MD05G1122800.v1.1

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002091
Physical Location & Seq
Reverse (-)
24548782 .. 24553034
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1122800.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGTCTCTGATCGGAAAAATACACCCCGAATGTGTTAATGTAGTCAATCCCTACCATGAATGCACTGAAATTTGCCTGAGAAAGATTGCTGAAGGGAAAGGCCGAAAGGTTACGAAAAAGAAATCAGATATTGGCAGCAGTTTTAAAGGTGGTGAGTACAAAAAAAGGGGGGCTGAAGAGAAAAGAGCATCCAATCCTTACCATGACCCTGAATTCTTCCGCCAAAGAGCCGTTGGTGCCGAACCTCGTGGGGGCAAAAAAGAATCAGGTTCAATAGTTGATATTCCTATATTGGGCAAACAAAGGGAGGGATCCCGACCAAGGACCCCTCAGGAGGTTGTCCGAAGTGTGATTTATCATTTAGATGCTCAGTCATCTCCATCTAAGGAAAATGTGAAATTAGAGAACGGCTCTGTCCTTGATGATCCTATATTCGGCAGAAGGAAGCAGGGAACTCAGCCAAAGCCCTCTGAAGAGCTTAAGAATGCAACTGGTCGAGGTGTAATTTATCCCTCTGATTCTCGATCTGAAGAAATAGTGCAAGTAGAAAATGGCTCCGTGCTTGATGATCCTATATTTGGCAGACAAAGGCCTGGTTCCAGACCAATGCGCCCTGAAGAGCTTGACAATGCATCCGGTGAAATTAAGGAAGAGGTTAAAATAGGGAATGGTGAGCATAAATCTTATTCTCCACCGTCTGCTGAAGTAAAAGATGCTTCATTCAACAAGGATCCAGTTCCGTCTTCTCTGTTAGTGCCACCATCTGGAATTATCACAATGCCTGAAAATCCCAAACACCCTCGAGAGAATGGTGCTACTTACATCGCTAGTGAACCACCCAGTAATGGGGAAGACAAGGAAAATATTAATCATTCACCTGGAGTTGATTCAAATCATACCGTCAGCAATGTAGGAGAAGGCTCTACTGGATCAGCTTTCGACTCAAGATTTAGTTTCTCAGGTACTGTCCATGCTTTTGAAGATAGTGATGATGACGATACACGATCTGTTATTTCTGATGCCAGTGTCTCTGTGGGAAAATACCGTGTGAAAGGAAGTTTCTCTTCCATTCTCCAGTCAATCATGGAAAAATATGGTGATATAGCCGCAAGCTGTCAATTGGAATCAGTTGCCATGCGCTCTTATTATCTCGAGTGTGTATGTTATATAGTCCAAGAGTTGAAGCGAACTTCGATTAAGGAGCTGACAAAGCCTAGAGTCAAACAAATGTTATCTATGCTTAAGGATGTAGAATCTTCGGGAATGGATGTTGGTTGGCTCCGCTGTCTGATCAACAAATATGAAGAATCCATTGAACTCGTCACTCAGCATCGAGCATTCGAAGTAGCAAAGGAAAACTGCGACCGTGATTTAGAATCCACAAGGAAAGAACTTGAACCGCAAATTGAGGCTCTGGTTCTGAAAGAAAAGGAGGTTGCCAATGCCAAGAAGCAAGTTGCTGAAATGAAAGCCCACCTGCGGGAGCTCGAGCTTAAATCTTCCGTCTTGAGCGACAGTGTTAGTAACATGAAGTCCAAGGTCGAAAATCTTCCTAGTAAACCGTTGTTGGACAAAGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

528

Amino Acids

58.3

Weight (kDa)

6.34

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 297 - 524 2.2e-64 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1485
Acc36I ACCTGC 1 cut(s) 1485
AccB1I GGYRCC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 764
AciI CCGC 5 cut(s) 220, 1107, 1282, 1400, 1480
AclWI GGATC 7 cut(s) 306, 319, 419, 563, 725, 738, 937
AcsI RAATTY 2 cut(s) 69, 212
AcuI CTGAAG 6 cut(s) 111, 195, 492, 549, 636, 723
AfaI GTAC 2 cut(s) 158, 964
AflII CTTAAG 2 cut(s) 479, 1241
AgsI TTSAA 7 cut(s) 273, 724, 891, 980, 1183, 1316, 1397
AjnI CCWGG 2 cut(s) 592, 877
AluBI AGCT 7 cut(s) 478, 622, 935, 1113, 1204, 1486, 1492
AluI AGCT 7 cut(s) 478, 622, 935, 1113, 1204, 1486, 1492
Alw21I GWGCWC 1 cut(s) 1488
Alw26I GTCTC 2 cut(s) 9, 1033
AlwI GGATC 7 cut(s) 306, 319, 419, 563, 725, 738, 937
AlwNI CAGNNNCTG 1 cut(s) 965
Ama87I CYCGRG 3 cut(s) 801, 1151, 1487
AoxI GGCC 2 cut(s) 100, 590
ApeKI GCWGC 1 cut(s) 135
ApoI RAATTY 2 cut(s) 69, 212
AseI ATTAAT 1 cut(s) 867
Asp700I GAANNNNTTC 1 cut(s) 1548
AspLEI GCGC 2 cut(s) 612, 1140
AspS9I GGNCC 1 cut(s) 324
AsuHPI GGTGA 5 cut(s) 164, 650, 683, 867, 1109
AsuII TTCGAA 1 cut(s) 1341
AvaI CYCGRG 3 cut(s) 801, 1151, 1487
AvaII GGWCC 1 cut(s) 324
AxyI CCTNAGG 1 cut(s) 330
BamHI GGATCC 2 cut(s) 311, 730
BanI GGYRCC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 1488
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 2 cut(s) 735, 858
Bbv12I GWGCWC 1 cut(s) 1488
BbvI GCAGC 1 cut(s) 147
BccI CCATC 2 cut(s) 388, 769
BceAI ACGGC 2 cut(s) 215, 424
BciT130I CCWGG 2 cut(s) 594, 879
BclI TGATCA 1 cut(s) 1290
BcoDI GTCTC 2 cut(s) 9, 1033
BfaI CTAG 3 cut(s) 828, 1215, 1554
BfrI CTTAAG 2 cut(s) 479, 1241
BfuAI ACCTGC 1 cut(s) 1485
BisI GCNGC 2 cut(s) 136, 1107
BlsI GCNGC 2 cut(s) 137, 1108
Bme1390I CCNGG 2 cut(s) 594, 879
Bme18I GGWCC 1 cut(s) 324
BmeT110I CYCGRG 3 cut(s) 801, 1151, 1487
BmgT120I GGNCC 1 cut(s) 324
BmiI GGNNCC 7 cut(s) 238, 313, 326, 556, 598, 732, 1280
BmrFI CCNGG 2 cut(s) 594, 879
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 6 cut(s) 197, 355, 641, 703, 1009, 1339
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 2 cut(s) 735, 858
BplI GAGNNNNNCTC 2 cut(s) 395, 427
BpmI CTGGAG 2 cut(s) 900, 1058
Bpu14I TTCGAA 1 cut(s) 1341
BpuEI CTTGAG 2 cut(s) 928, 1528
BsaBI GATNNNNATC 1 cut(s) 891
BsaJI CCNNGG 2 cut(s) 320, 1536
BsaWI WCCGGW 1 cut(s) 635
Bse1I ACTGG 6 cut(s) 496, 734, 840, 931, 1023, 1075
Bse21I CCTNAGG 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 913
Bse8I GATNNNNATC 1 cut(s) 891
BseBI CCWGG 2 cut(s) 594, 879
BseDI CCNNGG 2 cut(s) 320, 1536
BseGI GGATG 4 cut(s) 188, 632, 1252, 1273
BseJI GATNNNNATC 1 cut(s) 891
BseMI GCAATG 1 cut(s) 913
BseMII CTCAG 6 cut(s) 68, 344, 383, 470, 972, 1340
BseNI ACTGG 6 cut(s) 496, 734, 840, 931, 1023, 1075
BseXI GCAGC 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 1366
BshFI GGCC 2 cut(s) 102, 592
BshNI GGYRCC 1 cut(s) 236
BsiEI CGRYCG 1 cut(s) 1366
BsiHKAI GWGCWC 1 cut(s) 1488
BsiHKCI CYCGRG 3 cut(s) 801, 1151, 1487
BsiSI CCGG 1 cut(s) 636
BsmAI GTCTC 2 cut(s) 9, 1033
BsmI GAATGC 3 cut(s) 65, 490, 1337
BsnI GGCC 2 cut(s) 102, 592
BsoBI CYCGRG 3 cut(s) 801, 1151, 1487
Bsp119I TTCGAA 1 cut(s) 1341
Bsp1286I GDGCHC 1 cut(s) 1488
Bsp143I GATC 9 cut(s) 9, 311, 424, 524, 568, 730, 929, 1004, 1290
BspACI CCGC 5 cut(s) 220, 1107, 1282, 1400, 1480
BspANI GGCC 2 cut(s) 102, 592
BspCNI CTCAG 6 cut(s) 69, 343, 382, 469, 971, 1339
BspLI GGNNCC 7 cut(s) 238, 313, 326, 556, 598, 732, 1280
BspMI ACCTGC 1 cut(s) 1485
BspPI GGATC 7 cut(s) 306, 319, 419, 563, 725, 738, 937
BspQI GCTCTTC 2 cut(s) 468, 612
BspT104I TTCGAA 1 cut(s) 1341
BspT107I GGYRCC 1 cut(s) 236
BspTI CTTAAG 2 cut(s) 479, 1241
BsrDI GCAATG 1 cut(s) 913
BsrI ACTGG 6 cut(s) 496, 734, 840, 931, 1023, 1075
BssECI CCNNGG 2 cut(s) 320, 1536
BssMI GATC 9 cut(s) 9, 311, 424, 524, 568, 730, 929, 1004, 1290
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 2 cut(s) 320, 1536
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 2 cut(s) 594, 879
Bst4CI ACNGT 7 cut(s) 696, 901, 967, 1046, 1367, 1517, 1563
Bst6I CTCTTC 5 cut(s) 171, 468, 612, 645, 1069
BstAFI CTTAAG 2 cut(s) 479, 1241
BstBI TTCGAA 1 cut(s) 1341
BstC8I GCNNGC 1 cut(s) 1111
BstDEI CTNAG 7 cut(s) 77, 330, 369, 384, 456, 958, 1326
BstF5I GGATG 4 cut(s) 188, 632, 1252, 1273
BstHHI GCGC 2 cut(s) 612, 1140
BstKTI GATC 9 cut(s) 12, 314, 427, 527, 571, 733, 932, 1007, 1293
BstMAI GTCTC 2 cut(s) 9, 1033
BstMBI GATC 9 cut(s) 9, 311, 424, 524, 568, 730, 929, 1004, 1290
BstMCI CGRYCG 1 cut(s) 1366
BstMWI GCNNNNNNNGC 2 cut(s) 236, 1210
BstNI CCWGG 2 cut(s) 594, 879
BstSCI CCNGG 2 cut(s) 592, 877
BstV1I GCAGC 1 cut(s) 147
BstV2I GAAGAC 2 cut(s) 735, 858
BstX2I RGATCY 2 cut(s) 311, 730
BstYI RGATCY 2 cut(s) 311, 730
Bsu36I CCTNAGG 1 cut(s) 330
BsuRI GGCC 2 cut(s) 102, 592
BtgZI GCGATG 1 cut(s) 808
BtsCI GGATG 4 cut(s) 188, 632, 1252, 1273
BtsIMutI CAGTG 3 cut(s) 63, 1030, 1522
BveI ACCTGC 1 cut(s) 1485
Cac8I GCNNGC 1 cut(s) 1111
CaiI CAGNNNCTG 1 cut(s) 965
CfoI GCGC 2 cut(s) 612, 1140
Cfr13I GGNCC 1 cut(s) 324
Csp6I GTAC 2 cut(s) 157, 963
CviAII CATG 6 cut(s) 56, 203, 971, 1084, 1135, 1528
CviQI GTAC 2 cut(s) 157, 963
DdeI CTNAG 7 cut(s) 77, 330, 369, 384, 456, 958, 1326
DpnI GATC 9 cut(s) 11, 313, 426, 526, 570, 732, 931, 1006, 1292
DpnII GATC 9 cut(s) 9, 311, 424, 524, 568, 730, 929, 1004, 1290
DraI TTTAAA 1 cut(s) 145
Eam1104I CTCTTC 5 cut(s) 171, 468, 612, 645, 1069
EarI CTCTTC 5 cut(s) 171, 468, 612, 645, 1069
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 1486
Eco130I CCWWGG 2 cut(s) 320, 1536
Eco147I AGGCCT 1 cut(s) 592
Eco24I GRGCYC 1 cut(s) 1488
Eco47I GGWCC 1 cut(s) 324
Eco53kI GAGCTC 1 cut(s) 1486
Eco57I CTGAAG 6 cut(s) 111, 195, 492, 549, 636, 723
Eco81I CCTNAGG 1 cut(s) 330
Eco88I CYCGRG 3 cut(s) 801, 1151, 1487
EcoICRI GAGCTC 1 cut(s) 1486
EcoO109I RGGNCCY 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 592, 877
EcoT14I CCWWGG 2 cut(s) 320, 1536
EcoT22I ATGCAT 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 1488
ErhI CCWWGG 2 cut(s) 320, 1536
FaeI CATG 6 cut(s) 59, 206, 974, 1087, 1138, 1531
FalI AAGNNNNNCTT 2 cut(s) 1048, 1080
FatI CATG 6 cut(s) 55, 202, 970, 1083, 1134, 1527
FauI CCCGC 1 cut(s) 1473
FbaI TGATCA 1 cut(s) 1290
Fnu4HI GCNGC 2 cut(s) 136, 1107
FokI GGATG 4 cut(s) 175, 619, 1259, 1280
FriOI GRGCYC 1 cut(s) 1488
Fsp4HI GCNGC 2 cut(s) 136, 1107
FspBI CTAG 3 cut(s) 828, 1215, 1554
GlaI GCGC 2 cut(s) 611, 1139
GluI GCNGC 2 cut(s) 136, 1107
GsuI CTGGAG 2 cut(s) 900, 1058
HaeIII GGCC 2 cut(s) 102, 592
HapII CCGG 1 cut(s) 636
HhaI GCGC 2 cut(s) 612, 1140
Hin1II CATG 6 cut(s) 59, 206, 974, 1087, 1138, 1531
Hin6I GCGC 2 cut(s) 610, 1138
HinP1I GCGC 2 cut(s) 610, 1138
HinfI GANTC 9 cut(s) 263, 518, 887, 941, 1124, 1218, 1253, 1307, 1376
HpaII CCGG 1 cut(s) 636
HphI GGTGA 5 cut(s) 164, 650, 683, 867, 1109
Hpy166II GTNNAC 2 cut(s) 833, 1559
Hpy8I GTNNAC 2 cut(s) 833, 1559
HpyAV CCTTC 3 cut(s) 86, 435, 911
HpyCH4III ACNGT 7 cut(s) 696, 901, 967, 1046, 1367, 1517, 1563
HpyCH4V TGCA 4 cut(s) 63, 488, 541, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 1210
HpyF3I CTNAG 7 cut(s) 77, 330, 369, 384, 456, 958, 1326
Hsp92II CATG 6 cut(s) 59, 206, 974, 1087, 1138, 1531
HspAI GCGC 2 cut(s) 610, 1138
Ksp22I TGATCA 1 cut(s) 1290
Kzo9I GATC 9 cut(s) 9, 311, 424, 524, 568, 730, 929, 1004, 1290
LguI GCTCTTC 2 cut(s) 468, 612
LmnI GCTCC 4 cut(s) 560, 1201, 1284, 1483
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 6 cut(s) 197, 355, 641, 703, 1009, 1339
MaeI CTAG 3 cut(s) 828, 1215, 1554
MaeIII GTNAC 3 cut(s) 109, 1321, 1523
MalI GATC 9 cut(s) 11, 313, 426, 526, 570, 732, 931, 1006, 1292
MboI GATC 9 cut(s) 9, 311, 424, 524, 568, 730, 929, 1004, 1290
MfeI CAATTG 1 cut(s) 1118
MflI RGATCY 2 cut(s) 311, 730
MhlI GDGCHC 1 cut(s) 1488
MluCI AATT 8 cut(s) 69, 212, 398, 504, 642, 768, 1118, 1404
MlyI GAGTC 2 cut(s) 935, 1227
MmeI TCCRAC 1 cut(s) 1548
Mph1103I ATGCAT 1 cut(s) 634
MroXI GAANNNNTTC 1 cut(s) 1548
MseI TTAA 9 cut(s) 36, 144, 480, 645, 657, 867, 1197, 1242, 1494
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 1284
MspCI CTTAAG 2 cut(s) 479, 1241
MspI CCGG 1 cut(s) 636
MspR9I CCNGG 2 cut(s) 594, 879
MunI CAATTG 1 cut(s) 1118
Mva1269I GAATGC 3 cut(s) 65, 490, 1337
MvaI CCWGG 2 cut(s) 594, 879
MwoI GCNNNNNNNGC 2 cut(s) 236, 1210
NdeII GATC 9 cut(s) 9, 311, 424, 524, 568, 730, 929, 1004, 1290
NlaIII CATG 6 cut(s) 59, 206, 974, 1087, 1138, 1531
NlaIV GGNNCC 7 cut(s) 238, 313, 326, 556, 598, 732, 1280
NmuCI GTSAC 1 cut(s) 1321
NsiI ATGCAT 1 cut(s) 634
NspV TTCGAA 1 cut(s) 1341
PaeR7I CTCGAG 3 cut(s) 801, 1151, 1487
PaqCI CACCTGC 1 cut(s) 1485
PceI AGGCCT 1 cut(s) 592
PciSI GCTCTTC 2 cut(s) 468, 612
PctI GAATGC 3 cut(s) 65, 490, 1337
PdmI GAANNNNTTC 1 cut(s) 1548
PfeI GAWTC 7 cut(s) 263, 518, 887, 1124, 1253, 1307, 1376
PflFI GACNNNGTC 1 cut(s) 1574
PflMI CCANNNNNTGG 1 cut(s) 764
PkrI GCNGC 2 cut(s) 137, 1108
PleI GAGTC 2 cut(s) 935, 1226
PpsI GAGTC 2 cut(s) 935, 1226
PpuMI RGGWCCY 1 cut(s) 324
PshBI ATTAAT 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 1488
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 592, 877
PspGI CCWGG 2 cut(s) 592, 877
PspN4I GGNNCC 7 cut(s) 238, 313, 326, 556, 598, 732, 1280
PspPI GGNCC 1 cut(s) 324
PspPPI RGGWCCY 1 cut(s) 324
PspXI VCTCGAGB 1 cut(s) 1487
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 2 cut(s) 311, 730
PsyI GACNNNGTC 1 cut(s) 1574
RsaI GTAC 2 cut(s) 158, 964
RsaNI GTAC 2 cut(s) 157, 963
RseI CAYNNNNRTG 1 cut(s) 363
SacI GAGCTC 1 cut(s) 1488
SapI GCTCTTC 2 cut(s) 468, 612
SaqAI TTAA 9 cut(s) 36, 144, 480, 645, 657, 867, 1197, 1242, 1494
SatI GCNGC 2 cut(s) 136, 1107
Sau3AI GATC 9 cut(s) 9, 311, 424, 524, 568, 730, 929, 1004, 1290
Sau96I GGNCC 1 cut(s) 324
SchI GAGTC 2 cut(s) 935, 1227
ScrFI CCNGG 2 cut(s) 594, 879
SduI GDGCHC 1 cut(s) 1488
SfaNI GCATC 6 cut(s) 197, 355, 641, 703, 1009, 1339
Sfr274I CTCGAG 3 cut(s) 801, 1151, 1487
SfuI TTCGAA 1 cut(s) 1341
SinI GGWCC 1 cut(s) 324
SlaI CTCGAG 3 cut(s) 801, 1151, 1487
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 7 cut(s) 479, 801, 943, 1151, 1241, 1487, 1507
SmoI CTYRAG 7 cut(s) 479, 801, 943, 1151, 1241, 1487, 1507
Sse9I AATT 8 cut(s) 69, 212, 398, 504, 642, 768, 1118, 1404
SseBI AGGCCT 1 cut(s) 592
SsiI CCGC 5 cut(s) 220, 1107, 1282, 1400, 1480
SspI AATATT 1 cut(s) 865
SspMI CTAG 3 cut(s) 828, 1215, 1554
SstI GAGCTC 1 cut(s) 1488
StuI AGGCCT 1 cut(s) 592
StyD4I CCNGG 2 cut(s) 592, 877
StyI CCWWGG 2 cut(s) 320, 1536
TaaI ACNGT 7 cut(s) 696, 901, 967, 1046, 1367, 1517, 1563
TasI AATT 8 cut(s) 69, 212, 398, 504, 642, 768, 1118, 1404
TatI WGTACW 1 cut(s) 156
TauI GCSGC 1 cut(s) 1109
TfiI GAWTC 7 cut(s) 263, 518, 887, 1124, 1253, 1307, 1376
Tru1I TTAA 9 cut(s) 36, 144, 480, 645, 657, 867, 1197, 1242, 1494
Tru9I TTAA 9 cut(s) 36, 144, 480, 645, 657, 867, 1197, 1242, 1494
TscAI CASTG 3 cut(s) 70, 1030, 1522
TseFI GTSAC 1 cut(s) 1321
TseI GCWGC 1 cut(s) 135
Tsp45I GTSAC 1 cut(s) 1321
TspDTI ATGAA 5 cut(s) 72, 708, 1317, 1481, 1544
TspGWI ACGGA 3 cut(s) 547, 729, 1492
TspRI CASTG 3 cut(s) 70, 1030, 1522
Tth111I GACNNNGTC 1 cut(s) 1574
Van91I CCANNNNNTGG 1 cut(s) 764
Vha464I CTTAAG 2 cut(s) 479, 1241
VpaK11BI GGWCC 1 cut(s) 324
VspI ATTAAT 1 cut(s) 867
XapI RAATTY 2 cut(s) 69, 212
XcmI CCANNNNNNNNNTGG 2 cut(s) 230, 1030
XhoI CTCGAG 3 cut(s) 801, 1151, 1487
XmnI GAANNNNTTC 1 cut(s) 1548
XspI CTAG 3 cut(s) 828, 1215, 1554
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.