RLG00000013229

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002091
Physical Location & Seq
Forward (+)
28033542 .. 28037481
3940 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013229

Sequence Viewer

Length: 1728 bp
ATGGCACCAGCAATGGTGGCTCTGGCACACCTAGAATGGTTTGTCTTCGTTCAAAACAAGACTGCTCCTCCACCAACTTATTACTGCTCTGGCCGCACGATTTGCGCTTTTGGGTTCCATAATCGCCTACCAGAGAAGAAACCCACTTCGTCTCTCGCTCTGACCCTTCTGTTTTGGTTTGTTGGGAAAGGCCGGAAGAATAAAAAGAAATCAGATAAGGGCGGTGATATAAAAGATGGTGAGTTCAACATTAAGATGGCTGGAGAGAGAAGAGTGGATGCAACTAGGGCATCTAATCCTTACTATCAATCTGGTCAATCTTTTCCGAAAAGAACTGCCGGTGGTGAAGCTCGTGGGGATATAAAAGAATCAGGCTCCATACTTGATGTTCCTATATTGGGTGGACGAAAAGAGAGATCCCGACCAAAGACTCCTCAAGATCTTGACAATGTATCTGACCGAAGTGTGGTTTATCCTTATGATGCTCAATCATCTCCATCTAAGGACAAGGTGAAAGTAGAGAATGGGTCTGTCCTTGATGATCCTATATTTGGAAAAAGAAAGCCAGTCTCCCAAACAAGGTCTGCTGAAGAGCATGAAAATGGTCAAGGTGTTGATCCTTCATCATTTCCAGAAAAACTGAAAATAGAGAACGGCTCTGCCCTTGATGATCCTATATTTGGCAGAAGAAAGCAGGTAGCCCAGCCAAAGCCCCATGAAGAGCTTGACAATGGATCCGGTCAAGGTTCAGTTAATCCTTCTGGTTCTCCCATGTCTCCATCTAATGAAATTGTGAAAATAGGGAATGGCTCCACCCTTGATGATCTTATATTTGGCCGCAAAAAGCAGGGATCTGAACCAAAGCCTCAAGAAGAGATTGAGAATGTACCTGTTATGAAATTAGATAATGATGAGTATAAGTCTTATTCTGGACCGCTTGCTCAAGTAAAAGATCCTTCTTTGAACAAGAAGCAAATCAATTCCTCTGGGGCAGTGCCTACATCTGGAATTGTCAAAATGACCCCCTCTCTTTCTCTCCCCACTCACAGTGGTGGAGAAGGTCCCACTGCCGCACCTTCTGAAAACAGAAGCTTCAGTTTCTCAGGTTCTCCCTATGTTTTGGAAGAGAGTGATGATGACGATGCACGATCTGTCAATTCTGATTCCAGTGTTTCTGTGGGAAAGTACCGTGTGAAAGGAAGCTTATGTTCCATTCTCCAGTCAATCTTTGACAAGTATGGTGATATAGCAGCAAGCTGTCAATTGGAATCAATTGTCATTCGCTCTTATTACCTCGAGTGTGTATGTTATGTGGTTCAAGAGTTGAAACGCACTTCGGTGAAGCAGCTGACAAAGTCAAAAGTCAAAGAAATGTCAGCTATGCTCAAGGATGTAGAATCTTCAGGAATGGATGTTGGTTGGCTTCGTCTCATGCTCAATGAATGTGCACAATCCATTGATCTCGTGAGTCAGCATCGAGCATTTGAACTAGAAAAGGCCAACTGTGATCGTGGCATTGAATCAATAAGGAAAGAACTAGAGTCCCAGATGGAGGCTTTGGCTCTGAAAGAAAAGGAGGTTGCTGATGCCAAGAAGCAGGTTTCTGAAACCAGAGCCCATCTCAGGGAGCTGGAGCTGAAATCATCTATTTTGAGTGAGACTGTTAGTAATCTGAAGTCCAAGGTTGAAAATCTTCAAAGCAAATCATTGTTGGACAAGGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

576

Amino Acids

63.07

Weight (kDa)

8.19

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 374 - 572 1.7e-64 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 685, 1588
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 5 cut(s) 94, 222, 838, 935, 1071
AclWI GGATC 8 cut(s) 411, 536, 611, 665, 729, 742, 859, 947
AcoI YGGCCR 2 cut(s) 91, 835
AcuI CTGAAG 4 cut(s) 609, 1078, 1386, 1694
AfaI GTAC 2 cut(s) 888, 1187
AfiI CCNNNNNNNGG 9 cut(s) 398, 466, 551, 579, 680, 1004, 1552, 1623, 1624
AgsI TTSAA 9 cut(s) 53, 247, 964, 1319, 1327, 1487, 1520, 1688, 1697
AleI CACNNNNGTG 2 cut(s) 1050, 1337
AluBI AGCT 9 cut(s) 350, 724, 1092, 1203, 1257, 1348, 1379, 1630, 1636
AluI AGCT 9 cut(s) 350, 724, 1092, 1203, 1257, 1348, 1379, 1630, 1636
Alw21I GWGCWC 1 cut(s) 1450
Alw26I GTCTC 5 cut(s) 156, 574, 780, 1433, 1652
Alw44I GTGCAC 1 cut(s) 1446
AlwI GGATC 8 cut(s) 411, 536, 611, 665, 729, 742, 859, 947
Ama87I CYCGRG 1 cut(s) 1295
AoxI GGCC 4 cut(s) 91, 190, 835, 1497
ApaLI GTGCAC 1 cut(s) 1446
ApeKI GCWGC 2 cut(s) 1250, 1345
Asp700I GAANNNNTTC 1 cut(s) 1692
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 2 cut(s) 932, 1061
AsuHPI GGTGA 6 cut(s) 236, 251, 356, 523, 1253, 1351
AvaI CYCGRG 1 cut(s) 1295
AvaII GGWCC 2 cut(s) 932, 1061
BaeGI GKGCMC 1 cut(s) 1450
BamHI GGATCC 1 cut(s) 734
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 1618
BauI CACGAG 2 cut(s) 351, 1463
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 1450
BbvI GCAGC 2 cut(s) 1262, 1357
BccI CCATC 6 cut(s) 230, 250, 505, 787, 1543, 1626
BceAI ACGGC 1 cut(s) 670
BcoDI GTCTC 5 cut(s) 156, 574, 780, 1433, 1652
BfaI CTAG 4 cut(s) 32, 285, 1490, 1538
BfuAI ACCTGC 2 cut(s) 685, 1588
BglII AGATCT 1 cut(s) 439
BisI GCNGC 5 cut(s) 94, 838, 1071, 1251, 1346
BlsI GCNGC 5 cut(s) 95, 839, 1072, 1252, 1347
Bme18I GGWCC 2 cut(s) 932, 1061
BmeT110I CYCGRG 1 cut(s) 1295
BmgT120I GGNCC 2 cut(s) 932, 1061
BmiI GGNNCC 6 cut(s) 6, 116, 376, 736, 811, 1063
BmsI GCATC 6 cut(s) 268, 299, 472, 1132, 1483, 1576
BpiI GAAGAC 1 cut(s) 37
BplI GAGNNNNNCTC 4 cut(s) 641, 673, 1605, 1637
BpmI CTGGAG 3 cut(s) 282, 1202, 1652
BpuEI CTTGAG 4 cut(s) 420, 852, 927, 1370
BsaJI CCNNGG 1 cut(s) 1680
BsaWI WCCGGW 1 cut(s) 737
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 9 cut(s) 398, 466, 551, 579, 680, 1004, 1552, 1623, 1624
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 3 cut(s) 566, 1167, 1219
Bse3DI GCAATG 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 1680
BseGI GGATG 3 cut(s) 283, 1396, 1417
BseLI CCNNNNNNNGG 9 cut(s) 398, 466, 551, 579, 680, 1004, 1552, 1623, 1624
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 2 cut(s) 1116, 1636
BseNI ACTGG 3 cut(s) 566, 1167, 1219
BseRI GAGGAG 2 cut(s) 57, 423
BseSI GKGCMC 1 cut(s) 1450
BseXI GCAGC 2 cut(s) 1262, 1357
BseYI CCCAGC 1 cut(s) 702
BshFI GGCC 4 cut(s) 93, 192, 837, 1499
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 1450
BsiHKCI CYCGRG 1 cut(s) 1295
BsiSI CCGG 3 cut(s) 193, 339, 738
BslFI GGGAC 2 cut(s) 1047, 1528
BslI CCNNNNNNNGG 9 cut(s) 398, 466, 551, 579, 680, 1004, 1552, 1623, 1624
BsmAI GTCTC 5 cut(s) 156, 574, 780, 1433, 1652
BsmBI CGTCTC 2 cut(s) 156, 1433
BsmFI GGGAC 2 cut(s) 1047, 1528
BsnI GGCC 4 cut(s) 93, 192, 837, 1499
BsoBI CYCGRG 1 cut(s) 1295
Bsp1286I GDGCHC 2 cut(s) 1450, 1618
BspACI CCGC 5 cut(s) 94, 222, 838, 935, 1071
BspANI GGCC 4 cut(s) 93, 192, 837, 1499
BspCNI CTCAG 2 cut(s) 1115, 1635
BspLI GGNNCC 6 cut(s) 6, 116, 376, 736, 811, 1063
BspMI ACCTGC 2 cut(s) 685, 1588
BspPI GGATC 8 cut(s) 411, 536, 611, 665, 729, 742, 859, 947
BspQI GCTCTTC 2 cut(s) 585, 714
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 3 cut(s) 566, 1167, 1219
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 1 cut(s) 1680
BssSI CACGAG 2 cut(s) 351, 1463
BssT1I CCWWGG 1 cut(s) 1680
Bst2BI CACGAG 2 cut(s) 351, 1463
Bst4CI ACNGT 4 cut(s) 1049, 1190, 1505, 1663
Bst6I CTCTTC 5 cut(s) 265, 585, 714, 867, 1119
BstAPI GCANNNNNTGC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 939, 1255
BstDEI CTNAG 3 cut(s) 501, 1102, 1622
BstF5I GGATG 3 cut(s) 283, 1396, 1417
BstHHI GCGC 1 cut(s) 107
BstMAI GTCTC 5 cut(s) 156, 574, 780, 1433, 1652
BstMWI GCNNNNNNNGC 4 cut(s) 17, 93, 102, 287
BstSLI GKGCMC 1 cut(s) 1450
BstV1I GCAGC 2 cut(s) 1262, 1357
BstV2I GAAGAC 1 cut(s) 37
BstX2I RGATCY 5 cut(s) 416, 439, 734, 851, 952
BstYI RGATCY 5 cut(s) 416, 439, 734, 851, 952
BsuRI GGCC 4 cut(s) 93, 192, 837, 1499
BtsCI GGATG 3 cut(s) 283, 1396, 1417
BtsI GCAGTG 2 cut(s) 999, 1065
BtsIMutI CAGTG 4 cut(s) 999, 1054, 1065, 1174
BveI ACCTGC 2 cut(s) 685, 1588
Cac8I GCNNGC 2 cut(s) 939, 1255
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 932, 1061
Csp6I GTAC 2 cut(s) 887, 1186
CviAII CATG 4 cut(s) 596, 716, 772, 1432
CviQI GTAC 2 cut(s) 887, 1186
DdeI CTNAG 3 cut(s) 501, 1102, 1622
EaeI YGGCCR 2 cut(s) 91, 835
Eam1104I CTCTTC 5 cut(s) 265, 585, 714, 867, 1119
EarI CTCTTC 5 cut(s) 265, 585, 714, 867, 1119
Eco130I CCWWGG 1 cut(s) 1680
Eco24I GRGCYC 1 cut(s) 1618
Eco47I GGWCC 2 cut(s) 932, 1061
Eco57I CTGAAG 4 cut(s) 609, 1078, 1386, 1694
Eco88I CYCGRG 1 cut(s) 1295
EcoO109I RGGNCCY 1 cut(s) 1061
EcoT14I CCWWGG 1 cut(s) 1680
EcoT38I GRGCYC 1 cut(s) 1618
ErhI CCWWGG 1 cut(s) 1680
Esp3I CGTCTC 2 cut(s) 156, 1433
FaeI CATG 4 cut(s) 599, 719, 775, 1435
FaqI GGGAC 2 cut(s) 1047, 1528
FatI CATG 4 cut(s) 595, 715, 771, 1431
Fnu4HI GCNGC 5 cut(s) 94, 838, 1071, 1251, 1346
FokI GGATG 3 cut(s) 290, 1403, 1424
FriOI GRGCYC 1 cut(s) 1618
Fsp4HI GCNGC 5 cut(s) 94, 838, 1071, 1251, 1346
FspBI CTAG 4 cut(s) 32, 285, 1490, 1538
GlaI GCGC 1 cut(s) 106
GluI GCNGC 5 cut(s) 94, 838, 1071, 1251, 1346
GsaI CCCAGC 1 cut(s) 706
GsuI CTGGAG 3 cut(s) 282, 1202, 1652
HaeIII GGCC 4 cut(s) 93, 192, 837, 1499
HapII CCGG 3 cut(s) 193, 339, 738
HhaI GCGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 599, 719, 775, 1435
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HindIII AAGCTT 2 cut(s) 1090, 1201
HinfI GANTC 8 cut(s) 368, 430, 1163, 1268, 1397, 1468, 1520, 1541
HpaII CCGG 3 cut(s) 193, 339, 738
HphI GGTGA 6 cut(s) 236, 251, 356, 523, 1253, 1351
Hpy166II GTNNAC 2 cut(s) 404, 1448
Hpy8I GTNNAC 2 cut(s) 404, 1448
HpyAV CCTTC 6 cut(s) 176, 630, 768, 966, 1052, 1086
HpyCH4III ACNGT 4 cut(s) 1049, 1190, 1505, 1663
HpyCH4V TGCA 3 cut(s) 281, 1145, 1448
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 93, 102, 287
HpyF3I CTNAG 3 cut(s) 501, 1102, 1622
Hsp92II CATG 4 cut(s) 599, 719, 775, 1435
HspAI GCGC 1 cut(s) 105
LguI GCTCTTC 2 cut(s) 585, 714
LmnI GCTCC 5 cut(s) 70, 380, 815, 1627, 1633
Lsp1109I GCAGC 2 cut(s) 1262, 1357
LweI GCATC 6 cut(s) 268, 299, 472, 1132, 1483, 1576
MaeI CTAG 4 cut(s) 32, 285, 1490, 1538
MfeI CAATTG 2 cut(s) 1262, 1272
MflI RGATCY 5 cut(s) 416, 439, 734, 851, 952
MhlI GDGCHC 2 cut(s) 1450, 1618
MluCI AATT 7 cut(s) 789, 899, 979, 1008, 1156, 1262, 1272
MlyI GAGTC 3 cut(s) 424, 1477, 1550
MmeI TCCRAC 1 cut(s) 1692
MnlI CCTC 8 cut(s) 78, 444, 876, 994, 1036, 1304, 1546, 1570
MroXI GAANNNNTTC 1 cut(s) 1692
MseI TTAA 2 cut(s) 252, 753
MslI CAYNNNNRTG 4 cut(s) 254, 600, 1050, 1337
MspA1I CMGCKG 1 cut(s) 1348
MspI CCGG 3 cut(s) 193, 339, 738
MunI CAATTG 2 cut(s) 1262, 1272
MwoI GCNNNNNNNGC 4 cut(s) 17, 93, 102, 287
NlaIII CATG 4 cut(s) 599, 719, 775, 1435
NlaIV GGNNCC 6 cut(s) 6, 116, 376, 736, 811, 1063
OliI CACNNNNGTG 2 cut(s) 1050, 1337
PaeR7I CTCGAG 1 cut(s) 1295
PciSI GCTCTTC 2 cut(s) 585, 714
PdmI GAANNNNTTC 1 cut(s) 1692
PfeI GAWTC 5 cut(s) 368, 1163, 1268, 1397, 1520
PflFI GACNNNGTC 2 cut(s) 1354, 1718
PkrI GCNGC 5 cut(s) 95, 839, 1072, 1252, 1347
PleI GAGTC 3 cut(s) 424, 1476, 1549
PpsI GAGTC 3 cut(s) 424, 1476, 1549
PpuMI RGGWCCY 1 cut(s) 1061
Psp5II RGGWCCY 1 cut(s) 1061
PspFI CCCAGC 1 cut(s) 702
PspN4I GGNNCC 6 cut(s) 6, 116, 376, 736, 811, 1063
PspPI GGNCC 2 cut(s) 932, 1061
PspPPI RGGWCCY 1 cut(s) 1061
PspXI VCTCGAGB 1 cut(s) 1295
PsuI RGATCY 5 cut(s) 416, 439, 734, 851, 952
PsyI GACNNNGTC 2 cut(s) 1354, 1718
PvuII CAGCTG 1 cut(s) 1348
RsaI GTAC 2 cut(s) 888, 1187
RsaNI GTAC 2 cut(s) 887, 1186
RseI CAYNNNNRTG 4 cut(s) 254, 600, 1050, 1337
SapI GCTCTTC 2 cut(s) 585, 714
SaqAI TTAA 2 cut(s) 252, 753
SatI GCNGC 5 cut(s) 94, 838, 1071, 1251, 1346
Sau96I GGNCC 2 cut(s) 932, 1061
SchI GAGTC 3 cut(s) 424, 1477, 1550
SduI GDGCHC 2 cut(s) 1450, 1618
SfaNI GCATC 6 cut(s) 268, 299, 472, 1132, 1483, 1576
Sfr274I CTCGAG 1 cut(s) 1295
SinI GGWCC 2 cut(s) 932, 1061
SlaI CTCGAG 1 cut(s) 1295
SmiMI CAYNNNNRTG 4 cut(s) 254, 600, 1050, 1337
SmlI CTYRAG 5 cut(s) 435, 867, 942, 1295, 1385
SmoI CTYRAG 5 cut(s) 435, 867, 942, 1295, 1385
Sse9I AATT 7 cut(s) 789, 899, 979, 1008, 1156, 1262, 1272
SsiI CCGC 5 cut(s) 94, 222, 838, 935, 1071
SspMI CTAG 4 cut(s) 32, 285, 1490, 1538
StyI CCWWGG 1 cut(s) 1680
TaaI ACNGT 4 cut(s) 1049, 1190, 1505, 1663
TaqI TCGA 2 cut(s) 1296, 1477
TaqII GACCGA 1 cut(s) 474
TasI AATT 7 cut(s) 789, 899, 979, 1008, 1156, 1262, 1272
TauI GCSGC 3 cut(s) 96, 840, 1073
TfiI GAWTC 5 cut(s) 368, 1163, 1268, 1397, 1520
Tru1I TTAA 2 cut(s) 252, 753
Tru9I TTAA 2 cut(s) 252, 753
TscAI CASTG 4 cut(s) 999, 1054, 1072, 1174
TseI GCWGC 2 cut(s) 1250, 1345
TspDTI ATGAA 6 cut(s) 612, 612, 732, 801, 911, 1455
TspRI CASTG 4 cut(s) 999, 1054, 1072, 1174
Tth111I GACNNNGTC 2 cut(s) 1354, 1718
VneI GTGCAC 1 cut(s) 1446
VpaK11BI GGWCC 2 cut(s) 932, 1061
XcmI CCANNNNNNNNNTGG 1 cut(s) 1174
XhoI CTCGAG 1 cut(s) 1295
XmnI GAANNNNTTC 1 cut(s) 1692
XspI CTAG 4 cut(s) 32, 285, 1490, 1538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.