Rw3G017710

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004417
Physical Location & Seq
Forward (+)
19436969 .. 19440332
3364 bp
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UTR
Exon/CDS
Intron
Rw3G017710.1

Sequence Viewer

Length: 615 bp
ATGAAGTGCATCGGGCTTCAATACTTGGAGGCCATTCGACGGCTTAAGGCTTCTGGGTTTAAGCCGACTCGGACTGTTTACTTATCTTTCGTTCCTGATGAGGAGATTGGTGGTCATGATGGCGCTGAGAAGTTTGCTGAATCTGATATTTTCAAAGCCCTGAACGTTGGGATAGTTCTCGATGAAGGGTTGGCCTCGCCGAGTGAGCATTATAGGGCTTTCTATGCGGAGAGGTGCCCGTGGTGGCTGATCATCAAGGCTACTGGGGCTCCTGGTCATGGGGCTAAGCTGTATGACAACACTGCCGTGGAGAATATATTGAAGAGCATTGAGAGTGCGGGTTTGAAGGCTGAAGGGGAGGTCGTCTCTGTCAATATGGCATTTTTGAAGGCTGGCACACCCTCTCCAACTATAAGGGTCATGAACTTGTACTATGTACTTGATAAGAAGTTGTGTGAAAATGTTACCTCACGTCATCAACAGTCTATGTTCAAACCTAATGTTCTCGATATATATTTCTATATGCAGTGTCCCGTTGTCACTGATTTGAATTACAATTTAGAAACTTCTGAATGGTGCCTAGTTTATGAATATATAATGCTAGTGTATTCTTAG

Protein Analysis

204

Amino Acids

23.01

Weight (kDa)

5.61

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 1 - 59 7.1e-10 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 234, 576
AciI CCGC 2 cut(s) 227, 338
AclI AACGTT 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 2 cut(s) 431, 438
AfiI CCNNNNNNNGG 2 cut(s) 39, 278
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 7 cut(s) 20, 154, 322, 346, 388, 493, 550
AjiI CACGTC 1 cut(s) 473
AjnI CCWGG 1 cut(s) 271
AleI CACNNNNGTG 1 cut(s) 305
AluBI AGCT 1 cut(s) 289
AluI AGCT 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 370
AoxI GGCC 2 cut(s) 30, 192
AspLEI GCGC 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 239
BanI GGYRCC 2 cut(s) 234, 576
BanII GRGCYC 1 cut(s) 271
BccI CCATC 1 cut(s) 113
BceAI ACGGC 2 cut(s) 56, 290
BciT130I CCWGG 1 cut(s) 273
BclI TGATCA 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 2 cut(s) 581, 602
BfoI RGCGCY 1 cut(s) 126
BfrI CTTAAG 1 cut(s) 44
BlpI GCTNAGC 1 cut(s) 285
Bme1390I CCNGG 1 cut(s) 273
BmgBI CACGTC 1 cut(s) 473
BmiI GGNNCC 3 cut(s) 236, 270, 578
BmrFI CCNGG 1 cut(s) 273
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 1 cut(s) 18
BmuI ACTGGG 1 cut(s) 273
BplI GAGNNNNNCTC 2 cut(s) 350, 382
Bpu1102I GCTNAGC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 239, 306
BsaXI ACNNNNNCTCC 4 cut(s) 253, 283, 388, 418
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 278
Bse1I ACTGG 1 cut(s) 268
BseBI CCWGG 1 cut(s) 273
BseDI CCNNGG 2 cut(s) 239, 306
BseLI CCNNNNNNNGG 2 cut(s) 39, 278
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 239
BshFI GGCC 2 cut(s) 32, 194
BshNI GGYRCC 2 cut(s) 234, 576
BslFI GGGAC 1 cut(s) 516
BslI CCNNNNNNNGG 2 cut(s) 39, 278
BsmAI GTCTC 1 cut(s) 370
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 516
BsnI GGCC 2 cut(s) 32, 194
Bsp1286I GDGCHC 2 cut(s) 239, 271
Bsp143I GATC 1 cut(s) 249
Bsp1720I GCTNAGC 1 cut(s) 285
BspACI CCGC 2 cut(s) 227, 338
BspANI GGCC 2 cut(s) 32, 194
BspCNI CTCAG 1 cut(s) 118
BspHI TCATGA 2 cut(s) 115, 420
BspLI GGNNCC 3 cut(s) 236, 270, 578
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 2 cut(s) 234, 576
BspTI CTTAAG 1 cut(s) 44
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 239, 306
BssMI GATC 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 2 cut(s) 76, 483
Bst6I CTCTTC 1 cut(s) 317
BstAFI CTTAAG 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 3 cut(s) 126, 285, 612
BstDSI CCRYGG 2 cut(s) 239, 306
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 1 cut(s) 252
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 1 cut(s) 249
BstMWI GCNNNNNNNGC 3 cut(s) 205, 224, 266
BstNI CCWGG 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 271
BstSLI GKGCMC 1 cut(s) 239
BsuRI GGCC 2 cut(s) 32, 194
BtgI CCRYGG 2 cut(s) 239, 306
BtrI CACGTC 1 cut(s) 473
BtsI GCAGTG 2 cut(s) 300, 533
BtsIMutI CAGTG 3 cut(s) 300, 533, 540
Cac8I GCNNGC 1 cut(s) 394
CciI TCATGA 2 cut(s) 115, 420
CfoI GCGC 1 cut(s) 125
Csp6I GTAC 2 cut(s) 430, 437
CviAII CATG 3 cut(s) 116, 278, 421
CviQI GTAC 2 cut(s) 430, 437
DdeI CTNAG 3 cut(s) 126, 285, 612
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 3 cut(s) 119, 281, 424
FaqI GGGAC 1 cut(s) 516
FatI CATG 3 cut(s) 115, 277, 420
FauI CCCGC 1 cut(s) 331
FbaI TGATCA 1 cut(s) 249
FriOI GRGCYC 1 cut(s) 271
FspBI CTAG 2 cut(s) 581, 602
GlaI GCGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 2 cut(s) 32, 194
HhaI GCGC 1 cut(s) 125
Hin1II CATG 3 cut(s) 119, 281, 424
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HinfI GANTC 2 cut(s) 67, 140
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 3 cut(s) 72, 145, 571
Hpy188III TCNNGA 5 cut(s) 95, 116, 179, 421, 506
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 4 cut(s) 179, 340, 347, 382
HpyCH4III ACNGT 2 cut(s) 76, 483
HpyCH4IV ACGT 2 cut(s) 165, 472
HpyCH4V TGCA 2 cut(s) 9, 526
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 224, 266
HpyF3I CTNAG 3 cut(s) 126, 285, 612
HpySE526I ACGT 2 cut(s) 165, 472
Hsp92II CATG 3 cut(s) 119, 281, 424
HspAI GCGC 1 cut(s) 123
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 1 cut(s) 249
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 7 cut(s) 39, 108, 173, 249, 258, 285, 378
LweI GCATC 1 cut(s) 18
MaeI CTAG 2 cut(s) 581, 602
MaeII ACGT 2 cut(s) 165, 472
MaeIII GTNAC 2 cut(s) 463, 538
MalI GATC 1 cut(s) 251
MboI GATC 1 cut(s) 249
MboII GAAGA 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 239, 271
MluCI AATT 2 cut(s) 550, 556
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 7 cut(s) 22, 94, 205, 225, 352, 412, 478
MseI TTAA 2 cut(s) 45, 60
MslI CAYNNNNRTG 1 cut(s) 305
MspCI CTTAAG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 273
MvaI CCWGG 1 cut(s) 273
MwoI GCNNNNNNNGC 3 cut(s) 205, 224, 266
NdeII GATC 1 cut(s) 249
NlaIII CATG 3 cut(s) 119, 281, 424
NlaIV GGNNCC 3 cut(s) 236, 270, 578
NmeAIII GCCGAG 1 cut(s) 225
NmuCI GTSAC 1 cut(s) 538
OliI CACNNNNGTG 1 cut(s) 305
PagI TCATGA 2 cut(s) 115, 420
PciSI GCTCTTC 1 cut(s) 317
PfeI GAWTC 1 cut(s) 140
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Psp1406I AACGTT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 3 cut(s) 236, 270, 578
RsaI GTAC 2 cut(s) 431, 438
RsaNI GTAC 2 cut(s) 430, 437
RseI CAYNNNNRTG 1 cut(s) 305
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 45, 60
Sau3AI GATC 1 cut(s) 249
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 1 cut(s) 273
SduI GDGCHC 2 cut(s) 239, 271
SetI ASST 7 cut(s) 168, 236, 291, 363, 470, 475, 499
SfaNI GCATC 1 cut(s) 18
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 2 cut(s) 550, 556
SsiI CCGC 2 cut(s) 227, 338
SspMI CTAG 2 cut(s) 581, 602
StyD4I CCNGG 1 cut(s) 271
TaaI ACNGT 2 cut(s) 76, 483
TaiI ACGT 2 cut(s) 168, 475
TaqI TCGA 3 cut(s) 37, 180, 507
TasI AATT 2 cut(s) 550, 556
TatI WGTACW 2 cut(s) 429, 436
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 2 cut(s) 45, 60
Tru9I TTAA 2 cut(s) 45, 60
TscAI CASTG 3 cut(s) 307, 533, 547
TseFI GTSAC 1 cut(s) 538
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 4 cut(s) 17, 198, 437, 603
TspRI CASTG 3 cut(s) 307, 533, 547
Vha464I CTTAAG 1 cut(s) 44
XspI CTAG 2 cut(s) 581, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.