTAAGTATGGCCACTTAAAGGAACTCCATAAGGCTGTTAAGCTATGTGAGCTAGCTCTGCTGAATGCTGATCCTACTGTCACAACCTTAGGGAGCTACGAACAGGCACATGTATTCTCTTCCAAGTCAGGAGGCTGTGCAGCTTTTCTCTCAAATTTCAATACAAAGTCAGCTGCAAAAGTGACTTTCAATAACATGAACTTTCACCTCCCCCCTTGGTCCATCAGCATCCTTCCAGATTGCAAAAATGTTGCATTCAACACTGCCCGAGTGAGAGTTCAGACATTCCAAACACAACTTTTGCCCACTAACTCTGAGTTGCACTCGTGGGGAATCTTCAATGAAGATGTTTCTTCAGTGGCTGGTGATACTACAATCACGGTCGTTGGCCTCTTAGATCAACTGAACATTACAAGAGACTCCAGTGATTATTTGTGGTACACAACCAGTGTTGACATAGATCCATCAGAATCATTTCTACGCGGAGGTCAACATCCCAGTCTAACCGTGCAGTCAGCTGGAGATGCTATGCATGTCTTCATCAATGACCAGCTCTCAGGATCGGCTTCTGGGACTAGGGAACACAGAAGATTCACTTTTACAGGAAATGTCAACCTGCATGCTGGACTGAATAAAATTTCACTTCTCAGTATAGCTGTTGGATTGGCGAATAATGGTCCTCACTTTGAGACGCGGAACACAGGAGTGCTGGGACCAGTTGCTCTACATGGACTAGACCATGGAACAAGAGATTTATCTTGGCAGCAATGGTCATATCAGGTTGGGCTAAAAGGTGAAGCCTCGAATCTGGATTCTCCAAATTCCATTTCGGCTGTGGATTGGATGACAGGGTCCTTAGTGGCACAAAAACAACAGCCACTGACATGGTATAAGGCATACTTTGATGAACCAAATGGAGATGAGCCCCTGGCTTTGGACATGGGTAGTATGGGGAAGGGCCAAGCATGGATCAACGGGCAGAGCATCGGAAGATACTGGACTATTTACGCTGATGGCGATTGCAGTGCATGCACGTATTCTGGTACATTCAGGCCTAAGAAATGCCAATTTGGTTGTCAGCATCCAACTCAACAATGGTACCACGTTCCTCGCTCTTGGCTGAAGCCGTCTAAGAACCTCTTGGTAGTATTTGAGGAGATTGGTGGTGATGTATCAAAGGTCGCTCTTGTGAAAAAATCAGTGACGAGTGTTTGTGCCGAGGTTTCTGAAAATCACCCACACATTACAAATTGGCACACTGAGAGCCATGGCCAAACGGAAGTGCAACAGAAGCCCGAAATTGGCCTACACTGTACAGATGGACACTCCATTTCTGCTATTAAATTTTCAAGCTTTGGAACTCCATCCGGAAGCTGTGGGAAATTTCAGCATGGCACCTGTCACGCTCCAAACTCAAATGCTGTCCTGCAGAAGGAGTGCCTAGGTAAGCAGAAGTGTTCAGTGACCATATCAAACACCAACTTCGGCGCAGACCCTTGTCCAAGCAAACTAAAAAAGTTATCTGTGGAAGCTGTTTGTTCCCCTATAAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

849

Amino Acids

93.77

Weight (kDa)

6.45

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 38 - 342 1.5e-123 Glycosyl hydrolases family 35
GHD PF17834 365 - 421 4e-25 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 474 - 541 9.5e-06 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 624 - 709 3.7e-27 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 766 - 843 5.6e-21 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 279, 935
AasI GACNNNNNNGTC 1 cut(s) 239
Acc36I ACCTGC 1 cut(s) 1614
Acc65I GGTACC 2 cut(s) 641, 2088
AccB1I GGYRCC 3 cut(s) 641, 2088, 2384
AccB7I CCANNNNNTGG 1 cut(s) 759
AccII CGCG 2 cut(s) 1473, 1684
AccIII TCCGGA 1 cut(s) 2357
AciI CCGC 3 cut(s) 814, 1473, 1684
AclWI GGATC 4 cut(s) 1055, 1445, 1558, 1967
AcoI YGGCCR 2 cut(s) 1000, 2260
AcsI RAATTY 8 cut(s) 382, 470, 791, 1144, 1626, 1810, 2333, 2372
AcuI CTGAAG 5 cut(s) 415, 428, 786, 1329, 2132
AdeI CACNNNGTG 2 cut(s) 527, 759
AfaI GTAC 7 cut(s) 172, 643, 890, 1430, 2035, 2090, 2305
AfiI CCNNNNNNNGG 7 cut(s) 269, 759, 998, 1009, 1924, 2291, 2422
AflIII ACRYGT 2 cut(s) 885, 1099
AgsI TTSAA 6 cut(s) 824, 1150, 1180, 1249, 1330, 2340
AjnI CCWGG 4 cut(s) 268, 418, 611, 1917
AjuI GAANNNNNNNTTGG 4 cut(s) 369, 401, 1801, 1833
AleI CACNNNNGTG 1 cut(s) 1694
Alw26I GTCTC 3 cut(s) 127, 1401, 1673
AlwI GGATC 4 cut(s) 1055, 1445, 1558, 1967
AlwNI CAGNNNCTG 2 cut(s) 1352, 1870
Ama87I CYCGRG 1 cut(s) 1257
Aor13HI TCCGGA 1 cut(s) 2357
ApeKI GCWGC 6 cut(s) 42, 45, 617, 1130, 1163, 1753
ApoI RAATTY 8 cut(s) 382, 470, 791, 1144, 1626, 1810, 2333, 2372
Asp700I GAANNNNTTC 1 cut(s) 1464
Asp718I GGTACC 2 cut(s) 641, 2088
AspA2I CCTAGG 1 cut(s) 2431
AspLEI GCGC 1 cut(s) 2480
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 5 cut(s) 1187, 1367, 1796, 2168, 2216
AsuNHI GCTAGC 1 cut(s) 1042
AvaI CYCGRG 1 cut(s) 1257
AvaII GGWCC 7 cut(s) 359, 649, 900, 1209, 1667, 1703, 1842
AvrII CCTAGG 1 cut(s) 2431
AxyI CCTNAGG 1 cut(s) 1078
BaeI ACNNNNGTAYC 4 cut(s) 633, 666, 2072, 2105
BalI TGGCCA 2 cut(s) 1002, 2262
BanI GGYRCC 3 cut(s) 641, 2088, 2384
BanII GRGCYC 2 cut(s) 339, 1917
BarI GAAGNNNNNNTAC 2 cut(s) 180, 212
BauI CACGAG 1 cut(s) 1315
BbsI GAAGAC 3 cut(s) 51, 235, 1519
BbvI GCAGC 6 cut(s) 29, 32, 604, 1142, 1150, 1765
BccI CCATC 9 cut(s) 116, 206, 619, 971, 1220, 1462, 1997, 2303, 2362
BceAI ACGGC 2 cut(s) 991, 2101
BcgI CGANNNNNNTGC 2 cut(s) 1997, 2031
BciT130I CCWGG 4 cut(s) 270, 420, 613, 1919
BclI TGATCA 1 cut(s) 981
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 3 cut(s) 127, 1401, 1673
BfaI CTAG 4 cut(s) 1043, 1566, 1724, 2432
BfmI CTRYAG 1 cut(s) 2417
BfuAI ACCTGC 1 cut(s) 1614
BisI GCNGC 7 cut(s) 43, 46, 618, 815, 1131, 1164, 1754
BlnI CCTAGG 1 cut(s) 2431
BlpI GCTNAGC 1 cut(s) 16
BlsI GCNGC 7 cut(s) 44, 47, 619, 816, 1132, 1165, 1755
BmcAI AGTACT 1 cut(s) 172
Bme1390I CCNGG 5 cut(s) 270, 420, 613, 646, 1919
Bme18I GGWCC 7 cut(s) 359, 649, 900, 1209, 1667, 1703, 1842
BmeT110I CYCGRG 1 cut(s) 1257
BmrFI CCNGG 5 cut(s) 270, 420, 613, 646, 1919
BmrI ACTGGG 2 cut(s) 683, 1482
BmsI GCATC 7 cut(s) 214, 667, 946, 1227, 1504, 1983, 2080
BmtI GCTAGC 1 cut(s) 1046
BmuI ACTGGG 2 cut(s) 683, 1482
BoxI GACNNNNGTC 1 cut(s) 2487
BpiI GAAGAC 3 cut(s) 51, 235, 1519
BplI GAGNNNNNCTC 2 cut(s) 1298, 1330
BpmI CTGGAG 3 cut(s) 942, 1396, 1530
Bpu1102I GCTNAGC 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 646
BsaAI YACGTR 1 cut(s) 2025
BsaWI WCCGGW 1 cut(s) 2357
Bsc4I CCNNNNNNNGG 7 cut(s) 269, 759, 998, 1009, 1924, 2291, 2422
Bse1I ACTGG 8 cut(s) 398, 490, 689, 1413, 1437, 1488, 1706, 1991
Bse21I CCTNAGG 1 cut(s) 1078
Bse3DI GCAATG 4 cut(s) 94, 468, 712, 1763
BseAI TCCGGA 1 cut(s) 2357
BseBI CCWGG 4 cut(s) 270, 420, 613, 1919
BseGI GGATG 7 cut(s) 229, 852, 1218, 1483, 1839, 2071, 2354
BseLI CCNNNNNNNGG 7 cut(s) 269, 759, 998, 1009, 1924, 2291, 2422
BseMI GCAATG 4 cut(s) 94, 468, 712, 1763
BseMII CTCAG 7 cut(s) 30, 168, 560, 1296, 1560, 1651, 2241
BseNI ACTGG 8 cut(s) 398, 490, 689, 1413, 1437, 1488, 1706, 1991
BseRI GAGGAG 2 cut(s) 145, 2159
BseXI GCAGC 6 cut(s) 29, 32, 604, 1142, 1150, 1765
BseYI CCCAGC 2 cut(s) 27, 1699
BsgI GTGCAG 2 cut(s) 1149, 1520
Bsh1236I CGCG 2 cut(s) 1473, 1684
Bsh1285I CGRYCG 1 cut(s) 1374
BshNI GGYRCC 3 cut(s) 641, 2088, 2384
BsiEI CGRYCG 1 cut(s) 1374
BsiHKCI CYCGRG 1 cut(s) 1257
BsiSI CCGG 2 cut(s) 645, 2358
BslFI GGGAC 4 cut(s) 635, 913, 1576, 1716
BslI CCNNNNNNNGG 7 cut(s) 269, 759, 998, 1009, 1924, 2291, 2422
BsmAI GTCTC 3 cut(s) 127, 1401, 1673
BsmBI CGTCTC 1 cut(s) 1673
BsmFI GGGAC 4 cut(s) 635, 913, 1576, 1716
BsmI GAATGC 2 cut(s) 1060, 1244
BsoBI CYCGRG 1 cut(s) 1257
Bsp1286I GDGCHC 2 cut(s) 339, 1917
Bsp13I TCCGGA 1 cut(s) 2357
Bsp1407I TGTACA 1 cut(s) 2303
Bsp143I GATC 6 cut(s) 981, 1060, 1387, 1450, 1550, 1959
Bsp1720I GCTNAGC 1 cut(s) 16
Bsp19I CCATGG 4 cut(s) 653, 892, 1729, 2257
BspACI CCGC 3 cut(s) 814, 1473, 1684
BspCNI CTCAG 7 cut(s) 29, 169, 559, 1297, 1559, 1650, 2242
BspEI TCCGGA 1 cut(s) 2357
BspFNI CGCG 2 cut(s) 1473, 1684
BspHI TCATGA 1 cut(s) 256
BspMAI CTGCAG 1 cut(s) 2421
BspMI ACCTGC 1 cut(s) 1614
BspOI GCTAGC 1 cut(s) 1046
BspPI GGATC 4 cut(s) 1055, 1445, 1558, 1967
BspT107I GGYRCC 3 cut(s) 641, 2088, 2384
BsrDI GCAATG 4 cut(s) 94, 468, 712, 1763
BsrGI TGTACA 1 cut(s) 2303
BsrI ACTGG 8 cut(s) 398, 490, 689, 1413, 1437, 1488, 1706, 1991
BssMI GATC 6 cut(s) 981, 1060, 1387, 1450, 1550, 1959
BssSI CACGAG 1 cut(s) 1315
BssT1I CCWWGG 6 cut(s) 653, 892, 1205, 1729, 2257, 2431
Bst2BI CACGAG 1 cut(s) 1315
Bst2UI CCWGG 4 cut(s) 270, 420, 613, 1919
Bst4CI ACNGT 5 cut(s) 322, 1069, 1372, 1498, 2303
Bst6I CTCTTC 2 cut(s) 146, 1114
BstAPI GCANNNNNTGC 1 cut(s) 2019
BstAUI TGTACA 1 cut(s) 2303
BstBAI YACGTR 1 cut(s) 2025
BstC8I GCNNGC 3 cut(s) 1044, 1611, 2020
BstDSI CCRYGG 4 cut(s) 653, 892, 1729, 2257
BstENI CCTNNNNNAGG 1 cut(s) 2420
BstF5I GGATG 7 cut(s) 229, 852, 1218, 1483, 1839, 2071, 2354
BstFNI CGCG 2 cut(s) 1473, 1684
BstHHI GCGC 1 cut(s) 2480
BstKTI GATC 6 cut(s) 984, 1063, 1390, 1453, 1553, 1962
BstMAI GTCTC 3 cut(s) 127, 1401, 1673
BstMBI GATC 6 cut(s) 981, 1060, 1387, 1450, 1550, 1959
BstMCI CGRYCG 1 cut(s) 1374
BstMWI GCNNNNNNNGC 7 cut(s) 587, 626, 1039, 1048, 1514, 2019, 2281
BstNI CCWGG 4 cut(s) 270, 420, 613, 1919
BstNSI RCATGY 5 cut(s) 889, 1103, 1526, 1613, 2022
BstPAI GACNNNNGTC 1 cut(s) 2487
BstSCI CCNGG 5 cut(s) 268, 418, 611, 644, 1917
BstSFI CTRYAG 1 cut(s) 2417
BstUI CGCG 2 cut(s) 1473, 1684
BstV1I GCAGC 6 cut(s) 29, 32, 604, 1142, 1150, 1765
BstV2I GAAGAC 3 cut(s) 51, 235, 1519
BstX2I RGATCY 1 cut(s) 1450
BstXI CCANNNNNNTGG 1 cut(s) 1875
BstYI RGATCY 1 cut(s) 1450
Bsu36I CCTNAGG 1 cut(s) 1078
BtgI CCRYGG 4 cut(s) 653, 892, 1729, 2257
BtsCI GGATG 7 cut(s) 229, 852, 1218, 1483, 1839, 2071, 2354
BtsI GCAGTG 2 cut(s) 1251, 2020
BveI ACCTGC 1 cut(s) 1614
Cac8I GCNNGC 3 cut(s) 1044, 1611, 2020
CaiI CAGNNNCTG 2 cut(s) 1352, 1870
CciI TCATGA 1 cut(s) 256
CfoI GCGC 1 cut(s) 2480
CseI GACGC 1 cut(s) 1690
Csp6I GTAC 7 cut(s) 171, 642, 889, 1429, 2034, 2089, 2304
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviQI GTAC 7 cut(s) 171, 642, 889, 1429, 2034, 2089, 2304
DpnI GATC 6 cut(s) 983, 1062, 1389, 1452, 1552, 1961
DpnII GATC 6 cut(s) 981, 1060, 1387, 1450, 1550, 1959
DraIII CACNNNGTG 2 cut(s) 527, 759
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 2 cut(s) 1000, 2260
Eam1104I CTCTTC 2 cut(s) 146, 1114
EarI CTCTTC 2 cut(s) 146, 1114
Eco130I CCWWGG 6 cut(s) 653, 892, 1205, 1729, 2257, 2431
Eco147I AGGCCT 1 cut(s) 2044
Eco24I GRGCYC 2 cut(s) 339, 1917
Eco47I GGWCC 7 cut(s) 359, 649, 900, 1209, 1667, 1703, 1842
Eco57I CTGAAG 5 cut(s) 415, 428, 786, 1329, 2132
Eco81I CCTNAGG 1 cut(s) 1078
Eco88I CYCGRG 1 cut(s) 1257
EcoNI CCTNNNNNAGG 1 cut(s) 2420
EcoO109I RGGNCCY 3 cut(s) 649, 926, 1842
EcoRII CCWGG 4 cut(s) 268, 418, 611, 1917
EcoT14I CCWWGG 6 cut(s) 653, 892, 1205, 1729, 2257, 2431
EcoT22I ATGCAT 2 cut(s) 604, 1524
EcoT38I GRGCYC 2 cut(s) 339, 1917
ErhI CCWWGG 6 cut(s) 653, 892, 1205, 1729, 2257, 2431
Esp3I CGTCTC 1 cut(s) 1673
FalI AAGNNNNNCTT 6 cut(s) 1570, 1602, 1874, 1906, 2114, 2146
FaqI GGGAC 4 cut(s) 635, 913, 1576, 1716
FbaI TGATCA 1 cut(s) 981
Fnu4HI GCNGC 7 cut(s) 43, 46, 618, 815, 1131, 1164, 1754
FokI GGATG 7 cut(s) 236, 839, 1205, 1470, 1846, 2058, 2341
FriOI GRGCYC 2 cut(s) 339, 1917
Fsp4HI GCNGC 7 cut(s) 43, 46, 618, 815, 1131, 1164, 1754
FspBI CTAG 4 cut(s) 1043, 1566, 1724, 2432
GlaI GCGC 1 cut(s) 2479
GluI GCNGC 7 cut(s) 43, 46, 618, 815, 1131, 1164, 1754
GsaI CCCAGC 2 cut(s) 31, 1703
GsuI CTGGAG 3 cut(s) 942, 1396, 1530
HapII CCGG 2 cut(s) 645, 2358
HgaI GACGC 1 cut(s) 1690
HhaI GCGC 1 cut(s) 2480
Hin6I GCGC 1 cut(s) 2478
HinP1I GCGC 1 cut(s) 2478
HincII GTYRAC 3 cut(s) 1444, 1481, 1603
HindII GTYRAC 3 cut(s) 1444, 1481, 1603
HindIII AAGCTT 2 cut(s) 768, 2341
HinfI GANTC 9 cut(s) 250, 518, 849, 1323, 1409, 1460, 1581, 1795, 1802
HpaII CCGG 2 cut(s) 645, 2358
HphI GGTGA 5 cut(s) 1187, 1367, 1796, 2168, 2216
Hpy166II GTNNAC 7 cut(s) 325, 762, 786, 1431, 1444, 1481, 1603
Hpy188I TCNGA 9 cut(s) 354, 376, 434, 549, 1272, 1306, 1459, 1979, 2218
Hpy188III TCNNGA 8 cut(s) 176, 247, 257, 1119, 1226, 1548, 1799, 2358
Hpy8I GTNNAC 7 cut(s) 325, 762, 786, 1431, 1444, 1481, 1603
Hpy99I CGWCG 1 cut(s) 971
HpyAV CCTTC 8 cut(s) 100, 182, 196, 273, 880, 1232, 1939, 2416
HpyCH4III ACNGT 5 cut(s) 322, 1069, 1372, 1498, 2303
HpyCH4IV ACGT 2 cut(s) 2024, 2094
HpyF10VI GCNNNNNNNGC 7 cut(s) 587, 626, 1039, 1048, 1514, 2019, 2281
HpySE526I ACGT 2 cut(s) 2024, 2094
HspAI GCGC 1 cut(s) 2478
KflI GGGWCCC 1 cut(s) 649
Kpn2I TCCGGA 1 cut(s) 2357
KpnI GGTACC 2 cut(s) 645, 2092
Ksp22I TGATCA 1 cut(s) 981
Kzo9I GATC 6 cut(s) 981, 1060, 1387, 1450, 1550, 1959
LmnI GCTCC 5 cut(s) 155, 872, 964, 1083, 2401
Lsp1109I GCAGC 6 cut(s) 29, 32, 604, 1142, 1150, 1765
LweI GCATC 7 cut(s) 214, 667, 946, 1227, 1504, 1983, 2080
MaeI CTAG 4 cut(s) 1043, 1566, 1724, 2432
MaeII ACGT 2 cut(s) 2024, 2094
MaeIII GTNAC 6 cut(s) 519, 1069, 1171, 2191, 2390, 2452
MalI GATC 6 cut(s) 983, 1062, 1389, 1452, 1552, 1961
MboI GATC 6 cut(s) 981, 1060, 1387, 1450, 1550, 1959
MflI RGATCY 1 cut(s) 1450
MhlI GDGCHC 2 cut(s) 339, 1917
MlsI TGGCCA 2 cut(s) 1002, 2262
MluNI TGGCCA 2 cut(s) 1002, 2262
MlyI GAGTC 2 cut(s) 527, 1403
MmeI TCCRAC 3 cut(s) 613, 1630, 2099
Mox20I TGGCCA 2 cut(s) 1002, 2262
Mph1103I ATGCAT 2 cut(s) 604, 1524
MroI TCCGGA 1 cut(s) 2357
MroXI GAANNNNTTC 1 cut(s) 1464
MscI TGGCCA 2 cut(s) 1002, 2262
MseI TTAA 4 cut(s) 453, 1007, 1029, 2331
MslI CAYNNNNRTG 2 cut(s) 1694, 1873
Msp20I TGGCCA 2 cut(s) 1002, 2262
MspA1I CMGCKG 3 cut(s) 814, 1163, 1508
MspI CCGG 2 cut(s) 645, 2358
MspR9I CCNGG 5 cut(s) 270, 420, 613, 646, 1919
Mva1269I GAATGC 2 cut(s) 1060, 1244
MvaI CCWGG 4 cut(s) 270, 420, 613, 1919
MvnI CGCG 2 cut(s) 1473, 1684
MwoI GCNNNNNNNGC 7 cut(s) 587, 626, 1039, 1048, 1514, 2019, 2281
NciI CCSGG 1 cut(s) 646
NcoI CCATGG 4 cut(s) 653, 892, 1729, 2257
NdeII GATC 6 cut(s) 981, 1060, 1387, 1450, 1550, 1959
NheI GCTAGC 1 cut(s) 1042
NmeAIII GCCGAG 1 cut(s) 2233
NmuCI GTSAC 6 cut(s) 519, 1069, 1171, 2191, 2390, 2452
NsiI ATGCAT 2 cut(s) 604, 1524
NspI RCATGY 5 cut(s) 889, 1103, 1526, 1613, 2022
OliI CACNNNNGTG 1 cut(s) 1694
PaeI GCATGC 2 cut(s) 1613, 2022
PagI TCATGA 1 cut(s) 256
PceI AGGCCT 1 cut(s) 2044
PciI ACATGT 2 cut(s) 885, 1099
PcsI WCGNNNNNNNCGW 1 cut(s) 2004
PctI GAATGC 2 cut(s) 1060, 1244
PdmI GAANNNNTTC 1 cut(s) 1464
PfeI GAWTC 7 cut(s) 250, 849, 1323, 1460, 1581, 1795, 1802
PflFI GACNNNGTC 1 cut(s) 1840
PflMI CCANNNNNTGG 1 cut(s) 759
PkrI GCNGC 7 cut(s) 44, 47, 619, 816, 1132, 1165, 1755
PleI GAGTC 2 cut(s) 526, 1403
PpsI GAGTC 2 cut(s) 526, 1403
Ppu21I YACGTR 1 cut(s) 2025
PpuMI RGGWCCY 2 cut(s) 649, 1842
PscI ACATGT 2 cut(s) 885, 1099
PshAI GACNNNNGTC 1 cut(s) 2487
PsiI TTATAA 2 cut(s) 279, 935
Psp5II RGGWCCY 2 cut(s) 649, 1842
Psp6I CCWGG 4 cut(s) 268, 418, 611, 1917
PspFI CCCAGC 2 cut(s) 27, 1699
PspGI CCWGG 4 cut(s) 268, 418, 611, 1917
PspPPI RGGWCCY 2 cut(s) 649, 1842
PstI CTGCAG 1 cut(s) 2421
PstNI CAGNNNCTG 2 cut(s) 1352, 1870
PsuI RGATCY 1 cut(s) 1450
PsyI GACNNNGTC 1 cut(s) 1840
PvuII CAGCTG 2 cut(s) 1163, 1508
RsaI GTAC 7 cut(s) 172, 643, 890, 1430, 2035, 2090, 2305
RsaNI GTAC 7 cut(s) 171, 642, 889, 1429, 2034, 2089, 2304
RseI CAYNNNNRTG 2 cut(s) 1694, 1873
SaqAI TTAA 4 cut(s) 453, 1007, 1029, 2331
SatI GCNGC 7 cut(s) 43, 46, 618, 815, 1131, 1164, 1754
Sau3AI GATC 6 cut(s) 981, 1060, 1387, 1450, 1550, 1959
ScaI AGTACT 1 cut(s) 172
SchI GAGTC 2 cut(s) 527, 1403
ScrFI CCNGG 5 cut(s) 270, 420, 613, 646, 1919
SduI GDGCHC 2 cut(s) 339, 1917
SfaNI GCATC 7 cut(s) 214, 667, 946, 1227, 1504, 1983, 2080
SfcI CTRYAG 1 cut(s) 2417
SinI GGWCC 7 cut(s) 359, 649, 900, 1209, 1667, 1703, 1842
SmiMI CAYNNNNRTG 2 cut(s) 1694, 1873
SphI GCATGC 2 cut(s) 1613, 2022
SseBI AGGCCT 1 cut(s) 2044
SsiI CCGC 3 cut(s) 814, 1473, 1684
SspMI CTAG 4 cut(s) 1043, 1566, 1724, 2432
StuI AGGCCT 1 cut(s) 2044
StyD4I CCNGG 5 cut(s) 268, 418, 611, 644, 1917
StyI CCWWGG 6 cut(s) 653, 892, 1205, 1729, 2257, 2431
TaaI ACNGT 5 cut(s) 322, 1069, 1372, 1498, 2303
TaiI ACGT 2 cut(s) 2027, 2097
TaqI TCGA 2 cut(s) 966, 1793
TatI WGTACW 2 cut(s) 170, 2303
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 7 cut(s) 250, 849, 1323, 1460, 1581, 1795, 1802
Tru1I TTAA 4 cut(s) 453, 1007, 1029, 2331
Tru9I TTAA 4 cut(s) 453, 1007, 1029, 2331
TseFI GTSAC 6 cut(s) 519, 1069, 1171, 2191, 2390, 2452
TseI GCWGC 6 cut(s) 42, 45, 617, 1130, 1163, 1753
Tsp45I GTSAC 6 cut(s) 519, 1069, 1171, 2191, 2390, 2452
TspGWI ACGGA 1 cut(s) 2282
Tth111I GACNNNGTC 1 cut(s) 1840
Van91I CCANNNNNTGG 1 cut(s) 759
VpaK11BI GGWCC 7 cut(s) 359, 649, 900, 1209, 1667, 1703, 1842
XagI CCTNNNNNAGG 1 cut(s) 2420
XapI RAATTY 8 cut(s) 382, 470, 791, 1144, 1626, 1810, 2333, 2372
XceI RCATGY 5 cut(s) 889, 1103, 1526, 1613, 2022
XcmI CCANNNNNNNNNTGG 2 cut(s) 1822, 2082
XmaJI CCTAGG 1 cut(s) 2431
XmnI GAANNNNTTC 1 cut(s) 1464
XspI CTAG 4 cut(s) 1043, 1566, 1724, 2432
ZrmI AGTACT 1 cut(s) 172
Zsp2I ATGCAT 2 cut(s) 604, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.