MD15G1413100.v1.1

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004417
Physical Location & Seq
Reverse (-)
51268602 .. 51269096
495 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1413100.v1.1.491

Sequence Viewer

Length: 228 bp
ATGAAGTGCGTCGGGATTCAGTACTTGGAGGCGATTCGACGGCTCAAGGCTTCTGGGTTTAAGCCCAAAAGGTCTGTTTATTTGTCATATGGTCCTGATGAGGAGATTGGGGGACACGACGGTGCTGAGAAGCTGGCTGAATCTGATGTTTTCAAGGGATTGAACGTCGGAATTGTGCTTGATGAAGCCATATTGTCAAATTTGTGCACAGAACTTGTCTGGTTTTGA

Protein Analysis

76

Amino Acids

8.29

Weight (kDa)

5.26

Isoelectric Point (pI)

27.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 1 - 59 1e-08 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 199
AfaI GTAC 1 cut(s) 23
AgsI TTSAA 2 cut(s) 154, 163
AleI CACNNNNGTG 1 cut(s) 120
AluBI AGCT 1 cut(s) 133
AluI AGCT 1 cut(s) 133
Alw21I GWGCWC 1 cut(s) 209
Alw44I GTGCAC 1 cut(s) 205
ApaLI GTGCAC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 92
AvaII GGWCC 1 cut(s) 92
BaeGI GKGCMC 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 56
BmcAI AGTACT 1 cut(s) 23
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 29
BseMII CTCAG 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 209
BsiHKAI GWGCWC 1 cut(s) 209
BslFI GGGAC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 209
BspCNI CTCAG 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 1 cut(s) 126
BstSLI GKGCMC 1 cut(s) 209
Cac8I GCNNGC 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 22
CviJI RGCY 6 cut(s) 43, 50, 64, 133, 137, 188
CviKI_1 RGCY 6 cut(s) 43, 50, 64, 133, 137, 188
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 92
FaiI YATR 3 cut(s) 88, 90, 191
FaqI GGGAC 1 cut(s) 126
FauNDI CATATG 1 cut(s) 88
HinfI GANTC 3 cut(s) 16, 34, 140
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 2 cut(s) 145, 170
Hpy188III TCNNGA 2 cut(s) 13, 95
Hpy8I GTNNAC 1 cut(s) 207
Hpy99I CGWCG 4 cut(s) 14, 42, 122, 170
HpyCH4III ACNGT 1 cut(s) 122
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 1 cut(s) 207
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 165
LpnPI CCDG 4 cut(s) 39, 108, 119, 205
MaeII ACGT 1 cut(s) 165
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 2 cut(s) 171, 199
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 2 cut(s) 22, 94
MseI TTAA 1 cut(s) 60
MslI CAYNNNNRTG 1 cut(s) 120
NdeI CATATG 1 cut(s) 88
OliI CACNNNNGTG 1 cut(s) 120
PfeI GAWTC 3 cut(s) 16, 34, 140
PspPI GGNCC 1 cut(s) 92
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 1 cut(s) 60
Sau96I GGNCC 1 cut(s) 92
ScaI AGTACT 1 cut(s) 23
SduI GDGCHC 1 cut(s) 209
SetI ASST 3 cut(s) 74, 135, 168
SgeI CNNG 9 cut(s) 25, 37, 58, 66, 107, 128, 146, 166, 191
SinI GGWCC 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 2 cut(s) 171, 199
TaaI ACNGT 1 cut(s) 122
TaiI ACGT 1 cut(s) 168
TaqI TCGA 1 cut(s) 37
TasI AATT 2 cut(s) 171, 199
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 3 cut(s) 16, 34, 140
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TspDTI ATGAA 2 cut(s) 17, 198
VneI GTGCAC 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 1 cut(s) 199
ZrmI AGTACT 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.