pycom10g16020

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005523
Physical Location & Seq
Reverse (-)
19216967 .. 19217404
438 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g16020.1

Sequence Viewer

Length: 438 bp
ATGGAAAGAGGCCAAAGCAAAGGCAAAGCTAAAGAAGAGGGACAGAGAGATGTGAGGTATAGAGGTGTGAGAACAAGGCCGTGGGGGAAATTCGCTGCCGAAACACGCGATTCGACAAGGAAGGGAGGTCGGTTGTGGCTTGGCACCTTCAACACTGCTGAAGAAGCTGCAAGGGCTTATGATAGAGCTGCTTTTTCCATGAGGGGTGCTCTTGCAATTCTCAATTTTCCAAGTGAATATGGTTTGAAAGATGCAGGTCACTCTGCTGCTACTACTTCCTCATCATCTTCTTCGTCTTCATCTTCTTTTCCATCATCTTCGAGAATCCATAATGCGAGTAGGACCGAATTCGGAAGAGAAATCTTCGAGTTTGAGTGTTTAGATGATAGTGTTTTAGAGGACCTTCTTGATTTTGAGAATAATAGCAAAAGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

146

Amino Acids

16.06

Weight (kDa)

8.79

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 19 - 69 2.4e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 143
AccII CGCG 1 cut(s) 108
AcsI RAATTY 2 cut(s) 89, 347
AcuI CTGAAG 1 cut(s) 180
AgsI TTSAA 2 cut(s) 151, 247
AluBI AGCT 3 cut(s) 29, 167, 188
AluI AGCT 3 cut(s) 29, 167, 188
Alw21I GWGCWC 1 cut(s) 211
AoxI GGCC 2 cut(s) 10, 77
ApeKI GCWGC 4 cut(s) 95, 167, 188, 266
ApoI RAATTY 2 cut(s) 89, 347
AspS9I GGNCC 2 cut(s) 342, 400
AvaII GGWCC 2 cut(s) 342, 400
BanI GGYRCC 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 4 cut(s) 82, 154, 175, 253
BccI CCATC 1 cut(s) 319
BceAI ACGGC 1 cut(s) 64
BfuAI ACCTGC 1 cut(s) 245
BisI GCNGC 4 cut(s) 96, 168, 189, 267
BlsI GCNGC 4 cut(s) 97, 169, 190, 268
Bme18I GGWCC 2 cut(s) 342, 400
BmgT120I GGNCC 2 cut(s) 342, 400
BmiI GGNNCC 1 cut(s) 145
BmsI GCATC 1 cut(s) 241
BpiI GAAGAC 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 193, 225
BsaJI CCNNGG 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 80
BseXI GCAGC 4 cut(s) 82, 154, 175, 253
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 12, 79
BshNI GGYRCC 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 211
BslFI GGGAC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 2 cut(s) 12, 79
Bsp1286I GDGCHC 1 cut(s) 211
BspANI GGCC 2 cut(s) 12, 79
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 80
Bst6I CTCTTC 2 cut(s) 30, 349
BstDSI CCRYGG 1 cut(s) 80
BstFNI CGCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 2 cut(s) 164, 173
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 4 cut(s) 82, 154, 175, 253
BstV2I GAAGAC 1 cut(s) 288
BsuRI GGCC 2 cut(s) 12, 79
BtgI CCRYGG 1 cut(s) 80
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 1 cut(s) 153
BveI ACCTGC 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 342, 400
CviAII CATG 1 cut(s) 199
CviJI RGCY 7 cut(s) 12, 29, 79, 139, 167, 176, 188
CviKI_1 RGCY 7 cut(s) 12, 29, 79, 139, 167, 176, 188
Eam1104I CTCTTC 2 cut(s) 30, 349
EarI CTCTTC 2 cut(s) 30, 349
Eco47I GGWCC 2 cut(s) 342, 400
Eco57I CTGAAG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 400
EcoRI GAATTC 1 cut(s) 347
FaeI CATG 1 cut(s) 202
FaiI YATR 5 cut(s) 60, 180, 200, 240, 330
FaqI GGGAC 1 cut(s) 54
FatI CATG 1 cut(s) 198
Fnu4HI GCNGC 4 cut(s) 96, 168, 189, 267
Fsp4HI GCNGC 4 cut(s) 96, 168, 189, 267
GluI GCNGC 4 cut(s) 96, 168, 189, 267
HaeIII GGCC 2 cut(s) 12, 79
Hin1II CATG 1 cut(s) 202
HinfI GANTC 2 cut(s) 110, 324
Hpy188I TCNGA 1 cut(s) 353
Hpy188III TCNNGA 2 cut(s) 321, 407
HpyAV CCTTC 3 cut(s) 115, 157, 413
HpyCH4V TGCA 3 cut(s) 170, 215, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 173
Hsp92II CATG 1 cut(s) 202
LpnPI CCDG 1 cut(s) 240
Lsp1109I GCAGC 4 cut(s) 82, 154, 175, 253
LweI GCATC 1 cut(s) 241
MaeIII GTNAC 1 cut(s) 257
MboII GAAGA 9 cut(s) 47, 173, 279, 282, 288, 294, 309, 355, 366
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 4 cut(s) 89, 216, 223, 347
MnlI CCTC 7 cut(s) 31, 48, 56, 119, 195, 289, 391
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 164, 173
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 110, 324
PkrI GCNGC 4 cut(s) 97, 169, 190, 268
PpuMI RGGWCCY 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 145
PspPI GGNCC 2 cut(s) 342, 400
PspPPI RGGWCCY 1 cut(s) 400
SatI GCNGC 4 cut(s) 96, 168, 189, 267
Sau96I GGNCC 2 cut(s) 342, 400
SduI GDGCHC 1 cut(s) 211
SetI ASST 9 cut(s) 31, 59, 67, 130, 149, 169, 190, 259, 405
SfaNI GCATC 1 cut(s) 241
SinI GGWCC 2 cut(s) 342, 400
Sse9I AATT 4 cut(s) 89, 216, 223, 347
TaqI TCGA 3 cut(s) 113, 320, 366
TaqII GACCGA 1 cut(s) 359
TasI AATT 4 cut(s) 89, 216, 223, 347
TfiI GAWTC 2 cut(s) 110, 324
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 1 cut(s) 257
TseI GCWGC 4 cut(s) 95, 167, 188, 266
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 1 cut(s) 288
TspRI CASTG 1 cut(s) 160
VpaK11BI GGWCC 2 cut(s) 342, 400
XapI RAATTY 2 cut(s) 89, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.