MD10G1125400.v1.1

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002091
Physical Location & Seq
Reverse (-)
20521174 .. 20525491
4318 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1125400.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGTCTCTGATTGGAAGAGTACACCCCGAATGTGTAAACGTAGCCAATCCCTACCATGAATGCACTGAAAATTGCTTGAGAAAGATTGCTGAAGGGAAGGGCCGGAAGTTGACGAAAAAGAAATCAGATTTTGGCAGCAGCTTTAAAGATGTTGAGTACAAAAAAAAGGGGGCTGAGGACAAAAGAGCATCTAATCCTTACCATGACCCTGAATTCTACCGCCAAAGAGCCGCTGCTGCCGAACCTCGTGGGGACAAAAAAGAATCAGGTTCACTGCTTGATATTCCTATATTGGGCAGAAAAAAGGAGAGATCCCGACCAAACACCTCTCAGGAGCTTGACCGAAGTGTGATTTATCATTTAGATGCTCCATCTTCTCCGTCTAAGGAAAAGGTGAAATTAGAGAACGGCTCTGTCCTTGATGATCCTATATTTGGCAGAAGAAAGCAGGGATCTCAGCCAAAGCCCTCTGAAGAGCTTGACAATGTAACTGGTCGAGGTTTAATTTATCCCTCTGATTCTCCATCTAAAGAAATAGTGCAAGTAGAGAATGGCTCCGCACTTGATGATCCTATATTTGGCAGACGAAGGCCTGGTTCCAGACCAAAGAGCCCTGAAGAGCTTGACAATGTACCTGATGAAATTAAGGAAAAGGTTAAAATAGGGAATGGTGAGCATAAATCTTATTCTCCACAGGCTGCTGAAGTAAAAGATGCTACCTTCAACAAGGAGCAAGTTCCGTCCTCTCTGTTAGTGCCACCATCGGGAATTCTCACAATGCCTGAAAATCCCAAACACCCTCGAGAGAACGGTGCTACTTACATCGCTAGTGAACCACCAGGTTATGGGGAAGACAAAGAAAATATTCATTCCCCAGGAGTTGATTCAAATCATACTGTCAACAATGTAGAAGGCGCTACCGGATCAGCTTACGACTCAGGCCTTAGTTTCTCGGGTACTCTCCATGCTTTTGAAGATAGTGATGATGAAGATACACGATCGGTTAATTCTGAGTCCAGTGTCTCTGTGGGAAAATACCGTGTGAAAGGAAGTTTCTCTTCCATTCTCCAGTCAATCATGGAAAAATATGGTGACATAGCCTCAAGCTGTCAATTGGAATCAGTTGTCATGCGCTCTTACTATCTCGAGTGCGTATGTTATATAGTCCAAGAGTTGAAGCGAACTTCGGTTAAGCAGCTGACAAAGCCTAAAGTCAAAGAAATGTTATCTATGCTTAAGGATGTAGAATCTTCGGGAATGGATGTTGGTTGGCTCCGGTTTCTGATCAACGAGTGTGCAGAAGCCATTGAACTCATCACTCAGCATCGAGCATTCAAAGCAGCAAAGGCAAACTGCGACCGTGATATAGAATCAGCAAGGAAAGAACTTGAATCGCAAATGGAGGTTCTGGCGCTGAAAGAAAAGGAGGTTGCCGATGCCAAGAAGCAAGTCGCTGAAACGAGAGCCCACCTGAGGGAGCTCGAGCTTAAATCTTCTGTCTTGACCGAGAGTATTAGTAACATGAAGTCCAAGGTCGAAAATCTTCCAAGCAAACCTTTGTTGGACAAAGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

526

Amino Acids

58.24

Weight (kDa)

6.34

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 317 - 522 2e-65 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 845
AciI CCGC 3 cut(s) 220, 231, 558
AclWI GGATC 5 cut(s) 306, 419, 460, 563, 931
AcsI RAATTY 2 cut(s) 212, 768
AcuI CTGAAG 4 cut(s) 111, 492, 636, 723
AfaI GTAC 4 cut(s) 21, 158, 633, 958
AfiI CCNNNNNNNGG 7 cut(s) 293, 434, 578, 764, 845, 1473, 1474
AflII CTTAAG 1 cut(s) 1235
AgsI TTSAA 7 cut(s) 724, 888, 974, 1177, 1310, 1336, 1391
AjnI CCWGG 3 cut(s) 592, 838, 874
AluBI AGCT 9 cut(s) 141, 337, 478, 622, 929, 1107, 1198, 1480, 1486
AluI AGCT 9 cut(s) 141, 337, 478, 622, 929, 1107, 1198, 1480, 1486
Alw21I GWGCWC 1 cut(s) 1482
Alw26I GTCTC 2 cut(s) 9, 1027
AlwI GGATC 5 cut(s) 306, 419, 460, 563, 931
Ama87I CYCGRG 4 cut(s) 801, 952, 1145, 1481
AoxI GGCC 3 cut(s) 100, 590, 940
ApeKI GCWGC 7 cut(s) 135, 138, 233, 236, 698, 1195, 1340
ApoI RAATTY 2 cut(s) 212, 768
Asp700I GAANNNNTTC 2 cut(s) 864, 1542
AspLEI GCGC 3 cut(s) 917, 1134, 1414
AspS9I GGNCC 1 cut(s) 100
AsuHPI GGTGA 3 cut(s) 406, 683, 1103
AvaI CYCGRG 4 cut(s) 801, 952, 1145, 1481
AxyI CCTNAGG 1 cut(s) 1472
BanII GRGCYC 3 cut(s) 614, 1468, 1482
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 858
Bbv12I GWGCWC 1 cut(s) 1482
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 7 cut(s) 147, 150, 220, 223, 685, 1207, 1352
BccI CCATC 3 cut(s) 379, 532, 769
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 3 cut(s) 594, 840, 876
BclI TGATCA 1 cut(s) 1284
BcoDI GTCTC 2 cut(s) 9, 1027
BfaI CTAG 1 cut(s) 828
BfoI RGCGCY 2 cut(s) 918, 1415
BfrI CTTAAG 1 cut(s) 1235
BisI GCNGC 8 cut(s) 136, 139, 231, 234, 237, 699, 1196, 1341
BlsI GCNGC 8 cut(s) 137, 140, 232, 235, 238, 700, 1197, 1342
Bme1390I CCNGG 3 cut(s) 594, 840, 876
BmeT110I CYCGRG 4 cut(s) 801, 952, 1145, 1481
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 3 cut(s) 556, 598, 1274
BmrFI CCNGG 3 cut(s) 594, 840, 876
BmsI GCATC 5 cut(s) 197, 355, 703, 1333, 1426
BpiI GAAGAC 1 cut(s) 858
BplI GAGNNNNNCTC 4 cut(s) 395, 427, 539, 571
BpmI CTGGAG 1 cut(s) 1052
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 2 cut(s) 97, 1087
BsaBI GATNNNNATC 1 cut(s) 888
BsaJI CCNNGG 2 cut(s) 874, 1530
BsaWI WCCGGW 2 cut(s) 920, 1275
Bsc4I CCNNNNNNNGG 7 cut(s) 293, 434, 578, 764, 845, 1473, 1474
Bse1I ACTGG 3 cut(s) 496, 1017, 1069
Bse21I CCTNAGG 1 cut(s) 1472
Bse8I GATNNNNATC 1 cut(s) 888
BseBI CCWGG 3 cut(s) 594, 840, 876
BseDI CCNNGG 2 cut(s) 874, 1530
BseGI GGATG 2 cut(s) 1246, 1267
BseJI GATNNNNATC 1 cut(s) 888
BseLI CCNNNNNNNGG 7 cut(s) 293, 434, 578, 764, 845, 1473, 1474
BseMII CTCAG 7 cut(s) 165, 344, 470, 951, 1002, 1334, 1463
BseNI ACTGG 3 cut(s) 496, 1017, 1069
BseXI GCAGC 7 cut(s) 147, 150, 220, 223, 685, 1207, 1352
BsgI GTGCAG 1 cut(s) 1317
Bsh1285I CGRYCG 2 cut(s) 1001, 1360
BshFI GGCC 3 cut(s) 102, 592, 942
BsiEI CGRYCG 2 cut(s) 1001, 1360
BsiHKAI GWGCWC 1 cut(s) 1482
BsiHKCI CYCGRG 4 cut(s) 801, 952, 1145, 1481
BsiSI CCGG 3 cut(s) 103, 921, 1276
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 7 cut(s) 293, 434, 578, 764, 845, 1473, 1474
BsmAI GTCTC 2 cut(s) 9, 1027
BsmFI GGGAC 1 cut(s) 266
BsmI GAATGC 2 cut(s) 65, 1331
BsnI GGCC 3 cut(s) 102, 592, 942
BsoBI CYCGRG 4 cut(s) 801, 952, 1145, 1481
Bsp1286I GDGCHC 3 cut(s) 614, 1468, 1482
Bsp143I GATC 7 cut(s) 311, 424, 452, 568, 923, 998, 1284
BspACI CCGC 3 cut(s) 220, 231, 558
BspANI GGCC 3 cut(s) 102, 592, 942
BspCNI CTCAG 7 cut(s) 166, 343, 469, 950, 1003, 1333, 1464
BspLI GGNNCC 3 cut(s) 556, 598, 1274
BspPI GGATC 5 cut(s) 306, 419, 460, 563, 931
BspQI GCTCTTC 2 cut(s) 468, 612
BspTI CTTAAG 1 cut(s) 1235
BsrI ACTGG 3 cut(s) 496, 1017, 1069
BssECI CCNNGG 2 cut(s) 874, 1530
BssMI GATC 7 cut(s) 311, 424, 452, 568, 923, 998, 1284
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 1530
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 3 cut(s) 594, 840, 876
Bst4CI ACNGT 4 cut(s) 812, 898, 1040, 1361
Bst6I CTCTTC 4 cut(s) 10, 468, 612, 1063
BstAFI CTTAAG 1 cut(s) 1235
BstDEI CTNAG 9 cut(s) 174, 330, 384, 456, 937, 944, 1011, 1320, 1472
BstF5I GGATG 2 cut(s) 1246, 1267
BstH2I RGCGCY 2 cut(s) 918, 1415
BstHHI GCGC 3 cut(s) 917, 1134, 1414
BstKTI GATC 7 cut(s) 314, 427, 455, 571, 926, 1001, 1287
BstMAI GTCTC 2 cut(s) 9, 1027
BstMBI GATC 7 cut(s) 311, 424, 452, 568, 923, 998, 1284
BstMCI CGRYCG 2 cut(s) 1001, 1360
BstMWI GCNNNNNNNGC 4 cut(s) 236, 1204, 1337, 1346
BstNI CCWGG 3 cut(s) 594, 840, 876
BstSCI CCNGG 3 cut(s) 592, 838, 874
BstV1I GCAGC 7 cut(s) 147, 150, 220, 223, 685, 1207, 1352
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 2 cut(s) 311, 452
BstYI RGATCY 2 cut(s) 311, 452
Bsu36I CCTNAGG 1 cut(s) 1472
BsuRI GGCC 3 cut(s) 102, 592, 942
BtgZI GCGATG 1 cut(s) 808
BtsCI GGATG 2 cut(s) 1246, 1267
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 3 cut(s) 63, 272, 1024
CfoI GCGC 3 cut(s) 917, 1134, 1414
Cfr13I GGNCC 1 cut(s) 100
CsiI ACCWGGT 1 cut(s) 838
Csp6I GTAC 4 cut(s) 20, 157, 632, 957
CviAII CATG 6 cut(s) 56, 203, 965, 1078, 1129, 1522
CviQI GTAC 4 cut(s) 20, 157, 632, 957
DdeI CTNAG 9 cut(s) 174, 330, 384, 456, 937, 944, 1011, 1320, 1472
DpnI GATC 7 cut(s) 313, 426, 454, 570, 925, 1000, 1286
DpnII GATC 7 cut(s) 311, 424, 452, 568, 923, 998, 1284
DraI TTTAAA 1 cut(s) 145
Eam1104I CTCTTC 4 cut(s) 10, 468, 612, 1063
EarI CTCTTC 4 cut(s) 10, 468, 612, 1063
Ecl136II GAGCTC 1 cut(s) 1480
Eco130I CCWWGG 1 cut(s) 1530
Eco147I AGGCCT 2 cut(s) 592, 942
Eco24I GRGCYC 3 cut(s) 614, 1468, 1482
Eco53kI GAGCTC 1 cut(s) 1480
Eco57I CTGAAG 4 cut(s) 111, 492, 636, 723
Eco81I CCTNAGG 1 cut(s) 1472
Eco88I CYCGRG 4 cut(s) 801, 952, 1145, 1481
EcoICRI GAGCTC 1 cut(s) 1480
EcoRI GAATTC 2 cut(s) 212, 768
EcoRII CCWGG 3 cut(s) 592, 838, 874
EcoT14I CCWWGG 1 cut(s) 1530
EcoT38I GRGCYC 3 cut(s) 614, 1468, 1482
ErhI CCWWGG 1 cut(s) 1530
FaeI CATG 6 cut(s) 59, 206, 968, 1081, 1132, 1525
FalI AAGNNNNNCTT 4 cut(s) 1042, 1074, 1540, 1572
FaqI GGGAC 1 cut(s) 266
FatI CATG 6 cut(s) 55, 202, 964, 1077, 1128, 1521
FbaI TGATCA 1 cut(s) 1284
Fnu4HI GCNGC 8 cut(s) 136, 139, 231, 234, 237, 699, 1196, 1341
FokI GGATG 2 cut(s) 1253, 1274
FriOI GRGCYC 3 cut(s) 614, 1468, 1482
Fsp4HI GCNGC 8 cut(s) 136, 139, 231, 234, 237, 699, 1196, 1341
FspBI CTAG 1 cut(s) 828
GlaI GCGC 3 cut(s) 916, 1133, 1413
GluI GCNGC 8 cut(s) 136, 139, 231, 234, 237, 699, 1196, 1341
GsuI CTGGAG 1 cut(s) 1052
HaeII RGCGCY 2 cut(s) 918, 1415
HaeIII GGCC 3 cut(s) 102, 592, 942
HapII CCGG 3 cut(s) 103, 921, 1276
HhaI GCGC 3 cut(s) 917, 1134, 1414
Hin1II CATG 6 cut(s) 59, 206, 968, 1081, 1132, 1525
Hin6I GCGC 3 cut(s) 915, 1132, 1412
HinP1I GCGC 3 cut(s) 915, 1132, 1412
HincII GTYRAC 2 cut(s) 111, 901
HindII GTYRAC 2 cut(s) 111, 901
HinfI GANTC 9 cut(s) 263, 518, 884, 935, 1013, 1118, 1247, 1370, 1391
HpaII CCGG 3 cut(s) 103, 921, 1276
HphI GGTGA 3 cut(s) 406, 683, 1103
Hpy166II GTNNAC 6 cut(s) 22, 37, 111, 272, 833, 901
Hpy188I TCNGA 6 cut(s) 9, 127, 472, 517, 1012, 1284
Hpy188III TCNNGA 8 cut(s) 315, 332, 600, 765, 803, 1145, 1254, 1501
Hpy8I GTNNAC 6 cut(s) 22, 37, 111, 272, 833, 901
HpyAV CCTTC 5 cut(s) 86, 91, 582, 730, 905
HpyCH4III ACNGT 4 cut(s) 812, 898, 1040, 1361
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 3 cut(s) 63, 541, 1298
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 1204, 1337, 1346
HpyF3I CTNAG 9 cut(s) 174, 330, 384, 456, 937, 944, 1011, 1320, 1472
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 6 cut(s) 59, 206, 968, 1081, 1132, 1525
HspAI GCGC 3 cut(s) 915, 1132, 1412
Ksp22I TGATCA 1 cut(s) 1284
Kzo9I GATC 7 cut(s) 311, 424, 452, 568, 923, 998, 1284
LguI GCTCTTC 2 cut(s) 468, 612
LmnI GCTCC 6 cut(s) 334, 373, 560, 730, 1278, 1477
Lsp1109I GCAGC 7 cut(s) 147, 150, 220, 223, 685, 1207, 1352
LweI GCATC 5 cut(s) 197, 355, 703, 1333, 1426
MabI ACCWGGT 1 cut(s) 838
MaeI CTAG 1 cut(s) 828
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 3 cut(s) 487, 1091, 1517
MalI GATC 7 cut(s) 313, 426, 454, 570, 925, 1000, 1286
MboI GATC 7 cut(s) 311, 424, 452, 568, 923, 998, 1284
MfeI CAATTG 1 cut(s) 1112
MflI RGATCY 2 cut(s) 311, 452
MhlI GDGCHC 3 cut(s) 614, 1468, 1482
MluCI AATT 8 cut(s) 70, 212, 398, 504, 642, 768, 1006, 1112
MlyI GAGTC 2 cut(s) 929, 1022
MmeI TCCRAC 1 cut(s) 1542
MroXI GAANNNNTTC 2 cut(s) 864, 1542
MseI TTAA 8 cut(s) 144, 503, 645, 657, 1005, 1191, 1236, 1488
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 2 cut(s) 233, 1198
MspCI CTTAAG 1 cut(s) 1235
MspI CCGG 3 cut(s) 103, 921, 1276
MspR9I CCNGG 3 cut(s) 594, 840, 876
MunI CAATTG 1 cut(s) 1112
Mva1269I GAATGC 2 cut(s) 65, 1331
MvaI CCWGG 3 cut(s) 594, 840, 876
MwoI GCNNNNNNNGC 4 cut(s) 236, 1204, 1337, 1346
NdeII GATC 7 cut(s) 311, 424, 452, 568, 923, 998, 1284
NlaIII CATG 6 cut(s) 59, 206, 968, 1081, 1132, 1525
NlaIV GGNNCC 3 cut(s) 556, 598, 1274
NmuCI GTSAC 1 cut(s) 1091
PaeR7I CTCGAG 3 cut(s) 801, 1145, 1481
PceI AGGCCT 2 cut(s) 592, 942
PciSI GCTCTTC 2 cut(s) 468, 612
PctI GAATGC 2 cut(s) 65, 1331
PdmI GAANNNNTTC 2 cut(s) 864, 1542
PfeI GAWTC 7 cut(s) 263, 518, 884, 1118, 1247, 1370, 1391
PflFI GACNNNGTC 1 cut(s) 1568
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 8 cut(s) 137, 140, 232, 235, 238, 700, 1197, 1342
Ple19I CGATCG 1 cut(s) 1001
PleI GAGTC 2 cut(s) 929, 1021
PpsI GAGTC 2 cut(s) 929, 1021
Psp124BI GAGCTC 1 cut(s) 1482
Psp6I CCWGG 3 cut(s) 592, 838, 874
PspGI CCWGG 3 cut(s) 592, 838, 874
PspN4I GGNNCC 3 cut(s) 556, 598, 1274
PspPI GGNCC 1 cut(s) 100
PspXI VCTCGAGB 1 cut(s) 1481
PsuI RGATCY 2 cut(s) 311, 452
PsyI GACNNNGTC 1 cut(s) 1568
PvuI CGATCG 1 cut(s) 1001
PvuII CAGCTG 1 cut(s) 1198
RsaI GTAC 4 cut(s) 21, 158, 633, 958
RsaNI GTAC 4 cut(s) 20, 157, 632, 957
RseI CAYNNNNRTG 1 cut(s) 363
SacI GAGCTC 1 cut(s) 1482
SapI GCTCTTC 2 cut(s) 468, 612
SaqAI TTAA 8 cut(s) 144, 503, 645, 657, 1005, 1191, 1236, 1488
SatI GCNGC 8 cut(s) 136, 139, 231, 234, 237, 699, 1196, 1341
Sau3AI GATC 7 cut(s) 311, 424, 452, 568, 923, 998, 1284
Sau96I GGNCC 1 cut(s) 100
SchI GAGTC 2 cut(s) 929, 1022
ScrFI CCNGG 3 cut(s) 594, 840, 876
SduI GDGCHC 3 cut(s) 614, 1468, 1482
SexAI ACCWGGT 1 cut(s) 838
SfaNI GCATC 5 cut(s) 197, 355, 703, 1333, 1426
Sfr274I CTCGAG 3 cut(s) 801, 1145, 1481
SlaI CTCGAG 3 cut(s) 801, 1145, 1481
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 6 cut(s) 76, 801, 1102, 1145, 1235, 1481
SmoI CTYRAG 6 cut(s) 76, 801, 1102, 1145, 1235, 1481
Sse9I AATT 8 cut(s) 70, 212, 398, 504, 642, 768, 1006, 1112
SseBI AGGCCT 2 cut(s) 592, 942
SsiI CCGC 3 cut(s) 220, 231, 558
SspI AATATT 1 cut(s) 865
SspMI CTAG 1 cut(s) 828
SstI GAGCTC 1 cut(s) 1482
StuI AGGCCT 2 cut(s) 592, 942
StyD4I CCNGG 3 cut(s) 592, 838, 874
StyI CCWWGG 1 cut(s) 1530
TaaI ACNGT 4 cut(s) 812, 898, 1040, 1361
TaiI ACGT 1 cut(s) 42
TaqI TCGA 6 cut(s) 496, 802, 1146, 1327, 1482, 1536
TaqII GACCGA 2 cut(s) 357, 1520
TasI AATT 8 cut(s) 70, 212, 398, 504, 642, 768, 1006, 1112
TatI WGTACW 2 cut(s) 19, 156
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 7 cut(s) 263, 518, 884, 1118, 1247, 1370, 1391
Tru1I TTAA 8 cut(s) 144, 503, 645, 657, 1005, 1191, 1236, 1488
Tru9I TTAA 8 cut(s) 144, 503, 645, 657, 1005, 1191, 1236, 1488
TscAI CASTG 3 cut(s) 70, 279, 1024
TseFI GTSAC 1 cut(s) 1091
TseI GCWGC 7 cut(s) 135, 138, 233, 236, 698, 1195, 1340
Tsp45I GTSAC 1 cut(s) 1091
TspDTI ATGAA 5 cut(s) 72, 654, 857, 1002, 1538
TspGWI ACGGA 2 cut(s) 369, 729
TspRI CASTG 3 cut(s) 70, 279, 1024
Tth111I GACNNNGTC 1 cut(s) 1568
Van91I CCANNNNNTGG 1 cut(s) 845
Vha464I CTTAAG 1 cut(s) 1235
XapI RAATTY 2 cut(s) 212, 768
XcmI CCANNNNNNNNNTGG 1 cut(s) 1024
XhoI CTCGAG 3 cut(s) 801, 1145, 1481
XmnI GAANNNNTTC 2 cut(s) 864, 1542
XspI CTAG 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.