Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0005899

Physical Location & Seq

Genomic Coordinates Forward (+)

13960489 .. 13963830

Gene Structure 3342 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g15960.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGTTCAAAGTCAAACAGTTGAACCCCAAATGCTATCGTGCCGCTGCAAGACAGTGAAGAACAAGAGGACTATTATCGCTGGCCTAAATTCAGATACCTGCAGTAGAGAAATGCTGCTTGGATTGCTCACTTCTGTTGTTAAACCAGAAGACAATGTGATAGCTATTCATGTGGAAGAAACAGATGATACATTTGATCCAAACACTTTCCACATTCATGAAGATATCTGCAAGTCCAAGAAGGTAGATTTTCAAGTAAAGGTCTGCATAGGAGATTCATACATTTCTGAATTAACTTACCAAGTTAGAGTCAGCTATGCTACAATCCTCACACTTGGTTGCAGCTCTTCAGGGCTCAATGTTTCAGCTATCAGTGCTTGCCTGAAAGGATTACCTCCTTCGTGCACACTTCTGGTTATGAATAGTGTTGGAAGAATTTTGTTTCAGAGGGAGGGAACTTGCCAACAAGGTTCTGTAAAGATAGCCCGGCCCTTGCAATCTTCGCAGTCATTTTCTTCAGGGTACACCTGTTATAATCAGTCAAATACATCCCGGAAATTGCATAAGTCATTGACAATACCATCTGCTCCTCCTGTATCATCAATGCGGCGATTTACTAGAAGGGCACTCTCTTCTGCACATAAAACAGTGGAGGTTTCTGATTTTGTGGCTCAAAAACTGTTTCATAGATTGGCACTACTGGAAGCAGAAGGTTCCAGCAGGCATTTCGCCTCCCAAGAGCTTCGGTATGCAACTAACAATTTCAGCCCTGACATGGTGATTGGAGAAGGTGGACATAGCAAGGTGTATCGAGCCACTCTTGAAGATGGTAGAGGTGCAGCTGTGAAAGTCCTCAAAACCACACACCATTCAGTCAATGATCTTTTCCGCGAAGTAGATTTTCTGTCTAGTATGAATCACAAGAACATAATTCAGATAATTGGGTTCTGTGATAGTGGAGAGATGCTTGCGATTGTGTATGATCTTTTACATGGAAGCTTGAGGAGGAATTTGAGACAACTAAGGTGGAGTGAGAGGATGAAAGTTGCACTTGGTGTAGCAAAAGCTTTAGAGTACCTTCATCATTCTCACAACCCTCCCATCATTCATAGGGATGTCAAGTCTTCTAACATTCTCCTCTCCCATGATTGCGAACCAATATTGTCCGATTTTGGAGCAGCAATGGTACTTCGTCAATCTCAGCATGCAGACGTGCCATGTGATGTTGTTGGGACGTTTGGATACTTAGCCCCAGAGTACATGATGTATGGCAAGGTTGATGAGAAGATAGATGTGTACTCATATGGGGTGGTGCTTTTAGAACTCATTACTGGGAAAGAGGCTATTCAAACCGACCAGGAGATTCGAGAGAGCTTAGTACTTTGGTCTTTGCTTATTGATGTGCAGGCAAGGTCCCTACTGAGCTGTGGCATATGCGAACGTCTAATTGATCCTTACTTGTCTGAGGAGTATAACAAGGAAGAGATGGAAGTAATGATGATAGCTGCACGCCTCTGCCTTATGCATTCATCTTCTAGAAGACCAACCATGAAAACGATACTTAGGCTATTTGAGGAGCCAGATCATTGGTTAAGGATGCAGAGAGAAAGAGATGAGTTTCTGAAAGGGACTAATTCAAAAGATGAAACAGTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

553

Amino Acids

62.07

Weight (kDa)

7.04

Isoelectric Point (pI)

50.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	259 - 520	1.3e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	260 - 522	7.4e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005899)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g15960 FvH4_2g15960 FvH4_2g15960
malus_domestica	MD05G1128600.v1.1
prunus_persica	Prupe.8G172700_v2.0.a1 Prupe.8G172700_v2.0.a1 Prupe.8G172700_v2.0.a1
pyrus_communis	pycom05g12450
rosa_chinensis	RchiOBHm_Chr6g0279501
rosa_laevigata	RLG00000013142 RLG00000013420
rosa_multiflora	Rmu_sc0004649.1_g000022 Rmu_sc0009253.1_g000001 Rmu_sc0027163.1_g000002
rosa_roxburghii	Rroxscaffold_7G00188870 Rroxscaffold_7G00192750
rosa_rugosa	Rorug06G0097700 Rorug06G0124800 Rorug06G0124900 Rorug06G0125000
rosa_samantha	Rh6AG209200 Rh6AG234000 Rh6BG213400 Rh6BG238200 Rh6CG216200 Rh6CG240300 Rh6DG231900
rosa_wichuraiana	Rw6G020410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	534
Acc36I	ACCTGC	1 cut(s)	107
AccII	CGCG	1 cut(s)	891
AciI	CCGC	3 cut(s)	43, 607, 889
AclWI	GGATC	2 cut(s)	191, 1445
AcsI	RAATTY	3 cut(s)	88, 435, 1009
AcuI	CTGAAG	2 cut(s)	333, 501
AdeI	CACNNNGTG	1 cut(s)	1055
AfaI	GTAC	6 cut(s)	524, 1076, 1188, 1259, 1298, 1380
AfiI	CCNNNNNNNGG	1 cut(s)	775
AgsI	TTSAA	6 cut(s)	8, 23, 254, 824, 1349, 1638
AjiI	CACGTC	1 cut(s)	1213
AjnI	CCWGG	1 cut(s)	1356
AjuI	GAANNNNNNNTTGG	2 cut(s)	102, 134
Alw21I	GWGCWC	1 cut(s)	407
Alw26I	GTCTC	1 cut(s)	1009
Alw44I	GTGCAC	1 cut(s)	403
AlwI	GGATC	2 cut(s)	191, 1445
AoxI	GGCC	2 cut(s)	82, 488
ApaLI	GTGCAC	1 cut(s)	403
ApeKI	GCWGC	6 cut(s)	45, 115, 342, 839, 1178, 1505
ApoI	RAATTY	3 cut(s)	88, 435, 1009
AspS9I	GGNCC	2 cut(s)	489, 1413
AsuC2I	CCSGG	2 cut(s)	487, 553
AsuHPI	GGTGA	1 cut(s)	790
AvaII	GGWCC	1 cut(s)	1413
BaeGI	GKGCMC	2 cut(s)	407, 628
BaeI	ACNNNNGTAYC	2 cut(s)	514, 547
BanII	GRGCYC	1 cut(s)	357
BbsI	GAAGAC	3 cut(s)	156, 1116, 1546
Bbv12I	GWGCWC	1 cut(s)	407
BbvI	GCAGC	6 cut(s)	32, 102, 354, 851, 1190, 1492
BccI	CCATC	4 cut(s)	589, 821, 1109, 1480
BcgI	CGANNNNNNTGC	2 cut(s)	709, 743
BciT130I	CCWGG	1 cut(s)	1358
BciVI	GTATCC	1 cut(s)	1235
BcnI	CCSGG	2 cut(s)	487, 553
BcoDI	GTCTC	1 cut(s)	1009
BfaI	CTAG	3 cut(s)	618, 909, 1536
BfmI	CTRYAG	1 cut(s)	100
BfuAI	ACCTGC	1 cut(s)	107
BfuI	GTATCC	1 cut(s)	1235
BisI	GCNGC	8 cut(s)	43, 46, 116, 343, 608, 840, 1179, 1506
BlsI	GCNGC	8 cut(s)	44, 47, 117, 344, 609, 841, 1180, 1507
BmcAI	AGTACT	1 cut(s)	1380
Bme1390I	CCNGG	3 cut(s)	487, 553, 1358
Bme18I	GGWCC	1 cut(s)	1413
BmgBI	CACGTC	1 cut(s)	1213
BmgT120I	GGNCC	2 cut(s)	489, 1413
BmiI	GGNNCC	3 cut(s)	715, 1415, 1578
BmrFI	CCNGG	3 cut(s)	487, 553, 1358
BmrI	ACTGGG	1 cut(s)	1341
BmsI	GCATC	2 cut(s)	954, 1587
BmuI	ACTGGG	1 cut(s)	1341
BpiI	GAAGAC	3 cut(s)	156, 1116, 1546
BpuEI	CTTGAG	1 cut(s)	1021
BpuMI	CCSGG	2 cut(s)	487, 553
Bsc4I	CCNNNNNNNGG	1 cut(s)	775
Bse1I	ACTGG	2 cut(s)	705, 1336
Bse3DI	GCAATG	1 cut(s)	1188
BseBI	CCWGG	1 cut(s)	1358
BseGI	GGATG	4 cut(s)	548, 1044, 1120, 1602
BseLI	CCNNNNNNNGG	1 cut(s)	775
BseMI	GCAATG	1 cut(s)	1188
BseMII	CTCAG	3 cut(s)	1214, 1412, 1455
BseNI	ACTGG	2 cut(s)	705, 1336
BseRI	GAGGAG	5 cut(s)	579, 1018, 1127, 1481, 1589
BseSI	GKGCMC	2 cut(s)	407, 628
BseXI	GCAGC	6 cut(s)	32, 102, 354, 851, 1190, 1492
BsgI	GTGCAG	4 cut(s)	621, 858, 1424, 1491
Bsh1236I	CGCG	1 cut(s)	891
BshFI	GGCC	2 cut(s)	84, 490
BsiHKAI	GWGCWC	1 cut(s)	407
BsiSI	CCGG	2 cut(s)	487, 553
BslFI	GGGAC	3 cut(s)	1246, 1399, 1642
BslI	CCNNNNNNNGG	1 cut(s)	775
BsmAI	GTCTC	1 cut(s)	1009
BsmFI	GGGAC	3 cut(s)	1246, 1399, 1642
BsmI	GAATGC	1 cut(s)	1525
BsnI	GGCC	2 cut(s)	84, 490
Bsp1286I	GDGCHC	3 cut(s)	357, 407, 628
Bsp143I	GATC	5 cut(s)	196, 880, 982, 1450, 1582
BspACI	CCGC	3 cut(s)	43, 607, 889
BspANI	GGCC	2 cut(s)	84, 490
BspCNI	CTCAG	3 cut(s)	1213, 1413, 1456
BspFNI	CGCG	1 cut(s)	891
BspHI	TCATGA	1 cut(s)	217
BspLI	GGNNCC	3 cut(s)	715, 1415, 1578
BspMAI	CTGCAG	1 cut(s)	104
BspMI	ACCTGC	1 cut(s)	107
BspPI	GGATC	2 cut(s)	191, 1445
BspQI	GCTCTTC	1 cut(s)	352
BsrDI	GCAATG	1 cut(s)	1188
BsrI	ACTGG	2 cut(s)	705, 1336
BssMI	GATC	5 cut(s)	196, 880, 982, 1450, 1582
Bst2UI	CCWGG	1 cut(s)	1358
Bst4CI	ACNGT	5 cut(s)	19, 55, 649, 681, 1651
Bst6I	CTCTTC	3 cut(s)	352, 637, 1476
BstC8I	GCNNGC	7 cut(s)	82, 379, 722, 969, 1206, 1407, 1510
BstDEI	CTNAG	7 cut(s)	1022, 1200, 1246, 1375, 1421, 1464, 1562
BstF5I	GGATG	4 cut(s)	548, 1044, 1120, 1602
BstFNI	CGCG	1 cut(s)	891
BstKTI	GATC	5 cut(s)	199, 883, 985, 1453, 1585
BstMAI	GTCTC	1 cut(s)	1009
BstMBI	GATC	5 cut(s)	196, 880, 982, 1450, 1582
BstMWI	GCNNNNNNNGC	3 cut(s)	124, 374, 502
BstNI	CCWGG	1 cut(s)	1358
BstNSI	RCATGY	1 cut(s)	1208
BstSCI	CCNGG	3 cut(s)	485, 551, 1356
BstSFI	CTRYAG	1 cut(s)	100
BstSLI	GKGCMC	2 cut(s)	407, 628
BstUI	CGCG	1 cut(s)	891
BstV1I	GCAGC	6 cut(s)	32, 102, 354, 851, 1190, 1492
BstV2I	GAAGAC	3 cut(s)	156, 1116, 1546
BstXI	CCANNNNNNTGG	1 cut(s)	1587
BsuI	GTATCC	1 cut(s)	1235
BsuRI	GGCC	2 cut(s)	84, 490
BtrI	CACGTC	1 cut(s)	1213
BtsCI	GGATG	4 cut(s)	548, 1044, 1120, 1602
BtsIMutI	CAGTG	3 cut(s)	60, 379, 654
BveI	ACCTGC	1 cut(s)	107
Cac8I	GCNNGC	7 cut(s)	82, 379, 722, 969, 1206, 1407, 1510
CciI	TCATGA	1 cut(s)	217
Cfr13I	GGNCC	2 cut(s)	489, 1413
Csp6I	GTAC	6 cut(s)	523, 1075, 1187, 1258, 1297, 1379
CspCI	CAANNNNNGTGG	2 cut(s)	1007, 1042
CviAII	CATG	9 cut(s)	170, 218, 775, 992, 1145, 1205, 1218, 1261, 1549
CviQI	GTAC	6 cut(s)	523, 1075, 1187, 1258, 1297, 1379
DdeI	CTNAG	7 cut(s)	1022, 1200, 1246, 1375, 1421, 1464, 1562
DpnI	GATC	5 cut(s)	198, 882, 984, 1452, 1584
DpnII	GATC	5 cut(s)	196, 880, 982, 1450, 1582
DraIII	CACNNNGTG	1 cut(s)	1055
Eam1104I	CTCTTC	3 cut(s)	352, 637, 1476
EarI	CTCTTC	3 cut(s)	352, 637, 1476
Eco24I	GRGCYC	1 cut(s)	357
Eco32I	GATATC	1 cut(s)	226
Eco47I	GGWCC	1 cut(s)	1413
Eco57I	CTGAAG	2 cut(s)	333, 501
EcoO109I	RGGNCCY	1 cut(s)	1413
EcoRII	CCWGG	1 cut(s)	1356
EcoRV	GATATC	1 cut(s)	226
EcoT22I	ATGCAT	1 cut(s)	1527
EcoT38I	GRGCYC	1 cut(s)	357
FaeI	CATG	9 cut(s)	173, 221, 778, 995, 1148, 1208, 1221, 1264, 1552
FalI	AAGNNNNNCTT	2 cut(s)	1035, 1067
FaqI	GGGAC	3 cut(s)	1246, 1399, 1642
FatI	CATG	9 cut(s)	169, 217, 774, 991, 1144, 1204, 1217, 1260, 1548
FauNDI	CATATG	2 cut(s)	1303, 1433
Fnu4HI	GCNGC	8 cut(s)	43, 46, 116, 343, 608, 840, 1179, 1506
FokI	GGATG	4 cut(s)	535, 1051, 1127, 1609
FriOI	GRGCYC	1 cut(s)	357
Fsp4HI	GCNGC	8 cut(s)	43, 46, 116, 343, 608, 840, 1179, 1506
FspBI	CTAG	3 cut(s)	618, 909, 1536
GluI	GCNGC	8 cut(s)	43, 46, 116, 343, 608, 840, 1179, 1506
HaeIII	GGCC	2 cut(s)	84, 490
HapII	CCGG	2 cut(s)	487, 553
Hin1II	CATG	9 cut(s)	173, 221, 778, 995, 1148, 1208, 1221, 1264, 1552
HindIII	AAGCTT	2 cut(s)	997, 1065
HinfI	GANTC	4 cut(s)	275, 309, 916, 1363
HpaII	CCGG	2 cut(s)	487, 553
HphI	GGTGA	1 cut(s)	790
Hpy166II	GTNNAC	4 cut(s)	405, 525, 794, 1297
Hpy188I	TCNGA	8 cut(s)	94, 289, 447, 661, 936, 1168, 1465, 1623
Hpy188III	TCNNGA	4 cut(s)	218, 821, 1367, 1536
Hpy8I	GTNNAC	4 cut(s)	405, 525, 794, 1297
HpyAV	CCTTC	6 cut(s)	235, 408, 615, 704, 782, 1088
HpyCH4III	ACNGT	5 cut(s)	19, 55, 649, 681, 1651
HpyCH4IV	ACGT	3 cut(s)	1212, 1235, 1441
HpyF10VI	GCNNNNNNNGC	3 cut(s)	124, 374, 502
HpyF3I	CTNAG	7 cut(s)	1022, 1200, 1246, 1375, 1421, 1464, 1562
HpySE526I	ACGT	3 cut(s)	1212, 1235, 1441
Hsp92II	CATG	9 cut(s)	173, 221, 778, 995, 1148, 1208, 1221, 1264, 1552
Kzo9I	GATC	5 cut(s)	196, 880, 982, 1450, 1582
LguI	GCTCTTC	1 cut(s)	352
LmnI	GCTCC	3 cut(s)	592, 1175, 1576
Lsp1109I	GCAGC	6 cut(s)	32, 102, 354, 851, 1190, 1492
LweI	GCATC	2 cut(s)	954, 1587
MaeI	CTAG	3 cut(s)	618, 909, 1536
MaeII	ACGT	3 cut(s)	1212, 1235, 1441
MaeIII	GTNAC	1 cut(s)	1651
MalI	GATC	5 cut(s)	198, 882, 984, 1452, 1584
MboI	GATC	5 cut(s)	196, 880, 982, 1450, 1582
MhlI	GDGCHC	3 cut(s)	357, 407, 628
MlyI	GAGTC	1 cut(s)	318
MmeI	TCCRAC	1 cut(s)	409
Mph1103I	ATGCAT	1 cut(s)	1527
MseI	TTAA	3 cut(s)	141, 293, 1592
MslI	CAYNNNNRTG	2 cut(s)	216, 1113
MspA1I	CMGCKG	2 cut(s)	45, 842
MspI	CCGG	2 cut(s)	487, 553
MspR9I	CCNGG	3 cut(s)	487, 553, 1358
Mva1269I	GAATGC	1 cut(s)	1525
MvaI	CCWGG	1 cut(s)	1358
MvnI	CGCG	1 cut(s)	891
MwoI	GCNNNNNNNGC	3 cut(s)	124, 374, 502
NciI	CCSGG	2 cut(s)	487, 553
NdeI	CATATG	2 cut(s)	1303, 1433
NdeII	GATC	5 cut(s)	196, 880, 982, 1450, 1582
NlaIII	CATG	9 cut(s)	173, 221, 778, 995, 1148, 1208, 1221, 1264, 1552
NlaIV	GGNNCC	3 cut(s)	715, 1415, 1578
NsiI	ATGCAT	1 cut(s)	1527
NspI	RCATGY	1 cut(s)	1208
PaeI	GCATGC	1 cut(s)	1208
PagI	TCATGA	1 cut(s)	217
PciSI	GCTCTTC	1 cut(s)	352
PctI	GAATGC	1 cut(s)	1525
PfeI	GAWTC	3 cut(s)	275, 916, 1363
PfoI	TCCNGGA	1 cut(s)	551
PkrI	GCNGC	8 cut(s)	44, 47, 117, 344, 609, 841, 1180, 1507
PleI	GAGTC	1 cut(s)	317
PpsI	GAGTC	1 cut(s)	317
PpuMI	RGGWCCY	1 cut(s)	1413
PsiI	TTATAA	1 cut(s)	534
Psp5II	RGGWCCY	1 cut(s)	1413
Psp6I	CCWGG	1 cut(s)	1356
PspGI	CCWGG	1 cut(s)	1356
PspN4I	GGNNCC	3 cut(s)	715, 1415, 1578
PspPI	GGNCC	2 cut(s)	489, 1413
PspPPI	RGGWCCY	1 cut(s)	1413
PstI	CTGCAG	1 cut(s)	104
PvuII	CAGCTG	1 cut(s)	842
RsaI	GTAC	6 cut(s)	524, 1076, 1188, 1259, 1298, 1380
RsaNI	GTAC	6 cut(s)	523, 1075, 1187, 1258, 1297, 1379
RseI	CAYNNNNRTG	2 cut(s)	216, 1113
SapI	GCTCTTC	1 cut(s)	352
SaqAI	TTAA	3 cut(s)	141, 293, 1592
SatI	GCNGC	8 cut(s)	43, 46, 116, 343, 608, 840, 1179, 1506
Sau3AI	GATC	5 cut(s)	196, 880, 982, 1450, 1582
Sau96I	GGNCC	2 cut(s)	489, 1413
ScaI	AGTACT	1 cut(s)	1380
SchI	GAGTC	1 cut(s)	318
ScrFI	CCNGG	3 cut(s)	487, 553, 1358
SduI	GDGCHC	3 cut(s)	357, 407, 628
SfaNI	GCATC	2 cut(s)	954, 1587
SfcI	CTRYAG	1 cut(s)	100
SinI	GGWCC	1 cut(s)	1413
SmiMI	CAYNNNNRTG	2 cut(s)	216, 1113
SmlI	CTYRAG	1 cut(s)	1000
SmoI	CTYRAG	1 cut(s)	1000
SphI	GCATGC	1 cut(s)	1208
SsiI	CCGC	3 cut(s)	43, 607, 889
SspI	AATATT	1 cut(s)	1161
SspMI	CTAG	3 cut(s)	618, 909, 1536
StyD4I	CCNGG	3 cut(s)	485, 551, 1356
TaaI	ACNGT	5 cut(s)	19, 55, 649, 681, 1651
TaiI	ACGT	3 cut(s)	1215, 1238, 1444
TaqI	TCGA	2 cut(s)	811, 1366
TatI	WGTACW	3 cut(s)	1257, 1296, 1378
TauI	GCSGC	2 cut(s)	45, 610
TfiI	GAWTC	3 cut(s)	275, 916, 1363
Tru1I	TTAA	3 cut(s)	141, 293, 1592
Tru9I	TTAA	3 cut(s)	141, 293, 1592
TscAI	CASTG	3 cut(s)	60, 379, 654
TseI	GCWGC	6 cut(s)	45, 115, 342, 839, 1178, 1505
TspRI	CASTG	3 cut(s)	60, 379, 654
VneI	GTGCAC	1 cut(s)	403
VpaK11BI	GGWCC	1 cut(s)	1413
XapI	RAATTY	3 cut(s)	88, 435, 1009
XbaI	TCTAGA	1 cut(s)	1535
XceI	RCATGY	1 cut(s)	1208
XspI	CTAG	3 cut(s)	618, 909, 1536
ZrmI	AGTACT	1 cut(s)	1380
Zsp2I	ATGCAT	1 cut(s)	1527

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.