RLG00000012419

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005523
Physical Location & Seq
Forward (+)
18610698 .. 18611150
453 bp
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UTR
Exon/CDS
Intron
RLM00000012419

Sequence Viewer

Length: 453 bp
ATGGAAGGAGACTCAAGTAGAGGCAGCAAGGCAAAACAAGTGGGACAGAAAGATGTCAAGTACAGAGGAGTGAGGAGAAGGCCATGGGGGAAGTTCGCAGCAGAAATACGCGATTCAACGAGGCATGGAGAAGGAGCCCGGTTGTGGCTGGGGACTTTCAACACTGCAGAAGAGGCTGCAAGGGCTTATGATAGTGCTGCTTTTTCGATGAGGGGTGCTCTTGCAATTCTCAACTTCCCACGTGAGCATGGTTTGCCAGATAGTTCTGCTGCTTCTGCTGTTTCCTCATCATCTTCATCTTCGTCATCCCCAGCATCCATTTCAGCATCATCTTCAACATTGCAGAATGTGGAAGGCACAACTAGGACAGAACCCAGAAGAGAAGTCTTCGAGATTGAGTGTTTAGATGACAAGGTTTTAGAGGACCTTCTTGATTTTAACAGGAAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

151

Amino Acids

16.3

Weight (kDa)

7.89

Isoelectric Point (pI)

62.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 20 - 72 8.8e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 111
AcvI CACGTG 1 cut(s) 242
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 1 cut(s) 144
AgsI TTSAA 3 cut(s) 117, 160, 336
AluBI AGCT 1 cut(s) 450
AluI AGCT 1 cut(s) 450
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 1 cut(s) 3
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 5 cut(s) 24, 98, 176, 197, 269
AspS9I GGNCC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 139
AvaII GGWCC 1 cut(s) 424
BanII GRGCYC 1 cut(s) 139
BbrPI CACGTG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 5 cut(s) 36, 110, 163, 184, 256
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 1 cut(s) 3
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 165
BisI GCNGC 5 cut(s) 25, 99, 177, 198, 270
BlsI GCNGC 5 cut(s) 26, 100, 178, 199, 271
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 2 cut(s) 323, 335
BpiI GAAGAC 1 cut(s) 379
BplI GAGNNNNNCTC 2 cut(s) 202, 234
BpuMI CCSGG 1 cut(s) 139
BsaAI YACGTR 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 83
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 83
BseGI GGATG 2 cut(s) 305, 314
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMI GCAATG 1 cut(s) 338
BseRI GAGGAG 2 cut(s) 81, 88
BseXI GCAGC 5 cut(s) 36, 110, 163, 184, 256
BseYI CCCAGC 2 cut(s) 148, 310
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 220
BsiSI CCGG 1 cut(s) 139
BslFI GGGAC 2 cut(s) 57, 166
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 3
BsmFI GGGAC 2 cut(s) 57, 166
BsnI GGCC 1 cut(s) 82
Bsp1286I GDGCHC 2 cut(s) 139, 220
Bsp19I CCATGG 1 cut(s) 83
BspANI GGCC 1 cut(s) 82
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 136
BspMAI CTGCAG 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 338
BssECI CCNNGG 1 cut(s) 83
BssT1I CCWWGG 1 cut(s) 83
Bst6I CTCTTC 2 cut(s) 165, 373
BstAPI GCANNNNNTGC 1 cut(s) 253
BstBAI YACGTR 1 cut(s) 242
BstDSI CCRYGG 1 cut(s) 83
BstF5I GGATG 2 cut(s) 305, 314
BstFNI CGCG 1 cut(s) 111
BstMAI GTCTC 1 cut(s) 3
BstMWI GCNNNNNNNGC 4 cut(s) 173, 182, 253, 275
BstSCI CCNGG 1 cut(s) 137
BstSFI CTRYAG 1 cut(s) 165
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 5 cut(s) 36, 110, 163, 184, 256
BstV2I GAAGAC 1 cut(s) 379
BsuRI GGCC 1 cut(s) 82
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 2 cut(s) 305, 314
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 162
Cfr13I GGNCC 1 cut(s) 424
Csp6I GTAC 1 cut(s) 61
CspCI CAANNNNNGTGG 2 cut(s) 21, 56
CviAII CATG 3 cut(s) 84, 125, 248
CviJI RGCY 6 cut(s) 82, 137, 148, 176, 185, 450
CviKI_1 RGCY 6 cut(s) 82, 137, 148, 176, 185, 450
CviQI GTAC 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 165, 373
EarI CTCTTC 2 cut(s) 165, 373
Eco130I CCWWGG 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 424
Eco72I CACGTG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 83
FaeI CATG 3 cut(s) 87, 128, 251
FaiI YATR 4 cut(s) 85, 126, 189, 249
FaqI GGGAC 2 cut(s) 57, 166
FatI CATG 3 cut(s) 83, 124, 247
Fnu4HI GCNGC 5 cut(s) 25, 99, 177, 198, 270
FokI GGATG 2 cut(s) 292, 301
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 5 cut(s) 25, 99, 177, 198, 270
FspBI CTAG 1 cut(s) 363
GluI GCNGC 5 cut(s) 25, 99, 177, 198, 270
GsaI CCCAGC 2 cut(s) 152, 314
HaeIII GGCC 1 cut(s) 82
HapII CCGG 1 cut(s) 139
Hin1II CATG 3 cut(s) 87, 128, 251
HinfI GANTC 2 cut(s) 11, 113
HpaII CCGG 1 cut(s) 139
Hpy188III TCNNGA 2 cut(s) 391, 431
HpyAV CCTTC 4 cut(s) 72, 125, 347, 437
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 4 cut(s) 167, 179, 224, 343
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 182, 253, 275
HpySE526I ACGT 1 cut(s) 241
Hsp92II CATG 3 cut(s) 87, 128, 251
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 6 cut(s) 134, 152, 270, 324, 388, 427
Lsp1109I GCAGC 5 cut(s) 36, 110, 163, 184, 256
LweI GCATC 2 cut(s) 323, 335
MaeI CTAG 1 cut(s) 363
MaeII ACGT 1 cut(s) 241
MboII GAAGA 6 cut(s) 182, 285, 291, 324, 379, 390
MhlI GDGCHC 2 cut(s) 139, 220
MluCI AATT 1 cut(s) 225
MlyI GAGTC 1 cut(s) 5
MnlI CCTC 8 cut(s) 14, 59, 66, 114, 166, 204, 295, 415
MseI TTAA 1 cut(s) 438
MspI CCGG 1 cut(s) 139
MspR9I CCNGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 4 cut(s) 173, 182, 253, 275
NciI CCSGG 1 cut(s) 139
NcoI CCATGG 1 cut(s) 83
NlaIII CATG 3 cut(s) 87, 128, 251
NlaIV GGNNCC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 5 cut(s) 26, 100, 178, 199, 271
PleI GAGTC 1 cut(s) 5
PmaCI CACGTG 1 cut(s) 242
PmlI CACGTG 1 cut(s) 242
PpsI GAGTC 1 cut(s) 5
Ppu21I YACGTR 1 cut(s) 242
PpuMI RGGWCCY 1 cut(s) 424
Psp5II RGGWCCY 1 cut(s) 424
PspCI CACGTG 1 cut(s) 242
PspFI CCCAGC 2 cut(s) 148, 310
PspN4I GGNNCC 1 cut(s) 136
PspPI GGNCC 1 cut(s) 424
PspPPI RGGWCCY 1 cut(s) 424
PstI CTGCAG 1 cut(s) 169
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 5 cut(s) 25, 99, 177, 198, 270
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 2 cut(s) 139, 220
SetI ASST 4 cut(s) 244, 417, 429, 452
SfaNI GCATC 2 cut(s) 323, 335
SfcI CTRYAG 1 cut(s) 165
SinI GGWCC 1 cut(s) 424
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
Sse9I AATT 1 cut(s) 225
SspMI CTAG 1 cut(s) 363
StyD4I CCNGG 1 cut(s) 137
StyI CCWWGG 1 cut(s) 83
TaiI ACGT 1 cut(s) 244
TaqI TCGA 2 cut(s) 206, 390
TasI AATT 1 cut(s) 225
TatI WGTACW 1 cut(s) 60
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TscAI CASTG 1 cut(s) 169
TseI GCWGC 5 cut(s) 24, 98, 176, 197, 269
TspDTI ATGAA 1 cut(s) 285
TspRI CASTG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 424
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.