Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004743

Physical Location & Seq

Genomic Coordinates Forward (+)

6256725 .. 6260816

Gene Structure 4092 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1046600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3117 bp

ATGGCGAATGAGAAAATGAATAGGAAGCTGAACGGAGAAGGAAGGGAAGAAGAAGAGGAGACGAAAATCCCAGTCTTCACAGTCCTCAAGAACGGAGCCATTCTCAAGAACATCTTCATCGTCAACAAATCCCCGCCACCGCCGCACTCCAAACCCATCTCCGCCGTCTACCGAAAAACCCATGAAGAAATCTTGATCGTCGGCCGACACCCGGATTGCAACATCGTCTTGACTCACCCCAGCATCAGCAGATTCCACCTCCAAATCCTCTCCGACCCTTTTTCTCAAAAGCTCTCTCTCACCGATTTGTCATCAGTACATGGGACTTGGGTTTCGGACAAGAAGATCGAGCCGGGAGTTCGAGTGGAGCTGAGCGAAGGGGACACGCTCAGGCTTGGTGGTTCCAGCAGGGTCTACAGCTTGCATTGGATTCCTATGAGGCAGGCCTATGATTTCGAAACACCTTTCGTGCCATTCATAGCGCACGACGATGATGAAAATGCAGAACAAGTATCGGATCAGAGTGAGAATTCTTTGCCTGTTGAGAACAAAGAAACTGAATCTCCAGATTCAAATTCCGTGGGTGTAGAATCATTGTTCTCTGATGAAATTGTGGGAGTCATTGGGAAGATGGAGGTCCCATCAGCACCTCCACTGCCGGAAAATGCCGTTTACTCAATTTTTGATCAAGGCGAAGAATGGGGCGAGAAATTATCAAAAGGTAGTGGTGACGAGAATGAAGTCTCAAGTTTCTGGGCATTTGGAACGGAATCGGTGAACCTGTTTTTGGAGATGGAAGAATCTAATCCTAATCGAATGGAAAACCAGCGCCTACAACCTCACTGTGTCACAGAAGAGGATTCGGAAAGAGAGAACCTAGAGAGTTCCTTTTTTACTGCAGAGGAAGGGGAAGCTTGTCTTGCTGTGCAAGTGTCAGAGGAAAGTGGGAAGCAAAGTCAATTGGGAAAAGACCATGGAAATACTAATTTTTTGAGTTCACCTCTTCTGACTGGGGAAATCCTCGAGGAGACCAAAACTCAGCTAGATGAGAAAGAAAACCTGACCCCGGAATCAAAACCAAACTTGCCCGCCAACCTGAATGCTGAACGTTCTTCTGGTGAAAAGGAAGAGGCCGATCCTGCTGCACAAGTACCTGAGAAGAACCAAAGCCCCTTGAGAAAAGATCAGGGACAGTTTGATATTTTATGTCTTTGTTCTGGACCTCAGGTGATGGAGAACTCAACCTTGCCGACTGGAGAATCCCTTGGGGAGGACAAAGATGAGCAAGTTCCAGAACAAAGCCTGACCCCAGAACCAAAATCAAACTTGCTGATTAGACAAAAGTTTGAACATTTTGATGGAAAGGAAAAGGAAGCTCATGCTGCTGCTTCTGTACCTGATAAAATTGAGAACCAACGCCCACTGAGAAAAGATAATGGACAGACTGATATTTCATGTCTCTTTTCTGGACCCCTTGAAATGGAGAACTTATTGTTGCCAATCGGAGAAGTCCTCGGGGAGACCAAAGATCAGAGAGTTGTAGAAGAAAGCCTGACCCTAGAACCAAAATCCAACTTGCCTGTCAACCTGAACTTTGAGCATTCTGATGAAAAAGAATGTCTAGTGGATGTGAGTTGTGGAGAATCGGAAAACAAAAGTGTGGCATCAGAAGATCATGAAAAAAGGGATACAAGCATTTCTTCTGCACCTCTTGTGACAGAATCTGGAACCTCATCTATATCAGAGTTAATAAATAACAAGGAGAGGCAGACCCCACAATCTCTCTTTACTGCAGCAGGACAGCCTGAATCGAAACTCTGTGAAAGCCCTCCACTGAGATCCGAGAATAAATCAAGTACGATGGTGGGAAGCATTTGGGCAAGAAGAGGTAAACCTGCTAGTGCGGTAAAGCTTCAAACAGATAAGAGCAGAGGAAAACCTGAGGCTGCGTATGATGATGATATTGGACAGGAGGAGATCTTTACTCCAGACAAGGAAAATTTCACCCCAAATACTCTTCGACTGAGGTCTTTGAAAAAGAAGGGTAAGATAGAAGTTAAGCATTCCAAATCCAGCACATCATCCTCGTCAAAACTAACTCTAGTCTCCAATATCCATCAACAAGAACTGATTGAATCCCCAGGAAAAGAGAACCAGATAATGAAGGAACTCCAAGAAACAAAATTACCTGGAAACACTTCTGGAAATCAAGCTAGGGTGGAGGAAAAGTTGACGGTAACAAAAGTAAGAAGAGAAAGGATTCCTTTTCAATCAATCAAACGTTCTGGAGGCAAGAACATATCAGAAGACTCAGTCCCTAACACAGCGACAAGAAGCAGTACATATTTCAGTTGTACTCAAAATAAGGAGGTCGCCAATGCATGCTCTAATAAATCTGTTGGAGAAGGAAAGAGGAGCTGGACTATGGTTGCGGACGCTACCACTCTTCTCGACAAGGAATCAAGGAAGTCATTGCAGTTTCTACAAGTCATACGGGAACTGGATTGCTTGAAGCAACGTGGCAGCCTTTTCAGAAAGAAAACAGAGGCTGAATTGGTGCTGGAATGGATCAAAGACTGTATGATTAAAACAAATTGGTGGATCCATGTCCAGAGCTCAATGGAAGACGGAAGACTGATTGCTCCAACCCCTCCTGCTTCGCCGCAGTCTCTATTTAGCGAGAAGAGTTGGGGCTTTCCTTCTAGGACAACGGGCTCATTGACTTTTTCGAGGTGTGGGAGCATGATGGACCTTGTATCACCGTCCCCAGAAGACCATATCCTAGATTGTGCTCTTTTATGTAGAAGGATGAAGAGGAACGATGGACAACTTGTCCTTCTCAGTACCGATGTCACCCTGAAGATCAAAGCTATGGAAGAGGGTTTGCTCTGTGAGACAGCTGAAGAATTTCGTGAGGGTCTGGTTAACCCACTTTCTGAGAGGTTTTTGTGGCCCGACAGTTCTCCCTGTGGGCGTACATGGTCTTACTTAGGAAACGGAGCTTTGAGAGAAAAGTACAATAGCTGCGGCCCTCTGAAGAAGTCGTCAAAAGGAGAAAGTGCAAAGGGTTTGAAGCTCATTCTGCACCATAATGCTCATTACGGGCAGATCCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004540 GO:0004857 GO:0004864 GO:0004865 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006401 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008995 GO:0009056 GO:0009057 GO:0009555 GO:0009892 GO:0009987 GO:0010563 GO:0010605 GO:0010921 GO:0010923 GO:0016070 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0019208 GO:0019212 GO:0019220 GO:0019222 GO:0019439 GO:0019888 GO:0030234 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032515 GO:0034641 GO:0034655 GO:0035303 GO:0035304 GO:0035305 GO:0035308 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043666 GO:0044092 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045936 GO:0046483 GO:0046700 GO:0048229 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0098772 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FHA PIN_4

Protein Analysis

1039

Amino Acids

115.44

Weight (kDa)

5.18

Isoelectric Point (pI)

57.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Yop-YscD_cpl	PF16697	61 - 133	1.6e-08	Inner membrane component of T3SS, cytoplasmic domain
FHA	PF00498	66 - 133	1.4e-16	FHA domain
PIN_4	PF13638	829 - 967	1.6e-11	PIN domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004743)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34355 AT1G34355 AT1G34355
fragaria_vesca	FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232
malus_domestica	MD05G1040500.v1.1 MD10G1046600.v1.1
prunus_persica	Prupe.8G054200_v2.0.a1
pyrus_communis	pycom05g03310 pycom10g03280 pycom10g03290
rosa_chinensis	RchiOBHm_Chr6g0272171
rosa_laevigata	RLG00000013694
rosa_multiflora	Rmu_sc0005748.1_g000012
rosa_roxburghii	Rroxscaffold_7G00196530
rosa_rugosa	Rorug06G0066700
rosa_samantha	Rh6AG183500 Rh6BG183500 Rh6BG183600 Rh6CG184900 Rh6DG176600
rosa_wichuraiana	Rw6G015820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1022
Acc36I	ACCTGC	1 cut(s)	1903
AccI	GTMKAC	2 cut(s)	168, 414
AciI	CCGC	9 cut(s)	134, 140, 143, 162, 1089, 1904, 2432, 2663, 3027
AclI	AACGTT	2 cut(s)	1108, 2281
AclWI	GGATC	7 cut(s)	525, 1130, 1833, 2576, 2596, 2609, 3103
AcoI	YGGCCR	1 cut(s)	202
AcsI	RAATTY	4 cut(s)	529, 574, 1999, 2906
AcuI	CTGAAG	3 cut(s)	2879, 2922, 3056
AfaI	GTAC	9 cut(s)	318, 1152, 1395, 1858, 2341, 2356, 2845, 2977, 3017
AfiI	CCNNNNNNNGG	5 cut(s)	211, 787, 1066, 1270, 1480
AgsI	TTSAA	9 cut(s)	573, 1349, 1478, 1916, 2035, 2135, 2270, 2512, 3073
AhdI	GACNNNNNGTC	1 cut(s)	2831
AjnI	CCWGG	2 cut(s)	2140, 2188
AjuI	GAANNNNNNNTTGG	2 cut(s)	770, 802
Alw21I	GWGCWC	2 cut(s)	2618, 2794
Alw26I	GTCTC	8 cut(s)	53, 748, 1022, 1463, 1514, 2110, 2673, 2888
AlwI	GGATC	7 cut(s)	525, 1130, 1833, 2576, 2596, 2609, 3103
AlwNI	CAGNNNCTG	2 cut(s)	1724, 2549
Ama87I	CYCGRG	2 cut(s)	1022, 1514
AoxI	GGCC	5 cut(s)	202, 444, 1131, 2951, 3028
ApeKI	GCWGC	7 cut(s)	1142, 1382, 1385, 1793, 1946, 2523, 3024
ApoI	RAATTY	4 cut(s)	529, 574, 1999, 2906
ArsI	GACNNNNNNTTYG	2 cut(s)	316, 348
Asp700I	GAANNNNTTC	4 cut(s)	113, 2197, 2259, 2907
AspLEI	GCGC	2 cut(s)	484, 831
AspS9I	GGNCC	6 cut(s)	637, 1220, 1469, 2749, 2952, 3029
AsuC2I	CCSGG	3 cut(s)	212, 354, 1067
AsuII	TTCGAA	1 cut(s)	456
AvaI	CYCGRG	2 cut(s)	1022, 1514
AvaII	GGWCC	4 cut(s)	637, 1220, 1469, 2749
AxyI	CCTNAGG	2 cut(s)	1224, 1941
BamHI	GGATCC	1 cut(s)	2601
BanII	GRGCYC	2 cut(s)	2618, 2717
BbsI	GAAGAC	5 cut(s)	67, 2313, 2631, 2638, 2778
Bbv12I	GWGCWC	2 cut(s)	2618, 2794
BbvI	GCAGC	7 cut(s)	1129, 1369, 1372, 1805, 1933, 2535, 3011
BceAI	ACGGC	2 cut(s)	149, 653
BcgI	CGANNNNNNTGC	2 cut(s)	1124, 1158
BciT130I	CCWGG	2 cut(s)	2142, 2190
BciVI	GTATCC	1 cut(s)	1681
BclI	TGATCA	1 cut(s)	685
BcnI	CCSGG	3 cut(s)	212, 354, 1067
BcoDI	GTCTC	8 cut(s)	53, 748, 1022, 1463, 1514, 2110, 2673, 2888
BfaI	CTAG	9 cut(s)	878, 1043, 1559, 1622, 1899, 2102, 2214, 2703, 2783
BfmI	CTRYAG	3 cut(s)	415, 897, 1791
BfoI	RGCGCY	1 cut(s)	832
BfuAI	ACCTGC	1 cut(s)	1903
BfuI	GTATCC	1 cut(s)	1681
BglII	AGATCT	1 cut(s)	1977
BlpI	GCTNAGC	1 cut(s)	371
Bme1390I	CCNGG	5 cut(s)	212, 354, 1067, 2142, 2190
Bme18I	GGWCC	4 cut(s)	637, 1220, 1469, 2749
BmeRI	GACNNNNNGTC	1 cut(s)	2831
BmeT110I	CYCGRG	2 cut(s)	1022, 1514
BmgT120I	GGNCC	6 cut(s)	637, 1220, 1469, 2749, 2952, 3029
BmiI	GGNNCC	6 cut(s)	97, 403, 639, 1471, 1729, 2603
BmrFI	CCNGG	5 cut(s)	212, 354, 1067, 2142, 2190
BmrI	ACTGGG	2 cut(s)	65, 1020
BmsI	GCATC	2 cut(s)	252, 1673
BmuI	ACTGGG	2 cut(s)	65, 1020
BoxI	GACNNNNGTC	1 cut(s)	2026
BpiI	GAAGAC	5 cut(s)	67, 2313, 2631, 2638, 2778
BplI	GAGNNNNNCTC	6 cut(s)	87, 119, 985, 1017, 1497, 1529
BpmI	CTGGAG	4 cut(s)	549, 1275, 1971, 2307
Bpu10I	CCTNAGC	1 cut(s)	389
Bpu1102I	GCTNAGC	1 cut(s)	371
Bpu14I	TTCGAA	1 cut(s)	456
BpuEI	CTTGAG	4 cut(s)	71, 89, 730, 1195
BpuMI	CCSGG	3 cut(s)	212, 354, 1067
BsaI	GGTCTC	2 cut(s)	1022, 1514
BsaJI	CCNNGG	6 cut(s)	579, 973, 1065, 1264, 1513, 2140
BsaXI	ACNNNNNCTCC	6 cut(s)	348, 378, 547, 577, 2213, 2243
Bsc4I	CCNNNNNNNGG	5 cut(s)	211, 787, 1066, 1270, 1480
Bse1I	ACTGG	4 cut(s)	71, 1015, 1258, 2505
Bse21I	CCTNAGG	2 cut(s)	1224, 1941
Bse3DI	GCAATG	1 cut(s)	2471
BseBI	CCWGG	2 cut(s)	2142, 2190
BseDI	CCNNGG	6 cut(s)	579, 973, 1065, 1264, 1513, 2140
BseGI	GGATG	3 cut(s)	1633, 2081, 2814
BseLI	CCNNNNNNNGG	5 cut(s)	211, 787, 1066, 1270, 1480
BseMI	GCAATG	1 cut(s)	2471
BseNI	ACTGG	4 cut(s)	71, 1015, 1258, 2505
BseRI	GAGGAG	4 cut(s)	71, 1040, 1988, 2428
BseX3I	CGGCCG	1 cut(s)	202
BseXI	GCAGC	7 cut(s)	1129, 1369, 1372, 1805, 1933, 2535, 3011
BseYI	CCCAGC	1 cut(s)	239
BsgI	GTGCAG	3 cut(s)	1128, 1689, 3068
Bsh1285I	CGRYCG	1 cut(s)	205
BshFI	GGCC	5 cut(s)	204, 446, 1133, 2953, 3030
BsiEI	CGRYCG	1 cut(s)	205
BsiHKAI	GWGCWC	2 cut(s)	2618, 2794
BsiHKCI	CYCGRG	2 cut(s)	1022, 1514
BsiSI	CCGG	4 cut(s)	212, 353, 659, 1067
BslFI	GGGAC	6 cut(s)	337, 395, 623, 1203, 2300, 2749
BslI	CCNNNNNNNGG	5 cut(s)	211, 787, 1066, 1270, 1480
BsmAI	GTCTC	8 cut(s)	53, 748, 1022, 1463, 1514, 2110, 2673, 2888
BsmBI	CGTCTC	1 cut(s)	53
BsmFI	GGGAC	6 cut(s)	337, 395, 623, 1203, 2300, 2749
BsmI	GAATGC	3 cut(s)	1105, 1600, 2062
BsnI	GGCC	5 cut(s)	204, 446, 1133, 2953, 3030
Bso31I	GGTCTC	2 cut(s)	1022, 1514
BsoBI	CYCGRG	2 cut(s)	1022, 1514
Bsp119I	TTCGAA	1 cut(s)	456
Bsp1286I	GDGCHC	3 cut(s)	2618, 2717, 2794
Bsp1720I	GCTNAGC	1 cut(s)	371
Bsp19I	CCATGG	1 cut(s)	973
BspACI	CCGC	9 cut(s)	134, 140, 143, 162, 1089, 1904, 2432, 2663, 3027
BspANI	GGCC	5 cut(s)	204, 446, 1133, 2953, 3030
BspHI	TCATGA	1 cut(s)	1675
BspLI	GGNNCC	6 cut(s)	97, 403, 639, 1471, 1729, 2603
BspMAI	CTGCAG	2 cut(s)	901, 1795
BspMI	ACCTGC	1 cut(s)	1903
BspPI	GGATC	7 cut(s)	525, 1130, 1833, 2576, 2596, 2609, 3103
BspT104I	TTCGAA	1 cut(s)	456
BspTNI	GGTCTC	2 cut(s)	1022, 1514
BsrDI	GCAATG	1 cut(s)	2471
BsrI	ACTGG	4 cut(s)	71, 1015, 1258, 2505
BssECI	CCNNGG	6 cut(s)	579, 973, 1065, 1264, 1513, 2140
BssT1I	CCWWGG	2 cut(s)	973, 1264
Bst2UI	CCWGG	2 cut(s)	2142, 2190
Bst4CI	ACNGT	7 cut(s)	82, 845, 1194, 2236, 2579, 2763, 2960
BstBI	TTCGAA	1 cut(s)	456
BstC8I	GCNNGC	4 cut(s)	422, 444, 1089, 2383
BstDSI	CCRYGG	2 cut(s)	579, 973
BstENI	CCTNNNNNAGG	1 cut(s)	1268
BstF5I	GGATG	3 cut(s)	1633, 2081, 2814
BstH2I	RGCGCY	1 cut(s)	832
BstHHI	GCGC	2 cut(s)	484, 831
BstMAI	GTCTC	8 cut(s)	53, 748, 1022, 1463, 1514, 2110, 2673, 2888
BstMCI	CGRYCG	1 cut(s)	205
BstMWI	GCNNNNNNNGC	5 cut(s)	142, 920, 1139, 1382, 3082
BstNI	CCWGG	2 cut(s)	2142, 2190
BstNSI	RCATGY	1 cut(s)	2385
BstPAI	GACNNNNGTC	1 cut(s)	2026
BstSCI	CCNGG	5 cut(s)	210, 352, 1065, 2140, 2188
BstSFI	CTRYAG	3 cut(s)	415, 897, 1791
BstV1I	GCAGC	7 cut(s)	1129, 1369, 1372, 1805, 1933, 2535, 3011
BstV2I	GAAGAC	5 cut(s)	67, 2313, 2631, 2638, 2778
BstX2I	RGATCY	4 cut(s)	1838, 1977, 2601, 3108
BstYI	RGATCY	4 cut(s)	1838, 1977, 2601, 3108
BstZI	CGGCCG	1 cut(s)	202
Bsu36I	CCTNAGG	2 cut(s)	1224, 1941
BsuI	GTATCC	1 cut(s)	1681
BsuRI	GGCC	5 cut(s)	204, 446, 1133, 2953, 3030
BtgI	CCRYGG	2 cut(s)	579, 973
BtsCI	GGATG	3 cut(s)	1633, 2081, 2814
BtsI	GCAGTG	1 cut(s)	653
BtsIMutI	CAGTG	4 cut(s)	653, 841, 1421, 1832
BveI	ACCTGC	1 cut(s)	1903
Cac8I	GCNNGC	4 cut(s)	422, 444, 1089, 2383
CaiI	CAGNNNCTG	2 cut(s)	1724, 2549
CciI	TCATGA	1 cut(s)	1675
CfoI	GCGC	2 cut(s)	484, 831
Cfr13I	GGNCC	6 cut(s)	637, 1220, 1469, 2749, 2952, 3029
CseI	GACGC	1 cut(s)	2444
Csp6I	GTAC	9 cut(s)	317, 1151, 1394, 1857, 2340, 2355, 2844, 2976, 3016
CspCI	CAANNNNNGTGG	2 cut(s)	561, 596
CviQI	GTAC	9 cut(s)	317, 1151, 1394, 1857, 2340, 2355, 2844, 2976, 3016
DriI	GACNNNNNGTC	1 cut(s)	2831
EaeI	YGGCCR	1 cut(s)	202
EagI	CGGCCG	1 cut(s)	202
Eam1105I	GACNNNNNGTC	1 cut(s)	2831
EciI	GGCGGA	1 cut(s)	151
Ecl136II	GAGCTC	1 cut(s)	2616
EclXI	CGGCCG	1 cut(s)	202
Eco130I	CCWWGG	2 cut(s)	973, 1264
Eco147I	AGGCCT	1 cut(s)	446
Eco24I	GRGCYC	2 cut(s)	2618, 2717
Eco31I	GGTCTC	2 cut(s)	1022, 1514
Eco47I	GGWCC	4 cut(s)	637, 1220, 1469, 2749
Eco52I	CGGCCG	1 cut(s)	202
Eco53kI	GAGCTC	1 cut(s)	2616
Eco57I	CTGAAG	3 cut(s)	2879, 2922, 3056
Eco81I	CCTNAGG	2 cut(s)	1224, 1941
Eco88I	CYCGRG	2 cut(s)	1022, 1514
EcoICRI	GAGCTC	1 cut(s)	2616
EcoNI	CCTNNNNNAGG	1 cut(s)	1268
EcoO109I	RGGNCCY	1 cut(s)	637
EcoRI	GAATTC	1 cut(s)	529
EcoRII	CCWGG	2 cut(s)	2140, 2188
EcoT14I	CCWWGG	2 cut(s)	973, 1264
EcoT22I	ATGCAT	1 cut(s)	2383
EcoT38I	GRGCYC	2 cut(s)	2618, 2717
ErhI	CCWWGG	2 cut(s)	973, 1264
Esp3I	CGTCTC	1 cut(s)	53
FalI	AAGNNNNNCTT	8 cut(s)	98, 130, 903, 935, 1684, 1716, 2248, 2280
FaqI	GGGAC	6 cut(s)	337, 395, 623, 1203, 2300, 2749
FauI	CCCGC	2 cut(s)	141, 1096
FbaI	TGATCA	1 cut(s)	685
FblI	GTMKAC	2 cut(s)	168, 414
FokI	GGATG	3 cut(s)	1640, 2068, 2821
FriOI	GRGCYC	2 cut(s)	2618, 2717
FspBI	CTAG	9 cut(s)	878, 1043, 1559, 1622, 1899, 2102, 2214, 2703, 2783
GlaI	GCGC	2 cut(s)	483, 830
GsaI	CCCAGC	1 cut(s)	243
GsuI	CTGGAG	4 cut(s)	549, 1275, 1971, 2307
HaeII	RGCGCY	1 cut(s)	832
HaeIII	GGCC	5 cut(s)	204, 446, 1133, 2953, 3030
HapII	CCGG	4 cut(s)	212, 353, 659, 1067
HgaI	GACGC	1 cut(s)	2444
HhaI	GCGC	2 cut(s)	484, 831
Hin6I	GCGC	2 cut(s)	482, 829
HinP1I	GCGC	2 cut(s)	482, 829
HincII	GTYRAC	4 cut(s)	124, 1585, 2232, 2926
HindII	GTYRAC	4 cut(s)	124, 1585, 2232, 2926
HindIII	AAGCTT	2 cut(s)	912, 1910
HpaI	GTTAAC	1 cut(s)	2926
HpaII	CCGG	4 cut(s)	212, 353, 659, 1067
Hpy99I	CGWCG	2 cut(s)	203, 491
HpyCH4III	ACNGT	7 cut(s)	82, 845, 1194, 2236, 2579, 2763, 2960
HpyCH4IV	ACGT	3 cut(s)	1108, 2281, 2518
HpyF10VI	GCNNNNNNNGC	5 cut(s)	142, 920, 1139, 1382, 3082
HpySE526I	ACGT	3 cut(s)	1108, 2281, 2518
HspAI	GCGC	2 cut(s)	482, 829
Ksp22I	TGATCA	1 cut(s)	685
KspAI	GTTAAC	1 cut(s)	2926
LmnI	GCTCC	6 cut(s)	95, 367, 2415, 2647, 2739, 2999
Lsp1109I	GCAGC	7 cut(s)	1129, 1369, 1372, 1805, 1933, 2535, 3011
LweI	GCATC	2 cut(s)	252, 1673
MaeI	CTAG	9 cut(s)	878, 1043, 1559, 1622, 1899, 2102, 2214, 2703, 2783
MaeII	ACGT	3 cut(s)	1108, 2281, 2518
MaeIII	GTNAC	5 cut(s)	728, 847, 1714, 2236, 2851
MfeI	CAATTG	1 cut(s)	959
MflI	RGATCY	4 cut(s)	1838, 1977, 2601, 3108
MhlI	GDGCHC	3 cut(s)	2618, 2717, 2794
MlyI	GAGTC	3 cut(s)	226, 627, 2303
MmeI	TCCRAC	4 cut(s)	297, 1596, 2380, 2669
Mph1103I	ATGCAT	1 cut(s)	2383
MroXI	GAANNNNTTC	4 cut(s)	113, 2197, 2259, 2907
MseI	TTAA	4 cut(s)	1748, 2058, 2586, 2925
MslI	CAYNNNNRTG	4 cut(s)	489, 1356, 1605, 3090
MspA1I	CMGCKG	1 cut(s)	2900
MspI	CCGG	4 cut(s)	212, 353, 659, 1067
MspR9I	CCNGG	5 cut(s)	212, 354, 1067, 2142, 2190
MunI	CAATTG	1 cut(s)	959
Mva1269I	GAATGC	3 cut(s)	1105, 1600, 2062
MvaI	CCWGG	2 cut(s)	2142, 2190
MwoI	GCNNNNNNNGC	5 cut(s)	142, 920, 1139, 1382, 3082
NciI	CCSGG	3 cut(s)	212, 354, 1067
NcoI	CCATGG	1 cut(s)	973
NlaIV	GGNNCC	6 cut(s)	97, 403, 639, 1471, 1729, 2603
NmuCI	GTSAC	4 cut(s)	728, 847, 1714, 2851
NsiI	ATGCAT	1 cut(s)	2383
NspI	RCATGY	1 cut(s)	2385
NspV	TTCGAA	1 cut(s)	456
PaeI	GCATGC	1 cut(s)	2385
PaeR7I	CTCGAG	1 cut(s)	1022
PagI	TCATGA	1 cut(s)	1675
PceI	AGGCCT	1 cut(s)	446
PctI	GAATGC	3 cut(s)	1105, 1600, 2062
PdmI	GAANNNNTTC	4 cut(s)	113, 2197, 2259, 2907
PflFI	GACNNNGTC	1 cut(s)	2312
PleI	GAGTC	3 cut(s)	226, 626, 2303
PpsI	GAGTC	3 cut(s)	226, 626, 2303
PpuMI	RGGWCCY	1 cut(s)	637
PshAI	GACNNNNGTC	1 cut(s)	2026
Psp124BI	GAGCTC	1 cut(s)	2618
Psp1406I	AACGTT	2 cut(s)	1108, 2281
Psp5II	RGGWCCY	1 cut(s)	637
Psp6I	CCWGG	2 cut(s)	2140, 2188
PspFI	CCCAGC	1 cut(s)	239
PspGI	CCWGG	2 cut(s)	2140, 2188
PspN4I	GGNNCC	6 cut(s)	97, 403, 639, 1471, 1729, 2603
PspPI	GGNCC	6 cut(s)	637, 1220, 1469, 2749, 2952, 3029
PspPPI	RGGWCCY	1 cut(s)	637
PspXI	VCTCGAGB	1 cut(s)	1022
PstI	CTGCAG	2 cut(s)	901, 1795
PstNI	CAGNNNCTG	2 cut(s)	1724, 2549
PsuI	RGATCY	4 cut(s)	1838, 1977, 2601, 3108
PsyI	GACNNNGTC	1 cut(s)	2312
PvuII	CAGCTG	1 cut(s)	2900
RsaI	GTAC	9 cut(s)	318, 1152, 1395, 1858, 2341, 2356, 2845, 2977, 3017
RsaNI	GTAC	9 cut(s)	317, 1151, 1394, 1857, 2340, 2355, 2844, 2976, 3016
RseI	CAYNNNNRTG	4 cut(s)	489, 1356, 1605, 3090
SacI	GAGCTC	1 cut(s)	2618
SaqAI	TTAA	4 cut(s)	1748, 2058, 2586, 2925
Sau96I	GGNCC	6 cut(s)	637, 1220, 1469, 2749, 2952, 3029
SchI	GAGTC	3 cut(s)	226, 627, 2303
ScrFI	CCNGG	5 cut(s)	212, 354, 1067, 2142, 2190
SduI	GDGCHC	3 cut(s)	2618, 2717, 2794
SfaNI	GCATC	2 cut(s)	252, 1673
SfcI	CTRYAG	3 cut(s)	415, 897, 1791
Sfr274I	CTCGAG	1 cut(s)	1022
SfuI	TTCGAA	1 cut(s)	456
SinI	GGWCC	4 cut(s)	637, 1220, 1469, 2749
SlaI	CTCGAG	1 cut(s)	1022
SmiMI	CAYNNNNRTG	4 cut(s)	489, 1356, 1605, 3090
SmlI	CTYRAG	5 cut(s)	86, 104, 745, 1022, 1174
SmoI	CTYRAG	5 cut(s)	86, 104, 745, 1022, 1174
SphI	GCATGC	1 cut(s)	2385
SseBI	AGGCCT	1 cut(s)	446
SsiI	CCGC	9 cut(s)	134, 140, 143, 162, 1089, 1904, 2432, 2663, 3027
SspMI	CTAG	9 cut(s)	878, 1043, 1559, 1622, 1899, 2102, 2214, 2703, 2783
SstI	GAGCTC	1 cut(s)	2618
StuI	AGGCCT	1 cut(s)	446
StyD4I	CCNGG	5 cut(s)	210, 352, 1065, 2140, 2188
StyI	CCWWGG	2 cut(s)	973, 1264
TaaI	ACNGT	7 cut(s)	82, 845, 1194, 2236, 2579, 2763, 2960
TaiI	ACGT	3 cut(s)	1111, 2284, 2521
TaqI	TCGA	9 cut(s)	348, 361, 456, 814, 1023, 1811, 2020, 2451, 2729
TatI	WGTACW	4 cut(s)	316, 2339, 2354, 3015
TauI	GCSGC	3 cut(s)	145, 2665, 3030
Tru1I	TTAA	4 cut(s)	1748, 2058, 2586, 2925
Tru9I	TTAA	4 cut(s)	1748, 2058, 2586, 2925
TscAI	CASTG	4 cut(s)	660, 848, 1428, 1839
TseFI	GTSAC	4 cut(s)	728, 847, 1714, 2851
TseI	GCWGC	7 cut(s)	1142, 1382, 1385, 1793, 1946, 2523, 3024
Tsp45I	GTSAC	4 cut(s)	728, 847, 1714, 2851
TspGWI	ACGGA	7 cut(s)	48, 108, 568, 782, 2643, 3012, 3101
TspRI	CASTG	4 cut(s)	660, 848, 1428, 1839
Tth111I	GACNNNGTC	1 cut(s)	2312
VpaK11BI	GGWCC	4 cut(s)	637, 1220, 1469, 2749
XagI	CCTNNNNNAGG	1 cut(s)	1268
XapI	RAATTY	4 cut(s)	529, 574, 1999, 2906
XceI	RCATGY	1 cut(s)	2385
XhoI	CTCGAG	1 cut(s)	1022
XmiI	GTMKAC	2 cut(s)	168, 414
XmnI	GAANNNNTTC	4 cut(s)	113, 2197, 2259, 2907
XspI	CTAG	9 cut(s)	878, 1043, 1559, 1622, 1899, 2102, 2214, 2703, 2783
Zsp2I	ATGCAT	1 cut(s)	2383

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.