RchiOBHm_Chr6g0278421

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002091
Physical Location & Seq
Reverse (-)
41546192 .. 41546971
780 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24975

Sequence Viewer

Length: 357 bp
ATGTCAGCTATGCTCAAGGATGTAGAATCTTCAGGAATGGATGTTGGTTGGCTTCGTCTTATGCTCAATGAATGTGCGCAATCCATTGATCTCGTGAGTCAGCATCGAGCATTCGAACTAGAAAAGGCCAACTGTGATCGTGGCATTGAATCAATAAGGAAAGAACTAGAGTCCCAGATGGAGGCTTTGGCTCTGAAAGAAAAGGAGGTTGCTGATGCCAAGAAGCAAGTTTCTGAAACCAGAGCCCATCTGAGGGAGCTTGAGCTCAAATCATCTGTTTTGAGTGAGACTGTTAGTAATCTGAAGTCCAAGGTTGAAAATCTTCAAAGCAAATCATTGTTGGACAAGGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

118

Amino Acids

13.28

Weight (kDa)

5.48

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 1 - 115 3.6e-25 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 78
AcuI CTGAAG 2 cut(s) 15, 323
AfiI CCNNNNNNNGG 3 cut(s) 181, 252, 253
AgsI TTSAA 3 cut(s) 149, 317, 326
AluBI AGCT 3 cut(s) 8, 259, 265
AluI AGCT 3 cut(s) 8, 259, 265
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 281
AoxI GGCC 1 cut(s) 126
Asp700I GAANNNNTTC 1 cut(s) 321
AspLEI GCGC 1 cut(s) 79
AsuII TTCGAA 1 cut(s) 114
BanII GRGCYC 2 cut(s) 247, 267
BauI CACGAG 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 267
BccI CCATC 2 cut(s) 172, 255
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 2 cut(s) 119, 167
BmsI GCATC 2 cut(s) 112, 205
Bpu14I TTCGAA 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 252, 253
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 2 cut(s) 25, 46
BseLI CCNNNNNNNGG 3 cut(s) 181, 252, 253
BseMII CTCAG 1 cut(s) 242
BshFI GGCC 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 267
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 3 cut(s) 181, 252, 253
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 157
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 1 cut(s) 128
Bsp119I TTCGAA 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 247, 267
Bsp143I GATC 2 cut(s) 88, 136
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 243
BspT104I TTCGAA 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 2 cut(s) 88, 136
BssSI CACGAG 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 92
Bst4CI ACNGT 2 cut(s) 134, 292
BstBI TTCGAA 1 cut(s) 114
BstDEI CTNAG 1 cut(s) 251
BstF5I GGATG 2 cut(s) 25, 46
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 2 cut(s) 91, 139
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 2 cut(s) 88, 136
BsuRI GGCC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 25, 46
CfoI GCGC 1 cut(s) 79
CviJI RGCY 8 cut(s) 8, 52, 128, 185, 191, 245, 259, 265
CviKI_1 RGCY 8 cut(s) 8, 52, 128, 185, 191, 245, 259, 265
DdeI CTNAG 1 cut(s) 251
DpnI GATC 2 cut(s) 90, 138
DpnII GATC 2 cut(s) 88, 136
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 2 cut(s) 247, 267
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 15, 323
EcoICRI GAGCTC 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 2 cut(s) 247, 267
ErhI CCWWGG 1 cut(s) 309
FaiI YATR 2 cut(s) 11, 62
FaqI GGGAC 1 cut(s) 157
FokI GGATG 2 cut(s) 32, 53
FriOI GRGCYC 2 cut(s) 247, 267
FspBI CTAG 2 cut(s) 119, 167
FspI TGCGCA 1 cut(s) 78
GlaI GCGC 1 cut(s) 78
HaeIII GGCC 1 cut(s) 128
HhaI GCGC 1 cut(s) 79
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 4 cut(s) 26, 97, 149, 170
Hpy188I TCNGA 4 cut(s) 195, 235, 252, 303
Hpy188III TCNNGA 2 cut(s) 33, 94
HpyCH4III ACNGT 2 cut(s) 134, 292
HpyF3I CTNAG 1 cut(s) 251
HspAI GCGC 1 cut(s) 77
Kzo9I GATC 2 cut(s) 88, 136
LmnI GCTCC 1 cut(s) 256
LpnPI CCDG 3 cut(s) 18, 188, 253
LweI GCATC 2 cut(s) 112, 205
MaeI CTAG 2 cut(s) 119, 167
MalI GATC 2 cut(s) 90, 138
MboI GATC 2 cut(s) 88, 136
MboII GAAGA 2 cut(s) 21, 314
MhlI GDGCHC 2 cut(s) 247, 267
MlyI GAGTC 2 cut(s) 106, 179
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 3 cut(s) 175, 199, 246
MroXI GAANNNNTTC 1 cut(s) 321
Mva1269I GAATGC 1 cut(s) 110
NdeII GATC 2 cut(s) 88, 136
NsbI TGCGCA 1 cut(s) 78
NspV TTCGAA 1 cut(s) 114
PctI GAATGC 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 26, 149
PflFI GACNNNGTC 1 cut(s) 347
PleI GAGTC 2 cut(s) 105, 178
PpsI GAGTC 2 cut(s) 105, 178
Psp124BI GAGCTC 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 347
SacI GAGCTC 1 cut(s) 267
Sau3AI GATC 2 cut(s) 88, 136
SchI GAGTC 2 cut(s) 106, 179
SduI GDGCHC 2 cut(s) 247, 267
SetI ASST 6 cut(s) 10, 210, 261, 267, 315, 351
SfaNI GCATC 2 cut(s) 112, 205
SfuI TTCGAA 1 cut(s) 114
SmlI CTYRAG 2 cut(s) 14, 260
SmoI CTYRAG 2 cut(s) 14, 260
SspMI CTAG 2 cut(s) 119, 167
SstI GAGCTC 1 cut(s) 267
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 2 cut(s) 134, 292
TaqI TCGA 2 cut(s) 106, 114
TfiI GAWTC 2 cut(s) 26, 149
TspDTI ATGAA 1 cut(s) 84
Tth111I GACNNNGTC 1 cut(s) 347
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 2 cut(s) 119, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.