MD05G1198900.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005523
Physical Location & Seq
Reverse (-)
32673890 .. 32674324
435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1198900.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGAAAGAGGCGGCAAAGATAAAGAAGAGGGACAGAAAGATGTGAAGTATAGAGGTGTGAGAACAAGGCCGTGGGGGAAATTCGCAGCCGAAATACGCGATTCGACGAGGCAGGGAGCTCGGTTGTGGCTTGGCACCTTCAACACTGCTGAAGAAGCTGCAAGGGCTTATGATAGAGCTGCTTTTTCCATGAGGGGTCCTCTTGCAATCCTCAATTTTCCAATTGAATATGATTTGAAAGATGCTGATCAGTCTGCTGCTGCTGTTTCTTCGTCTTCATCTTCTTTGTCGTCGTCTTCGAGACCCCATAATGTGACCAGGACGGAATCCGGAAGAGAAATCTTCGAGTTTGAGTGTTTAGATGATAGTGTTTTAGAGGACCTTCTTGATTTTGACAATCATACCAAAACTAACGAAGAAAAACAAATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

145

Amino Acids

16.21

Weight (kDa)

5.3

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 16 - 66 5.9e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 99
AccIII TCCGGA 1 cut(s) 329
AciI CCGC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 171
AgsI TTSAA 3 cut(s) 142, 227, 238
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 3 cut(s) 119, 158, 179
AluI AGCT 3 cut(s) 119, 158, 179
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 5 cut(s) 86, 158, 179, 257, 260
ApoI RAATTY 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 197, 379
AvaII GGWCC 2 cut(s) 197, 379
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 267, 288
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 5 cut(s) 98, 145, 166, 244, 247
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 1 cut(s) 319
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 295
BisI GCNGC 6 cut(s) 13, 87, 159, 180, 258, 261
BlsI GCNGC 6 cut(s) 14, 88, 160, 181, 259, 262
Bme1390I CCNGG 1 cut(s) 319
Bme18I GGWCC 2 cut(s) 197, 379
BmgT120I GGNCC 2 cut(s) 197, 379
BmiI GGNNCC 2 cut(s) 136, 198
BmrFI CCNGG 1 cut(s) 319
BmsI GCATC 1 cut(s) 232
BpiI GAAGAC 2 cut(s) 267, 288
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BsaBI GATNNNNATC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 71
BsaWI WCCGGW 1 cut(s) 329
Bse8I GATNNNNATC 1 cut(s) 246
BseAI TCCGGA 1 cut(s) 329
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 71
BseJI GATNNNNATC 1 cut(s) 246
BseXI GCAGC 5 cut(s) 98, 145, 166, 244, 247
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 70
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 121
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 1 cut(s) 45
BsmAI GTCTC 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 1 cut(s) 70
Bso31I GGTCTC 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 121
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 1 cut(s) 247
BspACI CCGC 1 cut(s) 12
BspANI GGCC 1 cut(s) 70
BspEI TCCGGA 1 cut(s) 329
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 2 cut(s) 136, 198
BspT107I GGYRCC 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 319
Bst6I CTCTTC 2 cut(s) 21, 328
BstDSI CCRYGG 1 cut(s) 71
BstFNI CGCG 1 cut(s) 99
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 1 cut(s) 295
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 2 cut(s) 155, 164
BstNI CCWGG 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 317
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 5 cut(s) 98, 145, 166, 244, 247
BstV2I GAAGAC 2 cut(s) 267, 288
BsuRI GGCC 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 71
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 197, 379
CviAII CATG 1 cut(s) 190
CviJI RGCY 7 cut(s) 70, 89, 119, 130, 158, 167, 179
CviKI_1 RGCY 7 cut(s) 70, 89, 119, 130, 158, 167, 179
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 21, 328
EarI CTCTTC 2 cut(s) 21, 328
Ecl136II GAGCTC 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 121
Eco31I GGTCTC 1 cut(s) 295
Eco47I GGWCC 2 cut(s) 197, 379
Eco53kI GAGCTC 1 cut(s) 119
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 119
EcoO109I RGGNCCY 2 cut(s) 197, 379
EcoRII CCWGG 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 121
FaeI CATG 1 cut(s) 193
FaiI YATR 6 cut(s) 51, 171, 191, 231, 309, 402
FaqI GGGAC 1 cut(s) 45
FatI CATG 1 cut(s) 189
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 6 cut(s) 13, 87, 159, 180, 258, 261
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 6 cut(s) 13, 87, 159, 180, 258, 261
GluI GCNGC 6 cut(s) 13, 87, 159, 180, 258, 261
HaeIII GGCC 1 cut(s) 70
HapII CCGG 1 cut(s) 330
Hin1II CATG 1 cut(s) 193
HinfI GANTC 2 cut(s) 101, 326
HpaII CCGG 1 cut(s) 330
Hpy188III TCNNGA 3 cut(s) 300, 330, 386
Hpy99I CGWCG 2 cut(s) 109, 295
HpyAV CCTTC 2 cut(s) 148, 392
HpyCH4V TGCA 2 cut(s) 161, 206
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 164
Hsp92II CATG 1 cut(s) 193
Kpn2I TCCGGA 1 cut(s) 329
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 1 cut(s) 247
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 4 cut(s) 98, 304, 331, 343
Lsp1109I GCAGC 5 cut(s) 98, 145, 166, 244, 247
LweI GCATC 1 cut(s) 232
MaeIII GTNAC 1 cut(s) 313
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 9 cut(s) 38, 164, 261, 267, 273, 288, 334, 345, 428
MfeI CAATTG 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 121
MluCI AATT 4 cut(s) 80, 214, 222, 426
MnlI CCTC 7 cut(s) 22, 47, 102, 186, 210, 221, 370
MroI TCCGGA 1 cut(s) 329
MseI TTAA 1 cut(s) 429
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 319
MunI CAATTG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 2 cut(s) 155, 164
NdeII GATC 1 cut(s) 247
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 2 cut(s) 136, 198
NmuCI GTSAC 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PfeI GAWTC 2 cut(s) 101, 326
PkrI GCNGC 6 cut(s) 14, 88, 160, 181, 259, 262
PpuMI RGGWCCY 2 cut(s) 197, 379
Psp124BI GAGCTC 1 cut(s) 121
Psp5II RGGWCCY 2 cut(s) 197, 379
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 136, 198
PspPI GGNCC 2 cut(s) 197, 379
PspPPI RGGWCCY 2 cut(s) 197, 379
SacI GAGCTC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 6 cut(s) 13, 87, 159, 180, 258, 261
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 2 cut(s) 197, 379
ScrFI CCNGG 1 cut(s) 319
SduI GDGCHC 1 cut(s) 121
SetI ASST 6 cut(s) 58, 121, 140, 160, 181, 384
SfaNI GCATC 1 cut(s) 232
SinI GGWCC 2 cut(s) 197, 379
Sse9I AATT 4 cut(s) 80, 214, 222, 426
SsiI CCGC 1 cut(s) 12
SstI GAGCTC 1 cut(s) 121
StyD4I CCNGG 1 cut(s) 317
TaqI TCGA 3 cut(s) 104, 299, 345
TasI AATT 4 cut(s) 80, 214, 222, 426
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 2 cut(s) 101, 326
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 5 cut(s) 86, 158, 179, 257, 260
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 1 cut(s) 267
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 197, 379
XapI RAATTY 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.