Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004743

Physical Location & Seq

Genomic Coordinates Forward (+)

6896072 .. 6900354

Gene Structure 4283 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1040500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3162 bp

ATGGCGAACGAGAAAGTGAACAGGAAACTGAACGGAGAAGAAAATGAAGAAGAAGAGGAGCCGAAAATCCCCGTCTTCACAGTCCTCAAGAACGGAGCCATTCTCAAGAACATCTTTATCGTCAACAAATCCCCCCCGCCGCCGCACTCCAAACCCATCTCCGCAGTCCACCGGAAAACCCATGAAGAAATCTTGATCGTTGGCCGACACCCGGACTGCAACATCGTCTTGACTCACCCCAGCATCAGCAGATTCCACCTCCAAATCCTCTCCGACCCCTTTTCCCAAAAGCTCTCTCTCACCGATTTGTCTTCAGTACATGGGACTTGGGTTTCGGACAAGAAGATCGAGCCGGGAGTTCGAGTGGAGCTGAGCGAAGGGGACAGGCTCCAGCTTGGTGGTTCCAGCAGGGTCTACAGCCTGCACTGGATTCCTATGAGTCGGGCCTATGATTCTGAAACCCCTTTCGTGCCATTCATAGAGCACGAAGATGATGAAAGTGCAGAACAAGTAGTGGACCAGTGGGAGAATTCTTTGTCAGCTGAAAACAAAAAACCTGAATCTCCAGATTCAAATTCAGAGGGTATAGAATCGTCGTTCTCCGATGAAATTGTGGGAGTAATTGGGAAGATGGACGTCCCATCAGCACCTCCACTGCCGGAGAATGCCATTTACTCAGTCTGTGATCAAAGTGAAGAAGGTGGTGAGAACTTATCAAAAGGTAGCGGTGAAGAGAATGAAGTGTCAAGCTTCTGGGCATTTGGAACGGAGTCGGTGAACCTGTTTATGAATATGGAAGAATCTAATCCTAGTCGAAAGGAAAACCAGCACCTACAGCCTTACTGTGTCACAGAAGAGGTTTCTGAAAGAGAGAACCCAGAGAATTCCGTTTTTACTGCAGAGGAAGGAGAAGCTTATCATGCTGTGCATGTGCCTGAGGAAACTGAGAACCAAAGCCAATTGGGAAAAGACCATGGAAAGAATGATCTTTCGCCTCTTGTGACTGGGGAAATCCTCGAGGAGACTAAAATTCAGCTAATTGAGAAAGAAAATCTGACCCTGGAATCAAAACCAAACTTGCCAGTCAACCTGAATGCTGAACATTCTACCGGTGAAAAGGAAGATGAGGCATATCCTGCTACTCAAGTACCTGAGGAATTTGAGAGCCAAGGCCCTTCGAGAAAATATCAGGGACAGATTGATTGTATATGTCTTTCCTCTGGACCTCGGGTGATGGAGAACTCATCCTTGCGGACTGGGGAAGTCCTTGAGGAGGACAAAGATGAACAAATTCCAGAAGAAAACCTGACCCGAGGACCAAAACCGAACTTGCTGATTAGCCAAAAGTTTGAACATTTTGATGAAAAGGAAAAGGAAGCTTATGCTGCTGCTTCTGTACCTGAGAAACTTGAGAACCAAAGCCCATTGGGAAAAGATAATGGACAGACTGATATTTCATGTCTTTTTTCTGGACCTCTTGTGATGGAGAACTTATCATTGCCAATCGGGGAAGTCCTCGGGGAGACCAAAGATCAGAAAGTTGTAGAAGAAAGCCTGACCCCAGAACCAAGATCCAGCTTGCCCTTTAACCTAAATTTTGAACATTCTGATGAAAAAGAATGTCTAGTGGATGTGAGTTGTGGAGAATCGGAAAACAAAAGCGTGGCACCAGAAGATCATGAAAAGAGGGATACAAGCATTTCTTCTGCACTTCTTGTGACAGAATCTGGAAACTCATCTATGTCAGAGATAATGGATGACAAAGAGAGCCAGACCCCACAATCTCTCTTTACTGCAGCAGGACAGCCTGAATCGGAATTCTGTGAAAGTCCTCCACTGAGATCAGAGAATAAATCAAGTACGAGGATGGGAAGCGTTTGGGCAAGAAGAGGCAAACCTGCTAGTGCTGTAAAGCTTCAAACAGATAAGAGCAGAGGAAAATCTACAGAGGCTGGGTATGATGATGATATTGAAGAGGACGAGGAGATCTTTACTCCAGACAAGGAAAATTTCACCCCAAATACTATTCGACTGAGGTCTTTGAAAAAGAAGGGTACGATAAAAGTTAAGCATTCCAAATCAAGCACATCATCCTCGTCGAAGCTAAATCTAGTCTCCAATATCCATCAACAAGAGCTGATTGAATCCCCAGGAAAAGAGAACCAGATAATGAAGGAACTCCAGGAAACAAAATTAGTAGGAAATACTTCTGGAAATCAAGCAAGGGTGGAGAAAAAGTTGACAGTAACAAAATTAAGAAGAGAAAGGATTCCCTTTCAATCACTAAAAAGCTCCGGAGGCAAGAACATATCAGAAGACTCGGTCCCTAACACAGAGACAAAAAGCAGCATATCTTTCAGTTCTACTAAAAATAAGGAGGTCGCCAATGCACACTCTAATAAATCTGTTGGAGAAGGAAAGAGGAGCTGGACTATGGTTGCAGACGCTACTACTCTTCTAGACAAGGAATCAGGGAAGTCATTGCAGTTTCTACAAGGTCTTAAGGGGACTCAGTTAATCATTCCAAGAATGGTTATACAGGAACTGGATTGCTTGAAGCAACGTGGCAGCCTTTTCAGAAAGAAAACAGAGGCTGAATCGGTTCTGGAATGGATCGAAGAATGTATGGTTAAAACAAATTGGTGGATCCATGTCCAGAGCTCAATGGAAGATGGAAGACTGATTGCTCCAACCCCTCCTGTTTCTCCGCAGTCTCTATTTAGCGAGAAGAGTTGGTGCTTTCCTTCTGGGACAACGGGCTCATTGACTTTTTCGAGGTGTGGGAGCACAATGGACCTTGTATCACCCTCCCCAGAAGACCATATCCTAGATTGTGCTCTTTTACATAGAAGGATGAAGAGGAACGATGGACAACTTATCCTTCTCAGTAATGATGTTACCCTGAAGATCAAAGCCATGGCAGAGGGTTTGCTTTGTGAGACAGCTCAAGAATTTCGTGAGAGTCTGGTGAACCCACTTTCTGAGAGGTTTATGTGGCCGGACAGTTCTCCCTGTGGGCGTACATGGTCTTACTCCGGCAATGTAGCTTTGAGAGAAAAGTACAATAGCTGTGGCCCTCTGAAGAAGTTGTCAAATGGTGAAGGTGCAAAGGGCTTGAAGCTCATTCTGCACCACAATTCTCATTACGGGCAGATCCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004540 GO:0004857 GO:0004864 GO:0004865 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006401 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008995 GO:0009056 GO:0009057 GO:0009555 GO:0009892 GO:0009987 GO:0010563 GO:0010605 GO:0010921 GO:0010923 GO:0016070 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0019208 GO:0019212 GO:0019220 GO:0019222 GO:0019439 GO:0019888 GO:0030234 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032515 GO:0034641 GO:0034655 GO:0035303 GO:0035304 GO:0035305 GO:0035308 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043666 GO:0044092 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045936 GO:0046483 GO:0046700 GO:0048229 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0098772 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FHA PIN_4

Protein Analysis

1054

Amino Acids

117.06

Weight (kDa)

5.05

Isoelectric Point (pI)

58.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Yop-YscD_cpl	PF16697	61 - 133	3.4e-09	Inner membrane component of T3SS, cytoplasmic domain
FHA	PF00498	66 - 133	8.1e-17	FHA domain
PIN_4	PF13638	827 - 982	4.8e-19	PIN domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004743)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34355 AT1G34355 AT1G34355
fragaria_vesca	FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232 FvH4_2g12232
malus_domestica	MD05G1040500.v1.1 MD10G1046600.v1.1
prunus_persica	Prupe.8G054200_v2.0.a1
pyrus_communis	pycom05g03310 pycom10g03280 pycom10g03290
rosa_chinensis	RchiOBHm_Chr6g0272171
rosa_laevigata	RLG00000013694
rosa_multiflora	Rmu_sc0005748.1_g000012
rosa_roxburghii	Rroxscaffold_7G00196530
rosa_rugosa	Rorug06G0066700
rosa_samantha	Rh6AG183500 Rh6BG183500 Rh6BG183600 Rh6CG184900 Rh6DG176600
rosa_wichuraiana	Rw6G015820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	639
AbsI	CCTCGAGG	1 cut(s)	1016
Acc36I	ACCTGC	1 cut(s)	1906
AccB1I	GGYRCC	1 cut(s)	1666
AccI	GTMKAC	1 cut(s)	414
AccIII	TCCGGA	1 cut(s)	2294
AciI	CCGC	7 cut(s)	137, 140, 143, 162, 726, 1252, 2708
AclWI	GGATC	5 cut(s)	1566, 2621, 2641, 2654, 3148
AcoI	YGGCCR	2 cut(s)	202, 2996
AcuI	CTGAAG	3 cut(s)	297, 2924, 3101
AcyI	GRCGYC	1 cut(s)	636
AfaI	GTAC	7 cut(s)	318, 1149, 1398, 1861, 2056, 3022, 3062
AfiI	CCNNNNNNNGG	2 cut(s)	211, 1273
AflII	CTTAAG	1 cut(s)	2501
AgeI	ACCGGT	1 cut(s)	1109
AjnI	CCWGG	3 cut(s)	1059, 2149, 2181
Alw21I	GWGCWC	4 cut(s)	486, 2663, 2789, 2839
Alw26I	GTCTC	6 cut(s)	1016, 1517, 2119, 2330, 2718, 2933
AlwI	GGATC	5 cut(s)	1566, 2621, 2641, 2654, 3148
AlwNI	CAGNNNCTG	4 cut(s)	1727, 1952, 2545, 2594
Ama87I	CYCGRG	4 cut(s)	1016, 1227, 1311, 1517
Aor13HI	TCCGGA	1 cut(s)	2294
AoxI	GGCC	5 cut(s)	202, 444, 1171, 2996, 3073
ApeKI	GCWGC	5 cut(s)	1385, 1388, 1796, 2346, 2568
ArsI	GACNNNNNNTTYG	2 cut(s)	316, 348
AsiGI	ACCGGT	1 cut(s)	1109
Asp700I	GAANNNNTTC	4 cut(s)	1290, 2206, 2268, 2601
AspS9I	GGNCC	9 cut(s)	444, 517, 1172, 1223, 1316, 1472, 2323, 2794, 3074
AsuC2I	CCSGG	2 cut(s)	212, 354
AvaI	CYCGRG	4 cut(s)	1016, 1227, 1311, 1517
AvaII	GGWCC	6 cut(s)	517, 1223, 1316, 1472, 2323, 2794
AxyI	CCTNAGG	2 cut(s)	936, 1152
BaeI	ACNNNNGTAYC	2 cut(s)	1131, 1164
BamHI	GGATCC	1 cut(s)	2646
BanI	GGYRCC	1 cut(s)	1666
BanII	GRGCYC	2 cut(s)	2663, 2762
BbsI	GAAGAC	5 cut(s)	67, 303, 2322, 2683, 2823
Bbv12I	GWGCWC	4 cut(s)	486, 2663, 2789, 2839
BbvI	GCAGC	5 cut(s)	1372, 1375, 1808, 2358, 2580
BccI	CCATC	9 cut(s)	164, 625, 649, 1228, 1477, 1861, 2133, 2666, 2861
BciT130I	CCWGG	3 cut(s)	1061, 2151, 2183
BciVI	GTATCC	1 cut(s)	1684
BclI	TGATCA	1 cut(s)	685
BcnI	CCSGG	2 cut(s)	212, 354
BcoDI	GTCTC	6 cut(s)	1016, 1517, 2119, 2330, 2718, 2933
BfaI	CTAG	6 cut(s)	810, 1625, 1902, 2111, 2459, 2828
BfmI	CTRYAG	5 cut(s)	415, 833, 897, 1794, 1944
BfrI	CTTAAG	1 cut(s)	2501
BfuAI	ACCTGC	1 cut(s)	1906
BfuI	GTATCC	1 cut(s)	1684
BglII	AGATCT	1 cut(s)	1986
BisI	GCNGC	7 cut(s)	140, 143, 1386, 1389, 1797, 2347, 2569
BlpI	GCTNAGC	1 cut(s)	371
BlsI	GCNGC	7 cut(s)	141, 144, 1387, 1390, 1798, 2348, 2570
Bme1390I	CCNGG	5 cut(s)	212, 354, 1061, 2151, 2183
Bme18I	GGWCC	6 cut(s)	517, 1223, 1316, 1472, 2323, 2794
BmeT110I	CYCGRG	4 cut(s)	1016, 1227, 1311, 1517
BmgT120I	GGNCC	9 cut(s)	444, 517, 1172, 1223, 1316, 1472, 2323, 2794, 3074
BmiI	GGNNCC	7 cut(s)	60, 97, 389, 403, 1668, 2325, 2648
BmrFI	CCNGG	5 cut(s)	212, 354, 1061, 2151, 2183
BmrI	ACTGGG	2 cut(s)	1014, 1266
BmsI	GCATC	1 cut(s)	252
BmuI	ACTGGG	2 cut(s)	1014, 1266
BoxI	GACNNNNGTC	1 cut(s)	2035
BpiI	GAAGAC	5 cut(s)	67, 303, 2322, 2683, 2823
BplI	GAGNNNNNCTC	2 cut(s)	87, 119
BpmI	CTGGAG	4 cut(s)	374, 549, 1980, 2165
Bpu1102I	GCTNAGC	1 cut(s)	371
BpuEI	CTTGAG	6 cut(s)	71, 89, 1128, 1289, 1430, 2931
BpuMI	CCSGG	2 cut(s)	212, 354
BsaHI	GRCGYC	1 cut(s)	636
BsaI	GGTCTC	1 cut(s)	1517
BsaJI	CCNNGG	8 cut(s)	973, 1059, 1168, 1226, 1312, 1516, 2149, 2916
BsaWI	WCCGGW	3 cut(s)	171, 1109, 2294
BsaXI	ACNNNNNCTCC	4 cut(s)	348, 378, 2222, 2252
Bsc4I	CCNNNNNNNGG	2 cut(s)	211, 1273
Bse118I	RCCGGY	1 cut(s)	1109
Bse1I	ACTGG	6 cut(s)	431, 520, 1009, 1082, 1261, 2550
Bse21I	CCTNAGG	2 cut(s)	936, 1152
Bse3DI	GCAATG	3 cut(s)	1496, 2480, 3046
BseAI	TCCGGA	1 cut(s)	2294
BseBI	CCWGG	3 cut(s)	1061, 2151, 2183
BseDI	CCNNGG	8 cut(s)	973, 1059, 1168, 1226, 1312, 1516, 2149, 2916
BseGI	GGATG	6 cut(s)	1244, 1636, 1762, 1872, 2090, 2859
BseLI	CCNNNNNNNGG	2 cut(s)	211, 1273
BseMI	GCAATG	3 cut(s)	1496, 2480, 3046
BseNI	ACTGG	6 cut(s)	431, 520, 1009, 1082, 1261, 2550
BseRI	GAGGAG	5 cut(s)	71, 1034, 1286, 1997, 2437
BseXI	GCAGC	5 cut(s)	1372, 1375, 1808, 2358, 2580
BseYI	CCCAGC	2 cut(s)	239, 1952
BsgI	GTGCAG	4 cut(s)	407, 522, 1692, 3113
BshFI	GGCC	5 cut(s)	204, 446, 1173, 2998, 3075
BshNI	GGYRCC	1 cut(s)	1666
BshTI	ACCGGT	1 cut(s)	1109
BsiHKAI	GWGCWC	4 cut(s)	486, 2663, 2789, 2839
BsiHKCI	CYCGRG	4 cut(s)	1016, 1227, 1311, 1517
BsiSI	CCGG	8 cut(s)	172, 212, 353, 659, 1110, 2295, 2999, 3036
BslFI	GGGAC	7 cut(s)	337, 395, 623, 1206, 2309, 2521, 2764
BslI	CCNNNNNNNGG	2 cut(s)	211, 1273
BsmAI	GTCTC	6 cut(s)	1016, 1517, 2119, 2330, 2718, 2933
BsmFI	GGGAC	7 cut(s)	337, 395, 623, 1206, 2309, 2521, 2764
BsmI	GAATGC	3 cut(s)	670, 1099, 2071
BsnI	GGCC	5 cut(s)	204, 446, 1173, 2998, 3075
Bso31I	GGTCTC	1 cut(s)	1517
BsoBI	CYCGRG	4 cut(s)	1016, 1227, 1311, 1517
Bsp1286I	GDGCHC	5 cut(s)	486, 2663, 2762, 2789, 2839
Bsp13I	TCCGGA	1 cut(s)	2294
Bsp1720I	GCTNAGC	1 cut(s)	371
Bsp19I	CCATGG	2 cut(s)	973, 2916
BspACI	CCGC	7 cut(s)	137, 140, 143, 162, 726, 1252, 2708
BspANI	GGCC	5 cut(s)	204, 446, 1173, 2998, 3075
BspEI	TCCGGA	1 cut(s)	2294
BspHI	TCATGA	1 cut(s)	1678
BspLI	GGNNCC	7 cut(s)	60, 97, 389, 403, 1668, 2325, 2648
BspMAI	CTGCAG	2 cut(s)	901, 1798
BspMI	ACCTGC	1 cut(s)	1906
BspPI	GGATC	5 cut(s)	1566, 2621, 2641, 2654, 3148
BspT107I	GGYRCC	1 cut(s)	1666
BspTI	CTTAAG	1 cut(s)	2501
BspTNI	GGTCTC	1 cut(s)	1517
BsrDI	GCAATG	3 cut(s)	1496, 2480, 3046
BsrFI	RCCGGY	1 cut(s)	1109
BsrI	ACTGG	6 cut(s)	431, 520, 1009, 1082, 1261, 2550
BssAI	RCCGGY	1 cut(s)	1109
BssECI	CCNNGG	8 cut(s)	973, 1059, 1168, 1226, 1312, 1516, 2149, 2916
BssNI	GRCGYC	1 cut(s)	636
BssT1I	CCWWGG	3 cut(s)	973, 1168, 2916
Bst2UI	CCWGG	3 cut(s)	1061, 2151, 2183
Bst4CI	ACNGT	4 cut(s)	82, 845, 2245, 3005
Bst6I	CTCTTC	9 cut(s)	48, 726, 849, 1882, 1968, 2254, 2460, 2723, 2852
BstACI	GRCGYC	1 cut(s)	636
BstAFI	CTTAAG	1 cut(s)	2501
BstAPI	GCANNNNNTGC	1 cut(s)	1136
BstC8I	GCNNGC	2 cut(s)	422, 1580
BstDSI	CCRYGG	2 cut(s)	973, 2916
BstENI	CCTNNNNNAGG	1 cut(s)	1271
BstF5I	GGATG	6 cut(s)	1244, 1636, 1762, 1872, 2090, 2859
BstMAI	GTCTC	6 cut(s)	1016, 1517, 2119, 2330, 2718, 2933
BstMWI	GCNNNNNNNGC	6 cut(s)	835, 920, 1136, 1385, 2298, 3127
BstNI	CCWGG	3 cut(s)	1061, 2151, 2183
BstNSI	RCATGY	1 cut(s)	932
BstPAI	GACNNNNGTC	1 cut(s)	2035
BstSCI	CCNGG	5 cut(s)	210, 352, 1059, 2149, 2181
BstSFI	CTRYAG	5 cut(s)	415, 833, 897, 1794, 1944
BstV1I	GCAGC	5 cut(s)	1372, 1375, 1808, 2358, 2580
BstV2I	GAAGAC	5 cut(s)	67, 303, 2322, 2683, 2823
BstX2I	RGATCY	4 cut(s)	1571, 1986, 2646, 3153
BstXI	CCANNNNNNTGG	1 cut(s)	398
BstYI	RGATCY	4 cut(s)	1571, 1986, 2646, 3153
Bsu36I	CCTNAGG	2 cut(s)	936, 1152
BsuI	GTATCC	1 cut(s)	1684
BsuRI	GGCC	5 cut(s)	204, 446, 1173, 2998, 3075
BtgI	CCRYGG	2 cut(s)	973, 2916
BtsCI	GGATG	6 cut(s)	1244, 1636, 1762, 1872, 2090, 2859
BtsI	GCAGTG	1 cut(s)	653
BtsIMutI	CAGTG	4 cut(s)	424, 527, 653, 1835
BveI	ACCTGC	1 cut(s)	1906
Cac8I	GCNNGC	2 cut(s)	422, 1580
CaiI	CAGNNNCTG	4 cut(s)	1727, 1952, 2545, 2594
CciI	TCATGA	1 cut(s)	1678
Cfr10I	RCCGGY	1 cut(s)	1109
Cfr13I	GGNCC	9 cut(s)	444, 517, 1172, 1223, 1316, 1472, 2323, 2794, 3074
CseI	GACGC	1 cut(s)	2453
Csp6I	GTAC	7 cut(s)	317, 1148, 1397, 1860, 2055, 3021, 3061
CspAI	ACCGGT	1 cut(s)	1109
CspCI	CAANNNNNGTGG	2 cut(s)	3052, 3087
CviQI	GTAC	7 cut(s)	317, 1148, 1397, 1860, 2055, 3021, 3061
EaeI	YGGCCR	2 cut(s)	202, 2996
Eam1104I	CTCTTC	9 cut(s)	48, 726, 849, 1882, 1968, 2254, 2460, 2723, 2852
EarI	CTCTTC	9 cut(s)	48, 726, 849, 1882, 1968, 2254, 2460, 2723, 2852
Ecl136II	GAGCTC	1 cut(s)	2661
Eco130I	CCWWGG	3 cut(s)	973, 1168, 2916
Eco24I	GRGCYC	2 cut(s)	2663, 2762
Eco31I	GGTCTC	1 cut(s)	1517
Eco47I	GGWCC	6 cut(s)	517, 1223, 1316, 1472, 2323, 2794
Eco53kI	GAGCTC	1 cut(s)	2661
Eco57I	CTGAAG	3 cut(s)	297, 2924, 3101
Eco81I	CCTNAGG	2 cut(s)	936, 1152
Eco88I	CYCGRG	4 cut(s)	1016, 1227, 1311, 1517
EcoICRI	GAGCTC	1 cut(s)	2661
EcoNI	CCTNNNNNAGG	1 cut(s)	1271
EcoO109I	RGGNCCY	1 cut(s)	1172
EcoRI	GAATTC	3 cut(s)	529, 883, 1817
EcoRII	CCWGG	3 cut(s)	1059, 2149, 2181
EcoT14I	CCWWGG	3 cut(s)	973, 1168, 2916
EcoT38I	GRGCYC	2 cut(s)	2663, 2762
ErhI	CCWWGG	3 cut(s)	973, 1168, 2916
FalI	AAGNNNNNCTT	4 cut(s)	98, 130, 1687, 1719
FaqI	GGGAC	7 cut(s)	337, 395, 623, 1206, 2309, 2521, 2764
FauI	CCCGC	1 cut(s)	144
FbaI	TGATCA	1 cut(s)	685
FblI	GTMKAC	1 cut(s)	414
Fnu4HI	GCNGC	7 cut(s)	140, 143, 1386, 1389, 1797, 2347, 2569
FokI	GGATG	6 cut(s)	1231, 1643, 1769, 1879, 2077, 2866
FriOI	GRGCYC	2 cut(s)	2663, 2762
Fsp4HI	GCNGC	7 cut(s)	140, 143, 1386, 1389, 1797, 2347, 2569
FspBI	CTAG	6 cut(s)	810, 1625, 1902, 2111, 2459, 2828
GluI	GCNGC	7 cut(s)	140, 143, 1386, 1389, 1797, 2347, 2569
GsaI	CCCAGC	2 cut(s)	243, 1956
GsuI	CTGGAG	4 cut(s)	374, 549, 1980, 2165
HaeIII	GGCC	5 cut(s)	204, 446, 1173, 2998, 3075
HapII	CCGG	8 cut(s)	172, 212, 353, 659, 1110, 2295, 2999, 3036
HgaI	GACGC	1 cut(s)	2453
Hin1I	GRCGYC	1 cut(s)	636
HincII	GTYRAC	3 cut(s)	124, 1087, 2241
HindII	GTYRAC	3 cut(s)	124, 1087, 2241
HindIII	AAGCTT	4 cut(s)	748, 912, 1377, 1913
HpaII	CCGG	8 cut(s)	172, 212, 353, 659, 1110, 2295, 2999, 3036
Hpy166II	GTNNAC	9 cut(s)	19, 124, 169, 415, 517, 778, 1087, 2241, 2971
Hpy8I	GTNNAC	9 cut(s)	19, 124, 169, 415, 517, 778, 1087, 2241, 2971
Hpy99I	CGWCG	2 cut(s)	598, 2101
HpyCH4III	ACNGT	4 cut(s)	82, 845, 2245, 3005
HpyCH4IV	ACGT	2 cut(s)	636, 2563
HpyF10VI	GCNNNNNNNGC	6 cut(s)	835, 920, 1136, 1385, 2298, 3127
HpySE526I	ACGT	2 cut(s)	636, 2563
Hsp92I	GRCGYC	1 cut(s)	636
Kpn2I	TCCGGA	1 cut(s)	2294
Ksp22I	TGATCA	1 cut(s)	685
LmnI	GCTCC	8 cut(s)	58, 95, 367, 393, 2297, 2424, 2692, 2784
Lsp1109I	GCAGC	5 cut(s)	1372, 1375, 1808, 2358, 2580
LweI	GCATC	1 cut(s)	252
MaeI	CTAG	6 cut(s)	810, 1625, 1902, 2111, 2459, 2828
MaeII	ACGT	2 cut(s)	636, 2563
MaeIII	GTNAC	5 cut(s)	847, 1000, 1717, 2245, 2896
MfeI	CAATTG	1 cut(s)	959
MflI	RGATCY	4 cut(s)	1571, 1986, 2646, 3153
MhlI	GDGCHC	5 cut(s)	486, 2663, 2762, 2789, 2839
MlyI	GAGTC	6 cut(s)	226, 448, 779, 2312, 2503, 2971
MmeI	TCCRAC	3 cut(s)	297, 2389, 2714
MroI	TCCGGA	1 cut(s)	2294
MroXI	GAANNNNTTC	4 cut(s)	1290, 2206, 2268, 2601
MseI	TTAA	6 cut(s)	1587, 2067, 2255, 2502, 2516, 2631
MslI	CAYNNNNRTG	3 cut(s)	489, 1359, 1608
MspA1I	CMGCKG	1 cut(s)	542
MspCI	CTTAAG	1 cut(s)	2501
MspI	CCGG	8 cut(s)	172, 212, 353, 659, 1110, 2295, 2999, 3036
MspR9I	CCNGG	5 cut(s)	212, 354, 1061, 2151, 2183
MunI	CAATTG	1 cut(s)	959
Mva1269I	GAATGC	3 cut(s)	670, 1099, 2071
MvaI	CCWGG	3 cut(s)	1061, 2151, 2183
MwoI	GCNNNNNNNGC	6 cut(s)	835, 920, 1136, 1385, 2298, 3127
NciI	CCSGG	2 cut(s)	212, 354
NcoI	CCATGG	2 cut(s)	973, 2916
NlaIV	GGNNCC	7 cut(s)	60, 97, 389, 403, 1668, 2325, 2648
NmuCI	GTSAC	3 cut(s)	847, 1000, 1717
NspI	RCATGY	1 cut(s)	932
PaeR7I	CTCGAG	1 cut(s)	1016
PagI	TCATGA	1 cut(s)	1678
PctI	GAATGC	3 cut(s)	670, 1099, 2071
PdmI	GAANNNNTTC	4 cut(s)	1290, 2206, 2268, 2601
PflFI	GACNNNGTC	1 cut(s)	2321
PfoI	TCCNGGA	1 cut(s)	2181
PinAI	ACCGGT	1 cut(s)	1109
PkrI	GCNGC	7 cut(s)	141, 144, 1387, 1390, 1798, 2348, 2570
PleI	GAGTC	6 cut(s)	226, 447, 778, 2312, 2503, 2970
PpsI	GAGTC	6 cut(s)	226, 447, 778, 2312, 2503, 2970
PshAI	GACNNNNGTC	1 cut(s)	2035
Psp124BI	GAGCTC	1 cut(s)	2663
Psp6I	CCWGG	3 cut(s)	1059, 2149, 2181
PspFI	CCCAGC	2 cut(s)	239, 1952
PspGI	CCWGG	3 cut(s)	1059, 2149, 2181
PspN4I	GGNNCC	7 cut(s)	60, 97, 389, 403, 1668, 2325, 2648
PspPI	GGNCC	9 cut(s)	444, 517, 1172, 1223, 1316, 1472, 2323, 2794, 3074
PspXI	VCTCGAGB	1 cut(s)	1016
PstI	CTGCAG	2 cut(s)	901, 1798
PstNI	CAGNNNCTG	4 cut(s)	1727, 1952, 2545, 2594
PsuI	RGATCY	4 cut(s)	1571, 1986, 2646, 3153
PsyI	GACNNNGTC	1 cut(s)	2321
PvuII	CAGCTG	1 cut(s)	542
RsaI	GTAC	7 cut(s)	318, 1149, 1398, 1861, 2056, 3022, 3062
RsaNI	GTAC	7 cut(s)	317, 1148, 1397, 1860, 2055, 3021, 3061
RseI	CAYNNNNRTG	3 cut(s)	489, 1359, 1608
SacI	GAGCTC	1 cut(s)	2663
SaqAI	TTAA	6 cut(s)	1587, 2067, 2255, 2502, 2516, 2631
SatI	GCNGC	7 cut(s)	140, 143, 1386, 1389, 1797, 2347, 2569
Sau96I	GGNCC	9 cut(s)	444, 517, 1172, 1223, 1316, 1472, 2323, 2794, 3074
SchI	GAGTC	6 cut(s)	226, 448, 779, 2312, 2503, 2971
ScrFI	CCNGG	5 cut(s)	212, 354, 1061, 2151, 2183
SduI	GDGCHC	5 cut(s)	486, 2663, 2762, 2789, 2839
SfaNI	GCATC	1 cut(s)	252
SfcI	CTRYAG	5 cut(s)	415, 833, 897, 1794, 1944
Sfr274I	CTCGAG	1 cut(s)	1016
SinI	GGWCC	6 cut(s)	517, 1223, 1316, 1472, 2323, 2794
SlaI	CTCGAG	1 cut(s)	1016
SmiMI	CAYNNNNRTG	3 cut(s)	489, 1359, 1608
SmlI	CTYRAG	8 cut(s)	86, 104, 1016, 1143, 1268, 1409, 2501, 2946
SmoI	CTYRAG	8 cut(s)	86, 104, 1016, 1143, 1268, 1409, 2501, 2946
SsiI	CCGC	7 cut(s)	137, 140, 143, 162, 726, 1252, 2708
SspMI	CTAG	6 cut(s)	810, 1625, 1902, 2111, 2459, 2828
SstI	GAGCTC	1 cut(s)	2663
StyD4I	CCNGG	5 cut(s)	210, 352, 1059, 2149, 2181
StyI	CCWWGG	3 cut(s)	973, 1168, 2916
TaaI	ACNGT	4 cut(s)	82, 845, 2245, 3005
TaiI	ACGT	2 cut(s)	639, 2566
TaqI	TCGA	9 cut(s)	348, 361, 814, 1017, 1178, 2029, 2099, 2616, 2774
TaqII	GACCGA	1 cut(s)	2311
TatI	WGTACW	2 cut(s)	316, 3060
TauI	GCSGC	2 cut(s)	142, 145
Tru1I	TTAA	6 cut(s)	1587, 2067, 2255, 2502, 2516, 2631
Tru9I	TTAA	6 cut(s)	1587, 2067, 2255, 2502, 2516, 2631
TscAI	CASTG	4 cut(s)	431, 527, 660, 1842
TseFI	GTSAC	3 cut(s)	847, 1000, 1717
TseI	GCWGC	5 cut(s)	1385, 1388, 1796, 2346, 2568
Tsp45I	GTSAC	3 cut(s)	847, 1000, 1717
TspGWI	ACGGA	5 cut(s)	48, 108, 782, 877, 3146
TspRI	CASTG	4 cut(s)	431, 527, 660, 1842
Tth111I	GACNNNGTC	1 cut(s)	2321
Vha464I	CTTAAG	1 cut(s)	2501
VpaK11BI	GGWCC	6 cut(s)	517, 1223, 1316, 1472, 2323, 2794
XagI	CCTNNNNNAGG	1 cut(s)	1271
XbaI	TCTAGA	1 cut(s)	2458
XceI	RCATGY	1 cut(s)	932
XhoI	CTCGAG	1 cut(s)	1016
XmiI	GTMKAC	1 cut(s)	414
XmnI	GAANNNNTTC	4 cut(s)	1290, 2206, 2268, 2601
XspI	CTAG	6 cut(s)	810, 1625, 1902, 2111, 2459, 2828
ZraI	GACGTC	1 cut(s)	637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.