RchiOBHm_Chr6g0288281

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005523
Physical Location & Seq
Reverse (-)
51549217 .. 51549696
480 bp
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UTR
Exon/CDS
Intron
PRQ25866

Sequence Viewer

Length: 480 bp
ATGGATTCACTGATTCACATTCAGTTAGTGCTATCCGATATGAAAGAAGATCCCAGCAAAAGCAAGAAGATAGAAGATGAGGGACAGAAATATGTCAAGTACAGAGGAGTAAGAAGAAGGCCATGGGGAAAGTTTGCAGCAGAAATACGCGATTCGACAAGGCAAGGAGTTCGGTTGTGGCTTGGGACTTTTAATACTGCAAAAGAGGCTGCAAGGGCATATGACAAAGCCGCTTTCTCTATGAGGGGTCCTCTTGCAATTCTCAATTTCCCAAGTGAGCATGGTTTTGTATATGTTGATTCTGCTCCTACTTCCTCATCTTTCACAGCATCATCTTCAACACTGCATAACATGGAAGTGACAATGAGAACAGAACCCAGAAAAGAAGTTTTTGTGTTCGAGTATCTAGATGACAAGGTTTTAGAGGAACTTCTTGATTTTGAAAACAATAGCAAGAGAGTGGAACTTGGTGTACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

159

Amino Acids

18.21

Weight (kDa)

6.85

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 33 - 83 2.2e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 150
AciI CCGC 1 cut(s) 231
AclWI GGATC 1 cut(s) 44
AfaI GTAC 2 cut(s) 101, 474
AgsI TTSAA 2 cut(s) 339, 443
AlwI GGATC 1 cut(s) 44
AoxI GGCC 1 cut(s) 119
ApeKI GCWGC 2 cut(s) 137, 209
AspS9I GGNCC 1 cut(s) 248
AvaII GGWCC 1 cut(s) 248
BbvI GCAGC 2 cut(s) 149, 196
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BfaI CTAG 1 cut(s) 407
BisI GCNGC 3 cut(s) 138, 210, 231
BlsI GCNGC 3 cut(s) 139, 211, 232
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 249
BmsI GCATC 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 235, 267
BsaJI CCNNGG 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 120
BseXI GCAGC 2 cut(s) 149, 196
BseYI CCCAGC 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 1 cut(s) 121
BslFI GGGAC 2 cut(s) 96, 199
BsmFI GGGAC 2 cut(s) 96, 199
BsnI GGCC 1 cut(s) 121
Bsp1407I TGTACA 1 cut(s) 472
Bsp143I GATC 1 cut(s) 49
Bsp19I CCATGG 1 cut(s) 122
BspACI CCGC 1 cut(s) 231
BspANI GGCC 1 cut(s) 121
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 1 cut(s) 44
BsrGI TGTACA 1 cut(s) 472
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 122
BstAUI TGTACA 1 cut(s) 472
BstDSI CCRYGG 1 cut(s) 122
BstFNI CGCG 1 cut(s) 150
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 2 cut(s) 206, 215
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 149, 196
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 121
BtgI CCRYGG 1 cut(s) 122
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 2 cut(s) 8, 341
Cfr13I GGNCC 1 cut(s) 248
Csp6I GTAC 2 cut(s) 100, 473
CviAII CATG 3 cut(s) 123, 281, 352
CviJI RGCY 4 cut(s) 121, 181, 209, 230
CviKI_1 RGCY 4 cut(s) 121, 181, 209, 230
CviQI GTAC 2 cut(s) 100, 473
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 248
EcoO109I RGGNCCY 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 3 cut(s) 126, 284, 355
FaqI GGGAC 2 cut(s) 96, 199
FatI CATG 3 cut(s) 122, 280, 351
FauNDI CATATG 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 138, 210, 231
Fsp4HI GCNGC 3 cut(s) 138, 210, 231
FspBI CTAG 1 cut(s) 407
GluI GCNGC 3 cut(s) 138, 210, 231
GsaI CCCAGC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 121
Hin1II CATG 3 cut(s) 126, 284, 355
HinfI GANTC 4 cut(s) 5, 13, 152, 299
Hpy166II GTNNAC 1 cut(s) 473
Hpy188I TCNGA 1 cut(s) 37
Hpy188III TCNNGA 2 cut(s) 407, 434
Hpy8I GTNNAC 1 cut(s) 473
HpyAV CCTTC 1 cut(s) 111
HpyCH4V TGCA 5 cut(s) 137, 200, 212, 257, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 215
Hsp92II CATG 3 cut(s) 126, 284, 355
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 2 cut(s) 67, 391
Lsp1109I GCAGC 2 cut(s) 149, 196
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 5 cut(s) 59, 79, 86, 126, 327
MflI RGATCY 1 cut(s) 49
MluCI AATT 2 cut(s) 258, 265
MnlI CCTC 7 cut(s) 73, 98, 199, 237, 261, 325, 418
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 356
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 206, 215
NcoI CCATGG 1 cut(s) 122
NdeI CATATG 1 cut(s) 220
NdeII GATC 1 cut(s) 49
NlaIII CATG 3 cut(s) 126, 284, 355
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 358
PfeI GAWTC 4 cut(s) 5, 13, 152, 299
PkrI GCNGC 3 cut(s) 139, 211, 232
PpuMI RGGWCCY 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 248
PspPPI RGGWCCY 1 cut(s) 248
PsrI GAACNNNNNNTAC 1 cut(s) 456
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 2 cut(s) 101, 474
RsaNI GTAC 2 cut(s) 100, 473
RseI CAYNNNNRTG 1 cut(s) 356
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 3 cut(s) 138, 210, 231
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 248
SetI ASST 1 cut(s) 420
SfaNI GCATC 1 cut(s) 338
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 356
Sse9I AATT 2 cut(s) 258, 265
SsiI CCGC 1 cut(s) 231
SspMI CTAG 1 cut(s) 407
StyI CCWWGG 1 cut(s) 122
TaqI TCGA 2 cut(s) 155, 399
TasI AATT 2 cut(s) 258, 265
TatI WGTACW 2 cut(s) 99, 472
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 4 cut(s) 5, 13, 152, 299
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 2 cut(s) 15, 348
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 137, 209
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 1 cut(s) 56
TspRI CASTG 2 cut(s) 15, 348
VpaK11BI GGWCC 1 cut(s) 248
XbaI TCTAGA 1 cut(s) 406
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.