FvH4_2g15450

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002091
Physical Location & Seq
Reverse (-)
13593933 .. 13598459
4527 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15450.t2

Sequence Viewer

Length: 1581 bp
ATGGCTTTGATAGGAAAAGTGCACCCTGATTGCGTAAATGTTGCGAATCCATACCATGAATGCACTGAAAATTGCCTGAGAAAGATTGCAGAAGGGAAAGGCCGGAAGAACAAGAAGAAAACAGATAATGGCGGTGATATAAAAGATGGTGAGTTCAACAAAAAGATGGCTGGAGATAGAAGAGTGGATGCAACTAGGGCATCTAATCCTTACCATCAATCTGGTCAATCTTTTCCAAAAAGAACTGCTGGTGCTGAAGCTCGTGGGGATATAAAAGAATCAGGCTCCATACTTAATGTTCCTATTTTGGGTGGACGAAAAGAGAGATCCCGACCAAAGACTCCGCAAGATCATGACAACGTATCTGACCGAAGTGTAGTTTATCCTTATGATGCTCAATCATCTCCATCTACGGACAAGGTGAAAGTAGAGAATGGGTCTGTCCTTGATGATCCTATATTTGGAAAAAGAAAGCCAGTCTCCCAACCAAGGCCTGTTGAAGAACATGAGAATGGCTCTGCCCTTGATGATCCTATATTTGGCAGAAGAAAGCCAGCAACCCAGCCAAAGCCCCAAGAGCTTGTTAATGGATCCGGTCAAGGTGCTGTCAATCCTTCTGGTCCTCCCTTGTCTCCATCTAATGAGATTGTGAACATAGGGAATGGCTCCACCCTTGATGATCTCATATTCGGCCGCAAAAAGCAGGGATCTGAACCAAAACCTCAAGAAGAGCTTGAGAATGTACCTGCTGTGAAATTAGAGAATGGTGACTATAAGTCTTATTCTGGACCACTTGCTGAAGTAAAAGATCCTACTTTGAACAAGCAAATCCAGTCATCTGGGGTAGTGCCTACATCTGGAATTATCAAAATGACCCCCTCTCTGTCTCTCCCCATGCACAATGGTGTGGAAGGTGCCACTGACTCACCACCTGAAAATAGAAGCTTCAGTTTCTCAGGTACACCCCATGTTTTTGAAGAGAGTGATGATGACGATGCACTATCTATTAGTTCTGATGCCAGCGTTTCCGTGGGAAAGTACCGTGTGAAAGGAAGCTTATCTTCAATTCTACAGTCAGTCTTTGACAAGTACGGTGATATAGCAGCAAGCTGTCAACTGGAATCAATTGTCATTCGCTCTTATTACCTTGAGTGTGTCTGTTATGTAGTTCAAGAGTTGAAACGCACTTCAGTGAAGCAGCTGACAAGATCAAAAGTCAAAGAAATGTTGGCTATGCTCAAGGATGTAGAATCTTCAGGAATGGATGTTGCTTGGCTTCGTCTCATGCTCAATGAATGCGCAGAATCCATTGATATCATGAGTCAGCATCGAGCTTTCGAAGTAGCTAAGGCCAACTGTGATCGTGACATCGAATCAGCAAGGAAAGAATTAGAATCCCAAATGGAGGCTTTGGCTGTAAAAGAAAAGGAGGTTGCTGATGCCAAGAAGCAAGTTTCTGTAACCAGAGCCCATCTGAGGGAGCTTGAGCTCAAATCATCCAGTTTGAGTGAGGCTGTTAGTAAAATGAAGTCCAAGGCCGAAAATCTTCAAAGCAAACCATTGTTGGACAAGGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

527

Amino Acids

57.44

Weight (kDa)

6.79

Isoelectric Point (pI)

54.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 275 - 468 5.8e-64 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1300
Acc36I ACCTGC 1 cut(s) 754
AccB1I GGYRCC 1 cut(s) 914
AciI CCGC 3 cut(s) 132, 344, 694
AclWI GGATC 7 cut(s) 321, 446, 524, 585, 598, 715, 803
AcoI YGGCCR 1 cut(s) 691
AcuI CTGAAG 5 cut(s) 276, 819, 931, 1173, 1239
AfaI GTAC 4 cut(s) 744, 961, 1040, 1091
AfiI CCNNNNNNNGG 8 cut(s) 308, 461, 489, 539, 857, 1405, 1476, 1477
AgsI TTSAA 8 cut(s) 157, 500, 820, 977, 1065, 1172, 1180, 1550
AhdI GACNNNNNGTC 1 cut(s) 775
AleI CACNNNNGTG 2 cut(s) 903, 1190
Alw21I GWGCWC 2 cut(s) 24, 1491
Alw26I GTCTC 4 cut(s) 484, 636, 891, 1286
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 7 cut(s) 321, 446, 524, 585, 598, 715, 803
AoxI GGCC 5 cut(s) 100, 491, 691, 1350, 1536
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 1103, 1198
Asp700I GAANNNNTTC 1 cut(s) 1545
AspLEI GCGC 1 cut(s) 1301
AspS9I GGNCC 2 cut(s) 620, 788
AsuHPI GGTGA 6 cut(s) 146, 161, 433, 779, 918, 1106
AsuII TTCGAA 1 cut(s) 1338
AvaII GGWCC 2 cut(s) 620, 788
BaeGI GKGCMC 1 cut(s) 24
BamHI GGATCC 1 cut(s) 590
BanI GGYRCC 1 cut(s) 914
BanII GRGCYC 2 cut(s) 1471, 1491
BauI CACGAG 1 cut(s) 261
Bbv12I GWGCWC 2 cut(s) 24, 1491
BbvI GCAGC 2 cut(s) 1115, 1210
BccI CCATC 6 cut(s) 140, 160, 222, 415, 643, 1479
BcoDI GTCTC 4 cut(s) 484, 636, 891, 1286
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 1070
BfuAI ACCTGC 1 cut(s) 754
BisI GCNGC 3 cut(s) 694, 1104, 1199
BlsI GCNGC 3 cut(s) 695, 1105, 1200
Bme18I GGWCC 2 cut(s) 620, 788
BmeRI GACNNNNNGTC 1 cut(s) 775
BmgT120I GGNCC 2 cut(s) 620, 788
BmiI GGNNCC 4 cut(s) 286, 592, 667, 916
BmsI GCATC 7 cut(s) 178, 209, 382, 985, 1006, 1336, 1429
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpmI CTGGAG 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 1347
Bpu14I TTCGAA 1 cut(s) 1338
BpuEI CTTGAG 5 cut(s) 708, 755, 1169, 1223, 1505
BsaJI CCNNGG 3 cut(s) 488, 1029, 1533
BsaWI WCCGGW 1 cut(s) 593
Bsc4I CCNNNNNNNGG 8 cut(s) 308, 461, 489, 539, 857, 1405, 1476, 1477
Bse1I ACTGG 4 cut(s) 476, 832, 1122, 1500
BseDI CCNNGG 3 cut(s) 488, 1029, 1533
BseGI GGATG 4 cut(s) 193, 1249, 1270, 1496
BseLI CCNNNNNNNGG 8 cut(s) 308, 461, 489, 539, 857, 1405, 1476, 1477
BseMII CTCAG 3 cut(s) 68, 969, 1466
BseNI ACTGG 4 cut(s) 476, 832, 1122, 1500
BseSI GKGCMC 1 cut(s) 24
BseX3I CGGCCG 1 cut(s) 691
BseXI GCAGC 2 cut(s) 1115, 1210
BseYI CCCAGC 1 cut(s) 561
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 5 cut(s) 102, 493, 693, 1352, 1538
BshNI GGYRCC 1 cut(s) 914
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 2 cut(s) 24, 1491
BsiSI CCGG 2 cut(s) 103, 594
BslI CCNNNNNNNGG 8 cut(s) 308, 461, 489, 539, 857, 1405, 1476, 1477
BsmAI GTCTC 4 cut(s) 484, 636, 891, 1286
BsmBI CGTCTC 1 cut(s) 1286
BsmI GAATGC 2 cut(s) 65, 1301
BsnI GGCC 5 cut(s) 102, 493, 693, 1352, 1538
Bsp119I TTCGAA 1 cut(s) 1338
Bsp1286I GDGCHC 3 cut(s) 24, 1471, 1491
BspACI CCGC 3 cut(s) 132, 344, 694
BspANI GGCC 5 cut(s) 102, 493, 693, 1352, 1538
BspCNI CTCAG 3 cut(s) 69, 968, 1467
BspHI TCATGA 2 cut(s) 352, 1317
BspLI GGNNCC 4 cut(s) 286, 592, 667, 916
BspMI ACCTGC 1 cut(s) 754
BspPI GGATC 7 cut(s) 321, 446, 524, 585, 598, 715, 803
BspQI GCTCTTC 1 cut(s) 723
BspT104I TTCGAA 1 cut(s) 1338
BspT107I GGYRCC 1 cut(s) 914
BsrI ACTGG 4 cut(s) 476, 832, 1122, 1500
BssECI CCNNGG 3 cut(s) 488, 1029, 1533
BssSI CACGAG 1 cut(s) 261
BssT1I CCWWGG 2 cut(s) 488, 1533
Bst2BI CACGAG 1 cut(s) 261
Bst4CI ACNGT 4 cut(s) 1043, 1074, 1094, 1358
Bst6I CTCTTC 3 cut(s) 175, 723, 972
BstBI TTCGAA 1 cut(s) 1338
BstC8I GCNNGC 3 cut(s) 555, 1021, 1108
BstDEI CTNAG 4 cut(s) 77, 955, 1347, 1475
BstDSI CCRYGG 1 cut(s) 1029
BstF5I GGATG 4 cut(s) 193, 1249, 1270, 1496
BstHHI GCGC 1 cut(s) 1301
BstMAI GTCTC 4 cut(s) 484, 636, 891, 1286
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 2 cut(s) 197, 577
BstSFI CTRYAG 1 cut(s) 1070
BstSLI GKGCMC 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 1115, 1210
BstX2I RGATCY 4 cut(s) 326, 590, 707, 808
BstXI CCANNNNNNTGG 2 cut(s) 221, 839
BstYI RGATCY 4 cut(s) 326, 590, 707, 808
BstZI CGGCCG 1 cut(s) 691
BsuRI GGCC 5 cut(s) 102, 493, 693, 1352, 1538
BtgI CCRYGG 1 cut(s) 1029
BtsCI GGATG 4 cut(s) 193, 1249, 1270, 1496
BtsIMutI CAGTG 3 cut(s) 63, 918, 1197
BveI ACCTGC 1 cut(s) 754
Cac8I GCNNGC 3 cut(s) 555, 1021, 1108
CciI TCATGA 2 cut(s) 352, 1317
CfoI GCGC 1 cut(s) 1301
Cfr13I GGNCC 2 cut(s) 620, 788
Csp6I GTAC 4 cut(s) 743, 960, 1039, 1090
CviAII CATG 7 cut(s) 56, 353, 506, 895, 968, 1285, 1318
CviQI GTAC 4 cut(s) 743, 960, 1039, 1090
DdeI CTNAG 4 cut(s) 77, 955, 1347, 1475
DriI GACNNNNNGTC 1 cut(s) 775
EaeI YGGCCR 1 cut(s) 691
EagI CGGCCG 1 cut(s) 691
Eam1104I CTCTTC 3 cut(s) 175, 723, 972
Eam1105I GACNNNNNGTC 1 cut(s) 775
EarI CTCTTC 3 cut(s) 175, 723, 972
Ecl136II GAGCTC 1 cut(s) 1489
EclXI CGGCCG 1 cut(s) 691
Eco130I CCWWGG 2 cut(s) 488, 1533
Eco147I AGGCCT 1 cut(s) 493
Eco24I GRGCYC 2 cut(s) 1471, 1491
Eco32I GATATC 1 cut(s) 1315
Eco47I GGWCC 2 cut(s) 620, 788
Eco52I CGGCCG 1 cut(s) 691
Eco53kI GAGCTC 1 cut(s) 1489
Eco57I CTGAAG 5 cut(s) 276, 819, 931, 1173, 1239
EcoICRI GAGCTC 1 cut(s) 1489
EcoRV GATATC 1 cut(s) 1315
EcoT14I CCWWGG 2 cut(s) 488, 1533
EcoT38I GRGCYC 2 cut(s) 1471, 1491
ErhI CCWWGG 2 cut(s) 488, 1533
Esp3I CGTCTC 1 cut(s) 1286
FaeI CATG 7 cut(s) 59, 356, 509, 898, 971, 1288, 1321
FalI AAGNNNNNCTT 4 cut(s) 717, 749, 1045, 1077
FatI CATG 7 cut(s) 55, 352, 505, 894, 967, 1284, 1317
Fnu4HI GCNGC 3 cut(s) 694, 1104, 1199
FokI GGATG 4 cut(s) 200, 1256, 1277, 1483
FriOI GRGCYC 2 cut(s) 1471, 1491
Fsp4HI GCNGC 3 cut(s) 694, 1104, 1199
FspBI CTAG 1 cut(s) 195
FspI TGCGCA 1 cut(s) 1300
GlaI GCGC 1 cut(s) 1300
GluI GCNGC 3 cut(s) 694, 1104, 1199
GsaI CCCAGC 1 cut(s) 565
GsuI CTGGAG 1 cut(s) 192
HaeIII GGCC 5 cut(s) 102, 493, 693, 1352, 1538
HapII CCGG 2 cut(s) 103, 594
HhaI GCGC 1 cut(s) 1301
Hin1II CATG 7 cut(s) 59, 356, 509, 898, 971, 1288, 1321
Hin6I GCGC 1 cut(s) 1299
HinP1I GCGC 1 cut(s) 1299
HincII GTYRAC 1 cut(s) 1115
HindII GTYRAC 1 cut(s) 1115
HindIII AAGCTT 2 cut(s) 943, 1054
HpaII CCGG 2 cut(s) 103, 594
HphI GGTGA 6 cut(s) 146, 161, 433, 779, 918, 1106
Hpy166II GTNNAC 5 cut(s) 22, 314, 652, 962, 1115
Hpy188I TCNGA 4 cut(s) 367, 712, 1015, 1476
Hpy188III TCNNGA 9 cut(s) 330, 353, 725, 786, 858, 1172, 1257, 1318, 1364
Hpy8I GTNNAC 5 cut(s) 22, 314, 652, 962, 1115
HpyAV CCTTC 3 cut(s) 86, 624, 905
HpyCH4III ACNGT 4 cut(s) 1043, 1074, 1094, 1358
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 6 cut(s) 22, 63, 89, 191, 898, 998
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 577
HpyF3I CTNAG 4 cut(s) 77, 955, 1347, 1475
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 7 cut(s) 59, 356, 509, 898, 971, 1288, 1321
HspAI GCGC 1 cut(s) 1299
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 3 cut(s) 290, 671, 1480
Lsp1109I GCAGC 2 cut(s) 1115, 1210
LweI GCATC 7 cut(s) 178, 209, 382, 985, 1006, 1336, 1429
MaeI CTAG 1 cut(s) 195
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 3 cut(s) 767, 1364, 1459
MfeI CAATTG 1 cut(s) 1125
MflI RGATCY 4 cut(s) 326, 590, 707, 808
MhlI GDGCHC 3 cut(s) 24, 1471, 1491
MluCI AATT 6 cut(s) 70, 755, 861, 1065, 1125, 1388
MlyI GAGTC 3 cut(s) 334, 917, 1330
MmeI TCCRAC 1 cut(s) 1545
MnlI CCTC 7 cut(s) 633, 732, 889, 1399, 1423, 1470, 1504
MroXI GAANNNNTTC 1 cut(s) 1545
MseI TTAA 2 cut(s) 294, 585
MslI CAYNNNNRTG 3 cut(s) 510, 903, 1190
MspA1I CMGCKG 1 cut(s) 1201
MspI CCGG 2 cut(s) 103, 594
MunI CAATTG 1 cut(s) 1125
Mva1269I GAATGC 2 cut(s) 65, 1301
MwoI GCNNNNNNNGC 2 cut(s) 197, 577
NlaIII CATG 7 cut(s) 59, 356, 509, 898, 971, 1288, 1321
NlaIV GGNNCC 4 cut(s) 286, 592, 667, 916
NmuCI GTSAC 2 cut(s) 767, 1364
NsbI TGCGCA 1 cut(s) 1300
NspV TTCGAA 1 cut(s) 1338
OliI CACNNNNGTG 2 cut(s) 903, 1190
PagI TCATGA 2 cut(s) 352, 1317
PceI AGGCCT 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 2 cut(s) 65, 1301
PdmI GAANNNNTTC 1 cut(s) 1545
PfeI GAWTC 7 cut(s) 46, 278, 1121, 1250, 1304, 1373, 1394
PflFI GACNNNGTC 1 cut(s) 1571
PkrI GCNGC 3 cut(s) 695, 1105, 1200
PleI GAGTC 3 cut(s) 334, 917, 1329
PpsI GAGTC 3 cut(s) 334, 917, 1329
Psp124BI GAGCTC 1 cut(s) 1491
PspFI CCCAGC 1 cut(s) 561
PspN4I GGNNCC 4 cut(s) 286, 592, 667, 916
PspPI GGNCC 2 cut(s) 620, 788
PsuI RGATCY 4 cut(s) 326, 590, 707, 808
PsyI GACNNNGTC 1 cut(s) 1571
PvuII CAGCTG 1 cut(s) 1201
RsaI GTAC 4 cut(s) 744, 961, 1040, 1091
RsaNI GTAC 4 cut(s) 743, 960, 1039, 1090
RseI CAYNNNNRTG 3 cut(s) 510, 903, 1190
SacI GAGCTC 1 cut(s) 1491
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 2 cut(s) 294, 585
SatI GCNGC 3 cut(s) 694, 1104, 1199
Sau96I GGNCC 2 cut(s) 620, 788
SchI GAGTC 3 cut(s) 334, 917, 1330
SduI GDGCHC 3 cut(s) 24, 1471, 1491
SfaNI GCATC 7 cut(s) 178, 209, 382, 985, 1006, 1336, 1429
SfcI CTRYAG 1 cut(s) 1070
SfuI TTCGAA 1 cut(s) 1338
SinI GGWCC 2 cut(s) 620, 788
SmiMI CAYNNNNRTG 3 cut(s) 510, 903, 1190
SmlI CTYRAG 5 cut(s) 723, 734, 1148, 1238, 1484
SmoI CTYRAG 5 cut(s) 723, 734, 1148, 1238, 1484
Sse9I AATT 6 cut(s) 70, 755, 861, 1065, 1125, 1388
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 3 cut(s) 132, 344, 694
SspMI CTAG 1 cut(s) 195
SstI GAGCTC 1 cut(s) 1491
StuI AGGCCT 1 cut(s) 493
StyI CCWWGG 2 cut(s) 488, 1533
TaaI ACNGT 4 cut(s) 1043, 1074, 1094, 1358
TaiI ACGT 1 cut(s) 363
TaqI TCGA 3 cut(s) 1330, 1338, 1371
TaqII GACCGA 1 cut(s) 384
TasI AATT 6 cut(s) 70, 755, 861, 1065, 1125, 1388
TauI GCSGC 1 cut(s) 696
TfiI GAWTC 7 cut(s) 46, 278, 1121, 1250, 1304, 1373, 1394
Tru1I TTAA 2 cut(s) 294, 585
Tru9I TTAA 2 cut(s) 294, 585
TscAI CASTG 3 cut(s) 70, 925, 1197
TseFI GTSAC 2 cut(s) 767, 1364
TseI GCWGC 2 cut(s) 1103, 1198
Tsp45I GTSAC 2 cut(s) 767, 1364
TspDTI ATGAA 3 cut(s) 72, 1308, 1541
TspGWI ACGGA 2 cut(s) 428, 1018
TspRI CASTG 3 cut(s) 70, 925, 1197
Tth111I GACNNNGTC 1 cut(s) 1571
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 2 cut(s) 620, 788
XcmI CCANNNNNNNNNTGG 1 cut(s) 1027
XmnI GAANNNNTTC 1 cut(s) 1545
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.