Rh6BG312000

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005523
Physical Location & Seq
Reverse (-)
54159761 .. 54161203
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG312000.1

Sequence Viewer

Length: 441 bp
ATGAAAGAAGATCCCAGCAAAAGCAACAAGATAGAAGAGGAGGGACAGAAATATGTCAAGTACAGAGGAGTAAGAAGAAGGCCATGGGGAAAGTTTGCAGCAGAAATACGTGATTCGACAAGGCAAGGAGTTCGGTTGTGGCTTGGGACTTTTAATACTGCAAAAGAGGCTGCAAGGGCATATGACAAAGCCGCTTTCTCTATGAGGGGTCCTCTTGCAATTCTCAATTTCCCAAGTGAGCATGGTTTTGTATATGCTGATTCTGCTGCTACTTCCTCATCTTTCACAGCATCATCTTCAACACTGCATAACATGGAAGTGACAATGAGAACAGAACCCAGAAAAGAAGTTTTTGTGTTCGAATATCTAGATGACAAGGTTTTAGAGGAACTTCTTGATTTTGAAAACAATAGCAAGAGAGTGGAACTTGGTGTACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

146

Amino Acids

16.69

Weight (kDa)

7.93

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 20 - 70 1.9e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 192
AclWI GGATC 1 cut(s) 5
AfaI GTAC 2 cut(s) 62, 435
AgsI TTSAA 2 cut(s) 300, 404
AlwI GGATC 1 cut(s) 5
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 3 cut(s) 98, 170, 266
AspS9I GGNCC 1 cut(s) 209
AsuII TTCGAA 1 cut(s) 360
AvaII GGWCC 1 cut(s) 209
BbvI GCAGC 3 cut(s) 110, 157, 253
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BfaI CTAG 1 cut(s) 368
BisI GCNGC 4 cut(s) 99, 171, 192, 267
BlsI GCNGC 4 cut(s) 100, 172, 193, 268
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 210
BmsI GCATC 1 cut(s) 299
BplI GAGNNNNNCTC 2 cut(s) 196, 228
Bpu14I TTCGAA 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 83
BseRI GAGGAG 2 cut(s) 53, 81
BseXI GCAGC 3 cut(s) 110, 157, 253
BseYI CCCAGC 1 cut(s) 14
BshFI GGCC 1 cut(s) 82
BslFI GGGAC 2 cut(s) 57, 160
BsmFI GGGAC 2 cut(s) 57, 160
BsnI GGCC 1 cut(s) 82
Bsp119I TTCGAA 1 cut(s) 360
Bsp1407I TGTACA 1 cut(s) 433
Bsp143I GATC 1 cut(s) 10
Bsp19I CCATGG 1 cut(s) 83
BspACI CCGC 1 cut(s) 192
BspANI GGCC 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 210
BspPI GGATC 1 cut(s) 5
BspT104I TTCGAA 1 cut(s) 360
BsrGI TGTACA 1 cut(s) 433
BssECI CCNNGG 1 cut(s) 83
BssMI GATC 1 cut(s) 10
BssT1I CCWWGG 1 cut(s) 83
Bst6I CTCTTC 1 cut(s) 30
BstAUI TGTACA 1 cut(s) 433
BstBAI YACGTR 1 cut(s) 110
BstBI TTCGAA 1 cut(s) 360
BstDSI CCRYGG 1 cut(s) 83
BstKTI GATC 1 cut(s) 13
BstMBI GATC 1 cut(s) 10
BstMWI GCNNNNNNNGC 3 cut(s) 167, 176, 263
BstV1I GCAGC 3 cut(s) 110, 157, 253
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BsuRI GGCC 1 cut(s) 82
BtgI CCRYGG 1 cut(s) 83
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 61, 434
CviAII CATG 3 cut(s) 84, 242, 313
CviJI RGCY 4 cut(s) 82, 142, 170, 191
CviKI_1 RGCY 4 cut(s) 82, 142, 170, 191
CviQI GTAC 2 cut(s) 61, 434
DpnI GATC 1 cut(s) 12
DpnII GATC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 83
FaeI CATG 3 cut(s) 87, 245, 316
FaqI GGGAC 2 cut(s) 57, 160
FatI CATG 3 cut(s) 83, 241, 312
FauNDI CATATG 1 cut(s) 181
Fnu4HI GCNGC 4 cut(s) 99, 171, 192, 267
Fsp4HI GCNGC 4 cut(s) 99, 171, 192, 267
FspBI CTAG 1 cut(s) 368
GluI GCNGC 4 cut(s) 99, 171, 192, 267
GsaI CCCAGC 1 cut(s) 18
HaeIII GGCC 1 cut(s) 82
Hin1II CATG 3 cut(s) 87, 245, 316
HinfI GANTC 2 cut(s) 113, 260
Hpy166II GTNNAC 1 cut(s) 434
Hpy188III TCNNGA 2 cut(s) 368, 395
Hpy8I GTNNAC 1 cut(s) 434
HpyAV CCTTC 1 cut(s) 72
HpyCH4IV ACGT 1 cut(s) 109
HpyCH4V TGCA 5 cut(s) 98, 161, 173, 218, 307
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 176, 263
HpySE526I ACGT 1 cut(s) 109
Hsp92II CATG 3 cut(s) 87, 245, 316
Kzo9I GATC 1 cut(s) 10
LpnPI CCDG 2 cut(s) 28, 352
Lsp1109I GCAGC 3 cut(s) 110, 157, 253
LweI GCATC 1 cut(s) 299
MaeI CTAG 1 cut(s) 368
MaeII ACGT 1 cut(s) 109
MaeIII GTNAC 1 cut(s) 319
MalI GATC 1 cut(s) 12
MboI GATC 1 cut(s) 10
MboII GAAGA 4 cut(s) 20, 47, 87, 288
MflI RGATCY 1 cut(s) 10
MluCI AATT 2 cut(s) 219, 226
MnlI CCTC 8 cut(s) 31, 34, 59, 160, 198, 222, 286, 379
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 317
MwoI GCNNNNNNNGC 3 cut(s) 167, 176, 263
NcoI CCATGG 1 cut(s) 83
NdeI CATATG 1 cut(s) 181
NdeII GATC 1 cut(s) 10
NlaIII CATG 3 cut(s) 87, 245, 316
NlaIV GGNNCC 1 cut(s) 210
NmuCI GTSAC 1 cut(s) 319
NspV TTCGAA 1 cut(s) 360
PfeI GAWTC 2 cut(s) 113, 260
PkrI GCNGC 4 cut(s) 100, 172, 193, 268
Ppu21I YACGTR 1 cut(s) 110
PpuMI RGGWCCY 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 209
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 1 cut(s) 209
PspPPI RGGWCCY 1 cut(s) 209
PsrI GAACNNNNNNTAC 1 cut(s) 417
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 2 cut(s) 62, 435
RsaNI GTAC 2 cut(s) 61, 434
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 4 cut(s) 99, 171, 192, 267
Sau3AI GATC 1 cut(s) 10
Sau96I GGNCC 1 cut(s) 209
SetI ASST 2 cut(s) 112, 381
SfaNI GCATC 1 cut(s) 299
SfuI TTCGAA 1 cut(s) 360
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 317
Sse9I AATT 2 cut(s) 219, 226
SsiI CCGC 1 cut(s) 192
SspMI CTAG 1 cut(s) 368
StyI CCWWGG 1 cut(s) 83
TaiI ACGT 1 cut(s) 112
TaqI TCGA 2 cut(s) 116, 360
TasI AATT 2 cut(s) 219, 226
TatI WGTACW 2 cut(s) 60, 433
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 2 cut(s) 113, 260
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 3 cut(s) 98, 170, 266
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 309
VpaK11BI GGWCC 1 cut(s) 209
XbaI TCTAGA 1 cut(s) 367
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.