Rroxscaffold_7G00190360

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002091
Physical Location & Seq
Forward (+)
30467468 .. 30471738
4271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190360.1

Sequence Viewer

Length: 1479 bp
ATGGCTTTGATAGGAAGAGTGCACCCTGATTGCGTAAACGTTGCGAATCCGTACCATGAATGCACTGAAAATTGCCTGAGAAAAATTGCAGAAGGGAAAGGCCGGAAGCATAAAAAGAAATCAGGCTCCATACTTGATGTTCCTATATTGGGTGGACGAAAAGACAGATCCCGACCAAAGACTCCTCAAGATCTTGACAATGTATCTGACCGAAGTGTGGTTTATCCTTATGATGCTCAATCATCTCCATCTAAGGACAAGGTGAAAGTAGAGAATGGGTCTGTCCTTGATGATCCTATATTTGGAAAAAGAAAGCCAGTCTCCCAAACAAGGTCTGCTGAAGAGCATGAAAATGGTCAAGGTGTTGATCCTTCATCATTTCCAGAAAAGCTGAAAATAGAGAACGGCTCTGCCCTTGATGATCCTATATTTGGCAGAAGAAAGCAGGTAGCCCAGCCAAAGCCCCATGAAGAGATTGACAATGGATCCGGTCAAGGTTCAGTTAATCCTTCTGGTTCTCCCATGTCTCCATCTAATGAAATTGTGAAAATAGGGAATGGCTCCACCCTTGATGATCTTATATTTGGCCGCAAAAAGCAGGGATCTGAACCAAAGCCTCAAGAAGAGATTGAGAATGTACCTGTTGTGAAATTAGATAATGATGAGTATAAGTCTTATTCTGGACCGCTTGCTCAAGTAAAAGATCCTTCTTTGAACAAGAAGCAAATCAGTTCCTCTGGGGCAGTGCCTACATCTGGAATTATCAAAATGACCCCCTCTCTCTCTCTCCCCACGCACAATAGTGGAGAAGGTCCCACTGCCACACCTTCTGAAAACAGAAGCTTCAGTTTCTCAGGTTCTCCCTATGTTTTGGAAGAGAGTGATGATGACGATGCACGATCTGTCAATTCTGATTCCAGTGTTTCTGTGGGAAAGTACCGTGTGAAAGGAAGCTTATGTTCCATTCTCCAGTCAATCTTTGACAAGTATGGTGATATAGCAGCAAGCTGTCAATTGGAATCAATTGTCATTCGCTCTTATTACCTCGAGTGTGTGTGTTATGTGGTTCAAGAGTTGAAACGCACTTCGGTGAAGCAGCTGACAAAGTCAAAAATCAAAGAAATGTCAGCTATGCTCAAGGATGTAGAATCTTCAGGAATGGATGTTGGTTGGCTTCGTCTCATGCTCAATGAATGTGCACAATCCATTGATCTCGTGAGTCAGCATCGAGCATTCGAACTAGAAAAGGCCAACTGTGATCGTGGCATTGAATCAATAAGGAAAGAACTAGAGTCCCAGATGGAGGCTTTGGCTCTGAAAGAAAAGGAGGTTGCTGATGCCAAGAAGCAAGTTTCTGAAACCAGAGCCCATCTGAGGGATCTTGAGCTCAAATCATCTATTTTGAGTGAGACTATTAGTAATCTGAAGTCCAAGGTTGAAAATTTTCAAAGCAAGTCATTGTTGGACAAGGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

492

Amino Acids

54.0

Weight (kDa)

6.51

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 291 - 489 3e-65 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 436
AciI CCGC 2 cut(s) 589, 686
AclI AACGTT 1 cut(s) 39
AclWI GGATC 9 cut(s) 162, 287, 362, 416, 480, 493, 610, 698, 1386
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 1 cut(s) 1441
AcuI CTGAAG 4 cut(s) 360, 829, 1137, 1445
AfaI GTAC 3 cut(s) 53, 639, 938
AfiI CCNNNNNNNGG 9 cut(s) 149, 217, 302, 330, 431, 755, 1303, 1374, 1375
AgsI TTSAA 6 cut(s) 715, 1070, 1078, 1271, 1439, 1448
AleI CACNNNNGTG 2 cut(s) 801, 1088
AluBI AGCT 7 cut(s) 391, 843, 954, 1008, 1099, 1130, 1387
AluI AGCT 7 cut(s) 391, 843, 954, 1008, 1099, 1130, 1387
Alw21I GWGCWC 3 cut(s) 24, 1201, 1389
Alw26I GTCTC 4 cut(s) 325, 531, 1184, 1403
Alw44I GTGCAC 2 cut(s) 20, 1197
AlwI GGATC 9 cut(s) 162, 287, 362, 416, 480, 493, 610, 698, 1386
Ama87I CYCGRG 1 cut(s) 1046
AoxI GGCC 3 cut(s) 100, 586, 1248
ApaLI GTGCAC 2 cut(s) 20, 1197
ApeKI GCWGC 2 cut(s) 1001, 1096
ApoI RAATTY 1 cut(s) 1441
Asp700I GAANNNNTTC 1 cut(s) 1443
AspS9I GGNCC 2 cut(s) 683, 812
AsuHPI GGTGA 3 cut(s) 274, 1004, 1102
AsuII TTCGAA 1 cut(s) 1236
AvaI CYCGRG 1 cut(s) 1046
AvaII GGWCC 2 cut(s) 683, 812
BaeGI GKGCMC 2 cut(s) 24, 1201
BamHI GGATCC 1 cut(s) 485
BanII GRGCYC 2 cut(s) 1369, 1389
BauI CACGAG 1 cut(s) 1214
Bbv12I GWGCWC 3 cut(s) 24, 1201, 1389
BbvI GCAGC 2 cut(s) 1013, 1108
BccI CCATC 4 cut(s) 256, 538, 1294, 1377
BceAI ACGGC 1 cut(s) 421
BcoDI GTCTC 4 cut(s) 325, 531, 1184, 1403
BfaI CTAG 2 cut(s) 1241, 1289
BfuAI ACCTGC 1 cut(s) 436
BglII AGATCT 1 cut(s) 190
BisI GCNGC 3 cut(s) 589, 1002, 1097
BlsI GCNGC 3 cut(s) 590, 1003, 1098
Bme18I GGWCC 2 cut(s) 683, 812
BmeT110I CYCGRG 1 cut(s) 1046
BmgT120I GGNCC 2 cut(s) 683, 812
BmiI GGNNCC 4 cut(s) 127, 487, 562, 814
BmsI GCATC 4 cut(s) 223, 883, 1234, 1327
BplI GAGNNNNNCTC 2 cut(s) 392, 424
BpmI CTGGAG 1 cut(s) 953
Bpu14I TTCGAA 1 cut(s) 1236
BpuEI CTTGAG 5 cut(s) 171, 603, 678, 1121, 1403
BsaJI CCNNGG 1 cut(s) 1431
BsaWI WCCGGW 1 cut(s) 488
Bsc4I CCNNNNNNNGG 9 cut(s) 149, 217, 302, 330, 431, 755, 1303, 1374, 1375
Bse1I ACTGG 3 cut(s) 317, 918, 970
BseDI CCNNGG 1 cut(s) 1431
BseGI GGATG 2 cut(s) 1147, 1168
BseLI CCNNNNNNNGG 9 cut(s) 149, 217, 302, 330, 431, 755, 1303, 1374, 1375
BseMII CTCAG 3 cut(s) 68, 867, 1364
BseNI ACTGG 3 cut(s) 317, 918, 970
BseRI GAGGAG 1 cut(s) 174
BseSI GKGCMC 2 cut(s) 24, 1201
BseXI GCAGC 2 cut(s) 1013, 1108
BseYI CCCAGC 1 cut(s) 453
BshFI GGCC 3 cut(s) 102, 588, 1250
BsiHKAI GWGCWC 3 cut(s) 24, 1201, 1389
BsiHKCI CYCGRG 1 cut(s) 1046
BsiSI CCGG 2 cut(s) 103, 489
BslFI GGGAC 2 cut(s) 798, 1279
BslI CCNNNNNNNGG 9 cut(s) 149, 217, 302, 330, 431, 755, 1303, 1374, 1375
BsmAI GTCTC 4 cut(s) 325, 531, 1184, 1403
BsmBI CGTCTC 1 cut(s) 1184
BsmFI GGGAC 2 cut(s) 798, 1279
BsmI GAATGC 2 cut(s) 65, 1232
BsnI GGCC 3 cut(s) 102, 588, 1250
BsoBI CYCGRG 1 cut(s) 1046
Bsp119I TTCGAA 1 cut(s) 1236
Bsp1286I GDGCHC 4 cut(s) 24, 1201, 1369, 1389
BspACI CCGC 2 cut(s) 589, 686
BspANI GGCC 3 cut(s) 102, 588, 1250
BspCNI CTCAG 3 cut(s) 69, 866, 1365
BspLI GGNNCC 4 cut(s) 127, 487, 562, 814
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 9 cut(s) 162, 287, 362, 416, 480, 493, 610, 698, 1386
BspQI GCTCTTC 1 cut(s) 336
BspT104I TTCGAA 1 cut(s) 1236
BsrI ACTGG 3 cut(s) 317, 918, 970
BssECI CCNNGG 1 cut(s) 1431
BssSI CACGAG 1 cut(s) 1214
BssT1I CCWWGG 1 cut(s) 1431
Bst2BI CACGAG 1 cut(s) 1214
Bst4CI ACNGT 2 cut(s) 941, 1256
Bst6I CTCTTC 5 cut(s) 10, 336, 465, 618, 870
BstBI TTCGAA 1 cut(s) 1236
BstC8I GCNNGC 2 cut(s) 690, 1006
BstDEI CTNAG 4 cut(s) 77, 252, 853, 1373
BstF5I GGATG 2 cut(s) 1147, 1168
BstMAI GTCTC 4 cut(s) 325, 531, 1184, 1403
BstSLI GKGCMC 2 cut(s) 24, 1201
BstV1I GCAGC 2 cut(s) 1013, 1108
BstX2I RGATCY 6 cut(s) 167, 190, 485, 602, 703, 1378
BstYI RGATCY 6 cut(s) 167, 190, 485, 602, 703, 1378
BsuRI GGCC 3 cut(s) 102, 588, 1250
BtsCI GGATG 2 cut(s) 1147, 1168
BtsI GCAGTG 2 cut(s) 750, 816
BtsIMutI CAGTG 4 cut(s) 63, 750, 816, 925
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 2 cut(s) 690, 1006
Cfr13I GGNCC 2 cut(s) 683, 812
Csp6I GTAC 3 cut(s) 52, 638, 937
CviAII CATG 5 cut(s) 56, 347, 467, 523, 1183
CviQI GTAC 3 cut(s) 52, 638, 937
DdeI CTNAG 4 cut(s) 77, 252, 853, 1373
EaeI YGGCCR 1 cut(s) 586
Eam1104I CTCTTC 5 cut(s) 10, 336, 465, 618, 870
EarI CTCTTC 5 cut(s) 10, 336, 465, 618, 870
Ecl136II GAGCTC 1 cut(s) 1387
Eco130I CCWWGG 1 cut(s) 1431
Eco24I GRGCYC 2 cut(s) 1369, 1389
Eco47I GGWCC 2 cut(s) 683, 812
Eco53kI GAGCTC 1 cut(s) 1387
Eco57I CTGAAG 4 cut(s) 360, 829, 1137, 1445
Eco88I CYCGRG 1 cut(s) 1046
EcoICRI GAGCTC 1 cut(s) 1387
EcoO109I RGGNCCY 1 cut(s) 812
EcoT14I CCWWGG 1 cut(s) 1431
EcoT38I GRGCYC 2 cut(s) 1369, 1389
ErhI CCWWGG 1 cut(s) 1431
Esp3I CGTCTC 1 cut(s) 1184
FaeI CATG 5 cut(s) 59, 350, 470, 526, 1186
FaqI GGGAC 2 cut(s) 798, 1279
FatI CATG 5 cut(s) 55, 346, 466, 522, 1182
Fnu4HI GCNGC 3 cut(s) 589, 1002, 1097
FokI GGATG 2 cut(s) 1154, 1175
FriOI GRGCYC 2 cut(s) 1369, 1389
Fsp4HI GCNGC 3 cut(s) 589, 1002, 1097
FspBI CTAG 2 cut(s) 1241, 1289
GluI GCNGC 3 cut(s) 589, 1002, 1097
GsaI CCCAGC 1 cut(s) 457
GsuI CTGGAG 1 cut(s) 953
HaeIII GGCC 3 cut(s) 102, 588, 1250
HapII CCGG 2 cut(s) 103, 489
Hin1II CATG 5 cut(s) 59, 350, 470, 526, 1186
HindIII AAGCTT 2 cut(s) 841, 952
HinfI GANTC 8 cut(s) 46, 181, 914, 1019, 1148, 1219, 1271, 1292
HpaII CCGG 2 cut(s) 103, 489
HphI GGTGA 3 cut(s) 274, 1004, 1102
Hpy166II GTNNAC 4 cut(s) 22, 37, 155, 1199
Hpy188I TCNGA 8 cut(s) 208, 607, 832, 913, 1317, 1357, 1374, 1425
Hpy8I GTNNAC 4 cut(s) 22, 37, 155, 1199
HpyAV CCTTC 6 cut(s) 86, 381, 519, 717, 803, 837
HpyCH4III ACNGT 2 cut(s) 941, 1256
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 5 cut(s) 22, 63, 89, 896, 1199
HpyF3I CTNAG 4 cut(s) 77, 252, 853, 1373
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 5 cut(s) 59, 350, 470, 526, 1186
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 2 cut(s) 131, 566
Lsp1109I GCAGC 2 cut(s) 1013, 1108
LweI GCATC 4 cut(s) 223, 883, 1234, 1327
MaeI CTAG 2 cut(s) 1241, 1289
MaeII ACGT 1 cut(s) 39
MboII GAAGA 7 cut(s) 27, 353, 450, 482, 635, 887, 1143
MfeI CAATTG 2 cut(s) 1013, 1023
MflI RGATCY 6 cut(s) 167, 190, 485, 602, 703, 1378
MhlI GDGCHC 4 cut(s) 24, 1201, 1369, 1389
MluCI AATT 9 cut(s) 70, 84, 540, 650, 759, 907, 1013, 1023, 1441
MlyI GAGTC 3 cut(s) 175, 1228, 1301
MmeI TCCRAC 1 cut(s) 1443
MnlI CCTC 8 cut(s) 195, 627, 745, 787, 1055, 1297, 1321, 1368
MroXI GAANNNNTTC 1 cut(s) 1443
MseI TTAA 1 cut(s) 504
MslI CAYNNNNRTG 3 cut(s) 351, 801, 1088
MspA1I CMGCKG 1 cut(s) 1099
MspI CCGG 2 cut(s) 103, 489
MunI CAATTG 2 cut(s) 1013, 1023
Mva1269I GAATGC 2 cut(s) 65, 1232
NlaIII CATG 5 cut(s) 59, 350, 470, 526, 1186
NlaIV GGNNCC 4 cut(s) 127, 487, 562, 814
NspV TTCGAA 1 cut(s) 1236
OliI CACNNNNGTG 2 cut(s) 801, 1088
PaeR7I CTCGAG 1 cut(s) 1046
PciSI GCTCTTC 1 cut(s) 336
PctI GAATGC 2 cut(s) 65, 1232
PdmI GAANNNNTTC 1 cut(s) 1443
PfeI GAWTC 5 cut(s) 46, 914, 1019, 1148, 1271
PflFI GACNNNGTC 2 cut(s) 1105, 1469
PkrI GCNGC 3 cut(s) 590, 1003, 1098
PleI GAGTC 3 cut(s) 175, 1227, 1300
PpsI GAGTC 3 cut(s) 175, 1227, 1300
PpuMI RGGWCCY 1 cut(s) 812
Psp124BI GAGCTC 1 cut(s) 1389
Psp1406I AACGTT 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 812
PspFI CCCAGC 1 cut(s) 453
PspN4I GGNNCC 4 cut(s) 127, 487, 562, 814
PspPI GGNCC 2 cut(s) 683, 812
PspPPI RGGWCCY 1 cut(s) 812
PspXI VCTCGAGB 1 cut(s) 1046
PsuI RGATCY 6 cut(s) 167, 190, 485, 602, 703, 1378
PsyI GACNNNGTC 2 cut(s) 1105, 1469
PvuII CAGCTG 1 cut(s) 1099
RsaI GTAC 3 cut(s) 53, 639, 938
RsaNI GTAC 3 cut(s) 52, 638, 937
RseI CAYNNNNRTG 3 cut(s) 351, 801, 1088
SacI GAGCTC 1 cut(s) 1389
SapI GCTCTTC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 3 cut(s) 589, 1002, 1097
Sau96I GGNCC 2 cut(s) 683, 812
SchI GAGTC 3 cut(s) 175, 1228, 1301
SduI GDGCHC 4 cut(s) 24, 1201, 1369, 1389
SfaNI GCATC 4 cut(s) 223, 883, 1234, 1327
Sfr274I CTCGAG 1 cut(s) 1046
SfuI TTCGAA 1 cut(s) 1236
SinI GGWCC 2 cut(s) 683, 812
SlaI CTCGAG 1 cut(s) 1046
SmiMI CAYNNNNRTG 3 cut(s) 351, 801, 1088
SmlI CTYRAG 6 cut(s) 186, 618, 693, 1046, 1136, 1382
SmoI CTYRAG 6 cut(s) 186, 618, 693, 1046, 1136, 1382
Sse9I AATT 9 cut(s) 70, 84, 540, 650, 759, 907, 1013, 1023, 1441
SsiI CCGC 2 cut(s) 589, 686
SspMI CTAG 2 cut(s) 1241, 1289
SstI GAGCTC 1 cut(s) 1389
StyI CCWWGG 1 cut(s) 1431
TaaI ACNGT 2 cut(s) 941, 1256
TaiI ACGT 1 cut(s) 42
TaqI TCGA 3 cut(s) 1047, 1228, 1236
TaqII GACCGA 1 cut(s) 225
TasI AATT 9 cut(s) 70, 84, 540, 650, 759, 907, 1013, 1023, 1441
TauI GCSGC 1 cut(s) 591
TfiI GAWTC 5 cut(s) 46, 914, 1019, 1148, 1271
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 4 cut(s) 70, 750, 823, 925
TseI GCWGC 2 cut(s) 1001, 1096
TspDTI ATGAA 6 cut(s) 72, 363, 363, 483, 552, 1206
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 4 cut(s) 70, 750, 823, 925
Tth111I GACNNNGTC 2 cut(s) 1105, 1469
VneI GTGCAC 2 cut(s) 20, 1197
VpaK11BI GGWCC 2 cut(s) 683, 812
XapI RAATTY 1 cut(s) 1441
XcmI CCANNNNNNNNNTGG 1 cut(s) 925
XhoI CTCGAG 1 cut(s) 1046
XmnI GAANNNNTTC 1 cut(s) 1443
XspI CTAG 2 cut(s) 1241, 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.