Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004417

Physical Location & Seq

Genomic Coordinates Reverse (-)

14726684 .. 14730172

Gene Structure 3489 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g16990.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1332 bp

ATGGAGCTGCTCCTCCTCCGTCTCTGCTTACTCTCCCTTCTCCTCCTCTCATCAAAACCCATCTCCGCAGAAGAACAACAAGACTCAGTAATCTCAATGTTCCAAAAATACCTCCAAATCAACACCGCCCACCCAAAACCCCTGTACCAAGAAGCAGCCGATTTCATCCTCACCGAGGCCAACTCCCTCTCCCTTCAATCCCAGACCCTCGAGTTCGTCCCCGGCAAGCCCCTCGTCCTCCTCAAATGGCCCGGCTCCGACCCTTCTCTCCCTTCCATCCTCCTCAACTCCCACACCGACGTCGTCCCCGCCGAGCACGACAAGTGGGCCCACCACCCCTTCGCCGCCCACGTCGACCCGAATGGTAAAATCTACGCCAGAGGCTCCCAGGACATGAAGTGCGTCGGGCTTCAGTACCTGGAGGCCATTCGTCGTCTGAAGACTTCTGGGTTTGTGCCCACTCGGAGCGTTTACTTGTCTTTTGTTCCGGATGAGGAGATTGGCGGCCATGACGGCGCTGAGAAGTTTGCTGAGTCTGAGATTTTCAAATCCTTGAACGTTGGGATAGTACTTGATGAAGGGTTGGCGTCACCGAGTGAGAACTACAGGGCGTTTTATGCGGAGAGGTGCCCGTGGTGGCTGATCATCAAGGCTACCGGGGCACCGGGGCATGGGGCGAAGCTGTATGACAATACTGCCATGGAGAATATATTGAAGAGCATTGAGAGTGTGAGGAGGTTCCGGGCAGCGCAGTTTGATTTGGTGAAGGCGGGATTGAAGGCTGAAGGGGAGGTTGTCTCGGTTAATATGGCATTTTTGAAGGCTGGCACACCGAGTCCGACTGGTTTTGTCATGAATTTGCAGCCATCTGAAGCAGAGGCAGGGTTTGATATTAGGGTCCCCCCAACTGCTGATCAGGAGTCTCTGGAAAAACGAATTGCTGAAGAATGGGCGCCCACTTCACGCAACATGACTTTTAGGTTCAAGCAGAAGGTTTCGGTGCTTGATAAGTCTGGGAGGCCAGTCCTTACAGCAACTGACAGTTCAAATCCCTGGTGGGCCCTGCTAGAAGAAGCAGTCAAAAAAGCTAACGGAAAACTTGGAAAGCCTGAGATTTTTCCTGCATCAACAGATGCTCGCTATTTCCGGTTACAAGGCCTGCCAGCAATTGGATTCTCCCCTATGGCTAACACTCCTATTCTGCTCCATGACCATAACGAGTTTCTAAACAAAGACGAATACTTGAAAGGAATCAAAATTTATGAATTCATTATCAAATCTTATTCATCTTATGCTGAGCATGTGACAACGGGCATGAGAGATGAGTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004046 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005615 GO:0016787 GO:0016810 GO:0016811 GO:0031982 GO:0042802 GO:0043226 GO:0043227 GO:0043230 GO:0044421 GO:0070062 GO:1903561

KEGG Pathways

Metabolic & Signaling

ko00220 ko01100 ko01110 ko01130 ko01210 ko01230 map00220 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

444

Amino Acids

49.3

Weight (kDa)

5.94

Isoelectric Point (pI)

41.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	94 - 426	2.5e-37	Peptidase family M20/M25/M40

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004417)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38220 AT4G38220
fragaria_vesca	FvH4_2g16990
malus_domestica	MD05G1143000.v1.1 MD10G1142800.v1.1 MD15G1413100.v1.1
prunus_persica	Prupe.8G181800_v2.0.a1 Prupe.8G181800_v2.0.a1
pyrus_communis	pycom05g13750 pycom10g12270
rosa_chinensis	RchiOBHm_Chr2g0126171 RchiOBHm_Chr6g0281181
rosa_laevigata	RLG00000012987
rosa_multiflora	Rmu_co8196198.1_g000001 Rmu_co8363365.1_g000001 Rmu_sc0001705.1_g000022 Rmu_sc0012209.1_g000001 Rmu_sc0026953.1_g000001 Rmu_sc0034918.1_g000001 Rmu_sc0035650.1_g000001
rosa_rugosa	Rorug06G0139500 Rorug06G0139500
rosa_samantha	Rh2BG327100 Rh6AG251300 Rh6BG182200 Rh6BG254500 Rh6CG253900 Rh6DG246000
rosa_wichuraiana	Rw2G031780 Rw3G017710 Rw6G021830 Rw7G031480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	303
AccB1I	GGYRCC	3 cut(s)	627, 661, 952
AccB7I	CCANNNNNTGG	1 cut(s)	1169
AccI	GTMKAC	1 cut(s)	354
AccIII	TCCGGA	1 cut(s)	487
AciI	CCGC	7 cut(s)	66, 126, 309, 345, 504, 620, 770
AclI	AACGTT	1 cut(s)	558
AcoI	YGGCCR	1 cut(s)	505
AcsI	RAATTY	3 cut(s)	856, 1257, 1265
AcuI	CTGAAG	5 cut(s)	395, 458, 804, 891, 963
AcyI	GRCGYC	3 cut(s)	300, 587, 953
AdeI	CACNNNGTG	1 cut(s)	596
AfaI	GTAC	3 cut(s)	146, 416, 570
AfiI	CCNNNNNNNGG	3 cut(s)	175, 671, 1169
AgsI	TTSAA	9 cut(s)	197, 547, 556, 715, 778, 820, 985, 1047, 1246
AjiI	CACGTC	1 cut(s)	352
AjnI	CCWGG	3 cut(s)	387, 417, 1052
AluBI	AGCT	3 cut(s)	7, 682, 1088
AluI	AGCT	3 cut(s)	7, 682, 1088
Alw21I	GWGCWC	1 cut(s)	318
Alw26I	GTCTC	3 cut(s)	26, 802, 927
AlwNI	CAGNNNCTG	2 cut(s)	418, 1037
Ama87I	CYCGRG	1 cut(s)	209
Aor13HI	TCCGGA	1 cut(s)	487
AoxI	GGCC	8 cut(s)	177, 248, 327, 423, 505, 1019, 1059, 1156
ApaI	GGGCCC	2 cut(s)	331, 1063
ApeKI	GCWGC	4 cut(s)	7, 155, 746, 862
ApoI	RAATTY	3 cut(s)	856, 1257, 1265
AspLEI	GCGC	3 cut(s)	518, 751, 955
AspS9I	GGNCC	6 cut(s)	249, 327, 328, 898, 1059, 1060
AsuC2I	CCSGG	5 cut(s)	222, 252, 658, 666, 743
AsuHPI	GGTGA	3 cut(s)	163, 582, 775
AvaI	CYCGRG	1 cut(s)	209
AvaII	GGWCC	1 cut(s)	898
BaeGI	GKGCMC	5 cut(s)	331, 459, 632, 664, 1063
BaeI	ACNNNNGTAYC	2 cut(s)	128, 161
BanI	GGYRCC	3 cut(s)	627, 661, 952
BanII	GRGCYC	2 cut(s)	331, 1063
BbsI	GAAGAC	1 cut(s)	446
Bbv12I	GWGCWC	1 cut(s)	318
BbvI	GCAGC	3 cut(s)	167, 758, 874
BccI	CCATC	3 cut(s)	68, 284, 874
BceAI	ACGGC	1 cut(s)	529
BcgI	CGANNNNNNTGC	2 cut(s)	214, 248
BciT130I	CCWGG	3 cut(s)	389, 419, 1054
BclI	TGATCA	2 cut(s)	642, 913
BcnI	CCSGG	5 cut(s)	222, 252, 658, 666, 743
BcoDI	GTCTC	3 cut(s)	26, 802, 927
BfaI	CTAG	1 cut(s)	1067
BfmI	CTRYAG	1 cut(s)	604
BfoI	RGCGCY	2 cut(s)	519, 956
BglI	GCCNNNNNGGC	1 cut(s)	513
BisI	GCNGC	6 cut(s)	8, 156, 345, 505, 747, 863
BlpI	GCTNAGC	1 cut(s)	1296
BlsI	GCNGC	6 cut(s)	9, 157, 346, 506, 748, 864
BmcAI	AGTACT	1 cut(s)	570
Bme1390I	CCNGG	8 cut(s)	222, 252, 389, 419, 658, 666, 743, 1054
Bme18I	GGWCC	1 cut(s)	898
BmeT110I	CYCGRG	1 cut(s)	209
BmgBI	CACGTC	1 cut(s)	352
BmgT120I	GGNCC	6 cut(s)	249, 327, 328, 898, 1059, 1060
BmrFI	CCNGG	8 cut(s)	222, 252, 389, 419, 658, 666, 743, 1054
BmsI	GCATC	2 cut(s)	1123, 1133
BpiI	GAAGAC	1 cut(s)	446
BplI	GAGNNNNNCTC	4 cut(s)	167, 199, 782, 814
BpmI	CTGGAG	1 cut(s)	440
Bpu1102I	GCTNAGC	1 cut(s)	1296
BpuMI	CCSGG	5 cut(s)	222, 252, 658, 666, 743
BsaHI	GRCGYC	3 cut(s)	300, 587, 953
BsaJI	CCNNGG	8 cut(s)	174, 220, 387, 632, 657, 665, 699, 1052
BsaWI	WCCGGW	2 cut(s)	487, 1146
BsaXI	ACNNNNNCTCC	2 cut(s)	173, 203
Bsc4I	CCNNNNNNNGG	3 cut(s)	175, 671, 1169
Bse1I	ACTGG	2 cut(s)	847, 1022
BseAI	TCCGGA	1 cut(s)	487
BseBI	CCWGG	3 cut(s)	389, 419, 1054
BseDI	CCNNGG	8 cut(s)	174, 220, 387, 632, 657, 665, 699, 1052
BseGI	GGATG	3 cut(s)	165, 276, 496
BseLI	CCNNNNNNNGG	3 cut(s)	175, 671, 1169
BseMII	CTCAG	6 cut(s)	99, 510, 522, 528, 1101, 1287
BseNI	ACTGG	2 cut(s)	847, 1022
BseRI	GAGGAG	7 cut(s)	5, 32, 35, 230, 272, 509, 748
BseSI	GKGCMC	5 cut(s)	331, 459, 632, 664, 1063
BseXI	GCAGC	3 cut(s)	167, 758, 874
BshFI	GGCC	8 cut(s)	179, 250, 329, 425, 507, 1021, 1061, 1158
BshNI	GGYRCC	3 cut(s)	627, 661, 952
BsiHKAI	GWGCWC	1 cut(s)	318
BsiHKCI	CYCGRG	1 cut(s)	209
BsiSI	CCGG	7 cut(s)	222, 252, 488, 657, 665, 742, 1147
BslFI	GGGAC	3 cut(s)	203, 290, 884
BslI	CCNNNNNNNGG	3 cut(s)	175, 671, 1169
BsmAI	GTCTC	3 cut(s)	26, 802, 927
BsmBI	CGTCTC	1 cut(s)	26
BsmFI	GGGAC	3 cut(s)	203, 290, 884
BsnI	GGCC	8 cut(s)	179, 250, 329, 425, 507, 1021, 1061, 1158
BsoBI	CYCGRG	1 cut(s)	209
Bsp120I	GGGCCC	2 cut(s)	327, 1059
Bsp1286I	GDGCHC	6 cut(s)	318, 331, 459, 632, 664, 1063
Bsp13I	TCCGGA	1 cut(s)	487
Bsp143I	GATC	2 cut(s)	642, 913
Bsp1720I	GCTNAGC	1 cut(s)	1296
Bsp19I	CCATGG	1 cut(s)	699
BspACI	CCGC	7 cut(s)	66, 126, 309, 345, 504, 620, 770
BspANI	GGCC	8 cut(s)	179, 250, 329, 425, 507, 1021, 1061, 1158
BspCNI	CTCAG	6 cut(s)	98, 511, 523, 529, 1102, 1288
BspEI	TCCGGA	1 cut(s)	487
BspHI	TCATGA	1 cut(s)	852
BspQI	GCTCTTC	1 cut(s)	710
BspT107I	GGYRCC	3 cut(s)	627, 661, 952
BsrI	ACTGG	2 cut(s)	847, 1022
BssECI	CCNNGG	8 cut(s)	174, 220, 387, 632, 657, 665, 699, 1052
BssMI	GATC	2 cut(s)	642, 913
BssNI	GRCGYC	3 cut(s)	300, 587, 953
BssT1I	CCWWGG	1 cut(s)	699
Bst2UI	CCWGG	3 cut(s)	389, 419, 1054
Bst4CI	ACNGT	1 cut(s)	1043
Bst6I	CTCTTC	1 cut(s)	710
BstACI	GRCGYC	3 cut(s)	300, 587, 953
BstC8I	GCNNGC	5 cut(s)	227, 826, 1138, 1160, 1164
BstDEI	CTNAG	6 cut(s)	85, 519, 531, 537, 1110, 1296
BstDSI	CCRYGG	2 cut(s)	632, 699
BstENI	CCTNNNNNAGG	1 cut(s)	173
BstF5I	GGATG	3 cut(s)	165, 276, 496
BstH2I	RGCGCY	2 cut(s)	519, 956
BstHHI	GCGC	3 cut(s)	518, 751, 955
BstKTI	GATC	2 cut(s)	645, 916
BstMAI	GTCTC	3 cut(s)	26, 802, 927
BstMBI	GATC	2 cut(s)	642, 913
BstMWI	GCNNNNNNNGC	3 cut(s)	513, 617, 659
BstNI	CCWGG	3 cut(s)	389, 419, 1054
BstNSI	RCATGY	1 cut(s)	1304
BstSCI	CCNGG	8 cut(s)	220, 250, 387, 417, 656, 664, 741, 1052
BstSFI	CTRYAG	1 cut(s)	604
BstSLI	GKGCMC	5 cut(s)	331, 459, 632, 664, 1063
BstV1I	GCAGC	3 cut(s)	167, 758, 874
BstV2I	GAAGAC	1 cut(s)	446
BsuRI	GGCC	8 cut(s)	179, 250, 329, 425, 507, 1021, 1061, 1158
BtgI	CCRYGG	2 cut(s)	632, 699
BtrI	CACGTC	1 cut(s)	352
BtsCI	GGATG	3 cut(s)	165, 276, 496
Cac8I	GCNNGC	5 cut(s)	227, 826, 1138, 1160, 1164
CaiI	CAGNNNCTG	2 cut(s)	418, 1037
CciI	TCATGA	1 cut(s)	852
CfoI	GCGC	3 cut(s)	518, 751, 955
Cfr13I	GGNCC	6 cut(s)	249, 327, 328, 898, 1059, 1060
CseI	GACGC	2 cut(s)	391, 576
Csp6I	GTAC	3 cut(s)	145, 415, 569
CviAII	CATG	9 cut(s)	394, 509, 671, 700, 853, 970, 1208, 1301, 1315
CviQI	GTAC	3 cut(s)	145, 415, 569
DdeI	CTNAG	6 cut(s)	85, 519, 531, 537, 1110, 1296
DinI	GGCGCC	1 cut(s)	954
DpnI	GATC	2 cut(s)	644, 915
DpnII	GATC	2 cut(s)	642, 913
DraIII	CACNNNGTG	1 cut(s)	596
EaeI	YGGCCR	1 cut(s)	505
Eam1104I	CTCTTC	1 cut(s)	710
EarI	CTCTTC	1 cut(s)	710
Eco130I	CCWWGG	1 cut(s)	699
Eco147I	AGGCCT	1 cut(s)	1158
Eco24I	GRGCYC	2 cut(s)	331, 1063
Eco47I	GGWCC	1 cut(s)	898
Eco57I	CTGAAG	5 cut(s)	395, 458, 804, 891, 963
Eco88I	CYCGRG	1 cut(s)	209
EcoNI	CCTNNNNNAGG	1 cut(s)	173
EcoO109I	RGGNCCY	2 cut(s)	898, 1060
EcoRI	GAATTC	1 cut(s)	1265
EcoRII	CCWGG	3 cut(s)	387, 417, 1052
EcoT14I	CCWWGG	1 cut(s)	699
EcoT38I	GRGCYC	2 cut(s)	331, 1063
EgeI	GGCGCC	1 cut(s)	954
EheI	GGCGCC	1 cut(s)	954
ErhI	CCWWGG	1 cut(s)	699
Esp3I	CGTCTC	1 cut(s)	26
FaeI	CATG	9 cut(s)	397, 512, 674, 703, 856, 973, 1211, 1304, 1318
FaqI	GGGAC	3 cut(s)	203, 290, 884
FatI	CATG	9 cut(s)	393, 508, 670, 699, 852, 969, 1207, 1300, 1314
FauI	CCCGC	2 cut(s)	316, 763
FbaI	TGATCA	2 cut(s)	642, 913
FblI	GTMKAC	1 cut(s)	354
Fnu4HI	GCNGC	6 cut(s)	8, 156, 345, 505, 747, 863
FokI	GGATG	3 cut(s)	152, 263, 503
FriOI	GRGCYC	2 cut(s)	331, 1063
Fsp4HI	GCNGC	6 cut(s)	8, 156, 345, 505, 747, 863
FspBI	CTAG	1 cut(s)	1067
GlaI	GCGC	3 cut(s)	517, 750, 954
GluI	GCNGC	6 cut(s)	8, 156, 345, 505, 747, 863
GsuI	CTGGAG	1 cut(s)	440
HaeII	RGCGCY	2 cut(s)	519, 956
HaeIII	GGCC	8 cut(s)	179, 250, 329, 425, 507, 1021, 1061, 1158
HapII	CCGG	7 cut(s)	222, 252, 488, 657, 665, 742, 1147
HgaI	GACGC	2 cut(s)	391, 576
HhaI	GCGC	3 cut(s)	518, 751, 955
Hin1I	GRCGYC	3 cut(s)	300, 587, 953
Hin1II	CATG	9 cut(s)	397, 512, 674, 703, 856, 973, 1211, 1304, 1318
Hin6I	GCGC	3 cut(s)	516, 749, 953
HinP1I	GCGC	3 cut(s)	516, 749, 953
HincII	GTYRAC	1 cut(s)	355
HindII	GTYRAC	1 cut(s)	355
HinfI	GANTC	6 cut(s)	83, 533, 835, 920, 1173, 1251
HpaII	CCGG	7 cut(s)	222, 252, 488, 657, 665, 742, 1147
HphI	GGTGA	3 cut(s)	163, 582, 775
Hpy166II	GTNNAC	2 cut(s)	355, 472
Hpy188I	TCNGA	6 cut(s)	259, 438, 465, 538, 840, 871
Hpy188III	TCNNGA	4 cut(s)	488, 853, 917, 926
Hpy8I	GTNNAC	2 cut(s)	355, 472
Hpy99I	CGWCG	5 cut(s)	302, 305, 356, 407, 435
HpyCH4III	ACNGT	1 cut(s)	1043
HpyCH4IV	ACGT	3 cut(s)	300, 351, 558
HpyCH4V	TGCA	2 cut(s)	862, 1124
HpyF10VI	GCNNNNNNNGC	3 cut(s)	513, 617, 659
HpyF3I	CTNAG	6 cut(s)	85, 519, 531, 537, 1110, 1296
HpySE526I	ACGT	3 cut(s)	300, 351, 558
Hsp92I	GRCGYC	3 cut(s)	300, 587, 953
Hsp92II	CATG	9 cut(s)	397, 512, 674, 703, 856, 973, 1211, 1304, 1318
HspAI	GCGC	3 cut(s)	516, 749, 953
KasI	GGCGCC	1 cut(s)	952
KflI	GGGWCCC	1 cut(s)	898
Kpn2I	TCCGGA	1 cut(s)	487
Ksp22I	TGATCA	2 cut(s)	642, 913
Kzo9I	GATC	2 cut(s)	642, 913
LguI	GCTCTTC	1 cut(s)	710
LmnI	GCTCC	6 cut(s)	4, 15, 260, 389, 465, 1209
Lsp1109I	GCAGC	3 cut(s)	167, 758, 874
LweI	GCATC	2 cut(s)	1123, 1133
MaeI	CTAG	1 cut(s)	1067
MaeII	ACGT	3 cut(s)	300, 351, 558
MaeIII	GTNAC	3 cut(s)	588, 1149, 1303
MalI	GATC	2 cut(s)	644, 915
MboI	GATC	2 cut(s)	642, 913
MboII	GAAGA	5 cut(s)	83, 451, 727, 956, 1082
MfeI	CAATTG	1 cut(s)	1167
MhlI	GDGCHC	6 cut(s)	318, 331, 459, 632, 664, 1063
MluCI	AATT	5 cut(s)	856, 936, 1167, 1257, 1265
Mly113I	GGCGCC	1 cut(s)	953
MlyI	GAGTC	4 cut(s)	77, 542, 844, 929
MmeI	TCCRAC	2 cut(s)	282, 863
MroI	TCCGGA	1 cut(s)	487
MseI	TTAA	1 cut(s)	804
MspI	CCGG	7 cut(s)	222, 252, 488, 657, 665, 742, 1147
MspR9I	CCNGG	8 cut(s)	222, 252, 389, 419, 658, 666, 743, 1054
MunI	CAATTG	1 cut(s)	1167
MvaI	CCWGG	3 cut(s)	389, 419, 1054
MwoI	GCNNNNNNNGC	3 cut(s)	513, 617, 659
NarI	GGCGCC	1 cut(s)	953
NciI	CCSGG	5 cut(s)	222, 252, 658, 666, 743
NcoI	CCATGG	1 cut(s)	699
NdeII	GATC	2 cut(s)	642, 913
NlaIII	CATG	9 cut(s)	397, 512, 674, 703, 856, 973, 1211, 1304, 1318
NmeAIII	GCCGAG	1 cut(s)	337
NmuCI	GTSAC	2 cut(s)	588, 1303
NspI	RCATGY	1 cut(s)	1304
PaeR7I	CTCGAG	1 cut(s)	209
PagI	TCATGA	1 cut(s)	852
PceI	AGGCCT	1 cut(s)	1158
PciSI	GCTCTTC	1 cut(s)	710
PcsI	WCGNNNNNNNCGW	2 cut(s)	309, 348
PfeI	GAWTC	2 cut(s)	1173, 1251
PflFI	GACNNNGTC	1 cut(s)	302
PflMI	CCANNNNNTGG	1 cut(s)	1169
PkrI	GCNGC	6 cut(s)	9, 157, 346, 506, 748, 864
PleI	GAGTC	4 cut(s)	77, 541, 843, 928
PluTI	GGCGCC	1 cut(s)	956
PpsI	GAGTC	4 cut(s)	77, 541, 843, 928
PpuMI	RGGWCCY	1 cut(s)	898
Psp1406I	AACGTT	1 cut(s)	558
Psp5II	RGGWCCY	1 cut(s)	898
Psp6I	CCWGG	3 cut(s)	387, 417, 1052
PspGI	CCWGG	3 cut(s)	387, 417, 1052
PspOMI	GGGCCC	2 cut(s)	327, 1059
PspPI	GGNCC	6 cut(s)	249, 327, 328, 898, 1059, 1060
PspPPI	RGGWCCY	1 cut(s)	898
PspXI	VCTCGAGB	1 cut(s)	209
PstNI	CAGNNNCTG	2 cut(s)	418, 1037
PsyI	GACNNNGTC	1 cut(s)	302
RsaI	GTAC	3 cut(s)	146, 416, 570
RsaNI	GTAC	3 cut(s)	145, 415, 569
SalI	GTCGAC	1 cut(s)	353
SapI	GCTCTTC	1 cut(s)	710
SaqAI	TTAA	1 cut(s)	804
SatI	GCNGC	6 cut(s)	8, 156, 345, 505, 747, 863
Sau3AI	GATC	2 cut(s)	642, 913
Sau96I	GGNCC	6 cut(s)	249, 327, 328, 898, 1059, 1060
ScaI	AGTACT	1 cut(s)	570
SchI	GAGTC	4 cut(s)	77, 542, 844, 929
ScrFI	CCNGG	8 cut(s)	222, 252, 389, 419, 658, 666, 743, 1054
SduI	GDGCHC	6 cut(s)	318, 331, 459, 632, 664, 1063
SfaNI	GCATC	2 cut(s)	1123, 1133
SfcI	CTRYAG	1 cut(s)	604
SfoI	GGCGCC	1 cut(s)	954
Sfr274I	CTCGAG	1 cut(s)	209
SinI	GGWCC	1 cut(s)	898
SlaI	CTCGAG	1 cut(s)	209
SmlI	CTYRAG	1 cut(s)	209
SmoI	CTYRAG	1 cut(s)	209
Sse9I	AATT	5 cut(s)	856, 936, 1167, 1257, 1265
SseBI	AGGCCT	1 cut(s)	1158
SsiI	CCGC	7 cut(s)	66, 126, 309, 345, 504, 620, 770
SspDI	GGCGCC	1 cut(s)	952
SspMI	CTAG	1 cut(s)	1067
StuI	AGGCCT	1 cut(s)	1158
StyD4I	CCNGG	8 cut(s)	220, 250, 387, 417, 656, 664, 741, 1052
StyI	CCWWGG	1 cut(s)	699
TaaI	ACNGT	1 cut(s)	1043
TaiI	ACGT	3 cut(s)	303, 354, 561
TaqI	TCGA	2 cut(s)	210, 354
TasI	AATT	5 cut(s)	856, 936, 1167, 1257, 1265
TatI	WGTACW	1 cut(s)	568
TauI	GCSGC	2 cut(s)	347, 507
TfiI	GAWTC	2 cut(s)	1173, 1251
Tru1I	TTAA	1 cut(s)	804
Tru9I	TTAA	1 cut(s)	804
TseFI	GTSAC	2 cut(s)	588, 1303
TseI	GCWGC	4 cut(s)	7, 155, 746, 862
Tsp45I	GTSAC	2 cut(s)	588, 1303
TspDTI	ATGAA	7 cut(s)	154, 410, 591, 869, 1258, 1275, 1278
TspGWI	ACGGA	2 cut(s)	8, 1107
Tth111I	GACNNNGTC	1 cut(s)	302
Van91I	CCANNNNNTGG	1 cut(s)	1169
VpaK11BI	GGWCC	1 cut(s)	898
XagI	CCTNNNNNAGG	1 cut(s)	173
XapI	RAATTY	3 cut(s)	856, 1257, 1265
XceI	RCATGY	1 cut(s)	1304
XhoI	CTCGAG	1 cut(s)	209
XmiI	GTMKAC	1 cut(s)	354
XspI	CTAG	1 cut(s)	1067
ZraI	GACGTC	1 cut(s)	301
ZrmI	AGTACT	1 cut(s)	570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.