Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0001965

Physical Location & Seq

Genomic Coordinates Reverse (-)

49648769 .. 49655039

Gene Structure 6271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00201840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2355 bp

ATGGGAGCTAGCTCAGCTTTGAGCTGGGTTCTGCTCTGCTTAGTTGTGTTGTCTCTACGTTTTCAGCTGATACTGTGCAATGTTAGCTATGACAGGAAGGCTCTTATCATTAATGGGCAGAGGAGAATTCTCTTTTCTGGGTCCATACACTATCCTAGAAGTACTCCTGAGATGTGGGAAGGTCTTATAGAGAAGGCCAAAGATGGTGGGTTGGATGTTATAGACACCTATGTGTTTTGGAATCTCCACGAGCCTTCTCCTGGCAATTACAATTTTGAAGGGAGAAATGATTTGGTTCGCTTCATTAAGTTAGTCCAAGAGGCACGGCTCTATGTGCATCTTCGCATTGGGCCGTACATTTGCTCAGAATGGAATTTTGGGGGATTTCCAGTTTGGCTCAAGTATGTTCCAGGCATTAGCTTCAGAACAGATAATGAGCCTTTCAAGTCAGCAATGAAAAAATTTACCAAGAAGATTGTTCAATTGATGATGGATGAAAAGCTATTTGAGTCCCAAGGTGGCCCCATCATCCTTTCTCAGATTGAGAATGAATATGAGCCAGAAGGCAAGCTTTTTGGTGCTGCTGGTCATGCATACATGACTTGGGCTGCAGAGATGGCTGTTGGAATGGGTACTGGTGTTCCATGGGTCATGTGCAAGGAAGAGGATGCCCCAGACCCAGTGATAAACACTTGCAATGGTTTTTATTGCGACTACTTTTCTCCAAACAAACCATATAAACCCACACTTTGGACTGAGGCTTGGACTGGCTGGTTTACAGAATTTGGTGGCCCAATTTACAAGCGACCTGTTGAAGATTTGGCGTTTGGAGTTGCCCGATTCATTCAGAATGGAGGATCCTTCATAAATTATTACATGTACCATGGAGGAACAAACTTTGGAAGAACTGCAGGAGGTCCATTTATTACTACCAGTTATGACTATGATGCTCCCATTGACGAATATGGCTTGATCAGGCAACCTAAATATGGCCATTTAAAGGAACTCCATAAGGCTGTTAAGTTATGTGAGCAAGCTCTGCTAAACACTGATTCTACTGTTACATCCTTAGGGACTTATGAACAGGTGGGAGTTCAAACATCCCAAAAACAGTTTTTTCCTACTAACTCTAAGTTGCCCTCATGGGAAACCTTCAGTGAAGACATCTCTTCAGTTGCTAGCAATACGAAACTCACAGTAATTGGTCTCTTAGATCAGTTGAACATCACCAGAGATTCCAGTGATTACTTATGTATCGACATTAAATCATCTGAATCATTTCTGCGTGGCGGTCAACATCCAACTCTGACCGTGCAATCAGCTGGTGATGCTATGCATGTCTTTATCAATGGGCAGCTCTCAGGATCGGCGTATGGGACTAGGAAAAACAAGAGATTCACTTTTACAGGAAATGTCATCCTGCATGCTGGATTGAATAAAATCTCACTTCTCAGTATAGCTGTTGGATTGCCGAACAATGGTCCTCACTTTGAGACACGGAATGCTGGAGTACTGGGACCAGTTGCTCTACATGGACTAGACCAAGGAAGCAGAGACTTATCATGGCAGAAATGGTCGCACGAGGTGGGCCTAAAAGGAGAAGCCATGAACCTGGGTTCTCCAAGTTCCATCTCGACAGTGGATTGGACGAGGGCGTCCTTAGCGGTGAAGAATCAACAGCCACTGACGTGGTATAAGGTCAATTTTGATGCACCTGAAGGAGATGAACCTTTGGCTTTGGACATGGGAAGTATGGGCAAGGGTCAACTATGGATCAATGGGCAAAGCATCGGAAGATACTGGACTACTTATGCTAACGGGGATTGCAGTGCGTGCAGTTATTCTGGTACCTTCAGGCCTAAGAATAAGTGCCAATATGGTTGTAACCATCCAACTCAACAATGGTACCATGTTCCTAGGTCCTGGTTAAAGCAATCTAAAAACCTATTGGTAGTTTTTGAGGAGATTGGTGGGGATGTATCAAAGATTGCTCTTGTCAAACGTTCAGTGAGCACTGTTTGTGCTGAGGTTTCTGAAAATCACCCACATAATAGGAATTGGCATACTGAGAGCCGTGGCCAACTGGAAGAGCAAAAGAAGCCTATAATGAGCCTACACTGTACGGATGGATACTCCATTTCTGCTATTAAGTTTTCAAGTTTTGGAACTCCATCTGGAAGCTGTGGGAATTTTCAGCACGGTACATGTCACGCTCCAAACTCGAAGGCTGTCATTGAGAAGAAGTGTATAGGTAAGCAGCAATGTTCATTGACCATATCAGATGCCAATTTTGGGATGGATCCATGTCCAAGCATGCTGAAAAAATTATCTGTTGAAGCTGTTTGTGCCCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0009505 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

784

Amino Acids

87.13

Weight (kDa)

7.51

Isoelectric Point (pI)

40.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 339	3.6e-122	Glycosyl hydrolases family 35
BetaGal_gal-bd	PF21467	562 - 648	4.8e-27	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	705 - 782	6.8e-21	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36360 AT4G36360
fragaria_vesca	FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_1g12350 FvH4_2g09820 FvH4_2g09820
malus_domestica	MD02G1137500.v1.1 MD10G1067800.v1.1 MD15G1251100.v1.1
prunus_persica	Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.7G163100_v2.0.a1 Prupe.8G089500_v2.0.a1 Prupe.8G089500_v2.0.a1
pyrus_communis	pycom02g10880 pycom10g05360 pycom15g22140
rosa_chinensis	RchiOBHm_Chr2g0100171 RchiOBHm_Chr6g0266171
rosa_laevigata	RLG00000014106 RLG00000014109 RLG00000016943
rosa_multiflora	Rmu_sc0002547.1_g000001
rosa_roxburghii	Rroxscaffold_174G00435340 Rroxscaffold_2G00142500 Rroxscaffold_7G00201780 Rroxscaffold_7G00201840
rosa_rugosa	Rorug02G0086200 Rorug06G0024500 Rorug06G0024900 Rorug06G0025000
rosa_samantha	Rh2AG135300 Rh2CG140500 Rh2DG140700 Rh6BG145900 Rh6CG142300 Rh6DG130400
rosa_wichuraiana	Rw2G010560 Rw6G012540 Rw6G012580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1654
Acc65I	GGTACC	2 cut(s)	1847, 1905
AccB1I	GGYRCC	2 cut(s)	1847, 1905
AccB7I	CCANNNNNTGG	1 cut(s)	750
AciI	CCGC	2 cut(s)	1290, 1664
AclI	AACGTT	1 cut(s)	2002
AclWI	GGATC	6 cut(s)	852, 865, 1372, 1781, 2294, 2307
AcoI	YGGCCR	2 cut(s)	991, 2077
AcsI	RAATTY	5 cut(s)	126, 373, 461, 782, 2188
AcuI	CTGAAG	5 cut(s)	406, 1138, 1155, 1737, 1837
AcyI	GRCGYC	1 cut(s)	1655
AdeI	CACNNNGTG	1 cut(s)	1585
AfaI	GTAC	9 cut(s)	163, 356, 634, 881, 1512, 1849, 1907, 2122, 2203
AfiI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1478, 2292
AflIII	ACRYGT	2 cut(s)	876, 2204
AgsI	TTSAA	9 cut(s)	278, 445, 482, 815, 1097, 1222, 1435, 2157, 2336
AjiI	CACGTC	1 cut(s)	1689
AjnI	CCWGG	4 cut(s)	259, 409, 1611, 1922
AjuI	GAANNNNNNNTTGG	2 cut(s)	360, 392
AleI	CACNNNNGTG	2 cut(s)	230, 1687
Alw21I	GWGCWC	1 cut(s)	2015
Alw26I	GTCTC	4 cut(s)	57, 1211, 1487, 1548
AlwI	GGATC	6 cut(s)	852, 865, 1372, 1781, 2294, 2307
AlwNI	CAGNNNCTG	1 cut(s)	1684
AoxI	GGCC	8 cut(s)	195, 350, 520, 790, 991, 1588, 1856, 2077
ApeKI	GCWGC	4 cut(s)	581, 608, 1354, 2257
ApoI	RAATTY	5 cut(s)	126, 373, 461, 782, 2188
AseI	ATTAAT	1 cut(s)	111
Asp700I	GAANNNNTTC	1 cut(s)	1278
Asp718I	GGTACC	2 cut(s)	1847, 1905
AspA2I	CCTAGG	1 cut(s)	1916
AspS9I	GGNCC	9 cut(s)	141, 350, 521, 791, 917, 1481, 1517, 1588, 1920
AsuHPI	GGTGA	4 cut(s)	1219, 1337, 1678, 2033
AsuNHI	GCTAGC	2 cut(s)	8, 1178
AvaII	GGWCC	5 cut(s)	141, 917, 1481, 1517, 1920
AvrII	CCTAGG	1 cut(s)	1916
AxyI	CCTNAGG	1 cut(s)	1069
BaeGI	GKGCMC	1 cut(s)	2350
BaeI	ACNNNNGTAYC	6 cut(s)	624, 657, 1237, 1270, 1889, 1922
BalI	TGGCCA	2 cut(s)	993, 2079
BamHI	GGATCC	2 cut(s)	857, 2299
BanI	GGYRCC	2 cut(s)	1847, 1905
BauI	CACGAG	2 cut(s)	248, 1580
BbsI	GAAGAC	1 cut(s)	1167
Bbv12I	GWGCWC	1 cut(s)	2015
BbvCI	CCTCAGC	1 cut(s)	2025
BbvI	GCAGC	4 cut(s)	568, 595, 1366, 2269
BccI	CCATC	9 cut(s)	197, 484, 533, 610, 1637, 1896, 2120, 2179, 2290
BceAI	ACGGC	3 cut(s)	337, 341, 2058
BciT130I	CCWGG	4 cut(s)	261, 411, 1613, 1924
BciVI	GTATCC	1 cut(s)	2123
BclI	TGATCA	1 cut(s)	972
BcoDI	GTCTC	4 cut(s)	57, 1211, 1487, 1548
BfaI	CTAG	6 cut(s)	9, 156, 1179, 1380, 1538, 1917
BfmI	CTRYAG	2 cut(s)	609, 909
BfuI	GTATCC	1 cut(s)	2123
BisI	GCNGC	4 cut(s)	582, 609, 1355, 2258
BlnI	CCTAGG	1 cut(s)	1916
BlpI	GCTNAGC	1 cut(s)	13
BlsI	GCNGC	4 cut(s)	583, 610, 1356, 2259
BmcAI	AGTACT	2 cut(s)	163, 1512
Bme1390I	CCNGG	4 cut(s)	261, 411, 1613, 1924
Bme18I	GGWCC	5 cut(s)	141, 917, 1481, 1517, 1920
BmgBI	CACGTC	1 cut(s)	1689
BmgT120I	GGNCC	9 cut(s)	141, 350, 521, 791, 917, 1481, 1517, 1588, 1920
BmiI	GGNNCC	7 cut(s)	142, 523, 859, 1518, 1849, 1907, 2301
BmrFI	CCNGG	4 cut(s)	261, 411, 1613, 1924
BmrI	ACTGGG	2 cut(s)	674, 1523
BmsI	GCATC	7 cut(s)	346, 658, 937, 1318, 1699, 1797, 2272
BmtI	GCTAGC	2 cut(s)	12, 1182
BmuI	ACTGGG	2 cut(s)	674, 1523
BpiI	GAAGAC	1 cut(s)	1167
BpmI	CTGGAG	1 cut(s)	1527
Bpu10I	CCTNAGC	2 cut(s)	1660, 2025
Bpu1102I	GCTNAGC	1 cut(s)	13
BpuEI	CTTGAG	1 cut(s)	383
BsaHI	GRCGYC	1 cut(s)	1655
BsaI	GGTCTC	1 cut(s)	1211
BsaJI	CCNNGG	7 cut(s)	514, 644, 883, 1543, 1612, 1916, 2074
Bsc4I	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1478, 2292
Bse21I	CCTNAGG	1 cut(s)	1069
Bse3DI	GCAATG	4 cut(s)	85, 459, 703, 2267
BseBI	CCWGG	4 cut(s)	261, 411, 1613, 1924
BseDI	CCNNGG	7 cut(s)	514, 644, 883, 1543, 1612, 1916, 2074
BseLI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1478, 2292
BseMI	GCAATG	4 cut(s)	85, 459, 703, 2267
BseMII	CTCAG	9 cut(s)	27, 159, 378, 551, 747, 1374, 1465, 2016, 2058
BseRI	GAGGAG	2 cut(s)	136, 1976
BseSI	GKGCMC	1 cut(s)	2350
BseXI	GCAGC	4 cut(s)	568, 595, 1366, 2269
BseYI	CCCAGC	1 cut(s)	24
BsgI	GTGCAG	1 cut(s)	1855
BshFI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1590, 1858, 2079
BshNI	GGYRCC	2 cut(s)	1847, 1905
BsiHKAI	GWGCWC	1 cut(s)	2015
BslFI	GGGAC	4 cut(s)	496, 1087, 1390, 1530
BslI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1478, 2292
BsmAI	GTCTC	4 cut(s)	57, 1211, 1487, 1548
BsmFI	GGGAC	4 cut(s)	496, 1087, 1390, 1530
BsmI	GAATGC	1 cut(s)	1507
BsnI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1590, 1858, 2079
Bso31I	GGTCTC	1 cut(s)	1211
Bsp1286I	GDGCHC	2 cut(s)	2015, 2350
Bsp143I	GATC	6 cut(s)	857, 972, 1213, 1364, 1773, 2299
Bsp1720I	GCTNAGC	1 cut(s)	13
Bsp19I	CCATGG	2 cut(s)	644, 883
BspACI	CCGC	2 cut(s)	1290, 1664
BspANI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1590, 1858, 2079
BspCNI	CTCAG	9 cut(s)	26, 160, 377, 550, 748, 1373, 1464, 2017, 2059
BspLI	GGNNCC	7 cut(s)	142, 523, 859, 1518, 1849, 1907, 2301
BspMAI	CTGCAG	2 cut(s)	613, 913
BspOI	GCTAGC	2 cut(s)	12, 1182
BspPI	GGATC	6 cut(s)	852, 865, 1372, 1781, 2294, 2307
BspQI	GCTCTTC	1 cut(s)	2082
BspT107I	GGYRCC	2 cut(s)	1847, 1905
BspTNI	GGTCTC	1 cut(s)	1211
BsrDI	GCAATG	4 cut(s)	85, 459, 703, 2267
BssECI	CCNNGG	7 cut(s)	514, 644, 883, 1543, 1612, 1916, 2074
BssMI	GATC	6 cut(s)	857, 972, 1213, 1364, 1773, 2299
BssNI	GRCGYC	1 cut(s)	1655
BssSI	CACGAG	2 cut(s)	248, 1580
BssT1I	CCWWGG	5 cut(s)	514, 644, 883, 1543, 1916
Bst2BI	CACGAG	2 cut(s)	248, 1580
Bst2UI	CCWGG	4 cut(s)	261, 411, 1613, 1924
Bst4CI	ACNGT	9 cut(s)	75, 1060, 1113, 1198, 1312, 1639, 2017, 2120, 2201
Bst6I	CTCTTC	3 cut(s)	657, 1174, 2082
BstACI	GRCGYC	1 cut(s)	1655
BstAPI	GCANNNNNTGC	2 cut(s)	1039, 1833
BstC8I	GCNNGC	7 cut(s)	10, 569, 1035, 1180, 1425, 1834, 2315
BstDSI	CCRYGG	3 cut(s)	644, 883, 2074
BstKTI	GATC	6 cut(s)	860, 975, 1216, 1367, 1776, 2302
BstMAI	GTCTC	4 cut(s)	57, 1211, 1487, 1548
BstMBI	GATC	6 cut(s)	857, 972, 1213, 1364, 1773, 2299
BstNI	CCWGG	4 cut(s)	261, 411, 1613, 1924
BstNSI	RCATGY	5 cut(s)	880, 1340, 1427, 2208, 2317
BstSCI	CCNGG	4 cut(s)	259, 409, 1611, 1922
BstSFI	CTRYAG	2 cut(s)	609, 909
BstSLI	GKGCMC	1 cut(s)	2350
BstV1I	GCAGC	4 cut(s)	568, 595, 1366, 2269
BstV2I	GAAGAC	1 cut(s)	1167
BstX2I	RGATCY	2 cut(s)	857, 2299
BstXI	CCANNNNNNTGG	2 cut(s)	1612, 1689
BstYI	RGATCY	2 cut(s)	857, 2299
Bsu36I	CCTNAGG	1 cut(s)	1069
BsuI	GTATCC	1 cut(s)	2123
BsuRI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1590, 1858, 2079
BtgI	CCRYGG	3 cut(s)	644, 883, 2074
BtrI	CACGTC	1 cut(s)	1689
BtsI	GCAGTG	1 cut(s)	1834
Cac8I	GCNNGC	7 cut(s)	10, 569, 1035, 1180, 1425, 1834, 2315
CaiI	CAGNNNCTG	1 cut(s)	1684
Cfr13I	GGNCC	9 cut(s)	141, 350, 521, 791, 917, 1481, 1517, 1588, 1920
CseI	GACGC	1 cut(s)	1644
Csp6I	GTAC	9 cut(s)	162, 355, 633, 880, 1511, 1848, 1906, 2121, 2202
CspCI	CAANNNNNGTGG	2 cut(s)	187, 222
CviQI	GTAC	9 cut(s)	162, 355, 633, 880, 1511, 1848, 1906, 2121, 2202
DpnI	GATC	6 cut(s)	859, 974, 1215, 1366, 1775, 2301
DpnII	GATC	6 cut(s)	857, 972, 1213, 1364, 1773, 2299
DraI	TTTAAA	1 cut(s)	999
DraIII	CACNNNGTG	1 cut(s)	1585
DrdI	GACNNNNNNGTC	1 cut(s)	1654
DseDI	GACNNNNNNGTC	1 cut(s)	1654
EaeI	YGGCCR	2 cut(s)	991, 2077
Eam1104I	CTCTTC	3 cut(s)	657, 1174, 2082
EarI	CTCTTC	3 cut(s)	657, 1174, 2082
Eco130I	CCWWGG	5 cut(s)	514, 644, 883, 1543, 1916
Eco147I	AGGCCT	1 cut(s)	1858
Eco31I	GGTCTC	1 cut(s)	1211
Eco47I	GGWCC	5 cut(s)	141, 917, 1481, 1517, 1920
Eco57I	CTGAAG	5 cut(s)	406, 1138, 1155, 1737, 1837
Eco81I	CCTNAGG	1 cut(s)	1069
EcoO109I	RGGNCCY	1 cut(s)	1920
EcoRI	GAATTC	1 cut(s)	126
EcoRII	CCWGG	4 cut(s)	259, 409, 1611, 1922
EcoT14I	CCWWGG	5 cut(s)	514, 644, 883, 1543, 1916
EcoT22I	ATGCAT	2 cut(s)	595, 1338
ErhI	CCWWGG	5 cut(s)	514, 644, 883, 1543, 1916
FalI	AAGNNNNNCTT	2 cut(s)	555, 587
FaqI	GGGAC	4 cut(s)	496, 1087, 1390, 1530
FbaI	TGATCA	1 cut(s)	972
Fnu4HI	GCNGC	4 cut(s)	582, 609, 1355, 2258
Fsp4HI	GCNGC	4 cut(s)	582, 609, 1355, 2258
FspBI	CTAG	6 cut(s)	9, 156, 1179, 1380, 1538, 1917
GluI	GCNGC	4 cut(s)	582, 609, 1355, 2258
GsaI	CCCAGC	1 cut(s)	28
GsuI	CTGGAG	1 cut(s)	1527
HaeIII	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1590, 1858, 2079
HgaI	GACGC	1 cut(s)	1644
Hin1I	GRCGYC	1 cut(s)	1655
HincII	GTYRAC	2 cut(s)	1295, 1766
HindII	GTYRAC	2 cut(s)	1295, 1766
HindIII	AAGCTT	1 cut(s)	569
HinfI	GANTC	8 cut(s)	241, 509, 840, 1052, 1235, 1274, 1395, 1672
HphI	GGTGA	4 cut(s)	1219, 1337, 1678, 2033
Hpy166II	GTNNAC	3 cut(s)	777, 1295, 1766
Hpy188I	TCNGA	9 cut(s)	367, 425, 540, 849, 1273, 1308, 1793, 2035, 2281
Hpy188III	TCNNGA	4 cut(s)	167, 1362, 1633, 2175
Hpy8I	GTNNAC	3 cut(s)	777, 1295, 1766
HpyCH4III	ACNGT	9 cut(s)	75, 1060, 1113, 1198, 1312, 1639, 2017, 2120, 2201
HpyCH4IV	ACGT	3 cut(s)	58, 1688, 2002
HpySE526I	ACGT	3 cut(s)	58, 1688, 2002
Hsp92I	GRCGYC	1 cut(s)	1655
KpnI	GGTACC	2 cut(s)	1851, 1909
Ksp22I	TGATCA	1 cut(s)	972
Kzo9I	GATC	6 cut(s)	857, 972, 1213, 1364, 1773, 2299
LguI	GCTCTTC	1 cut(s)	2082
LmnI	GCTCC	3 cut(s)	5, 955, 2218
Lsp1109I	GCAGC	4 cut(s)	568, 595, 1366, 2269
LweI	GCATC	7 cut(s)	346, 658, 937, 1318, 1699, 1797, 2272
MaeI	CTAG	6 cut(s)	9, 156, 1179, 1380, 1538, 1917
MaeII	ACGT	3 cut(s)	58, 1688, 2002
MaeIII	GTNAC	3 cut(s)	1060, 1883, 2207
MalI	GATC	6 cut(s)	859, 974, 1215, 1366, 1775, 2301
MboI	GATC	6 cut(s)	857, 972, 1213, 1364, 1773, 2299
MfeI	CAATTG	1 cut(s)	482
MflI	RGATCY	2 cut(s)	857, 2299
MhlI	GDGCHC	2 cut(s)	2015, 2350
MlsI	TGGCCA	2 cut(s)	993, 2079
MluNI	TGGCCA	2 cut(s)	993, 2079
MlyI	GAGTC	1 cut(s)	518
MmeI	TCCRAC	5 cut(s)	192, 604, 1325, 1444, 1916
Mox20I	TGGCCA	2 cut(s)	993, 2079
Mph1103I	ATGCAT	2 cut(s)	595, 1338
MroXI	GAANNNNTTC	1 cut(s)	1278
MscI	TGGCCA	2 cut(s)	993, 2079
MseI	TTAA	7 cut(s)	111, 306, 998, 1020, 1263, 1928, 2148
MslI	CAYNNNNRTG	2 cut(s)	230, 1687
Msp20I	TGGCCA	2 cut(s)	993, 2079
MspA1I	CMGCKG	2 cut(s)	67, 1322
MspR9I	CCNGG	4 cut(s)	261, 411, 1613, 1924
MunI	CAATTG	1 cut(s)	482
Mva1269I	GAATGC	1 cut(s)	1507
MvaI	CCWGG	4 cut(s)	261, 411, 1613, 1924
NcoI	CCATGG	2 cut(s)	644, 883
NdeII	GATC	6 cut(s)	857, 972, 1213, 1364, 1773, 2299
NheI	GCTAGC	2 cut(s)	8, 1178
NlaIV	GGNNCC	7 cut(s)	142, 523, 859, 1518, 1849, 1907, 2301
NmuCI	GTSAC	1 cut(s)	2207
NsiI	ATGCAT	2 cut(s)	595, 1338
NspI	RCATGY	5 cut(s)	880, 1340, 1427, 2208, 2317
OliI	CACNNNNGTG	2 cut(s)	230, 1687
PaeI	GCATGC	2 cut(s)	1427, 2317
PceI	AGGCCT	1 cut(s)	1858
PciI	ACATGT	2 cut(s)	876, 2204
PciSI	GCTCTTC	1 cut(s)	2082
PctI	GAATGC	1 cut(s)	1507
PdmI	GAANNNNTTC	1 cut(s)	1278
PfeI	GAWTC	7 cut(s)	241, 840, 1052, 1235, 1274, 1395, 1672
PflMI	CCANNNNNTGG	1 cut(s)	750
PkrI	GCNGC	4 cut(s)	583, 610, 1356, 2259
PleI	GAGTC	1 cut(s)	517
PpsI	GAGTC	1 cut(s)	517
PpuMI	RGGWCCY	1 cut(s)	1920
PscI	ACATGT	2 cut(s)	876, 2204
PshBI	ATTAAT	1 cut(s)	111
Psp1406I	AACGTT	1 cut(s)	2002
Psp5II	RGGWCCY	1 cut(s)	1920
Psp6I	CCWGG	4 cut(s)	259, 409, 1611, 1922
PspFI	CCCAGC	1 cut(s)	24
PspGI	CCWGG	4 cut(s)	259, 409, 1611, 1922
PspN4I	GGNNCC	7 cut(s)	142, 523, 859, 1518, 1849, 1907, 2301
PspPI	GGNCC	9 cut(s)	141, 350, 521, 791, 917, 1481, 1517, 1588, 1920
PspPPI	RGGWCCY	1 cut(s)	1920
PstI	CTGCAG	2 cut(s)	613, 913
PstNI	CAGNNNCTG	1 cut(s)	1684
PsuI	RGATCY	2 cut(s)	857, 2299
PvuII	CAGCTG	2 cut(s)	67, 1322
RsaI	GTAC	9 cut(s)	163, 356, 634, 881, 1512, 1849, 1907, 2122, 2203
RsaNI	GTAC	9 cut(s)	162, 355, 633, 880, 1511, 1848, 1906, 2121, 2202
RseI	CAYNNNNRTG	2 cut(s)	230, 1687
SapI	GCTCTTC	1 cut(s)	2082
SaqAI	TTAA	7 cut(s)	111, 306, 998, 1020, 1263, 1928, 2148
SatI	GCNGC	4 cut(s)	582, 609, 1355, 2258
Sau3AI	GATC	6 cut(s)	857, 972, 1213, 1364, 1773, 2299
Sau96I	GGNCC	9 cut(s)	141, 350, 521, 791, 917, 1481, 1517, 1588, 1920
ScaI	AGTACT	2 cut(s)	163, 1512
SchI	GAGTC	1 cut(s)	518
ScrFI	CCNGG	4 cut(s)	261, 411, 1613, 1924
SduI	GDGCHC	2 cut(s)	2015, 2350
SfaNI	GCATC	7 cut(s)	346, 658, 937, 1318, 1699, 1797, 2272
SfcI	CTRYAG	2 cut(s)	609, 909
SinI	GGWCC	5 cut(s)	141, 917, 1481, 1517, 1920
SmiMI	CAYNNNNRTG	2 cut(s)	230, 1687
SmlI	CTYRAG	1 cut(s)	398
SmoI	CTYRAG	1 cut(s)	398
SphI	GCATGC	2 cut(s)	1427, 2317
SseBI	AGGCCT	1 cut(s)	1858
SsiI	CCGC	2 cut(s)	1290, 1664
SspMI	CTAG	6 cut(s)	9, 156, 1179, 1380, 1538, 1917
StuI	AGGCCT	1 cut(s)	1858
StyD4I	CCNGG	4 cut(s)	259, 409, 1611, 1922
StyI	CCWWGG	5 cut(s)	514, 644, 883, 1543, 1916
TaaI	ACNGT	9 cut(s)	75, 1060, 1113, 1198, 1312, 1639, 2017, 2120, 2201
TaiI	ACGT	3 cut(s)	61, 1691, 2005
TaqI	TCGA	3 cut(s)	1257, 1634, 2222
TatI	WGTACW	2 cut(s)	161, 1510
TfiI	GAWTC	7 cut(s)	241, 840, 1052, 1235, 1274, 1395, 1672
Tru1I	TTAA	7 cut(s)	111, 306, 998, 1020, 1263, 1928, 2148
Tru9I	TTAA	7 cut(s)	111, 306, 998, 1020, 1263, 1928, 2148
TseFI	GTSAC	1 cut(s)	2207
TseI	GCWGC	4 cut(s)	581, 608, 1354, 2257
Tsp45I	GTSAC	1 cut(s)	2207
TspGWI	ACGGA	2 cut(s)	1513, 2138
Van91I	CCANNNNNTGG	1 cut(s)	750
VpaK11BI	GGWCC	5 cut(s)	141, 917, 1481, 1517, 1920
VspI	ATTAAT	1 cut(s)	111
XapI	RAATTY	5 cut(s)	126, 373, 461, 782, 2188
XceI	RCATGY	5 cut(s)	880, 1340, 1427, 2208, 2317
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1636, 1899, 2293
XmaJI	CCTAGG	1 cut(s)	1916
XmnI	GAANNNNTTC	1 cut(s)	1278
XspI	CTAG	6 cut(s)	9, 156, 1179, 1380, 1538, 1917
ZrmI	AGTACT	2 cut(s)	163, 1512
Zsp2I	ATGCAT	2 cut(s)	595, 1338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.