Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004743

Physical Location & Seq

Genomic Coordinates Forward (+)

10745331 .. 10749616

Gene Structure 4286 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g12232.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3006 bp

ATGGCCGGAGAAGAAGAAGAGCCGAAAATCCCAGTGTTCACAGTCCTCAAGAACGGAGCGATTCTCAAGAACATGTTCATCGTGAAGCCACCGCCACACTCTCAACCAATCGCCTCCGAAGAAATGTTGATGGTGGGGCGCCACCCCGACTGCCACATCGTGCTGACTCACCCCAGCGTCAGCCGATTCCACCTCCAAATCCTCTCCGATCCTTCCTCGCGCAAGCTCTCTCTCACCGATTTATCATCCGTGCACGGGACTTGGATCTCGGACAAGAGGATTGAGCCAGGGGTTCGAGTTGAGCTGAGGCAAGGCGACACGCTCAGGGTTGGTGCTTCTAGCCGGCTCTATAGGCTTCACTGGATCCCACTCAGCCACGCCTATCAAGACTCACTCAGGCTTCTGAATTCTTCTGCAATGGAGTCTTTGTTCTCTGATGAGCCTCAAGGAGGAGATCACAGTGTTGACAACTCTGAGGCTGTGAATATGTTGCCAATTGAAAACGCCGAATTTGAATCTACTGAATCCATTTCTGAGGTTACAGAATCTCTAGTCTGTGATGATAATTTGGGATTTATTGGGAGGAAGGAGATCCCATCAGCTCCTCCACTATGTGAACATGGTGGCCAGAACTCATCTAAAGTTGTTGGCCAAGAGAACGAATTGTCAACCTTCTGGGCCTTTGGAACTGAATCGGTGAACCTGTTTTATCCTCTTGAATACTCTACTTCTAATTCAGAGGAACACCAGTACCTGCAACTTAACTGTGTCACAGGAGGTTCTGAACTAGATAAGATCCCAGAGAGTTCTTCTTCGCATGAAAAAGGAGAATCTTCTCCTATTGTTCAAGTGTTGGAAAAAGACCAGGGACAGAGTGATATATCTGCTCTTCTTTCAGCTGGACCTTTTGTGAGACAGAATTCATCCTTGCTGGAGACCAAATATCAACAAGTCGAAGAAGATAGCATGAGCCTCGAAACAAATTCAGACTTGCTGGTTAATCTGGATGGCAAAAGCTCTACAACGAAAGACCATGGACAGACTGATCAATTGTGTCTATCTTCTGAACCTCTTCAATATTTTGATGAGAAGGAAGAGGATGCCTGTCCTGCTGCACAAATATCTGGGAGAACTGACGGTCAGAGCTCTTTGAGCAAAGACCATGGACAGACTGATACTGACTTTTGTCTTTCATATGGACCTTCTGTGATGGAGAACTCATCTTTGCTGGTTGGGGAGATCAACAATCAACCAGATGAAGAGGAAAACACAGCACATAAACCAACCTTGTTGGTTAATCTGGATCCTGAGAATACTAATGAAATCAGATGTCTTATTGATGAAAGACATGCAGAAACTGAAAAGAGAAGTGTGTCACCCAAAAACCACCATGAAGAGAGGGATATCAAGACCATCTCCTCTGTGCCTCCTGTCGAAGATTCTACCGACTCATTTTCACCAGATGGGGTTCTCTCAGAGACAATAGATAACAAAGAGAATCAGACTCTGCTATCTATCTGTGTTGTAGTAGGACAGCCTGAATCAGAATTCTGTAAAAGCCCTCCCCTGACATCCGAAGATAAGTCAATCTTTGGAGAAAACATTTGGGCAAGAAGAGGTAAACCTGCTTGTGCTGTTCGTATTCAAACAGAGAGAAGTAGAGAAAGATCAACTGTGGAGGCAGGAATTGAGAAGAATTTTGATCTGGAGGTGGAAATCTTTACTCCAGACAAGGAGAATCTAACACCAAATACAAATACCCTTCGTCTGAGATCTTTGAAAAAGAAGAACGAGGTAGAAGTTAAGCTAGATGATAGTCAAGAGGAGCAGGAAATCTTTAATCCTGACAAGGAAAACTTTACACCAAATACTCTTCAACTGAGGTCCTTGAGGAAGAAGGGTAAGCTAGAATTAACCAATTCCAAATCCCACAGATCGTCTGTGTCGAAGCAAAACTTAATCTCCAATATCCCTAAAGAAGATCTGCTGGTGTGCCCAGGAAAAGATAACCAGAAACTGAAGGATCTCCAAGGAAGACAATCAGTAGAAACTATTGCCAGGAATCAAGCAATGCTGGAAAAAGAGTTGATGCTAACAAAATTAGGCACGGAAAGGATGCCATTCCAATCATTAAAAACTTCTGCAGTCAAGAACAGCAGTTCAGAAGCTTCAGTCTCTGATGTAGCTACTAAAAGCAGCACATCTCTCACTTGTAGTCGAACAAAGGAGCAGGTCAAGAATGCACTCTCTAATAAATTTATTGGAGAAGGAAAGAAGGGTTGGACTATGGTTGCAGACACTAATACTCTGCTTAACAAGGAATCAAGGAAAGCATTGCAACTTCTACAAGGTCTTAAGGGGACTCATTTGATCATTCCAAGAATGGTTATACGGGAACTGGATTGCTTGAAACAGTGTGGCAGCCTGTTTACAAAGAAAACAGAGGCTTGTTTGGTTCTGGAATGGATTGAAGAGTGTATGGTAAAAACAAATTGGTGGATCCATATCCAGAGCTCACTGGAAGATGGAAGGCTGATGGCTCCAACCCCTCCTGCTTCTCCGCGGTCTCTATTTAGTGAGAACGGTTTGGAACTTACTTCTGGAACAACAAGCTCGTTTCCCTTCCTCAGGCGTTGGAGCTCTATGGAACTCGTTTCGCCAGCAGCAGAAGATCATATACTTGATTGTGCTCTTTCCCGTAGGAAGAGGAGCAATGAGCAATTTGTCCTTCTAAGTAATGATATCACCATGAAGATCAAAGCCATGGCAGAGGGTTTGATTTGTGAGACAGCTCAGGAGTTCCATGAGAGTCTAGTGACCCCATCTTCGGAGAGGTTTATGTGGGCTGGTAGCTGTCCCCGCGGGCGTACTTGGTCCTACTTAAATGATGTACTTTTGGCGGGGAAGTATGATCACTGCCAACTGAAGAAGTCATCAAAGGGAGAAGGTGCAAAAGGTCTGAAGCTCATTTTGCTCCACAGTTCTCATTATGGGCAGATCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004540 GO:0004857 GO:0004864 GO:0004865 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006401 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008995 GO:0009056 GO:0009057 GO:0009555 GO:0009892 GO:0009987 GO:0010563 GO:0010605 GO:0010921 GO:0010923 GO:0016070 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0019208 GO:0019212 GO:0019220 GO:0019222 GO:0019439 GO:0019888 GO:0030234 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032515 GO:0034641 GO:0034655 GO:0035303 GO:0035304 GO:0035305 GO:0035308 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043666 GO:0044092 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045936 GO:0046483 GO:0046700 GO:0048229 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0098772 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FHA PIN_4

Protein Analysis

1002

Amino Acids

111.49

Weight (kDa)

5.3

Isoelectric Point (pI)

55.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Yop-YscD_cpl	PF16697	40 - 112	7.7e-09	Inner membrane component of T3SS, cytoplasmic domain
FHA	PF00498	45 - 111	7e-17	FHA domain
PIN_4	PF13638	730 - 898	7.8e-19	PIN domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004743)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34355 AT1G34355 AT1G34355
fragaria_vesca	FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232
malus_domestica	MD05G1040500.v1.1 MD10G1046600.v1.1
prunus_persica	Prupe.8G054200_v2.0.a1
pyrus_communis	pycom05g03310 pycom10g03280 pycom10g03290
rosa_chinensis	RchiOBHm_Chr6g0272171
rosa_laevigata	RLG00000013694
rosa_multiflora	Rmu_sc0005748.1_g000012
rosa_roxburghii	Rroxscaffold_7G00196530
rosa_rugosa	Rorug06G0066700
rosa_samantha	Rh6AG183500 Rh6BG183500 Rh6BG183600 Rh6CG184900 Rh6DG176600
rosa_wichuraiana	Rw6G015820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	762, 1633, 2221
AccB1I	GGYRCC	1 cut(s)	138
AccII	CGCG	3 cut(s)	220, 2563, 2862
AciI	CCGC	6 cut(s)	92, 2561, 2563, 2860, 2862, 2900
AcoI	YGGCCR	3 cut(s)	3, 625, 649
AcsI	RAATTY	7 cut(s)	406, 509, 919, 982, 1547, 1696, 2255
AcuI	CTGAAG	4 cut(s)	2039, 2154, 2945, 2981
AcyI	GRCGYC	1 cut(s)	139
AdeI	CACNNNGTG	2 cut(s)	160, 614
AfaI	GTAC	3 cut(s)	752, 2869, 2892
AfiI	CCNNNNNNNGG	4 cut(s)	255, 449, 2628, 2827
AflII	CTTAAG	1 cut(s)	2354
AflIII	ACRYGT	1 cut(s)	72
AjnI	CCWGG	4 cut(s)	286, 864, 1996, 2057
AjuI	GAANNNNNNNTTGG	2 cut(s)	2263, 2295
AloI	GAACNNNNNNTCC	2 cut(s)	413, 445
Alw21I	GWGCWC	5 cut(s)	255, 1148, 2516, 2642, 2692
Alw26I	GTCTC	6 cut(s)	907, 929, 1472, 2179, 2571, 2780
Alw44I	GTGCAC	1 cut(s)	251
AlwNI	CAGNNNCTG	5 cut(s)	754, 1358, 1507, 2017, 2177
AoxI	GGCC	4 cut(s)	3, 625, 649, 678
ApaLI	GTGCAC	1 cut(s)	251
ApeKI	GCWGC	4 cut(s)	1112, 2196, 2421, 2663
ApoI	RAATTY	7 cut(s)	406, 509, 919, 982, 1547, 1696, 2255
ArsI	GACNNNNNNTTYG	2 cut(s)	2809, 2841
Asp700I	GAANNNNTTC	2 cut(s)	74, 1071
AspLEI	GCGC	2 cut(s)	141, 222
AspS9I	GGNCC	5 cut(s)	678, 902, 1199, 1884, 2874
AsuHPI	GGTGA	6 cut(s)	161, 226, 709, 1368, 1449, 2737
AvaII	GGWCC	4 cut(s)	902, 1199, 1884, 2874
AxyI	CCTNAGG	1 cut(s)	2627
BaeGI	GKGCMC	2 cut(s)	255, 1997
BaeI	ACNNNNGTAYC	2 cut(s)	734, 767
BalI	TGGCCA	2 cut(s)	627, 651
BamHI	GGATCC	3 cut(s)	363, 1303, 2499
BanI	GGYRCC	1 cut(s)	138
BanII	GRGCYC	3 cut(s)	1148, 2516, 2642
BarI	GAAGNNNNNNTAC	2 cut(s)	2661, 2693
BbsI	GAAGAC	1 cut(s)	2041
Bbv12I	GWGCWC	5 cut(s)	255, 1148, 2516, 2642, 2692
BbvCI	CCTCAGC	1 cut(s)	305
BbvI	GCAGC	4 cut(s)	1099, 2208, 2433, 2675
BccI	CCATC	9 cut(s)	124, 604, 1001, 1204, 1421, 1457, 2519, 2530, 2830
BcgI	CGANNNNNNTGC	2 cut(s)	955, 989
BciT130I	CCWGG	4 cut(s)	288, 866, 1998, 2059
BclI	TGATCA	3 cut(s)	1045, 2370, 2911
BcoDI	GTCTC	6 cut(s)	907, 929, 1472, 2179, 2571, 2780
BfaI	CTAG	6 cut(s)	339, 551, 788, 1808, 1907, 2813
BfmI	CTRYAG	2 cut(s)	349, 2142
BfoI	RGCGCY	1 cut(s)	142
BfrI	CTTAAG	1 cut(s)	2354
BfuAI	ACCTGC	3 cut(s)	762, 1633, 2221
BglII	AGATCT	2 cut(s)	1772, 1981
BisI	GCNGC	4 cut(s)	1113, 2197, 2422, 2664
BlsI	GCNGC	4 cut(s)	1114, 2198, 2423, 2665
Bme1390I	CCNGG	4 cut(s)	288, 866, 1998, 2059
Bme18I	GGWCC	4 cut(s)	902, 1199, 1884, 2874
BmgT120I	GGNCC	5 cut(s)	678, 902, 1199, 1884, 2874
BmiI	GGNNCC	5 cut(s)	140, 365, 1305, 2501, 2541
BmrFI	CCNGG	4 cut(s)	288, 866, 1998, 2059
BmrI	ACTGGG	1 cut(s)	26
BmsI	GCATC	3 cut(s)	1090, 2079, 2106
BmuI	ACTGGG	1 cut(s)	26
BoxI	GACNNNNGTC	1 cut(s)	1185
BpiI	GAAGAC	1 cut(s)	2041
BplI	GAGNNNNNCTC	2 cut(s)	48, 80
BpmI	CTGGAG	3 cut(s)	953, 1710, 1727
Bpu10I	CCTNAGC	3 cut(s)	305, 323, 2793
BpuEI	CTTGAG	4 cut(s)	32, 50, 429, 1909
BsaBI	GATNNNNATC	1 cut(s)	2504
BsaHI	GRCGYC	1 cut(s)	139
BsaI	GGTCTC	2 cut(s)	929, 2571
BsaJI	CCNNGG	9 cut(s)	287, 865, 1033, 1162, 1996, 2029, 2561, 2763, 2860
BsaXI	ACNNNNNCTCC	4 cut(s)	413, 443, 1946, 1976
Bsc4I	CCNNNNNNNGG	4 cut(s)	255, 449, 2628, 2827
Bse118I	RCCGGY	1 cut(s)	342
Bse1I	ACTGG	5 cut(s)	32, 365, 748, 2403, 2523
Bse21I	CCTNAGG	1 cut(s)	2627
Bse3DI	GCAATG	4 cut(s)	423, 2076, 2333, 2719
Bse8I	GATNNNNATC	1 cut(s)	2504
BseBI	CCWGG	4 cut(s)	288, 866, 1998, 2059
BseDI	CCNNGG	9 cut(s)	287, 865, 1033, 1162, 1996, 2029, 2561, 2763, 2860
BseGI	GGATG	6 cut(s)	245, 923, 1012, 1105, 1571, 2121
BseJI	GATNNNNATC	1 cut(s)	2504
BseLI	CCNNNNNNNGG	4 cut(s)	255, 449, 2628, 2827
BseMI	GCAATG	4 cut(s)	423, 2076, 2333, 2719
BseNI	ACTGG	5 cut(s)	32, 365, 748, 2403, 2523
BseRI	GAGGAG	5 cut(s)	465, 594, 1408, 1838, 2722
BseSI	GKGCMC	2 cut(s)	255, 1997
BseXI	GCAGC	4 cut(s)	1099, 2208, 2433, 2675
BseYI	CCCAGC	1 cut(s)	173
BsgI	GTGCAG	1 cut(s)	1098
Bsh1236I	CGCG	3 cut(s)	220, 2563, 2862
BshFI	GGCC	4 cut(s)	5, 627, 651, 680
BshNI	GGYRCC	1 cut(s)	138
BsiHKAI	GWGCWC	5 cut(s)	255, 1148, 2516, 2642, 2692
BsiSI	CCGG	2 cut(s)	6, 343
BslFI	GGGAC	4 cut(s)	271, 882, 2374, 2841
BslI	CCNNNNNNNGG	4 cut(s)	255, 449, 2628, 2827
BsmAI	GTCTC	6 cut(s)	907, 929, 1472, 2179, 2571, 2780
BsmFI	GGGAC	4 cut(s)	271, 882, 2374, 2841
BsmI	GAATGC	1 cut(s)	2245
BsnI	GGCC	4 cut(s)	5, 627, 651, 680
Bso31I	GGTCTC	2 cut(s)	929, 2571
Bsp1286I	GDGCHC	6 cut(s)	255, 1148, 1997, 2516, 2642, 2692
Bsp19I	CCATGG	3 cut(s)	1033, 1162, 2763
BspACI	CCGC	6 cut(s)	92, 2561, 2563, 2860, 2862, 2900
BspANI	GGCC	4 cut(s)	5, 627, 651, 680
BspFNI	CGCG	3 cut(s)	220, 2563, 2862
BspLI	GGNNCC	5 cut(s)	140, 365, 1305, 2501, 2541
BspMAI	CTGCAG	1 cut(s)	2146
BspMI	ACCTGC	3 cut(s)	762, 1633, 2221
BspQI	GCTCTTC	2 cut(s)	12, 894
BspT107I	GGYRCC	1 cut(s)	138
BspTI	CTTAAG	1 cut(s)	2354
BspTNI	GGTCTC	2 cut(s)	929, 2571
BsrDI	GCAATG	4 cut(s)	423, 2076, 2333, 2719
BsrFI	RCCGGY	1 cut(s)	342
BsrI	ACTGG	5 cut(s)	32, 365, 748, 2403, 2523
BssAI	RCCGGY	1 cut(s)	342
BssECI	CCNNGG	9 cut(s)	287, 865, 1033, 1162, 1996, 2029, 2561, 2763, 2860
BssNI	GRCGYC	1 cut(s)	139
BssT1I	CCWWGG	4 cut(s)	1033, 1162, 2029, 2763
Bst2UI	CCWGG	4 cut(s)	288, 866, 1998, 2059
Bst4CI	ACNGT	8 cut(s)	43, 461, 767, 1139, 1675, 2415, 2585, 2981
BstACI	GRCGYC	1 cut(s)	139
BstAFI	CTTAAG	1 cut(s)	2354
BstC8I	GCNNGC	4 cut(s)	224, 344, 2661, 2864
BstDSI	CCRYGG	5 cut(s)	1033, 1162, 2561, 2763, 2860
BstENI	CCTNNNNNAGG	2 cut(s)	447, 2626
BstF5I	GGATG	6 cut(s)	245, 923, 1012, 1105, 1571, 2121
BstFNI	CGCG	3 cut(s)	220, 2563, 2862
BstH2I	RGCGCY	1 cut(s)	142
BstHHI	GCGC	2 cut(s)	141, 222
BstMAI	GTCTC	6 cut(s)	907, 929, 1472, 2179, 2571, 2780
BstMWI	GCNNNNNNNGC	5 cut(s)	352, 1109, 1152, 2859, 2971
BstNI	CCWGG	4 cut(s)	288, 866, 1998, 2059
BstNSI	RCATGY	2 cut(s)	76, 1352
BstPAI	GACNNNNGTC	1 cut(s)	1185
BstSCI	CCNGG	4 cut(s)	286, 864, 1996, 2057
BstSFI	CTRYAG	2 cut(s)	349, 2142
BstSLI	GKGCMC	2 cut(s)	255, 1997
BstUI	CGCG	3 cut(s)	220, 2563, 2862
BstV1I	GCAGC	4 cut(s)	1099, 2208, 2433, 2675
BstV2I	GAAGAC	1 cut(s)	2041
Bsu36I	CCTNAGG	1 cut(s)	2627
BsuRI	GGCC	4 cut(s)	5, 627, 651, 680
BtgI	CCRYGG	5 cut(s)	1033, 1162, 2561, 2763, 2860
BtsCI	GGATG	6 cut(s)	245, 923, 1012, 1105, 1571, 2121
BtsI	GCAGTG	1 cut(s)	2914
BtsIMutI	CAGTG	6 cut(s)	39, 358, 466, 2420, 2516, 2914
BveI	ACCTGC	3 cut(s)	762, 1633, 2221
Cac8I	GCNNGC	4 cut(s)	224, 344, 2661, 2864
CaiI	CAGNNNCTG	5 cut(s)	754, 1358, 1507, 2017, 2177
CfoI	GCGC	2 cut(s)	141, 222
Cfr10I	RCCGGY	1 cut(s)	342
Cfr13I	GGNCC	5 cut(s)	678, 902, 1199, 1884, 2874
Cfr42I	CCGCGG	2 cut(s)	2564, 2863
CseI	GACGC	1 cut(s)	166
Csp6I	GTAC	3 cut(s)	751, 2868, 2891
CviQI	GTAC	3 cut(s)	751, 2868, 2891
DinI	GGCGCC	1 cut(s)	140
DraIII	CACNNNGTG	2 cut(s)	160, 614
EaeI	YGGCCR	3 cut(s)	3, 625, 649
Ecl136II	GAGCTC	3 cut(s)	1146, 2514, 2640
Eco130I	CCWWGG	4 cut(s)	1033, 1162, 2029, 2763
Eco24I	GRGCYC	3 cut(s)	1148, 2516, 2642
Eco31I	GGTCTC	2 cut(s)	929, 2571
Eco32I	GATATC	2 cut(s)	1405, 2743
Eco47I	GGWCC	4 cut(s)	902, 1199, 1884, 2874
Eco53kI	GAGCTC	3 cut(s)	1146, 2514, 2640
Eco57I	CTGAAG	4 cut(s)	2039, 2154, 2945, 2981
Eco81I	CCTNAGG	1 cut(s)	2627
EcoICRI	GAGCTC	3 cut(s)	1146, 2514, 2640
EcoNI	CCTNNNNNAGG	2 cut(s)	447, 2626
EcoO109I	RGGNCCY	1 cut(s)	1884
EcoRI	GAATTC	3 cut(s)	406, 919, 1547
EcoRII	CCWGG	4 cut(s)	286, 864, 1996, 2057
EcoRV	GATATC	2 cut(s)	1405, 2743
EcoT14I	CCWWGG	4 cut(s)	1033, 1162, 2029, 2763
EcoT38I	GRGCYC	3 cut(s)	1148, 2516, 2642
EgeI	GGCGCC	1 cut(s)	140
EheI	GGCGCC	1 cut(s)	140
ErhI	CCWWGG	4 cut(s)	1033, 1162, 2029, 2763
FalI	AAGNNNNNCTT	6 cut(s)	1574, 1606, 1841, 1873, 1940, 1972
FaqI	GGGAC	4 cut(s)	271, 882, 2374, 2841
FauI	CCCGC	3 cut(s)	2855, 2867, 2893
FauNDI	CATATG	1 cut(s)	1195
FbaI	TGATCA	3 cut(s)	1045, 2370, 2911
Fnu4HI	GCNGC	4 cut(s)	1113, 2197, 2422, 2664
FokI	GGATG	6 cut(s)	232, 910, 1019, 1112, 1558, 2128
FriOI	GRGCYC	3 cut(s)	1148, 2516, 2642
Fsp4HI	GCNGC	4 cut(s)	1113, 2197, 2422, 2664
FspBI	CTAG	6 cut(s)	339, 551, 788, 1808, 1907, 2813
GlaI	GCGC	2 cut(s)	140, 221
GluI	GCNGC	4 cut(s)	1113, 2197, 2422, 2664
GsaI	CCCAGC	1 cut(s)	177
GsuI	CTGGAG	3 cut(s)	953, 1710, 1727
HaeII	RGCGCY	1 cut(s)	142
HaeIII	GGCC	4 cut(s)	5, 627, 651, 680
HapII	CCGG	2 cut(s)	6, 343
HgaI	GACGC	1 cut(s)	166
HhaI	GCGC	2 cut(s)	141, 222
Hin1I	GRCGYC	1 cut(s)	139
Hin6I	GCGC	2 cut(s)	139, 220
HinP1I	GCGC	2 cut(s)	139, 220
HincII	GTYRAC	2 cut(s)	466, 669
HindII	GTYRAC	2 cut(s)	466, 669
HindIII	AAGCTT	1 cut(s)	2166
HpaII	CCGG	2 cut(s)	6, 343
HphI	GGTGA	6 cut(s)	161, 226, 709, 1368, 1449, 2737
Hpy166II	GTNNAC	8 cut(s)	39, 253, 466, 617, 669, 700, 1622, 2430
Hpy8I	GTNNAC	8 cut(s)	39, 253, 466, 617, 669, 700, 1622, 2430
HpyCH4III	ACNGT	8 cut(s)	43, 461, 767, 1139, 1675, 2415, 2585, 2981
HpyF10VI	GCNNNNNNNGC	5 cut(s)	352, 1109, 1152, 2859, 2971
Hsp92I	GRCGYC	1 cut(s)	139
HspAI	GCGC	2 cut(s)	139, 220
KasI	GGCGCC	1 cut(s)	138
KroI	GCCGGC	1 cut(s)	342
KroNI	GCCGGC	1 cut(s)	344
Ksp22I	TGATCA	3 cut(s)	1045, 2370, 2911
KspI	CCGCGG	2 cut(s)	2564, 2863
LguI	GCTCTTC	2 cut(s)	12, 894
LmnI	GCTCC	8 cut(s)	56, 607, 1825, 2227, 2545, 2637, 2709, 2979
Lsp1109I	GCAGC	4 cut(s)	1099, 2208, 2433, 2675
LweI	GCATC	3 cut(s)	1090, 2079, 2106
MaeI	CTAG	6 cut(s)	339, 551, 788, 1808, 1907, 2813
MaeIII	GTNAC	4 cut(s)	538, 769, 1374, 2815
MfeI	CAATTG	2 cut(s)	495, 1049
MhlI	GDGCHC	6 cut(s)	255, 1148, 1997, 2516, 2642, 2692
MlsI	TGGCCA	2 cut(s)	627, 651
MluNI	TGGCCA	2 cut(s)	627, 651
Mly113I	GGCGCC	1 cut(s)	139
MlyI	GAGTC	7 cut(s)	160, 383, 431, 1442, 1498, 2356, 2818
MmeI	TCCRAC	4 cut(s)	834, 2261, 2567, 2615
Mox20I	TGGCCA	2 cut(s)	627, 651
MroNI	GCCGGC	1 cut(s)	342
MroXI	GAANNNNTTC	2 cut(s)	74, 1071
MscI	TGGCCA	2 cut(s)	627, 651
Msp20I	TGGCCA	2 cut(s)	627, 651
MspA1I	CMGCKG	3 cut(s)	899, 2563, 2862
MspCI	CTTAAG	1 cut(s)	2354
MspI	CCGG	2 cut(s)	6, 343
MspR9I	CCNGG	4 cut(s)	288, 866, 1998, 2059
MunI	CAATTG	2 cut(s)	495, 1049
Mva1269I	GAATGC	1 cut(s)	2245
MvaI	CCWGG	4 cut(s)	288, 866, 1998, 2059
MvnI	CGCG	3 cut(s)	220, 2563, 2862
MwoI	GCNNNNNNNGC	5 cut(s)	352, 1109, 1152, 2859, 2971
NaeI	GCCGGC	1 cut(s)	344
NarI	GGCGCC	1 cut(s)	139
NcoI	CCATGG	3 cut(s)	1033, 1162, 2763
NdeI	CATATG	1 cut(s)	1195
NgoMIV	GCCGGC	1 cut(s)	342
NlaIV	GGNNCC	5 cut(s)	140, 365, 1305, 2501, 2541
NmuCI	GTSAC	3 cut(s)	769, 1374, 2815
NspI	RCATGY	2 cut(s)	76, 1352
PciI	ACATGT	1 cut(s)	72
PciSI	GCTCTTC	2 cut(s)	12, 894
PcsI	WCGNNNNNNNCGW	1 cut(s)	1943
PctI	GAATGC	1 cut(s)	2245
PdiI	GCCGGC	1 cut(s)	344
PdmI	GAANNNNTTC	2 cut(s)	74, 1071
PkrI	GCNGC	4 cut(s)	1114, 2198, 2423, 2665
PleI	GAGTC	7 cut(s)	160, 383, 430, 1442, 1498, 2356, 2817
PluTI	GGCGCC	1 cut(s)	142
PpsI	GAGTC	7 cut(s)	160, 383, 430, 1442, 1498, 2356, 2817
PpuMI	RGGWCCY	1 cut(s)	1884
PscI	ACATGT	1 cut(s)	72
PshAI	GACNNNNGTC	1 cut(s)	1185
Psp124BI	GAGCTC	3 cut(s)	1148, 2516, 2642
Psp5II	RGGWCCY	1 cut(s)	1884
Psp6I	CCWGG	4 cut(s)	286, 864, 1996, 2057
PspFI	CCCAGC	1 cut(s)	173
PspGI	CCWGG	4 cut(s)	286, 864, 1996, 2057
PspN4I	GGNNCC	5 cut(s)	140, 365, 1305, 2501, 2541
PspPI	GGNCC	5 cut(s)	678, 902, 1199, 1884, 2874
PspPPI	RGGWCCY	1 cut(s)	1884
PstI	CTGCAG	1 cut(s)	2146
PstNI	CAGNNNCTG	5 cut(s)	754, 1358, 1507, 2017, 2177
PvuII	CAGCTG	1 cut(s)	899
RsaI	GTAC	3 cut(s)	752, 2869, 2892
RsaNI	GTAC	3 cut(s)	751, 2868, 2891
SacI	GAGCTC	3 cut(s)	1148, 2516, 2642
SacII	CCGCGG	2 cut(s)	2564, 2863
SapI	GCTCTTC	2 cut(s)	12, 894
SatI	GCNGC	4 cut(s)	1113, 2197, 2422, 2664
Sau96I	GGNCC	5 cut(s)	678, 902, 1199, 1884, 2874
SchI	GAGTC	7 cut(s)	160, 383, 431, 1442, 1498, 2356, 2818
ScrFI	CCNGG	4 cut(s)	288, 866, 1998, 2059
SduI	GDGCHC	6 cut(s)	255, 1148, 1997, 2516, 2642, 2692
SfaNI	GCATC	3 cut(s)	1090, 2079, 2106
SfcI	CTRYAG	2 cut(s)	349, 2142
SfoI	GGCGCC	1 cut(s)	140
Sfr303I	CCGCGG	2 cut(s)	2564, 2863
SgrBI	CCGCGG	2 cut(s)	2564, 2863
SinI	GGWCC	4 cut(s)	902, 1199, 1884, 2874
SmlI	CTYRAG	5 cut(s)	47, 65, 444, 1888, 2354
SmoI	CTYRAG	5 cut(s)	47, 65, 444, 1888, 2354
SsiI	CCGC	6 cut(s)	92, 2561, 2563, 2860, 2862, 2900
SspDI	GGCGCC	1 cut(s)	138
SspI	AATATT	1 cut(s)	1079
SspMI	CTAG	6 cut(s)	339, 551, 788, 1808, 1907, 2813
SstI	GAGCTC	3 cut(s)	1148, 2516, 2642
StyD4I	CCNGG	4 cut(s)	286, 864, 1996, 2057
StyI	CCWWGG	4 cut(s)	1033, 1162, 2029, 2763
TaaI	ACNGT	8 cut(s)	43, 461, 767, 1139, 1675, 2415, 2585, 2981
TaqI	TCGA	6 cut(s)	295, 954, 975, 1434, 1946, 2218
TatI	WGTACW	1 cut(s)	2890
TscAI	CASTG	6 cut(s)	39, 365, 466, 2420, 2523, 2921
TseFI	GTSAC	3 cut(s)	769, 1374, 2815
TseI	GCWGC	4 cut(s)	1112, 2196, 2421, 2663
Tsp45I	GTSAC	3 cut(s)	769, 1374, 2815
TspDTI	ATGAA	9 cut(s)	67, 834, 912, 1182, 1272, 1335, 1356, 1407, 2765
TspGWI	ACGGA	3 cut(s)	69, 238, 2123
TspRI	CASTG	6 cut(s)	39, 365, 466, 2420, 2523, 2921
Vha464I	CTTAAG	1 cut(s)	2354
VneI	GTGCAC	1 cut(s)	251
VpaK11BI	GGWCC	4 cut(s)	902, 1199, 1884, 2874
XagI	CCTNNNNNAGG	2 cut(s)	447, 2626
XapI	RAATTY	7 cut(s)	406, 509, 919, 982, 1547, 1696, 2255
XceI	RCATGY	2 cut(s)	76, 1352
XmnI	GAANNNNTTC	2 cut(s)	74, 1071
XspI	CTAG	6 cut(s)	339, 551, 788, 1808, 1907, 2813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.