MD15G1251100.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001965
Physical Location & Seq
Reverse (-)
21133993 .. 21139636
5644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1251100.v1.1.491

Sequence Viewer

Length: 2562 bp
ATGGAACCAAACTCAGCTTCCAAGCTGGGTTTTTTCATGGGGTTGTTTTTGCTGCTGGGTTTTCAGCTGGTTCACTGTGCTGTCACATATGACAGGAAGGCCATTGTGATTAATGGGCAGAGACGAATCCTAATTTCTGGGTCTATACATTACCCCAGAAGCACTCCTGAGGTGTGGGAAGATCTGATTCAGAAGGCAAAAGATGGAGGGCTTGATGTGGTTGAAACCTATGTGTTCTGGAATGTGCATGAGCCTACTCCTGGCAATTACAATTTTGAAGGGAGGTATGATCTGGTGAGGTTCCTCAAGACCATACAGAAGGCAGGGCTCTATGCTCACCTTCGCATTGGCCCTTATGTTTGCGCTGAGTGGAACTTTGGGGGTTTTCCTGTTTGGCTGAAGTATGTTCCAGGCATCAGCTTCAGGACAGACAATGAACCTTTCAAGAGGGCAATGCAAGGGTTCACACAAAAGATTGTGGGACTGATGAAGAGTGAAAGTTTGTTTGAGTCCCAGGGTGGTCCCATCATACTCTCTCAGATTGAGAATGAGTATGGAGCGCAGAGTAAGTTATTTGGAGCCGCTGGCCATAATTACATTACTTGGGCCGCAGAAATGGCGGTTGGGTTGGACACCGGAGTGCCATGGGTGATGTGCAAGGAAGAAGATGCTCCAGATCCAGTGATAAACACTTGTAACGGTTTCTACTGCGATTCTTTCTCTCCCAACAAACCCTATAAGCCTACGATTTGGACAGAGACATGGAGTGGCTGGTTTACGGAGTTTGGCGGTCCAATTCACCAACGACCAGTTCAGGATTTAGCATATGCAGTTGCGACATTCATACAGAAAGGAGGATCATTTGTTAACTATTACATGTACCATGGGGGTACCAATTTTGGACGCACAGCCGGGGGCCCTTTCATCACTACCAGCTACGATTATGATGCTCCATTAGATGAATATGGTTTAATCAGGCAGCCAAAGTATGGTCACCTCAAGGAGCTTCACAAGGCTATTAAGATGTGTGAACGGGCTTTAGTATCAGCGGATCCAATCATAACTTCACTGGGGAATTTTCAACAGGCTTATGTGTACACTTCCGAATCAGGAGATTGCTCTGCATTTCTTTCAAACCATGATTCCAAGTCAGCTGCGAGAGTGATGTTTAATAACATGCACTACAACCTGCCTCCTTGGTCCATCAGTATCCTTCCTGATTGCAGAAATGTAGTCTTTAACACTGCAAAGGTTGGAGTTCAAACGTCACAAATGCAAATGTTGCCAACAAATATTCCGATGCTATCATGGGAGAGTTATGATGAAGATCTTACTTCTCTGGATGACAGCTCAACAATGACCGCTCCTGGTCTATTGGAACAGATAAATGTCACTAGGGATAGTACTGACTACCTCTGGTACATAACTAGCGTTGATATTGATTCATCTGAGTCCTTCCTGCATGGAGGGGAACTCCCGACCCTTATAGTTCAATCAACAGGCCATGCTGTGCACATTTTCATTAATGGACAGCTTACAGGTTCTGCCTTTGGAACAAGGGTGAGTAGGAGATTCACATATACTGGCAAGGTCAATCTACGTGCCGGAACAAACAGAATTGCACTGCTGAGTGTTGCCGTCGGATTACCTAATGTGGGCGGACACTTTGAGGCTTGGAATACTGGAATCCTCGGCCCCGTTGTACTTCATGGACTTAACCAGGGAAAATGGGACTTATCTTGGCAGAAGTGGACCTACCAGGTCGGACTGAAAGGAGAAGCCATGAATCTTGCGTCTCCAAATGCTTTTTCCTCTGTCGAATGGATAGGCGGATCATTAATTGCACAAAAACAGCAGCCATTGACTTGGCACAAGACTATTTTCAATGAACCCGAAGGAAGTGAGCCATTGGCTTTGGACATGGAGGGAATGGGAAAAGGCCAAATATGGATTAATGGGCAGAGTATTGGGAGATATTGGACAGCATTTGCGAATGGTAATTGCAATGGGTGCAGTTATGCTGGAGGGTTTAGGCCTACAAAGTGTCAATTGGGTTGCGGCAAACCCACACAACGATATTATCATGTGCCTCGGTCTTGGTTAAAGCCAACTCAAAATCTGTTGGTACTTTTTGAGGAACTTGGAGGGGATCCCTCTAGAATATCTCTTGTAAAGAGAGCTGTGAGCAGTGTTTGCTCTGAAGTTGCTGAATACCACCCAACAATCAAGAATTGGCACATCGAAAGCTATGGAAAAGTAGAAGACTTCCACAGCCCCAAGGTTCATTTGCGCTGTAACCCAGGGCAGGCTATCTCTTCCATAAAATTCGCTAGCTTCGGAACACCCTTGGGAACCTGTGGGAGCTACCAAGAAGGAACTTGCCATGCCACCACGTCATACTCTGTATTGCAGAAGAAGTGCATAGGGAAGCAGAGATGTGCAGTCACCATATCCAACAGCAACTTTGGAGATCCATGTCCGAAGGTGTTGAAGCGGCTGTCAGTAGAAGCTGTGTGTGCTCCCATAACTTCAACAAACGTGGAACCCAATTCACGTGGTTAG

Protein Analysis

854

Amino Acids

94.53

Weight (kDa)

7.07

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 35 - 339 3e-123 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 56 - 134 8.3e-06 Beta-galactosidase
GHD PF17834 362 - 418 1.1e-26 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 471 - 538 9.5e-08 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 621 - 706 1e-24 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 763 - 839 1.4e-21 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1197
Acc65I GGTACC 1 cut(s) 890
AccB1I GGYRCC 1 cut(s) 890
AccB7I CCANNNNNTGG 1 cut(s) 989
AccBSI CCGCTC 1 cut(s) 1364
AclWI GGATC 8 cut(s) 671, 865, 1046, 1059, 1840, 2144, 2157, 2465
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 2 cut(s) 1075, 2324
AcuI CTGAAG 3 cut(s) 406, 419, 2220
AcvI CACGTG 1 cut(s) 2555
AfaI GTAC 7 cut(s) 881, 892, 1097, 1405, 1421, 1704, 2127
AfiI CCNNNNNNNGG 4 cut(s) 260, 989, 1655, 2305
AflIII ACRYGT 1 cut(s) 876
AjiI CACGTC 1 cut(s) 2394
AjnI CCWGG 7 cut(s) 259, 409, 513, 1366, 1719, 1758, 2299
AjuI GAANNNNNNNTTGG 9 cut(s) 33, 606, 638, 795, 827, 1048, 1080, 1792, 1824
AleI CACNNNNGTG 1 cut(s) 638
Alw21I GWGCWC 2 cut(s) 1515, 2521
Alw26I GTCTC 3 cut(s) 115, 752, 1800
Alw44I GTGCAC 1 cut(s) 1511
AlwI GGATC 8 cut(s) 671, 865, 1046, 1059, 1840, 2144, 2157, 2465
AlwNI CAGNNNCTG 1 cut(s) 1544
AoxI GGCC 9 cut(s) 99, 349, 586, 606, 916, 1501, 1693, 1939, 2033
ApaI GGGCCC 1 cut(s) 920
ApaLI GTGCAC 1 cut(s) 1511
ApeKI GCWGC 4 cut(s) 52, 979, 1154, 1855
ApoI RAATTY 2 cut(s) 1075, 2324
AseI ATTAAT 4 cut(s) 111, 1524, 1838, 1953
Asp718I GGTACC 1 cut(s) 890
AspLEI GCGC 3 cut(s) 365, 562, 2292
AspS9I GGNCC 9 cut(s) 350, 521, 606, 791, 916, 917, 1200, 1694, 1752
AsuC2I CCSGG 1 cut(s) 913
AsuHPI GGTGA 7 cut(s) 307, 329, 661, 791, 986, 1573, 2437
AsuNHI GCTAGC 1 cut(s) 2330
AvaII GGWCC 4 cut(s) 521, 791, 1200, 1752
AxyI CCTNAGG 1 cut(s) 168
BaeGI GKGCMC 2 cut(s) 920, 1515
BalI TGGCCA 1 cut(s) 588
BamHI GGATCC 2 cut(s) 1051, 2149
BanI GGYRCC 1 cut(s) 890
BanII GRGCYC 2 cut(s) 330, 920
BarI GAAGNNNNNNTAC 2 cut(s) 1739, 1771
BbrPI CACGTG 1 cut(s) 2555
BbsI GAAGAC 1 cut(s) 2268
Bbv12I GWGCWC 2 cut(s) 1515, 2521
BbvI GCAGC 4 cut(s) 39, 991, 1141, 1867
BccI CCATC 3 cut(s) 197, 533, 1211
BceAI ACGGC 1 cut(s) 1622
BcgI CGANNNNNNTGC 2 cut(s) 929, 963
BciT130I CCWGG 7 cut(s) 261, 411, 515, 1368, 1721, 1760, 2301
BciVI GTATCC 1 cut(s) 1220
BcnI CCSGG 1 cut(s) 913
BcoDI GTCTC 3 cut(s) 115, 752, 1800
BfaI CTAG 4 cut(s) 1395, 1428, 2157, 2331
BfuAI ACCTGC 1 cut(s) 1197
BfuI GTATCC 1 cut(s) 1220
BglII AGATCT 2 cut(s) 181, 1327
BisI GCNGC 8 cut(s) 53, 582, 609, 980, 1155, 1856, 2059, 2495
BlsI GCNGC 8 cut(s) 54, 583, 610, 981, 1156, 1857, 2060, 2496
BmcAI AGTACT 1 cut(s) 1405
Bme1390I CCNGG 8 cut(s) 261, 411, 515, 913, 1368, 1721, 1760, 2301
Bme18I GGWCC 4 cut(s) 521, 791, 1200, 1752
BmgBI CACGTC 1 cut(s) 2394
BmgT120I GGNCC 9 cut(s) 350, 521, 606, 791, 916, 917, 1200, 1694, 1752
BmrFI CCNGG 8 cut(s) 261, 411, 515, 913, 1368, 1721, 1760, 2301
BmrI ACTGGG 1 cut(s) 1079
BmsI GCATC 4 cut(s) 423, 658, 937, 1290
BmtI GCTAGC 1 cut(s) 2334
BmuI ACTGGG 1 cut(s) 1079
BpiI GAAGAC 1 cut(s) 2268
BplI GAGNNNNNCTC 2 cut(s) 1458, 1490
BpmI CTGGAG 2 cut(s) 657, 2043
BpuEI CTTGAG 2 cut(s) 290, 983
BpuMI CCSGG 1 cut(s) 913
BsaAI YACGTR 2 cut(s) 1601, 2555
BsaBI GATNNNNATC 1 cut(s) 1326
BsaWI WCCGGW 1 cut(s) 635
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 989, 1655, 2305
Bse1I ACTGG 5 cut(s) 680, 809, 1074, 1588, 1687
Bse21I CCTNAGG 1 cut(s) 168
Bse3DI GCAATG 2 cut(s) 459, 2011
Bse8I GATNNNNATC 1 cut(s) 1326
BseBI CCWGG 7 cut(s) 261, 411, 515, 1368, 1721, 1760, 2301
BseGI GGATG 1 cut(s) 1348
BseJI GATNNNNATC 1 cut(s) 1326
BseLI CCNNNNNNNGG 4 cut(s) 260, 989, 1655, 2305
BseMI GCAATG 2 cut(s) 459, 2011
BseMII CTCAG 6 cut(s) 27, 159, 357, 551, 1440, 1619
BseNI ACTGG 5 cut(s) 680, 809, 1074, 1588, 1687
BseSI GKGCMC 2 cut(s) 920, 1515
BseXI GCAGC 4 cut(s) 39, 991, 1141, 1867
BseYI CCCAGC 2 cut(s) 25, 55
BsgI GTGCAG 2 cut(s) 2032, 2460
BshFI GGCC 9 cut(s) 101, 351, 588, 608, 918, 1503, 1695, 1941, 2035
BshNI GGYRCC 1 cut(s) 890
BsiHKAI GWGCWC 2 cut(s) 1515, 2521
BsiSI CCGG 3 cut(s) 636, 912, 1605
BslFI GGGAC 4 cut(s) 495, 496, 507, 1745
BslI CCNNNNNNNGG 4 cut(s) 260, 989, 1655, 2305
BsmAI GTCTC 3 cut(s) 115, 752, 1800
BsmBI CGTCTC 2 cut(s) 115, 1800
BsmFI GGGAC 4 cut(s) 495, 496, 507, 1745
BsnI GGCC 9 cut(s) 101, 351, 588, 608, 918, 1503, 1695, 1941, 2035
Bsp120I GGGCCC 1 cut(s) 916
Bsp1286I GDGCHC 4 cut(s) 330, 920, 1515, 2521
Bsp1407I TGTACA 1 cut(s) 1095
Bsp143I GATC 9 cut(s) 181, 289, 676, 857, 1051, 1327, 1832, 2149, 2470
Bsp19I CCATGG 2 cut(s) 644, 883
BspANI GGCC 9 cut(s) 101, 351, 588, 608, 918, 1503, 1695, 1941, 2035
BspCNI CTCAG 6 cut(s) 26, 160, 358, 550, 1441, 1620
BspMI ACCTGC 1 cut(s) 1197
BspOI GCTAGC 1 cut(s) 2334
BspPI GGATC 8 cut(s) 671, 865, 1046, 1059, 1840, 2144, 2157, 2465
BspT107I GGYRCC 1 cut(s) 890
BsrBI CCGCTC 1 cut(s) 1364
BsrDI GCAATG 2 cut(s) 459, 2011
BsrGI TGTACA 1 cut(s) 1095
BsrI ACTGG 5 cut(s) 680, 809, 1074, 1588, 1687
BssMI GATC 9 cut(s) 181, 289, 676, 857, 1051, 1327, 1832, 2149, 2470
BssT1I CCWWGG 5 cut(s) 644, 883, 1196, 2277, 2346
Bst2UI CCWGG 7 cut(s) 261, 411, 515, 1368, 1721, 1760, 2301
Bst4CI ACNGT 2 cut(s) 77, 701
Bst6I CTCTTC 2 cut(s) 485, 2320
BstAPI GCANNNNNTGC 3 cut(s) 1282, 2010, 2193
BstAUI TGTACA 1 cut(s) 1095
BstBAI YACGTR 2 cut(s) 1601, 2555
BstC8I GCNNGC 3 cut(s) 586, 2307, 2332
BstDEI CTNAG 6 cut(s) 13, 168, 366, 537, 1449, 1628
BstDSI CCRYGG 2 cut(s) 644, 883
BstEII GGTNACC 1 cut(s) 992
BstF5I GGATG 1 cut(s) 1348
BstHHI GCGC 3 cut(s) 365, 562, 2292
BstKTI GATC 9 cut(s) 184, 292, 679, 860, 1054, 1330, 1835, 2152, 2473
BstMAI GTCTC 3 cut(s) 115, 752, 1800
BstMBI GATC 9 cut(s) 181, 289, 676, 857, 1051, 1327, 1832, 2149, 2470
BstMWI GCNNNNNNNGC 5 cut(s) 617, 1282, 2010, 2193, 2516
BstNI CCWGG 7 cut(s) 261, 411, 515, 1368, 1721, 1760, 2301
BstNSI RCATGY 2 cut(s) 880, 1180
BstPI GGTNACC 1 cut(s) 992
BstSCI CCNGG 8 cut(s) 259, 409, 513, 911, 1366, 1719, 1758, 2299
BstSLI GKGCMC 2 cut(s) 920, 1515
BstV1I GCAGC 4 cut(s) 39, 991, 1141, 1867
BstV2I GAAGAC 1 cut(s) 2268
BstX2I RGATCY 6 cut(s) 181, 676, 1051, 1327, 2149, 2470
BstXI CCANNNNNNTGG 1 cut(s) 1866
BstYI RGATCY 6 cut(s) 181, 676, 1051, 1327, 2149, 2470
Bsu36I CCTNAGG 1 cut(s) 168
BsuI GTATCC 1 cut(s) 1220
BsuRI GGCC 9 cut(s) 101, 351, 588, 608, 918, 1503, 1695, 1941, 2035
BtgI CCRYGG 2 cut(s) 644, 883
BtrI CACGTC 1 cut(s) 2394
BtsCI GGATG 1 cut(s) 1348
BtsI GCAGTG 3 cut(s) 1242, 1622, 2194
BtsIMutI CAGTG 6 cut(s) 73, 687, 1067, 1242, 1622, 2194
BveI ACCTGC 1 cut(s) 1197
Cac8I GCNNGC 3 cut(s) 586, 2307, 2332
CaiI CAGNNNCTG 1 cut(s) 1544
CfoI GCGC 3 cut(s) 365, 562, 2292
Cfr13I GGNCC 9 cut(s) 350, 521, 606, 791, 916, 917, 1200, 1694, 1752
CseI GACGC 2 cut(s) 912, 1782
CsiI ACCWGGT 1 cut(s) 1758
Csp6I GTAC 7 cut(s) 880, 891, 1096, 1404, 1420, 1703, 2126
CspCI CAANNNNNGTGG 3 cut(s) 2520, 2536, 2555
CviQI GTAC 7 cut(s) 880, 891, 1096, 1404, 1420, 1703, 2126
DdeI CTNAG 6 cut(s) 13, 168, 366, 537, 1449, 1628
DpnI GATC 9 cut(s) 183, 291, 678, 859, 1053, 1329, 1834, 2151, 2472
DpnII GATC 9 cut(s) 181, 289, 676, 857, 1051, 1327, 1832, 2149, 2470
EaeI YGGCCR 1 cut(s) 586
Eam1104I CTCTTC 2 cut(s) 485, 2320
EarI CTCTTC 2 cut(s) 485, 2320
EciI GGCGGA 2 cut(s) 1674, 1845
Eco130I CCWWGG 5 cut(s) 644, 883, 1196, 2277, 2346
Eco147I AGGCCT 1 cut(s) 2035
Eco24I GRGCYC 2 cut(s) 330, 920
Eco47I GGWCC 4 cut(s) 521, 791, 1200, 1752
Eco57I CTGAAG 3 cut(s) 406, 419, 2220
Eco72I CACGTG 1 cut(s) 2555
Eco81I CCTNAGG 1 cut(s) 168
Eco91I GGTNACC 1 cut(s) 992
EcoO109I RGGNCCY 2 cut(s) 916, 917
EcoO65I GGTNACC 1 cut(s) 992
EcoRII CCWGG 7 cut(s) 259, 409, 513, 1366, 1719, 1758, 2299
EcoT14I CCWWGG 5 cut(s) 644, 883, 1196, 2277, 2346
EcoT38I GRGCYC 2 cut(s) 330, 920
ErhI CCWWGG 5 cut(s) 644, 883, 1196, 2277, 2346
Esp3I CGTCTC 2 cut(s) 115, 1800
FaqI GGGAC 4 cut(s) 495, 496, 507, 1745
FauNDI CATATG 2 cut(s) 88, 826
Fnu4HI GCNGC 8 cut(s) 53, 582, 609, 980, 1155, 1856, 2059, 2495
FokI GGATG 1 cut(s) 1355
FriOI GRGCYC 2 cut(s) 330, 920
Fsp4HI GCNGC 8 cut(s) 53, 582, 609, 980, 1155, 1856, 2059, 2495
FspBI CTAG 4 cut(s) 1395, 1428, 2157, 2331
GlaI GCGC 3 cut(s) 364, 561, 2291
GluI GCNGC 8 cut(s) 53, 582, 609, 980, 1155, 1856, 2059, 2495
GsaI CCCAGC 2 cut(s) 29, 59
GsuI CTGGAG 2 cut(s) 657, 2043
HaeIII GGCC 9 cut(s) 101, 351, 588, 608, 918, 1503, 1695, 1941, 2035
HapII CCGG 3 cut(s) 636, 912, 1605
HgaI GACGC 2 cut(s) 912, 1782
HhaI GCGC 3 cut(s) 365, 562, 2292
Hin6I GCGC 3 cut(s) 363, 560, 2290
HinP1I GCGC 3 cut(s) 363, 560, 2290
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HpaI GTTAAC 1 cut(s) 868
HpaII CCGG 3 cut(s) 636, 912, 1605
HphI GGTGA 7 cut(s) 307, 329, 661, 791, 986, 1573, 2437
Hpy166II GTNNAC 9 cut(s) 73, 465, 777, 868, 1031, 1096, 1098, 1513, 1752
Hpy8I GTNNAC 9 cut(s) 73, 465, 777, 868, 1031, 1096, 1098, 1513, 1752
Hpy99I CGWCG 1 cut(s) 1643
HpyCH4III ACNGT 2 cut(s) 77, 701
HpyCH4IV ACGT 5 cut(s) 1265, 1600, 2393, 2538, 2554
HpyF10VI GCNNNNNNNGC 5 cut(s) 617, 1282, 2010, 2193, 2516
HpyF3I CTNAG 6 cut(s) 13, 168, 366, 537, 1449, 1628
HpySE526I ACGT 5 cut(s) 1265, 1600, 2393, 2538, 2554
HspAI GCGC 3 cut(s) 363, 560, 2290
KpnI GGTACC 1 cut(s) 894
KspAI GTTAAC 1 cut(s) 868
Kzo9I GATC 9 cut(s) 181, 289, 676, 857, 1051, 1327, 1832, 2149, 2470
LmnI GCTCC 8 cut(s) 557, 578, 676, 955, 1003, 1369, 2361, 2524
Lsp1109I GCAGC 4 cut(s) 39, 991, 1141, 1867
LweI GCATC 4 cut(s) 423, 658, 937, 1290
MabI ACCWGGT 1 cut(s) 1758
MaeI CTAG 4 cut(s) 1395, 1428, 2157, 2331
MaeII ACGT 5 cut(s) 1265, 1600, 2393, 2538, 2554
MaeIII GTNAC 7 cut(s) 82, 695, 992, 1266, 1390, 2294, 2443
MalI GATC 9 cut(s) 183, 291, 678, 859, 1053, 1329, 1834, 2151, 2472
MbiI CCGCTC 1 cut(s) 1364
MboI GATC 9 cut(s) 181, 289, 676, 857, 1051, 1327, 1832, 2149, 2470
MboII GAAGA 8 cut(s) 191, 502, 674, 677, 1337, 2273, 2307, 2425
MfeI CAATTG 1 cut(s) 2048
MflI RGATCY 6 cut(s) 181, 676, 1051, 1327, 2149, 2470
MhlI GDGCHC 4 cut(s) 330, 920, 1515, 2521
MlsI TGGCCA 1 cut(s) 588
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 2 cut(s) 518, 1460
MmeI TCCRAC 5 cut(s) 609, 1234, 1621, 1744, 2478
Mox20I TGGCCA 1 cut(s) 588
MscI TGGCCA 1 cut(s) 588
MslI CAYNNNNRTG 1 cut(s) 638
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 4 cut(s) 67, 584, 1049, 1154
MspI CCGG 3 cut(s) 636, 912, 1605
MspR9I CCNGG 8 cut(s) 261, 411, 515, 913, 1368, 1721, 1760, 2301
MunI CAATTG 1 cut(s) 2048
MvaI CCWGG 7 cut(s) 261, 411, 515, 1368, 1721, 1760, 2301
MwoI GCNNNNNNNGC 5 cut(s) 617, 1282, 2010, 2193, 2516
NciI CCSGG 1 cut(s) 913
NcoI CCATGG 2 cut(s) 644, 883
NdeI CATATG 2 cut(s) 88, 826
NdeII GATC 9 cut(s) 181, 289, 676, 857, 1051, 1327, 1832, 2149, 2470
NheI GCTAGC 1 cut(s) 2330
NmeAIII GCCGAG 1 cut(s) 1671
NmuCI GTSAC 5 cut(s) 82, 992, 1266, 1390, 2443
NspI RCATGY 2 cut(s) 880, 1180
OliI CACNNNNGTG 1 cut(s) 638
PasI CCCWGGG 2 cut(s) 514, 2300
PceI AGGCCT 1 cut(s) 2035
PciI ACATGT 1 cut(s) 876
PfeI GAWTC 9 cut(s) 126, 187, 713, 1106, 1142, 1442, 1572, 1686, 1786
PflMI CCANNNNNTGG 1 cut(s) 989
PkrI GCNGC 8 cut(s) 54, 583, 610, 981, 1156, 1857, 2060, 2496
PleI GAGTC 2 cut(s) 517, 1459
PmaCI CACGTG 1 cut(s) 2555
PmlI CACGTG 1 cut(s) 2555
PpsI GAGTC 2 cut(s) 517, 1459
Ppu21I YACGTR 2 cut(s) 1601, 2555
PscI ACATGT 1 cut(s) 876
PshBI ATTAAT 4 cut(s) 111, 1524, 1838, 1953
Psp6I CCWGG 7 cut(s) 259, 409, 513, 1366, 1719, 1758, 2299
PspCI CACGTG 1 cut(s) 2555
PspEI GGTNACC 1 cut(s) 992
PspFI CCCAGC 2 cut(s) 25, 55
PspGI CCWGG 7 cut(s) 259, 409, 513, 1366, 1719, 1758, 2299
PspOMI GGGCCC 1 cut(s) 916
PspPI GGNCC 9 cut(s) 350, 521, 606, 791, 916, 917, 1200, 1694, 1752
PstNI CAGNNNCTG 1 cut(s) 1544
PsuI RGATCY 6 cut(s) 181, 676, 1051, 1327, 2149, 2470
PvuII CAGCTG 2 cut(s) 67, 1154
RsaI GTAC 7 cut(s) 881, 892, 1097, 1405, 1421, 1704, 2127
RsaNI GTAC 7 cut(s) 880, 891, 1096, 1404, 1420, 1703, 2126
RseI CAYNNNNRTG 1 cut(s) 638
SatI GCNGC 8 cut(s) 53, 582, 609, 980, 1155, 1856, 2059, 2495
Sau3AI GATC 9 cut(s) 181, 289, 676, 857, 1051, 1327, 1832, 2149, 2470
Sau96I GGNCC 9 cut(s) 350, 521, 606, 791, 916, 917, 1200, 1694, 1752
ScaI AGTACT 1 cut(s) 1405
SchI GAGTC 2 cut(s) 518, 1460
ScrFI CCNGG 8 cut(s) 261, 411, 515, 913, 1368, 1721, 1760, 2301
SduI GDGCHC 4 cut(s) 330, 920, 1515, 2521
SexAI ACCWGGT 1 cut(s) 1758
SfaNI GCATC 4 cut(s) 423, 658, 937, 1290
SinI GGWCC 4 cut(s) 521, 791, 1200, 1752
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 2 cut(s) 305, 998
SmoI CTYRAG 2 cut(s) 305, 998
SseBI AGGCCT 1 cut(s) 2035
SspI AATATT 1 cut(s) 1294
SspMI CTAG 4 cut(s) 1395, 1428, 2157, 2331
StuI AGGCCT 1 cut(s) 2035
StyD4I CCNGG 8 cut(s) 259, 409, 513, 911, 1366, 1719, 1758, 2299
StyI CCWWGG 5 cut(s) 644, 883, 1196, 2277, 2346
TaaI ACNGT 2 cut(s) 77, 701
TaiI ACGT 5 cut(s) 1268, 1603, 2396, 2541, 2557
TaqI TCGA 2 cut(s) 1818, 2241
TaqII GACCGA 1 cut(s) 2082
TatI WGTACW 3 cut(s) 1095, 1403, 1702
TauI GCSGC 4 cut(s) 584, 611, 2061, 2497
TfiI GAWTC 9 cut(s) 126, 187, 713, 1106, 1142, 1442, 1572, 1686, 1786
TscAI CASTG 6 cut(s) 80, 687, 1074, 1249, 1629, 2194
TseFI GTSAC 5 cut(s) 82, 992, 1266, 1390, 2443
TseI GCWGC 4 cut(s) 52, 979, 1154, 1855
Tsp45I GTSAC 5 cut(s) 82, 992, 1266, 1390, 2443
TspGWI ACGGA 1 cut(s) 794
TspRI CASTG 6 cut(s) 80, 687, 1074, 1249, 1629, 2194
Van91I CCANNNNNTGG 1 cut(s) 989
VneI GTGCAC 1 cut(s) 1511
VpaK11BI GGWCC 4 cut(s) 521, 791, 1200, 1752
VspI ATTAAT 4 cut(s) 111, 1524, 1838, 1953
XapI RAATTY 2 cut(s) 1075, 2324
XbaI TCTAGA 1 cut(s) 2156
XceI RCATGY 2 cut(s) 880, 1180
XspI CTAG 4 cut(s) 1395, 1428, 2157, 2331
ZrmI AGTACT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.