Rw7G031480

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004417
Physical Location & Seq
Forward (+)
45120857 .. 45124208
3352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G031480.1

Sequence Viewer

Length: 573 bp
ATGAAGTGCGTCAGACTTCAGTACCTGGAGGCCATTCGACGGCTTAAGGCTTCTGGGTTTAAGCCGACTTGGACTGTTTACTTGTCTTTCGTTCCTGATGAGGAGATTGGTGGTCATGATGGCGCTGAGAAGTTTGCTGAATCTGATATTTTCAAAGCCTTGAATGTTGGGATAGTTCTCGATGAAGAGGTGCCCGTGGTGGCTGATCATAAGGCTACTGGGGCTCCTGGTCATGGGGCTAAGCTGTATGACAACACTGCCATGGAGAATATATTGAAGAGCATTGAGAGTGTGAGGAGATTTCGTGCTTCGCAGTTTGATTTGGTGAAGGTGGGTTTGAAGGCTGAAGGGGAGGTCGTCTCTGTCAATATGGCATTTTTGAAGGCTGGCACACCCTCTCCGACTGTGAGTTGTGATTGGATATGGGTTCCCTTAAGTTTGTCTATGTTCAAACTTAATGTTCTCGATATATATTTCTATATGCAGTGTCCCGTTGTCACTGATTTGAATTACAATTTAGAAACTTCTGAATGGTGCCTAGTTTATGAATATATAATGCCAGTGTATTCTTAG

Protein Analysis

190

Amino Acids

21.36

Weight (kDa)

5.11

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 1 - 58 7.4e-08 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 190, 534
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 2 cut(s) 39, 233
AflII CTTAAG 2 cut(s) 44, 433
AgsI TTSAA 7 cut(s) 154, 163, 277, 340, 382, 451, 508
AjnI CCWGG 2 cut(s) 24, 226
AluBI AGCT 1 cut(s) 244
AluI AGCT 1 cut(s) 244
Alw26I GTCTC 1 cut(s) 364
AlwNI CAGNNNCTG 1 cut(s) 25
AoxI GGCC 1 cut(s) 30
AspLEI GCGC 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 195
BaeI ACNNNNGTAYC 1 cut(s) 38
BanI GGYRCC 2 cut(s) 190, 534
BanII GRGCYC 1 cut(s) 226
BccI CCATC 1 cut(s) 113
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 2 cut(s) 26, 228
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 364
BfaI CTAG 1 cut(s) 539
BfoI RGCGCY 1 cut(s) 126
BfrI CTTAAG 2 cut(s) 44, 433
BlpI GCTNAGC 1 cut(s) 240
Bme1390I CCNGG 2 cut(s) 26, 228
BmiI GGNNCC 4 cut(s) 192, 225, 429, 536
BmrFI CCNGG 2 cut(s) 26, 228
BmrI ACTGGG 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 228
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 1 cut(s) 47
Bpu1102I GCTNAGC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 195, 261
BsaXI ACNNNNNCTCC 6 cut(s) 208, 238, 289, 319, 382, 412
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 233
Bse1I ACTGG 2 cut(s) 223, 560
BseBI CCWGG 2 cut(s) 26, 228
BseDI CCNNGG 2 cut(s) 195, 261
BseLI CCNNNNNNNGG 2 cut(s) 39, 233
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 2 cut(s) 223, 560
BseRI GAGGAG 2 cut(s) 116, 310
BseSI GKGCMC 1 cut(s) 195
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 2 cut(s) 190, 534
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 2 cut(s) 39, 233
BsmAI GTCTC 1 cut(s) 364
BsmBI CGTCTC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 1 cut(s) 32
Bsp1286I GDGCHC 2 cut(s) 195, 226
Bsp143I GATC 1 cut(s) 205
Bsp1720I GCTNAGC 1 cut(s) 240
Bsp19I CCATGG 1 cut(s) 261
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 4 cut(s) 192, 225, 429, 536
BspQI GCTCTTC 1 cut(s) 272
BspT107I GGYRCC 2 cut(s) 190, 534
BspTI CTTAAG 2 cut(s) 44, 433
BsrI ACTGG 2 cut(s) 223, 560
BssECI CCNNGG 2 cut(s) 195, 261
BssMI GATC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 2 cut(s) 26, 228
Bst4CI ACNGT 2 cut(s) 76, 406
Bst6I CTCTTC 2 cut(s) 180, 272
BstAFI CTTAAG 2 cut(s) 44, 433
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 3 cut(s) 126, 240, 570
BstDSI CCRYGG 2 cut(s) 195, 261
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstNI CCWGG 2 cut(s) 26, 228
BstSCI CCNGG 2 cut(s) 24, 226
BstSLI GKGCMC 1 cut(s) 195
BsuRI GGCC 1 cut(s) 32
BtgI CCRYGG 2 cut(s) 195, 261
BtsI GCAGTG 2 cut(s) 255, 491
BtsIMutI CAGTG 4 cut(s) 255, 491, 498, 567
Cac8I GCNNGC 1 cut(s) 388
CaiI CAGNNNCTG 1 cut(s) 25
CciI TCATGA 1 cut(s) 115
CfoI GCGC 1 cut(s) 125
Csp6I GTAC 1 cut(s) 22
CviAII CATG 3 cut(s) 116, 233, 262
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 3 cut(s) 126, 240, 570
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
Eam1104I CTCTTC 2 cut(s) 180, 272
EarI CTCTTC 2 cut(s) 180, 272
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 366
EcoRII CCWGG 2 cut(s) 24, 226
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 3 cut(s) 119, 236, 265
FaqI GGGAC 1 cut(s) 474
FatI CATG 3 cut(s) 115, 232, 261
FbaI TGATCA 1 cut(s) 205
FriOI GRGCYC 1 cut(s) 226
FspBI CTAG 1 cut(s) 539
GlaI GCGC 1 cut(s) 124
GsuI CTGGAG 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 1 cut(s) 32
HhaI GCGC 1 cut(s) 125
Hin1II CATG 3 cut(s) 119, 236, 265
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HinfI GANTC 1 cut(s) 140
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 4 cut(s) 14, 145, 402, 529
Hpy188III TCNNGA 4 cut(s) 95, 116, 179, 464
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 4 cut(s) 322, 334, 341, 376
HpyCH4III ACNGT 2 cut(s) 76, 406
HpyCH4V TGCA 1 cut(s) 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpyF3I CTNAG 3 cut(s) 126, 240, 570
Hsp92II CATG 3 cut(s) 119, 236, 265
HspAI GCGC 1 cut(s) 123
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 1 cut(s) 205
LguI GCTCTTC 1 cut(s) 272
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 8 cut(s) 11, 38, 39, 108, 204, 213, 240, 372
MaeI CTAG 1 cut(s) 539
MaeIII GTNAC 1 cut(s) 496
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 2 cut(s) 197, 289
MhlI GDGCHC 2 cut(s) 195, 226
MluCI AATT 2 cut(s) 508, 514
MmeI TCCRAC 1 cut(s) 425
MnlI CCTC 6 cut(s) 22, 94, 181, 288, 346, 406
MseI TTAA 4 cut(s) 45, 60, 434, 456
MslI CAYNNNNRTG 1 cut(s) 260
MspCI CTTAAG 2 cut(s) 44, 433
MspR9I CCNGG 2 cut(s) 26, 228
MvaI CCWGG 2 cut(s) 26, 228
MwoI GCNNNNNNNGC 1 cut(s) 221
NcoI CCATGG 1 cut(s) 261
NdeII GATC 1 cut(s) 205
NlaIII CATG 3 cut(s) 119, 236, 265
NlaIV GGNNCC 4 cut(s) 192, 225, 429, 536
NmuCI GTSAC 1 cut(s) 496
PagI TCATGA 1 cut(s) 115
PciSI GCTCTTC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 24, 226
PspGI CCWGG 2 cut(s) 24, 226
PspN4I GGNNCC 4 cut(s) 192, 225, 429, 536
PstNI CAGNNNCTG 1 cut(s) 25
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 272
SaqAI TTAA 4 cut(s) 45, 60, 434, 456
Sau3AI GATC 1 cut(s) 205
ScrFI CCNGG 2 cut(s) 26, 228
SduI GDGCHC 2 cut(s) 195, 226
SetI ASST 5 cut(s) 27, 192, 246, 333, 357
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 2 cut(s) 44, 433
SmoI CTYRAG 2 cut(s) 44, 433
Sse9I AATT 2 cut(s) 508, 514
SspMI CTAG 1 cut(s) 539
StyD4I CCNGG 2 cut(s) 24, 226
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 2 cut(s) 76, 406
TaqI TCGA 3 cut(s) 37, 180, 465
TasI AATT 2 cut(s) 508, 514
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 4 cut(s) 45, 60, 434, 456
Tru9I TTAA 4 cut(s) 45, 60, 434, 456
TscAI CASTG 4 cut(s) 262, 491, 505, 567
TseFI GTSAC 1 cut(s) 496
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 3 cut(s) 17, 198, 561
TspRI CASTG 4 cut(s) 262, 491, 505, 567
Vha464I CTTAAG 2 cut(s) 44, 433
XspI CTAG 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.