Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001965

Physical Location & Seq

Genomic Coordinates Forward (+)

22908790 .. 22940939

Gene Structure 32150 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G012580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGGTCTTCTCGTCTCCATGTCTTCAGCTGACAAAGTGCAATGTTACCTATGATAGGGAGGCTCTTCTCGTCAATGGACAGAGGAGAATTCTCTTTTCTGGTTCCATACACTATCCTAGGAGTACTCCTGAGATGTGGGAAGATCTTATACTGAAGGCAAAAGATGGTGGCTTGGATGTCATAGACACCTATGTGTTTTGGAATCTTCATGAGCCTTCTCCGGGCAATGTACTGTCTGGTTCAAACAGCAGGGCTCTCTATGTGCATCTTCGCATTGGACCTTATGTATGTGCAGAATGGAATTTTGGGGGGTTTCCAGTTTGGCTCAAGTATGTTCCAAGCATTAGCTTCAGAACAAATAATGAGCCTTTCAAGGCAGCAATGCAGACGTTTACTCAGAAAATTGTCCAATTGATGAAGGATGAGGAATTATTTGAGTCTCAAGGTGGTCCCATTTGTTGTGGGAGAATAGAATTGTGTATTGAGAATGAGTACGAGCACTTGAGCAAGGCTTTTGGGGAAGCCGGTCATGCATACATGACATGGGCTGCAGAGATGGCTGTTGGAATGGATACTGGGGTCCCATGGGTCATGTGCAAGGAAGATGATGCCCCAGACCCTGTGATAAACACTTGCAATGGGTTTTATTGTGACAATTTTTCTCCAAACAAACCATACAAGCCCACGATCTGGACTGAGGCTTGGACTGGCTGCGATCGGCCTGTTGAAGATTTGGCATTTGCAGTTGCTCGATTTGTTCAGAACGGAGGCTCCTTCATGAACTATTACATGTACCATAGAGGAACTAACTTTGAAAGAACTGCTGGAGGCCCTTTTATTATTACCAGCTATGACTACGATGCCCCCATTGATGAATATGGCTTGATCAGGCAACCCAAGTATGGTCACTTAATGGAACTCCATAAAGCTGTTAAGTTATGTGAGAAAGCTCTGCTAAATGCTGATCCGACTGTTACATCCTTAGGGAGATATCAACAGTCACATGTATTCTCTTCAAAGCCAGGAGGTTGTGCAGCATTTCTCTCAAACTACAATGCAAACTCAGCTGCAACGGTGACTTTCAATGACCGAAACTATGAGCTCCCTGCTTGGTCTATCAGCATCCTTCCAGACTGCGAAAACGTTGTATTCAACACTGCCAAAGTGGGAGTTCAGACATCCCAAAAACAATTTTTTCCCACTAACTCTGAGTTGTGGTCATGGGAAACCTTCAGTGAAGACATTGCTTCATGGACTGGTGATACAACTCTCACAGTCATTGGTTTCTTAGATCAGTTGAACATCACTAGAGACTCCAGTGATTATCTATGGTACACCACCAGTGTCGACATAAGTCCATCTGAATCATTTTTGCGTGGAGGTAAGCATCCAATTCTCACCGTGCAGTCTGTTGGGAACGCTATGCATGTCTTTATCAATGACCAGCTCTCAGACCAGAGATTCACTTTTACAGGAGATGTAAACATGCATGTTGGTGTGAATAAAATTTCACTTCTAAGTGTGGCTAACAATGGACCTCACTCTGAGACATGGAACTTGGGAGTACTAGGACCAGCTGTTCTACAAGGATTGGATCAAGGAAGCAGAGATTTATCAGGGCAGTCATGGTCATACAAGGTTGGGCTAAAAGGAGAATCCATGAATCTCGGTTCTCCAACTTCCACCTCTGCAGTGAATTGGACGAGGGTGTTTATAGGGACACAGAATCAACAACCACTGACTTGGTATAAGATCAATGGGCAGAGTATTGGAAGATACTGGACTATTCATGCTAATGGTAACTGCAGCGCATGCAGTTATTCTGGTACATTCAGGATTAACAAGTGTCAATTTGGTTGTCTCCAACCAACTCAACAATGGTATCATGTTCCTCGGTCTTGGCTCAAGCCATCTCAAAATCTGTTGGTTGTTTTTGAGGAGCTTGATGGCGATGTTTCAAAGATTGATCTTGTGAAAACATCCATGAGGACCATCTGTGTTGAGATTTGTGAAAGTCACCCACTCGTTAGGGAATTGGACACCAAGAGCCAAGGCAAATTGAAAGAGCAAAAGAAGCCTAAACTTAGCCTACAGTGTAAAAATGGGCACTCTATTTCTGCTATTAAATTTTCAAGCTTTGGTACTCCATCCGGAAGCTGTGGGAACTATAAGCATTGTTCTTACTTTTTCCTACTATTTTTATATCTACAGAAGTGCGTAGGTAAGCAGAAGTGTTCAGTGAACGTATCGAATGCAAACTTTGGCATGGACCCTTGCCCAGGCACAATGAAAAAATTATCTGTGGAAGCTATTTGTGCCCTTATAACTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0009505 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

777

Amino Acids

86.82

Weight (kDa)

6.33

Isoelectric Point (pI)

35.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	22 - 310	5.2e-90	Glycosyl hydrolases family 35
GHD	PF17834	333 - 389	9.2e-25	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	585 - 640	2e-11	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	697 - 772	9.3e-16	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36360 AT4G36360
fragaria_vesca	FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_2g09820 FvH4_2g09820
malus_domestica	MD02G1137500.v1.1 MD10G1067800.v1.1 MD15G1251100.v1.1
prunus_persica	Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.8G089500_v2.0.a1 Prupe.8G089500_v2.0.a1
pyrus_communis	pycom02g10880 pycom10g05360 pycom15g22140
rosa_chinensis	RchiOBHm_Chr2g0100171 RchiOBHm_Chr6g0266171
rosa_laevigata	RLG00000014106 RLG00000014109 RLG00000016943
rosa_multiflora	Rmu_sc0002547.1_g000001
rosa_roxburghii	Rroxscaffold_174G00435340 Rroxscaffold_2G00142500 Rroxscaffold_7G00201780 Rroxscaffold_7G00201840
rosa_rugosa	Rorug02G0086200 Rorug06G0024500 Rorug06G0024900 Rorug06G0025000
rosa_samantha	Rh2AG135300 Rh2CG140500 Rh2DG140700 Rh6BG145900 Rh6CG142300 Rh6DG130400
rosa_wichuraiana	Rw2G010560 Rw6G012540 Rw6G012580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2324
AccB7I	CCANNNNNTGG	1 cut(s)	690
AccI	GTMKAC	1 cut(s)	1347
AccIII	TCCGGA	1 cut(s)	2150
AclI	AACGTT	1 cut(s)	1143
AclWI	GGATC	2 cut(s)	959, 1602
AcsI	RAATTY	4 cut(s)	87, 301, 1506, 2126
AcuI	CTGAAG	4 cut(s)	8, 173, 334, 1216
AfaI	GTAC	8 cut(s)	124, 231, 494, 794, 1334, 1566, 1828, 2143
AfiI	CCNNNNNNNGG	5 cut(s)	54, 221, 690, 902, 2279
AflIII	ACRYGT	2 cut(s)	789, 1003
AjnI	CCWGG	2 cut(s)	1021, 2278
AjuI	GAANNNNNNNTTGG	2 cut(s)	288, 320
AleI	CACNNNNGTG	1 cut(s)	191
AloI	GAACNNNNNNTCC	4 cut(s)	755, 787, 1563, 1595
Alw21I	GWGCWC	2 cut(s)	501, 1104
Alw26I	GTCTC	5 cut(s)	18, 444, 1305, 1541, 1865
AlwI	GGATC	2 cut(s)	959, 1602
AlwNI	CAGNNNCTG	1 cut(s)	620
Aor13HI	TCCGGA	1 cut(s)	2150
AoxI	GGCC	2 cut(s)	719, 829
ApeKI	GCWGC	6 cut(s)	377, 548, 711, 1034, 1067, 1806
ApoI	RAATTY	4 cut(s)	87, 301, 1506, 2126
AspA2I	CCTAGG	1 cut(s)	116
AspLEI	GCGC	1 cut(s)	1811
AspS9I	GGNCC	8 cut(s)	278, 449, 580, 830, 1535, 1571, 1989, 2269
AsuC2I	CCSGG	1 cut(s)	222
AsuHPI	GGTGA	4 cut(s)	1087, 1271, 1390, 2009
AvaII	GGWCC	7 cut(s)	278, 449, 580, 1535, 1571, 1989, 2269
AvrII	CCTAGG	1 cut(s)	116
AxyI	CCTNAGG	1 cut(s)	982
BaeGI	GKGCMC	2 cut(s)	2109, 2320
BaeI	ACNNNNGTAYC	4 cut(s)	564, 597, 1865, 1898
BanII	GRGCYC	2 cut(s)	256, 1104
BarI	GAAGNNNNNNTAC	2 cut(s)	132, 164
BbsI	GAAGAC	2 cut(s)	14, 1245
Bbv12I	GWGCWC	2 cut(s)	501, 1104
BbvI	GCAGC	6 cut(s)	389, 535, 698, 1046, 1054, 1818
BccI	CCATC	7 cut(s)	158, 550, 1366, 1918, 1940, 2000, 2155
BciT130I	CCWGG	2 cut(s)	1023, 2280
BciVI	GTATCC	1 cut(s)	565
BclI	TGATCA	1 cut(s)	885
BcnI	CCSGG	1 cut(s)	222
BcoDI	GTCTC	5 cut(s)	18, 444, 1305, 1541, 1865
BfaI	CTAG	3 cut(s)	117, 1308, 1568
BfmI	CTRYAG	5 cut(s)	549, 1689, 1804, 2090, 2207
BfuI	GTATCC	1 cut(s)	565
BglII	AGATCT	1 cut(s)	142
BisI	GCNGC	6 cut(s)	378, 549, 712, 1035, 1068, 1807
BlnI	CCTAGG	1 cut(s)	116
BlsI	GCNGC	6 cut(s)	379, 550, 713, 1036, 1069, 1808
BmcAI	AGTACT	2 cut(s)	124, 1566
Bme1390I	CCNGG	3 cut(s)	222, 1023, 2280
Bme18I	GGWCC	7 cut(s)	278, 449, 580, 1535, 1571, 1989, 2269
BmgT120I	GGNCC	8 cut(s)	278, 449, 580, 830, 1535, 1571, 1989, 2269
BmiI	GGNNCC	6 cut(s)	103, 451, 581, 582, 772, 2271
BmrFI	CCNGG	3 cut(s)	222, 1023, 2280
BmrI	ACTGGG	1 cut(s)	585
BmsI	GCATC	5 cut(s)	274, 598, 850, 1131, 1396
BmuI	ACTGGG	1 cut(s)	585
BoxI	GACNNNNGTC	1 cut(s)	1353
BpiI	GAAGAC	2 cut(s)	14, 1245
BpmI	CTGGAG	2 cut(s)	846, 1300
BpuEI	CTTGAG	4 cut(s)	311, 426, 523, 1889
BpuMI	CCSGG	1 cut(s)	222
BsaJI	CCNNGG	5 cut(s)	116, 584, 1892, 2050, 2278
BsaWI	WCCGGW	1 cut(s)	2150
BsaXI	ACNNNNNCTCC	2 cut(s)	755, 785
Bsc4I	CCNNNNNNNGG	5 cut(s)	54, 221, 690, 902, 2279
Bse118I	RCCGGY	1 cut(s)	524
Bse1I	ACTGG	7 cut(s)	317, 580, 712, 1261, 1317, 1341, 1784
Bse21I	CCTNAGG	1 cut(s)	982
Bse3DI	GCAATG	5 cut(s)	46, 232, 387, 643, 1242
BseAI	TCCGGA	1 cut(s)	2150
BseBI	CCWGG	2 cut(s)	1023, 2280
BseDI	CCNNGG	5 cut(s)	116, 584, 1892, 2050, 2278
BseGI	GGATG	8 cut(s)	181, 427, 977, 1122, 1178, 1387, 1979, 2147
BseLI	CCNNNNNNNGG	5 cut(s)	54, 221, 690, 902, 2279
BseMI	GCAATG	5 cut(s)	46, 232, 387, 643, 1242
BseMII	CTCAG	7 cut(s)	120, 410, 687, 1077, 1200, 1464, 1536
BseNI	ACTGG	7 cut(s)	317, 580, 712, 1261, 1317, 1341, 1784
BseRI	GAGGAG	2 cut(s)	97, 1952
BseSI	GKGCMC	2 cut(s)	2109, 2320
BseXI	GCAGC	6 cut(s)	389, 535, 698, 1046, 1054, 1818
BsgI	GTGCAG	3 cut(s)	312, 1053, 1424
Bsh1285I	CGRYCG	1 cut(s)	718
BshFI	GGCC	2 cut(s)	721, 831
BsiEI	CGRYCG	1 cut(s)	718
BsiHKAI	GWGCWC	2 cut(s)	501, 1104
BsiSI	CCGG	3 cut(s)	221, 525, 2151
BslFI	GGGAC	3 cut(s)	435, 566, 1732
BslI	CCNNNNNNNGG	5 cut(s)	54, 221, 690, 902, 2279
BsmAI	GTCTC	5 cut(s)	18, 444, 1305, 1541, 1865
BsmBI	CGTCTC	1 cut(s)	18
BsmFI	GGGAC	3 cut(s)	435, 566, 1732
BsmI	GAATGC	1 cut(s)	2257
BsnI	GGCC	2 cut(s)	721, 831
Bsp1286I	GDGCHC	5 cut(s)	256, 501, 1104, 2109, 2320
Bsp13I	TCCGGA	1 cut(s)	2150
Bsp143I	GATC	9 cut(s)	142, 687, 715, 885, 964, 1291, 1594, 1752, 1966
Bsp19I	CCATGG	1 cut(s)	584
BspANI	GGCC	2 cut(s)	721, 831
BspCNI	CTCAG	7 cut(s)	121, 409, 688, 1076, 1201, 1463, 1537
BspEI	TCCGGA	1 cut(s)	2150
BspHI	TCATGA	2 cut(s)	208, 777
BspLI	GGNNCC	6 cut(s)	103, 451, 581, 582, 772, 2271
BspMAI	CTGCAG	3 cut(s)	553, 1693, 1808
BspPI	GGATC	2 cut(s)	959, 1602
BspQI	GCTCTTC	1 cut(s)	69
BsrDI	GCAATG	5 cut(s)	46, 232, 387, 643, 1242
BsrFI	RCCGGY	1 cut(s)	524
BsrI	ACTGG	7 cut(s)	317, 580, 712, 1261, 1317, 1341, 1784
BssAI	RCCGGY	1 cut(s)	524
BssECI	CCNNGG	5 cut(s)	116, 584, 1892, 2050, 2278
BssMI	GATC	9 cut(s)	142, 687, 715, 885, 964, 1291, 1594, 1752, 1966
BssT1I	CCWWGG	3 cut(s)	116, 584, 2050
Bst2UI	CCWGG	2 cut(s)	1023, 2280
Bst4CI	ACNGT	7 cut(s)	234, 973, 999, 1075, 1276, 1402, 2094
Bst6I	CTCTTC	2 cut(s)	69, 1018
BstAPI	GCANNNNNTGC	1 cut(s)	1812
BstC8I	GCNNGC	1 cut(s)	1813
BstDSI	CCRYGG	1 cut(s)	584
BstENI	CCTNNNNNAGG	2 cut(s)	52, 2277
BstF5I	GGATG	8 cut(s)	181, 427, 977, 1122, 1178, 1387, 1979, 2147
BstHHI	GCGC	1 cut(s)	1811
BstKTI	GATC	9 cut(s)	145, 690, 718, 888, 967, 1294, 1597, 1755, 1969
BstMAI	GTCTC	5 cut(s)	18, 444, 1305, 1541, 1865
BstMBI	GATC	9 cut(s)	142, 687, 715, 885, 964, 1291, 1594, 1752, 1966
BstMCI	CGRYCG	1 cut(s)	718
BstMWI	GCNNNNNNNGC	6 cut(s)	530, 557, 1064, 1812, 2074, 2315
BstNI	CCWGG	2 cut(s)	1023, 2280
BstNSI	RCATGY	6 cut(s)	793, 1007, 1430, 1489, 1493, 1815
BstPAI	GACNNNNGTC	1 cut(s)	1353
BstSCI	CCNGG	3 cut(s)	220, 1021, 2278
BstSFI	CTRYAG	5 cut(s)	549, 1689, 1804, 2090, 2207
BstSLI	GKGCMC	2 cut(s)	2109, 2320
BstV1I	GCAGC	6 cut(s)	389, 535, 698, 1046, 1054, 1818
BstV2I	GAAGAC	2 cut(s)	14, 1245
BstX2I	RGATCY	1 cut(s)	142
BstXI	CCANNNNNNTGG	1 cut(s)	1743
BstYI	RGATCY	1 cut(s)	142
Bsu36I	CCTNAGG	1 cut(s)	982
BsuI	GTATCC	1 cut(s)	565
BsuRI	GGCC	2 cut(s)	721, 831
BtgI	CCRYGG	1 cut(s)	584
BtgZI	GCGATG	1 cut(s)	1965
BtsCI	GGATG	8 cut(s)	181, 427, 977, 1122, 1178, 1387, 1979, 2147
BtsI	GCAGTG	2 cut(s)	1155, 1698
BtsIMutI	CAGTG	8 cut(s)	1155, 1240, 1324, 1348, 1698, 1736, 2099, 2244
Cac8I	GCNNGC	1 cut(s)	1813
CaiI	CAGNNNCTG	1 cut(s)	620
CciI	TCATGA	2 cut(s)	208, 777
CfoI	GCGC	1 cut(s)	1811
Cfr10I	RCCGGY	1 cut(s)	524
Cfr13I	GGNCC	8 cut(s)	278, 449, 580, 830, 1535, 1571, 1989, 2269
Csp6I	GTAC	8 cut(s)	123, 230, 493, 793, 1333, 1565, 1827, 2142
CviQI	GTAC	8 cut(s)	123, 230, 493, 793, 1333, 1565, 1827, 2142
DpnI	GATC	9 cut(s)	144, 689, 717, 887, 966, 1293, 1596, 1754, 1968
DpnII	GATC	9 cut(s)	142, 687, 715, 885, 964, 1291, 1594, 1752, 1966
Eam1104I	CTCTTC	2 cut(s)	69, 1018
EarI	CTCTTC	2 cut(s)	69, 1018
Ecl136II	GAGCTC	1 cut(s)	1102
Eco130I	CCWWGG	3 cut(s)	116, 584, 2050
Eco24I	GRGCYC	2 cut(s)	256, 1104
Eco32I	GATATC	1 cut(s)	992
Eco47I	GGWCC	7 cut(s)	278, 449, 580, 1535, 1571, 1989, 2269
Eco53kI	GAGCTC	1 cut(s)	1102
Eco57I	CTGAAG	4 cut(s)	8, 173, 334, 1216
Eco81I	CCTNAGG	1 cut(s)	982
EcoICRI	GAGCTC	1 cut(s)	1102
EcoNI	CCTNNNNNAGG	2 cut(s)	52, 2277
EcoO109I	RGGNCCY	2 cut(s)	580, 830
EcoRI	GAATTC	1 cut(s)	87
EcoRII	CCWGG	2 cut(s)	1021, 2278
EcoRV	GATATC	1 cut(s)	992
EcoT14I	CCWWGG	3 cut(s)	116, 584, 2050
EcoT22I	ATGCAT	3 cut(s)	535, 1428, 1491
EcoT38I	GRGCYC	2 cut(s)	256, 1104
ErhI	CCWWGG	3 cut(s)	116, 584, 2050
Esp3I	CGTCTC	1 cut(s)	18
FaqI	GGGAC	3 cut(s)	435, 566, 1732
FbaI	TGATCA	1 cut(s)	885
FblI	GTMKAC	1 cut(s)	1347
Fnu4HI	GCNGC	6 cut(s)	378, 549, 712, 1035, 1068, 1807
FokI	GGATG	8 cut(s)	188, 434, 964, 1109, 1165, 1374, 1966, 2134
FriOI	GRGCYC	2 cut(s)	256, 1104
Fsp4HI	GCNGC	6 cut(s)	378, 549, 712, 1035, 1068, 1807
FspBI	CTAG	3 cut(s)	117, 1308, 1568
GlaI	GCGC	1 cut(s)	1810
GluI	GCNGC	6 cut(s)	378, 549, 712, 1035, 1068, 1807
GsuI	CTGGAG	2 cut(s)	846, 1300
HaeIII	GGCC	2 cut(s)	721, 831
HapII	CCGG	3 cut(s)	221, 525, 2151
HhaI	GCGC	1 cut(s)	1811
Hin6I	GCGC	1 cut(s)	1809
HinP1I	GCGC	1 cut(s)	1809
HincII	GTYRAC	1 cut(s)	1348
HindII	GTYRAC	1 cut(s)	1348
HindIII	AAGCTT	1 cut(s)	2134
HinfI	GANTC	8 cut(s)	202, 437, 1313, 1364, 1461, 1655, 1663, 1726
HpaII	CCGG	3 cut(s)	221, 525, 2151
HphI	GGTGA	4 cut(s)	1087, 1271, 1390, 2009
Hpy166II	GTNNAC	5 cut(s)	393, 1335, 1348, 1483, 2242
Hpy188I	TCNGA	9 cut(s)	353, 399, 762, 969, 1176, 1210, 1363, 1453, 1546
Hpy188III	TCNNGA	7 cut(s)	128, 209, 691, 778, 1130, 1834, 2151
Hpy8I	GTNNAC	5 cut(s)	393, 1335, 1348, 1483, 2242
HpyAV	CCTTC	6 cut(s)	148, 225, 412, 784, 1136, 1240
HpyCH4III	ACNGT	7 cut(s)	234, 973, 999, 1075, 1276, 1402, 2094
HpyCH4IV	ACGT	3 cut(s)	389, 1143, 2244
HpyF10VI	GCNNNNNNNGC	6 cut(s)	530, 557, 1064, 1812, 2074, 2315
HpySE526I	ACGT	3 cut(s)	389, 1143, 2244
HspAI	GCGC	1 cut(s)	1809
KflI	GGGWCCC	1 cut(s)	580
Kpn2I	TCCGGA	1 cut(s)	2150
Ksp22I	TGATCA	1 cut(s)	885
Kzo9I	GATC	9 cut(s)	142, 687, 715, 885, 964, 1291, 1594, 1752, 1966
LguI	GCTCTTC	1 cut(s)	69
LmnI	GCTCC	3 cut(s)	776, 1107, 1939
Lsp1109I	GCAGC	6 cut(s)	389, 535, 698, 1046, 1054, 1818
LweI	GCATC	5 cut(s)	274, 598, 850, 1131, 1396
MaeI	CTAG	3 cut(s)	117, 1308, 1568
MaeII	ACGT	3 cut(s)	389, 1143, 2244
MaeIII	GTNAC	8 cut(s)	43, 650, 905, 973, 999, 1075, 1799, 2015
MalI	GATC	9 cut(s)	144, 689, 717, 887, 966, 1293, 1596, 1754, 1968
MboI	GATC	9 cut(s)	142, 687, 715, 885, 964, 1291, 1594, 1752, 1966
MfeI	CAATTG	1 cut(s)	410
MflI	RGATCY	1 cut(s)	142
MhlI	GDGCHC	5 cut(s)	256, 501, 1104, 2109, 2320
MlyI	GAGTC	2 cut(s)	446, 1307
MmeI	TCCRAC	4 cut(s)	544, 992, 1700, 1888
Mph1103I	ATGCAT	3 cut(s)	535, 1428, 1491
MroI	TCCGGA	1 cut(s)	2150
MseI	TTAA	4 cut(s)	911, 933, 1839, 2124
MslI	CAYNNNNRTG	3 cut(s)	191, 1494, 1794
MspA1I	CMGCKG	3 cut(s)	28, 1067, 1577
MspI	CCGG	3 cut(s)	221, 525, 2151
MspR9I	CCNGG	3 cut(s)	222, 1023, 2280
MunI	CAATTG	1 cut(s)	410
Mva1269I	GAATGC	1 cut(s)	2257
MvaI	CCWGG	2 cut(s)	1023, 2280
MwoI	GCNNNNNNNGC	6 cut(s)	530, 557, 1064, 1812, 2074, 2315
NciI	CCSGG	1 cut(s)	222
NcoI	CCATGG	1 cut(s)	584
NdeII	GATC	9 cut(s)	142, 687, 715, 885, 964, 1291, 1594, 1752, 1966
NlaIV	GGNNCC	6 cut(s)	103, 451, 581, 582, 772, 2271
NmuCI	GTSAC	5 cut(s)	650, 905, 999, 1075, 2015
NsiI	ATGCAT	3 cut(s)	535, 1428, 1491
NspI	RCATGY	6 cut(s)	793, 1007, 1430, 1489, 1493, 1815
OliI	CACNNNNGTG	1 cut(s)	191
PaeI	GCATGC	1 cut(s)	1815
PagI	TCATGA	2 cut(s)	208, 777
PciI	ACATGT	2 cut(s)	789, 1003
PciSI	GCTCTTC	1 cut(s)	69
PctI	GAATGC	1 cut(s)	2257
PfeI	GAWTC	6 cut(s)	202, 1364, 1461, 1655, 1663, 1726
PflMI	CCANNNNNTGG	1 cut(s)	690
PkrI	GCNGC	6 cut(s)	379, 550, 713, 1036, 1069, 1808
Ple19I	CGATCG	1 cut(s)	718
PleI	GAGTC	2 cut(s)	445, 1307
PpsI	GAGTC	2 cut(s)	445, 1307
PpuMI	RGGWCCY	1 cut(s)	580
PscI	ACATGT	2 cut(s)	789, 1003
PshAI	GACNNNNGTC	1 cut(s)	1353
PsiI	TTATAA	1 cut(s)	2324
Psp124BI	GAGCTC	1 cut(s)	1104
Psp1406I	AACGTT	1 cut(s)	1143
Psp5II	RGGWCCY	1 cut(s)	580
Psp6I	CCWGG	2 cut(s)	1021, 2278
PspGI	CCWGG	2 cut(s)	1021, 2278
PspN4I	GGNNCC	6 cut(s)	103, 451, 581, 582, 772, 2271
PspPI	GGNCC	8 cut(s)	278, 449, 580, 830, 1535, 1571, 1989, 2269
PspPPI	RGGWCCY	1 cut(s)	580
PstI	CTGCAG	3 cut(s)	553, 1693, 1808
PstNI	CAGNNNCTG	1 cut(s)	620
PsuI	RGATCY	1 cut(s)	142
PvuI	CGATCG	1 cut(s)	718
PvuII	CAGCTG	3 cut(s)	28, 1067, 1577
RsaI	GTAC	8 cut(s)	124, 231, 494, 794, 1334, 1566, 1828, 2143
RsaNI	GTAC	8 cut(s)	123, 230, 493, 793, 1333, 1565, 1827, 2142
RseI	CAYNNNNRTG	3 cut(s)	191, 1494, 1794
SacI	GAGCTC	1 cut(s)	1104
SalI	GTCGAC	1 cut(s)	1346
SapI	GCTCTTC	1 cut(s)	69
SaqAI	TTAA	4 cut(s)	911, 933, 1839, 2124
SatI	GCNGC	6 cut(s)	378, 549, 712, 1035, 1068, 1807
Sau3AI	GATC	9 cut(s)	142, 687, 715, 885, 964, 1291, 1594, 1752, 1966
Sau96I	GGNCC	8 cut(s)	278, 449, 580, 830, 1535, 1571, 1989, 2269
ScaI	AGTACT	2 cut(s)	124, 1566
SchI	GAGTC	2 cut(s)	446, 1307
ScrFI	CCNGG	3 cut(s)	222, 1023, 2280
SduI	GDGCHC	5 cut(s)	256, 501, 1104, 2109, 2320
SfaNI	GCATC	5 cut(s)	274, 598, 850, 1131, 1396
SfcI	CTRYAG	5 cut(s)	549, 1689, 1804, 2090, 2207
SinI	GGWCC	7 cut(s)	278, 449, 580, 1535, 1571, 1989, 2269
SmiMI	CAYNNNNRTG	3 cut(s)	191, 1494, 1794
SmlI	CTYRAG	4 cut(s)	326, 441, 502, 1904
SmoI	CTYRAG	4 cut(s)	326, 441, 502, 1904
SphI	GCATGC	1 cut(s)	1815
SspMI	CTAG	3 cut(s)	117, 1308, 1568
SstI	GAGCTC	1 cut(s)	1104
StyD4I	CCNGG	3 cut(s)	220, 1021, 2278
StyI	CCWWGG	3 cut(s)	116, 584, 2050
TaaI	ACNGT	7 cut(s)	234, 973, 999, 1075, 1276, 1402, 2094
TaiI	ACGT	3 cut(s)	392, 1146, 2247
TaqI	TCGA	3 cut(s)	751, 1347, 2249
TaqII	GACCGA	2 cut(s)	1104, 1884
TatI	WGTACW	3 cut(s)	122, 229, 1564
TfiI	GAWTC	6 cut(s)	202, 1364, 1461, 1655, 1663, 1726
Tru1I	TTAA	4 cut(s)	911, 933, 1839, 2124
Tru9I	TTAA	4 cut(s)	911, 933, 1839, 2124
TscAI	CASTG	8 cut(s)	1162, 1240, 1324, 1348, 1698, 1743, 2099, 2244
TseFI	GTSAC	5 cut(s)	650, 905, 999, 1075, 2015
TseI	GCWGC	6 cut(s)	377, 548, 711, 1034, 1067, 1806
Tsp45I	GTSAC	5 cut(s)	650, 905, 999, 1075, 2015
TspDTI	ATGAA	9 cut(s)	197, 431, 766, 794, 888, 1239, 1676, 1778, 2303
TspGWI	ACGGA	1 cut(s)	780
TspRI	CASTG	8 cut(s)	1162, 1240, 1324, 1348, 1698, 1743, 2099, 2244
Van91I	CCANNNNNTGG	1 cut(s)	690
VpaK11BI	GGWCC	7 cut(s)	278, 449, 580, 1535, 1571, 1989, 2269
XagI	CCTNNNNNAGG	2 cut(s)	52, 2277
XapI	RAATTY	4 cut(s)	87, 301, 1506, 2126
XceI	RCATGY	6 cut(s)	793, 1007, 1430, 1489, 1493, 1815
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1875
XmaJI	CCTAGG	1 cut(s)	116
XmiI	GTMKAC	1 cut(s)	1347
XspI	CTAG	3 cut(s)	117, 1308, 1568
ZrmI	AGTACT	2 cut(s)	124, 1566
Zsp2I	ATGCAT	3 cut(s)	535, 1428, 1491

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.