Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004417

Physical Location & Seq

Genomic Coordinates Reverse (-)

22870441 .. 22874477

Gene Structure 4037 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1142800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGGAGCTTCGTCTTCGTCTCTGCATAATCTCTCTGCTACTGCTCTCATCAAAATCAACGGCCATACAATCCGACGACCCGATAATCTCACGGTTTCAACGATACCTCCAAATCAACACAGCCCACCCGAATCCCCGGTACCGCGAAGCAGCCGATTTCATTCTCTCCGAGGCCGAGTCCCTCTCCCTCCAGTCCCAGACTCTCGAGTTCGTCCCCGGCAAACCGCTCGTCCTCCTCAAATGGCCAGGCTCCGACCCTTCTCTCCCCTCCGTTCTCCTCAACTCCCACACCGACGTCGTCCCGGCCGAGCACGACAAGTGGGCCCACCCACCTTTCTCGGCCAACTTGGATCCGAACGGCAACGTTTACGCCAGGGGATCCCAGGACATGAAGTGCGTTGGGATTCAGTACCTGGAGGCCATTCGGCGGCTCAAGGCTTCTGGGTTTAAGCCCAAAAGGTCTGTTTACTTGTCGTTTGTGCCTGATGAGGAGATTGGGGGCCACGACGGCGCTGAGAAGCTGGCTGAATCTGATGTTTTCAAGGGATTGAACGTAGGAATTGTGCTTGATGAAGAGATTTTAACGTCTGGGTTGGGGTTGGCCTCGCCGACTGAGAATTACAGGGCGTTCTATGCAGAGAGGTGTCCCATGTGGCTGGTGATCAAGGCTACTGGGGCTCCTGGTCATGGGGCTAAGCTCTATGACAACACTGCCATGGAGAATCTCTTGAAAAGCATTGAGAGTGTGAGGCGGTTCCGGGCTTCACAGTTTGATTTGGTGAAGGCAGGTTTGAAGGCTGAAGGCGAGGTCGTCTCTGTTAATATGGTGTTTTTGAAAGCTGGCACTCCGACTCCAACAGGTTTTGTCATGAATTTGCAGCCATCTGAAGCAGAAGCAGGCCTTGATATTAGGGTCCCACCAACTGCTGATCAGGAGTCTTTGGAGAAACGGATAGCTGAAGAATGGGCCCCTGCTTCACGAAACATGACATTCAGGTTCAAGCATAAGGTTTCTGTGCTTGATAAGGCGGGGAGGCCAATCCTTACAGCAACTGATAGTTCAAACCCCTGGTGGGCTCTTCTTGAAGATGCTGTCAAAAAAGCTAATGGGAAACTTGGTAAGCCAGAGATTTTTCCTGCTTCAACAGATGCTCGCTATTTCCGGAATCTGGGCTTGCCAGCGATTGGATTCTCTCCGATGGCTAACACTCCTATTCTTCTTCATGACCATAATGAGTTTCTAAACAAAGACGAATACTTGAAAGGAATTGATATTTACGAATCTGTTATCAAGGCCTATGCGTCTTACGTTGATGGTGGGATAAGCGCGGTTCCTAGAGACGAGCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004046 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005615 GO:0016787 GO:0016810 GO:0016811 GO:0031982 GO:0042802 GO:0043226 GO:0043227 GO:0043230 GO:0044421 GO:0070062 GO:1903561

KEGG Pathways

Metabolic & Signaling

ko00220 ko01100 ko01110 ko01130 ko01210 ko01230 map00220 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

450

Amino Acids

49.52

Weight (kDa)

5.93

Isoelectric Point (pI)

36.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	92 - 431	1.5e-36	Peptidase family M20/M25/M40

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004417)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38220 AT4G38220
fragaria_vesca	FvH4_2g16990
malus_domestica	MD05G1143000.v1.1 MD10G1142800.v1.1 MD15G1413100.v1.1
prunus_persica	Prupe.8G181800_v2.0.a1 Prupe.8G181800_v2.0.a1
pyrus_communis	pycom05g13750 pycom10g12270
rosa_chinensis	RchiOBHm_Chr2g0126171 RchiOBHm_Chr6g0281181
rosa_laevigata	RLG00000012987
rosa_multiflora	Rmu_co8196198.1_g000001 Rmu_co8363365.1_g000001 Rmu_sc0001705.1_g000022 Rmu_sc0012209.1_g000001 Rmu_sc0026953.1_g000001 Rmu_sc0034918.1_g000001 Rmu_sc0035650.1_g000001
rosa_rugosa	Rorug06G0139500 Rorug06G0139500
rosa_samantha	Rh2BG327100 Rh6AG251300 Rh6BG182200 Rh6BG254500 Rh6CG253900 Rh6DG246000
rosa_wichuraiana	Rw2G031780 Rw3G017710 Rw6G021830 Rw7G031480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	297
Acc36I	ACCTGC	1 cut(s)	776
Acc65I	GGTACC	1 cut(s)	138
AccB1I	GGYRCC	1 cut(s)	138
AccB7I	CCANNNNNTGG	1 cut(s)	1184
AccBSI	CCGCTC	1 cut(s)	226
AccII	CGCG	2 cut(s)	144, 1328
AccIII	TCCGGA	1 cut(s)	1161
AciI	CCGC	6 cut(s)	142, 224, 427, 751, 1028, 1328
AclI	AACGTT	1 cut(s)	363
AclWI	GGATC	4 cut(s)	344, 357, 372, 385
AcoI	YGGCCR	4 cut(s)	60, 242, 303, 339
AcsI	RAATTY	1 cut(s)	871
AcuI	CTGAAG	3 cut(s)	819, 906, 978
AcyI	GRCGYC	1 cut(s)	294
AfaI	GTAC	2 cut(s)	140, 410
AfiI	CCNNNNNNNGG	5 cut(s)	426, 686, 1072, 1168, 1184
AjnI	CCWGG	6 cut(s)	244, 371, 381, 411, 679, 1067
AluBI	AGCT	7 cut(s)	7, 520, 697, 839, 956, 1103, 1345
AluI	AGCT	7 cut(s)	7, 520, 697, 839, 956, 1103, 1345
Alw21I	GWGCWC	1 cut(s)	312
Alw26I	GTCTC	3 cut(s)	23, 817, 1332
AlwI	GGATC	4 cut(s)	344, 357, 372, 385
AlwNI	CAGNNNCTG	2 cut(s)	412, 1052
Ama87I	CYCGRG	1 cut(s)	203
Aor13HI	TCCGGA	1 cut(s)	1161
ApaI	GGGCCC	2 cut(s)	325, 970
ApeKI	GCWGC	2 cut(s)	149, 877
ApoI	RAATTY	1 cut(s)	871
ArsI	GACNNNNNNTTYG	2 cut(s)	213, 245
Asp718I	GGTACC	1 cut(s)	138
AspLEI	GCGC	2 cut(s)	512, 1328
AspS9I	GGNCC	6 cut(s)	321, 322, 499, 913, 966, 967
AsuC2I	CCSGG	4 cut(s)	136, 216, 302, 758
AsuHPI	GGTGA	2 cut(s)	670, 790
AvaI	CYCGRG	1 cut(s)	203
AvaII	GGWCC	1 cut(s)	913
BaeGI	GKGCMC	2 cut(s)	325, 970
BalI	TGGCCA	1 cut(s)	244
BamHI	GGATCC	2 cut(s)	349, 377
BanI	GGYRCC	1 cut(s)	138
BanII	GRGCYC	4 cut(s)	325, 679, 970, 1078
BbsI	GAAGAC	1 cut(s)	5
Bbv12I	GWGCWC	1 cut(s)	312
BbvI	GCAGC	2 cut(s)	161, 889
BccI	CCATC	3 cut(s)	889, 1192, 1307
BceAI	ACGGC	3 cut(s)	75, 373, 523
BcgI	CGANNNNNNTGC	2 cut(s)	208, 242
BciT130I	CCWGG	6 cut(s)	246, 373, 383, 413, 681, 1069
BclI	TGATCA	2 cut(s)	660, 928
BcnI	CCSGG	4 cut(s)	136, 216, 302, 758
BcoDI	GTCTC	3 cut(s)	23, 817, 1332
BfaI	CTAG	1 cut(s)	1335
BfoI	RGCGCY	1 cut(s)	513
BfuAI	ACCTGC	1 cut(s)	776
BglI	GCCNNNNNGGC	1 cut(s)	507
BisI	GCNGC	3 cut(s)	150, 428, 878
BlpI	GCTNAGC	1 cut(s)	693
BlsI	GCNGC	3 cut(s)	151, 429, 879
Bme18I	GGWCC	1 cut(s)	913
BmeT110I	CYCGRG	1 cut(s)	203
BmgT120I	GGNCC	6 cut(s)	321, 322, 499, 913, 966, 967
BmrI	ACTGGG	1 cut(s)	681
BmsI	GCATC	2 cut(s)	1078, 1138
BmuI	ACTGGG	1 cut(s)	681
BpiI	GAAGAC	1 cut(s)	5
BplI	GAGNNNNNCTC	4 cut(s)	167, 199, 797, 829
BpmI	CTGGAG	2 cut(s)	173, 434
Bpu1102I	GCTNAGC	1 cut(s)	693
BpuEI	CTTGAG	1 cut(s)	416
BpuMI	CCSGG	4 cut(s)	136, 216, 302, 758
BsaHI	GRCGYC	1 cut(s)	294
BsaJI	CCNNGG	7 cut(s)	134, 168, 214, 372, 381, 714, 1067
BsaWI	WCCGGW	1 cut(s)	1161
BsaXI	ACNNNNNCTCC	6 cut(s)	90, 120, 661, 691, 835, 865
Bsc4I	CCNNNNNNNGG	5 cut(s)	426, 686, 1072, 1168, 1184
Bse1I	ACTGG	2 cut(s)	190, 676
BseAI	TCCGGA	1 cut(s)	1161
BseBI	CCWGG	6 cut(s)	246, 373, 383, 413, 681, 1069
BseDI	CCNNGG	7 cut(s)	134, 168, 214, 372, 381, 714, 1067
BseLI	CCNNNNNNNGG	5 cut(s)	426, 686, 1072, 1168, 1184
BseMII	CTCAG	2 cut(s)	504, 603
BseNI	ACTGG	2 cut(s)	190, 676
BseRI	GAGGAG	3 cut(s)	224, 266, 503
BseSI	GKGCMC	2 cut(s)	325, 970
BseX3I	CGGCCG	1 cut(s)	303
BseXI	GCAGC	2 cut(s)	161, 889
Bsh1236I	CGCG	2 cut(s)	144, 1328
Bsh1285I	CGRYCG	1 cut(s)	306
BshNI	GGYRCC	1 cut(s)	138
BsiEI	CGRYCG	1 cut(s)	306
BsiHKAI	GWGCWC	1 cut(s)	312
BsiHKCI	CYCGRG	1 cut(s)	203
BsiSI	CCGG	5 cut(s)	136, 216, 302, 757, 1162
BslFI	GGGAC	6 cut(s)	163, 178, 197, 284, 630, 899
BslI	CCNNNNNNNGG	5 cut(s)	426, 686, 1072, 1168, 1184
BsmAI	GTCTC	3 cut(s)	23, 817, 1332
BsmBI	CGTCTC	3 cut(s)	23, 817, 1332
BsmFI	GGGAC	6 cut(s)	163, 178, 197, 284, 630, 899
BsoBI	CYCGRG	1 cut(s)	203
Bsp120I	GGGCCC	2 cut(s)	321, 966
Bsp1286I	GDGCHC	5 cut(s)	312, 325, 679, 970, 1078
Bsp13I	TCCGGA	1 cut(s)	1161
Bsp143I	GATC	4 cut(s)	349, 377, 660, 928
Bsp1720I	GCTNAGC	1 cut(s)	693
Bsp19I	CCATGG	1 cut(s)	714
BspACI	CCGC	6 cut(s)	142, 224, 427, 751, 1028, 1328
BspCNI	CTCAG	2 cut(s)	505, 604
BspEI	TCCGGA	1 cut(s)	1161
BspFNI	CGCG	2 cut(s)	144, 1328
BspHI	TCATGA	2 cut(s)	867, 1222
BspMI	ACCTGC	1 cut(s)	776
BspPI	GGATC	4 cut(s)	344, 357, 372, 385
BspQI	GCTCTTC	1 cut(s)	1083
BspT107I	GGYRCC	1 cut(s)	138
BsrBI	CCGCTC	1 cut(s)	226
BsrI	ACTGG	2 cut(s)	190, 676
BssECI	CCNNGG	7 cut(s)	134, 168, 214, 372, 381, 714, 1067
BssMI	GATC	4 cut(s)	349, 377, 660, 928
BssNI	GRCGYC	1 cut(s)	294
BssT1I	CCWWGG	1 cut(s)	714
Bst2UI	CCWGG	6 cut(s)	246, 373, 383, 413, 681, 1069
Bst4CI	ACNGT	2 cut(s)	93, 768
Bst6I	CTCTTC	2 cut(s)	567, 1083
BstACI	GRCGYC	1 cut(s)	294
BstC8I	GCNNGC	6 cut(s)	522, 841, 898, 1153, 1175, 1179
BstDEI	CTNAG	3 cut(s)	513, 612, 693
BstDSI	CCRYGG	1 cut(s)	714
BstFNI	CGCG	2 cut(s)	144, 1328
BstH2I	RGCGCY	1 cut(s)	513
BstHHI	GCGC	2 cut(s)	512, 1328
BstKTI	GATC	4 cut(s)	352, 380, 663, 931
BstMAI	GTCTC	3 cut(s)	23, 817, 1332
BstMBI	GATC	4 cut(s)	349, 377, 660, 928
BstMCI	CGRYCG	1 cut(s)	306
BstMWI	GCNNNNNNNGC	3 cut(s)	507, 632, 674
BstNI	CCWGG	6 cut(s)	246, 373, 383, 413, 681, 1069
BstSLI	GKGCMC	2 cut(s)	325, 970
BstUI	CGCG	2 cut(s)	144, 1328
BstV1I	GCAGC	2 cut(s)	161, 889
BstV2I	GAAGAC	1 cut(s)	5
BstX2I	RGATCY	2 cut(s)	349, 377
BstXI	CCANNNNNNTGG	1 cut(s)	655
BstYI	RGATCY	2 cut(s)	349, 377
BstZI	CGGCCG	1 cut(s)	303
BtgI	CCRYGG	1 cut(s)	714
BtsI	GCAGTG	1 cut(s)	708
BtsIMutI	CAGTG	1 cut(s)	708
BveI	ACCTGC	1 cut(s)	776
Cac8I	GCNNGC	6 cut(s)	522, 841, 898, 1153, 1175, 1179
CaiI	CAGNNNCTG	2 cut(s)	412, 1052
CciI	TCATGA	2 cut(s)	867, 1222
CfoI	GCGC	2 cut(s)	512, 1328
Cfr13I	GGNCC	6 cut(s)	321, 322, 499, 913, 966, 967
CseI	GACGC	1 cut(s)	1290
Csp6I	GTAC	2 cut(s)	139, 409
CviAII	CATG	7 cut(s)	388, 649, 686, 715, 868, 985, 1223
CviQI	GTAC	2 cut(s)	139, 409
DdeI	CTNAG	3 cut(s)	513, 612, 693
DpnI	GATC	4 cut(s)	351, 379, 662, 930
DpnII	GATC	4 cut(s)	349, 377, 660, 928
EaeI	YGGCCR	4 cut(s)	60, 242, 303, 339
EagI	CGGCCG	1 cut(s)	303
Eam1104I	CTCTTC	2 cut(s)	567, 1083
EarI	CTCTTC	2 cut(s)	567, 1083
EclXI	CGGCCG	1 cut(s)	303
Eco130I	CCWWGG	1 cut(s)	714
Eco147I	AGGCCT	2 cut(s)	900, 1295
Eco24I	GRGCYC	4 cut(s)	325, 679, 970, 1078
Eco47I	GGWCC	1 cut(s)	913
Eco52I	CGGCCG	1 cut(s)	303
Eco57I	CTGAAG	3 cut(s)	819, 906, 978
Eco88I	CYCGRG	1 cut(s)	203
EcoO109I	RGGNCCY	2 cut(s)	913, 967
EcoRII	CCWGG	6 cut(s)	244, 371, 381, 411, 679, 1067
EcoT14I	CCWWGG	1 cut(s)	714
EcoT38I	GRGCYC	4 cut(s)	325, 679, 970, 1078
ErhI	CCWWGG	1 cut(s)	714
Esp3I	CGTCTC	3 cut(s)	23, 817, 1332
FaeI	CATG	7 cut(s)	391, 652, 689, 718, 871, 988, 1226
FalI	AAGNNNNNCTT	2 cut(s)	885, 917
FaqI	GGGAC	6 cut(s)	163, 178, 197, 284, 630, 899
FatI	CATG	7 cut(s)	387, 648, 685, 714, 867, 984, 1222
FauI	CCCGC	1 cut(s)	1021
FbaI	TGATCA	2 cut(s)	660, 928
Fnu4HI	GCNGC	3 cut(s)	150, 428, 878
FriOI	GRGCYC	4 cut(s)	325, 679, 970, 1078
Fsp4HI	GCNGC	3 cut(s)	150, 428, 878
FspBI	CTAG	1 cut(s)	1335
GlaI	GCGC	2 cut(s)	511, 1327
GluI	GCNGC	3 cut(s)	150, 428, 878
GsuI	CTGGAG	2 cut(s)	173, 434
HaeII	RGCGCY	1 cut(s)	513
HapII	CCGG	5 cut(s)	136, 216, 302, 757, 1162
HgaI	GACGC	1 cut(s)	1290
HhaI	GCGC	2 cut(s)	512, 1328
Hin1I	GRCGYC	1 cut(s)	294
Hin1II	CATG	7 cut(s)	391, 652, 689, 718, 871, 988, 1226
Hin6I	GCGC	2 cut(s)	510, 1326
HinP1I	GCGC	2 cut(s)	510, 1326
HpaII	CCGG	5 cut(s)	136, 216, 302, 757, 1162
HphI	GGTGA	2 cut(s)	670, 790
Hpy166II	GTNNAC	2 cut(s)	367, 466
Hpy188I	TCNGA	8 cut(s)	73, 169, 253, 354, 532, 849, 886, 1197
Hpy188III	TCNNGA	8 cut(s)	203, 727, 868, 932, 978, 1082, 1162, 1223
Hpy8I	GTNNAC	2 cut(s)	367, 466
Hpy99I	CGWCG	4 cut(s)	77, 296, 299, 509
HpyAV	CCTTC	4 cut(s)	267, 775, 787, 794
HpyCH4III	ACNGT	2 cut(s)	93, 768
HpyCH4IV	ACGT	5 cut(s)	294, 363, 552, 584, 1308
HpyCH4V	TGCA	3 cut(s)	24, 635, 877
HpyF10VI	GCNNNNNNNGC	3 cut(s)	507, 632, 674
HpyF3I	CTNAG	3 cut(s)	513, 612, 693
HpySE526I	ACGT	5 cut(s)	294, 363, 552, 584, 1308
Hsp92I	GRCGYC	1 cut(s)	294
Hsp92II	CATG	7 cut(s)	391, 652, 689, 718, 871, 988, 1226
HspAI	GCGC	2 cut(s)	510, 1326
KflI	GGGWCCC	1 cut(s)	913
Kpn2I	TCCGGA	1 cut(s)	1161
KpnI	GGTACC	1 cut(s)	142
Ksp22I	TGATCA	2 cut(s)	660, 928
Kzo9I	GATC	4 cut(s)	349, 377, 660, 928
LguI	GCTCTTC	1 cut(s)	1083
LmnI	GCTCC	3 cut(s)	4, 254, 682
Lsp1109I	GCAGC	2 cut(s)	161, 889
LweI	GCATC	2 cut(s)	1078, 1138
MaeI	CTAG	1 cut(s)	1335
MaeII	ACGT	5 cut(s)	294, 363, 552, 584, 1308
MalI	GATC	4 cut(s)	351, 379, 662, 930
MbiI	CCGCTC	1 cut(s)	226
MboI	GATC	4 cut(s)	349, 377, 660, 928
MboII	GAAGA	7 cut(s)	5, 584, 971, 1070, 1097, 1208, 1211
MflI	RGATCY	2 cut(s)	349, 377
MhlI	GDGCHC	5 cut(s)	312, 325, 679, 970, 1078
MlsI	TGGCCA	1 cut(s)	244
MluCI	AATT	4 cut(s)	558, 616, 871, 1266
MluNI	TGGCCA	1 cut(s)	244
MlyI	GAGTC	4 cut(s)	185, 193, 844, 944
MmeI	TCCRAC	4 cut(s)	96, 276, 872, 878
Mox20I	TGGCCA	1 cut(s)	244
MroI	TCCGGA	1 cut(s)	1161
MscI	TGGCCA	1 cut(s)	244
MseI	TTAA	3 cut(s)	447, 581, 819
MslI	CAYNNNNRTG	1 cut(s)	713
Msp20I	TGGCCA	1 cut(s)	244
MspI	CCGG	5 cut(s)	136, 216, 302, 757, 1162
MvaI	CCWGG	6 cut(s)	246, 373, 383, 413, 681, 1069
MvnI	CGCG	2 cut(s)	144, 1328
MwoI	GCNNNNNNNGC	3 cut(s)	507, 632, 674
NciI	CCSGG	4 cut(s)	136, 216, 302, 758
NcoI	CCATGG	1 cut(s)	714
NdeII	GATC	4 cut(s)	349, 377, 660, 928
NlaIII	CATG	7 cut(s)	391, 652, 689, 718, 871, 988, 1226
NmeAIII	GCCGAG	3 cut(s)	199, 317, 331
PaeR7I	CTCGAG	1 cut(s)	203
PagI	TCATGA	2 cut(s)	867, 1222
PceI	AGGCCT	2 cut(s)	900, 1295
PciSI	GCTCTTC	1 cut(s)	1083
PcsI	WCGNNNNNNNCGW	2 cut(s)	97, 303
PfeI	GAWTC	7 cut(s)	130, 403, 527, 721, 1165, 1188, 1280
PflFI	GACNNNGTC	1 cut(s)	296
PflMI	CCANNNNNTGG	1 cut(s)	1184
PkrI	GCNGC	3 cut(s)	151, 429, 879
PleI	GAGTC	4 cut(s)	184, 193, 844, 943
PpsI	GAGTC	4 cut(s)	184, 193, 844, 943
PpuMI	RGGWCCY	1 cut(s)	913
Psp1406I	AACGTT	1 cut(s)	363
Psp5II	RGGWCCY	1 cut(s)	913
Psp6I	CCWGG	6 cut(s)	244, 371, 381, 411, 679, 1067
PspGI	CCWGG	6 cut(s)	244, 371, 381, 411, 679, 1067
PspOMI	GGGCCC	2 cut(s)	321, 966
PspPI	GGNCC	6 cut(s)	321, 322, 499, 913, 966, 967
PspPPI	RGGWCCY	1 cut(s)	913
PstNI	CAGNNNCTG	2 cut(s)	412, 1052
PsuI	RGATCY	2 cut(s)	349, 377
PsyI	GACNNNGTC	1 cut(s)	296
RsaI	GTAC	2 cut(s)	140, 410
RsaNI	GTAC	2 cut(s)	139, 409
RseI	CAYNNNNRTG	1 cut(s)	713
SapI	GCTCTTC	1 cut(s)	1083
SaqAI	TTAA	3 cut(s)	447, 581, 819
SatI	GCNGC	3 cut(s)	150, 428, 878
Sau3AI	GATC	4 cut(s)	349, 377, 660, 928
Sau96I	GGNCC	6 cut(s)	321, 322, 499, 913, 966, 967
SchI	GAGTC	4 cut(s)	185, 193, 844, 944
SduI	GDGCHC	5 cut(s)	312, 325, 679, 970, 1078
SfaNI	GCATC	2 cut(s)	1078, 1138
Sfr274I	CTCGAG	1 cut(s)	203
SinI	GGWCC	1 cut(s)	913
SlaI	CTCGAG	1 cut(s)	203
SmiMI	CAYNNNNRTG	1 cut(s)	713
SmlI	CTYRAG	2 cut(s)	203, 431
SmoI	CTYRAG	2 cut(s)	203, 431
Sse9I	AATT	4 cut(s)	558, 616, 871, 1266
SseBI	AGGCCT	2 cut(s)	900, 1295
SsiI	CCGC	6 cut(s)	142, 224, 427, 751, 1028, 1328
SspMI	CTAG	1 cut(s)	1335
StuI	AGGCCT	2 cut(s)	900, 1295
StyI	CCWWGG	1 cut(s)	714
TaaI	ACNGT	2 cut(s)	93, 768
TaiI	ACGT	5 cut(s)	297, 366, 555, 587, 1311
TaqI	TCGA	1 cut(s)	204
TasI	AATT	4 cut(s)	558, 616, 871, 1266
TauI	GCSGC	1 cut(s)	430
TfiI	GAWTC	7 cut(s)	130, 403, 527, 721, 1165, 1188, 1280
Tru1I	TTAA	3 cut(s)	447, 581, 819
Tru9I	TTAA	3 cut(s)	447, 581, 819
TscAI	CASTG	1 cut(s)	715
TseI	GCWGC	2 cut(s)	149, 877
TspDTI	ATGAA	5 cut(s)	148, 404, 585, 884, 1211
TspGWI	ACGGA	2 cut(s)	259, 964
TspRI	CASTG	1 cut(s)	715
Tth111I	GACNNNGTC	1 cut(s)	296
Van91I	CCANNNNNTGG	1 cut(s)	1184
VpaK11BI	GGWCC	1 cut(s)	913
XapI	RAATTY	1 cut(s)	871
XhoI	CTCGAG	1 cut(s)	203
XspI	CTAG	1 cut(s)	1335
ZraI	GACGTC	1 cut(s)	295

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.