Rw6G019730

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002091
Physical Location & Seq
Reverse (-)
39437569 .. 39442086
4518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G019730.1

Sequence Viewer

Length: 1404 bp
ATGGATTTTAATAATTCCTACCTTTTTTCAATGGCTATTTTAATTGCAGGCTCCATACTTGATGTTCCTATATTGGGTGGACGAAAAGAGAGATCCCGACCAAAGACTCCTCAAGATCTTGACAATGTATCTGACCGAAGTGCGGTTTATCCTTATGATGCTCAATCATCTCCATCTAAGGACAAGGTGAACGTAGAGAATGGGTCTGTCCTTGATGATCCTATATTTGGAAAAAGAAAGCCAGTCTCCCAAACAAGGTCTGCTGAAGAGCATGAAAATGGTCAAGGTGTTGATCCTTCATCATTTCCAGAAAAGCTGAAAATAGAGAACGGCTCTGCCCTTGATGATCCTATATTTGGCAGAAGAAAGCAGGTAGCCCAGCCAAAGCCCCATGAAGAGCTTGACAATGGATCCGGTCAAGGTTCAGTTAATCCTTCTGGTTCTCCCATGTCTCCATCTAATGAAATTGTGAAAATAGGGAATGGCTCCACTCTTGATGATCTTATATTTGGCCGCAAAAAGCAGGGATCTGAACCAAAGCCTGAAGAAGAGATTGAGAATGTACCTGTTGTGAAATTAGATAATGATGAGTATAAGTCTTATTCTGGACCGCTTGCTCAAGTAAAAGATCCTTCTTTGAACAAGAAGCAAATCAATTCCTCTGGGGCAGTGCCTGCATCTGGAATTATCAAAATGACCCCCTCTCTTTCTCTCCCCACACACAATGGTGGAGAAGGTCCCACTGCCACACCTTCTGAAAACAGAAGCTTCAGTTTCTCAGGTTCTCCCTATGTTTTTGAAGAGAGTGATGATGACGATGCACGATCTGTCAATTCTGATGCCAGTGTTTCTGTGGGAAAGTACCGTGTGAAAGGAAGCTTATGTTCCATTCTCCAGTCAATCTTTGACAAGTATGGTGATATAGCAGCAAGCTGTCAATTGGAATCAATTGTCATTCGCTCTTATTACCTCGAGTGTGTCTGTTATGTGGTTCAAGAGTTGAAACGCACTTCGGTGAAGCAGCTGACAAAGTCAAAAGTCAAAGAAATGTCAGCTATGCTCAAGGATGTAGAATCTTCAGGAATGGATGTTGGTTGGCTTCGTCTCATGCTCAATGAATGTGCACAATCCATTGATCTCGTGAGTCAGCATCGAGCATTTGAACTAGAAAAGGCCAACTGTGATCGTGGCATTGAATCAATAAGGAAAGAACTAGAGTCCCAGATGGAGGCTTTGGCTCTGAAAGAAAAGGAGGTTGCTGATGCCAAGAAGCAAGTTTCTGAAACCAGAGCCCATCTGAGGGAGCTTGAGCTCAAATCATCTTTTTTGAGTGAGACTGTTAGTAATCTGAAGTCAAAGGTTGAAAATCTTCAAAGCAAATCATTGCTGGACAAGGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

467

Amino Acids

51.16

Weight (kDa)

5.34

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 272 - 464 7.1e-65 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 361
AciI CCGC 3 cut(s) 143, 514, 611
AclWI GGATC 8 cut(s) 87, 212, 287, 341, 405, 418, 535, 623
AcoI YGGCCR 1 cut(s) 511
AcuI CTGAAG 5 cut(s) 285, 564, 754, 1062, 1370
AfaI GTAC 2 cut(s) 564, 863
AfiI CCNNNNNNNGG 9 cut(s) 74, 142, 227, 255, 356, 680, 1228, 1299, 1300
AgsI TTSAA 9 cut(s) 30, 640, 800, 995, 1003, 1163, 1196, 1364, 1373
AleI CACNNNNGTG 2 cut(s) 726, 1013
AluBI AGCT 9 cut(s) 316, 400, 768, 879, 933, 1024, 1055, 1306, 1312
AluI AGCT 9 cut(s) 316, 400, 768, 879, 933, 1024, 1055, 1306, 1312
Alw21I GWGCWC 2 cut(s) 1126, 1314
Alw26I GTCTC 4 cut(s) 250, 456, 1109, 1328
Alw44I GTGCAC 1 cut(s) 1122
AlwI GGATC 8 cut(s) 87, 212, 287, 341, 405, 418, 535, 623
AlwNI CAGNNNCTG 1 cut(s) 674
Ama87I CYCGRG 1 cut(s) 971
AoxI GGCC 2 cut(s) 511, 1173
ApaLI GTGCAC 1 cut(s) 1122
ApeKI GCWGC 2 cut(s) 926, 1021
Asp700I GAANNNNTTC 1 cut(s) 1368
AspS9I GGNCC 2 cut(s) 608, 737
AsuHPI GGTGA 3 cut(s) 199, 929, 1027
AvaI CYCGRG 1 cut(s) 971
AvaII GGWCC 2 cut(s) 608, 737
BaeGI GKGCMC 1 cut(s) 1126
BamHI GGATCC 1 cut(s) 410
BanII GRGCYC 2 cut(s) 1294, 1314
BauI CACGAG 1 cut(s) 1139
Bbv12I GWGCWC 2 cut(s) 1126, 1314
BbvI GCAGC 2 cut(s) 938, 1033
BccI CCATC 4 cut(s) 181, 463, 1219, 1302
BceAI ACGGC 1 cut(s) 346
BcoDI GTCTC 4 cut(s) 250, 456, 1109, 1328
BfaI CTAG 2 cut(s) 1166, 1214
BfuAI ACCTGC 1 cut(s) 361
BglII AGATCT 1 cut(s) 115
BisI GCNGC 3 cut(s) 514, 927, 1022
BlsI GCNGC 3 cut(s) 515, 928, 1023
Bme18I GGWCC 2 cut(s) 608, 737
BmeT110I CYCGRG 1 cut(s) 971
BmgT120I GGNCC 2 cut(s) 608, 737
BmiI GGNNCC 4 cut(s) 52, 412, 487, 739
BmsI GCATC 6 cut(s) 148, 686, 808, 829, 1159, 1252
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 878
BpuEI CTTGAG 4 cut(s) 96, 603, 1046, 1328
BsaWI WCCGGW 1 cut(s) 413
Bsc4I CCNNNNNNNGG 9 cut(s) 74, 142, 227, 255, 356, 680, 1228, 1299, 1300
Bse1I ACTGG 3 cut(s) 242, 843, 895
Bse3DI GCAATG 1 cut(s) 1382
BseGI GGATG 2 cut(s) 1072, 1093
BseLI CCNNNNNNNGG 9 cut(s) 74, 142, 227, 255, 356, 680, 1228, 1299, 1300
BseMI GCAATG 1 cut(s) 1382
BseMII CTCAG 2 cut(s) 792, 1289
BseNI ACTGG 3 cut(s) 242, 843, 895
BseRI GAGGAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 1126
BseXI GCAGC 2 cut(s) 938, 1033
BseYI CCCAGC 1 cut(s) 378
BshFI GGCC 2 cut(s) 513, 1175
BsiHKAI GWGCWC 2 cut(s) 1126, 1314
BsiHKCI CYCGRG 1 cut(s) 971
BsiSI CCGG 1 cut(s) 414
BslFI GGGAC 2 cut(s) 723, 1204
BslI CCNNNNNNNGG 9 cut(s) 74, 142, 227, 255, 356, 680, 1228, 1299, 1300
BsmAI GTCTC 4 cut(s) 250, 456, 1109, 1328
BsmBI CGTCTC 1 cut(s) 1109
BsmFI GGGAC 2 cut(s) 723, 1204
BsnI GGCC 2 cut(s) 513, 1175
BsoBI CYCGRG 1 cut(s) 971
Bsp1286I GDGCHC 3 cut(s) 1126, 1294, 1314
BspACI CCGC 3 cut(s) 143, 514, 611
BspANI GGCC 2 cut(s) 513, 1175
BspCNI CTCAG 2 cut(s) 791, 1290
BspLI GGNNCC 4 cut(s) 52, 412, 487, 739
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 8 cut(s) 87, 212, 287, 341, 405, 418, 535, 623
BspQI GCTCTTC 2 cut(s) 261, 390
BsrDI GCAATG 1 cut(s) 1382
BsrI ACTGG 3 cut(s) 242, 843, 895
BssSI CACGAG 1 cut(s) 1139
Bst2BI CACGAG 1 cut(s) 1139
Bst4CI ACNGT 3 cut(s) 866, 1181, 1339
Bst6I CTCTTC 4 cut(s) 261, 390, 543, 795
BstAPI GCANNNNNTGC 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 49, 615, 675, 931
BstDEI CTNAG 3 cut(s) 177, 778, 1298
BstF5I GGATG 2 cut(s) 1072, 1093
BstMAI GTCTC 4 cut(s) 250, 456, 1109, 1328
BstMWI GCNNNNNNNGC 1 cut(s) 674
BstSLI GKGCMC 1 cut(s) 1126
BstV1I GCAGC 2 cut(s) 938, 1033
BstX2I RGATCY 5 cut(s) 92, 115, 410, 527, 628
BstYI RGATCY 5 cut(s) 92, 115, 410, 527, 628
BsuRI GGCC 2 cut(s) 513, 1175
BtsCI GGATG 2 cut(s) 1072, 1093
BtsI GCAGTG 2 cut(s) 675, 741
BtsIMutI CAGTG 3 cut(s) 675, 741, 850
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 4 cut(s) 49, 615, 675, 931
CaiI CAGNNNCTG 1 cut(s) 674
Cfr13I GGNCC 2 cut(s) 608, 737
Csp6I GTAC 2 cut(s) 563, 862
CviAII CATG 4 cut(s) 272, 392, 448, 1108
CviQI GTAC 2 cut(s) 563, 862
DdeI CTNAG 3 cut(s) 177, 778, 1298
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 4 cut(s) 261, 390, 543, 795
EarI CTCTTC 4 cut(s) 261, 390, 543, 795
Ecl136II GAGCTC 1 cut(s) 1312
Eco24I GRGCYC 2 cut(s) 1294, 1314
Eco47I GGWCC 2 cut(s) 608, 737
Eco53kI GAGCTC 1 cut(s) 1312
Eco57I CTGAAG 5 cut(s) 285, 564, 754, 1062, 1370
Eco88I CYCGRG 1 cut(s) 971
EcoICRI GAGCTC 1 cut(s) 1312
EcoO109I RGGNCCY 1 cut(s) 737
EcoT38I GRGCYC 2 cut(s) 1294, 1314
Esp3I CGTCTC 1 cut(s) 1109
FaeI CATG 4 cut(s) 275, 395, 451, 1111
FaqI GGGAC 2 cut(s) 723, 1204
FatI CATG 4 cut(s) 271, 391, 447, 1107
Fnu4HI GCNGC 3 cut(s) 514, 927, 1022
FokI GGATG 2 cut(s) 1079, 1100
FriOI GRGCYC 2 cut(s) 1294, 1314
Fsp4HI GCNGC 3 cut(s) 514, 927, 1022
FspBI CTAG 2 cut(s) 1166, 1214
GluI GCNGC 3 cut(s) 514, 927, 1022
GsaI CCCAGC 1 cut(s) 382
GsuI CTGGAG 1 cut(s) 878
HaeIII GGCC 2 cut(s) 513, 1175
HapII CCGG 1 cut(s) 414
Hin1II CATG 4 cut(s) 275, 395, 451, 1111
HindIII AAGCTT 2 cut(s) 766, 877
HinfI GANTC 6 cut(s) 106, 944, 1073, 1144, 1196, 1217
HpaII CCGG 1 cut(s) 414
HphI GGTGA 3 cut(s) 199, 929, 1027
Hpy166II GTNNAC 3 cut(s) 80, 190, 1124
Hpy188I TCNGA 8 cut(s) 133, 532, 757, 838, 1242, 1282, 1299, 1350
Hpy8I GTNNAC 3 cut(s) 80, 190, 1124
HpyAV CCTTC 5 cut(s) 306, 444, 642, 728, 762
HpyCH4III ACNGT 3 cut(s) 866, 1181, 1339
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 4 cut(s) 47, 677, 821, 1124
HpyF10VI GCNNNNNNNGC 1 cut(s) 674
HpyF3I CTNAG 3 cut(s) 177, 778, 1298
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 4 cut(s) 275, 395, 451, 1111
LguI GCTCTTC 2 cut(s) 261, 390
LmnI GCTCC 3 cut(s) 56, 491, 1303
Lsp1109I GCAGC 2 cut(s) 938, 1033
LweI GCATC 6 cut(s) 148, 686, 808, 829, 1159, 1252
MaeI CTAG 2 cut(s) 1166, 1214
MaeII ACGT 1 cut(s) 192
MboII GAAGA 8 cut(s) 278, 375, 407, 557, 560, 812, 1068, 1361
MfeI CAATTG 2 cut(s) 938, 948
MflI RGATCY 5 cut(s) 92, 115, 410, 527, 628
MhlI GDGCHC 3 cut(s) 1126, 1294, 1314
MluCI AATT 9 cut(s) 13, 42, 465, 575, 655, 684, 832, 938, 948
MlyI GAGTC 3 cut(s) 100, 1153, 1226
MnlI CCTC 7 cut(s) 120, 670, 712, 980, 1222, 1246, 1293
MroXI GAANNNNTTC 1 cut(s) 1368
MseI TTAA 3 cut(s) 9, 41, 429
MslI CAYNNNNRTG 3 cut(s) 276, 726, 1013
MspA1I CMGCKG 1 cut(s) 1024
MspI CCGG 1 cut(s) 414
MunI CAATTG 2 cut(s) 938, 948
MwoI GCNNNNNNNGC 1 cut(s) 674
NlaIII CATG 4 cut(s) 275, 395, 451, 1111
NlaIV GGNNCC 4 cut(s) 52, 412, 487, 739
OliI CACNNNNGTG 2 cut(s) 726, 1013
PaeR7I CTCGAG 1 cut(s) 971
PciSI GCTCTTC 2 cut(s) 261, 390
PdmI GAANNNNTTC 1 cut(s) 1368
PfeI GAWTC 3 cut(s) 944, 1073, 1196
PflFI GACNNNGTC 2 cut(s) 1030, 1394
PkrI GCNGC 3 cut(s) 515, 928, 1023
PleI GAGTC 3 cut(s) 100, 1152, 1225
PpsI GAGTC 3 cut(s) 100, 1152, 1225
PpuMI RGGWCCY 1 cut(s) 737
Psp124BI GAGCTC 1 cut(s) 1314
Psp5II RGGWCCY 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 378
PspN4I GGNNCC 4 cut(s) 52, 412, 487, 739
PspPI GGNCC 2 cut(s) 608, 737
PspPPI RGGWCCY 1 cut(s) 737
PspXI VCTCGAGB 1 cut(s) 971
PstNI CAGNNNCTG 1 cut(s) 674
PsuI RGATCY 5 cut(s) 92, 115, 410, 527, 628
PsyI GACNNNGTC 2 cut(s) 1030, 1394
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 2 cut(s) 564, 863
RsaNI GTAC 2 cut(s) 563, 862
RseI CAYNNNNRTG 3 cut(s) 276, 726, 1013
SacI GAGCTC 1 cut(s) 1314
SapI GCTCTTC 2 cut(s) 261, 390
SaqAI TTAA 3 cut(s) 9, 41, 429
SatI GCNGC 3 cut(s) 514, 927, 1022
Sau96I GGNCC 2 cut(s) 608, 737
SchI GAGTC 3 cut(s) 100, 1153, 1226
SduI GDGCHC 3 cut(s) 1126, 1294, 1314
SfaNI GCATC 6 cut(s) 148, 686, 808, 829, 1159, 1252
Sfr274I CTCGAG 1 cut(s) 971
SinI GGWCC 2 cut(s) 608, 737
SlaI CTCGAG 1 cut(s) 971
SmiMI CAYNNNNRTG 3 cut(s) 276, 726, 1013
SmlI CTYRAG 5 cut(s) 111, 618, 971, 1061, 1307
SmoI CTYRAG 5 cut(s) 111, 618, 971, 1061, 1307
Sse9I AATT 9 cut(s) 13, 42, 465, 575, 655, 684, 832, 938, 948
SsiI CCGC 3 cut(s) 143, 514, 611
SspMI CTAG 2 cut(s) 1166, 1214
SstI GAGCTC 1 cut(s) 1314
TaaI ACNGT 3 cut(s) 866, 1181, 1339
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 972, 1153
TaqII GACCGA 1 cut(s) 150
TasI AATT 9 cut(s) 13, 42, 465, 575, 655, 684, 832, 938, 948
TauI GCSGC 1 cut(s) 516
TfiI GAWTC 3 cut(s) 944, 1073, 1196
Tru1I TTAA 3 cut(s) 9, 41, 429
Tru9I TTAA 3 cut(s) 9, 41, 429
TscAI CASTG 3 cut(s) 675, 748, 850
TseI GCWGC 2 cut(s) 926, 1021
TspDTI ATGAA 5 cut(s) 288, 288, 408, 477, 1131
TspRI CASTG 3 cut(s) 675, 748, 850
Tth111I GACNNNGTC 2 cut(s) 1030, 1394
VneI GTGCAC 1 cut(s) 1122
VpaK11BI GGWCC 2 cut(s) 608, 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 971
XmnI GAANNNNTTC 1 cut(s) 1368
XspI CTAG 2 cut(s) 1166, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.