Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0010378

Physical Location & Seq

Genomic Coordinates Forward (+)

12336540 .. 12340944

Gene Structure 4405 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g14020.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 708 bp

ATGGAAATGGATTCCAACAACCACAGCTTCTCCTCACCCACCTCCTCAAACGACATTGAGCAGATGCCGGAAACCCCCCACCGCGGCGGCTCCTACCACCGGCGCGCCCACTCCGACTTCTCCTCCTTCCGCCTCCCCAACTTCGACGACCTCCTCCTCTTCGACCCTTCCGACCTCGACCTCTCCTCCTTCCCCTCTCCCGTCCCGCCACGTGGCGCCACCTCCACCTCCTCCGCCGCCGTCCCCATGCCGATCGATGACTCCGACGACTCCTCCGCCTCCCGCCCCAGGCCCAGCTCCTCCGCCACCGCCGCTCATTTCCGCAGCCTCTCCGTCGACTCCGACTTCTTCGACGGCCTCGGATTCTCCGGAGACGCCGTCTCCGGGAAGGAAGTCGTTGGGGGTAAGAGGCCCGGCGCCGGCGGCCACAGGCATAGCAACTCCATGGACGGCTCCTTCGATTCTGAGCTCTCTCTCGATGGTCTCAAGAAGTCCATCACTCCTGATAAGCTCGCTGAGCTCTCTCTGATTGACCCCAAGAGAGCTAAAAGGATCTTAGCTAATAGGCAATCTGCGGCGAGGTCCAAGGAGAGGAAGGTGAGATATGCAAATGAGCTGGAGAGGAAGGTGCAGACGCTTCAGACAGAGGCTACCACTCTATCGGCACAGGTCACACTGTTACAGGTCTTGTATTTTCAGGCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006810 GO:0006811 GO:0006820 GO:0006913 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008272 GO:0009267 GO:0009292 GO:0009294 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009652 GO:0009889 GO:0009970 GO:0009987 GO:0009991 GO:0010468 GO:0010556 GO:0015698 GO:0019219 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031326 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043621 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045595 GO:0045596 GO:0046907 GO:0048519 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051019 GO:0051093 GO:0051169 GO:0051170 GO:0051171 GO:0051179 GO:0051234 GO:0051252 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071470 GO:0071496 GO:0072348 GO:0080090 GO:0097159 GO:0104004 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

bZIP_1

Protein Analysis

236

Amino Acids

25.46

Weight (kDa)

5.97

Isoelectric Point (pI)

56.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bZIP_2	PF07716	178 - 228	2.8e-07	Basic region leucine zipper
bZIP_1	PF00170	180 - 227	5.1e-09	bZIP transcription factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G43700
fragaria_vesca	FvH4_2g14020 FvH4_2g14020
malus_domestica	MD05G1022300.v1.1 MD10G1023800.v1.1
prunus_persica	Prupe.8G026300_v2.0.a1
pyrus_communis	pycom05g01310 pycom10g01570
rosa_chinensis	RchiOBHm_Chr6g0275921
rosa_laevigata	RLG00000013423
rosa_multiflora	Rmu_co8294175.1_g000001 Rmu_sc0002030.1_g000004
rosa_roxburghii	Rroxscaffold_7G00192770
rosa_rugosa	Rorug06G0097400 Rorug06G0097500 Rorug06G0097600
rosa_samantha	Rh6AG209000 Rh6BG213100 Rh6CG215800 Rh6DG205400
rosa_wichuraiana	Rw6G018220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	215, 416
AccBSI	CCGCTC	1 cut(s)	314
AccI	GTMKAC	1 cut(s)	336
AccII	CGCG	2 cut(s)	84, 105
AccIII	TCCGGA	1 cut(s)	368
AclWI	GGATC	1 cut(s)	560
AcoI	YGGCCR	1 cut(s)	424
AcuI	CTGAAG	1 cut(s)	623
AcvI	CACGTG	1 cut(s)	212
AcyI	GRCGYC	3 cut(s)	216, 375, 417
AfiI	CCNNNNNNNGG	7 cut(s)	83, 99, 212, 288, 384, 419, 591
AjnI	CCWGG	1 cut(s)	287
AluBI	AGCT	8 cut(s)	27, 297, 469, 511, 520, 545, 560, 616
AluI	AGCT	8 cut(s)	27, 297, 469, 511, 520, 545, 560, 616
Alw21I	GWGCWC	2 cut(s)	471, 522
Alw26I	GTCTC	3 cut(s)	366, 385, 488
AlwI	GGATC	1 cut(s)	560
Aor13HI	TCCGGA	1 cut(s)	368
AoxI	GGCC	4 cut(s)	290, 355, 410, 424
ApeKI	GCWGC	1 cut(s)	324
ArsI	GACNNNNNNTTYG	2 cut(s)	440, 472
AscI	GGCGCGCC	1 cut(s)	103
AspLEI	GCGC	4 cut(s)	105, 107, 218, 419
AspS9I	GGNCC	3 cut(s)	291, 411, 582
AsuC2I	CCSGG	2 cut(s)	385, 414
AsuHPI	GGTGA	2 cut(s)	27, 610
AvaII	GGWCC	1 cut(s)	582
BanI	GGYRCC	2 cut(s)	215, 416
BanII	GRGCYC	2 cut(s)	471, 522
BbrPI	CACGTG	1 cut(s)	212
Bbv12I	GWGCWC	2 cut(s)	471, 522
BbvI	GCAGC	1 cut(s)	336
BccI	CCATC	2 cut(s)	473, 503
BceAI	ACGGC	4 cut(s)	224, 362, 370, 466
BciT130I	CCWGG	1 cut(s)	289
BcnI	CCSGG	2 cut(s)	385, 414
BcoDI	GTCTC	3 cut(s)	366, 385, 488
BfoI	RGCGCY	2 cut(s)	219, 420
BisI	GCNGC	7 cut(s)	85, 88, 237, 312, 325, 424, 576
BlpI	GCTNAGC	1 cut(s)	516
BlsI	GCNGC	7 cut(s)	86, 89, 238, 313, 326, 425, 577
Bme1390I	CCNGG	3 cut(s)	289, 385, 414
Bme18I	GGWCC	1 cut(s)	582
BmgT120I	GGNCC	3 cut(s)	291, 411, 582
BmiI	GGNNCC	4 cut(s)	91, 217, 418, 454
BmrFI	CCNGG	3 cut(s)	289, 385, 414
BmsI	GCATC	1 cut(s)	54
BpmI	CTGGAG	1 cut(s)	638
Bpu1102I	GCTNAGC	1 cut(s)	516
BpuEI	CTTGAG	1 cut(s)	470
BpuMI	CCSGG	2 cut(s)	385, 414
Bsa29I	ATCGAT	1 cut(s)	255
BsaAI	YACGTR	1 cut(s)	212
BsaHI	GRCGYC	3 cut(s)	216, 375, 417
BsaI	GGTCTC	1 cut(s)	488
BsaJI	CCNNGG	5 cut(s)	82, 287, 358, 444, 585
BsaWI	WCCGGW	1 cut(s)	368
Bsc4I	CCNNNNNNNGG	7 cut(s)	83, 99, 212, 288, 384, 419, 591
Bse118I	RCCGGY	2 cut(s)	99, 419
BseAI	TCCGGA	1 cut(s)	368
BseBI	CCWGG	1 cut(s)	289
BseCI	ATCGAT	1 cut(s)	255
BseDI	CCNNGG	5 cut(s)	82, 287, 358, 444, 585
BseLI	CCNNNNNNNGG	7 cut(s)	83, 99, 212, 288, 384, 419, 591
BseMII	CTCAG	2 cut(s)	456, 507
BsePI	GCGCGC	1 cut(s)	103
BseRI	GAGGAG	9 cut(s)	22, 34, 112, 143, 146, 175, 220, 262, 289
BseXI	GCAGC	1 cut(s)	336
BseYI	CCCAGC	1 cut(s)	293
BsgI	GTGCAG	1 cut(s)	650
Bsh1236I	CGCG	2 cut(s)	84, 105
Bsh1285I	CGRYCG	1 cut(s)	255
BshFI	GGCC	4 cut(s)	292, 357, 412, 426
BshNI	GGYRCC	2 cut(s)	215, 416
BshVI	ATCGAT	1 cut(s)	255
BsiEI	CGRYCG	1 cut(s)	255
BsiHKAI	GWGCWC	2 cut(s)	471, 522
BsiSI	CCGG	6 cut(s)	68, 100, 369, 384, 414, 420
BslFI	GGGAC	2 cut(s)	188, 227
BslI	CCNNNNNNNGG	7 cut(s)	83, 99, 212, 288, 384, 419, 591
BsmAI	GTCTC	3 cut(s)	366, 385, 488
BsmBI	CGTCTC	2 cut(s)	366, 385
BsmFI	GGGAC	2 cut(s)	188, 227
BsnI	GGCC	4 cut(s)	292, 357, 412, 426
Bso31I	GGTCTC	1 cut(s)	488
Bsp1286I	GDGCHC	2 cut(s)	471, 522
Bsp13I	TCCGGA	1 cut(s)	368
Bsp143I	GATC	2 cut(s)	252, 552
Bsp1720I	GCTNAGC	1 cut(s)	516
Bsp19I	CCATGG	1 cut(s)	444
BspANI	GGCC	4 cut(s)	292, 357, 412, 426
BspCNI	CTCAG	2 cut(s)	457, 508
BspDI	ATCGAT	1 cut(s)	255
BspEI	TCCGGA	1 cut(s)	368
BspFNI	CGCG	2 cut(s)	84, 105
BspLI	GGNNCC	4 cut(s)	91, 217, 418, 454
BspPI	GGATC	1 cut(s)	560
BspT107I	GGYRCC	2 cut(s)	215, 416
BspTNI	GGTCTC	1 cut(s)	488
BsrBI	CCGCTC	1 cut(s)	314
BsrFI	RCCGGY	2 cut(s)	99, 419
BssAI	RCCGGY	2 cut(s)	99, 419
BssECI	CCNNGG	5 cut(s)	82, 287, 358, 444, 585
BssHII	GCGCGC	1 cut(s)	103
BssMI	GATC	2 cut(s)	252, 552
BssNI	GRCGYC	3 cut(s)	216, 375, 417
BssT1I	CCWWGG	2 cut(s)	444, 585
Bst2UI	CCWGG	1 cut(s)	289
Bst4CI	ACNGT	1 cut(s)	678
Bst6I	CTCTTC	1 cut(s)	164
BstACI	GRCGYC	3 cut(s)	216, 375, 417
BstBAI	YACGTR	1 cut(s)	212
BstC8I	GCNNGC	3 cut(s)	105, 421, 513
BstDEI	CTNAG	3 cut(s)	465, 516, 556
BstDSI	CCRYGG	2 cut(s)	82, 444
BstFNI	CGCG	2 cut(s)	84, 105
BstH2I	RGCGCY	2 cut(s)	219, 420
BstHHI	GCGC	4 cut(s)	105, 107, 218, 419
BstKTI	GATC	2 cut(s)	255, 555
BstMAI	GTCTC	3 cut(s)	366, 385, 488
BstMBI	GATC	2 cut(s)	252, 552
BstMCI	CGRYCG	1 cut(s)	255
BstMWI	GCNNNNNNNGC	3 cut(s)	311, 423, 517
BstNI	CCWGG	1 cut(s)	289
BstSCI	CCNGG	3 cut(s)	287, 383, 412
BstUI	CGCG	2 cut(s)	84, 105
BstV1I	GCAGC	1 cut(s)	336
BstX2I	RGATCY	1 cut(s)	552
BstYI	RGATCY	1 cut(s)	552
Bsu15I	ATCGAT	1 cut(s)	255
BsuRI	GGCC	4 cut(s)	292, 357, 412, 426
BsuTUI	ATCGAT	1 cut(s)	255
BtgI	CCRYGG	2 cut(s)	82, 444
BtsIMutI	CAGTG	1 cut(s)	674
Cac8I	GCNNGC	3 cut(s)	105, 421, 513
CfoI	GCGC	4 cut(s)	105, 107, 218, 419
Cfr10I	RCCGGY	2 cut(s)	99, 419
Cfr13I	GGNCC	3 cut(s)	291, 411, 582
Cfr42I	CCGCGG	1 cut(s)	85
ClaI	ATCGAT	1 cut(s)	255
CseI	GACGC	2 cut(s)	383, 643
CviAII	CATG	2 cut(s)	247, 445
DdeI	CTNAG	3 cut(s)	465, 516, 556
DinI	GGCGCC	2 cut(s)	217, 418
DpnI	GATC	2 cut(s)	254, 554
DpnII	GATC	2 cut(s)	252, 552
EaeI	YGGCCR	1 cut(s)	424
Eam1104I	CTCTTC	1 cut(s)	164
EarI	CTCTTC	1 cut(s)	164
EciI	GGCGGA	4 cut(s)	119, 223, 265, 292
Ecl136II	GAGCTC	2 cut(s)	469, 520
Eco130I	CCWWGG	2 cut(s)	444, 585
Eco24I	GRGCYC	2 cut(s)	471, 522
Eco31I	GGTCTC	1 cut(s)	488
Eco47I	GGWCC	1 cut(s)	582
Eco53kI	GAGCTC	2 cut(s)	469, 520
Eco57I	CTGAAG	1 cut(s)	623
Eco72I	CACGTG	1 cut(s)	212
EcoICRI	GAGCTC	2 cut(s)	469, 520
EcoRII	CCWGG	1 cut(s)	287
EcoT14I	CCWWGG	2 cut(s)	444, 585
EcoT38I	GRGCYC	2 cut(s)	471, 522
EgeI	GGCGCC	2 cut(s)	217, 418
EheI	GGCGCC	2 cut(s)	217, 418
ErhI	CCWWGG	2 cut(s)	444, 585
Esp3I	CGTCTC	2 cut(s)	366, 385
FaeI	CATG	2 cut(s)	250, 448
FaiI	YATR	4 cut(s)	248, 435, 446, 606
FaqI	GGGAC	2 cut(s)	188, 227
FatI	CATG	2 cut(s)	246, 444
FauI	CCCGC	2 cut(s)	213, 290
FblI	GTMKAC	1 cut(s)	336
Fnu4HI	GCNGC	7 cut(s)	85, 88, 237, 312, 325, 424, 576
FriOI	GRGCYC	2 cut(s)	471, 522
Fsp4HI	GCNGC	7 cut(s)	85, 88, 237, 312, 325, 424, 576
GlaI	GCGC	4 cut(s)	104, 106, 217, 418
GluI	GCNGC	7 cut(s)	85, 88, 237, 312, 325, 424, 576
GsaI	CCCAGC	1 cut(s)	297
GsuI	CTGGAG	1 cut(s)	638
HaeII	RGCGCY	2 cut(s)	219, 420
HaeIII	GGCC	4 cut(s)	292, 357, 412, 426
HapII	CCGG	6 cut(s)	68, 100, 369, 384, 414, 420
HgaI	GACGC	2 cut(s)	383, 643
HhaI	GCGC	4 cut(s)	105, 107, 218, 419
Hin1I	GRCGYC	3 cut(s)	216, 375, 417
Hin1II	CATG	2 cut(s)	250, 448
Hin6I	GCGC	4 cut(s)	103, 105, 216, 417
HinP1I	GCGC	4 cut(s)	103, 105, 216, 417
HincII	GTYRAC	1 cut(s)	337
HindII	GTYRAC	1 cut(s)	337
HinfI	GANTC	6 cut(s)	11, 260, 269, 338, 363, 461
HpaII	CCGG	6 cut(s)	68, 100, 369, 384, 414, 420
HphI	GGTGA	2 cut(s)	27, 610
Hpy166II	GTNNAC	1 cut(s)	337
Hpy188I	TCNGA	8 cut(s)	115, 172, 265, 343, 362, 466, 528, 642
Hpy188III	TCNNGA	5 cut(s)	369, 476, 487, 503, 705
Hpy8I	GTNNAC	1 cut(s)	337
Hpy99I	CGWCG	4 cut(s)	149, 269, 338, 356
HpyAV	CCTTC	7 cut(s)	136, 177, 199, 382, 466, 589, 619
HpyCH4III	ACNGT	1 cut(s)	678
HpyCH4IV	ACGT	1 cut(s)	211
HpyCH4V	TGCA	2 cut(s)	608, 631
HpyF10VI	GCNNNNNNNGC	3 cut(s)	311, 423, 517
HpyF3I	CTNAG	3 cut(s)	465, 516, 556
HpySE526I	ACGT	1 cut(s)	211
Hsp92I	GRCGYC	3 cut(s)	216, 375, 417
Hsp92II	CATG	2 cut(s)	250, 448
HspAI	GCGC	4 cut(s)	103, 105, 216, 417
KasI	GGCGCC	2 cut(s)	215, 416
Kpn2I	TCCGGA	1 cut(s)	368
KroI	GCCGGC	1 cut(s)	419
KroNI	GCCGGC	1 cut(s)	421
KspI	CCGCGG	1 cut(s)	85
Kzo9I	GATC	2 cut(s)	252, 552
LmnI	GCTCC	3 cut(s)	95, 302, 458
Lsp1109I	GCAGC	1 cut(s)	336
LweI	GCATC	1 cut(s)	54
MaeII	ACGT	1 cut(s)	211
MaeIII	GTNAC	2 cut(s)	670, 678
MalI	GATC	2 cut(s)	254, 554
MbiI	CCGCTC	1 cut(s)	314
MboI	GATC	2 cut(s)	252, 552
MboII	GAAGA	2 cut(s)	151, 340
MflI	RGATCY	1 cut(s)	552
MhlI	GDGCHC	2 cut(s)	471, 522
Mly113I	GGCGCC	2 cut(s)	216, 417
MlyI	GAGTC	3 cut(s)	254, 263, 332
MmeI	TCCRAC	5 cut(s)	39, 138, 195, 288, 366
MreI	CGCCGGCG	1 cut(s)	419
MroI	TCCGGA	1 cut(s)	368
MroNI	GCCGGC	1 cut(s)	419
MspA1I	CMGCKG	1 cut(s)	84
MspI	CCGG	6 cut(s)	68, 100, 369, 384, 414, 420
MspR9I	CCNGG	3 cut(s)	289, 385, 414
MvaI	CCWGG	1 cut(s)	289
MvnI	CGCG	2 cut(s)	84, 105
MwoI	GCNNNNNNNGC	3 cut(s)	311, 423, 517
NaeI	GCCGGC	1 cut(s)	421
NarI	GGCGCC	2 cut(s)	216, 417
NciI	CCSGG	2 cut(s)	385, 414
NcoI	CCATGG	1 cut(s)	444
NdeII	GATC	2 cut(s)	252, 552
NgoMIV	GCCGGC	1 cut(s)	419
NlaIII	CATG	2 cut(s)	250, 448
NlaIV	GGNNCC	4 cut(s)	91, 217, 418, 454
NmuCI	GTSAC	1 cut(s)	670
PalAI	GGCGCGCC	1 cut(s)	103
PauI	GCGCGC	1 cut(s)	103
PcsI	WCGNNNNNNNCGW	3 cut(s)	168, 261, 456
PdiI	GCCGGC	1 cut(s)	421
PfeI	GAWTC	3 cut(s)	11, 363, 461
PflFI	GACNNNGTC	1 cut(s)	377
PfoI	TCCNGGA	1 cut(s)	383
PkrI	GCNGC	7 cut(s)	86, 89, 238, 313, 326, 425, 577
Ple19I	CGATCG	1 cut(s)	255
PleI	GAGTC	3 cut(s)	254, 263, 332
PluTI	GGCGCC	2 cut(s)	219, 420
PmaCI	CACGTG	1 cut(s)	212
PmlI	CACGTG	1 cut(s)	212
PpsI	GAGTC	3 cut(s)	254, 263, 332
Ppu21I	YACGTR	1 cut(s)	212
Psp124BI	GAGCTC	2 cut(s)	471, 522
Psp6I	CCWGG	1 cut(s)	287
PspCI	CACGTG	1 cut(s)	212
PspFI	CCCAGC	1 cut(s)	293
PspGI	CCWGG	1 cut(s)	287
PspN4I	GGNNCC	4 cut(s)	91, 217, 418, 454
PspPI	GGNCC	3 cut(s)	291, 411, 582
PsuI	RGATCY	1 cut(s)	552
PsyI	GACNNNGTC	1 cut(s)	377
PteI	GCGCGC	1 cut(s)	103
PvuI	CGATCG	1 cut(s)	255
SacI	GAGCTC	2 cut(s)	471, 522
SacII	CCGCGG	1 cut(s)	85
SalI	GTCGAC	1 cut(s)	335
SatI	GCNGC	7 cut(s)	85, 88, 237, 312, 325, 424, 576
Sau3AI	GATC	2 cut(s)	252, 552
Sau96I	GGNCC	3 cut(s)	291, 411, 582
SchI	GAGTC	3 cut(s)	254, 263, 332
ScrFI	CCNGG	3 cut(s)	289, 385, 414
SduI	GDGCHC	2 cut(s)	471, 522
SfaNI	GCATC	1 cut(s)	54
SfoI	GGCGCC	2 cut(s)	217, 418
Sfr303I	CCGCGG	1 cut(s)	85
SgrAI	CRCCGGYG	2 cut(s)	99, 419
SgrBI	CCGCGG	1 cut(s)	85
SgsI	GGCGCGCC	1 cut(s)	103
SinI	GGWCC	1 cut(s)	582
SmlI	CTYRAG	1 cut(s)	485
SmoI	CTYRAG	1 cut(s)	485
SspDI	GGCGCC	2 cut(s)	215, 416
SstI	GAGCTC	2 cut(s)	471, 522
StyD4I	CCNGG	3 cut(s)	287, 383, 412
StyI	CCWWGG	2 cut(s)	444, 585
TaaI	ACNGT	1 cut(s)	678
TaiI	ACGT	1 cut(s)	214
TaqI	TCGA	8 cut(s)	144, 162, 177, 255, 336, 351, 459, 477
TauI	GCSGC	6 cut(s)	87, 90, 239, 314, 426, 578
TfiI	GAWTC	3 cut(s)	11, 363, 461
TscAI	CASTG	1 cut(s)	681
TseFI	GTSAC	1 cut(s)	670
TseI	GCWGC	1 cut(s)	324
Tsp45I	GTSAC	1 cut(s)	670
TspGWI	ACGGA	1 cut(s)	322
TspRI	CASTG	1 cut(s)	681
Tth111I	GACNNNGTC	1 cut(s)	377
VpaK11BI	GGWCC	1 cut(s)	582
XmiI	GTMKAC	1 cut(s)	336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.