Rh2BG327100

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004417
Physical Location & Seq
Forward (+)
42228824 .. 42235727
6904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG327100.1

Sequence Viewer

Length: 702 bp
ATGGCCGTGGTAGAATATCGCCGACCGAGGAGACAGAGTGCCCCAGATCCACTTTCTCTCTCCTCCATCAAATGGCCCGACTCCGACCCGACTCTCTCCTCCATCCTCCTCAACTCCTACACCGACGTCGTCCTGGCCGAGCACAGAAAGTGGCCCCACCATCCTTTCTCGGCCCTCGTCGACCCGAGTGGGAAAATCTACGCCAGAGGCTCCCAGGACATGAAGTGCAACGGGCTTCAGTACCTAGAGGCCATTCGACGGCTTAAGGCTTCTGGGTTTAAGCAGACTCGGACTGTTTACTTGTCTTTCGTTCCTGATGAGGAGATTGGTGGTCATGATGGCGCTGAGAAGTTTGCTGAATCTGATATTTTCAAAGCCTTGAACGTTGGGATAGTTCTCGATGAAGGGTTGGCCTCGCCGAGCGAGCATTATAGGGCTTTCTATGCGGAGAGGTGCCCATGGTGGCTGATCATCAAGGCTACTGGGGCTCCTGGTCATGGGGCTAAGCTGTATGACAACACTGCCATGGAGAATATATTGAAGAGCATTGAGAGTGTGAGGAGATTTCGTGCTTCACAGTTTGATTTGGTGAAGGCGGGTTTGAAGGCTGAAGGGGAGGTCGTCTCTGTCAATATGGCATTTTTGAAGGCTGGCACACCCTGTCCGACTGTTATCAATATTCTGCAATGCTTAATAGAATAA

Protein Analysis

233

Amino Acids

25.91

Weight (kDa)

7.69

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 37 - 131 2.2e-19 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 453
AccB7I CCANNNNNTGG 1 cut(s) 72
AccI GTMKAC 1 cut(s) 180
AciI CCGC 2 cut(s) 446, 596
AclI AACGTT 1 cut(s) 384
AclWI GGATC 1 cut(s) 41
AcoI YGGCCR 2 cut(s) 3, 135
AcuI CTGAAG 2 cut(s) 221, 630
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 3 cut(s) 72, 258, 497
AflII CTTAAG 1 cut(s) 263
AgsI TTSAA 5 cut(s) 373, 382, 541, 604, 646
AjnI CCWGG 3 cut(s) 132, 213, 490
AluBI AGCT 1 cut(s) 508
AluI AGCT 1 cut(s) 508
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 25, 628
AlwI GGATC 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 7 cut(s) 3, 74, 135, 152, 171, 249, 411
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 3 cut(s) 75, 153, 172
AsuHPI GGTGA 1 cut(s) 601
AvaI CYCGRG 1 cut(s) 184
BaeGI GKGCMC 2 cut(s) 43, 458
BanI GGYRCC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 144
BccI CCATC 4 cut(s) 74, 110, 168, 332
BceAI ACGGC 1 cut(s) 275
BciT130I CCWGG 3 cut(s) 134, 215, 492
BclI TGATCA 1 cut(s) 468
BcoDI GTCTC 2 cut(s) 25, 628
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 345
BfrI CTTAAG 1 cut(s) 263
BlpI GCTNAGC 1 cut(s) 504
Bme1390I CCNGG 3 cut(s) 134, 215, 492
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 3 cut(s) 75, 153, 172
BmiI GGNNCC 4 cut(s) 155, 211, 455, 489
BmrFI CCNGG 3 cut(s) 134, 215, 492
BmrI ACTGGG 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 492
BplI GAGNNNNNCTC 2 cut(s) 608, 640
Bpu1102I GCTNAGC 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 5 cut(s) 6, 26, 213, 458, 525
BsaXI ACNNNNNCTCC 6 cut(s) 22, 52, 472, 502, 553, 583
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 258, 497
Bse1I ACTGG 1 cut(s) 487
Bse3DI GCAATG 1 cut(s) 692
BseBI CCWGG 3 cut(s) 134, 215, 492
BseDI CCNNGG 5 cut(s) 6, 26, 213, 458, 525
BseGI GGATG 2 cut(s) 102, 160
BseLI CCNNNNNNNGG 3 cut(s) 72, 258, 497
BseMI GCAATG 1 cut(s) 692
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 6 cut(s) 43, 52, 88, 98, 335, 574
BseSI GKGCMC 2 cut(s) 43, 458
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 7 cut(s) 5, 76, 137, 154, 173, 251, 413
BshNI GGYRCC 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 184
BslI CCNNNNNNNGG 3 cut(s) 72, 258, 497
BsmAI GTCTC 2 cut(s) 25, 628
BsmBI CGTCTC 1 cut(s) 628
BsnI GGCC 7 cut(s) 5, 76, 137, 154, 173, 251, 413
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 4 cut(s) 43, 144, 458, 490
Bsp143I GATC 2 cut(s) 46, 468
Bsp1720I GCTNAGC 1 cut(s) 504
Bsp19I CCATGG 2 cut(s) 458, 525
BspACI CCGC 2 cut(s) 446, 596
BspANI GGCC 7 cut(s) 5, 76, 137, 154, 173, 251, 413
BspCNI CTCAG 1 cut(s) 337
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 4 cut(s) 155, 211, 455, 489
BspPI GGATC 1 cut(s) 41
BspQI GCTCTTC 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 453
BspTI CTTAAG 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 692
BsrI ACTGG 1 cut(s) 487
BssECI CCNNGG 5 cut(s) 6, 26, 213, 458, 525
BssMI GATC 2 cut(s) 46, 468
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 458, 525
Bst2UI CCWGG 3 cut(s) 134, 215, 492
Bst4CI ACNGT 3 cut(s) 295, 579, 670
Bst6I CTCTTC 1 cut(s) 536
BstACI GRCGYC 1 cut(s) 126
BstAFI CTTAAG 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 425, 652
BstDEI CTNAG 2 cut(s) 345, 504
BstDSI CCRYGG 3 cut(s) 6, 458, 525
BstF5I GGATG 2 cut(s) 102, 160
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 2 cut(s) 49, 471
BstMAI GTCTC 2 cut(s) 25, 628
BstMBI GATC 2 cut(s) 46, 468
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 3 cut(s) 424, 443, 485
BstNI CCWGG 3 cut(s) 134, 215, 492
BstSCI CCNGG 3 cut(s) 132, 213, 490
BstSLI GKGCMC 2 cut(s) 43, 458
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 7 cut(s) 5, 76, 137, 154, 173, 251, 413
BtgI CCRYGG 3 cut(s) 6, 458, 525
BtsCI GGATG 2 cut(s) 102, 160
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 2 cut(s) 425, 652
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 3 cut(s) 75, 153, 172
Csp6I GTAC 1 cut(s) 241
CviAII CATG 5 cut(s) 220, 335, 459, 497, 526
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 2 cut(s) 345, 504
DpnI GATC 2 cut(s) 48, 470
DpnII GATC 2 cut(s) 46, 468
EaeI YGGCCR 2 cut(s) 3, 135
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
Eco130I CCWWGG 2 cut(s) 458, 525
Eco24I GRGCYC 1 cut(s) 490
Eco57I CTGAAG 2 cut(s) 221, 630
Eco88I CYCGRG 1 cut(s) 184
EcoRII CCWGG 3 cut(s) 132, 213, 490
EcoT14I CCWWGG 2 cut(s) 458, 525
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 2 cut(s) 458, 525
Esp3I CGTCTC 1 cut(s) 628
FaeI CATG 5 cut(s) 223, 338, 462, 500, 529
FatI CATG 5 cut(s) 219, 334, 458, 496, 525
FauI CCCGC 1 cut(s) 589
FbaI TGATCA 1 cut(s) 468
FblI GTMKAC 1 cut(s) 180
FokI GGATG 2 cut(s) 89, 147
FriOI GRGCYC 1 cut(s) 490
FspBI CTAG 1 cut(s) 245
GlaI GCGC 1 cut(s) 343
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 7 cut(s) 5, 76, 137, 154, 173, 251, 413
HhaI GCGC 1 cut(s) 344
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 5 cut(s) 223, 338, 462, 500, 529
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 4 cut(s) 80, 91, 286, 359
HphI GGTGA 1 cut(s) 601
Hpy166II GTNNAC 2 cut(s) 181, 298
Hpy188I TCNGA 4 cut(s) 85, 291, 364, 666
Hpy188III TCNNGA 3 cut(s) 314, 335, 398
Hpy8I GTNNAC 2 cut(s) 181, 298
Hpy99I CGWCG 4 cut(s) 128, 131, 182, 261
HpyAV CCTTC 5 cut(s) 398, 586, 598, 605, 640
HpyCH4III ACNGT 3 cut(s) 295, 579, 670
HpyCH4IV ACGT 2 cut(s) 126, 384
HpyCH4V TGCA 2 cut(s) 228, 685
HpyF10VI GCNNNNNNNGC 3 cut(s) 424, 443, 485
HpyF3I CTNAG 2 cut(s) 345, 504
HpySE526I ACGT 2 cut(s) 126, 384
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 5 cut(s) 223, 338, 462, 500, 529
HspAI GCGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 468
Kzo9I GATC 2 cut(s) 46, 468
LguI GCTCTTC 1 cut(s) 536
LmnI GCTCC 2 cut(s) 215, 493
MaeI CTAG 1 cut(s) 245
MaeII ACGT 2 cut(s) 126, 384
MalI GATC 2 cut(s) 48, 470
MboI GATC 2 cut(s) 46, 468
MboII GAAGA 1 cut(s) 553
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 4 cut(s) 43, 144, 458, 490
MlyI GAGTC 3 cut(s) 74, 85, 280
MmeI TCCRAC 2 cut(s) 108, 689
MseI TTAA 3 cut(s) 264, 279, 692
MslI CAYNNNNRTG 1 cut(s) 524
MspCI CTTAAG 1 cut(s) 263
MspR9I CCNGG 3 cut(s) 134, 215, 492
MvaI CCWGG 3 cut(s) 134, 215, 492
MwoI GCNNNNNNNGC 3 cut(s) 424, 443, 485
NcoI CCATGG 2 cut(s) 458, 525
NdeII GATC 2 cut(s) 46, 468
NlaIII CATG 5 cut(s) 223, 338, 462, 500, 529
NlaIV GGNNCC 4 cut(s) 155, 211, 455, 489
NmeAIII GCCGAG 3 cut(s) 149, 163, 444
PagI TCATGA 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PfeI GAWTC 1 cut(s) 359
PflFI GACNNNGTC 1 cut(s) 128
PflMI CCANNNNNTGG 1 cut(s) 72
PleI GAGTC 3 cut(s) 74, 85, 280
PpsI GAGTC 3 cut(s) 74, 85, 280
Psp1406I AACGTT 1 cut(s) 384
Psp6I CCWGG 3 cut(s) 132, 213, 490
PspGI CCWGG 3 cut(s) 132, 213, 490
PspN4I GGNNCC 4 cut(s) 155, 211, 455, 489
PspPI GGNCC 3 cut(s) 75, 153, 172
PsuI RGATCY 1 cut(s) 46
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 524
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 536
SaqAI TTAA 3 cut(s) 264, 279, 692
Sau3AI GATC 2 cut(s) 46, 468
Sau96I GGNCC 3 cut(s) 75, 153, 172
SchI GAGTC 3 cut(s) 74, 85, 280
ScrFI CCNGG 3 cut(s) 134, 215, 492
SduI GDGCHC 4 cut(s) 43, 144, 458, 490
SetI ASST 6 cut(s) 129, 246, 387, 455, 510, 621
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SsiI CCGC 2 cut(s) 446, 596
SspI AATATT 1 cut(s) 679
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 3 cut(s) 132, 213, 490
StyI CCWWGG 2 cut(s) 458, 525
TaaI ACNGT 3 cut(s) 295, 579, 670
TaiI ACGT 2 cut(s) 129, 387
TaqI TCGA 3 cut(s) 180, 256, 399
TaqII GACCGA 1 cut(s) 40
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 3 cut(s) 264, 279, 692
Tru9I TTAA 3 cut(s) 264, 279, 692
TscAI CASTG 1 cut(s) 526
TspDTI ATGAA 2 cut(s) 236, 417
TspRI CASTG 1 cut(s) 526
Tth111I GACNNNGTC 1 cut(s) 128
Van91I CCANNNNNTGG 1 cut(s) 72
Vha464I CTTAAG 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 245
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.