FvH4_2g21590

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005523
Physical Location & Seq
Reverse (-)
17937079 .. 17938014
936 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21590.t1

Sequence Viewer

Length: 453 bp
ATGGAAGGAGACTCGAGCAGAGGCAGCAAGGCAAAACAAGAGGGAAAGAAATGCGTCAAGTACAGAGGAGTGAGGAGAAGGCCATGGGGGAAGTTCGCAGCAGAAATACGAGATTCAACGAGGCATGGAGAAGGAGCTCGGTTGTGGCTCGGGACTTTCAACACTGCAGAAGAGGCTGCAAGGGCTTATGACAGTGCTGCTTTTTCAATGAGGGGTGCTACTGCAATTCTCAACTTCCCAAGTGAGCATGGTTTGCCAGATAGTTCTGCTGCTTCTGCTGTTTCCTCACCATCTTCTTCTTCATCATCCCCAACATCCTTTTCGGCATCATATTCAACATTGCAGAATGTGGAAGAGACTACAAGGACAGAACCCAGAAGAGAAGTTTTCGAGATCGAGTGTTTAGATGACAAGGTTTTAGAGGACCTCCTTGATTTTGACAGGAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

151

Amino Acids

16.46

Weight (kDa)

6.16

Isoelectric Point (pI)

67.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 20 - 72 8.8e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 62
AgsI TTSAA 4 cut(s) 117, 160, 207, 336
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 2 cut(s) 137, 450
AluI AGCT 2 cut(s) 137, 450
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 2 cut(s) 3, 350
Ama87I CYCGRG 2 cut(s) 13, 149
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 5 cut(s) 24, 98, 176, 197, 269
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 279
AvaI CYCGRG 2 cut(s) 13, 149
AvaII GGWCC 1 cut(s) 424
BanII GRGCYC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 5 cut(s) 36, 110, 163, 184, 256
BccI CCATC 1 cut(s) 298
BcoDI GTCTC 2 cut(s) 3, 350
BfaI CTAG 1 cut(s) 451
BfmI CTRYAG 1 cut(s) 165
BisI GCNGC 5 cut(s) 25, 99, 177, 198, 270
BlsI GCNGC 5 cut(s) 26, 100, 178, 199, 271
Bme18I GGWCC 1 cut(s) 424
BmeT110I CYCGRG 2 cut(s) 13, 149
BmgT120I GGNCC 1 cut(s) 424
BmsI GCATC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 83
Bse3DI GCAATG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 83
BseGI GGATG 2 cut(s) 305, 314
BseMI GCAATG 1 cut(s) 338
BseRI GAGGAG 2 cut(s) 81, 88
BseXI GCAGC 5 cut(s) 36, 110, 163, 184, 256
BshFI GGCC 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 139
BsiHKCI CYCGRG 2 cut(s) 13, 149
BslFI GGGAC 1 cut(s) 166
BsmAI GTCTC 2 cut(s) 3, 350
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 1 cut(s) 82
BsoBI CYCGRG 2 cut(s) 13, 149
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 1 cut(s) 393
Bsp19I CCATGG 1 cut(s) 83
BspANI GGCC 1 cut(s) 82
BspMAI CTGCAG 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 338
BssECI CCNNGG 1 cut(s) 83
BssMI GATC 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 3 cut(s) 165, 348, 373
BstAPI GCANNNNNTGC 1 cut(s) 253
BstDSI CCRYGG 1 cut(s) 83
BstF5I GGATG 2 cut(s) 305, 314
BstKTI GATC 1 cut(s) 396
BstMAI GTCTC 2 cut(s) 3, 350
BstMBI GATC 1 cut(s) 393
BstMWI GCNNNNNNNGC 5 cut(s) 24, 173, 182, 253, 275
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 5 cut(s) 36, 110, 163, 184, 256
BsuRI GGCC 1 cut(s) 82
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 2 cut(s) 305, 314
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 162, 199
Cfr13I GGNCC 1 cut(s) 424
CseI GACGC 1 cut(s) 43
Csp6I GTAC 1 cut(s) 61
CviAII CATG 3 cut(s) 84, 125, 248
CviJI RGCY 6 cut(s) 82, 137, 148, 176, 185, 450
CviKI_1 RGCY 6 cut(s) 82, 137, 148, 176, 185, 450
CviQI GTAC 1 cut(s) 61
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
Eam1104I CTCTTC 3 cut(s) 165, 348, 373
EarI CTCTTC 3 cut(s) 165, 348, 373
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 137
Eco88I CYCGRG 2 cut(s) 13, 149
EcoICRI GAGCTC 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 83
FaeI CATG 3 cut(s) 87, 128, 251
FaiI YATR 5 cut(s) 85, 126, 189, 249, 331
FaqI GGGAC 1 cut(s) 166
FatI CATG 3 cut(s) 83, 124, 247
Fnu4HI GCNGC 5 cut(s) 25, 99, 177, 198, 270
FokI GGATG 2 cut(s) 292, 301
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 5 cut(s) 25, 99, 177, 198, 270
FspBI CTAG 1 cut(s) 451
GluI GCNGC 5 cut(s) 25, 99, 177, 198, 270
HaeIII GGCC 1 cut(s) 82
HgaI GACGC 1 cut(s) 43
Hin1II CATG 3 cut(s) 87, 128, 251
HinfI GANTC 2 cut(s) 11, 113
HphI GGTGA 1 cut(s) 279
Hpy188III TCNNGA 2 cut(s) 151, 391
HpyAV CCTTC 2 cut(s) 72, 125
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4V TGCA 4 cut(s) 167, 179, 224, 343
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 173, 182, 253, 275
Hsp92II CATG 3 cut(s) 87, 128, 251
Kzo9I GATC 1 cut(s) 393
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 3 cut(s) 270, 388, 427
Lsp1109I GCAGC 5 cut(s) 36, 110, 163, 184, 256
LweI GCATC 1 cut(s) 335
MaeI CTAG 1 cut(s) 451
MalI GATC 1 cut(s) 395
MboI GATC 1 cut(s) 393
MboII GAAGA 6 cut(s) 182, 285, 288, 291, 365, 390
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 1 cut(s) 225
MlyI GAGTC 1 cut(s) 5
MwoI GCNNNNNNNGC 5 cut(s) 24, 173, 182, 253, 275
NcoI CCATGG 1 cut(s) 83
NdeII GATC 1 cut(s) 393
NlaIII CATG 3 cut(s) 87, 128, 251
PaeR7I CTCGAG 1 cut(s) 13
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 5 cut(s) 26, 100, 178, 199, 271
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
PpuMI RGGWCCY 1 cut(s) 424
Psp124BI GAGCTC 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 424
PspPI GGNCC 1 cut(s) 424
PspPPI RGGWCCY 1 cut(s) 424
PspXI VCTCGAGB 1 cut(s) 13
PstI CTGCAG 1 cut(s) 169
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SacI GAGCTC 1 cut(s) 139
SatI GCNGC 5 cut(s) 25, 99, 177, 198, 270
Sau3AI GATC 1 cut(s) 393
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 1 cut(s) 5
SduI GDGCHC 1 cut(s) 139
SetI ASST 4 cut(s) 139, 417, 429, 452
SfaNI GCATC 1 cut(s) 335
SfcI CTRYAG 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 13
SinI GGWCC 1 cut(s) 424
SlaI CTCGAG 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
Sse9I AATT 1 cut(s) 225
SspMI CTAG 1 cut(s) 451
SstI GAGCTC 1 cut(s) 139
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 1 cut(s) 194
TaqI TCGA 3 cut(s) 14, 390, 396
TasI AATT 1 cut(s) 225
TatI WGTACW 1 cut(s) 60
TfiI GAWTC 1 cut(s) 113
TscAI CASTG 2 cut(s) 169, 199
TseI GCWGC 5 cut(s) 24, 98, 176, 197, 269
TspDTI ATGAA 1 cut(s) 291
TspRI CASTG 2 cut(s) 169, 199
VpaK11BI GGWCC 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 13
XspI CTAG 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.