Rh6DG224800

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002091
Physical Location & Seq
Reverse (-)
40286536 .. 40290738
4203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG224800.1

Sequence Viewer

Length: 1647 bp
ATGTATTGTACCAAAAACCTACAGTACTTCCCACCAAGTCCAAGGACACACTATGAATTCCTAGTCAGTGTGGTAAGAAAAACAGAGATTAGTTTCTTTCATGCCATAGGAATGGCTGAGGTTCTGGATGGTAATATTCTTCTTTCATTGGCACATGATGCATTGGAGTTCCTGGGAAGAGAAGCCAATAAAGATTGTGGATTTATTTATTCATTCCTCTTTCACACTGGCTCACACATAATATGTATGGATTTTAATAATTCCTACCTTTTTTCAATGGCTATTTTAATTGCAGGCTCCATACTTGATATTCCTATATTGGGTGGACGAAAAGAGAGATCCCGACCAAAGACTCCTCAAGATCTTGACAATGTATCTGACCGAAGTGTGGTTTATCCTTATGATGCTCAATCATCTCCATCTAAGGACAAGGTGAATGTAGAGAATGGGTCTGTCCTTGATGATCCTATATTTGGAAAAAGAAAGCCAGTCTCCCAAACAAGGTCTGCTGAAGAGCATGAAAATGGTCAAGGTGTTGATCCTTCATCATTTCCAGAAAAGCTGAAAATAGAGAACGGATCTGCCCTTGATGATCCTATATTTGGCAGAAGAAAGCAGGTAGCCCAGCCAAAGCCCCATGAAGAGCTTGACAATGGATCCGGTCAAGGTTCAGTTAATCCTTCTGGTTCTCCCATGTCTCCATCTAATGAAATTGTGAAAATAGGGAATGGCTCCACCCTTGATGATCTTATATTTGGCCGCAAAAAGCAGGGATCTGAACCAAAGCCTGAAGAAGAGATTGAGAATGTACCTGTTGTGAAATTAGATAATGATGAGTATAAGTCTTATTCTGGACCGCTTGCTCAAGTAAAAGATCCTTCCTTGAACAAGAAGCAAATCAATTCCTCTGGGGCAGTGCCTACATCTGGAATTATCAAAATGACCCCCTCTCTTTCTCTCCCTACACACAATGGTGGAGAAGGTCCCACTGCCACACCTTCTGAAAACAGAAGCTTCAGTTTCTCAGGTTCTCCCTATGTTGAAGAGAGTGATGATGACGATGCACGATCTGTCAATTCTGATTCCAGTGTTTCTGTGGGAAAGTACCGTGTGAAAGGAAGCTTATGTTCCATTCTCCAGTCGATCTTTGACAAGTATGGTGATATAGCAGCAAGCTGTCAATTGGAATCAATTGTCATTCGCTCTTATTACCTCGAGTGTGTCTGTTATGTGGTTCAAGAGTTGAAACGCACTTCGGTGAAGCAGCTGACAAAGTCAAAAGTCAAAGAAATGTCAGCTATGCTCAAGGATGTAGAATCTTCAGGAATGGATGTTGGTTGGCTTCGTCTTATGCTCAATGAATGTGCGCAATCCATTGATCTCGTGAGTCAGCATCGAGCATTCGAACTAGAAAAGGCCAACTGTGATCGTGGCATTGAATCAATAAGGAAAGAACTAGAGTCCCAGATGGAGGCTTTGGCTCTGAAAGAAAAGGAGGTTGCTGATGCCAAGAAGCAAGTTTCTGAAACCAGAGCCCATCTGAGGGAGCTTGAGCTCAAATCATCTGTTTTGAGTGAGACTGTTAGTAATCTGAAGTCCAAGGTTGAAAATCTTCAAAGCAAATCATTGTTGGACAAGGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

548

Amino Acids

60.41

Weight (kDa)

5.52

Isoelectric Point (pI)

54.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 352 - 545 1.3e-64 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1368
Acc36I ACCTGC 1 cut(s) 607
AciI CCGC 2 cut(s) 760, 857
AclWI GGATC 9 cut(s) 333, 458, 533, 586, 587, 651, 664, 781, 869
AcoI YGGCCR 1 cut(s) 757
AcsI RAATTY 1 cut(s) 56
AcuI CTGAAG 5 cut(s) 531, 810, 1000, 1305, 1613
AfaI GTAC 4 cut(s) 10, 26, 810, 1106
AfiI CCNNNNNNNGG 9 cut(s) 320, 388, 473, 501, 602, 926, 1471, 1542, 1543
AgsI TTSAA 8 cut(s) 276, 886, 1043, 1238, 1246, 1439, 1607, 1616
AjnI CCWGG 1 cut(s) 171
AleI CACNNNNGTG 2 cut(s) 972, 1256
AluBI AGCT 9 cut(s) 562, 646, 1014, 1122, 1176, 1267, 1298, 1549, 1555
AluI AGCT 9 cut(s) 562, 646, 1014, 1122, 1176, 1267, 1298, 1549, 1555
Alw21I GWGCWC 1 cut(s) 1557
Alw26I GTCTC 3 cut(s) 496, 702, 1571
AlwI GGATC 9 cut(s) 333, 458, 533, 586, 587, 651, 664, 781, 869
Ama87I CYCGRG 1 cut(s) 1214
AoxI GGCC 2 cut(s) 757, 1416
ApeKI GCWGC 2 cut(s) 1169, 1264
ApoI RAATTY 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 1611
AspLEI GCGC 1 cut(s) 1369
AspS9I GGNCC 2 cut(s) 854, 983
AsuHPI GGTGA 3 cut(s) 445, 1172, 1270
AsuII TTCGAA 1 cut(s) 1404
AvaI CYCGRG 1 cut(s) 1214
AvaII GGWCC 2 cut(s) 854, 983
BamHI GGATCC 1 cut(s) 656
BanII GRGCYC 2 cut(s) 1537, 1557
BauI CACGAG 1 cut(s) 1382
Bbv12I GWGCWC 1 cut(s) 1557
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 1181, 1276
BccI CCATC 5 cut(s) 122, 427, 709, 1462, 1545
BciT130I CCWGG 1 cut(s) 173
BcoDI GTCTC 3 cut(s) 496, 702, 1571
BfaI CTAG 3 cut(s) 62, 1409, 1457
BfmI CTRYAG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 607
BglII AGATCT 1 cut(s) 361
BisI GCNGC 3 cut(s) 760, 1170, 1265
BlsI GCNGC 3 cut(s) 761, 1171, 1266
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 173
Bme18I GGWCC 2 cut(s) 854, 983
BmeT110I CYCGRG 1 cut(s) 1214
BmgT120I GGNCC 2 cut(s) 854, 983
BmiI GGNNCC 4 cut(s) 298, 658, 733, 985
BmrFI CCNGG 1 cut(s) 173
BmsI GCATC 5 cut(s) 148, 394, 1051, 1402, 1495
BpmI CTGGAG 1 cut(s) 1121
Bpu10I CCTNAGC 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 1404
BpuEI CTTGAG 4 cut(s) 342, 849, 1289, 1571
BsaJI CCNNGG 3 cut(s) 41, 172, 1599
BsaWI WCCGGW 1 cut(s) 659
Bsc4I CCNNNNNNNGG 9 cut(s) 320, 388, 473, 501, 602, 926, 1471, 1542, 1543
Bse1I ACTGG 4 cut(s) 232, 488, 1086, 1138
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 3 cut(s) 41, 172, 1599
BseGI GGATG 3 cut(s) 133, 1315, 1336
BseLI CCNNNNNNNGG 9 cut(s) 320, 388, 473, 501, 602, 926, 1471, 1542, 1543
BseMII CTCAG 3 cut(s) 108, 1038, 1532
BseNI ACTGG 4 cut(s) 232, 488, 1086, 1138
BseRI GAGGAG 1 cut(s) 345
BseXI GCAGC 2 cut(s) 1181, 1276
BseYI CCCAGC 1 cut(s) 624
BshFI GGCC 2 cut(s) 759, 1418
BsiHKAI GWGCWC 1 cut(s) 1557
BsiHKCI CYCGRG 1 cut(s) 1214
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 2 cut(s) 969, 1447
BslI CCNNNNNNNGG 9 cut(s) 320, 388, 473, 501, 602, 926, 1471, 1542, 1543
BsmAI GTCTC 3 cut(s) 496, 702, 1571
BsmFI GGGAC 2 cut(s) 969, 1447
BsmI GAATGC 1 cut(s) 1400
BsnI GGCC 2 cut(s) 759, 1418
BsoBI CYCGRG 1 cut(s) 1214
Bsp119I TTCGAA 1 cut(s) 1404
Bsp1286I GDGCHC 2 cut(s) 1537, 1557
BspACI CCGC 2 cut(s) 760, 857
BspANI GGCC 2 cut(s) 759, 1418
BspCNI CTCAG 3 cut(s) 109, 1037, 1533
BspLI GGNNCC 4 cut(s) 298, 658, 733, 985
BspMI ACCTGC 1 cut(s) 607
BspPI GGATC 9 cut(s) 333, 458, 533, 586, 587, 651, 664, 781, 869
BspQI GCTCTTC 2 cut(s) 507, 636
BspT104I TTCGAA 1 cut(s) 1404
BsrI ACTGG 4 cut(s) 232, 488, 1086, 1138
BssECI CCNNGG 3 cut(s) 41, 172, 1599
BssSI CACGAG 1 cut(s) 1382
BssT1I CCWWGG 2 cut(s) 41, 1599
Bst2BI CACGAG 1 cut(s) 1382
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 4 cut(s) 24, 1109, 1424, 1582
Bst6I CTCTTC 5 cut(s) 172, 507, 636, 789, 1038
BstAPI GCANNNNNTGC 1 cut(s) 158
BstBI TTCGAA 1 cut(s) 1404
BstC8I GCNNGC 3 cut(s) 295, 861, 1174
BstDEI CTNAG 4 cut(s) 117, 423, 1024, 1541
BstF5I GGATG 3 cut(s) 133, 1315, 1336
BstHHI GCGC 1 cut(s) 1369
BstMAI GTCTC 3 cut(s) 496, 702, 1571
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 1 cut(s) 171
BstSFI CTRYAG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 1181, 1276
BstX2I RGATCY 6 cut(s) 338, 361, 578, 656, 773, 874
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 6 cut(s) 338, 361, 578, 656, 773, 874
BsuRI GGCC 2 cut(s) 759, 1418
BtsCI GGATG 3 cut(s) 133, 1315, 1336
BtsI GCAGTG 2 cut(s) 921, 987
BtsIMutI CAGTG 5 cut(s) 73, 225, 921, 987, 1093
BveI ACCTGC 1 cut(s) 607
Cac8I GCNNGC 3 cut(s) 295, 861, 1174
CfoI GCGC 1 cut(s) 1369
Cfr13I GGNCC 2 cut(s) 854, 983
Csp6I GTAC 4 cut(s) 9, 25, 809, 1105
CviAII CATG 5 cut(s) 101, 155, 518, 638, 694
CviQI GTAC 4 cut(s) 9, 25, 809, 1105
DdeI CTNAG 4 cut(s) 117, 423, 1024, 1541
EaeI YGGCCR 1 cut(s) 757
Eam1104I CTCTTC 5 cut(s) 172, 507, 636, 789, 1038
EarI CTCTTC 5 cut(s) 172, 507, 636, 789, 1038
Ecl136II GAGCTC 1 cut(s) 1555
Eco130I CCWWGG 2 cut(s) 41, 1599
Eco24I GRGCYC 2 cut(s) 1537, 1557
Eco47I GGWCC 2 cut(s) 854, 983
Eco53kI GAGCTC 1 cut(s) 1555
Eco57I CTGAAG 5 cut(s) 531, 810, 1000, 1305, 1613
Eco88I CYCGRG 1 cut(s) 1214
EcoICRI GAGCTC 1 cut(s) 1555
EcoO109I RGGNCCY 1 cut(s) 983
EcoRI GAATTC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 41, 1599
EcoT22I ATGCAT 1 cut(s) 163
EcoT38I GRGCYC 2 cut(s) 1537, 1557
ErhI CCWWGG 2 cut(s) 41, 1599
FaeI CATG 5 cut(s) 104, 158, 521, 641, 697
FaqI GGGAC 2 cut(s) 969, 1447
FatI CATG 5 cut(s) 100, 154, 517, 637, 693
Fnu4HI GCNGC 3 cut(s) 760, 1170, 1265
FokI GGATG 3 cut(s) 140, 1322, 1343
FriOI GRGCYC 2 cut(s) 1537, 1557
Fsp4HI GCNGC 3 cut(s) 760, 1170, 1265
FspBI CTAG 3 cut(s) 62, 1409, 1457
FspI TGCGCA 1 cut(s) 1368
GlaI GCGC 1 cut(s) 1368
GluI GCNGC 3 cut(s) 760, 1170, 1265
GsaI CCCAGC 1 cut(s) 628
GsuI CTGGAG 1 cut(s) 1121
HaeIII GGCC 2 cut(s) 759, 1418
HapII CCGG 1 cut(s) 660
HhaI GCGC 1 cut(s) 1369
Hin1II CATG 5 cut(s) 104, 158, 521, 641, 697
Hin6I GCGC 1 cut(s) 1367
HinP1I GCGC 1 cut(s) 1367
HindIII AAGCTT 2 cut(s) 1012, 1120
HinfI GANTC 7 cut(s) 352, 1082, 1187, 1316, 1387, 1439, 1460
HpaII CCGG 1 cut(s) 660
HphI GGTGA 3 cut(s) 445, 1172, 1270
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 8 cut(s) 379, 778, 1003, 1081, 1485, 1525, 1542, 1593
Hpy8I GTNNAC 1 cut(s) 326
HpyAV CCTTC 5 cut(s) 552, 690, 888, 974, 1008
HpyCH4III ACNGT 4 cut(s) 24, 1109, 1424, 1582
HpyCH4V TGCA 3 cut(s) 161, 293, 1064
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 4 cut(s) 117, 423, 1024, 1541
Hsp92II CATG 5 cut(s) 104, 158, 521, 641, 697
HspAI GCGC 1 cut(s) 1367
LguI GCTCTTC 2 cut(s) 507, 636
LmnI GCTCC 3 cut(s) 302, 737, 1546
Lsp1109I GCAGC 2 cut(s) 1181, 1276
LweI GCATC 5 cut(s) 148, 394, 1051, 1402, 1495
MaeI CTAG 3 cut(s) 62, 1409, 1457
MfeI CAATTG 2 cut(s) 1181, 1191
MflI RGATCY 6 cut(s) 338, 361, 578, 656, 773, 874
MhlI GDGCHC 2 cut(s) 1537, 1557
MlyI GAGTC 3 cut(s) 346, 1396, 1469
MmeI TCCRAC 1 cut(s) 1611
MnlI CCTC 9 cut(s) 112, 227, 366, 916, 958, 1223, 1465, 1489, 1536
Mph1103I ATGCAT 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 1611
MseI TTAA 3 cut(s) 255, 287, 675
MslI CAYNNNNRTG 4 cut(s) 110, 522, 972, 1256
MspA1I CMGCKG 1 cut(s) 1267
MspI CCGG 1 cut(s) 660
MspR9I CCNGG 1 cut(s) 173
MunI CAATTG 2 cut(s) 1181, 1191
Mva1269I GAATGC 1 cut(s) 1400
MvaI CCWGG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 158
NlaIII CATG 5 cut(s) 104, 158, 521, 641, 697
NlaIV GGNNCC 4 cut(s) 298, 658, 733, 985
NsbI TGCGCA 1 cut(s) 1368
NsiI ATGCAT 1 cut(s) 163
NspV TTCGAA 1 cut(s) 1404
OliI CACNNNNGTG 2 cut(s) 972, 1256
PaeR7I CTCGAG 1 cut(s) 1214
PciSI GCTCTTC 2 cut(s) 507, 636
PctI GAATGC 1 cut(s) 1400
PdmI GAANNNNTTC 1 cut(s) 1611
PfeI GAWTC 4 cut(s) 1082, 1187, 1316, 1439
PflFI GACNNNGTC 2 cut(s) 1273, 1637
PkrI GCNGC 3 cut(s) 761, 1171, 1266
PleI GAGTC 3 cut(s) 346, 1395, 1468
PpsI GAGTC 3 cut(s) 346, 1395, 1468
PpuMI RGGWCCY 1 cut(s) 983
Psp124BI GAGCTC 1 cut(s) 1557
Psp5II RGGWCCY 1 cut(s) 983
Psp6I CCWGG 1 cut(s) 171
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 4 cut(s) 298, 658, 733, 985
PspPI GGNCC 2 cut(s) 854, 983
PspPPI RGGWCCY 1 cut(s) 983
PspXI VCTCGAGB 1 cut(s) 1214
PsuI RGATCY 6 cut(s) 338, 361, 578, 656, 773, 874
PsyI GACNNNGTC 2 cut(s) 1273, 1637
PvuII CAGCTG 1 cut(s) 1267
RsaI GTAC 4 cut(s) 10, 26, 810, 1106
RsaNI GTAC 4 cut(s) 9, 25, 809, 1105
RseI CAYNNNNRTG 4 cut(s) 110, 522, 972, 1256
SacI GAGCTC 1 cut(s) 1557
SapI GCTCTTC 2 cut(s) 507, 636
SaqAI TTAA 3 cut(s) 255, 287, 675
SatI GCNGC 3 cut(s) 760, 1170, 1265
Sau96I GGNCC 2 cut(s) 854, 983
ScaI AGTACT 1 cut(s) 26
SchI GAGTC 3 cut(s) 346, 1396, 1469
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 2 cut(s) 1537, 1557
SfaNI GCATC 5 cut(s) 148, 394, 1051, 1402, 1495
SfcI CTRYAG 1 cut(s) 20
Sfr274I CTCGAG 1 cut(s) 1214
SfuI TTCGAA 1 cut(s) 1404
SinI GGWCC 2 cut(s) 854, 983
SlaI CTCGAG 1 cut(s) 1214
SmiMI CAYNNNNRTG 4 cut(s) 110, 522, 972, 1256
SmlI CTYRAG 5 cut(s) 357, 864, 1214, 1304, 1550
SmoI CTYRAG 5 cut(s) 357, 864, 1214, 1304, 1550
SsiI CCGC 2 cut(s) 760, 857
SspI AATATT 1 cut(s) 136
SspMI CTAG 3 cut(s) 62, 1409, 1457
SstI GAGCTC 1 cut(s) 1557
StyD4I CCNGG 1 cut(s) 171
StyI CCWWGG 2 cut(s) 41, 1599
TaaI ACNGT 4 cut(s) 24, 1109, 1424, 1582
TaqI TCGA 4 cut(s) 1142, 1215, 1396, 1404
TaqII GACCGA 1 cut(s) 396
TatI WGTACW 1 cut(s) 24
TauI GCSGC 1 cut(s) 762
TfiI GAWTC 4 cut(s) 1082, 1187, 1316, 1439
Tru1I TTAA 3 cut(s) 255, 287, 675
Tru9I TTAA 3 cut(s) 255, 287, 675
TscAI CASTG 5 cut(s) 73, 232, 921, 994, 1093
TseI GCWGC 2 cut(s) 1169, 1264
TspDTI ATGAA 9 cut(s) 69, 89, 135, 201, 534, 534, 654, 723, 1374
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 5 cut(s) 73, 232, 921, 994, 1093
Tth111I GACNNNGTC 2 cut(s) 1273, 1637
VpaK11BI GGWCC 2 cut(s) 854, 983
XapI RAATTY 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 1 cut(s) 1093
XhoI CTCGAG 1 cut(s) 1214
XmnI GAANNNNTTC 1 cut(s) 1611
XspI CTAG 3 cut(s) 62, 1409, 1457
ZrmI AGTACT 1 cut(s) 26
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.